Search Results (3,616)

Shell-like housing structures for motors and compressors can be found in everyday products. Consumers significantly evaluate acoustic emissions during the first usage of products. Unpleasant sounds may raise concerns and cause complaints to be issued. A prevention strategy is a holistic acoustic design, which includes predicting the emitted sound power as part of end-of-line testing. The hybrid experimental-simulative sound power prediction based on laser scanning vibrometry (LSV) is ideal in acoustically harsh production environments. However, conducting vibroacoustic testing with laser scanning vibrometry is time-consuming, making it difficult to fit into the production cycle time. This contribution discusses how the time-consuming sampling process can be accelerated to estimate the radiated sound power, utilizing adaptive sampling. The goal is to predict the acoustic signature and its uncertainty from surface velocity data in seconds. Fulfilling this goal will enable integration into a product assembly unit and final acoustic quality control without the need for an acoustic chamber. The Gaussian process regression based on PyTorch 2.6.0 performed 60 times faster than the preliminary reference implementation, resulting in a regression estimation time of approximately one second for each frequency bin. In combination with the Equivalent Radiated Power prediction of the sound power, a statistical measure is available, indicating how the uncertainty of a limited number of surface velocity measurement points leads to predictions of the uncertainty inside the acoustical signal. An adaptive sampling algorithm reduces the prediction uncertainty in real-time during measurement. The method enables on-the-fly error analysis in production, assessing the risk of violating agreed-upon acoustic sound power thresholds, and thus provides valuable feedback to the product design units. Full article

With the continuous improvement of the prefabricated modular technology system, the prefabricated subway station structures are widely used in underground engineering projects. However, prefabricated subway stations in soft soil can suffer significant adverse effects under seismic action. In order to study the seismic performance of a prefabricated subway station, this work is based on an actual project of a subway station in soft soil. And the nonlinear static and dynamic coupling two-dimensional finite element models of cast-in-place structures (CIPs), assembly splicing structures (ASSs), and assembly monolithic structures (AMSs) are established, respectively. The soil-structure interaction is considered, and different peak ground accelerations (PGA) are selected for incremental dynamic analysis. The displacement response, internal force characteristics, and structural damage distribution for three structural forms are compared. The research results show that the inter-story displacement of the AMS is slightly greater than that of the CIP, while the inter-story displacement of the ASS is the largest. The CIP has the highest internal force in the middle column, the ASS has the lowest internal force in the middle column, and the AMS is between the two. The damage to the CIP is concentrated at the bottom of the middle column and sidewall. The AMS compression damage moves upward, but the tensile damage mode is similar to the CIP. The ASS can effectively reduce damage to the middle column and achieve redistribution of internal force. Further analysis shows that the joint splicing interface between cast-in-place and prefabricated components is the key to controlling the overall deformation and seismic performance of the structure. The research results can provide a theoretical basis for the seismic design optimization of subway stations in earthquake-prone areas. Full article

Elucidating amyloid formation inside biomolecular condensates requires models that resolve (i) local, chemistry specific contacts controlling β registry and (ii) mesoscale phase behavior and cluster coalescence on microsecond timescales—capabilities beyond single resolution models. We present a hybrid united atom/coarse grained (UA–CG) force field coupling a PACE UA peptide model with the MARTINI CG framework. Cross resolution nonbonded parameters are first optimized against all atom side chain potentials of mean force to balance the relative strength between different types of interactions and then refined through universal parameter scaling by matching radius of gyration distributions for specific systems using. We applied this approach to simulate a recently reported model system comprising the LVFFAR₉ peptide that can co-assemble into amyloid fibrils via liquid–liquid phase separation. Our ten-microsecond simulations reveal rapid droplet formation populated by micelle like nanostructures with its inner core composed of LVFF clusters. The nanostructures can further fuse but the fusion is reaction-limited due to an electrostatic coalescence barrier. β structures emerge once clusters exceed ~10 peptides, and the LVFFAR₉ fraction modulates amyloid polymorphism, reversing parallel versus antiparallel registry at lower LVFFAR₉. These detailed insights generated from long simulations highlight the promise of our hybrid UA–CG strategy in investigating the molecular mechanism of condensate aging. Full article

The microhylid frog Microhyla fissipes is a protected terrestrial wildlife species in China, recognized for its ecological, economic, and scientific value. However, its mitochondrial genome remains poorly characterized. To address this gap, we sequenced and annotated the complete mitogenome of M. fissipes to elucidate its structural organization and phylogenetic placement within Microhylidae. The assembled mitogenome is 16,723 bp in length and contains 37 genes, including 13 protein-coding genes, 2 rRNAs, and 22 tRNAs, along with one control region and the origin of heavy-strand replication. We also identified eight overlapping regions and eleven intergenic spacers. The overall base composition showed an A + T bias (59.91%) with negative AT-skew (−0.04) and GC-skew (−0.27). All tRNAs displayed typical cloverleaf secondary structures, except for trnS1, which lacked the D-arm. Phylogenetic reconstruction using both maximum likelihood and Bayesian inference strongly supported the monophyly of Microhylidae and revealed a sister-group relationship between Microhyla and Kaloula. Within Microhyla, M. fissipes was most closely related to M. heymonsi, with which it formed a well-supported clade that also included Microhyla okinavensis, Microhyla mixtura, and Microhyla beilunensis. Selection pressure analysis on protein-coding genes indicated widespread purifying selection (Ka/Ks < 1) across most genes, except for ATP8, COX2, and COX3, which may be under relaxed selective constraints. These findings offer valuable genomic resources for the conservation of M. fissipes and provide new insights into the phylogeny and evolution of microhylid frogs. Full article

This paper presents a novel mechanically beam-steered antenna system for 10 GHz applications, enabled by multi-material 3D-printing technology. The proposed design eliminates the need for complex electronic circuitry by integrating a mechanically rotatable, 3D-printed hemispherical lens with a conventional rectangular patch antenna. The system comprises three main components: a 10-GHz patch antenna, a precision-fabricated hemispherical dielectric lens produced via stereolithography (SLA), and a structurally robust rotation assembly fabricated using fused deposition modeling (FDM). The mechanical rotation of the lens enables discrete beam-steering from −45° to +45° in 5° steps. Experimental results demonstrate a gain improvement from 6.21 dBi (standalone patch) to 10.47 dBi with the integrated lens, with minimal degradation across steering angles (down to 9.59 dBi). Simulations and measurements show strong agreement, with the complete system achieving 94% accuracy in beam direction. This work confirms the feasibility of integrating additive manufacturing with passive beam-steering structures to deliver a low-cost, scalable, and high-performance alternative to electronically scanned arrays. Moreover, the design is readily adaptable for motorized actuation and closed-loop control via embedded systems, enabling future development of real-time, programmable beam-steering platforms. Full article

The global transition to a circular bioeconomy is accelerating the demand for sustainable, high-performance materials. Filamentous fungi represent a promising solution, as they function as living foundries that transform low-value biomass into advanced, self-assembling materials. While mycelium-based composites have proven potential, progress has been predominantly driven by empirical screening of fungal species and substrates. To unlock their full potential, a paradigm shift from empirical screening to rational design is required. This review introduces a conceptual framework centered on the biochemical programming of the fungal cell wall. Viewed through a materials science lens, the cell wall is a dynamic, hierarchical nanocomposite whose properties can be deliberately tuned. We analyze the contributions of its principal components—the chitin–glucan structural scaffold, the glycoprotein functional matrix, and surface-active hydrophobins—to the bulk characteristics of mycelium-derived materials. We then identify biochemical levers for controlling these properties. External factors such as substrate composition and environmental cues (e.g., pH) modulate cell wall architecture through conserved signaling pathways. Complementing these, an internal synthetic biology toolkit enables direct genetic and chemical intervention. Strategies include targeted engineering of biosynthetic and regulatory genes (e.g., CHS, AGS, GCN5), chemical genetics to dynamically adjust synthesis during growth, and modification of surface chemistry for specialized applications like tissue engineering. By integrating fungal cell wall biochemistry, materials science, and synthetic biology, this framework moves the field from incidental discovery toward the intentional creation of smart, functional, and sustainable mycelium-based materials—aligning material innovation with the imperatives of the circular bioeconomy. Full article

Polymeric vesicles, characterized by enhanced colloidal stability, excellent mechanical properties, controllable surface functionality, and adjustable membrane thickness, are extremely useful in nano- and bio-technology for potential applications as nanosized carriers for drugs and enzymes. However, a few preparative steps are necessary to achieve a unilamellar vesicle with a narrow size distribution. Herein, we report the spontaneous formation of unilamellar polymeric vesicles with nanometer sizes (<50 nm), fabricated by simply mixing diblock copolymers (P(EO-AGE)(2K-2K) and P(EO-AGE)(0.75K-2K)) with differing hydrophilic mass fractions in aqueous solutions. Depending on the mixing ratio of block copolymers and the temperature, the block copolymer mixtures self-assemble into various nanostructures, such as spherical and cylindrical micelles, or vesicles. The self-assembled structures of the block copolymer mixtures were characterized by small-angle neutron scattering, resulting in a phase diagram drawn as a function of temperature and the mixing condition. Notably, the critical temperature for the micelle-to-vesicle phase transition can be easily controlled by altering the mixing conditions; it decreases with an increase in the concentration of one of the block copolymers. Full article

Protein hydrolysates have potential as sustainable functional feed ingredients or additives for the aquaculture industry. This study examined the growth-promoting effects of duck-blood protein hydrolysate (DBPH, <10 kDa) on the flowerhorn cichlid (Amphilophus citrinellus × Cichlasoma trimaculatum). Fish with an average weight of 3.24 ± 0.22 g were randomly assigned to four dietary treatments: a negative control (basal diet) and basal diets supplemented with 0.5%, 1%, and 2% DBPH. After 90 days of the feeding trial, growth parameters indicated that supplementation with 1% and 2% DBPH enhanced growth. However, the muscle composition and skin coloration did not differ significantly. Transcriptome sequencing of the liver tissue yielded 39.83 GB of high-quality clean data. De novo transcriptome assembly identified 32,824 unigenes, of which 21,385 were successfully annotated based on public databases. Differential expression analysis identified 269 upregulated and 232 downregulated genes. To clarify the growth-promoting effects of DBPH, five glycolysis/gluconeogenesis-related genes (tpi, gapdh, pck1, ldh, and adh) were validated by liver qRT-PCR, and the results were consistent with those of the transcriptomic analysis. These findings provide new insights into the mechanisms by which DBPH supplementation could enhance growth, as evidenced by alterations in glycolysis and gluconeogenesis pathways, indicating potential as a novel feed additive in aquaculture. Full article

The optimization of trajectories for multiple robotic arms in a shared workspace is critical for industrial automation but presents significant challenges, including data sharing, communication overhead, and adaptability in dynamic environments. Traditional centralized control methods require sharing raw sensor data, raising concerns and creating computational bottlenecks. This paper proposes a novel Federated Learning (FL) framework for distributed multi-robotic arm trajectory optimization. Our method enables collaborative learning where robots train a shared model locally and only exchange gradient updates, preserving data privacy. The framework integrates an adaptive Rapidly exploring Random Tree (RRT) algorithm enhanced with a dynamic pruning strategy to reduce computational overhead and ensure collision-free paths. Real-time synchronization is achieved via EtherCAT, ensuring precise coordination. Experimental results demonstrate that our approach achieves a 17% reduction in average path length, a 22% decrease in collision rate, and a 31% improvement in planning speed compared to a centralized RRT baseline, while reducing inter-robot communication overhead by 45%. This work provides a scalable and efficient solution for collaborative manipulation in applications ranging from assembly lines to warehouse automation. Full article

Background: The quest for well-defined immunoadjuvants remains one of the highest priorities for the successful development of effective vaccines. Combination adjuvants, which are designed to integrate both the ability to activate a variety of immune mechanisms and synergistically improve the delivery of vaccine components, are well-positioned to address the unmet needs. The development of a preventive vaccine against hepatitis C virus (HCV)—a major public health concern—is a particular instance in which the choice of the immunoadjuvant is of utmost importance. Methods: We assembled a lipid A Toll-like receptor 4 (TLR4) agonist BECC438 and TLR7/8 agonist resiquimod (R848) on a polyphosphazene macromolecule (PCPP) to create a nanoscale immunoadjuvant-vaccine delivery system: PCPP-R+BECC438. This aqueous-based system was formulated with the HCV sE2 antigen, and the resulting vaccine candidate was evaluated in vivo for the ability to induce immune responses. Results: Co-assembly of adjuvants resulted in a visually clear aqueous system of nanoscale dimensions, monomodal size distribution, and entropy-driven interactions between components. Intramuscular immunization of mice with HCV sE2 antigen formulated in a polyphosphazene-based nano-system induced ten-fold higher IgG and IgG2a titers than the antigen adjuvanted with BECC438 alone. PCPP-R+BECC438 formulated HCV sE2 also produced statistically significant improvements in IgG2a/IgG1 ratio and more robust HCVpp neutralization ID₅₀ titers than control formulations. Conclusions: Polyphosphazene-assembled adjuvant nano-system promotes in vivo immune responses of enhanced quantity and quality of antibodies with increased potency of HCV neutralization. Full article

Sphenophorus levis, commonly known as the sugarcane weevil, is one of the most important pests affecting Brazilian sugarcane crops. It has spread to all sugarcane-producing regions of Brazil, mainly through contaminated stalks. Effective control of this pest is difficult due to the protection conferred by the host plant during the larval stage. As a result, despite current control measures, S. levis populations continue to grow, and reports of new infestations remain frequent. Biotechnological control measures, such as the use of viruses, stands as a promising tool for pest control in agriculture. The aim of this study was to explore the RNA virome associated with S. levis using a viral metagenomic approach. Through the Read Annotation Tool (RAT) pipeline, we characterized, for the first time, the gut-associated viral community in adult weevils, identifying several novel viral genomes. Sphenophorus levis-associated virus (SLAV) had 12,414 nucleotides (nt); Sphenophorus levis tombus-like virus (SLTV) had 4085 nt; and the four genomic segments of Sphenophorus levis reo-like virus (SLRV) ranged from 2021 to 4386 nt. These genomes were assembled from 65,759 reads (SLAV), 114,441 reads (SLTV), and 270,384 reads (SLRV). Among the detected viral families, Partitiviridae was the most abundant. The identification of possible viral pathogens lays the foundation for future research into their potential use as biological control agents against S. levis. Full article

Podocytopathy, characterized by proteinuria, contributes significantly to kidney diseases, with hypertension playing a key role in damaging podocytes and the glomerular filtration barrier (GFB). The lack of functional in vitro models, however, impedes research and treatment development for hypertensive podocytopathy. We established a novel constant pressure-driven podocyte-on-chip model, utilizing our previously developed dynamic staining self-assembly cell array chip (SACA chip) and 3D printing. This platform features a differentiated podocyte monolayer under controlled hydrostatic pressures, mimicking the epithelial side of the GFB. Using this platform, we investigated mechanical force-dependent permeability to three sizes of fluorescent dextran under varying hydrostatic pressures, comparing the results with a puromycin aminonucleoside (PAN)-induced injury model. We observed that external pressures induced size-dependent permeability changes and altered cell morphology. Higher pressures led to greater macromolecule infiltration, especially for larger dextran (70 kDa, 500 kDa). Mature podocytes exhibited immediate, pressure-dependent cytoskeleton rearrangements, with better recovery at lower pressures (20 mmHg) but irreversible injury at higher pressures (40, 60 mmHg). These morphological changes were also corroborated by dynamic mRNA expression of cytoskeleton-associated proteins, Synaptopodin and ACTN4. This platform offers a promising in vitro tool for investigating the pathomechanisms of hypertension-induced podocytopathy, performing on-chip studies of the GFB, and conducting potential drug screening. Full article

This study reports the synthesis and characterization of well-defined ionic graft conjugates acting as drug delivery systems, based on monomeric ionic units derived from choline methacrylate (TMAMA) biofunctionalized with the anions of ampicillin (AMP) or cloxacillin (CLX). Using the “grafting from” technique with multifunctional macroinitiators, the density of side chains was precisely defined, and the length of side chains was well-controlled during polymerization. The resulting ionic conjugates featured the regulated content of ionic fractions with drug anions reaching up to 55% and drug content up to 48–70% for AMP, 27–65% for CLX, and 47–79% for (CLX + AMP). The drug release behavior was evaluated under physiological conditions using a dialysis method. The ionic conjugates demonstrated release efficiencies of 70–93% for CLX (5–16 µg/mL), 69–98% for AMP (12–13 µg/mL) in single systems, and 61–73% for CLX + AMP (10–15 µg/mL) in dual systems. Additionally, polymer surface properties were evaluated via water contact angle measurements (WCA = 30–54°). In an aqueous solution, the polymer self-assemblies appeared to be nanosized particles (90–360 nm). The results demonstrate that the synthesized TMAMA-based graft copolymers act as effective ionic conjugates and dual drug systems, offering a promising platform for controlled and multi-drug delivery applications. Full article

Aphids remain exceptional models for symbiosis research due to their unique experimental advantages that extend beyond documenting symbiont-mediated phenotypes. Nine commonly occurring facultative bacterial symbionts provide well-characterized benefits, including defense against parasitoids, pathogens, and thermal stress. Yet the system’s greatest value lies in enabling diverse research applications across biological disciplines through experimental tractability combined with ecological realism. Researchers can create controlled experimental lines through symbiont manipulation, maintain clonal host populations indefinitely, and cultivate symbionts independently. This experimental power is complemented by extensive knowledge of symbiont dynamics in natural populations, including temporal and geographic distribution patterns—features generally unavailable in other insect-microbe systems. These advantages facilitate investigation of key processes in symbiosis, including transmission dynamics, mechanisms, strain-level functional diversity, multi-partner infections, and transitions from facultative to co-obligate relationships. Integration across biological scales—from genomics to field ecology—enables research on symbiont community assembly, ecological networks, coevolutionary arms races, and agricultural applications. This combination of experimental flexibility, comprehensive natural history knowledge, and applied relevance positions aphids as invaluable for advancing symbiosis theory while addressing practical challenges in agriculture and invasion biology. Full article

This research focuses on the synthesis, characterization, and photocatalytic performance of Cu-based polyoxometalate (Cu-POM) as an effective catalyst for the degradation of organic dyes, specifically Congo Red (CR) and Phenol Red (PR). The main goals are to synthesize Cu-POM using a controlled self-assembly technique, characterize its optical and structural characteristics using FTIR, XRD, SEM, TGA, and UV-Vis spectroscopy, and estimate its photocatalytic activity when exposed to UV light. The outcomes confirm the successful formation of Cu-POM with well-defined nanostructures and a crystalline polyoxometalate framework. The determined optical bandgap of 3.65 eV indicates its strong UV-light responsiveness. The photocatalytic degradation experiments demonstrated high removal efficiencies of 58.1% for CR and 64.6% for PR under UV irradiation, corresponding kinetic rate constants of 0.00484 min⁻¹ and 0.00579 min⁻¹, respectively. The superior photocatalytic activity is attributed to the efficient charge carrier separation and high surface area of Cu-POM. These findings highlight the potential of Cu-POM as a promising heterogeneous photocatalyst for sustainable wastewater treatment and environmental remediation. Full article