Search Results (68)

The aim of this study was to elucidate the reproductive strategy of Camellia luteoflora, an endangered evergreen endemic to karst ecosystems. We observed and recorded its flowering phenology and flower-visiting insects, observed pollen morphology, determined pollen viability, and assessed stigma receptivity. The results showed that the flowering period of C. luteoflora started from early September to late December, with the average flowering period of individual flowers being 10–12 days. The pollen morphology of C. luteoflora was subprolate and prolate, with three germinal apertures and the fossulate exine ornamentation. Pollen viability was the highest at the initial opening stage (80.30%). In the process of pollen in vitro, the order of influence on the germination rate and pollen tube length was temperature > sucrose > calcium chloride (CaCl₂) > boric acid (H₃BO₃). The best combination for the germination rate was 24 °C, 75 g/L sucrose, 0.2 g/L CaCl₂, 0.15 g/L H₃BO₃, while that for the pollen tube length was 24 °C, 100 g/L sucrose, 0.2 g/L CaCl₂, 0.25 g/L H₃BO₃. Stigma receptivity was the strongest at the full blooming stage. The pollen/ovule ratio (P/O) was 2240, suggesting a facultative outcrossing breeding system. The outcrossing index (OCI) was 4, suggesting that the exogamous breeding system is the cross-pollination type, partially self-compatible and insect pollinator-dependent. The flower-visiting insects included bees, weevils, and ants. In summary, C. luteoflora exhibits an extended flowering period, with a prolonged overlap of stable pollen viability and stigma receptivity, suggesting a potential strategy to cope with pollination uncertainty. However, field observations recorded only a few species of potential pollinators, while the occurrence frequency of non-pollinating insects was relatively high. It is thus hypothesized that this apparent lack of effective pollinators may act as a potential barrier to successful fertilization and natural regeneration, which might also be one of the factors contributing to its endangered status. Future studies, particularly pollinator exclusion and hand-pollination experiments, are critically needed to verify whether pollinator limitation is indeed a key factor. Full article

Consistency indices quantify the degree of transitivity and proportionality violations in a pairwise comparison matrix (PCM), forming a cornerstone of the Analytic Hierarchy Process (AHP) and Analytic Network Process (ANP). Several methods have been proposed to compute consistency, including those based on the maximum eigenvalue, dot product, Jaccard index, and the Bose index. However, these methods often overlook two critical aspects: (i) vector projection or directional alignment, and (ii) the weight or importance of individual elements within a pointwise evaluative structure. The first limitation is particularly impactful. Adjustments made during the consistency improvement process affect the final priority vector disproportionately when heavily weighted elements are involved. Although consistency may improve numerically through such adjustments, the resulting priority vector can deviate significantly, especially when the true vector is known. This indicates that approaches neglecting projection and weighting considerations may yield internally consistent yet externally incompatible vectors, thereby compromising the validity of the analysis. This study builds on the idea that consistency and compatibility are intrinsically related; they are two sides of the same coin and should be considered complementary. To address these limitations, it introduces a novel metric, the Consistency Index G (CI-G) based on the compatibility index G. This measure evaluates how well the columns of a PCM align with its principal eigenvector, using CI-G as a diagnostic component. The proposed approach not only refines consistency measurement but also enhances the accuracy and reliability of derived priorities. Full article

A new conformally invariant gravitational generalization of the Born–Infeld (BI) model is proposed and analyzed from the point of view of symmetries. Taking a geometric identity involving the determinant functions det$f\left(B_{\mu \nu },F_{\mu \nu }\right)$ with the Bach $B_{\mu \nu }$ and the electromagnetic field $F_{\mu \nu }$ tensors (with the 4-dimensional Greek letter indexes), two characteristic geometrical Lagrangian densities (Lagrangians) are derived: the first Lagrangian being the square root of the determinant function $\sqrt{\left|det\left(B_{\mu \nu }+F_{\mu \nu }\right)\right|}$ (reminiscent of the standard BI model) and the second Lagrangian being the fourth root $\sqrt[4]{gdet\left(B_{\alpha \gamma }{B}_{\beta }^{\gamma }+F_{\alpha \gamma }{F}_{\beta }^{\gamma }\right)}$. It is shown, after explicit computation of the gravitational equations, that the square-root model is incompatible with the inclusion of the electromagnetic tensor, consequently forcing the nullity of $F_{\mu \nu }$. In sharp contrast, the traceless fourth-root model is fully compatible and a natural ansatz of the type $B_{\mu \rho }{B}_{\nu }^{\rho }\propto \mathrm{\Omega }\left(x\right)g_{\mu \nu }$ (conformal-Killing), with $\mathrm{\Omega }$ the conformal factor and x the 4-coordinate, can be considered. Among other essential properties, the geometrical conformal Lagrangian of the fourth-root type is self-similar with respect to the determinant g of the metric tensor $g_{\mu \nu }$ and can be extended to non-Abelian fields in a way similar to the model developed by the author earlier. This self-similarity is related to the conformal properties of the model, such as the Bach currents or flows presumably of a topological origin. Possible applications and comparisons with other models are briefly discussed. Full article

Bacillus velezensis is an effective biocontrol bacterium, with its microbial pesticides showing promise in biological control. In this study, we optimized the medium and conditions for fermenting strain F0b, developed a wettable powder formulation, and assessed its efficacy against Botrytis cinerea. We screened carriers, wetting agents, dispersants, and UV protectants compatible with F0b, determining the optimal ratio and dosage. The best medium for F0b fermentation included rice flour (3.472%), ammonium chloride (4.898%), and disodium phosphate (1.871%). The ideal fermentation conditions were a 20% inoculum volume, 40 °C temperature, 80% sterile water, and a 72 h fermentation time, yielding a viable count of 1.33 × 10¹⁰ CFU/mL. The final formulation contained 54.7% Bacillus velezensis dried powder, 27.3% kaolinite carrier, 16% wetting agent (3:7 ratio of sodium dodecyl sulfate to sodium lignin sulfonate), and 2% ascorbic acid as a UV protectant. All quality indicators met national standards, with a viable bacteria concentration of 7 billion CFU/g. Field trials showed that the F0b wettable powder effectively controlled Botrytis cinerea, with a disease index significantly lower than the control group. Control efficacy ranged from 50.58% to 73.14% over 7 to 14 days, demonstrating the commercial potential of this formulation. Full article

Bioremediation using microorganisms offers a sustainable approach to addressing hydrocarbon contamination. This study explores biosurfactant production by Yarrowia lipolytica IMUFRJ 50682 during crude oil and asphaltene-free fraction biodegradation in corn steep liquor (CSL)-based media. By evaluating CSL concentrations (5–30 g/L) and combinations with glucose, molasses, and crude oil, this study demonstrates that CSL is an effective nutrient source for supporting microbial growth and biosurfactant production. The highest emulsification index (EI = 73.3%) was achieved with 20 g/L of CSL after 48 h, while media containing mixed carbon sources (glucose and crude oil) enhanced metabolic efficiency, yielding a maximum cell growth of 8 g/L after 150 h. Despite inhibiting cell growth, the asphaltene-free fraction promoted biosurfactant activity, with the EI reaching 35.8% after 264 h. The results emphasize the importance of pH control, with the optimal emulsification being observed at pH ~6. This work highlights the potential of CSL as a cost-effective and sustainable additive, advancing applications in bioremediation and biosurfactant production and contributing to the development of environmentally compatible hydrocarbon degradation strategies. Full article

Polypropylene (PP) has a wide range of applications in daily life but it is highly flammable. Intumescent flame retardants (IFRs) are used to improve the flame-retardant performance of polypropylene. However, the poor compatibility between IFRs and PP poses significant challenges. In this study, an IFR was reacted with γ-aminopropyl triethoxysilane (KH550) to introduce necessary reactive sites on the surface of the IFR. Subsequently, maleic anhydride-grafted SBS (SBS-g-MAH) was reacted with KH550 to further coat the IFR, resulting in a modified IFR named MA-IFR. The effects of MA-IFR on the flame retardancy, mechanical properties, and water resistance of PP composites were systematically investigated. The limiting oxygen index of the PP/MA-IFR composite reached up to 39.7%, with the vertical burning test (UL-94) achieving a V-0 rating. Moreover, compared to the control PP, the peak heat release rate and peak smoke release rate were reduced by 85.0% and 82.5%, respectively. In addition, the mechanical properties of the PP composites were significantly improved, with tensile strength and impact strength increasing by 29% and 18%, respectively, compared to those of the PP/IFR composite. Notably, the PP/MA-IFR composite maintained excellent flame retardancy, even after being immersed in water at 70 °C for 168 h. These results demonstrate that MA-IFR offers a promising solution for producing flame-retardant and water-resistant PP composites. Full article

In the work presented here, we explore the upcycling of polyethylene terephthalate (PET) that was derived from water bottles. The material was granulated and extruded into a filament compatible with fused filament fabrication (FFF) additive manufacturing platforms. Three iterations of PET combined with a thermoplastic elastomer, styrene ethylene butylene styrene with a maleic anhydride graft (SEBS-g-MA), were made with 5, 10, and 20% by mass elastomer content. The elastomer and specific mass percentages were chosen based on prior successes involving acrylonitrile butadiene styrene (ABS), in which the maleic anhydride graft enabled compatibility between different materials. The rheological properties of PET and the PET/SEBS blends were characterized by the melt flow index and dynamic mechanical analysis. The addition of SEBS-g-MA did not have a significant impact on mechanical properties, as determined by tensile and impact testing, where all test specimens were manufactured by FFF. Delamination of the tensile specimens convoluted the ability to discern differences in the mechanical properties, particularly % elongation. Annealing of the specimens enabled the observation of the effect of elastomer content on the mechanical properties, particularly in the case of impact testing, where the impact strength increased with the increase in SEBS content. However, annealing led to shrinkage of the specimens, detracting from the realized benefits of the thermal process. Scanning electron microscopy of spent tensile specimens revealed that, in the non-annealed condition, SEBS formed nodules that would detach from the PET matrix during the tensile test, indicating that a robust bond was not present. The addition of SEBS-g-MA did allow for shape memory property characterization, where deformation of tensile specimens occurred at room temperature. Specimens from the 20% by mass elastomer content sample group exhibited a shape fixation ratio on the order of 99% and a shape recovery ratio on the order of 80%. This work demonstrates a potential waste reduction strategy to tackle the problem of polymer waste by upcycling discarded plastic into a feedstock material for additive manufacturing with shape memory properties. Full article

Aim: To investigate the anti-inflammatory, antioxidant, and diabetic wound healing properties of the novel topical formulation [Ferulic acid-loaded nanoemulgel (DLMGO-G)]. Methods: Ferulic acid nanoemulsion developed with lemongrass oil is investigated in diabetic wound healing. Further nanoemulsion is incorporated into 1% carbopol^® 934 to obtain the DLMGO-G. Nanoemulsion was characterized for particle size, and polydispersity index (PDI) was obtained by Malvern Zetasizer (Zetasizer Nano ZS, Malvern, AL, USA), and morphology by TEM (JEM 1400, JOEL, Akishima, Japan). Furthermore, in vitro cell line and in vivo studies were carried out. Results: The developed nanoemulsion showed a globule size of 28.04 ± 0.23 nm and PDI of 0.07 ± 0.01. The morphology of nanoformulations by TEM confirmed the spherical and uniform nature. Further, the nanoformulation in in vitro cell line experiments revealed that the IC50 value was increased by 1.52 times compared to the drug solution. The treatment groups have shown that fibroblast morphologies were spindle-shaped, suggesting that nanoformulation was compatible with the cells and developed normally on nanoformulation. It also reduced ROS with improved internalization more than the control group. The in vitro wound healing model also revealed that nanoformulation had better wound healing activity. In the in vivo diabetic wound studies on male SD rats, the levels of inflammatory markers such as TNF-α, IL-6, IL-22, and IL-1β declined significantly when treated with DLMGO-G. IL-10 levels significantly increased compared to the diseased group, and MMP-9 levels were remarkably decreased compared to the diseased group. Furthermore, histopathological studies showed the regeneration and granulation of tissues. Conclusions: Thus, these findings indicate that FA-loaded nanoemulgel greatly accelerates the healing of wounds in diabetic rats. Full article

Radio Determination Satellite Service (RDSS) is a characteristic service of BeiDou, which can provide users with short message communication services. Since the working frequency of an RDSS system is close to that of a 5G system, the RDSS system is very susceptible to interference from 5G out-of-band radiation. This paper analyzes the compatibility of 5G interference with an RDSS system from the perspective of the signal and the system. Firstly, the compatibility assessment is carried out from the perspective of the signal, the impact of interference on the capture and tracking performance of BeiDou is illustrated, and the safe coexistence distance of the two systems from the perspective of capture probability is obtained from the perspective of the signal. Subsequently, based on the link budget criterion, the interference of 5G base stations and 5G terminals to RDSS receivers under different frequency isolation and the required distance isolation for safe coexistence are analyzed from the system perspective. Finally, from the perspective of civil aviation safety, the aggregate interference is used as an evaluation index to evaluate the interference suffered by the aircraft during takeoff and landing and to obtain the interference suffered by the ground-based 5G base station during the takeoff and landing of the aircraft on different routes and in different 5G propagation environments. The simulation results show that when the airplane is closer to the ground, the ground 5G base stations will cause harmful interference to the RDSS receiver. In this study, the real flight data are combined with the simulation model to obtain the exact influence range of 5G interference on the RDSS system under different viewpoints. Full article

Exploring the interface of environmental sustainability and civil infrastructure development, this study introduces waste butter (WB), a byproduct of animal fat processing, as a novel bio-modifier in asphalt production. This approach not only recycles animal waste but also charts a course for sustainable infrastructural development, contributing to a reduced environmental impact and promoting circular economy practices. The experiments incorporated varying WB concentrations (e.g., 3%, 6%, and 9% by weight of binder) into standard AP-5 asphalt, employing advanced analytical tools for comprehensive characterization. These included thin-layer chromatography–flame ionization detection (TLC-FID), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and Differential Scanning Calorimetry (DSC). The critical properties of the asphalt blends, such as penetration, softening point, viscosity, ductility, rutting factor (Dynamic Shear Rheometer), and thermal susceptibility (Penetration Index, Penetration–Viscosity Number), were assessed. FT-IR analysis indicated negligible chemical alteration with WB addition, suggesting predominantly physical interactions. TLC-FID showed a decrease in aromatic and asphaltene components but an increase in resin content, highlighting the influence of WB’s fatty acids on the asphalt’s chemical balance. The colloidal instability index (I_C) confirmed enhanced stability due to WB’s high resin concentration. Meanwhile, SEM analysis revealed microstructural improvements with WB, enhancing binder compatibility. TGA demonstrated that even a minimal 3 wt. % WB addition significantly improved thermal stability, while the DSC results pointed to improved low-temperature performance, reducing brittleness in cold conditions. Rheologically, WB incorporation resulted in increased penetration and ductility, balanced by decreased viscosity and softening point, thereby demonstrating its multi-faceted utility. Thermal susceptibility tests emphasized WB’s effectiveness in cold environments, with further evaluation needed at higher temperatures. The DSR findings necessitate careful WB calibration to meet Superpave rutting standards. In conclusion, this research positions waste butter as a superior, environmentally aligned bio-additive for asphalt blends, contributing significantly to eco-friendly civil engineering practices by repurposing animal-derived waste. Full article

Background/Objectives: Erosive hand osteoarthritis (EHOA) is an aggressive form of hand osteoarthritis (OA) and a severely disabling condition. Patients affected by OA frequently lament symptoms suggestive of neuropathic pain (NP). The aim of our study was to ascertain the presence and severity of NP in patients with EHOA and correlate its presence with EHOA clinical characteristics. Methods: In this retrospective study, we included all consecutive EHOA patients with NP symptoms who underwent upper limb electroneurography (ENoG) and nerve ultrasound. The presence of NP was screened using the ID pain neuropathic pain-screening questionnaire (ID-Pain). In addition, the following NP questionnaires were also used: Douleur Neuropathique en 4 Questions (DN4), PainDETECT, and Neuropathic Pain Symptom Inventory (NPSI). Moreover, patients completed the Australian/Canadian Osteoarthritis Hand Index (AUSCAN) and Dreiser’s algofunctional finger index questionnaires assessing EHOA disease activity. The following clinical and laboratory data were collected: age, sex, BMI, disease duration, intensity of pain (VAS 0–10), painful and swollen joints, and inflammatory indices, as well as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Results: Of the 34 patients studied, 24 (70.6%) presented NP to the ID-Pain questionnaire. According to DN4, 14 (41.2%) patients had NP, while using the PainDETECT questionnaire, 67.6% had NP. Patients with NP were statistically younger and had a higher VAS pain score compared to subjects without NP. The ENoG and median nerve ultrasound were normal in 81% of patients, while four patients had carpal tunnel syndrome. The ID-Pain questionnaire correlated with the number of painful joints (r = 0.48, p = 0.03) and with the AUSCAN questionnaire (r = 0.37, p = 0.05). The DN4 questionnaire correlated with PainDETECT (r = 0.58, p < 0.01). The PainDETECT questionnaire correlated with VAS pain (r = 0.49, p = 0.02), the DN4 questionnaire (r = 0.58, p < 0.01), and AUSCAN (r = 0.51, p = 0.02). The NPSI questionnaire correlated negatively with BMI (r = −0.53, p = 0.01) and positively with the PainDETECT questionnaire (r = 0.49, p = 0.02). Conclusions: Our study revealed that 32% to 70% of EHOA patients exhibited symptoms consistent with NP, with observed variability depending on the questionnaire utilized. Despite patients frequently exhibiting symptoms compatible with NP, only 19% of patients presented alterations on ENoG and ultrasound examinations confirming CTS. This suggests a probable nociplastic component for pain in patients with EHOA, which warrants tailored treatment. In the present study, NP correlated with clinical and functional indices of EHOA. Full article

Graphitic carbon nitride (g-C₃N₄) is an intriguing nanomaterial that exhibits photoconductive fluorescence properties under UV–visible light. Dopamine (DA) coating of g-C₃N₄ prepared from melamine was accomplished via self-polymerization of DA as polydopamine (PDA). The g-C₃N₄ was coated with PDA 1, 3, and 5 times repeatedly as (PDA@g-C₃N₄) in tris buffer at pH 8.5. As the number of PDA coatings was increased on g-C₃N₄, the peak intensity at 1512 cm⁻¹ for N–H bending increased. In addition, the increased weight loss values of PDA@g-C₃N₄ structures at 600 °C from TGA thermograms confirmed that the coating was accomplished. The band gap of g-C₃N₄, 2.72 eV, was reduced to 0.87 eV after five coatings with PDA. A pristine g-C₃N₄ was found to have an isoelectric point (IEP) of 4.0, whereas the isoelectric points of 1PDA@g-C₃N₄ and 3PDA@g-C₃N₄ are close to each other at 3.94 and 3.91, respectively. On the other hand, the IEP of 5PDA@g-C₃N₄ was determined at pH 5.75 assuming complete coating with g-C₃N₄. The biocompatibility of g-C₃N₄ and PDA@g-C₃N₄ against L929 fibroblast cell lines revealed that all PDA@g-C₃N₄ coatings were found to be biocompatible up to a 1000 mg/mL concentration, establishing that PDA coatings did not adversely affect the biocompatibility of the composite materials. In addition, PDA@g-C₃N₄ was screened for antioxidant potential via total phenol content (TPC) and total flavonoid content assays and it was found that PDA@g-C₃N₄ has recognizable TPC values and increased linearly with an increased number of PDA coatings. Furthermore, blood compatibility of pristine g-C₃N₄ is enhanced considerably upon PDA coating, affirmed by hemolysis and the blood clotting index%. Additionally, α-glucosidase inhibitory properties of PDA@g-C₃N₄ structures revealed that 67.6 + 9.8% of this enzyme was evenly inhibited by 3PDA@g-C₃N₄ structure. Full article

A high-sensitivity silicon microring (Si MRR) optical biosensor for detecting the nucleocapsid protein of SARS-CoV-2 is proposed and demonstrated. In the proposed biosensor, the surface of a Si MRR waveguide is modified with antibodies, and the target protein is detected by measuring a resonant wavelength shift of the MRR caused by the selective adsorption of the protein to the surface of the waveguide. A Si MRR is fabricated on a silicon-on-insulator substrate using a CMOS-compatible fabrication process. The quality factor of the MRR is approximately 20,000. The resonant wavelength shift of the MRR and the detection limit for the environmental refractive index change are evaluated to be 89 nm/refractive index unit (RIU) and 10⁻⁴ RIU, respectively. The sensing characteristics are examined using a polydimethylsiloxane flow channel after the surface of the Si MRR waveguide is modified with the IgG antibodies through the Si-tagged protein. First, the selective detection of the protein by the MRR sensor is experimentally demonstrated by the detection of bovine serum albumin and human serum albumin. Next, various concentrations of nucleocapsid protein solutions are measured by the MRR, in which the waveguide surface is modified with the IgG antibodies through the Si-tagged protein. Although the experimental results are very preliminary, they show that the proposed sensor has a potential nucleocapsid sensitivity in the order of 10 pg/mL, which is comparable to the sensitivity of current antigen tests. The detection time is less than 10 min, which is much shorter than those of other antigen tests. Full article

The recognition of pollen and pistil in the self-incompatibility process is generally determined by the interaction between the pollen S gene and pistil S gene located at the S locus. However, the regulatory mechanism of self-incompatibility in goji remains unknown. In this study, we used the self-compatible strain ‘13–19’ and self-incompatible strain ‘xin9’ from Ningxia as parents to create an F1 hybrid population. Reciprocal cross-pollination was performed within the same plant to evaluate the self-compatibility of the parents and F1 progeny. The parents and progeny were subjected to whole-genome resequencing, and mixed pools of DNA were constructed using 30 self-compatible and 30 self-incompatible individuals. Association analysis using the SNP-index method and Euclidean distance was employed to identify the key candidate region of the S locus. The candidate region was further annotated using the Swiss-Prot database to identify genes within the region. Additionally, transcriptome sequencing data from different organs/tissues, as well as from pistils of self-compatible and self-incompatible strains at control (0 h), short (0.5 h), medium (8 h), and long (48 h) time points after self-pollination and cross-pollination, were analyzed to assess differential gene expression and screen for self-compatibility-related loci. Specific primers were designed for PCR amplification to determine the S-RNase genotypes of the extreme parents. The results revealed that the S locus in goji is located within a 32.2 Mb region on chromosome 2 that contains a total of 108 annotated genes. Differential expression analysis showed that ten genes, including Lba02g01064, were specifically expressed in stamens, with four of them annotated as F-box genes, potentially serving as determinants of self-compatibility in stamens. Lba02g01102 was exclusively expressed in pistils and annotated as an S-RNase gene, likely involved in self-compatibility. The expression of Lba02g01102 in pistils decreased after self-pollination and cross-pollination. Six candidate genes exhibited significant changes after self-pollination and cross-pollination. Both parents and progeny carried two S-RNase alleles, and the S-RNase genotypes showed a significant correlation with self-compatibility, with the self-compatible progeny containing the S8-RNase allele. The identification of the S locus in goji provides molecular markers for future marker-assisted breeding and offers genetic resources for studying the mechanism of self-incompatibility in goji, thus contributing to the improvement of goji varieties. Full article

Polypropylene (PP) is one of the most extensively used commodity plastics. In terms of eco-friendliness, it is worth considering preparing high-lignin-filled PP. This study explores the incorporation of high lignin content, derived from acetic acid lignin (AAL) and Kraft lignin (KL), into PP through twin-screw extrusion and injection molding. The challenge lies in maintaining mechanical performance. A compatibilizer—specifically, maleic anhydride-grafted polypropylene (MAPP)—is employed to enhance lignin–PP compatibility by chemically bonding with lignin and physically associating with the PP phase. Results indicate that KL maintains better dispersity than AAL. Compatibilizers with a high maleic anhydride (MA) level (≥0.8 wt.%) and moderate melt flow index (MFI) in the range of 60–100 g 10 min⁻¹ prove favorable in constructing a reinforced PP/KL network. Optimizing with 40 wt.% lignin content and 10 parts per hundred (pph) of compatibilizer yields blends with mechanical performance comparable to neat PP, exhibiting a notable increase in modulus and heat deflection temperature (HDT). Furthermore, utilizing PP/lignin blends can lead to a 20% reduction in expenses and approximately 40% reduction in PP-induced greenhouse gas (GHG) emissions. This approach not only reduces PP costs but also adds value to lignin utilization in a sustainable and cost-effective manner. Full article