Search Results (72)

Forests are considered one of the most valuable natural areas in metropolitan region landscapes. Considering the sensitivity and ecosystem services provided by trees, the definition of urban forest ecosystems is nowadays based on a comprehensive understanding of the entire urban ecosystem. The effective capturing of particulate matter is one of the ecosystem services provided by urban forests. These ecosystems function as efficient biological filters. Plants accumulate pollutants passively via their leaves. Therefore, another ecosystem service provided by city forests could be the use of tree organs as bioindicators of pollution. This paper aims to estimate differences in trace metal pollution between the wooded urban areas of Vienna and Krakow using leaves of evergreen and deciduous trees as biomonitors. An additional objective of the research was to assess the ability of the applied tree species to act as biomonitors. Plant samples of five species—Norway spruce, Scots pine, European larch, common white birch, and common beech—were collected within both areas, in seven locations: four in the “Wienerwald” Vienna forest (Austria) and three in the “Las Wolski” forest in Krakow (Poland). Concentrations of Cr, Cu, Cd, Pb, and Zn in plant material were determined. Biomonitoring studies with deciduous and coniferous tree leaves showed statistically higher heavy metal contamination in the “Las Wolski” forest compared to the “Wienerwald” forest. Based on the conducted analyses and the literature study, it can be concluded that among the analyzed tree species, only two: European beech and common white birch can be considered potential indicators in environmental studies. These species appear to be suitable bioindicators, as both are widespread in urban woodlands of Central Europe and have shown the highest accumulation levels of trace metals. Full article

Secure autonomous secret key distillation (SKD) systems traditionally depend on external common randomness (CR) sources, which often suffer from instability and limited reliability over long-term operation. In this work, we propose a novel SKD architecture that synthesizes CR by combining a keystream of a shared-key keystream generator $KSG\left(K_G\right)$ with locally generated binary Bernoulli noise. This construction emulates the statistical properties of the classical Maurer satellite scenario while enabling deterministic control over key parameters such as bit error rate, entropy, and leakage rate (LR). We derive a closed-form lower bound on the equivocation of the shared-secret key $K_G$ from the viewpoint of an adversary with access to public reconciliation data. This allows us to define an admissible operational region in which the system guarantees long-term secrecy through periodic key refreshes, without relying on advantage distillation. We integrate the Winnow protocol as the information reconciliation mechanism, optimized for short block lengths ($N=8$), and analyze its performance in terms of efficiency, LR, and final key disagreement rate (KDR). The proposed system operates in two modes: ideal secrecy, achieving secret key rates up to 22% under stringent constraints (KDR < 10⁻⁵, LR < 10⁻¹⁰), and perfect secrecy mode, which approximately halves the key rate. Notably, these security guarantees are achieved autonomously, without reliance on advantage distillation or external CR sources. Theoretical findings are further supported by experimental verification demonstrating the practical viability of the proposed system under realistic conditions. This study introduces, for the first time, an autonomous CR-based SKD system with provable security performance independent of communication channels or external randomness, thus enhancing the practical viability of secure key distribution schemes. Full article

The co-disposal of municipal solid waste incineration fly ash (MSWI-FA) in cement kilns is an effective method for managing incineration by-products in China. However, the presence of heavy metals in MSWI-FA raises environmental concerns. This study analyzed the Cu, Zn, Cd, Pb, Cr, and Ni concentrations in MSWI-FA from 11 representative facilities across China and assessed the efficacy of a three-stage water washing process for Cl and heavy metal removal. The results revealed significant regional variations in heavy metal content that were strongly correlated with surface soil levels, with Zn, Pb, and Cu exhibiting the highest concentrations. Elemental correlations, such as Cu-Pb and Zn-Cd synergies and Cd-Ni antagonism, suggest common waste sources and temperature-dependent volatilization during incineration. The washing process (solid–liquid ratio = 1:10) achieved 97.1 ± 2.0% Cl removal, reducing residual Cl to 0.45 ± 0.32%, but demonstrated limited heavy metal elimination (10.28–19.38% efficiency), resulting in elevated concentrations (32.5–60.8% increase) due to 43.4 ± 9.2% mass loss. Notably, the washing effluents exceeded municipal wastewater discharge limits by up to 52-fold for Pb and 38-fold for Cd, underscoring the need for advanced effluent treatment. To mitigate environmental risks, the addition of washed MSWI-FA in cement kilns should be restricted to ≤0.5%, with Cd content prioritized in pre-disposal assessments. This study provides actionable insights for optimizing MSWI-FA co-processing while ensuring compliance with ecological safety standards. Full article

Objectives: Carbapenem-resistant Gram-negative bacteria (CR-GNB) infections in intensive care units (ICUs) are increasingly prevalent and associated with high mortality. This study aimed to investigate the distribution of isolated bacteria and determine the factors associated with mortality among ICU patients diagnosed with CR-GNB infections. Methods: This retrospective study included 95 patients admitted to the ICU between February 2022 and July 2024 who were diagnosed with CR-GNB infections via culture and initiated on treatment. Thirty-day mortality was defined as the clinical outcome, and patients were divided into two groups: survivors (Group 1, n = 42) and deceased (Group 2, n = 53). Demographic, clinical, laboratory, and microbiological data were analyzed. Results: Advanced age, the presence of malignancy, an elevated Charlson Comorbidity Index (CCI), lower platelet counts, and higher C-reactive protein (CRP) levels were significantly associated with mortality (p < 0.05). Trauma-related admissions were more common among survivors, while sepsis-related admissions predominated among non-survivors. No statistically significant associations were observed between antibiotic regimen type and mortality. Culture-based pathogen distribution revealed A. baumannii as the predominant organism in respiratory samples, while K. pneumoniae was more frequently isolated from bloodstream and urinary specimens. Conclusions: Mortality in ICU patients with CR-GNB infections is influenced by both baseline comorbidities and infection-related inflammatory markers. This study provides region-specific insights from a high-resistance ICU setting and may inform risk stratification, prognostication, and management strategies in critically ill patients with CR-GNB infections. Full article

Background: Lonchodidae is the largest family within the order Phasmatodea, and although many studies have been conducted on this family, the monophyly of the family has not been established. Methods: Eight mitogenomes from Lonchodidae, including the first complete mitogenomes of four genera, were sequenced and annotated to explore their features and phylogenetic relationships. Results: The total length ranged from 15,942–18,021 bp, and the mitogenome consisted of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a control region (CR). atp8 had the highest A + T content in Lonchodidae, except for Neohirasea stephanus and Asceles clavatus, in which the highest A + T contents were detected in nad6. The phylogenetic trees were reconstructed via Bayesian inference (BI) and maximum likelihood (ML) based on the PCG123 and PCG12 datasets. As the phylogenetic trees show, Necrosciinae is recognized as monophyletic, but the monophyly of Lonchodinae has not been supported. Gene deletion and rearrangement have occurred mainly in Lonchodidae and Aschiphasmatidae. The most common reason for gene rearrangements was tandem duplication random loss (TDRL), but trnI of Stheneboea repudiosa inverted into the CR. In addition, genes within the same family or genus share related sequences and conserved gene blocks. Conclusions: we expanded the mitochondrial genomic data for this family, thereby establishing a foundational dataset for future studies. Full article

The assessment of surface water quality of the Danube River and Black Sea was performed taking into account the amounts determined for heavy metals (As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, Zn), nutrients (compounds of N and P, chlorophyll a), emerging contaminants (CECs) (pharmaceutics and endocrine disruptors—19 quantified compounds, out of 30 targeted chemicals), heterotrophic bacteria and total coliforms, in thirty-two locations from the lower Danube sector (starting with km 375 up to the river mouths), the Danube Delta Biosphere Reserve (three Danube branches—Chilia, Sulina, and Sf. Gheorghe) and the Romanian coastal area of the Black Sea. The heavy metals levels were found in the following ranges: 3.0–6.5 μg/L As; 0.51–1.32 μg/L Cd; 21.6–61.2 μg/L Cr; 10.2–28.6 μg/L Cu; 196–351 μg/L Mn; 12.3–47.67 μg/L Ni; 5.2–15.5 μg/L Pb; 44–74 μg/L Zn; 0.01–0.08 μg/L Hg. The nutrient concentrations vary in the intervals: 0.04–0.45 mg/L N-NH₄; 0.01–0.06 mg/L N-NO₂; 0.07–1.9 mg/L N-NO₃; 1.0–3.2 mg/L N total; 0.01–0.05 mg/L P-PO₄; 0.02–0.27 mg/L P total, and 0.8–17.3 μg/L chlorophyll a. The concentrations of CECs from various classes (sulfamethoxazole, trimethoprim, ciprofloxacin, flumequine, amoxicillin, cefuroxime, dicloxacillin, carbamazepine, pravastatin, erythromycin, piroxicam, ketoprofen, diclofenac, naproxen, enilconazole (imazalil), clotrimazole, drospirenone, 17α-ethinylestradiol, and bisphenol A) were compared with values reported for European rivers and the Danube River water in various river sectors. The highest detection frequencies were registered for bisphenol A (100%), sulfamethoxazole (96%), carbamazepine and diclofenac (87%), trimethoprim (78%), pravastatin (46%), and imazalil (34%). Bisphenol A exhibited the largest concentrations (342 ng/L), followed by diclofenac (132 ng/L), carbamazepine (38 ng/L), and sulfamethoxazole (36 ng/L). For most of the contaminants, Black Sea coastal water showed lower concentrations than the Danube water and good ecological status for surface water. Correlations between CECs and total coliforms suggest insufficient treated wastewater effluents as a common contamination source and possible use of CECs as indirect fecal pollution indicator in aquatic systems. This is the first study carried out in the connected system Danube River–Danube Delta–Black Sea for a large palette of toxicants classes and microbial pollutants, which will serve as a baseline for future monitoring of water quality in the region. Full article

Nipah virus (NiV) is a severe zoonotic pathogen that substantially threatens public health. Pigs are the natural hosts of NiV and can potentially transmit this disease to humans. Establishing a rapid, sensitive, and accurate point-of-care detection method is critical in the timely identification of infected pig herds. In this study, we developed an NiV detection method based on reverse transcription–recombinase polymerase amplification (RT-RAA) and the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 13a (Cas13a) system for the precise detection of NiV. The highly conserved region of the NiV gene was selected as the detection target. We first designed eleven pairs of RT-RAA primers, and the optimal primer combination and reaction temperature were identified on the basis of RT-RAA efficiency. Additionally, the most efficient crRNA sequence was selected on the basis of the fluorescence signal intensity. The results revealed that the optimal reaction temperature for the developed method was 37 °C. The detection limit was as low as 1.565 copies/μL. Specificity testing revealed no cross-reactivity with nucleic acids from six common swine viruses, including Seneca virus A (SVA), foot-and-mouth disease virus (FMDV), classical swine fever virus (CSFV), porcine epidemic diarrhea virus (PEDV), African swine fever virus (ASFV), and pseudorabies virus (PRV). A validation test using simulated clinical samples revealed a 100% concordance rate. The detection results can be visualized via a fluorescence reader or lateral flow strips (LFSs). Compared with conventional detection methods, this RT-RAA-CRISPR/Cas13a-based method is rapid and simple and does not require scientific instruments. Moreover, the reagents can be freeze-dried for storage, eliminating the need for cold-chain transportation. This detection technology provides a convenient and efficient new tool for the point-of-care diagnosis of NiV and for preventing and controlling outbreaks. Full article

Walnut canker is a common disease in the Xinjiang Uygur autonomous region of China, which is caused by Cytospora chrysosperma. To date, there is no effective control measure for this disease. Infection with mycoviruses has been widely proven to reduce the virulence of plant pathogenic fungi, with some mycoviruses even serving as potential biological control agents for plant diseases. In this study, mycoviruses associated with 31 strains of C. chrysosperma from Xinjiang Uygur autonomous region were identified by metatranscriptomic sequencing. Seven new mycoviruses were identified by BLAST and RT-PCR analysis, which were Botrytis cinerea partitivirus 5 (BcPV5), Gammapartitivirus sp-XJ1 (GVsp-XJ1), Botoulivirus sp-XJ2 (BVsp-XJ2), Luoyang Fusar tick virus 2 (LfTV2), Leptosphaeria biglobosa narnavirus 17 (LbNV17), Sclerotinia sclerotiorum narnavirus 6 (SsNV6), and Cytospora ribis mitovirus (CrMV3). Among these, BcPV5, GVsp-XJ1, BVsp-XJ2, CrMV3, and LfTV2 were found to co-infect C. chrysosperma strain WS-11 and significantly reduce both the colony growth rate and virulence of the host. After co-culturing the virus-free WS-FV strain with WS-11, the colony growth rate and virulence of the derivative strain were also decreased. These results provide potential biocontrol resources for the control of walnut canker. Full article

The origin and bioavailability of nickel (Ni), chromium (Cr), and cobalt (Co) have been assessed in the Čemernica River basin among the following soil types: Leptosol on serpentinite and Fluvisol, Vertisol, and Leptosol on sandstone. Alongside the impact of serpentine rocks, part of the region also displays significant anthropogenic activity. A sequential analysis following the modified BCR procedure identified four fractions of heavy metals: F1—exchangeable and acid-soluble, F2—bound to Fe and Mn oxides, F3—bound to organic matter, and F4—residual. The overall content of Ni, Cr, and Co in the soil increases in the following order: Leptosol on sandstone, and Vertisol, Fluvisol, Leptosol on serpentinite. In most samples, the values exceed the maximum allowable concentrations in legal regulations, while in some samples, particularly in serpentine soils, they also exceed the remediation thresholds. The average contribution of nickel in the overall content decreases as follows: F4 > F2 > F3 > F1, chromium as F4 > F3 > F2 > F1, and cobalt in the most samples as F2 > F4 > F1 > F3. The percentage of Ni, Cr, and Co in the fractions of different soil types is similar to that in serpentine soils, though the absolute contents vary significantly, indicating a common origin, which is mostly geochemical. According to the Risk Assessment Code, most samples exhibit a low bioavailability risk. Full article

Linden (Tilia) is one of the most frequently utilized plants for the preparation of infusions because of its salutary effects, including the reduction in inflammatory processes and pain, alleviation of stress, and lowering of blood pressure. As Linden is a common species in Bulgarian cities, it is frequently used for homemade infusions. The regular consumption of these tea beverages may contribute to the attainment of the recommended daily allowances of certain minerals, but it may also result in the accumulation of toxic elements within the human body. The present study compares the concentrations of essential and toxic elements in linden blossom collected from disparate locations in Plovdiv, Bulgaria, with those of samples gathered in ecologically pristine regions and commercially available products labeled “bio”. Both total element content and extractable forms in infusions were quantified by ICP-MS. The health risk due to consuming infusions was assessed by comparing the water, tea, drinks, and EFSA regulations. The applied cluster analysis divided the samples from the urban area into three groups related to traffic pollution. In spite of the short blooming period, the concentrations of Al, Fe, Pb, V, Cr, Co, Ni, and Cd in the samples from the most polluted areas are increased by a factor of two compared to those from the clean zones. Full article

To investigate the contamination status and analyze the sources of soil toxic metal contamination on the northern slopes of the East Tianshan mountain industrial belt in Xinjiang, northwest China, this study measured the contents of six common toxic metals such as Zn, Cu, Cr, Pb, Hg and As in 82 surface soil (0–20 cm), and using the ground accumulation index, pollution load index, and improved weighted index assessed the contamination characteristics of the soil and using a semi-variance function and APCS-MLR model the identified the potential sources of contamination. The results indicate that the average concentrations of Pb, Hg, and As are significantly higher than the background values in Xinjiang. The average ranking of toxic metal content is as follows: Zn > Cr > Pb > Cu > Hg > as. A single-factor pollution index analysis shows that As and Pb pollution are severe, while Hg and Cu pollution are moderate. The improved weighted index shows that moderate lead pollution accounts for 6.1% and severe lead pollution accounts for 54.88%; 98.88% of arsenic is severely contaminated. The APCS-MLR model identified three main sources of heavy metals: Cu and Cr as industrial production sources, Pb and Zn as transportation and agricultural activity sources, and As, Cr, Cu, Hg, and Zn as natural and mixed pollution sources. This study provides a solid scientific basis for the prevention and control of toxic metal pollution in agricultural soils, thus ensuring food security and sustainable development in the region. Full article

This study evaluates the concentrations of trace elements (TEs) in soils from the rutile deposit area of Akonolinga, Cameroon, and analyzes the associated health risks. A total of 25 samples were analyzed using flame atomic absorption spectrometry (FAAS). The results show that TE concentrations follow the decreasing order Fe, Ti, Zr, Mn, Cr, V, Ba, Zn, Nb, Ni, Pb, Ga, Cu, Co, Y, Br, and Sn. Pollution indices and the Pearson correlation matrix reveal moderate correlations between Fe and several other TEs, indicating a common origin. Enrichment Factors show significant enrichment in Zr, Nb, and Ti, with notable enrichment in Cr and Co at certain sites. Although the Geo-Accumulation Index indicates no direct contamination and the overall ecological risk is low, the Contamination Factor reveals high levels for Cr, Nb, Ti, and Zr. The carcinogenic risk is moderate, while non-carcinogenic risks are high for children and considerable for adults. These research highlight the potential public health impacts in this mining region and provide essential baseline data for future environmental risk management. Full article

The two cryptic grasshopper species of the genus Stenobothrus, S. eurasius and S. hyalosuperficies, demonstrate different acoustic behavior despite a strong similarity in morphology. A hybridization between these species is possible in the contact zone; however, there are little molecular data about the relationships of these species. The analysis of the mtDNA control region (CR) reveals that haplotypes of S. hyalosuperficies have more in common with the more distant Stenobothrus species than with the closely related S. eurasius. In the contact zone, S. eurasius has mt-haplotypes shared with S. hyalosuperficies, which might indicate an introgression of mtDNA from S. hyalosuperficies to the S. eurasius gene pool. We also analyze the structure and evolutionary rate of the mtDNA CR for the Stenobothrus genus and estimate the time of divergence of the species within the genus. The phylogenetic tree of the tribe Stenobothrini reconstructed with either the CR or COI gave the same four groups. The phylogenetic tree of the Stenobothrus genus has a star-like topology with each mtDNA haplotype found in any analyzed species, except S. eurasius, which forms a separate branch. The maximum degree of incomplete lineage sorting can demonstrate either ancestral polymorphism or introgression. Full article

Fragile X syndrome (FXS) is caused by the full mutation in the FMR1 gene on the Xq27.3 chromosome region. It is the most common monogenic cause of autism spectrum disorder (ASD) and inherited intellectual disability (ID). Besides ASD and ID and other symptoms, individuals with FXS may exhibit sleep problems and impairment of circadian rhythm (CR). The Drosophila melanogaster models of FXS, such as dFMR1^B55, represent excellent models for research in the FXS field. During this study, sleep patterns and CR in dFMR1^B55 mutants were analyzed, using a new platform based on continuous high-resolution videography integrated with a highly-customized version of an open-source software. This methodology provides more sensitive results, which could be crucial for all further research in this model of fruit flies. The study revealed that dFMR1^B55 male mutants sleep more and can be considered weak rhythmic flies rather than totally arrhythmic and present a good alternative animal model of genetic disorder, which includes impairment of CR and sleep behavior. The combination of affordable videography and software used in the current study is a significant improvement over previous methods and will enable broader adaptation of such high-resolution behavior monitoring methods. Full article

The control region (CR) regulates the replication and transcription of the mitochondrial genome (mitogenome). Some avian mitogenomes possess two CRs, and the second control region (CR2) may enhance replication and transcription; however, the CR2 in lark mitogenome appears to be undergoing loss and is accompanied by tandem repeats. Here, we characterized six lark mitogenomes from Alaudala cheleensis, Eremophila alpestris, Alauda razae, and Calandrella cinerea and reconstructed the phylogeny of Passerida. Through further comparative analysis among larks, we traced the evolutionary process of CR2. The mitochondrial gene orders were conserved in all published lark mitogenomes, with Cytb-trnT-CR1-trnP-ND6-trnE-remnant CR2 with tandem repeat-trnF-rrnS. Phylogenetic analysis revealed Alaudidae and Panuridae are sister groups at the base of Sylvioidea, and sporadic losses of CR2 may occur in their common ancestor. CR sequence and phylogeny analysis indicated CR2 tandem repeats were generated within CR2, originating in the ancestor of all larks, rather than inherited from CR1. The secondary structure comparison of tandem repeat units within and between species suggested slipped-strand mispairing and DNA turnover as suitable models for explaining the origin and evolution of these repeats. This study reveals the evolutionary process of the CR2 containing tandem repeat in Alaudidae, providing reference for understanding the evolutionary characteristics and dynamics of tandem repeats. Full article