Search Results (73)

Magnetically actuated catheters have attracted increasing attention for minimally invasive interventions because they enable remote, non-contact steering in confined and tortuous anatomical environments. However, integrating magnetic actuation, electroablation capability, and high structural compliance into a single soft catheter remains challenging. Here, we present a coaxially printed magnetically actuated electroablation catheter (MEC). The MEC is fabricated via a coaxial 3D printing process, combining a highly flexible PDMS outer sheath with a continuously deformable eutectic gallium–indium (eGaIn) conductive core, followed by the distal assembly of a magnetic ring and a copper electrode. This structural design preserves intrinsic mechanical flexibility while maintaining stable electrical conductivity under bending deformation. To achieve active catheter steering, an eight-axis electromagnetic actuation system was developed to generate controllable magnetic fields for tip deflection and guidance. The MEC exhibited effective navigation and manipulation in maze traversal and selective navigation within a 3D-printed vascular model. Furthermore, ex vivo porcine liver and in vivo rat liver electroablation experiments verified that the MEC could be magnetically navigated to designated sites for localized electroablation. This work provides a new strategy for precise, minimally invasive ablation of target tissues in confined and difficult-to-access anatomical environments. Full article

Bone grafting remains limited, and the strategies to design even more structurally complex scaffolds—able to reproduce the hierarchical architecture of bone extracellular matrix—are rapidly growing. In this study, we report the fabrication of a hierarchically structured scaffold produced by layering poly(ε-caprolactone)/gelatin (PCL/Gt) or poly(lactic acid)/gelatin (PLA/Gt) electrospun nanofibers via coaxial electrospinning onto 3D-printed poly(lactic acid) (PLA) scaffolds via fused deposition modeling (FDM). After the printing process, PLA disks (10 × 1 mm, 20% infill, ~80% porosity, pore size ~1.57 mm) were coated with core/shell (PCL/Gt, PLA/Gt) fibers to investigate the in vitro interfacial response of osteoblasts in comparison with monocomponent fibrous coatings (PCL, PLA, Gt). SEM and TEM confirmed that core/shell fibers exhibited bead-free morphologies, with a significant reduction in fiber diameter (≈287–316 nm) and higher interfibrillar porosity compared to monocomponent fibers. FTIR and thermogravimetric analyses indicated the presence of hydrogen bonding between the polyester and gelatin, and the absence of residual solvent after deposition. At the same time, water contact angle measurements confirmed an increase in hydrophilic properties from 80–86° to 120° ascribable to the presence of gelatin. Accordingly, in vitro response of human fetal osteoblasts (hFOB 1.19) exhibited an evident improvement in the case of Gt-based fibrous coatings (i.e., PCL/Gt and PLA/Gt) in terms of early adhesion (4–24 h) and metabolic activity from 3 to 21 days, cell spreading into star-shaped morphologies, formation of extracellular matrix, and mineral phase deposition. In more detail, a remarkable increase in alkaline phosphatase activity was observed in Gt-based coaxial coatings from day 7 onward, with the highest values recorded for PLA/Gt. Overall, we demonstrated that the Gt-based coaxial fibrous coating provided a mix of topological and biochemical cues that synergistically promoted key osteoblast activities at the interface, supporting the regeneration of new bone tissue in highly tailored 3D-printed scaffolds, thus suggesting a promising strategy for personalized regenerative medicine. Full article

Dysphagia and age-related oral processing limitations are rising with population aging and the growing burden of neurological diseases. Texture-modified diets remain the most common non-pharmacological intervention, yet conventional pureeing and thickening often yield meals with low visual appeal, variable textures, and diluted nutrient density, which contribute to reduced intake and malnutrition risk. Extrusion-based three-dimensional food printing, especially when combined with gel-derived edible inks, offers a digital route to standardize geometry, portioning, and texture while enabling individualized nutrition and sensory design. In the past three years, the field has progressed from simple single-ingredient pastes to engineered soft-matter systems including emulsion gels, high-internal-phase emulsion gels, Pickering-stabilized gels, bigels, and multi-material constructs enabled by dual and coaxial printing. These advances are underpinned by improved rheological windowing, microstructure engineering, and post-print gelation strategies such as ionic crosslinking, thermal setting, enzymatic bridging, and pH-triggered network formation. Meanwhile, dysphagia-oriented product development has matured from “shape recovery” demonstrations toward clinically relevant texture targets, leveraging the IDDSI tests to anchor swallowability. This review synthesizes the recent literature across materials science, food engineering, and clinical nutrition to connect gel microstructure to extrusion performance, post-processing stability, and oral processing outcomes that are relevant to older adults and dysphagia patients. We propose design principles for gel network selection, phase structuring, and process control that simultaneously satisfy print fidelity and swallowing safety targets. Full article

Rheology plays an important role in the 3D concrete printing technology, because it directly governs the flowability and shape retention of the material, impacting both the printing process and the final quality of the obtained structure. Local raw materials such as Portland cement, washed sand, and tap water were used for the preparation of 3D-printed concrete mixtures. The solid-state polycarboxylate ether with an anti-foaming agent was used as superplasticizer. The Portland cement was partially replaced (by volume) with a natural zeolite additive in amounts ranging from 0% to 9% in 3D-printed concrete mixtures. A rotational rheometer with coaxial cylinders was used in this research for the determination of rheological characteristics of prepared 3D-printed concrete mixtures. The Herschel–Buckley model was used to approximate experimental flow curves and assess rheological parameters such as yield stress, plastic viscosity, and shear-thinning/thickening index. The additional experiments and calculations, such as water bleeding test and evaluation of the carbon footprint of 3D-printed concrete mixtures, were performed in this work. The replacement of Portland cement with natural zeolite additive positively influenced rheological and stability-related properties of 3D-printed concrete mixtures. Natural zeolite additive consistently reduced water bleeding, enhanced yield stress under increasing shear rates, and lowered plastic viscosity, thereby improving flowability and mixture transportation during the 3D printing process. As the shear-thinning/thickening index remained stable (indicating non-thixotropic behavior in most cases), higher amounts of natural zeolite additive introduced slight thixotropy (especially under decreased shear rates). These changes contributed to better shape retention, layer stability, and the ability to print taller and narrower structures without collapse, making natural zeolite additive suitable for use in the optimized processes of 3D concrete printing. A significant decrease in total carbon footprint (from 3% to 19%) was observed in 3D-printed concrete mixtures with an increase in the mentioned amounts of natural zeolite additive, compared to the mixture without this additive. Full article

This paper presents the design, fabrication, and experimental validation of a metal 3D-printed mechanically tunable cavity resonator operating in the hybrid TM–coaxial resonant mode for the broadband characterization of liquid permittivity. The proposed structure was developed based on a cylindrical cavity by incorporating a disc-terminated metallic tuning stub, which enables continuous frequency adjustment from 0.5 GHz to 3.0 GHz while maintaining a maximum unloaded Q-factor of 284 at 1 GHz under air-filled conditions. The tuning mechanism allows for precise frequency selection for characterizing materials exhibiting frequency-dependent permittivity. To demonstrate its sensing capability, the resonator was applied to characterize ethanol–water mixtures, where resonant frequency shifts were correlated with ethanol concentration at representative baseline frequencies of 1.00 GHz, 2.00 GHz, and 2.94 GHz. The sensor achieved frequency/dielectric constant resolutions of 0.39, 1.34, and 4.20 MHz and average concentration errors of 1.25%, 3.73%, and 2.49%, respectively. Moreover, polynomial fitting models enabled the accurate extraction of dielectric constants with an average deviation below 0.5% compared with a commercial dielectric probe system. The combination of frequency tunability, compact geometry, and compatibility with additive manufacturing establishes the proposed cavity resonator as a versatile platform for broadband dielectric spectroscopy, chemical sensing, and liquid characterization. Full article

Personalized nutrition aims to optimize health by addressing interindividual differences in metabolism, microbiota composition, and dietary responses. Modulating the gut microbiota through probiotics, prebiotics, and synbiotics is promising, yet conventional systems such as capsules or fermented foods offer limited control over dosage, release kinetics, and microbial viability. These formats often cause 2–4 log reductions in viable counts during processing and gastrointestinal transit, underscoring the need for advanced delivery technologies. Three-dimensional (3D) food printing enables digital design of edible matrices with programmable geometry and composition to enhance microbial protection and controlled release. Coaxial and gel-in-gel architectures have retained over 90–96% of probiotic cells after printing and 80–85% after simulated digestion. Synbiotic formulations combining probiotics with fructooligosaccharides or whey protein achieve 98–99% survival and stability for 35 days. This review summarizes advances in formulation, encapsulation, and printing strategies, highlighting how 3D food printing uniquely overcomes challenges of viability, release control, and personalized dosage in microbiota-based nutrition. Full article

This paper presents a broadband near-field probe designed for measuring the normal magnetic field ($H_z$) in radio frequency (RF) circuits operating within a frequency range of 2–8 GHz. The proposed probe uses a cost-effective 4-layer printed circuit board (PCB) structure made with an FR-4 substrate. The probe primarily consists of an $H_z$ detection unit, a broadband microstrip balun, and a coaxial-like output. The broadband balun facilitates the conversion from differential to single-ended signals, thereby enhancing the probe’s common-mode rejection capability. This design ensures that the probe achieves both cost efficiency and high broadband measurement performance. Additionally, this work investigates the feasibility of employing microstrip lines as calibration standards for the $H_z$ probe. The probe’s structural parameters and magnetic field response were initially determined through simulations, and the calibration factor was subsequently verified by calibration experiments. In practical measurements, the field distributions above a microstrip line and a low-noise amplifier (LNA) were captured. The measured field distribution of the microstrip line was compared with simulation results to verify the probe’s performance. Meanwhile, the measured field distribution of the LNA was utilized to identify the radiating components within the amplifier. Full article

This study investigates a novel pulsed electromagnetic field (PEMF) device for dynamic testing and structural health monitoring. The research utilises a PEMF generator CD-1501 with a maximum energy capacity of 0.5 kJ and a flat multifilament coil (IC-1) with a 100 mm diameter. Experiments were conducted on a model steel stand with two joint configurations, using steel plates of 4 mm and 8 mm thickness. The device’s efficacy was evaluated through oscillation pattern analysis and spectral characteristics. Results demonstrate the device’s ability to differentiate between joint states, with the 4 mm plate configuration showing a 15% reduction in high-frequency components compared to the 8 mm plate. Fundamental resonant frequencies of 3D-printed specimens were observed near 5100 Hz, with Q-factors ranging between 200 and 300. The study also found that a 10% increase in volumetric porosity led to a 7% downward shift in resonant frequencies. The developed PEMF device, operating at 50–230 V and delivering 1–5 pulses per minute, shows promise for rapid, non-destructive monitoring of structural joints. When combined with the coaxial correlation method, the system demonstrates enhanced sensitivity in detecting structural changes, utilising an electrodynamic actuator (10 Hz to 2000 Hz range). This integrated approach offers a 30% improvement in early-stage degradation detection compared to traditional methods. Full article

Near-field microwave microscopy (NSMM) is a promising technique for the non-destructive, high-resolution imaging of electrical and dielectric properties at the microscale. However, its performance is highly sensitive to the probe-to-sample distance, often requiring extremely close proximity, which limits its practical application in device manufacturing, especially in scenarios involving coatings and packaging. In this study, we propose a distance inversion method based on a dual-port symmetrical microwave probe to improve imaging performance at larger, safer scanning distances. This method utilizes the correlation between probe height and resonant frequency to compensate for distance-induced signal distortions. The experimental results demonstrate that even at a probe–sample distance of 80 µm, clear and distinguishable NSMM images of printed circuit boards (PCBs) can be obtained. The imaging resolution reached 13 µm. The defect structure with dimensions of 130 × 130 µm² on the PCB was successfully identified. The signal-to-noise ratio was significantly enhanced after applying the correction method. This approach not only improves the robustness and flexibility of NSMM in industrial scenarios but also extends its applicability to packaged or coated electronic devices, offering a valuable tool for advanced non-destructive testing. Full article

Three-dimensional bioprinting technology has emerged as a rapidly advancing multidisciplinary field with significant potential for tissue engineering applications. This technology enables the formation of complex tissues and organs by utilizing hydrogels, with or without cells, as scaffolds or structural supports. Among various bioprinting methods, advanced bioprinting using coaxial and triaxial nozzles stands out as a promising technique. Coaxial bioprinting technique simultaneously deposits two material streams through a coaxial nozzle, enabling controlled formation of an outer shell and inner core construct. In contrast, triaxial bioprinting utilizes three material streams namely the outer shell, inner shell and inner core to fabricate more complex constructs. Despite the growing interest in 3D bioprinting, the development of suitable cell-laden bioinks for creating complex tissues remains unclear. To address this gap, a systematic review was conducted using the preferred reporting items for systematic reviews and meta-analyses (PRISMA) flowchart, collecting 1621 papers from various databases, including Web of Science, PUBMED, SCOPUS, and Springer Link. After careful selection, 85 research articles focusing on coaxial and triaxial bioprinting were included in the review. Specifically, 77 research articles concentrated on coaxial bioprinting and 11 focused on triaxial bioprinting, with 3 covering both techniques. The search, conducted between 1 April and 30 September 2023, had no restrictions on publication date, and no meta-analyses were carried out due to the heterogeneity of studies. The primary objective of this review is to assess and identify the most commonly occurring cell-laden bioinks critical for successful advancements in bioprinting technologies. Specifically, the review focuses on delineating the commonly explored bioinks utilized in coaxial and triaxial bioprinting approaches. It focuses on evaluating the inherent merits of these bioinks, systematically comparing them while emphasizing their classifications, essential attributes, properties, and potential limitations within the domain of tissue engineering. Additionally, the review considers the applications of these bioinks, offering comprehensive insights into their efficacy and utility in the field of bioprinting technology. Overall, this review provides a comprehensive overview of some conditions of the relevant hydrogel bioinks used for coaxial and triaxial bioprinting of tissue constructs. Future research directions aimed at advancing the field are also briefly discussed. Full article

Additive manufacturing (AM) has become a key element of Industry 4.0, particularly the extrusion AM (EAM) of thermoplastic materials, which is recognized as the most widely used technology. Fused Filament Fabrication (FFF), however, depends on expensive commercially available filaments, making pellet extruder-based EAM techniques more desirable. Large-format EAM systems could benefit from printing lightweight objects with reduced material use and lower power consumption by utilizing hollow rather than solid extrudates. In this study, a custom extruder head was designed and an EAM system capable of extruding inflatable hollow extrudates from a variety of materials was developed. By integrating a co-axial nozzle-needle system, a thermoplastic shell was extruded while creating a hollow core using pressurized nitrogen gas. This method allows for the production of objects with gradient part density and varied mechanical properties by controlling the inflation of the hollow extrudates. The effects of process parameters— such as extrusion temperature, extrusion speed, and gas pressure were investigated—using poly-lactic acid (PLA) and styrene-ethylene-butylene-styrene (SEBS) pellets. The preliminary tests identified the optimal range of these parameters for consistent hollow extrudates. We then varied the parameters to determine their impact on the dimensions of the extrudates, supported by analyses of microscopic images taken with an optical microscope. Our findings reveal that pressure is the most influential factor affecting extrudate dimensions. In contrast, variations in temperature and extrusion speed had a relatively minor impact, whereas changes in pressure led to significant alterations in the extrudate’s size and shape. Full article

Additive manufacturing (AM) has emerged as one of the core components of the fourth industrial revolution, Industry 4.0. Among others, the extrusion AM (EAM) of thermoplastic materials has been named as the most widely adopted technology. Fused filament fabrication (FFF) relies on the commercial availability of expensive filaments; hence, pellet extruder-based EAM techniques are desired. Large-format EAM systems would benefit from the ability to print lightweight objects with less materials and lower power consumption, which is possible with the use of hollow extrudates rather than solid extrudates to print objects. In this work, we designed a custom extruder head and developed an EAM system that allows the extrusion of inflatable hollow extrudates of a relatively wide material choice. By incorporating a co-axial nozzle–needle system, a thermoplastic shell was extruded while the hollow core was generated by using pressurized nitrogen gas. The ability to print using hollow extrudates with controllable inflation allows us to print objects with gradient part density with different degrees of mechanical properties. In this article, the effect of different process parameters, namely, extrusion temperature, extrusion speed, and gas pressure, were studied using poly-lactic acid (PLA) pellets. Initially, a set of preliminary tests was conducted to identify the maximum and minimum ranges of these parameters that result in consistent hollow extrudates. Finally, the parameters were varied to understand how they affect the core diameter and shell thickness of the hollow extrudates. These findings were supported by analyses of microscopic images taken under an optical microscope. Full article

This study systematically investigates the effects of the coaxial nozzle’s inner nozzle diameter on the strength and gelation of filaments produced via extrusion-based 3D printing with in situ ionic crosslinking. In this system, bioink (sodium alginate solution) was extruded through the outer nozzle, and the ionic crosslinking solution (calcium chloride solution) was extruded through the inner nozzle. The outer nozzle diameter was fixed at 2.16 mm, and the inner nozzle diameter was varied among 1.19, 0.84, and 0.584 mm. The results indicate that, as the inner nozzle diameter decreased, filament strength decreased, and filament gelation became poorer. These findings highlight the importance of optimizing inner nozzle diameter for improved filament strength and gelation in extrusion-based 3D printing with in situ ionic crosslinking. Full article

Three-dimensional (3D) bioprinting has emerged as an important technique for fabricating tissue constructs with precise structural and compositional control. However, developing suitable bioinks with biocompatible crosslinking mechanisms remains a significant challenge. This study investigates extrusion-based bioprinting (EBB) using uniaxial or coaxial nozzles with enzymatic crosslinking (EC) to produce 3D tissue constructs in vitro. Initially, low-molecular-weight dextran-tyramine and hyaluronic acid-tyramine (LMW Dex-TA/HA-TA) bioink prepolymers were evaluated. Enzymatically pre-crosslinking these prepolymers, achieved by the addition of horseradish peroxidase and hydrogen peroxide, produced viscous polymer solutions. However, this approach resulted in inconsistent bioprinting outcomes (uniaxial) due to inhomogeneous crosslinking, leading to irreproducible properties and suboptimal shear recovery behavior of the hydrogel inks. To address these challenges, we explored a one-step coaxial bioprinting system consisting of enzymatically crosslinkable high-molecular-weight hyaluronic acid-tyramine conjugates (HMW HA-TA) mixed with horseradish peroxidase (HRP) in the inner core and a mixture of Pluronic F127 and hydrogen peroxide in the outer shell. This configuration resulted in nearly instantaneous gelation by diffusion of the hydrogen peroxide into the core. Stable hydrogel fibers with desirable properties, including appropriate swelling ratios and controlled degradation rates, were obtained. The optimized bioink and printing parameters included 1.3% w/v HMW HA-TA and 5.5 U/mL HRP (bioink, inner core), and 27.5% w/v Pluronic F127 and 0.1% H₂O₂ (sacrificial ink, outer shell). Additionally, optimal pressures for the inner core and outer shell were 45 and 80 kPa, combined with a printing speed of 300 mm/min and a bed temperature of 30 °C. The extruded HMW HA-TA core filaments, containing bovine primary chondrocytes (BPCs) or 3T3 fibroblasts (3T3 Fs), exhibited good cell viabilities and were successfully cultured for up to seven days. This study serves as a proof-of-concept for the one-step generation of core filaments using a rapidly gelling bioink with an enzymatic crosslinking mechanism, and a coaxial bioprinter nozzle system. The results demonstrate significant potential for developing designed, printed, and organized 3D tissue fiber constructs. Full article

Vascularized organs hold potential for various applications, such as organ transplantation, drug screening, and pathological model establishment. Nevertheless, the in vitro construction of such organs encounters many challenges, including the incorporation of intricate vascular networks, the regulation of blood vessel connectivity, and the degree of endothelialization within the inner cavities. Natural polymeric hydrogels, such as gelatin and alginate, have been widely used in three-dimensional (3D) bioprinting since 2005. However, a significant disparity exists between the mechanical properties of the hydrogel materials and those of human soft tissues, necessitating the enhancement of their mechanical properties through modifications or crosslinking. In this study, we aim to enhance the structural stability of gelatin–alginate hydrogels by crosslinking gelatin molecules with oxidized pullulan (i.e., a polysaccharide) and alginate molecules with calcium chloride (CaCl₂). A continuous small-diameter vascular network with an average outer diameter of 1 mm and an endothelialized inner surface is constructed by printing the cell-laden hydrogels as bioinks using a coaxial 3D bioprinter. The findings demonstrate that the single oxidized pullulan crosslinked gelatin and oxidized pullulan/CaCl₂ double-crosslinked gelatin–alginate hydrogels both exhibit a superior structural stability compared to their origins and CaCl₂ solely crosslinked gelatin–alginate hydrogels. Moreover, the innovative gelatin and gelatin–alginate hydrogels, which have excellent biocompatibilities and very low prices compared with other hydrogels, can be used directly for tissue/organ construction, tissue/organ repairment, and cell/drug transportation. Full article