Search Results (258)

Background: Increasing antifungal resistance, poor mucosal retention, and systemic side effects limit the effectiveness of currently available drugs. This study explores a novel topical nanotherapeutic approach for the targeted treatment of vulvovaginal candidiasis (VVC), employing green-synthesized silver nanoparticles (AgNPs) derived from Ascophyllum nodosum (AN) and incorporating ibrexafungerp citrate (IBC) into a liposomal formulation. Methods: AgNPs were biosynthesized using AN extract and characterized. Liposomes were prepared by thin-film hydration, and optimised using Central Composite design and characterized and optimized. Optimised liposomes, co-loaded with IBC and AN-AgNPs, were incorporated into a Carbopol-CMC-based topical gel. Results: FTIR shifts in the –OH (3332.31 cm⁻¹) and carbonyl (1636.87 cm⁻¹) bands with reduced intensity confirmed their involvement in Ag⁺ reduction and nanoparticle surface coordination, while the persistence of the 1015 cm⁻¹ band indicated the role of polysaccharides in capping and stabilizing the AN-AgNP. Characterization of the optimized liposomes (IBCL-11) revealed a particle size of 127.2 nm, a zeta potential of −43.8 mV, and a polydispersity index (PDI) of 0.35. Transmission Electron Microscopy (TEM) confirmed the presence of intact, spherical vesicles, while Differential Scanning Calorimetry (DSC) and X-ray diffraction (XRD) validated the molecular dispersion and amorphous characteristics of the films. In vitro evaluations of the IBC liposomal gel demonstrated a sustained drug release of 72.6% over 24 h, alongside enhanced drug penetration across all skin layers. Antifungal assays highlighted the formulation’s potent efficacy, yielding Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) values below 1 µg/mL. Furthermore, the treatments exhibited strong anti-biofilm properties; at MIC and MBC levels, AN-AgNPs achieved biofilm reductions of 45.27 ± 3.16% and 27.62 ± 2.13%, respectively, whereas IBCL-11 produced reductions of 34.25 ± 2.43% and 16.28 ± 1.72%. Conclusion: Ultimately, this study successfully developed an eco-friendly liposomal formulation co-loaded with AN-AgNPs and IBC, offering a promising and targeted therapeutic approach for the treatment of vulvovaginal candidiasis. Full article

This study developed and characterized a hierarchical co-encapsulation system for the delivery of hydrophobic polyphenols (curcumin or quercetin) and the probiotic Lactobacillus rhamnosus GG (LGG). The system was constructed through the self-assembly of zein to form a hydrophobic core for the polyphenols, followed by complex coacervation with debranched starch and chitosan to form an outer hydrophilic layer for LGG. Structural analyses using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and confocal laser scanning microscopy verified the successful formation of the core–shell structure and the transformation of crystalline polyphenols into an amorphous state. The co-encapsulated particles exhibited high encapsulation efficiency (>95% for both polyphenols and LGG) and encapsulation yield (>80% and >75%, respectively). The encapsulation significantly enhanced the antioxidant capacity of both polyphenols in DPPH and ABTS assays, with co-encapsulation of LGG providing a further enhancement. Moreover, the hierarchical structure effectively protected LGG, markedly improving its survival under simulated gastrointestinal conditions and during storage at 4 °C and −20 °C, with quercetin offering stronger protection than curcumin. These findings demonstrate that this co-encapsulation strategy delivers simultaneous protection to both probiotics and polyphenols, providing a robust approach for improving the stability and functionality of multiple bioactive components. Full article

Background: Oral delivery of chemotherapeutic agents remains challenging due to gastrointestinal degradation, poor intestinal permeability, and extensive first-pass metabolism, which collectively limit bioavailability. Lipid-based drug delivery systems offer a promising strategy to overcome these barriers. This study aimed to develop a freeze-dried, solid-dispersible self-emulsifying drug delivery system (SEDDS) using a water-in-oil-in-water (w/o/w) double emulsion approach for the co-encapsulation of hydrophilic (doxorubicin) and lipophilic (ellipticine) agents to enhance oral delivery. Methods: Double-emulsion SEDDS were prepared via a two-stage emulsification process to enable compartmentalized drug loading within aqueous and oil phases. The formulations were freeze-dried to improve stability and storage. Physicochemical properties were characterized using dynamic light scattering for droplet size and polydispersity index (PDI), zeta potential analysis for colloidal stability, and differential scanning calorimetry for thermal behavior. Drug encapsulation efficiency was determined, and cellular uptake was evaluated in breast cancer cells using fluorescence microscopy. Results: Optimized SEDDS exhibited droplet sizes of 90–347 nm with low PDI values (0.005–0.336), indicating uniform and stable dispersions. Zeta potential values (−10.64 to 2.38 mV) supported colloidal stability, while freeze-dried formulations retained dispersion characteristics upon reconstitution over extended storage. Both drugs demonstrated high encapsulation efficiency (>97%), and thermal analysis confirmed the formation of stable amorphous systems. Fluorescence imaging revealed enhanced intracellular uptake of both agents. Conclusions: This study demonstrates that freeze-dried double-emulsion SEDDS enable efficient co-delivery of hydrophilic and lipophilic drugs, improving stability and cellular uptake. This platform shows strong potential for overcoming key barriers in oral chemotherapy and provides a promising strategy for combination drug delivery. Full article

Nanotextured thin film metallic glasses (TFMGs) have emerged as promising antimicrobial coatings for biomedical applications; however, systematic comparisons across compositionally distinct Ti- and Zr-based systems, as well as their early-stage bactericidal mechanisms, remain limited. Here, we show, for the first time, a comparative, compositionally resolved correlation linking alloy chemistry, nanotexture, and bactericidal mechanisms across polymorphic TFMGs. Three co-sputtered biocompatible coatings (Ti₄₇Fe₄₁Cu₁₂, Zr₇₁Fe₃Al₂₆, and Zr₅₈W₃₁Cu₁₁) were deposited on medical-grade titanium and stainless steel (SS316L) via magnetron co-sputtering, producing uniform amorphous films (190–298 nm) with nanoscale roughness of 1.6 ± 0.05 to 8.1 ± 0.05 nm. Surface wettability spanned hydrophilic (71.1 ± 5.6°) to hydrophobic (106.5 ± 3.5°), modulating bacterial interactions. Antimicrobial performance against Pseudomonas aeruginosa was evaluated using live/dead fluorescence imaging, quantitative image analysis, and electron microscopy after 2–4 h incubation. All coatings reduced bacterial adhesion and viability relative to bare substrates, with Zr₅₈W₃₁Cu₁₁ achieving >60% reduction in surface-associated bacterial coverage. Time-resolved analysis revealed a rapid transition to predominantly non-viable populations on coated surfaces, in contrast to sustained viability on controls. Mechanistically, bactericidal activity arises from the synergistic coupling of nanotopography-induced membrane stress, wettability-governed adhesion energetics, and in situ formation of CuO, Fe₂O₃, WO₃, and ZrO₂ oxides that promote electrostatic interactions and proposed reactive oxygen species generation, driving oxidative membrane damage. These results establish a scalable design framework for TFMGs, while highlighting the need for long-term biofilm and electrochemical validation. Full article

Flavanones retrieved in the leaves of Glycyrrhiza glabra (licorice), specifically glabranin (GLA), pinocembrin (PIN) and licoflavanone (LIC), represent a valuable source of bioactive natural products, although their isolation and handling are often complicated by their structural similarity and unfavorable physical properties. In this work, crystal engineering strategies were explored both to facilitate the selective separation of licorice flavanones and to improve their solid-state characteristics. Co-crystallization was investigated as a tool for the selective recognition of PIN from a GLA-rich chromatographic fraction. Guided by structural considerations and predictive analyses performed using the Co-Crystal Design and Hydrogen Bond Propensity (HBP) tools in CCDC Mercury (within CCDC-Materials), co-crystallization experiments were performed with pyridinic co-formers. 4,4′-Bipyridine (BPY) selectively formed a new co-crystal with PIN, enabling the capture of traces of this flavanone directly from the GLA-rich fraction. In contrast, nicotinic acid (NIC) did not form a co-crystal with PIN, consistently with the predicted preference for NIC self-association. In addition, a co-amorphous system between LIC and BPY was obtained by quench cooling, yielding a fully amorphous solid with improved handling properties compared to the waxy precursor. These results highlight the potential of crystal engineering approaches for the selective separation and solid-state modification of natural flavanones. Full article

Background/Objectives: This investigation aims to assess the potential for repurposing nitazoxanide (NIT) as a treatment for COVID-19. NIT was loaded into terpene-enriched chondrosomes (TECs) to assess its anti-hCoV-19 activity through pulmonary delivery. Methods: NIT-TECs were then fabricated utilizing the ethanol injection method. Using a D-optimal design, the effects of factors on entrapment efficiency (EE%), particle size (PS), and zeta potential (ZP) were determined, and the optimal formulation was selected. Results: The optimum TEC exhibited an EE% of 98.87 ± 0.69, a PS of 129.43 ± 5.43 nm, a polydispersity index (PDI) of 0.433 ± 0.022, and a ZP of −25.99 ± 0.99 mV. The optimum TEC was lyophilized to attain a dry powder. Further, the differential scanning calorimetry test confirmed that NIT was transformed from crystalline to amorphous form inside the optimum TEC. In addition, the mucoadhesion test confirmed the ability of the optimum TECs to adhere to pulmonary tissues. Additionally, NIT binding to the active site of SARS-CoV-2 enzymes was investigated using in silico analysis. When compared to NIT, the aerodynamic characteristics of the lyophilized optimum TECs employing the cascade impactor showed superior residence in the lungs. Conclusions: These findings suggest that loading NIT into TECs enhanced its antiviral activity, as indicated by the in vitro cytotoxicity study. Overall, the results point to NIT-loaded TECs as a potentially effective pulmonary delivery system for COVID-19 treatment. Full article

External stray magnetic fields from permanent magnet synchronous motors (PMSMs) may cause electromagnetic interference to nearby equipment and limit their application in space-constrained systems. To address this issue, this paper investigates the use of laminated Co-based amorphous ribbon shields for stray-field suppression. An efficient equivalent modeling method is proposed for the simulation of such multilayer thin shielding structures, in which the laminated shield is replaced by an equivalent single-layer model while preserving its macroscopic shielding behavior. The method is first assessed in 2-D through comparisons between refined laminated and simplified equivalent models under both linear permeability and nonlinear magnetization-curve descriptions, and is then extended to 3-D PMSM shielding analysis under static and rotating no-load conditions with experimental validation. Results show that the 10-layer amorphous ribbon shield, with a total thickness of 420 μm, achieves a maximum shielding effectiveness of 7.9 dB at a measurement distance of two motor radii. The maximum deviation between simulation and experiment is 7.4%, and the equivalent model reduces computation time by 28% relative to the refined model. This method provides an accurate and efficient approach for the analysis and design of compact low-frequency magnetic shields for PMSMs. Full article

This review summarizes innovative co-formulation strategies for non-marketed dry powder inhalers (DPIs), enabling the simultaneous pulmonary delivery of multiple active pharmaceutical ingredients (APIs). Key approaches include co-amorphous systems (COAMS) and co-crystals, which combine two APIs into a single particle, improving aerodynamic properties, solubility, dissolution, and patient compliance while reducing manufacturing complexity. Core–shell microparticles, produced via spray drying, allow spatial separation and controlled release of APIs, minimizing drug–drug interactions and enabling tailored pharmacokinetics. Co-spray drying of dual APIs can yield particles with superior aerosolization and stability, though examples remain limited. Nanoparticle-based systems offer enhanced lung deposition and cellular uptake but face challenges in device compatibility, scalability, and regulatory approval. Each technology presents unique advantages and limitations regarding manufacturability, dose flexibility, and clinical translation. This review also highlights advances in in vitro toxicity testing, including air–liquid interface cultures, organoids, lung-on-chip models, and precision-cut lung slices, which are increasingly important as alternatives to animal studies. The importance of using an aerosol exposure system for the testing is highlighted. Ultimately, the choice of co-formulation platform should balance scientific innovation with practical considerations of manufacturing and regulatory requirements to maximize therapeutic benefit and commercial viability for future DPI combination products. Full article

Calcium–silicate–hydrate (C-S-H), the primary binding phase governing cement paste cohesion, undergoes progressive physicochemical transformation upon carbonation—a process that critically dictates concrete durability in atmospheric environments. When CO₂ penetrates the porous cement matrix, it triggers a cascade of degradation mechanisms: calcium leaching decalcifies the C-S-H structure, inducing polymerization of silicate chains from dimeric to longer-chain configurations, while concurrent precipitation of calcium carbonate and amorphous silica gel fundamentally reconstitutes the nanoscale architecture. These nanoscale alterations propagate to macroscopic property evolution, manifesting as initial strength and stiffness gains due to pore-filling carbonation products followed by eventual deterioration as the cohesive binding network deteriorates. This review synthesizes current understanding of carbonation-induced structural evolution, examining the coupled influences of environmental parameters—CO₂ concentration, relative humidity, and temperature—alongside C-S-H intrinsic chemistry (Ca/Si ratio, aluminum substitution, and alkali content) on reaction kinetics and material performance. However, significant knowledge gaps persist: predictive models for in-service carbonation rates remain elusive due to the disconnect between idealized laboratory conditions and the heterogeneous, cracked reality of field concrete; the causal linkage between nanoscale C-S-H alteration and macroscale cracking patterns along with physical performance is poorly resolved, and most mechanistic studies rely on synthetic C-S-H, neglecting the compositional complexity of real Portland cement systems. We further propose emerging protection strategies, including surface barrier coatings and low-carbon alternative binders (geopolymers, calcium sulfoaluminate cements, carbon-negative materials such as recycled cement), which demonstrate enhanced carbonation resistance. Future research priorities include developing effective coating barriers for carbonation protection, developing operando characterization techniques for real-time reaction monitoring, deploying machine learning algorithms to bridge atomistic simulations with structural-scale predictions, and establishing long-term field performance databases to validate laboratory-derived degradation models. Full article

This review explores the catalytic conversion of carbon dioxide (CO₂) into glycerol carbonate (GC), positioning this pathway as a sustainable strategy that couples environmental mitigation with the valorization of surplus glycerol from biodiesel production. Glycerol carbonate maintains extensive industrial utility as a green solvent, chemical intermediate, and functional component in polymers, cosmetics, and packaging. Distinct from prior literature, this study specifically evaluates the use of amorphous silica from rice husk ash (RHA) as a sustainable, low-cost support, analyzing the synergistic effect between Nb₂O₅, NiO, and ionic liquids in hybrid catalyst architectures. The review evaluates diverse catalytic frameworks, with a primary focus on heterogeneous systems. Silica-based materials are highlighted, particularly those synthesized from rice husk ash, which is an abundant amorphous silica source. The sol–gel method is identified as a robust route for engineering porous matrices with high surface areas and tunable structural properties. Furthermore, the doping of silica with metal oxides, such as niobium oxide (Nb₂O₅) and nickel oxide (NiO), is discussed as a strategic approach to introduce synergistic acid–base sites and redox properties that facilitate CO₂ activation. The integration of ionic liquids into hybrid systems is also examined as a promising frontier to enhance reaction kinetics and selectivity. Finally, this review delineates the nexus between agro-industrial waste management and the reduction in greenhouse gas emissions, proposing a circular economy framework for the biodiesel value chain. Full article

In this study, a novel layered WO₃@Co₃O₄ composite electrode was synthesized via a controlled electrodeposition method employing different surfactants to finely tune its nanostructure. The incorporation of polyacrylic acid (PAA) surfactant yielded an optimized P-W@Co electrode with a hierarchical porous morphology and reduced crystallite size, markedly enhancing electroactive site exposure and electron transport. Structural analyses confirmed the amorphous nature of WO₃ and crystalline spinel Co₃O₄ phases forming an integrated composite architecture. Electrochemical characterizations in a three-electrode system revealed that the P-W@Co electrode exhibited superior pseudocapacitive behavior, with an areal capacitance of 11.70 F/cm² at 20 mA/cm² and excellent rate capability, retaining 80% capacitance at 40 mA/cm². Kinetic studies demonstrated enhanced diffusion-controlled charge storage attributed to improved ion accessibility and charge transfer kinetics. To evaluate practical feasibility, asymmetric supercapacitor devices incorporating P-W@Co as the positive electrode coupled with activated carbon as the negative electrode were fabricated. This device showcased a widened operational voltage (1.5 V), outstanding areal capacitance (211 mF/cm²), and energy density (0.066 mWh/cm²). Importantly, the device exhibited exceptional cycling stability, retaining 81.8% capacitance after 7000 cycles. This work signifies a major advancement in surfactant-mediated design of WO₃@Co₃O₄ layered electrodes for scalable, high-performance supercapacitor applications, combining structural stability, enhanced conductivity, and multifaceted charge storage mechanisms. Full article

Rising atmospheric CO₂ levels have increased the demand for robust, scalable adsorbents for practical CO₂ capture and separation. Porous organic polymers (POPs) are attractive candidates because their pore architecture and binding site properties can be precisely tuned via building blocks and linkage formation. This review summarizes experimental and computational studies of azo-linked POPs and, more broadly, nitrogen–nitrogen (N–N) linked systems, emphasizing how synthetic routes, building blocks, and framework topology govern CO₂ uptake. We highlight key synthetic strategies and representative systems, including porphyrin–azo networks, and discuss the relatively sparse experimental literature on alternative N–N linked POPs incorporating azoxy and azodioxy motifs. Emphasis is placed on reversible nitroso/azodioxide chemistry as a potential pathway to ordered porous organic materials. Computational studies provide a practical route to connect structure with adsorption behavior in largely amorphous or partially ordered networks. We review hierarchical workflows combining periodic DFT and electrostatic potential properties, grand canonical Monte Carlo (GCMC) simulations, and binding energy calculations to rationalize trends and identify favorable binding environments. Computational findings demonstrate that pore accessibility and stacking models can strongly influence predicted CO₂ adsorption. This review provides guidelines for designing POPs with enhanced CO₂ adsorption, offering an outlook and discussing challenges for future studies. Full article

This study investigates the activation potential of various activators for ferronickel slag (FNS) and the associated phase evolution. First, the existing forms of MgO in FNS were identified by analyzing its phase composition across different particle sizes. Subsequently, FNS was activated using six types of activators—Ca(OH)₂, CaO, NaOH, KOH, Na₂CO₃, and a Ca(OH)₂–gypsum composite—under steam curing at 80 °C for 7 days. The setting time, fluidity, hydration products, and mechanical properties of the activated systems were systematically examined. The results show that finer water-cooled FNS particles contain abundant amorphous phases, including amorphous MgO, which can react with Ca-based activators to form hydrotalcite—a reaction not observed with Na- or K-based activators. Compared with Na- or K-based activators, Ca-containing activators, particularly the Ca(OH)₂–gypsum combination, exhibited superior activation performance. In addition, distinct microstructures were observed: NaOH activation promoted the formation of a yarn ball-like N–A–S–H gel, while KOH activation led to a knotted-fiber-bundle-like K–A–S–H phase, the latter showing potential for enhancing the crack resistance of cement-based materials. These findings provide new insights into the activator-dependent hydration mechanisms of FNS and support its value-added utilization in sustainable construction materials. Full article

In the organic biomineralization of guanine (GUA), amorphous GUA is utilized to enhance its solubility, facilitating its transport for the formation of biominerals, and GUA nanocrystals are employed to protect tissues from ultraviolet damage. These principles of GUA biomineralization inspire us to improve the solubility and photostability of trans-resveratrol (RES) using bio-purines, which limits its bioavailability. Bio-purines, such as GUA, hypoxanthine (HYP), and adenine (ADE), were used as co-formers in the amorphous systems of RES. Amorphous RES-2Purines with a 1:2 molar ratio were prepared via the neat ball-milling method and confirmed by powder X-ray diffraction, Raman spectroscopy, and diffuse reflectance spectroscopy. The stability, dissolution profiles, and photostability of RES-2Purines were comprehensively compared. RES-2Purines show high amorphous-to-crystalline transformation temperatures (>100 °C), confirmed by the differential scanning calorimetry-thermogravimetric analysis. Both RES-2HYP and RES-2ADE show an enhanced RES solubility (about 1.6-fold that of raw RES) in water and the simulated gastric fluid (pH 1.2). RES-2Purines can recrystallize quickly after being dispersed in water, which limits the solubility enhancements of RES-2Purines. RES-2Purines have better photostability than raw RES. Bio-purines are promising co-formers for amorphous systems to enhance the solubility and photostability of poorly water-soluble compounds. Full article

Background/Objectives: Oral surgical procedures in patients at risk of/diagnosed with MRONJ require systemic antibiotic therapy, which can fail to achieve an adequate local drug concentration. This research aims to design mucoadhesive buccal patches (containing erythromycin or the erythromycin–resveratrol combination) tailored to the therapeutic needs of patients at risk of MRONJ undergoing oral surgery. Methods: Erythromycin (ERY) and resveratrol (RSV) were embedded into lipid-based microparticles prepared via hot melt dispersion. The microparticles, recovered in the form of dry powders, were characterized in terms of yield, softening/melting temperature, active(s) content, physical state (amorphous vs. crystalline), and individual and bulk properties. Then, they were loaded into a hydrophilic gel, which was dried, obtaining microparticle-loaded buccal patches. The optimized patches were characterized in terms of uniformity, folding endurance, swelling, mucoadhesion, and oromucosal permeation/retention. Results: The microparticles were efficiently produced via a green approach, resulting in reproducible pharmaceutical powders with high loading efficacy (≈90%), spherical morphology, particle sizes in the range of approximately 106–425 μm, and a softening temperature close to body temperature. The buccal patches were also obtained via a green approach, and were found to be thin, flexible, homogeneous, highly swellable, extremely mucoadhesive, and able to promote ERY and RSV accumulation in the buccal tissue (≈25% and 2% of ERY and RSV, respectively, after 2 h) while avoiding active(s) absorption. Conclusions: The proposed buccal patches are viable candidates for further clinical trials aimed at evaluating both the effectiveness of locoregional antibiotic treatment and the usefulness of the co-administration of RSV and ERY. Full article