Search Results (21)

In this study, a pH-responsive nanophotosensitizer (MT@Ce6) was rationally developed by strategic integration of MIL-101 (Fe)-NH₂ metal–organic framework with tannic acid (TA) and chlorin e6. This nanocomposite exhibits pH-responsive degradation in acidic microenvironments, facilitating Fe³⁺ release and subsequent reduction to Fe²⁺ that catalyzes Fenton reaction-mediated hydroxyl radical (•OH) generation. This cascade reaction shifts reactive oxygen species (ROS) predominance from transient singlet oxygen (¹O₂) to the long-range penetrative •OH, achieving robust biofilm disruption and over 90% eradication of methicillin-resistant Staphylococcus aureus (MRSA) under 660 nm irradiation. In vivo evaluations revealed accelerated wound healing with 95% wound closure within 7 days, while species-selective antibacterial studies demonstrated a 2.3-fold enhanced potency against Gram-positive bacteria due to their unique peptidoglycan-rich cell wall architecture. These findings collectively establish a microenvironment-adaptive nanoplatform for precision antimicrobial interventions, providing a translational strategy to address drug-resistant infections. Full article

Fractures and voids are widely distributed in slope rock masses. These defects promote crack initiation and propagation, ultimately leading to rock mass failure. Investigating their damage evolution mechanisms and strength characteristics is of significant importance for slope hazard prevention. A numerical simulation study of Brazilian splitting tests on disk samples containing prefabricated holes and fractures was conducted using the Finite Element Method with Cohesive Zone Modeling (FEM-CZM) in ABAQUS by embedding zero-thickness cohesive elements within the finite element model. This 2021 study analyzed the effects of fracture angle and length on tensile strength and crack propagation characteristics. The results revealed that when the fracture angle is small, cracks initiate near the fracture and propagate and intersect radially as the load increases, ultimately leading to specimen failure, with the crack coalescence pattern exhibiting local closure. As the fracture angle increases, the initiation location of the crack shifts. With an increase in fracture length, the crack initiation position may transfer to other parts of the fracture or near the hole, and longer fractures may result in more complex coalescence patterns and local closure phenomena. During the tensile and stable failure stages, the load–displacement curves of samples with different fracture angles and lengths exhibit similar trends. However, the fracture angle has a notable impact on the curve during the shear failure stage, while the fracture length significantly affects the peak value of the curve. Furthermore, as displacement increases, the proportion of tensile failure undergoes a process of rapid decline, slow rise, and then rapid decline again before stabilizing, with the fracture angle having a significant influence on the proportion of tensile failure. Lastly, as the fracture angle and length increase, the number of damaged cohesive elements shows an upward trend. This study provides novel perspectives on the tensile behavior of fractured rock masses through the FEM-CZM approach, contributing to a fundamental understanding of the strength characteristics and crack initiation mechanism of rocks under tensile loading conditions. Full article

Current management of Ageratina adenophora, a highly invasive weed, relies on synthetic herbicides with environmental and resistance risks, necessitating eco-friendly alternatives. This study evaluated seven phenyl derivatives for phytotoxic activity against A. adenophora via in vitro bioassays. Methyl 4-hydroxyphenylacetate exhibited potent herbicidal efficacy, achieving 100% mortality in 2-month-old seedlings at 30 mM, 3-month-old seedlings at 100 mM, and wild adult/6-month-old plants at 200 mM within 48 h. At 250 mM, the compound reduced CO₂ assimilation by 113.6% and stomatal conductance by 92.2%, indicating severe photosynthetic and transpirational disruption via oxidative stress-mediated chloroplast degradation and stomatal dysfunction. Hormonal profiling revealed significant declines in IAA-ASP, GA1, TZeatin, and TZR, alongside elevated ABA levels, while GA3 remained stable. These hormonal shifts likely drive stomatal closure and metabolic collapse, culminating in plant death. This study provides the first evidence of methyl 4-hydroxyphenylacetate’s dual-action phytotoxicity—targeting both stomatal regulation and hormonal balance—positioning it as a sustainable biocontrol agent for A. adenophora and potentially other invasive weeds. Full article

Robust industry-university-research (I-U-R) collaborative networks are essential for accelerating innovation in the clean energy industry (CEI). This study employs the exponential random graph model to investigate how the network structural, node, and edge attributes drive the formation of I-U-R collaborative networks in China’s CEI, using a dataset of 5276 I-U-R collaborative patents. Key findings reveal the following: (1) convergence structures and transitive closure differentially drive network formation across the growth and maturity phases; (2) the nodes with strong R&D capabilities consistently drive network formation, though enterprises increasingly exert a negative effect and universities show a weakening positive effect; (3) multi-dimensional proximity displays temporal dynamics—geographic proximity follows an inverted U-curve, social proximity is U-shaped, and technological proximity gradually weakens; (4) node types exhibit heterogeneous moderating effects. Enterprises negatively moderate R&D capability during growth and maturity periods, weakening the technological proximity across all periods, and social and geographic proximity in maturity. Universities positively moderate the R&D capability but show period-specific effects on proximity: weakening social proximity in the sprouting stage, geographic proximity in the growth stage, and shifting their moderation of technological proximity from positive (growth) to negative (maturity). These findings deepen the understanding of how the I-U-R collaborative networks in China’s CEI format, contributing to the collaborative innovation theory through insights into the dynamic roles of node types. Full article

Aims: It was the scope of this study to explore the biomechanical implications of retraction force application point modifications in lingual orthodontics, aiming to mitigate the bowing effect and enhance anchorage stability in the anterior teeth. Methods: Using the FE method on an idealized maxillary model, en masse retraction was simulated using a modified lingual fixed appliance including edgewise lingual brackets, a 0.017″ × 0.025″ mushroom-shaped archwire, and power arms between lateral incisors and canines, with a transpalatal arch (TPA) connecting the first molars. Applying bilateral retraction forces of 1.5 N at twelve positions, initial tooth displacements during space closure were evaluated. Results: Shifting power arms gingivally did not effectively counteract palatal tipping of incisors but reduced posterior and palatal tipping of canines with a power arm length of 11.3 mm preventing posterior tipping. Apically displacing the TPA retraction force increased mesiobuccal rotation while preventing mesial molar tipping for retraction forces applied 12.6 mm from the archwire. Conclusions: Apically shifting retraction forces can mitigate vertical bowing effects in lingual orthodontics, yet it also highlights the challenges in maintaining torque in the anterior teeth. Further research and clinical validation are essential in order to confirm these results, emphasizing the complexity and need for advanced biomechanical strategies in personalized lingual orthodontic treatments. Full article

Reasonable excavation step footage and lining support timing are highly important for improving tunnel construction efficiency and ensuring construction safety. Taking the Huanxian No. 1 Tunnel of the Xi-Yin railway as the basis of this study, a 3D numerical model was established using MIDAS GTS NX290 finite element software. This model was used to investigate the deformation and force characteristics of the tunnel-surrounding rock and support structures under three different excavation footages and four different lining construction timings; the numerical results were then compared with the on-site monitoring results. This research aimed to determine reasonable excavation parameters for the three-bench seven-step excavation method used in shallowly buried loess tunnels. The results revealed positive correlations between the excavation step footage and surface subsidence, crown subsidence, and clearance convergence. An excavation footage of 3 m could balance construction efficiency and safety effectively. Keeping the secondary lining construction time unchanged, the early closure of the initial support was beneficial for reducing the force on the secondary lining. Keeping the early closure time of the initial support unchanged, the early construction of the secondary lining would lead to an increase in the force on the secondary lining. The initial support of the tunnel is recommended to be closed as early as possible, and the construction of the secondary lining should be shifted by 21 m behind the upper step palm surface. By comparing the on-site monitoring data with the numerical simulation results, similar trends were observed, providing reference and guidance for the subsequent construction of large-section tunnels in shallowly buried loess formations. Full article

This case report underscores the importance of utilizing E-VAC (endoscopic vacuum-assisted closure) in the treatment of a perforated duodenal ulcer complicated by the formation of a subphrenic abscess and septic shock. It showcases how E-VAC can effectively mitigate the risk of further complications, such as leakage, bleeding, or rupture, which are more commonly associated with traditional methods like stents, clips, or sutures. As a result, there is a significant reduction in mortality rates. A perforated duodenal ulcer accompanied by abscess formation represents a critical medical condition that demands prompt surgical intervention. The choice of the method for abscess drainage and perforation closure plays a pivotal role in determining the patient’s chances of survival. Notably, in patients with a high ASA (American Association of Anesthesiologists) score of IV-V, the mortality rate following conventional surgical intervention is considerably elevated. The management of perforated duodenal ulcers has evolved from open abdominal surgical procedures, which were associated with high mortality rates and risk of suture repair leakage, to minimally invasive techniques like laparoscopy and ingestible robots. Previously, complications arising from peptic ulcers, such as perforations, leaks, and fistulas, were primarily addressed through surgical and conservative treatments. However, over the past two decades, the medical community has shifted towards employing endoscopic closure techniques, including stents, clips, and E-VAC. E-VAC, in particular, has shown promising outcomes by promoting rapid and consistent healing. This case report presents the clinical scenario of a patient diagnosed with septic shock due to a perforated duodenal ulcer with abscess formation. Following an exploratory laparotomy that confirmed the presence of a subphrenic abscess, three drainage tubes were utilized to evacuate it. Subsequently, E-VAC therapy was initiated, with the kit being replaced three times during the recovery period. The patient exhibited favorable progress, including weight gain, and was ultimately discharged as fully recovered. In the treatment of patients with duodenal perforated ulcers and associated abscess formation, the successful and comprehensive drainage of the abscess, coupled with the closure of the perforation, emerges as a pivotal factor influencing the patient’s healing process. The positive outcomes observed in these patients underscore the efficacy of employing a negative pressure E-VAC kit, resulting in thorough drainage, rapid patient recovery, and low mortality rates. Full article

The activity of β-ureidopropionase, which catalyses the last step in the degradation of uracil, thymine, and analogous antimetabolites, is cooperatively regulated by the substrate and product of the reaction. This involves shifts in the equilibrium of the oligomeric states of the enzyme, but how these are achieved and result in changes in enzyme catalytic competence has yet to be determined. Here, the regulation of human β-ureidopropionase was further explored via site-directed mutagenesis, inhibition studies, and cryo-electron microscopy. The active-site residue E207, as well as H173 and H307 located at the dimer–dimer interface, are shown to play crucial roles in enzyme activation. Dimer association to larger assemblies requires closure of active-site loops, which positions the catalytically crucial E207 stably in the active site. H173 and H307 likely respond to ligand-induced changes in their environment with changes in their protonation states, which fine-tunes the active-site loop stability and the strength of dimer–dimer interfaces and explains the previously observed pH influence on the oligomer equilibrium. The correlation between substrate analogue structure and effect on enzyme assembly suggests that the ability to favourably interact with F205 may distinguish activators from inhibitors. The cryo-EM structure of human β-ureidopropionase assembly obtained at low pH provides first insights into the architecture of its activated state. and validates our current model of the allosteric regulation mechanism. Closed entrance loop conformations and dimer–dimer interfaces are highly conserved between human and fruit fly enzymes. Full article

The upper treeline ecotone is a global and typically climate-dependent phenomenon. Its elevation is usually coupled with the thermal limitations of tree growth. The air temperature rise connected with global warming is assumed as the main cause of treeline upslope shifts in the last century. It has been found that the treeline elevation also correlates with the distance from the coastline and the aridity or continentality of the climate or the mass elevation effect. However, previous and contemporary publications have not explained how the upper treeline position directly couples with climate parameters. Often, this has been restricted by a lack of climate measurements and spatial data. In our study, we obtained data from 339 regional weather stations for 1964–1974 and interpolated them to Altay and Western Sayan using regional DEMs and a specially developed regression model. Moreover, we semiautomatically identified the elevational position of the upper open forest boundary (OFB) (crown closure > 10%) on the slopes of 30 mountains in Altay and Western Sayan in 1960 and 2020. We took into account the slope aspect and edaphic constraints. The obtained data allowed us to undertake a regression analysis of the dependence of the OFB elevation on climatic parameters. As a result, we found that, in the 1960s, at OFB elevations rising from the outer to the inner parts of the study area to approximately 500–700 m, the summer air temperature and precipitation linearly decreased, but the summer sunshine duration increased. In the multiple regression analysis, including the climatic parameters as independent variables and the OFB elevation as a dependent variable, significant relations were found only for the combination of air temperature and sunshine duration. We assume that the OFB elevation is determined not only by the air temperature but also by the direct solar irradiation level, changing with latitude and cloudiness. We also found that the ratio between the OFB elevation on the northern and southern slopes varied with respect to latitude. The spatial analysis of OFB shifts in 1960–2020 revealed significant differences in its value in the central (80–90 m) and outer parts of the study area (110–130 m). We suppose that the OFB advance over the past 60 years has local specificity associated with the peculiarities of the climatic changes (summer temperature rise, precipitation decrease, and sunshine duration increase) in different parts of Altay and Western Sayan. Our results highlight the need to clearly determine climatic parameters when forecasting woody vegetation reactions to future climate changes. Full article

Parental engagement is of interest to teachers, school leaders, researchers, and policy makers as a key driver of pupil outcomes. Existing evidence suggests that parental engagement with learning in the home is most effective, but English schools often prioritise school-based events. However, the move to home-learning due to COVID-19 required parents and teachers to play different roles in relation to learning and in relation to each other. Little is known about how this has affected teachers’ perceptions of parental engagement. This mixed-methods, longitudinal case study examined whether teachers’ perceptions of parental engagement changed during COVID-19. Data was gathered from teachers at one large English primary school using interviews (n = 9) and questionnaires (n = 16). Data from before and after the school closures was compared. Teachers reported that parental engagement had become increasingly digital, flexible, and wellbeing-focussed during the school closures. However, teachers were pessimistic about the likelihood of retaining any benefits and their future plans remained focussed on school-based parental engagement events. Whilst school closures resulted in a temporary positive shift towards partnerships and family-centric parental engagement, teachers now need time and training to embed these changes. Without this, some of the potential benefits of the home-learning period may be lost. Full article

Prosodic phrasing is the segmentation of utterances into prosodic words, phonological phrases (smaller units) and intonational phrases (larger units) based on acoustic cues—pauses, pitch changes and pre-boundary lengthening. The perception of prosodic boundaries is characterized by a positive event-related potential (ERP) component, temporally aligned with phrase boundaries—the Closure Positive Shift (CPS). The role of pre-boundary lengthening in boundary perception is still a matter of debate: while studies on phonological phrase boundaries indicate that all three cues contribute equally, approaches to intonational phrase boundaries highlight the pause as the most powerful cue. Moreover, all studies used explicit boundary recognition tasks, and it is unknown how pre-boundary lengthening works in implicit prosodic processing tasks, characteristic of real-life contexts. In this study, we examined the effects of pre-boundary lengthening (original, short, and long) on the EEG responses to intonational phrase boundaries (CPS effect) in European Portuguese, using an implicit task. Both original and short versions showed equivalent CPS effects, while the long set did not elicit the effect. This suggests that pre-boundary lengthening does not contribute to improved perception of boundaries in intonational phrases (longer units), possibly due to memory and attention-related constraints. Full article

Global warming caused by carbon emissions is an environmental issue of great concern to all sectors. Dynamic monitoring of the spatiotemporal evolution of urban carbon emissions is an important link to achieve the regional “double carbon” goal. Using 14 cities (prefectures) in Hunan Province as an example, based on the data of carbon emissions generated by land use and human production and life, and on the basis of estimating the carbon emissions in Hunan Province from 2000 to 2020 using the carbon emission coefficient method, this paper uses the Exploratory Spatial–Temporal Data Analysis (ESTDA) framework to analyze the dynamic characteristics of the spatiotemporal pattern of carbon emissions in Hunan Province from 2000 to 2020 through the Local Indicators of Spatial Association (LISA) time path, spatiotemporal transition, and the standard deviation ellipse model. The driving mechanism and spatiotemporal heterogeneity of urban carbon emissions were studied by using the geographically and temporally weighted regression model (GTWR). The results showed that: (1) In the last 20 years, the urban carbon emissions of Hunan Province have had a significant positive spatial correlation, and the spatial convergence shows a trend of first increasing and then decreasing. Therefore, priority should be given to this relevance when formulating carbon emission reduction policies in the future. (2) The center of carbon emission has been distributed between 112°15′57″~112°25′43″ E and 27°43′13″~27°49′21″ N, and the center of gravity has shifted to the southwest. The spatial distribution has changed from the “northwest–southeast” pattern to the “north–south” pattern. Cities in western and southern Hunan are the key areas of carbon emission reduction in the future. (3) Based on LISA analysis results, urban carbon emissions of Hunan from 2000 to 2020 have a strong path dependence in spatial distribution, the local spatial structure has strong stability and integration, and the carbon emissions of each city are affected by the neighborhood space. It is necessary to give full play to the synergistic emission reduction effect among regions and avoid the closure of inter-city emission reduction policies. (4) Economic development level and ecological environment have negative impacts on carbon emissions, and the population, industrial structure, technological progress, per capita energy consumption, and land use have a positive impact on carbon emissions. The regression coefficients are heterogeneous in time and space. The actual situation of each region should be fully considered to formulate differentiated emission reduction policies. The research results can provide reference for the green and low-carbon sustainable development of Hunan Province and the formulation of differentiated emission reduction policies, and provide reference for other similar cities in central China. Full article

With the rapid spread of COVID-19 worldwide, governments of all countries declared the closure of educational institutions to control its transmission. As a result, institutions were under pressure to offer online education opportunities so that students could continue their education without interruption. The unintended, hasty and unknown duration of the strategy encountered challenges at all pedagogical levels, especially for students who felt stressed out by this abrupt shift, resulting in the decline of their academic performance. Hence, it is necessary to comprehend the approach that might improve students’ involvement and performance in online learning. In this context, the current study used four models to understand the phenomenon: the Task Technology Fit (TTF), the DeLone and McLean Model of Information Systems Success (DMISM), the Technology-to-Performance Chain model (TPC) and the Technology Acceptance Model (TAM). The data for this study were obtained from 404 university students from the top ten universities of Pakistan. The results analyzed using structural equation modeling (SEM) show that learner characteristics positively predict performance through user satisfaction and task technology fit mediating function. Moreover, learner characteristics were also observed to have a significant positive influence on the academic performance of the students, with the mediating functions of user satisfaction and actual usage of the system. Likewise, perceived learning moderated the relationship between learner characteristics and user satisfaction. This research work provides policymakers with a profound framework that emphasizes how employing online learning technologies can strengthen the academic potential of students. Full article

Peri-prosthetic breast infections pose a risk of severe complications after breast implant surgery. The need to remove the breast implant, control the infection and perform additional surgical procedures are the consequences. Reimplantation of an alloplastic implant is only appropriate after an infection-free interval. In this retrospective cohort study, we investigated the effectiveness of negative pressure wound treatment with instillation and dwell time (NPWTi-d) on peri-prosthetic breast infections in combination with implant removal and antibiotic therapy. Twelve patients treated with NPWTi-d due to breast implant infection were included in the study. The bacterial burden was analyzed using wound swabs before and after NPWTi-d. Additionally, laboratory values were determined before NPWTi-d and immediately before wound closure. A total of 13 peri-prosthetic breast infections in 12 patients were treated using implant removal and NPWTi-d. In 76.9% (n = 10) of the cases, the patients had undergone alloplastic breast reconstruction following cancer-related mastectomy, whereas 23.1% (n = 3) of the patients had undergone breast augmentation for cosmetic reasons. The bacterial burden in the breast pocket decreased statistically significant after implant removal and NPWTi-d. No shift from Gram-positive to Gram-negative bacteria was observed. Inflammatory markers rapidly decreased following treatment. NPWTi-d had a positive impact on the healing process after peri-prosthetic breast infections, leading to a decrease in bacterial burden within the wounds and contributing to uneventful healing. Therefore, secondary reimplantation of breast prostheses might be positively influenced when compared to conventional implant removal and simple secondary closure. Further studies are required to conclusively establish the beneficial long-term effects of using NPWTi-d for the treatment of peri-prosthetic breast infections. Full article

Soil heat flux (G) not only affects the Earth’s surface energy balance but also models of calculating soil evaporation. A better understanding on the effect of timing, soil and vegetation on riparian G helps to improve energy balance closure and G simulation in riparian areas with various woodlands. This paper examined diurnal and seasonal variation patterns of soil heat flux in urban riparian areas, together with its relationship with net radiation (Rn) including midday G/Rn and the hysteresis phenomenon under the mutual influence of the timing, soil wetness and vegetation conditions. Study sites lie in the riparian areas of Shanghai with seven vegetation-covered conditions—grassland (C_H), broadleaf evergreen woodlands with shrubs (C_CO), broadleaf evergreen woodlands (C_CH), broadleaf deciduous woodlands with shrubs (C_UO), broadleaf deciduous woodlands (C_UH), conifer with shrubs (C_MO) and conifer (C_MH). Hourly data of Rn and G on typical days in four seasons starting from 11/2020 to 10/2021 were obtained with automated data-logging sensors. Diurnal variations in soil heat flux were characterized as two patterns depending on leaf area index (LAI)—unimodal curves followed cycles of Rn in woodlands with low LAI (C_CH, C_CO, C_H and C_UO) and sinusoidal ones in woodlands with high LAI (C_MO, C_MH and C_UH). Midday G/Rn was generally no more than 10% with slight variations in most woodlands across the four seasons, but upward trends in the grass and C_UO were observed in the afternoon. They were found significantly correlated with SWC. For sparse-canopied riparian sites, hourly G was found to be significantly correlated with Rn and SWC in summer, whereas, for dense sites, the role of canopy characteristics overwhelmed soil properties. Equations were derived to estimate diurnal G from Rn, SWC and LAI. The G of all riparian sites was subject to hysteresis problems to Rn. Phase shifts ranged from one to eight hours in riparian sites and were positively related with LAI and SWC, mainly accounting for the second diurnal pattern of G. Full article