Search Results (11,607)

Takotsubo cardiomyopathy (TCM) is characterized by contractile impairment of the left ventricular apex and excessive contraction of the base of the heart, resulting in transient cardiac dysfunction. Here, we report a case of an implantable cardioverter defibrillator (ICD) that was activated for ventricular fibrillation (VF) caused by TCM one day after removal of maxillomandibular exostoses. The patient was a 53-year-old female who underwent removal of maxillomandibular exostoses in the mid-palate area, bilateral molars of the maxilla, and bilateral mandibular premolars under general anesthesia. Because the patient had a history of VF, an ICD was implanted. Removal was performed without any problems, but VF occurred on the following day, and the ICD was frequently required. Ultrasound examination suggested contractile impairment of the ventricular apex and excessive contraction of the base. Examinations led to a diagnosis of TCM using the Mayo Clinic diagnostic criteria. VF was resolved by administration of amiodarone hydrochloride. The wound in the oral cavity healed favorably, and the patient was discharged from hospital on day 33 without further occurrence of VF. This case highlights important perioperative risk assessment and anesthesia considerations for oral and maxillofacial surgeons managing medically compromised patients undergoing oral surgery. Full article

To overcome the drawbacks of conventional wedge cut blasting—high peak particle velocity (PPV), low blasthole utilization, and a high proportion of large fragments—this paper proposes a delayed blasting method for wedge cut blasting. By integrating the rock-fracturing process of wedge cut blasting, the mechanisms of rock breaking and vibration reduction are investigated and confirm the method through field tests. The results indicate that the rock breaking process can be divided into two stages, the stage of fracture propagation and the stage of cavity ejection, and a rock breaking criterion for wedge cut delayed blasting is established. Considering differences in the vibration waveforms generated by different types of cut holes, a vibration waveform fitting method for wedge cut delayed blasting is proposed. Furthermore, the generation time of the blast-induced free surface during the rock breaking process is calculated, and a calculation Equation for the optimal delayed time is derived. Field tests in the Qi Jiazhuang tunnel show that, compared with conventional blasting, the proposed delayed blasting method increases blasthole utilization by 23.8%, reduces the large fragment rate by 67.4%, lowers PPV by 53.7%, and increases the dominant vibration frequency by 42.0%. These results significantly improve the wedge cut blasting performance and construction safety. Full article

Background/Objectives: Ovarian cancer is the most lethal gynecologic malignancy, largely due to late diagnosis and the high prevalence of malignant ascites, a hallmark of advanced disease that is difficult to control and contributes to immune suppression and treatment failure. Despite advances in standard care, durable responses are rare. This study investigates a novel immunotherapeutic strategy designed to overcome the suppressed peritoneal microenvironment using an oncolytic vaccinia virus engineered to express a decoy-resistant IL-18 mutein. Methods: We generated a vaccinia virus (vvDD-nsmDR-18) expressing a non-secreted, mature, decoy-resistant IL-18. Viral expression was validated via RT-qPCR and fluorescence microscopy, while cytotoxicity was confirmed using CCK-8 assays. The antitumor efficacy of vvDD-nsmDR-18 was evaluated in the aggressive murine ID8a ovarian cancer model. The underlying mechanisms of action were investigated using flow cytometry and transcriptional profiling. Results: Treatment with vvDD-nsmDR-18 significantly prolonged survival and was associated with reduced abdominal distension consistent with decreased ascites burden. Immune analyses indicated enhanced T cell activation across multiple anatomical compartments, including tumors, peritoneal cavity, and spleens, the latter recently suggested to serve as a reservoir for tumor-reactive T cells. This systemic activation was characterized by increased IFN-γ and perforin expression. In addition, vvDD-nsmDR-18 treatment was associated with expansion of CD39⁺CD103⁺CD8⁺ tumor-reactive T cells and a shift toward a lower PD-1 expression phenotype within this population. Conclusions: These findings demonstrate that nsmDR-18-expressing oncolytic viruses can remodel the immunosuppressive landscape of advanced ovarian cancer, suggesting this approach is a promising candidate for further clinical development. Full article

Objectives: Head and neck tumors have been associated with varying risks for venous thromboembolism (VTE). Through a cross-tumor comparison, we assessed site-specific coagulation-related gene expression changes in head and neck squamous cell carcinoma (HNSCC) compared to squamous cell tumors in the esophagus (ESCCa) and lung (LUSC). Further, we assessed the relationship between clinicopathologic features of HNSCC and coagulome gene expression. Methods: RNA-sequencing data from primary tumor tissues of HNSCCa, ESCCa, and LUSC were obtained from The Cancer Genome Atlas (TCGA). Three previously identified pro-thrombotic genes (F3, SERPINE1, and SERPINB2) were analyzed and, for pan-cancer comparisons, gene expression was Z-standardized and summarized as a composite coagulome score. For HNSCCa-specific analyses, gene expression was compared using log2 RSEM counts, contrasting between HPV status, primary tumor site, tumor stage, grade, and demographic characteristics. Results: HNSCCa demonstrated the highest composite coagulome activation (mean Z-score = 0.29, 95% CI: 0.23–0.35) compared with LUSC and ESCCa (mean Z-scores = −0.27 and −0.16, respectively; p < 0.001). Among 487 HNSCCa tumors, HPV-negative tumors exhibited significantly higher composite coagulome expression than HPV-positive tumors (mean ± SD, 11.25 ± 1.39 vs. 10.14 ± 1.30; p < 0.001). Oral cavity tumors demonstrated the highest coagulome expression, while oropharyngeal tumors were most suppressed. Higher histologic grade was inversely associated with coagulome expression (p < 0.001). Patient age, sex, and race were not significantly associated with coagulome expression. Conclusions: HNSCCa exhibits a tumor-specific pro-thrombotic expression profile with substantial heterogeneity driven by HPV status and primary tumor site. Despite elevated tumor-specific pro-coagulant signaling, these findings reflect tumor-specific pro-thrombotic potential rather than clinical VTE risk in HNSCCa, which likely remains context-dependent and may require additional inflammatory or treatment-related triggers to clinically manifest. Full article

Pegmatites with miarolitic cavities have not previously been reported from the Larsemann Hills, East Antarctica, and their age and origin remain poorly constrained. We report the first geochemical and geochronological data for fluorapatite from a newly discovered pegmatite with miarolitic cavities in the Larsemann Hills. Large Fe-rich fluorapatite crystals (up to 5 cm) contain abundant oriented monazite-(Ce) inclusions and display elevated REE (1397–7966 ppm), relatively high Y (945–4192 ppm), and low Sr (52.2–83.5 ppm). Their trace-element signatures plot within the fields of partial melts, high-grade metamorphic rocks, and evolved fluid-rich magmatic systems. U–Pb dating of fluorapatite yields concordant ages of 519 ± 4 Ma (ID-TIMS) and 521 ± 31 Ma (LA-ICP-MS), indicating crystallization during the D₄ stage of the Pan-African orogeny. The isotopic equilibrium between apatite and monazite inclusions suggests synchronous formation and late-stage fluid overprinting. Combined geological, geochemical, and isotopic evidence shows that the pegmatite formed in situ as a product of anatexis of the Broknes paragneisses and evolved within a volatile-rich magmatic–hydrothermal system. These results provide the first direct age constraints on pegmatites with miarolitic cavities in Antarctica and shed new light on the final stages of East Gondwana assembly. Full article

Spin-exchange relaxation-free (SERF) atomic magnetometers have emerged as highly promising candidates for ultra-weak magnetic field detection, particularly in biomagnetic imaging, owing to their exceptional sensitivity, amenability to miniaturization, and near-room-temperature operation. While current miniaturized magnetometers typically employ laser chips with fixed optical power, the quantitative impact of laser power on critical performance metrics remains to be fully elucidated. This study systematically investigates the influence of laser power on sensitivity, bandwidth, and dynamic range by incorporating considerations of power broadening, saturation absorption, and noise constraints. A miniaturized probe, integrated with an actively controlled vertical-cavity surface-emitting laser (VCSEL), was developed for experimental validation. Theoretical and experimental results consistently demonstrate that as optical power increases, sensitivity exhibits a non-monotonic dependence, whereas both bandwidth and dynamic range manifest a monotonic upward trend, aligning well with theoretical simulations. The optimized sensor achieved a peak sensitivity of 16 fT/√Hz at 300 μW, while the bandwidth and dynamic range reached 230 Hz and ±5.4 nT at 500 μW, respectively. This work establishes a robust theoretical and experimental framework for the comprehensive performance optimization of laser-integrated miniaturized atomic magnetometers. Full article

In computer-aided design/computer-aided manufacturing (CAD/CAM) restorations for severely damaged teeth, the cavity floor or proximal margins may be elevated with composite resin to improve adhesion. This in vitro study investigated how different surface pretreatment methods affect the shear bond strength (SBS) of hybrid CAD/CAM materials to dentin or composite surfaces, simulating clinical situations of composite elevation. Hybrid CAD/CAM samples were bonded to dentin or composite substrates following different surface pretreatment protocols and cemented using a dual-cure adhesive resin cement. The samples were thermocycled and subjected to shear bond strength testing, and failure modes were analyzed. The SBS in the sandblasting (SB)+Dentin group and hydrofluoric acid (HF)+Dentin was significantly higher than that in the SB+Composite and HF+Composite groups (p < 0.05). Untreated+composite and untreated+dentin groups showed significantly lower SBS (p < 0.05). Failure mode analysis revealed a predominance of cohesive failures in the SB+Dentin group, while adhesive failures were more frequently observed in most of the other groups. SB-treated and HF-etched hybrid CAD/CAM materials showed more favorable bonding behavior to dentin than to composite, highlighting that bonding to the elevated composite layer may be less effective than bonding directly to prepared dentin. Full article

Oral squamous cell carcinoma (OSCC) is a prevalent form of head and neck cancer that typically develops on the lip or within the oral cavity. Although there have been advances in early detection and treatment, the prognosis for patients, particularly those with advanced-stage disease, remains poor. Liquid biopsy, particularly through the analysis of cell-free DNA (cfDNA) in plasma and urine, has emerged as a promising tool for non-invasive cancer detection and monitoring. This study assessed cfDNA concentration dynamics in plasma and urine samples from 32 OSCC patients, with 5 undergoing genomic characterization by targeted next-generation sequencing (NGS). CfDNA levels were higher in patients compared to healthy controls and showed transient increases following treatment initiation, likely reflecting tumor cell death, followed by a gradual return to baseline. However, cfDNA concentrations were not significantly associated with tumor stage, recurrence, or progression-free survival. Targeted NGS analysis revealed a heterogeneous mutational landscape, identifying 76 variants across tumor tissue and initial cfDNA, with 30.3% shared between both sources. Recurrent hotspot mutations were detected in several important genes, including TP53, PIK3CA, KRAS, APC, and FBXW7. Urine cfDNA also captured several mutations absent from plasma or tissue, supporting its complementary value. These findings demonstrate that cfDNA analysis can dynamically reflect treatment response and capture tumor heterogeneity in OSCC. While informative, cfDNA quantification alone offers limited prognostic reliability, reinforcing the need for a multidimensional approach that includes genomic and clinical evaluation. Overall, this study supports the potential of liquid biopsy as a real-time, non-invasive tool for molecular monitoring and personalized management of OSCC patients. Full article

Candida albicans (C. albicans) is an opportunistic fungal pathogen and a major cause of nosocomial infections, especially in immunocompromised patients. Conventional diagnostic approaches such as blood culture and biochemical assays are accurate but require multi-step sample processing and prolonged turnaround times, limiting their applicability for rapid clinical screening. In the present study, we developed an electrochemical biosensor based on molecularly imprinted polymer (MIP) technology for the rapid and selective detection of intact C. albicans cells. The MIP layer was electropolymerized onto a screen-printed carbon electrode (SPCE), forming selective recognition cavities complementary to the fungal morphology. Electrochemical characterization and detection were performed using cyclic voltammetry in phosphate-buffered saline (PBS). The system demonstrated a wide linear detection range, enabling reliable quantification of C. albicans across concentrations spanning from 1 to 10⁴ CFU/mL and achieved an ultralow limit of detection (LOD) of 1.30 CFU/mL, demonstrating high sensitivity. High selectivity was confirmed against E. coli, S. aureus, and P. aeruginosa, demonstrating that the imprinted cavities effectively distinguish fungal cells from bacterial contaminants. These findings highlight the promise of MIP-based electrochemical biosensors as a simple, low-cost, and portable alternative for early fungal diagnostics. Full article

(1) Research Highlights: This study provides the first integrated assessment of Scots pine (Pinus sylvestris L.) growing in the urban forests of Karaganda, Kazakhstan, a city consistently ranked among the most air-polluted cities globally. We examined the adaptive phyto-chemical response of needles to extreme technogenic stress and evaluated their dual potential as biological filters and renewable sources of bioactive compounds. (2) Background and Objectives: Urban forests are critical for mitigating air pollution; however, the biochemical responses of trees in heavily industrialized environments remain poorly understood. Karaganda faces severe atmospheric pollution from mining, metallurgy, and energy sectors, with particulate matter (PM) levels exceeding permissible limits by up to 20-fold. This study aimed to evaluate the state of Pinus sylvestris, a key component of local protective plantations, by studying heavy metal accumulation, anatomical localization of secondary metabolites, and the phytochemical profile and biological activity of needle extracts obtained using different extraction techniques. (3) Materials and Methods: Needles were collected from 15 trees across three sites in Karaganda’s industrial green zones. Heavy metal content (Pb, Cd, As, and Hg) was determined using atomic absorption spectroscopy and voltammetry. Anatomical–histochemical analysis localizes major metabolite classes. Liquid extracts were prepared using four methods, percolation (PER), vortex-assisted (VAE), microwave-assisted (MAE), and ultrasound-assisted (UAE) extraction, and analyzed by GC-MS. Antimicrobial activity was tested against S. aureus, B. subtilis, E. coli, and C. albicans using the disk diffusion method. The antioxidant capacity (water- and fat-soluble) was measured amperometrically. Statistical analysis was performed using one-way ANOVA with Tukey’s HSD test (p < 0.05). Results: Despite extreme ambient pollution, heavy metal concentrations remained below pharmacopoeial limits (Pb < 0.1, Cd < 0.05, As < 0.01, Hg < 0.001 mg/kg), indicating effective biofiltration without toxic accumulation. Histochemistry confirmed the active synthesis of protective phenolics, flavonoids, and essential oils in the mesophyll, epidermis, and schizogenic cavities. GC-MS identified 72 compounds in the PER extract, 70 (the VAE), 72 in (MAE), and 46 in (UAE). The PER extract exhibited the highest relative abundance of bioactive terpenoids: α-cadinol (5.24%), α-muurolene (4.32%), and caryo-phyllene (2.20%). UAE extracts exhibited elevated 5-hydroxymethylfurfural (6.90%), indicating degradation. Antimicrobial testing revealed that PER produced the largest inhibition zone against S. aureus (15.0 ± 1.0 mm), significantly exceeding that of the other methods (p < 0.001). PER extract also demonstrated the highest water-soluble antioxidant capacity (3600 ± 0.40 mg quercetin equiv./dm³) and substantial fat-soluble activity (1633 ± 0.23 mg gallic acid equiv./dm³). (4) Conclusions: Pinus sylvestris in Karaganda exhibits remarkable adaptive resilience, maintaining safe heavy metal levels while accumulating a rich repertoire of stress-induced secondary metabolites. Classical percolation optimally preserves this native phytocomplex, yielding extracts with superior antimicrobial and antioxidant properties. These findings support a dual-use model wherein urban pine plantations simultaneously serve as living biofilters and renewable sources of standardized bioactive extracts, a concept with direct implications for circular bioeconomy strategies in industrial regions worldwide. This supports the strategic importance of coniferous plantations for bioremediation and sustainable resource use in industrial regions. Full article

Radicular cysts (RCs) represent the most frequent inflammatory cystic lesions of the jaw, typically arising from non-vital teeth. While standard management via enucleation is well-documented, complex cases involving the anterior maxilla present significant surgical challenges due to their proximity to the nasal cavity floor (NCF) and the maxillary sinus floor (MSF). This report provides a comprehensive revision of a clinical case series involving seven patients (ages 17–50) treated with multimodal surgical and regenerative protocols. The patients were stratified into five distinct anatomical risk groups (A–E) based on the integrity of the bony boundaries and the presence of oronasal communications. The treatment strategies combined meticulous cyst enucleation with advanced regenerative techniques, including platelet-rich fibrin (PRF), allogeneic and xenograft bone substitutes, and local flaps such as the buccal fat pad (BFP). The results across all seven cases demonstrated favorable clinical and radiographic outcomes, with no instances of oronasal fistula formation or recurrence during follow-up periods ranging from 12 months to three years. This report emphasizes the necessity of structured anatomical stratification and multimodal planning to ensure scientific precision and surgical predictability in the management of complex maxillary lesions. The differences between approaches towards the nasal cavity and maxillary sinus have to be highlighted. Further studies with larger cohorts are warranted to evaluate the long-term outcomes of different treatment modalities. Full article

Background: Complex head and neck defects often require simultaneous reconstruction of multiple tissue types. The thoracodorsal artery-based chimeric flap offers the potential to address these requirements through a single vascular pedicle. Methods: A retrospective review of patients who underwent head and neck reconstruction using thoracodorsal chimeric flaps at two institutions (2009–2026) was performed. Flap configurations incorporated combinations of the thoracodorsal artery perforator skin paddle, latissimus dorsi muscle, and serratus anterior muscle. Results: Nineteen patients (mean age 63.2 years) were included. Primary sites were the hypopharynx (42.1%) and oral cavity (36.8%). Flap survival was 100%. Reconstruction-related complications occurred in 47.4% of patients, most commonly pharyngocutaneous fistula or leakage (31.6%), all managed conservatively or with secondary closure. Among survivors, 100% achieved tracheostomy decannulation and oral intake. Conclusions: The thoracodorsal chimeric flap may be a useful option for complex head and neck reconstruction requiring multiple tissue components through a single pedicle. However, the complication rate highlights the challenges inherent in this high-risk population, warranting further prospective validation. Full article

A holmium-doped yttrium aluminum garnet (Ho:YAG) thin disk was experimentally investigated under Q-switching and cavity-dumping operation schemes, pumped by a 1.9 µm laser diode (LD). The laser generated pulses at 2090 nm with energies more than 6 mJ and pulse duration down to 3.8 ns, corresponding to a peak power of 1.6 MW with near-diffraction-limited beam quality. The compact and robust system was used for laser-induced breakdown spectroscopy (LIBS) experiments, demonstrating its practical usability. These results represent, to the best of our knowledge, the first demonstration of a Ho:YAG thin-disk laser providing MW peak power in the nanosecond regime. Full article

Background/Objectives: Chronic wounds are a major source of morbidity in patients with paraplegia, often resulting in repeated treatment, prolonged hospitalization, and reduced quality of life. Reconstruction becomes particularly challenging when a wound arises in a scarred trunk region and is further complicated by deep infection, osteomyelitis, or enteric fistula. We describe the staged management of a complex left flank wound in a paraplegic patient, initially reconstructed with a quadrilateral pinwheel flap and later requiring multidisciplinary salvage for recurrence associated with rib osteomyelitis and a colocutaneous fistula. Methods: A paraplegic man in his 50s presented with a chronic left flank wound after repeated full-thickness skin graft failure and persistent Pseudomonas aeruginosa infection. After wide debridement, the approximately 7 × 7 cm defect was reconstructed with a quadrilateral pinwheel flap composed of four Limberg-style rhomboid fasciocutaneous flaps positioned at the 12, 3, 6, and 9 o’clock orientations, elevated at the level of the deep fascia, and transposed into the central defect, with adjunctive negative-pressure wound therapy (NPWT). Approximately 1 year later, recurrence with rib osteomyelitis required rib resection. During NPWT, feculent drainage led to the diagnosis of a colocutaneous fistula. Subsequent multidisciplinary treatment included fistula tract resection, colonic repair with omental patching, transposition of vascularized omentum into the chest wall cavity to obliterate dead space, continued NPWT, and delayed primary closure. Results: Initial local flap reconstruction achieved wound coverage, and immediate postoperative clinical assessment, including pinprick and refill testing, confirmed satisfactory flap perfusion; however, delayed recurrence developed in association with rib osteomyelitis. After definitive fistula surgery, dead-space management with vascularized omentum, wound conditioning with staged NPWT, and delayed primary closure, the wound healed completely. At 6 months after delayed closure, no recurrence of fistula, osteomyelitis, wound dehiscence, or soft-tissue breakdown was observed, and the patient’s daily comfort and functional independence were improved compared with the preoperative condition. Conclusions: A quadrilateral pinwheel flap may provide an effective tension-dispersing local fasciocutaneous option for selected scarred trunk defects in high-risk patients. However, when chronic wounds are compounded by deep infection and enteric fistula, durable healing depends not on flap design alone but on staged multidisciplinary management incorporating definitive source control, vascularized tissue transfer for dead-space elimination, NPWT, and appropriately timed closure. Full article

This paper presents an ultra-miniaturized and high-quality factor embedded loaded coaxial substrate integrated waveguide (ELCSIW) filter. Integrating a substrate-integrated coaxial resonator with a capacitively loaded air cavity achieves a 99% reduction in size compared to a conventional SIW cavity. Incorporating an air gap in the capacitive loading structure significantly enhances the resonator’s quality factor. A comprehensive analysis of the miniaturization factor and quality factor in relation to cavity structure dimensions is performed. Guidelines for fabricating the highly loaded cavity are presented. To demonstrate the benefits of this technique, a two-pole band-pass filter with a 6.3% bandwidth at 1.1 GHz is designed, fabricated, and measured. The overall footprint of the filter is 10.5 mm × 20.5 mm, which is comparable to 0.07 ${\lambda }_g$× 0.14 ${\lambda }_g$. The measured insertion loss is 0.54 dB, and the upper band is spurious-free up to 7 times the resonant frequency. The exceptional performance and compactness of the loaded coaxial substrate integrated waveguide cavities highlight their immense potential for compact advanced wireless systems. Full article