Search Results (1,507)

Background/Objectives: Neurological impairments in children with Cerebral Palsy (CP) often lead to altered muscle architecture and function, resulting in calf muscle contractures. Orthotic immobilization aims to promote muscle–tendon unit lengthening through sustained stretch but may also induce disuse atrophy. This study investigated whether combining immobilization with daily activity yields different effects on muscle strength and gait function compared with immobilization alone. Methods: Fourteen ambulant children with spastic CP and equinus deformity (8 unilateral, 6 bilateral; mean age 9.93 ± 3.0 years; GMFCS I: 10, GMFCS II: 4) participated in a 12-week randomized controlled trial. Participants were assigned to either continuous immobilization (23 h/day) using a dynamic ankle–foot orthosis or a combined protocol consisting of 14 h/day immobilization and 10 h/day of activity involving ankle mobility and calf muscle activation. Outcomes included isometric muscle strength, joint range of motion, gait parameters, and functional measures (Gait Outcomes Assessment List (GOAL) and the Paediatric Outcome Data Collection Instrument (PODCI)). Data were analyzed using linear mixed models with Bonferroni correction. Results: Significant time effects were observed for the knee angle at initial contact (IC), the ankle angle at IC, maximum dorsiflexion, and maximum dorsiflexion during swing. A significant group × time interaction was found only for hindfoot-tibia angle at IC. Within-group improvements were noted in activities of daily living, body image and self-esteem, and basic mobility. No significant changes were found for muscle strength or for most questionnaire subscales. Conclusions: The findings indicate time-related improvements in gait, with no consistent advantage of the combined intervention. Further studies with larger samples are needed to evaluate potential long-term effects. Full article

Winter feeding represents a significant cost in beef production, requiring efficient strategies that maintain productivity while minimizing environmental impact. Forty-six pregnant cows (620 ± 61 kg BW) were used to evaluate an ad libitum hay-based diet (2.02 Mcal/kg ME; HFOR; n = 23) versus a corn-based diet (2.84 Mcal/kg ME) limit-fed at 1.2% BW (HCON; n = 23) from 50 d pre-calving to 84 d post-calving. Pre- and post-calving, HCON cows consumed less (p < 0.01) dry matter, crude protein, and water than HFOR cows. While CH₄ yield per kg DMI was greater (p < 0.01) for HCON cows, total daily CH₄ emissions and CH₄ per unit of NEm intake were lower (p ≤ 0.03) compared with HFOR cows. Behavioral data showed that HCON cows had fewer (p < 0.01) meals and spent less time eating, but had greater intake per minute. Cow BW differed by treatment over time (p < 0.01), with HCON cows weighing less through early lactation, though no differences were observed from d 84 to weaning. Calf BW remained unaffected (p ≥ 0.76). In conclusion, limit-feeding a corn-based diet improves feed and water use efficiency and reduces enteric CH₄ emissions without compromising calf growth, offering a viable alternative to traditional forage-based wintering systems. Full article

External skeletal fixation (ESF) is a versatile method for fracture management in neonatal calves but confers a significant risk of iatrogenic injury to vital structures. The aim of this ex vivo study was to systematically define safe, hazardous, and unsafe corridors for transcortical pin placement in the distal long bones (radius-ulna, tibia, metacarpus, and metatarsus) of Simmental calves to provide precise anatomical guidance. Six fresh Simmental calf cadavers without orthopaedic problems were included in the study. The forearm and hindlimb were disarticulated from the shoulder and hip joints, respectively. The radius-ulna, tibia, and metacarpal and metatarsal bones were dissected transversely from five anatomical levels. Safe, hazardous, and unsafe corridors were determined in each section. No safe corridor was found in the proximal metaphysis and proximal diaphysis of the radius-ulna. Safe corridors were found on the medial side of the radius-ulna, from the mid-diaphysis to the distal metaphysis. The metacarpal and metatarsal bones showed lateral and medial safe corridors. The tibia provided a continuous medial safe corridor. In conclusion, precise topographic mapping is vital for safe ESF. While anatomically safe corridors are the primary choice for ESF, hazardous corridors are viable alternatives if meticulously utilized. By contrast, unsafe corridors must be strictly avoided to prevent severe iatrogenic trauma. Full article

Background: Muscle function determines overall health and is often impaired in metabolic syndrome and cancer, largely due to oxidative stress and inflammation. Olive mill wastewater (OMWW) is rich in bioactive polyphenols (e.g., hydroxytyrosol and verbascoside) that may hinder these potential pro-sarcopenic mechanisms, representing a potential nutraceutical to limit muscle health decline. Objective: To evaluate the effects of short-term supplementation with an OMWW-derived polyphenol extract (Oliphenolia^®, OMWW-OL) on muscle-related parameters and antioxidant biomarkers in adults at metabolic risk while maintaining dietary habits. Methods: This exploratory, hypothesis-driven secondary analysis was based on a single-arm longitudinal pilot study assessing patients at baseline (T0), after 30 days of supplementation (T1), and 30 days post-discontinuation (T2). Anthropometry, bioelectrical impedance, and biochemical assessments were performed. Results: Supplementation was associated with modest increases in skeletal muscle mass, muscle mass percentage, and wrist, arm, and calf circumferences. Fat mass decreased progressively, while total body water percentage and hydration status improved. Ferritin levels rose at T2, alongside increases in protein thiols (PSH) and Trolox equivalent antioxidant capacity (TEAC), suggesting improved iron status and reduced oxidative stress. Body weight and BMI decreased, as expected in a dietary intervention for metabolic syndrome, while muscle health showed a tendency toward improvement. Conclusions: Although the findings require cautious interpretation, short-term OMWW-OL supplementation was associated with modest but consistent directional changes in muscle-related and metabolic indicators in adults at metabolic risk. The results support hypothesis generation and highlight the need for larger studies to further explore the potential role of OMWW-OL in the context of cancer-associated sarcopenia. Full article

Background: Directional hemispherical reflectance (DHR) is a precise method for evaluating skin reflectance and is widely used in dermatological, photobiological, and cosmetic or pharmaceutical research. Reflectance measurements may support emissivity-related interpretation, particularly in the infrared range, being influenced by chromophore content, epidermal structure, and physiological factors such as hydration, pigmentation, and surface heterogeneity. However, most in vivo studies have focused on limited spectral ranges or selected anatomical sites. This study aimed to assess skin directional hemispherical reflectance across a broad spectral range and to provide an integrated dataset supporting emissivity-related interpretation in the infrared region. Methods: The study included 20 women aged 22–50 years (27 ± 9 years) with Fitzpatrick skin phototypes II–III. Reflectance measurements were performed at 14 anatomical sites using an ET 100 emissometer (1.9–21 µm) and an SOC 410 Solar DHR reflectometer (335–2500 nm). Infrared measurements were conducted at incidence angles of 20° and 60° to assess angular effects. Data were statistically analyzed. Results: The lowest reflectance values were observed within 335–380 nm, 1700–2500 nm, and 1.5–21 µm, whereas the highest reflectance was recorded in the 590–720 nm and 700–1100 nm bands. Reflectance symmetry between body sides was observed. In the infrared range, reflectance decreased with increasing wavelength, while mid- and far-infrared values were more uniform across locations. The highest reflectance values were noted for the thigh, calf (crural region), forearm, and palmar surface of the hand, whereas the lowest values were observed in the neck, abdominal region, and dorsal surface of the hand. Measurements at 60° incidence yielded higher reflectance values than those at 20°, while preserving spatial patterns. Conclusions: Directional hemispherical reflectance provides a robust approach for assessing skin reflectance across a broad spectral range. Reflectance depends on wavelength, anatomical location, and physiological factors, including epidermal thickness, pigmentation, and sebum presence. The integrated analysis of spectral, anatomical, and angular variability may support improved interpretation of skin optical properties and contribute to reference data for biomedical and infrared imaging applications. Full article

Controlled delivery using nanoparticle-based systems has attracted considerable attention; however, achieving cell-type specificity remains a major challenge. To address this issue, we focused on the intrinsic cell tropism of viruses. The hepatocyte tropism of hepatitis B virus (HBV) is mediated by interactions between its large envelope protein (L protein) and host factors, including the sodium taurocholate cotransporting polypeptide (NTCP). In this study, we explored viral-like secretory vesicles (VLSVs) displaying HBV spike proteins as a virus-inspired vesicle platform for hepatocyte targeting. We previously established a method for producing VLSVs from HBV L- and S-expressing HEK293T cells. In the present study, we developed an improved protocol using exosome-depleted fetal calf serum and optimized ultracentrifugation, resulting in VLSVs with comparable particle numbers and sizes but approximately tenfold higher protein content per particle. VLSVs were concentrated using a two-layer sucrose cushion, labeled with DiI, and purified by sucrose density gradient ultracentrifugation. We evaluated DiI uptake in hepatocyte-derived cells (HepG2 and Huh7), non-hepatic cells (MDA-MB231, H1299, HeLa, and Vero), and NTCP-overexpressing HepG2 cells. VLSVs showed preferential uptake in the following order: NTCP-overexpressing HepG2 > HepG2 > Huh7 > non-hepatic cells. Furthermore, removal of the N-terminal Flag tag from the L protein enhanced hepatocyte-associated uptake, suggesting the importance of preserving the native structure of the preS1 domain. While vesicle characterization and mechanistic validation remain to be further investigated, these findings provide a proof-of-concept for a virus-inspired vesicle platform exhibiting preferential uptake in hepatocyte-derived cells. Full article

Continuous estimation of oxygen uptake (VO₂) using wearable inertial sensors offers a practical alternative to laboratory-based metabolic testing but remains challenging due to the indirect relationship between kinematics and physiological demand. This study presents a lightweight two-stage pipeline for simultaneous heel-strike (HS) detection and VO₂ estimation using a single calf-mounted IMU. In Stage 1, an Extreme Learning Machine (ELM) + Random Forest (RF) ensemble achieves the highest HS detection F1-score (0.818) under leave-one-subject-out (LOSO) validation, outperforming a temporal convolutional network (TCN) deep learning baseline (F1 = 0.674), which exhibited higher variability across subjects. In Stage 2, kinematic and gait-derived features from 30 s windows are used to estimate normalized VO₂ via RF and ensemble regression under LOSO cross-validation across 24 participants. The RF model achieves a median R² of 0.687 using predicted HS (Pred-HS) events and 0.679 using ground-truth (GT) annotations, with the ensemble showing similar performance (median R² ≈ 0.675–0.691). No statistically significant difference was observed between GT-HS and Pred-HS conditions (p > 0.05). SHAP analysis identifies accelerometer variability (acc_std) and gyroscope-derived features as dominant predictors, with demographic variables contributing minimally. Overall, the results suggest that VO₂ estimation may be achieved using automatically detected gait events without manual annotation. The proposed pipeline is computationally efficient and indicates feasibility under controlled conditions, subject to further validation. Full article

The reaction of the HS^•/S^•− radical (pKa ~3.4), generated selectively from H₂S by γ-irradiated N₂-flushed aqueous solutions at pH 5, with purine nucleosides (dG or dA), a 10-mer double-stranded oligodeoxynucleotide (ds-ODNs), and calf thymus (ct) DNA was investigated, under various experimental conditions. Concurrent quantification of the four purine 5′,8-cyclo-2′-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-2′-deoxypurines (8-oxo-Pu) by LC-MS/MS analysis using isotopomeric internal standards was achieved. The formation of 8-oxo-Pu is several tens of times larger than cPu. Mechanistic schemes for the formation of the two product groups are proposed. Hydrogen atom abstraction from C5′–H by S^•− produces the cPu via cyclization of the C5′ radical onto C8, forming a new covalent bond, C5′–C8. The unexpected formation of 8-oxo-Pu should be mechanistically more complex. We propose that an S^•− (coupled with H⁺) adds to the base rings, followed by the elimination of HS⁻ to form the corresponding radical cation; subsequent reactions with H₂O and radical disproportionation with another S^•− lead to 8-oxo-Pu. A comparison of S^•− with the available literature data for HO^• reactivity towards ct-DNA in de-oxygenated aqueous solutions is also presented. Before the present findings, cPu lesions were attributed exclusively to HO^• reactivity toward ct-DNA. The reaction of the thiyl radical (HOCH₂CH₂S^•) with ct-DNA was also investigated, yielding results similar to those of S^•− obtained under comparable experimental conditions. Our results contributed to a better understanding of DNA damage induced by reactive sulfur species (RSS), particularly the formation of purine lesions and the relative abundance of cPu versus 8-oxo-Pu. Full article

Background/Objective: Peripheral arterial disease (PAD) is a widespread but underdiagnosed manifestation of systemic atherosclerosis, associated with high morbidity and mortality. Traditional diagnostic methods such as the ankle-brachial index (ABI) have limited sensitivity in certain populations, highlighting the need for reliable blood-based biomarkers. Fatty Acid Binding Protein 3 (FABP3) has emerged as a robust biomarker with diagnostic utility in PAD. To evaluate the diagnostic performance of FABP3 when used in combination with traditional clinical risk factors for PAD in patients presenting to vascular surgery clinics. Methods: A retrospective analysis was conducted on 657 patients presenting to ambulatory vascular surgery clinics at St. Michael’s Hospital. Two logistic regression models were compared: (1) Model A: Included standard clinical risk factors (calf pain, age, smoking, diabetes, hypertension, hypercholesterolemia, coronary artery disease, and signs of chronic limb-threatening ischemia); and (2) Model B: Included the same factors as Model A, plus FABP3 levels. Diagnostic metrics including area under the curve (AUC), sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were assessed. Results: Among 657 patients, 423 had PAD and 234 did not. Model B (FABP3-integrated model) outperformed Model A, with a higher AUC (0.86 vs. 0.82), sensitivity (96% vs. 81%), specificity (84% vs. 67%), PPV (92% vs. 81%), NPV (94% vs. 65%), and diagnostic accuracy (93% vs. 76%). FABP3 also improved detection in asymptomatic PAD patients (84% detected vs. 0%). Conclusions: Integrating FABP3 with standard clinical risk factors significantly improves PAD diagnosis, especially in asymptomatic and borderline cases. These findings support the potential role of FABP3 in routine PAD screening, warranting further prospective studies for validation. Full article

This study evaluated the effects of forage source and method of offering on growth performance, starter feed intake, fecal score, rumen fermentation, and selected blood parameters in preweaned Holstein calves. Forty calves were assigned to four treatments (n = 10/group): straw offered separately from starter feed (A1), starter feed containing 7% straw (A2), alfalfa hay offered separately from starter feed (A3), or starter feed containing 7% chopped alfalfa hay (A4). Calves were monitored from birth to 60 days of age. On day 60, body weight was higher in A3 than in A1 (73.37 ± 7.87 vs. 65.82 ± 6.41 kg; p = 0.030). Average daily gain was higher in A3 than in A1 during days 15–30, higher in A3 and A4 than in A1 during days 30–45, and higher in A2 and A3 than in A1 during days 45–60 (p < 0.05). Starter feed intake differed during weeks 5–7, with higher values mainly observed in the straw-fed groups (p < 0.001). Fecal score was higher in A4 than in A2 in week 2 and higher in A4 than in A1 during weeks 3–6 (p < 0.05). Rumen pH on day 60 was higher in A4 than in A1 and A3 (p = 0.003), whereas volatile fatty acid concentrations did not differ among groups. Serum glucose on day 60 was higher in A3 than in A1 (p = 0.002), and total protein was lower in A1 than in A2, A3, and A4 on day 60 (p < 0.001). These findings suggest that forage source and method of offering may produce different biological responses in preweaned calves. Full article

Background: Plyometric training is widely used to enhance explosive performance in youth athletes, yet limited evidence exists regarding the persistence of neuromuscular and morphological adaptations after training cessation in prepubertal populations. Objective: This study examined the effects of an 8-week plyometric training program on sprint performance, change-of-direction (COD) ability, and muscle size-related changes, and evaluated the retention of these adaptations following a 4-week detraining period in prepubertal basketball players. Methods: Twenty-eight prepubertal boys were allocated to either a plyometric training group or a control group. Sprint performance (5, 10, and 20 m) and COD ability were assessed, and muscle size-related changes were evaluated through measurements of muscle volume and cross-sectional area (CSA) of the thigh and calf. Assessments were conducted at baseline, after the intervention, and following the detraining period. Results: The plyometric group demonstrated significant improvements in sprint and COD performance and increases in muscle volume and CSA of the mid-thigh and calf (p ≤ 0.05). The control group showed modest improvements in sprint performance and muscle volume, likely reflecting normal growth and regular basketball participation. After 4 weeks of detraining, sprint and COD performance and muscle volume in the plyometric group declined to levels comparable with the control group, whereas CSA of the mid-thigh and calf remained elevated. Conclusions: These findings indicate that short-term plyometric training improves explosive performance and induces structural adaptations in prepubertal basketball players, although continued training stimuli appear necessary to maintain most performance-related gains. Full article

The objective of this study was to evaluate the effects of timing of injectable trace mineral (ITM) administration (28 days (d) prior to or at weaning) on performance and health in mixed-sex beef calves (n = 115; 224 ± 40 kg). Calves were randomly assigned to one of the following treatments: (1) no ITM (CON), (2) ITM administered 28 d before weaning (PW), or (3) ITM administration at weaning (WEAN). At weaning, calves were transported to a local auction barn, held overnight, and returned the following day; BW, blood, and hair samples were collected prior to and through the receiving period. Data were analyzed using SAS 9.4. Serum Se increased in PW calves following ITM administration (p < 0.01). Serum Mn increased in PW and WEAN groups (p < 0.01) and PW calves showed increased serum Cu at weaning (p < 0.01). Across treatments, calves experienced 6% shrink following weaning and transport, with recovery of BW and intake occurring within 21 d and 8 d, respectively. Despite improved mineral status, no performance benefits were observed during the receiving period, reflecting adequate baseline mineral status and low-stress management conditions, suggesting that ITMs may have limited benefits in well-managed herds. Full article

Aso limonite, a natural volcanic mineral, has been used as a cattle supplement in the Aso region of Japan; however, its effects have not been well-characterized. This study examined whether Aso limonite supplementation from 105 days prepartum to 90 days postpartum changed rumen-associated bacterial communities and calf growth in the Kumamoto strain of Japanese Brown cattle. Buccal swab-derived bacterial profiles were regarded as proxies of examining rumen-associated bacterial communities. Diversity analysis detected prepartum differences between the control and limonite groups. Primary longitudinal screening further identified rumen-associated, oral-associated and archaea subsets from buccal swab-derived microbiota. Within the rumen-associated subset, Blastomonas and Denitromonas emerged as representative taxa showing interaction-associated signals at day −45 in the limonite group, whereas Adlercreutzia showed different pattern at day −15 in the control group. Additionally, heatmap and network analyses showed that these taxa were found in distinct broader rumen-associated bacterial communities rather than a single common taxon. In parallel, calves born to limonite-fed dams were heavier than those from control dams between days 28 to 84 postpartum. These findings indicate that maternal limonite supplementation was associated with selective changes in rumen-associated bacterial communities together with greater early calf body weight. Full article

State-owned agricultural enterprises face intensifying sustainability pressures, yet conventional Environmental, Social, and Governance (ESG) disclosures often mask site-specific operational risks. This study develops a Measurement–Reporting–Verification (MRV)-oriented sustainability internal audit framework for the General Directorate of Agricultural Enterprises (TIGEM) and pilots it across three enterprises (Ceylanpinar, Gozlu, and Karacabey). Utilizing a design-science approach, the model integrates 88 indicators derived from international frameworks (GRI, B Corp, IRIS+) and EU Green Deal requirements with a qualitative risk taxonomy. The results demonstrate that aggregate sustainability scores can obscure critical “Red Zone” risks. Ceylanpinar’s performance is severely constrained by an energy–water nexus (339.2 GWh irrigation demand vs. 263 mm rainfall), while Karacabey faces significant fossil fuel dependency and animal welfare challenges (9.2% calf mortality). Furthermore, the audit identifies tangible legal exposure in Ceylanpinar through 29 labor-related lawsuits linked to subcontracting. The study concludes that bridging the sustainability implementation gap requires a shift from symbolic disclosure to operationalized internal control. These findings provide a preliminary and context-specific roadmap for internal auditors to enhance institutional resilience against climate exposure and global carbo border adjustments (CBAM) in the agricultural sector. Full article

A study was conducted to evaluate the effect of neutral detergent fiber (NDF) levels of calf starter and weaning time on growth, rumen fermentation characteristics, serum metabolites, and rumen microbial diversity of Holstein calves. A total of 24 newly born male Holstein calves were randomly distributed to four groups in a completely randomized design with a 2 × 2 factorial arrangement of NDF levels (14% and 24%) and weaning time (d 44 and d 54). There was no interaction between starter NDF levels and weaning time for any trait except rumen acetic acid in the immediate post-weaning phase (p = 0.013). Starter NDF levels had no effect on growth, feed intake, and hay intake. Late-weaned calves had greater (p = 0.050) weight gain in the pre-weaning phase whereas, early-weaned calves showed greater weight gain (p = 0.004) and starter intake (p = 0.004) in the post-weaning phase although overall weight gain, and starter and hay intakes were not affected by weaning time. Rumen pH, ammonia nitrogen, and most volatile fatty acids remained unaffected by starter NDF levels and weaning except isobutyric acid which was greater in calves fed 24% NDF starter (p = 0.001) in the immediate post-weaning and isovaleric acid which was greater in early-weaned calves (p = 0.044) at the end of experiment. Serum metabolites were largely affected (p < 0.05) by starter NDF levels and weaning time in the pre-weaning phase only. Alpha diversity of rumen microbes was greater and chaotic in 14% NDF starter group (early- and late-weaned) in the pre-weaning phase which converged in the immediate post-weaning phase and diverged on starter NDF basis at the end of experiment. Microbial ecology at phylum and genus levels composition were greatly driven by starter NDF levels in the pre-weaning phase, by weaning time in the immediate post-weaning phase, and two distinct bifurcated microbial ecologies based on starter NDF levels appeared at the end of experiment. In conclusion, the comparable growth with distinct microbial diversity but largely in favor of 24% NDF starter suggests that calves can be subjected to early weaning with 24% starter NDF levels for smooth transition from liquid to solid feed in Holstein calves. Full article