Search Results (585)

Lymphoma continues to pose a serious challenge to global health, underscoring the urgent need for new therapeutic strategies. Recently, the gut microbiome has been shown to play a potential role in regulating immune responses and influencing cancer progression. However, its molecular mechanisms of action in lymphoma remain poorly understood. This study investigates the antiproliferative and apoptotic activities of gut microbiota-derived metabolites, specifically nisin (N) and urolithin B (UB), individually and in combination 7:3 (5750 μM), against the human lymphoma cell line HKB-11. Comprehensive evaluations were performed using Alamar Blue viability assays, combination index (CI) analyses, reactive oxygen species (ROS) quantification, flow cytometry for apoptosis detection, and advanced bottom-up proteomics analyses. N and UB exhibited potent antiproliferative activity, with the 7:3 combination demonstrating strong synergistic effects (CI < 1), significantly enhancing apoptosis (p < 0.01) and ROS production (p < 0.0001) compared to the untreated control. Proteomics analyses revealed substantial alterations in proteins crucial to ribosomal biogenesis, mitochondrial function, cell cycle control, and apoptosis regulation, including a marked downregulation of ribosomal proteins (RPS27; Log₂FC = −3.47) and UBE2N (Log₂FC = −0.60). These findings highlight the potential of N and UB combinations as a novel and practical therapeutic approach for lymphoma treatment, warranting further in vivo exploration and clinical validation. Full article

This research investigates resonator width optimization for simultaneously enhancing electrical performance and mechanical reliability in wideband RF MEMS filters through systematic evaluation of three configurations: 0% (L1), 60% (L2), and 100% (L3) matching ratios between cap and bottom wafers using Au-Au thermocompression bonding. The study demonstrates that resonator width alignment significantly influences both electromagnetic field coupling and bonding interface integrity. The L3 configuration with complete width matching achieved optimal RF performance, demonstrating 3.34 dB insertion loss across 4.5 GHz bandwidth (25% fractional bandwidth), outperforming L2 (3.56 dB) and L1 (3.10 dB), while providing enhanced electromagnetic wave coupling and minimized contact resistance. Mechanical reliability testing revealed superior bonding strength for the L3 configuration, withstanding up to 7.14 Kgf in shear pull tests, significantly exceeding L1 (4.22 Kgf) and L2 (2.24 Kgf). SEM analysis confirmed uniform bonding interfaces with minimal void formation (~180 nm), while Q-factor measurements showed L3 achieved optimal loaded Q-factor (Q_L = 3.31) suitable for wideband operation. Comprehensive environmental testing, including thermal cycling (−50 °C to +145 °C) and humidity exposure per MIL-STD-810E standards, validated long-term stability across all configurations. This investigation establishes that complete resonator width matching between cap and bottom wafers optimizes both electromagnetic performance and mechanical bonding reliability, providing a validated framework for developing high-performance, reliable RF MEMS devices for next-generation communication, radar, and sensing applications. Full article

In this work, research on liquid-based resistive switching devices is carried out, using bottom printable electrodes fabricated from Silver (Ag) paste and silver nitrate (AgNO₃) solution. The self-crossing I-V curves are observed and repeatedly shown by applying 100 sweep cycles, demonstrating repeatability and stability. This liquid device can be refreshed by adding extra droplets of AgNO₃ so that self-crossing I-V hysteresis with up to 493 dual sweeps can be obtained. The ability to be refreshed by supplying a new liquid solution demonstrates an advantage of liquid-based memristive devices, in comparison to their solid counterparts, where the switching layer is fixed after fabrication. The switching mechanism is attributed to Ag migration in the liquid, which narrows the gap between electrodes, giving rise to the observed phenomenon. The devices further show some synaptic properties including excitatory post-synaptic current (EPSC) and potentiation-depression, presenting opportunities to utilize the devices in mimicking some functions of biological neurons. The simplicity and cost-effectiveness of these devices may advance research into fluidic memristors, in which devices with versatile forms and shapes could be fabricated. Full article

Lymphoma continues to pose a significant global health burden, highlighting the urgent need for novel therapeutic strategies. Recent advances in microbiome research have identified gut-microbiota-derived metabolites, or postbiotics, as promising candidates in cancer therapy. This study investigates the antiproliferative and mechanistic effects of two postbiotics, Nisin (N) and Urolithin B (UB), individually and in combination, against the human lymphoma cell line HKB-11. Moreover, this study evaluated cytotoxic efficacy and underlying molecular pathways using a comprehensive experimental approach, including the Alamar Blue assay, combination index (CI) analysis, flow cytometry, reactive oxygen species (ROS) quantification, and bottom-up proteomics. N and UB displayed notable antiproliferative effects, with IC₅₀ values of 1467 µM and 87.56 µM, respectively. Importantly, their combination at a 4:6 ratio demonstrated strong synergy (CI = 0.09 at IC₉₅), significantly enhancing apoptosis (p ≤ 0.0001) and modulating oxidative stress. Proteomic profiling revealed significant regulation of key proteins related to lipid metabolism, mitochondrial function, cell cycle control, and apoptosis, including upregulation of COX6C (Log₂FC = 2.07) and downregulation of CDK4 (Log₂FC = −1.26). These findings provide mechanistic insights and underscore the translational potential of postbiotics in lymphoma treatment. Further preclinical and clinical investigations are warranted to explore their role in therapeutic regimens. Full article

The Jidong Oilfield No. 2 Underground Gas Storage (UGS), located in an active fault zone in Northern China, is a key facility for ensuring natural gas supply and peak regulation in the Beijing–Tianjin–Hebei region. To evaluate the effectiveness of a combined surface and shallow borehole monitoring system under deep reservoir conditions, a 90-day microseismic monitoring trial was conducted over a full injection cycle using 16 surface stations and 1 shallow borehole station. A total of 35 low-magnitude microseismic events were identified and located using beamforming techniques. Results show that event frequency correlates positively with wellhead pressure variations instead of the injection volume, suggesting that stress perturbations predominantly control microseismic triggering. Events were mainly concentrated near the bottom of injection wells, with an average location error of approximately 87.5 m and generally shallow focal depths, revealing limitations in vertical resolution. To enhance long-term monitoring performance, this study recommends deploying geophones closer to the reservoir, constructing a 3D velocity model, applying AI-based phase picking, expanding array coverage, and developing a microseismic-injection coupling early warning system. These findings provide technical guidance for the design and deployment of long-term monitoring systems for deep reservoir conversions into UGS facilities. Full article

Collar monopile foundation is a new type of offshore wind power foundation. This paper explores the horizontal bearing performance of collar monopile foundation in saturated cohesive soil through a combination of physical experiments and numerical simulations. After analyzing the deformation characteristics of the pile–soil system under horizontal load through static load tests, horizontal cyclic loading tests were conducted at different cycles to study the cumulative deformation law of the collar monopile. Based on a stiffness degradation model for soft clay, a USDFLD subroutine was developed in Fortran and embedded in ABAQUS. Coupled with the Mohr–Coulomb criterion, it was used to simulate the deformation behavior of the collar monopile under horizontal cyclic loading. The numerical model employed the same geometric dimensions and boundary conditions as the physical test, and the simulated cumulative pile–head displacement under 4000 load cycles showed good agreement with the experimental results, thereby verifying the rationality and reliability of the proposed simulation method. Through numerical simulation, the distribution characteristics of bending moment and the shear force of collar monopile foundation were studied, and the influence of pile shaft and collar on the horizontal bearing capacity of collar monopile foundation at different loading stages was analyzed. The results show that as the horizontal load increases, cracks gradually appear at the bottom of the collar and in the surrounding soil. The soil disturbance caused by the sliding and rotation of the collar will gradually increase, leading to plastic failure of the surrounding soil and reducing the bearing capacity. The excess pore water pressure in shallow soil increases rapidly in the early cycle and then gradually decreases with the formation of drainage channels. Deep soil may experience negative pore pressure, indicating the presence of a suction effect. This paper can provide theoretical support for the design optimization and performance evaluation of collar monopile foundations in offshore wind power engineering applications. Full article

Pinus radiata D. Don is planted in South Central Chile on a wide range of sites using genetically improved genotypes for timber production. As drought events are expected to increase with ongoing climatic change, the variability in gas exchange, which could impact growth and water use, needs to be evaluated. In this study, we assessed the genotypic variability of leaf-level light-saturated photosynthesis (A_sat), stomatal conductance (g_s), transpiration (E), intrinsic water use efficiency (iWUE), and Chlorophyll a fluorescence (OJIP-test parameters) among 30 P. radiata genotypes (i.e., full-sib families) from third-cycle parents at age 6 years on three sites in Central Chile. We also evaluated tree height (HT), diameter at breast height (DBH), and stem index volume (VOL). Families were ranked for HT as top-15 and bottom-15. In the OJIP-test parameters we observed differences at the family level for the maximum quantum yield of primary PSII photochemistry (Fv/Fm), the probability that a photon trapped by the PSII reaction center enters the electron transport chain (ψ_Eo), and the potential for energy conservation from photons captured by PSII to the reduction in intersystem electron acceptors (PI_ABS). Fv/Fm, PI_ABS, and ψ_Eo ranged from 0.82 to 0.87, 45 to 95, and 0.57 to 0.64, respectively. Differences among families for growth and not for leaf-level physiology were detected. DBT, H, and VOL were higher in the top-15 families (12.6 cm, 8.4 m, and 0.10 m³, respectively) whereas A_sat, g_s, E, and iWUE were similar in both the top-15 and bottom-15 families (4.0 μmol m⁻² s⁻¹, 0.023 mol m⁻² s⁻¹, 0.36 mmol m⁻² s⁻¹, and 185 μmol mol m⁻² s⁻¹, respectively). However, no family by site interaction was detected for growth and leaf-level physiology. The results of this study suggest that highly improved genotypes of P. radiata have uniformity in leaf-level physiological rates, which could imply uniform water use at the stand-level. The family variation found in PI_ABS suggests that this parameter could be incorporated to select genotypes tolerant to environmentally stressful conditions. Full article

Conventional fabrication of high-efficiency organic solar cells (OSCs) predominantly relies on vacuum-evaporated metal top electrodes such as Ag and Al, which hinder large-scale industrial production. Gallium-based liquid metals (GaLMs), particularly the eutectic gallium–indium alloy (EGaIn), represent promising candidates to conventional vacuum-evaporated metal top electrodes due to their excellent printability and high electrical conductivity. In this study, we fabricated vacuum-free OSCs based on GaLM electrodes (Ga, EGaIn, and Galinstan) and analyzed the device performances. Rigid devices with EGaIn electrodes achieved a champion power conversion efficiency (PCE) of 15.6%. Remarkably, all-solution-processed ultrathin flexible devices employing silver nanowire (AgNW) bottom electrodes in combination with EGaIn top electrodes achieved a PCE of 13.8% while maintaining 83.4% of their initial performance after 100 compression–tension cycles (at 30% strain). This work highlights the potential of GaLMs as cost-effective, scalable, and high-performance top electrodes for next-generation flexible photovoltaic devices, paving the way for their industrial adoption. Full article

Innovative approaches in the Portland cement industry, aligned with circular economy principles, offer a promising solution to reduce the environmental impacts. These methods can initially target the architectural elements with lower structural demands, such as urban furniture and paving, before being applied to areas with higher cement usage. Alkali-activated binders (AABs) made from secondary resources present a sustainable alternative to Portland cement (PC), promoting resource recovery, conservation, and a low-carbon economy. Incinerator bottom ash (IBA), traditionally landfilled, has shown potential as a precursor for AABs due to its aluminosilicate content. Repurposing IBA for urban furniture and paving transforms it into a valuable secondary resource. Accordingly, this is the first study to utilize IBA as the sole precursor for urban furniture or paving applications. Research, including state-of-the-art studies and proof of concept developed in this work, demonstrates that IBA-based AABs can produce cast concrete suitable for non-structural urban elements, meeting the technical, environmental, and ecotoxicological standards. Using IBA in AAB formulations not only reduces the reliance on primary raw materials but also contributes to significant energy savings in binder production and lowers greenhouse gas (GHG) emissions, resulting in a reduced carbon footprint. Furthermore, producing concrete from local residual resources, such as IBA, facilitates the reintegration of municipal waste into the production cycle at its point of origin, fostering a sustainable approach to urban development and supporting the circular economy. Full article

Sub-optimal light environments in controlled agricultural settings often limit the productivity of plants. While LED supplementary lighting has been widely adopted to mitigate light deficiencies, the spatial arrangement of LED light sources remains a critical but under-explored factor affecting plant physiological responses. In this study, we used the affiliation function method to comprehensively analyze the effects of four spatial LED supplementary lighting configurations—top-down lighting (T1), mid-canopy upward lighting (T2), mid-canopy downward lighting (T3), and bottom-up lighting (T4)—on the growth and photosynthetic performance of tomato plants. Our findings reveal that the T1 treatment significantly increased light absorption in the upper and middle leaves, enhanced photosynthetic efficiency, promoted the CO₂ assimilation rate, and elevated the activities of key Calvin cycle enzymes, including ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), fructose-1,6-bisphosphatase (FBPase), transketolase (TK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and fructose-1,6-bisphosphate aldolase (FBA). These changes led to improved carbohydrate metabolism and biomass accumulation. Additionally, the T4 treatment markedly enhanced photosynthetic activity in the lower leaves, increasing sugar metabolism-related enzyme activities, such as sucrose synthase (SS), sucrose phosphate synthase (SPS), acid invertase (AI), and neutral invertase (NI). Consequently, compared with the CK treatment, the T4 treatment significantly increased the accumulation of glucose, fructose, and sucrose, with increases of 47.36%, 27.61%, and 87.21%, respectively. Furthermore, LED supplementation regulated endogenous hormone levels, thereby promoting overall plant growth. This study highlights the importance of the spatial arrangement of LEDs in optimizing light distribution and enhancing plant productivity, providing valuable theoretical and practical insights for improving agricultural practices in controlled environments. Full article

Soil organic carbon (SOC) plays a crucial role in the terrestrial carbon cycle and climate regulation. Quantifying the sensitivity of SOC to climate change is essential for developing effective strategies to address climate change and optimizing agricultural production. This study compares the performance of four machine learning models in assessing SOC, ultimately selecting the optimal Extreme Gradient Boosting model for spatial predictions of surface SOC (0–30 cm) across the country. The results indicate that areas with higher organic carbon density are primarily concentrated in the Tibetan Plateau and northeastern regions. Notably, regions with high uncertainty in predictions correspond to areas of elevated organic carbon density. Average temperature, average precipitation, and the Normalized Difference Vegetation Index were identified as the most influential factors across all models. Based on the predictions from the optimal model and a bottom-up framework, various potential climate change scenarios were considered, allowing for the quantification of SOC sensitivity to climate change. Under scenarios of increased temperatures and decreased precipitation, SOC loss intensified, hindering SOC accumulation. When the average temperature rose by 1.45 °C and precipitation decreased by 14.67%, a loss of 10% in SOC was projected for most regions of China. These findings provide critical insights for the proactive formulation of climate adaptation strategies, soil health preservation, and the maintenance of ecosystem stability. Full article

Profiling floats are important platforms for oceanic profile observations, yet they are prone to positional drift and grounding when deployed in shallow-sea environments. In order to address these issues, an aluminum alloy-based propulsion-enabled station-keeping anchoring system (PESKAS) is designed in this paper. The PESKAS comprises anchor wings, thrusters, a steering connector, support frames, and an upper connection flange, which allows easy installation to the bottom of conventional profiling floats. Three anchor wings, with a cone angle of 40° and a length of 0.12 m, enable the attached profiling float to anchor to the seabed under ocean currents of up to 0.5 m/s when fully penetrating the sediment. Numerical simulation results show that achieving full penetration into clay, clayey silt, and silty sand requires thrust forces of 80–100 N, 100–120 N, and 160 N, respectively. To achieve full sediment penetration, the PESKAS employs a redundant quadruple-thruster configuration (total thrust 200 N) with an effective actuation duration of approximately 1 s. It ascends from the seabed via a thruster-generated upward force during the ascent of the profiling float, effectively avoiding grounding. Over a complete operational cycle (descent and ascent), the PESKAS consumes approximately 0.65–1.84 kJ of energy. Compared to the energy consumption of PROVOR profiling float motors (10.25 kJ) and sensors (8.33 kJ), the additional energy requirement for the PESKAS does not have a significant effect on the endurance of profiling floats. According to the results of the simulation experiment of the PESKAS, the system successfully achieves its design objectives of full penetration into and ascending from sediments. PESKAS is a cost-effective solution for the positional drift and grounding of profiling floats, which enables stable long-term profile observations in shallow-sea environments and has broad application prospects. Full article

Technological advancements have led to the development of various devices designed to monitor training loads and athletic performance. Power meters, particularly in cycling, allow for the precise quantification of power output, which is crucial for managing training loads and evaluating performance improvements. This study evaluates the validity of the Quarq D-Zero power meter for measuring cycling power output by comparing it with two previously validated devices—the Favero Assioma Duo (FAD) and the Hammer Saris H3 (H3)—noting that, although it shares the same measurement location as the SRM (the gold standard), it has not been directly validated against it. Thirty-one trained male cyclists participated in this study, undergoing tests across various power outputs (100–500 W) and three 10-s sprint efforts. The protocol incorporated different cadences (70, 85, and 100 revolutions per minute), randomized in order, and two cycling positions (seated and standing). Significant differences (p < 0.05) in power readings were observed among the three power meters, except during sprint efforts. However, pairwise comparisons revealed no significant differences (p > 0.05) between the FAD and Quarq power meters, except for the 500 W block. Strong to very strong correlations were observed between the FAD and Quarq power meters (r > 0.883, ICC > 0.879). The coefficient of variation (CV) between the FAD and Quarq devices ranged from 0.62% to 4.89%, and from 0.39% to 6.59% between the H3 and Quarq power meters. In conclusion, the Quarq power meter, integrated into the spider of the bicycle’s bottom bracket, provides valid power output measurements in cycling. Full article

The durability of permeable pavement needs to be further studied by accelerated pavement testing (APT). Full-scale APT facilities are commonly associated with a very high initial investment and operational costs. A piece of small-scale accelerated testing equipment, the model mobile load simulator (MMLS), was used to investigate and evaluate the mechanical properties of three types of permeable asphalt pavements, including a 4 cm porous asphalt layer with cement-treated permeable base (4PA-CTPB), 7 cm porous asphalt layer with cement-treated permeable base (7PA-CTPB), and 7 cm porous asphalt layer with cement-treated base (7PA-CTB). Under different conditions of subgrade soil, transverse and longitudinal strains at the bottom of the porous asphalt layer and average rut depth and temperature data were collected. The results indicated that 4PA-CTPB produced the maximum average rut depth but minimum resilient tensile strain. The transverse resilient tensile strain of 7PA-CTPB was significantly higher than the other two structures under both wet and dry conditions. The transverse resilient tensile strain significantly increased with increasing loading cycles with a decreasing rate, which could be affected by both load and temperature. MMLS could be used to explore and evaluate the mechanical properties of permeable asphalt pavement. From the data under dry and wet conditions, it may be better to increase the strength of the subgrade, where a suitable hydraulic conductivity coefficient should be considered. Full article

To ensure a high crop profit in Chinese solar greenhouses (CSGs), it is crucial to effectively manage major environmental variables such as temperature, humidity, and CO₂ concentrations, among others, to mitigate harmful effects on crop growth. The objectives of this study were to assess the spatial, vertical, and temporal variability of major environmental variables in CSGs during summer, and to provide fundamental information that could facilitate the monitoring and control of environmental factors in CSGs. The experiments were conducted in two CSGs: one with crops and another without crops. The measured environmental variables included air temperature, humidity, CO₂ concentration, light intensity, and wind conditions. Significant variations in the spatial, vertical, and temporal distribution of environmental factors were observed in both greenhouses. The results revealed significant diurnal patterns in temperature and humidity, with higher daytime temperatures and lower humidity levels. The greenhouse with crops exhibited warmer bottom layers due to restricted air mobility. CO₂ concentrations peaked at night, aligning with plants’ respiration and photosynthesis cycles, whereas light intensity showed substantial daytime peaks, slightly affected by the presence of crops. The study emphasized the necessity of stratified control of the environment and dynamic management of CO₂. The deployment of a wireless sensor network (WSN) and placement of an error-based sensor ensured precise monitoring, highlighting the importance of continuous data collection and adaptive management for optimal greenhouse conditions. Full article