Critical size skeletal defects resulting from trauma and pathological disorders still remain a major clinical problem worldwide. Bone engineering aims at generating unlimited amounts of viable tissue substitutes by interfacing osteocompetent cells of...
Plastic bag bioreactors are single-use bioreactors, frequently used in solid culture fermentation. This study developed plastic bag bioreactors with more effective aeration conditions and particular connection elements that yield sensors, environment...
Recently, plant bioreactors have flourished into an exciting area of synthetic biology because of their product safety, inexpensive production cost, and easy scale-up. Duckweed is the smallest and fastest-growing aquatic plant, and has advantages inc...
This review discusses bioreactor-based methods for industrial hydrocarbon-containing wastewater treatment using different (e.g., stirred-tank, membrane, packed-bed and fluidized-bed) constructions. Aerobic, anaerobic and hybrid bioreactors are becomi...
To produce innovative biopharmaceuticals, highly flexible, adaptable, robust, and affordable bioprocess platforms for bioreactors are essential. In this article, we describe the development of a large-area microfluidic bioreactor (LM bioreactor) for...
In the field of in vitro liver disease models, decellularised organ scaffolds maintain the original biomechanical and biological properties of the extracellular matrix and are established supports for in vitro cell culture. However, tissue engineerin...
A novel, user-friendly bioreactor for the cultivation of cellularised membranes for tissue engineering has been successfully designed, manufactured, and validated. This bioreactor features a culture vessel and a cover, the latter equipped with one or...
In the last decade, there has been a notable advancement in diverse bioreactor types catering to various applications. However, conventional bioreactors often exhibit bulkiness and high costs, making them less accessible to many researchers and labor...
Microbial factories, including microalgae biofactories, have the enormous potential to produce biochemicals for manufacturing diverse bioproducts. A strategic approach to biofactories is maintaining cultures in bioreactors with sufficient resource in...
The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under dif...
Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major facto...
Biotechnological processes involving the presence of microorganisms are realized by using various types of stirred tanks or laboratory-scale dual-impeller commercial bioreactor. Hydrodynamics and mass transfer rate are crucial parameters describing t...
Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of...
Bioprinting provides a powerful tool for regenerative medicine, as it allows tissue construction with a patient’s specific geometry. However, tissue culture and maturation, commonly supported by dynamic bioreactors, are needed. We designed a workflow...
Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control...
The risk of nitrate contamination became a reality for Fairmont in Minnesota, when water rich in NO3-N exceeded the drinking water standard of 10 mg/L. This was unexpected because this city draws its municipal water from a chain of lakes that are fed...
Background and Objectives: To test the long-term ability of human ovarian cortex cells to develop in unconventional culture conditions. Materials and Methods. Ovarian cortex cells from fetuses aged 23 to 39 weeks gestation were cultured for 90 days i...
Most currently available bioreactors have some defects in the expression, activity, or purification of target protein and peptide molecules, whereas the mucus gland of fish can overcome these defects to become a novel bioreactor for the biopharmaceut...
Rotary drum composters are used to produce high-quality, pathogen-free compost without weed seeds. Insulation is usually applied to small-scale composters to warm up the composted materials and enhance metabolic reactions to produce stable and mature...
Multiple efforts have been made to develop small-diameter tissue engineered vascular grafts using a great variety of bioreactor systems at different steps of processing. Nevertheless, there is still an extensive need for a compact all-in-one system p...
The basic characteristic of batch bioreactors is their inability to inflow or outflow the substances during the fermentation process. This follows in the simple construction and maintenance, which is the significant advantage of batch bioreactors. Un...
High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a metho...
A high retention enzymatic bioreactor was developed by coupling membrane distillation with an enzymatic bioreactor (MD-EMBR) to investigate the degradation of 13 phenolic and 17 non-phenolic trace organic contaminants (TrOCs). TrOCs were effectively...
Fermentation is a crucial bioengineering process, existentially important for modern society. The most commonly used production unit for this process is the batch bioreactor. Its main advantage is unsophisticated construction, which unfortunately res...
In this paper, a mathematical model was developed to describe the dynamic behavior of a bioreactor in which a fermentation process takes place. The analysis took into account the bioreactor temperature controlled by the refrigerant fluid flow through...
This paper presents the dynamic behavior of a nonlinear bioreactor model designed for fermentation processes, subject to temperature variations throughout the day. Ethanol production is presented by analyzing the fermenter’s temperature, which...
Calcific aortic valve disease is the most common valvular heart disease in industrialized countries. Pulsatile pressure, sheer and bending stress promote initiation and progression of aortic valve degeneration. The aim of this work is to establish an...
After myocardial infarction, the implantation of stem cell seeded scaffolds on the ischemic zone represents a promising strategy for restoration of heart function. However, mechanical integrity and functionality of tissue engineered constructs need t...
The use of microalgae as a raw material for biogas production is promising. Macroalgae were mixed with cattle manure, wheat straw, and an inoculant from sewage sludge. Mixing macroalgae with co-substrates increased biogas and methane yield. The resea...
This paper proposes an embedded Internet of Things (IoT) system for bioreactor sensor integration, aimed at optimizing temperature and turbidity control during cell cultivation. Utilizing an ESP32 development board, the system makes advances on previ...
Energy produced from plant residue composting has stimulated great interest in heat recovery and utilization. Composting is an exothermic process often controlled through temperature measurements. However, energy analysis of the overall composting sy...
The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated the response of primary human liver cells to toxic drug exp...
Organoid culture is an emerging and promising 3D culture system by which three-dimensional cell aggregates have been produced from different organs and tissues. This new innovative culture technology preserves parental gene expression, as well as the...
The monitoring of must fermentation in a bioreactor, in which the main physico-chemical parameters are tightly controlled, can provide useful analytical information transferable to winemaking on a larger scale. In this experiment, we followed the gro...
The assessment of human liver stem cells (HLSCs) as cell therapeutics requires scalable, controlled expansion processes. We first focused on defining appropriate process parameters for HLSC expansion such as seeding density, use of antibiotics, optim...
Membrane bioreactor systems can enhance anaerobic lactic acid fermentation by reducing product inhibition, thus increasing productivity. In batch fermentations, the bioconversion of glucose is strongly inhibited in the presence of more than 100 g·L−1...
This study presents the procedure of deriving a compartmental model (CM) based on an analysis obtained from the computational fluid dynamics (CFD) model of a bioreactor. The CM is composed of two parts, a structural (that takes into account the archi...
We previously proposed a microfluidic bioreactor with glass–Si–glass layers to evaluate the effect of the fluid force on platelet (PLT) production and fabricated a three-dimensional (3D) microchannel by combining grayscale photolithography and deep r...
In the bioprocessing industry, real-time monitoring of bioreactors is essential to ensuring product quality and process efficiency. Conventional monitoring methods can satisfy some needs but suffer from calibration drift, limited spatial coverage, an...
Galacto-oligosaccharides (GOS) are prebiotic sugars obtained enzymatically from lactose and used in food industry due to their nutritional advantages or technological properties. Selective mass transport and enzymatic synthesis were integrated and fo...
The use of digital twins in tissue engineering (TE) applications is of paramount importance to reduce the number of in vitro and in vivo tests. To pursue this aim, a novel multimodal bioreactor is developed, combining 3D design with numerical stimula...
The anaerobic membrane bioreactors (AnMBR) were operated at 35 °C (H-AnMBR) and 25 °C (L-AnMBR) for long-term wastewater treatment. Two aerobic forward osmosis membrane bioreactors (FOMBRs) were utilized to treat the effluents of H-AnMBR and...
Process development, cheaper bioreactor cost, and faster fermentation rate can aid in reducing the cost of fermentation. In this article, these ideas were combined in developing a previously introduced textile bioreactor for ethanol production. The b...
Biorefinery systems play a critical role in the transition towards a sustainable bioeconomy, and bioreactors are a key component in these systems. While mechanically stirred reactors have been extensively studied, there is a lack of research on pneum...
Cell culture bioreactors play a paramount role in obtaining successful cell cultures that mature into tissue-like structures [...]
Conventional and advanced biological wastewater treatment systems generate excess sludge, which causes socio-economic and environmental issues. This study investigated the performance of membrane-controlled anoxic-oxic-anoxic (AOA) bioreactors for in...
A membrane bioreactor was developed to counteract the inhibition effect of furfural in ethanol production. Furfural, a major inhibitor in lignocellulosic hydrolyzates, is a highly toxic substance which is formed from pentose sugars released during th...
Aerated accumulation bioreactors represent a promising alternative for the aerobic bioremediation of solid contaminated substrates. However, achieving homogeneous mixing and effective air distribution remains a key design challenge in solid-phase sys...
Tissue engineering technology has been advanced and applied to various applications in the past few years. The presence of a bioreactor is one key factor to the successful development of advanced tissue engineering products. In this work, we develope...
Reactive barriers, such as denitrifying bioreactors, have been identified as a clean-up option for nutrient-laden agriculture runoff. Here we tested a 20 m long, 3.75 m wide and 2.2 m deep woodchip bioreactor receiving tile drainage water from a 5.2...
of 55