Search Results (41)

Biofloc technology (BFT), traditionally centered on feed supplementation and water purification in aquaculture, harbors untapped multifunctional potential as a sustainable resource management platform. This review systematically explores beyond conventional applications. BFT leverages microbial consortia to drive resource recovery, yielding bioactive compounds with antibacterial/antioxidant properties, microbial proteins for efficient feed production, and algae biomass for nutrient recycling and bioenergy. In environmental remediation, its porous microbial aggregates remove microplastics and heavy metals through integrated physical, chemical, and biological mechanisms, addressing critical aquatic pollution challenges. Agri-aquatic integration systems create symbiotic loops where nutrient-rich aquaculture effluents fertilize plant cultures, while plants act as natural filters to stabilize water quality, reducing freshwater dependence and enhancing resource efficiency. Emerging applications, including pigment extraction for ornamental fish and the anaerobic fermentation of biofloc waste into organic amendments, further demonstrate its alignment with circular economy principles. While technical advancements highlight its capacity to balance productivity and ecological stewardship, challenges in large-scale optimization, long-term system stability, and economic viability necessitate interdisciplinary research. By shifting focus to its underexplored functionalities, this review positions BFT as a transformative technology capable of addressing interconnected global challenges in food security, pollution mitigation, and sustainable resource use, offering a scalable framework for the future of aquaculture and beyond. Full article

Although biofloc technology (BFT) currently offers advantages such as improving aquaculture water quality, providing natural bait for cultured animals, and reducing pests and diseases, single BFT systems face technical bottlenecks, including the complex regulation of the carbon–nitrogen ratio, accumulation of suspended substances, and acidification of the bottom sludge. Therefore, constructing a composite system with complementary functions through technology integration, such as with aquaponics, biofilm technology, integrated multi-trophic aquaculture systems (IMTAs), and recirculating aquaculture systems (RASs), has become the key path to breaking through industrialization barriers. This paper systematically reviews the action mechanisms, synergistic effects, and challenges of the four mainstream integration models incorporating BFT, providing theoretical support for the environmental–economic balance of intensive aquaculture. Full article

A 56-day trial was conducted to assess the effects of rice straw (RS) and bamboo flour (BF) on growth performance, water quality, gill histology, and the bacterial community of water and the intestine of mandarin fish (Siniperca chuatsi) in biofloc technology systems. The results showed that mandarin fish in the RS and BF groups had comparable survival rates of 100.00 ± 0.00 and 93.33 ± 3.85%; feed conversion ratios of 1.13 ± 0.02 and 1.40 ± 0.15; and weight gain rates of 112.21 ± 1.56 and 100.92 ± 6.45%, respectively. From days 11 to 56 of the farming period, the BF group was more effective than the RS group in removing total ammonia nitrogen (TAN) and NO₂⁻-N, maintaining TAN levels below 0.24 ± 0.05 mg/L. During the early stage of the experiment, the TAN level in the RS group was higher; however, with the supplementation of a carbon source, it gradually decreased and eventually stabilized at 0.13 ± 0.03 mg/L later in the farming period. The secondary gill lamella in the RS group was curved and showed hyperplasia, and the basal gill lamellae showed an increase in the volume of interlamellar cell mass in the BF group. Genes related to denitrification (narG, napA, nirS, nirK, and nosZ) and anammox showed higher expression levels in the BF group than in the RS group, although the differences were not statistically significant (p > 0.05). The results of 16S rRNA sequencing research showed that both treatment groups’ intestinal and water bacterial communities had comparable levels of richness and diversity. Pseudomonas mosselii was the dominant bacterial species in the water. In the BF group, the dominant intestinal species were Bacillus halodurans and Caldalkalibacillus thermarum, while in the RS group, the dominant species was Plesiomonas shigelloides. In conclusion, rice straw and bamboo flour are applicable in BFT systems for mandarin fish culture, with good growth performance and water quality. The BF group showed higher nitrogen removal efficiency and denitrification gene expression than the RS group. Full article

The rising demand for sustainable aquaculture necessitates innovative solutions to environmental and operational challenges. Biofloc technology (BFT) has emerged as an effective method, leveraging microbial communities to enhance water quality, reduce feed costs, and improve fish health. However, traditional BFT systems are susceptible to water quality fluctuations, demanding precise monitoring and control. This review explores the integration of Artificial Intelligence (AI) and Internet of Things (IoT) technologies in smart BFT systems, highlighting their capacity to automate processes, optimize resource utilization, and boost system performance. IoT devices facilitate real-time monitoring, while AI-driven analytics provide actionable insights for predictive management. We present a comparative analysis of AI models, such as LSTM, Random Forest, and SVM, for various aquaculture prediction tasks, emphasizing the importance of performance metrics like RMSE and MAE. Furthermore, we discuss the environmental and economic impacts, including quantitative case studies on cost reduction and productivity increases. This paper also addresses critical aspects of AI model reliability, interpretability (SHAP/LIME), uncertainty quantification, and failure mode analysis, advocating for robust testing protocols and human-in-the-loop systems. By addressing these challenges and exploring future opportunities, this article underscores the transformative potential of AI and IoT in advancing BFT for sustainable aquaculture practices, offering a pathway to more resilient and efficient food production. Full article

Biofloc technology (BFT) represents a promising approach among sustainable options for the sustainable intensification of shrimp aquaculture, helping to mitigate environmental impacts while maintaining production yields. This study evaluated the effects of stocking density (200, 400, 600, and 800 ind/m³) on the water quality, nitrogen dynamics, and production performance of Penaeus vannamei in BFT systems with limited water exchange (<10%). During an eight-week production-scale trial, water quality exhibited density-dependent deterioration, with TAN and NO₂⁻-N peaks increasing from 0.4 to 2.3 mg/L and 1.0 to 4.2 mg/L, respectively, as density rose from 200 to 800 ind/m³. Concurrently, DO and pH declined significantly from 6.7 to 5.1 mg/L and 7.6 to 7.3, respectively. Production performance revealed critical trade-offs: while yield rose from 3.62 to 9.09 kg/m³, individual growth metrics declined, including harvest body weight (19.14 to 14.12 g), size variation (14.03% to 23.90%), and survival rate (94.6% to 79.8%). Quadratic regression analysis and response surface analysis identified 400~600 ind/m³ as the optimal density range, achieving balanced outcomes: yield (6.74~8.43 kg/m³), harvest body weight (16.72~18.03 g), survival rate (84.0%~93.5%), and feed conversion ratio (1.14~1.22). These findings provide actionable guidelines for optimizing stocking density in commercial BFT systems, highlighting the importance of balancing productivity with environmental sustainability under limited water exchange. Full article

This study aimed to evaluate the dynamics of total carbon (TC), total nitrogen (TN), total phosphorus (TP), and some indicators of environmental sustainability of Nile tilapia reared in a biofloc technology (BFT) system. Nile tilapia fingerlings were cultured in three BFT units of production (4.2 m³ each) at a stocking density of 395 fish/m³. After 70 days, the survival rate was 98.05%, with a final average weight of 20.43 g, and apparent feed conversion of 1.05. Nutrient inputs were from supply water, initial fish biomass, and feed; outputs were measured from the final fish biomass and effluent. TC, TN, and TP concentrations in the water increased linearly over time (p < 0.001) by 1.54, 1.66, and 0.44 mg/L, respectively. Feed contributed over 88% of nutrient inputs, while fish final biomass accounted for over 50% of output. Nutrient retention in fish final biomass was 29.74% (TC), 45.38% (TN), and 46.34% (TP). The system had low eutrophication potential, estimated at 57.39 kg TC, 20.02 kg TN, and 5.70 kg TP per ton of fish. Water use was minimal (0.0074 m³/ton), but energy demand was high (114.59 MJ/kg). The closed system reduces biodiversity risks by preventing fish escape. In conclusion, BFT supports high fish productivity with efficient nutrient use, minimal water use, and limited environmental impact, contributing to environmentally sustainable aquaculture. Full article

Background/Objective: Over an eight-week period, this study assessed the influence of dietary carbohydrate levels on growth, metabolism, and immunity in Pacific white shrimp (Litopenaeus vannamei) raised within a biofloc technology (BFT) system. Methods: Five isonitrogenous and isolipidic diets, spanning carbohydrate levels from 11% to 47%, were evaluated. Results: The results showed that dietary carbohydrate significantly impacted both growth performance and feed utilization. The diet containing 38% carbohydrate yielded the best outcomes, resulting in the highest weight gain, specific growth rate, and an optimal feed conversion ratio in the shrimp. Hepatopancreatic metabolic analysis revealed that the shrimp adapted to diets high in carbohydrates through the upregulation of glycolytic enzymes (PK, PFK) and downregulation of gluconeogenic enzymes (PEPCK, G6Pase). By optimizing the water quality and supplementing microbial nutrition, L. vannamei in the BFT system exhibited enhanced dietary carbohydrate utilization and strengthened innate immunity. Specifically, SOD and CAT activities remained largely unaffected by varying carbohydrate levels. However, excessive carbohydrate intake still induced oxidative stress. The high-sugar group (47%) exhibited a significant increase in hemolymph MDA content (p < 0.05), with corresponding metabolic alterations observed in glucose, triglyceride, and total protein levels. On the basis of the results of this study, the BFT system may mitigate the adverse effects of a high-carbohydrate diet by enhancing lysosomal enzyme activity (e.g., ACP) and increasing total protein levels. Conclusions: These findings suggest that the BFT system enhances shrimp immunity and mitigates the potential adverse effects of imbalanced dietary components. Piecewise regression analysis determined the optimal dietary carbohydrate level for shrimp within the BFT system to be 31.44–31.77%. Full article

To meet the growing demand for sustainable aquaculture, the biofloc technology (BFT) system has emerged as a promising solution, offering high productivity, improved water use efficiency, and enhanced environmental and biosecurity performance. Economic and risk analyses are essential tools for identifying the key technical and economic factors that determine the profitability and long-term sustainability of aquaculture systems. This study aimed to evaluate the economic feasibility and the risk associated with Nile tilapia juvenile production in a BFT system. Economic viability indicators were calculated using real data on capital investment, operational costs, and zootechnical performance from a production cycle. Scenario analyses were conducted to assess the effects of fluctuations in input prices and survival rates on overall economic outcomes. Stochastic simulations were also conducted to determine the probabilities of economic results. The items with the greatest impact on costs were the acquisition of the greenhouse and fingerlings, representing 27.64% of the initial investment and 33.24% of the operating cost, respectively. The BFT system showed a positive net margin and profitability per production cycle, with the exception of the pessimistic scenario. The risk analysis demonstrated that in 87.29% of the simulations resulted in a positive profit. Thus, the production of tilapia juveniles in a BFT system is an economically viable investment. However, its success is contingent upon specific technical and market conditions, underscoring the need for careful management and context-specific planning. Full article

This study investigates the synergistic effects of integrating ozone nanobubbles (generated via a pure oxygen-fed reactor with nanobubble-diffusing air stones) and biofloc technology (BFT) on water quality optimization, pathogenic load reduction, and growth performance enhancement in Pacific white shrimp (Penaeus vannamei) prenursery aquaculture systems. Four treatments were tested: a clear water control (CW), ozonated clear water (CW + O), biofloc (FLOC), and biofloc with ozone (FLOC + O). The FLOC + O group significantly improved water quality, reducing total ammonia nitrogen (TAN) by 61%, nitrite nitrogen (NO₂⁻-N) by 78% compared to CW, and total suspended solids (TSS) by 21% compared to FLOC (p = 0.0015). Ozone application (maintained above 0.3 mg/L, 15 min/day) demonstrated robust pathogen suppression, achieving a sharp reduction in Muscle Necrosis Virus (MNV), a 99.5% inhibition of Vibrio spp. (from 228,885 to 107 CFU/mL), and the clearance of Epistylis spp., as determined via optical microscope. These enhancements directly translated to superior biological outcomes, with the FLOC + O group exhibiting an 82% survival rate (vs. 40% in CW) and 13% higher final body weight (11.65 mg vs. 10.32 mg in CW). The integration of ozone and BFT also accelerated larval development and improved the Zoea II to Mysis I metamorphosis success rate. By maintaining stable microbial communities and reducing organic waste, the combined system lowered the water exchange frequency by 40% and eliminated the need for prophylactic antibiotics. These results demonstrate that ozone–BFT integration effectively addresses key challenges in shrimp prenursery—enhancing disease resistance, optimizing water conditions, and improving growth efficiency. The technology offers a sustainable strategy for the intensive prenursery of Pacific white shrimp, balancing ecological resilience with production scalability. Full article

In response to the growing market demand for Litopenaeus vannamei, a variety of single-species, high-density, intensive, and high-yield aquaculture modes have arisen. These aquacultural systems are teeming with microorganisms, which play roles in water quality and host health. To uncover the prokaryotic community composition across cultivation modes, we investigated the prokaryotic community composition at two fractionated sizes in the water of three culture modes of Litopenaeus vannamei, including high-level pond culture, biofloc technology (BFT), and pond culture. The 16S rRNA gene high-throughput sequencing results indicated that the taxa particularly enriched by high-level pond culture modes were mainly Deltaproteobacteria, while Alpha- and Gammaproteobacteria and Flavobacteria were enriched in the BFT culture modes. The pond culture enriched Bacteroidetes, Sphingobacteriia, Actinobacteria, and Cyanobacteria. PCoA analysis showed that for the pond samples, there were significant differences in the community composition compared with the samples from the other two modes. However, the high-level pond and biofloc samples showed similar community compositions. Furthermore, Canonical Correspondence Analysis (CCA) and Variance Partitioning Analysis (VPA) revealed that NH₄⁺-N, salinity (Sal), and NO₃⁻-N were key factors affecting the aquaculture communities. Full article

In the present study, literature information on the functioning of the biofloc technology (BFT) system, its components, the state of the organism of hydrobionts, and water quality is analyzed. It is shown that this technology allows reducing financial costs for water treatment by 30%, increasing the efficiency of protein assimilation in the feed composition by two times, and creating a high-protein substrate, which can be further used as a component of feed for aquaculture. The BFT contains a large number of microorganisms, including photoautotrophic microorganisms (algae), chemoautotrophic microorganisms (nitrifying bacteria), and heterotrophic microorganisms (fungi, infusoria, protozoa, and zooplankton). This technology contributes to the improvement in water quality, aquaculture productivity, and hydrobionts. Despite the higher initial costs, BFT can yield higher economic profits. In this paper, the authors summarize data from many recent studies devoted to BFT. Based on the analysis of a number of studies, it can be concluded that this technology has a high potential for scaling up in industrial aquaculture. Full article

This review aims to study the applications of sensors for monitoring and controlling the physicochemical parameters of water in aquaculture systems such as Biofloc Technology (BFT), Recirculating Aquaculture Systems (RASs), and aquaponic systems using IoT technology, as well as identify potential knowledge gaps. A bibliometric analysis and systematic review were conducted using the Scopus database between 2020 and 2024. A total of 217 articles were reviewed and analyzed. Our findings indicated a significant increase (74.79%) in research between 2020 and 2024. pH was the most studied physicochemical parameter in aquaculture, analyzed in 98.2% of cases (sensors: SEN0169, HI-98107, pH-4502C, Grove-pH), followed by temperature (92.9%, sensor DS18B20) and dissolved oxygen (62.5%, sensors: SEN0237, MAX30102, OxyGuard DO model 420, ZTWL-SZO2-485, Lutron DO-5509). Overall, water monitoring through the implementation of IoT sensors improved growth rates, reduced culture mortality rates, and enabled the rapid prediction and detection of atypical Total Ammonia Nitrogen (TAN) levels. IoT sensors for water quality monitoring in aquaponics also facilitate the evaluation and prediction of seed and vegetable growth and germination. In conclusion, despite recent advancements, challenges remain in automating parameter control, ensuring effective sensor maintenance, and improving operability in rural areas, which need to be addressed. Full article

The aquaculture sector is experiencing remarkable growth, and its economic success depends mainly on an efficient production strategy and cost management, which are essential to guarantee the profitability and sustainability in this sector. The main objective of this study was to analyze the production costs and growth performance of Oreochromis niloticus in the most successful intensive production systems: Recirculating Aquaculture System (RAS), Aquaponic System (AS), and Biofloc Technology (BFT). Data collection involved extensive searches in various academic and scientific databases, resulting in the selection of 52 published articles from 2008 to 2024, following a rigorous analysis of inclusion criteria. Results showed that the rentability of the aquaculture production systems for Tilapia production is underexplored in the scientific community, with less than 20% of articles reporting economic variables, while around 80% of them reported growing variables and water quality without considering economic variables. Costs associated with production and economic indicators must also be viewed as important indicators for aquaculture producers as a reference for the investment in RAS, AS, and BFT technologies. The research in the aquaculture field has seen a resurgence in studies on production, design, water quality, and the recent integration of technologies to increase production. Full article

Biofloc technology (BFT) is an advanced aquaculture method that uses microbial communities to enhance water quality and support aquatic species cultivation. Our research aims to delve into the pivotal role of aeration intensity within BFT systems, revealing its influence on microbial community structures, water quality, and nutrient cycling for L. vannamei culture. Three aeration levels were set with intensities of V75 (75 L/min), V35 (35 L/min), and V10 (10 L/min). The results showed that the lowest aeration intensity (V10) resulted in larger floc sizes and a reduction in the 2D-fractal dimensions, indicating a decreased overall structural complexity of the bioflocs. In addition, water quality parameters, including total ammonia nitrogen and nitrite, remained low across all treatments, highlighting the water-purifying capacity of biofloc. While protein and lipid contents in biofloc did not differ significantly among treatments, docosahexaenoic acid (DHA) levels were highest in the V75 treatment, suggesting that higher aeration promotes the accumulation of essential fatty acids. RDA analysis revealed that microorganisms like Ruegeria sp. and Sulfitobacter mediterraneus negatively correlated with ammonia and nitrite levels, suggesting their key role in converting ammonia to nitrite and nitrate in marine nitrogen cycles. The functional annotation of metagenomes across different aeration levels showed the similarly active roles of microorganisms in nitrogen metabolism and protein synthesis. In conclusion, while variations in aeration intensity affect floc size and the accumulation of essential fatty acids in biofloc, they do not significantly impact overall water quality or core microbial functions in L. vannamei aquaculture. Future research should focus on the effects of aeration strategies on microbial community dynamics and the integration of these data with performance metrics in L. vannamei. These insights can help optimize biofloc cultivation and enhance environmental sustainability in the aquaculture industry. Full article

This study evaluated water quality, growth, and partial budget analysis (PBA) for Penaeus vannamei, comparing super-intensive Biofloc Technology (BFT) and Recirculating Aquaculture Systems (RAS). The 69-day trial used 100 L units with two treatments (RAS and BFT), each with three replicates. Shrimp were initially reared in a 30-day nursery to a weight of 0.10 ± 0.04 g and then stocked at 500 shrimp m⁻³. Biofloc growth in BFT was promoted by maintaining a C:N ratio of 15:1, adding dextrose when total ammonia nitrogen (TAN) reached 1 mg L⁻¹. Probiotics (3 g m⁻³) were administered daily to both groups. TAN levels in BFT initially spiked but stabilized after 36 days. Vibrio abundance was initially higher in RAS, but by the end of the trial, it was higher in BFT. Final weight, weekly growth ratio, and yield were greater in BFT, whereas feed conversion ratio (FCR) and water use were higher in RAS. Survival rates were 83.33% in BFT and 88% in RAS. BFT achieved a superior net benefit/cost compared to RAS. Although RAS more effectively controlled nitrogenous compounds, BFT exhibited better growth performance, with higher final weights, lower FCR, and better Vibrio management. The partial budget analysis indicated an economic advantage for BFT, with a net positive benefit of $2270.09 when shifting from RAS to BFT due to lower operating costs and higher shrimp yield. Among these two sustainable production systems, BFT was more productive while utilizing less natural resources. Full article