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1,550 Results Found

  • Article
  • Open Access
1,740 Views
6 Pages

In this paper, a new technique to recognize and process Barcodes is introduced. The technique employs Inductive Learning. It is suitable to use, for example, in a factory to control the workers, staff, stock etc. In this technique only vertical lines...

  • Article
  • Open Access
27 Citations
4,105 Views
16 Pages

OTU Delimitation with Earthworm DNA Barcodes: A Comparison of Methods

  • Arnaud Goulpeau,
  • Benoit Penel,
  • Marie-Eugénie Maggia,
  • Daniel Fernández Marchán,
  • Dirk Steinke,
  • Mickaël Hedde and
  • Thibaud Decaëns

13 October 2022

Although DNA barcodes-based operational taxonomic units (OTUs) are increasingly used in earthworm research, the relative efficiency of the different methods available to delimit them has not yet been tested on a comprehensive dataset. For this study,...

  • Article
  • Open Access
2,169 Views
14 Pages

Hardware Acceleration of Identifying Barcodes in Multiplexed Nanopore Sequencing

  • Wenjie Hu,
  • Yuxin Zhang,
  • Hongrui Zhang and
  • Weigang Chen

19 August 2022

In multiplexed sequencing, the identification of DNA sequencing barcodes can effectively reduce the probability of sample misassignment. However, the great quantity of sequence data requires a high-throughput identification method. Therefore, based o...

  • Article
  • Open Access
27 Citations
8,116 Views
16 Pages

DNA Barcodes for Accurate Identification of Selected Medicinal Plants (Caryophyllales): Toward Barcoding Flowering Plants of the United Arab Emirates

  • Rahul Jamdade,
  • Kareem A. Mosa,
  • Ali El-Keblawy,
  • Khawla Al Shaer,
  • Eman Al Harthi,
  • Mariam Al Sallani,
  • Mariam Al Jasmi,
  • Sanjay Gairola,
  • Hatem Shabana and
  • Tamer Mahmoud

30 March 2022

The need for herbal medicinal plants is steadily increasing. Hence, the accurate identification of plant material has become vital for safe usage, avoiding adulteration, and medicinal plant trading. DNA barcoding has shown to be a valuable molecular...

  • Review
  • Open Access
160 Citations
19,495 Views
23 Pages

DNA Barcodes for Marine Biodiversity: Moving Fast Forward?

  • Adriana E. Radulovici,
  • Philippe Archambault and
  • France Dufresne

25 March 2010

‘Biodiversity’ means the variety of life and it can be studied at different levels (genetic, species, ecosystem) and scales (spatial and temporal). Last decades showed that marine biodiversity has been severely underestimated at all levels. In order...

  • Article
  • Open Access
722 Views
15 Pages

Untangling Coelogyne: Efficacy of DNA Barcodes for Species and Genus Identification

  • Małgorzata Karbarz,
  • Faustyna Grzyb,
  • Dominika Szlachcikowska and
  • Agnieszka Leśko

10 November 2025

Background/Objectives: While morphological similarity and incomplete specimens pose a challenge to the precise identification of Coelogyne orchids, accurate species and genus assignment is essential for conservation and CITES enforcement. This study...

  • Communication
  • Open Access
1 Citations
1,819 Views
14 Pages

13 December 2024

Data storage on DNA has emerged as a molecular approach to safeguarding digital information. Microarrays are an excellent source of complex DNA sequence libraries and are playing a central role in the development of this technology. However, the amou...

  • Article
  • Open Access
7 Citations
3,834 Views
23 Pages

4 October 2022

One of the most popular tools for species discovery and resolution is the DNA barcode, typically based on the cytochrome c oxidase I (COI) gene. However, other non-genic barcodes are available for Diptera. The banding sequence of polytene chromosomes...

  • Article
  • Open Access
16 Citations
6,833 Views
14 Pages

Assessment of ITS1, ITS2, 5′-ETS, and trnL-F DNA Barcodes for Metabarcoding of Poaceae Pollen

  • Denis O. Omelchenko,
  • Anastasia A. Krinitsina,
  • Artem S. Kasianov,
  • Anna S. Speranskaya,
  • Olga V. Chesnokova,
  • Svetlana V. Polevova and
  • Elena E. Severova

5 March 2022

Grass pollen is one of the major causes of allergy. Aerobiological monitoring is a necessary element of the complex of anti-allergic measures, but the similar pollen morphology of Poaceae species makes it challenging to discriminate species in airbor...

  • Article
  • Open Access
31 Citations
6,484 Views
12 Pages

5 March 2022

The subfamily Entiminae is the largest group in the family Curculionidae, and it has long represented a challenge in traditional and molecular classification. Here, we analyzed intra- and interspecific genetic distances of 621 public COI barcode sequ...

  • Article
  • Open Access
2 Citations
3,913 Views
19 Pages

Survey of Lichenized Fungi DNA Barcodes on King George Island (Antarctica): An Aid to Species Discovery

  • Renato Daniel La Torre,
  • Daniel Ramos,
  • Mayra Doris Mejía,
  • Edgar Neyra,
  • Edwin Loarte and
  • Gisella Orjeda

11 May 2023

DNA barcoding is a powerful method for the identification of lichenized fungi groups for which the diversity is already well-represented in nucleotide databases, and an accurate, robust taxonomy has been established. However, the effectiveness of DNA...

  • Article
  • Open Access
14 Citations
4,400 Views
27 Pages

11 December 2020

Methods of topological data analysis have been successfully applied in a wide range of fields to provide useful summaries of the structure of complex data sets in terms of topological descriptors, such as persistence diagrams. While there are many po...

  • Article
  • Open Access
6 Citations
5,314 Views
20 Pages

EasyStego: Robust Steganography Based on Quick-Response Barcodes for Crossing Domains

  • Zhenhao Luo,
  • Wei Xie,
  • Baosheng Wang,
  • Yong Tang and
  • Qianqian Xing

14 February 2019

Despite greater attention being paid to sensitive-information leakage in the cyberdomain, the sensitive-information problem of the physical domain remains neglected. Anonymous users can easily access the sensitive information of other users, such as...

  • Article
  • Open Access
15 Citations
3,179 Views
12 Pages

Licorice Germplasm Resources Identification Using DNA Barcodes Inner-Variants

  • Qianwen Liu,
  • Shuai Guo,
  • Xiasheng Zheng,
  • Xiaofeng Shen,
  • Tianyi Zhang,
  • Baosheng Liao,
  • Wenrui He,
  • Haoyu Hu,
  • Ruiyang Cheng and
  • Jiang Xu

28 September 2021

Based on the gradual transformation from wild growth to artificial cultivation, the accurate authentication of licorice seeds contributes to the first committed step of its quality control and is pivotal to ensure the clinical efficacy of licorice. H...

  • Article
  • Open Access
3 Citations
2,093 Views
22 Pages

Genotypic Identification of Trees Using DNA Barcodes and Microbiome Analysis of Rhizosphere Microbial Communities

  • Liliana Hopkins,
  • Kayla Yim,
  • Ana Rumora,
  • Melissa F. Baykus,
  • Luisa Martinez and
  • Luis Jimenez

1 July 2024

DNA barcodes can provide accurate identification of plants. We used previously reported DNA primers targeting the internal transcribed spacer (ITS1) region of the nuclear ribosomal cistron, internal transcribed spacer (ITS2), and chloroplast trnL (UA...

  • Article
  • Open Access
11 Citations
3,389 Views
17 Pages

DNA-Based Identification of Eurasian Vicia Species Using Chloroplast and Nuclear DNA Barcodes

  • Irene Bosmali,
  • Georgios Lagiotis,
  • Nadia Haider,
  • Maslin Osathanunkul,
  • Costas Biliaderis and
  • Panagiotis Madesis

31 March 2022

Many legume species of the Vicia L. genus (Fabaceae Lindl.) are key components of the Mediterranean diet and have an integral role in sustainable agriculture. Given the importance of the Vicia species for Eurasian culture, it is necessary to implemen...

  • Article
  • Open Access
4 Citations
3,960 Views
5 Pages

Data Release: DNA Barcodes of Plant Species Collected for the Global Genome Initiative for Gardens (GGI-Gardens) II

  • Morgan R. Gostel,
  • Mónica M. Carlsen,
  • Amanda Devine,
  • Katharine B. Barker,
  • Jonathan A. Coddington and
  • Julia Steier

23 March 2022

The Global Genome Initiative for Gardens (GGI-Gardens) is an international partnership of botanic gardens and arboreta that aims to preserve and understand the genomic diversity of plants on Earth. GGI-Gardens has organized a collection program focus...

  • Article
  • Open Access
653 Views
8 Pages

First Annotated DNA Barcodes for Four Saproxylophagous Beetle Species (Coleoptera: Buprestidae, Cerambycidae) from the Central Valleys of Oaxaca, Mexico

  • Jose Guadalupe Martinez-Hernandez,
  • Matthias Rös,
  • Sonia Trujillo-Argueta,
  • Roberto Reyes-Gonzalez,
  • Paola A. Gonzalez-Vanegas and
  • RF del Castillo

19 December 2025

DNA barcode sequences remain unavailable for many species of Mexican Coleoptera. This study presents the first COI barcode records for four saproxylophagous beetle species (Coleoptera: Buprestidae, Cerambycidae) from the Central Valleys of Oaxaca, Me...

  • Article
  • Open Access
4 Citations
1,969 Views
13 Pages

27 June 2024

The genus Chamaecyparis comprises seven species (C. formosensis, C. hodginsii, C. lawsoniana, C. nootkatensis (Callitropsis nootkatensis), C. obtusa, C. pisifera, and C. thyoides). Accurate species identification is necessary for proper use and econo...

  • Article
  • Open Access
5 Citations
8,654 Views
14 Pages

DNA Barcodes of Asian Houbara Bustard (Chlamydotis undulata macqueenii)

  • Ibrahim A. Arif,
  • Haseeb A. Khan,
  • Joseph B. Williams,
  • Mohammad Shobrak and
  • Waad I. Arif

22 February 2012

Populations of Houbara Bustards have dramatically declined in recent years. Captive breeding and reintroduction programs have had limited success in reviving population numbers and thus new technological solutions involving molecular methods are esse...

  • Article
  • Open Access
9 Citations
3,759 Views
17 Pages

15 September 2023

The need for multi-gene analysis in evolutionary and taxonomic studies is generally accepted. However, the sequencing of multiple genes is not always possible. For various reasons, short mitochondrial DNA barcodes are the only source of molecular inf...

  • Article
  • Open Access
20 Citations
5,336 Views
16 Pages

26 January 2023

The introduction of non-indigenous species (NIS) is one of the major threats to the integrity of European coastal ecosystems. DNA-based assessments have been increasingly adopted for monitoring NIS. However, the accuracy of DNA-based taxonomic assign...

  • Article
  • Open Access
14 Citations
4,324 Views
20 Pages

Evaluation of Arabian Vascular Plant Barcodes (rbcL and matK): Precision of Unsupervised and Supervised Learning Methods towards Accurate Identification

  • Rahul Jamdade,
  • Maulik Upadhyay,
  • Khawla Al Shaer,
  • Eman Al Harthi,
  • Mariam Al Sallani,
  • Mariam Al Jasmi and
  • Asma Al Ketbi

13 December 2021

Arabia is the largest peninsula in the world, with >3000 species of vascular plants. Not much effort has been made to generate a multi-locus marker barcode library to identify and discriminate the recorded plant species. This study aimed to determ...

  • Article
  • Open Access
1 Citations
2,164 Views
15 Pages

4 December 2024

DNA barcoding is an effective modern tool in taxonomy, evolutionary biology, and biodiversity research. Many new species have been discovered and described with DNA barcodes as part of their diagnostic features. We combined morphological examination...

  • Article
  • Open Access
5 Citations
10,166 Views
12 Pages

In this study, a 6-axis robotic arm, which was controlled by an embedded Raspberry Pi with onboard WiFi, was developed and fabricated. A mobile application (APP), designed for the purpose, was used to operate and monitor a robotic arm by means of a W...

  • Article
  • Open Access
4 Citations
3,579 Views
14 Pages

Screening of the Candidate DNA Barcodes for Three Important Amorphophallus Species Identification

  • Chufeng Zhao,
  • Xuan She,
  • Erxi Liu,
  • Nunung Harijati,
  • Teng Cheng,
  • Zhongli Hu,
  • Surong Jin and
  • Ying Diao

11 September 2020

Amorphophallus is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. Amorphophallus has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challen...

  • Article
  • Open Access
2 Citations
3,113 Views
13 Pages

The suspension array technology (SAT) is promising for high-sensitivity multiplexed analysis of tumor markers. Barcodes as the core elements of SAT, can generate encoding fluorescence signals (EFS) and detection fluorescence signals (DFS) in the corr...

  • Feature Paper
  • Article
  • Open Access
5 Citations
4,326 Views
22 Pages

A Non-Destructive High-Speed Procedure to Obtain DNA Barcodes from Soft-Bodied Insect Samples with a Focus on the Dipteran Section of Schizophora

  • Frederik Stein,
  • Stefan Wagner,
  • Nadine Bräsicke,
  • Oliver Gailing,
  • Carina C. M. Moura and
  • Monika Götz

27 July 2022

While the need for biodiversity research is growing, paradoxically, global taxonomical expertise is decreasing as a result of the neglected funding for young academics in taxonomy. Non-destructive approaches for DNA barcoding are necessary for a more...

  • Article
  • Open Access
3 Citations
2,407 Views
24 Pages

Comparative Mitogenomics Provides Valuable Insights for the Phylogeny and New DNA Barcodes of Ganoderma

  • Ti-Qiang Chen,
  • Chi Yang,
  • Xiao-Lan Xu,
  • Lin Yang,
  • Huan-Qing He,
  • Meng-Ting Weng,
  • Zheng-He Ying,
  • Xiao-Kun Shi and
  • Meng-Guang Ding

5 November 2024

Ganoderma is the most important genus in the family Ganodermataceae; many species have attracted much attention and widely cultivated because of their medicinal values, but so far, not a sequenced mitogenome derived from dikaryon strains has been exp...

  • Feature Paper
  • Article
  • Open Access
8 Citations
3,295 Views
27 Pages

24 February 2023

The genus Microtendipes Kieffer (Diptera: Chironomidae) has a nearly worldwide distribution, comprising more than 60 species, which are further divided into two species groups based on larval stage. However, species delimitation and identification am...

  • Article
  • Open Access
1 Citations
2,394 Views
20 Pages

Insights from DNA Barcodes-Based Phylogenetic Analysis of Medicinal Plants and Estimation of Their Conservation Status: A Case Study in the Tianshan Wild Forest, China

  • Aerguli Jiamahate,
  • Tohir A. Bozorov,
  • Jiancheng Wang,
  • Jianwei Zhang,
  • Hongxiang Zhang,
  • Xiyong Wang,
  • Honglan Yang and
  • Daoyuan Zhang

1 January 2025

The Tianshan wild fruit forest region is a vital repository of plant biodiversity, particularly rich in the unique genetic resources of endemic medicinal plants in this ecological niche. However, human activities such as unregulated mining and excess...

  • Article
  • Open Access
453 Views
32 Pages

8 December 2025

Despite extensive research into green microalgae belonging to the genus Chloroidium, their species diversity and biotechnological potential remain poorly characterized. The strain VKM Al-418, the subject of this study, was isolated from the soil of D...

  • Article
  • Open Access
117 Views
15 Pages

High-Resolution Integrative Delimitation of Intertidal Limpets via Multi-Locus Barcodes and SEM Morphology

  • Jialong Liang,
  • Kexin Zhao,
  • Xiaonan Ma,
  • Jiayi Zang,
  • Wenxiao Guo and
  • Ran Zhao

19 January 2026

Limpets are marine gastropod molluscs well adapted to intertidal rocky environments, yet their taxonomic resolution remains challenging due to extensive morphological convergence and the presence of cryptic species. In this study, we applied an integ...

  • Article
  • Open Access
17 Citations
5,104 Views
18 Pages

The need for a comparative analysis of natural metagenomes stimulated the development of new methods for their taxonomic profiling. Alignment-free approaches based on the search for marker k-mers turned out to be capable of identifying not only speci...

  • Article
  • Open Access
1,296 Views
15 Pages

Forensic Identification of Cannabis with Plant DNA Barcodes and Cannabinoid Synthesis Genes

  • Ping Xiang,
  • Yu Wei Phua,
  • Afiqah Razanah Rosli,
  • Kar Jun Loh and
  • Christopher Kiu-Choong Syn

2 November 2025

Background/Objectives: According to the World Drug Report 2025, cannabis is the most abused drug in the world, being sold in illicit markets in various physical forms ranging from herbal cannabis to cannabis resin and liquid cannabis. Currently, the...

  • Article
  • Open Access
15 Citations
2,935 Views
17 Pages

29 April 2024

Numerous Cymbidium species have significant commercial value globally due to their exotic ornamental flowers. Identifying Cymbidium species is challenging due to their similar shapes, which hinders their rational use and the conservation of germplasm...

  • Article
  • Open Access
14 Citations
4,715 Views
15 Pages

19 September 2019

Short, standardized gene fragments for species identification (DNA barcodes) have proven effective in delineating closely-related insect species, and can be critical characters to include in taxonomic studies. This is also the case for the species-ri...

  • Article
  • Open Access
3 Citations
4,906 Views
24 Pages

The Mosquitoes of Morelos, Mexico: DNA Barcodes, Distribution, Ecology and the Resurrection of the Name Culiseta dugesi Dyar and Knab (Diptera: Culicidae)

  • Aldo I. Ortega-Morales,
  • Luis M. Hernández-Triana,
  • Javier A. Garza-Hernández,
  • Carlos M. Ramírez-Huicochea,
  • Andrés J. Martínez-Gaona,
  • J. Manuel Quijano-Barraza,
  • Cassandra González-Acosta and
  • Fabián Correa-Morales

26 April 2024

To update the record of mosquito diversity in the state of Morelos, Mexico, mosquito collections were conducted in both physiographical regions of Morelos: the Neo-volcanic Axis and the South Mountain Range. Immature stages were collected from aquati...

  • Article
  • Open Access
1 Citations
2,228 Views
34 Pages

Tachinid Flies (Diptera), Caterpillar Hosts (Lepidoptera) and Their Food Plants, Reared in Área de Conservación Guanacaste (ACG), Northwestern Costa Rica: Documenting Community Structure with the Aid of DNA Barcodes

  • Donald L. J. Quicke,
  • Alan J. Fleming,
  • D. Monty Wood,
  • Norman E. Woodley,
  • Ramya Manjunath,
  • Suresh Naik,
  • M. Alex Smith,
  • Michael J. Sharkey,
  • Winnie Hallwachs and
  • Buntika A. Butcher
  • + 4 authors

20 September 2025

We describe the trophic relationships of tachinid parasitoid flies that attack exophagous, leaf-eating Lepidoptera caterpillars in Área de Conservación Guanacaste (ACG), northwestern Costa Rica over approximately forty years beginning i...

  • Article
  • Open Access
22 Citations
6,604 Views
17 Pages

Check Your Shopping Cart: DNA Barcoding and Mini-Barcoding for Food Authentication

  • Tommaso Gorini,
  • Valerio Mezzasalma,
  • Marta Deligia,
  • Fabrizio De Mattia,
  • Luca Campone,
  • Massimo Labra and
  • Jessica Frigerio

16 June 2023

The molecular approach of DNA barcoding for the characterization and traceability of food products has come into common use in many European countries. However, it is important to address and solve technical and scientific issues such as the efficien...

  • Article
  • Open Access
15 Citations
4,211 Views
14 Pages

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

  • Linyu Zheng,
  • Yue Zhang,
  • Wenzhao Yang,
  • Yiying Zeng,
  • Fan Jiang,
  • Yujia Qin,
  • Jiafeng Zhang,
  • Zhaochun Jiang,
  • Wenzhao Hu and
  • Zhihong Li
  • + 4 authors

12 December 2019

Tephritidae fruit flies (Diptera: Tephritidae) are regarded as important damage-causing species due to their ability to cause great economic losses in fruit and vegetable crops. Bactrocera minax and Bactrocera tsuneonis are two sibling species of the...

  • Review
  • Open Access
71 Citations
31,164 Views
12 Pages

DNA Barcoding as a Plant Identification Method

  • Stavroula Letsiou,
  • Panagiotis Madesis,
  • Efstathios Vasdekis,
  • Cinzia Montemurro,
  • Maria E. Grigoriou,
  • George Skavdis,
  • Vassilios Moussis,
  • Antonios E. Koutelidakis and
  • Andreas G. Tzakos

8 February 2024

In the last two decades, plant taxonomy has bloomed, following the development of a novel technique, namely, DNA barcoding. DNA barcodes are standardized sequences, ideally unique, coding or non-coding, either from the genome of the organism or from...

  • Article
  • Open Access
7 Citations
2,711 Views
12 Pages

8 July 2023

With the birth of next-generation sequencing (NGS) technology, genomic data in public databases have increased exponentially. Unfortunately, exogenous contamination or intracellular parasite sequences in assemblies could confuse genomic analysis. Mea...

  • Article
  • Open Access
5 Citations
3,090 Views
13 Pages

27 January 2023

Ficus simplicissima Lour. is an Asian species of fig tree in the family Moraceae. The chloroplast (cp) genome of F. simplicissima m3 was sequenced using the Pacbio sequel platform. The F. simplicissima cpDNA has a size of 160,321 bp in length, of whi...

  • Article
  • Open Access
23 Citations
12,305 Views
12 Pages

9 August 2019

Vision-based 1D barcode reading has been the subject of extensive research in recent years due to the high demand for automation in various industrial settings. With the aim of detecting the image region of 1D barcodes, existing approaches are both s...

  • Article
  • Open Access
1,527 Views
59 Pages

17 November 2025

The aim of this study was to supplement current knowledge on the species diversity of Opostegidae in Central America and the Caribbean and to compare this diversity with that of other regions. We examined historical material and conducted fieldwork i...

  • Article
  • Open Access
10 Citations
4,461 Views
11 Pages

Molecular Species Delimitation Using COI Barcodes of Mealybugs (Hemiptera: Pseudococcidae) from Coffee Plants in Espírito Santo, Brazil

  • Pablo Viana Oliveira,
  • Alexandre Rosa dos Santos,
  • Emily Lopes Olive,
  • Karolinni Bianchi Britto,
  • Francine Alves Nogueira de Almeida,
  • Vitor Cezar Pacheco da Silva,
  • Carolina Barros Machado,
  • Maurício José Fornazier,
  • José Aires Ventura and
  • Greiciane Gaburro Paneto
  • + 1 author

20 February 2023

Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-sp...

  • Review
  • Open Access
84 Citations
18,258 Views
16 Pages

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

  • Gianni Barcaccia,
  • Margherita Lucchin and
  • Martino Cassandro

29 December 2015

DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Al...

  • Article
  • Open Access
36 Citations
8,242 Views
22 Pages

DNA Barcoding Medicinal Plant Species from Indonesia

  • Ria Cahyaningsih,
  • Lindsey Jane Compton,
  • Sri Rahayu,
  • Joana Magos Brehm and
  • Nigel Maxted

21 May 2022

Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification pur...

  • Article
  • Open Access
11 Citations
7,593 Views
27 Pages

14 November 2022

Recent advancement in Deep Learning-based Convolutional Neural Networks (D-CNNs) has led research to improve the efficiency and performance of barcode recognition in Supply Chain Management (SCM). D-CNNs required real-world images embedded with groun...

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