Search Results (165)

Radopholus similus, commonly known as the burrowing nematode, is one of the major pathogens affecting banana production. Currently, the control of this pathogen relies on chemicals, as no resistant varieties are available. However, new control methods, such the application of ensilage biostimulants (EBs) near the banana rhizosphere, have shown effectiveness. Nevertheless, the impact of this organic control method on soil nematodes and other microbial components remains unknown. This study evaluates the effects of EB application on the native nematofauna of banana. EBs altered the flow of carbon, nutrients, and energy in ways that influenced the abundance of fungivorous and bacterivorous taxa, while consistently reducing the number of plant-parasitic nematodes throughout the experimental period. Specifically, EB application in the soil increased the abundance of certain free-living nematodes, including Aphelenchus, Aphelenchoides, Cephalobidae, and Rhabditidae, while decreasing both the abundance and diversity of phytoparasitic nematodes. In contrast, Criconematidae, Hoplolaimidae, Meloidogyne, Tylenchidae, and R. similis were more abundant in the control and oxamyl-treated soils. EBs can play a crucial role in strategies aimed to improve soil resilience, fertility, and natural suppression, provided that more sustainable production practices are adopted. Full article

Background and Objectives: In recent years, there has been growing interest in the production of ingredients rich in dietary fiber and antioxidants, such as green banana flours. This study evaluated the effect of consumption of mixed green banana pulp (PF) and peel (PeF) flours on the body weight gain, adiposity, lipid profile, and intestinal morphology of Wistar rats. Methods: Male young rats were divided into four groups (n = 8) that received a standard diet (SD), or one of the following three test diets: M1 (SD + 90% PF/10% PeF), M2 (SD + 80% PF/20% PeF), or P (SD + 100% PF) for 28 days. Results: Rats from M1, M2, and P groups showed reduced body weight gain and adiposity and had lower contents of total cholesterol, LDL-c, VLDL-c, and triglycerides. Animals from M1 and M2 groups had an increase in cecum weight, fecal moisture, acetic acid concentration, and crypt depth and reduced fecal pH. Moreover, consumption of the M1, M2, and P diets increased the expression of proteins involved in intestinal functionality. Significant negative correlations were observed between consumption of resistant starch and soluble dietary fiber, from the flours, and weight gain (r = −0.538 and r = −0.538, respectively), body adiposity (r = −0.780 and r = −0.767, respectively), total cholesterol (r = −0.789 and r = −0.800, respectively), and triglycerides (r = −0.790 and r = −0.786, respectively). Conclusions: Mixed green banana pulp and peel flour proved to be a viable alternative as a food ingredient that can promote weight loss, improve lipid profile and intestinal morphology, and minimize post-harvest losses. Full article

Unconventional food plants (UFPs) are increasingly valued for their nutritional composition and bioactive potential. This study proposes a comprehensive characterization of the chemical and bioactive properties of Pereskia aculeata Miller (Cactaceae) (PA); Xanthosoma sagittifolium (L.) Schott (Araceae) (XS); Stachys byzantina K. Koch (Lamiaceae) (SB); and inflorescences from three cultivars of Musa acuminata (Musaceae) var. Dwarf Cavendish, var. BRS Platina, and var. BRS Conquista (MAD, MAP, and MAC), including the assessment of physical, nutritional, phytochemical, and biological parameters. Notably, detailed phenolic profiles were established for these species, many of which are poorly documented in the literature. XS was characterized by a unique abundance of C-glycosylated flavones, especially apigenin and luteolin derivatives, rarely described for this species. SB exhibited high levels of phenylethanoid glycosides, particularly verbascoside and its isomers (up to 21.32 mg/g extract), while PA was rich in O-glycosylated flavonols such as quercetin, kaempferol, and isorhamnetin derivatives. Nutritionally, XS had the highest protein content (16.3 g/100 g dw), while SB showed remarkable dietary fiber content (59.8 g/100 g). Banana inflorescences presented high fiber (up to 66.5 g/100 g) and lipid levels (up to 7.35 g/100 g). Regarding bioactivity, PA showed the highest DPPH radical scavenging activity (95.21%) and SB the highest reducing power in the FRAP assay (4085.90 µM TE/g). Cellular antioxidant activity exceeded 2000% in most samples, except for SB. Cytotoxic and anti-inflammatory activities were generally low, with only SB showing moderate effects against Caco-2 and AGS cell lines. SB and PA demonstrated the strongest antimicrobial activity, particularly against Yersinia enterocolitica, methicillin-resistant Staphylococcus aureus (MRSA), and Enterococcus faecalis, with minimum inhibitory concentrations ranging from 0.156 to 0.625 mg/mL. Linear discriminant analysis revealed distinctive chemical patterns among the species, with organic acids (e.g., oxalic up to 7.53 g/100 g) and fatty acids (e.g., linolenic acid up to 52.38%) as key discriminant variables. Overall, the study underscores the nutritional and functional relevance of these underutilized plants and contributes rare quantitative data to the scientific literature regarding their phenolic signatures. Full article

The sterol demethylation inhibitors (DMIs) are among the most widely used fungicides for controlling black Sigatoka (Mycosphaerella fijiensis) and yellow Sigatoka (Mycosphaerella musicola) in banana plantations in Brazil. Black Sigatoka is considered more important due to causing yield losses of up to 100% in commercial banana crops under predisposing conditions. In contrast, yellow Sigatoka is important due to its widespread occurrence in the country. This study aimed to determine the current sensitivity levels of Mf and Mm populations to DMI fungicides belonging to the chemical group of triazoles. Populations of both species were sampled from commercial banana plantations in Registro, Vale do Ribeira, São Paulo (SP), Ilha Solteira, Northwestern SP, and Janaúba, Northern Minas Gerais, and were further characterized phenotypically. Additionally, allelic variation in the CYP51 gene was analyzed in populations of these pathogens to identify and characterize major mutations and/or mechanisms potentially associated with resistance. Sensitivity to the triazoles propiconazole and tebuconazole was determined by calculating the 50% inhibitory concentration of mycelial growth (EC₅₀) based on dose–response curves ranging from 0 to 5 µg mL⁻¹. Variation in sensitivity to fungicides was evident with all nine Mf isolates showing moderate resistance levels to both propiconazole or tebuconazole, while 11 out of 42 Mm strains tested showed low to moderate levels of resistance to these triazoles. Mutations leading to CYP51 substitutions Y136F, Y461N/H, and Y463D in Mm and Y461D, G462D, and Y463D in Mf were associated with low or moderate levels of resistance to the triazoles. Interestingly, Y461H have not been reported before in Mm or Mf populations, and this alteration was found in combination with V106D and A446S. More complex CYP51 variants and CYP51 promoter inserts associated with upregulation of the target protein were not detected and can explain the absence of highly DMI-resistant strains in Brazil. Disease management programs that minimize reliance on fungicide sprays containing triazoles will be needed to slow down the further evolution and spread of novel CYP51 variants in Mf and Mm populations in Brazil. Full article

Organic farming can help mitigate the negative impacts of agriculture on biodiversity, but its effects remain controversial and poorly understood for many taxa, especially from a genetic perspective, where major knowledge gaps persist. This study investigates how the organic and conventional management of banana groves influences population dynamics (i.e., total abundance and abundance by sex and developmental stage) and mitochondrial genetic diversity (cytochrome b gene) of the earwig Euborellia annulipes, a natural biological control agent. The results revealed higher overall abundance, particularly of females, in organic groves. This could be due to females’ more sedentary behaviour linked to parental care, increasing their vulnerability to local disturbances such as pesticide application. In contrast, males, being more mobile, did not respond to the farming system. Genetic analyses revealed similar haplotypic diversity across systems but higher nucleotide diversity in conventional orchards. This may suggest either pesticide-induced mutations associated with detoxification and resistance or increased gene flow driven by greater mobility in response to disturbance. Although the high genetic diversity observed raises questions about the introduced status of E. annulipes, its origin remains unresolved. These findings highlight the relevance of integrating ecological and genetic data when assessing the impacts of agricultural practices on beneficial arthropods. Full article

Banana wilt disease is an important disease in banana production, causing significant losses. Herein, we investigated the mechanism by which Bacillus velezensis isolate X5 enhances the resistance of different resistant banana cultivars to Fusarium oxysporum f. sp. cubense race 4 (Foc4). From the perspectives of metabolism, transcriptome, and key genes in important pathways, this study analyzed the composition and content changes of other types of signaling molecules, such as free amino acids and soluble sugars, in resistant/susceptible varieties. The results indicate that under pathogen stress, the contents of root-secreted metabolite components in both resistant and susceptible varieties increase significantly overall, and the increase in susceptible varieties is generally higher. For example, the free amino acid components in susceptible varieties are significantly more than those in resistant varieties. However, the addition of biocontrol bacteria can inhibit this increase. Exogenous addition experiments prove that differential metabolites can either promote or inhibit Foc4 and X5 at certain concentrations. The results of KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment and GO (Gene Ontology) annotation show that resistant varieties have more defense pathways compared to susceptible varieties. Under X5 treatment, more defense genes in resistant varieties are activated or their expression is enhanced, promoting the plant roots to secrete more substances related to plant resistance, such as phenylpropanoids and lignin. This research revealed the effects of Bacillus velezensis on bananas and pathogens, allowing valuable conclusions to be drawn. The results have good application potential to understand the relationships among the three species, defining the biocontrol effect and mechanism of Bacillus velezensis, and providing a theoretical basis for the biological control of soil-borne diseases such as banana wilt disease. Full article

This study analyses the effect of lignin extracted from the Dominico-Harton banana on the physicochemical properties of biodegradable methylhydroxyethylcellulose (MHEC) composites. Lignin was obtained by grinding and sieving, followed by treatment with sulphuric acid and subsequent separation via centrifugation. Films were developed using the casting method, incorporating lignin and glycerol in a matrix of MHEC dissolved in distilled water. They were characterised according to their physical, barrier, mechanical, optical, and antioxidant properties, using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The results showed that lignin provides antioxidant capacity and improves mechanical and barrier properties, while MHEC contributes flexibility and biodegradability. These films have a dense and resistant structure, with potential applications in food packaging, agriculture, and medicine. The research highlights the use of agricultural waste to develop sustainable materials as an alternative to conventional plastics. Full article

Plant defensin (PDF/DEF), an important pathogenesis-related protein which widely exists in plants, displays broad-spectrum antifungal activities. To date, however, reports on the banana PDFs are very limited. In this study, we identified, cloned, and characterized the five Class I PDFs (MaPDF2.1~MaPDF2.5) in banana (Musa acuminata). Further, their expression in root, corm, leaf, and fruit were studied. MaPDFs exhibited quite different expression patterns in different organs, with MaPDF2.2 as the only member expressing in all the tested organs, and its expression levels in all organs were the highest among all MaPDFs. The MaPDF2.2 expression could be significantly upregulated by both low- and high-temperature stresses but significantly downregulated by the inoculations of plant growth promoting endophytic fungus Serendipita indica and banana Fusarium wilt (FW) pathogen Fusarium oxysporum f. sp. cubense (Foc) Tropical race 4 (FocTR4). Moreover, the S. indica pre-colonization could significantly alleviate the suppression of FocTR4 on MaPDF2.2, suggesting that this MaPDF might contribute greatly to the S. indica-enhanced FW resistance. By using tobacco leaf transient overexpression, the function of MaPDF2.2 was investigated. Its overexpression significantly inhibited the infection of Foc race 1 (Foc1) and FocTR4 in tobacco leaves. Furthermore, in vitro antifungal ability assays revealed that the recombinant His-MaPDF2.2 protein could significantly inhibit the growth of Foc1 and FocTR4, as well as the pigment accumulation of Foc1. Our study revealed the sequence and expression characteristics of banana PDFs and demonstrated the antifungal ability of MaPDF2.2 to FW pathogens. Full article

Biopolymeric films derived from starch are gaining attention due to their potential applications, which are primarily attributed to their availability and biodegradability. Here, we report developing and characterizing a biopolymeric film utilizing banana peel waste (BM2). Analytical techniques were employed, including water absorption analysis, determination of soluble matter, UV-visible absorption spectrophotometry, tensile strength assessment, morphological examination using scanning electron microscopy (SEM), and thermal analysis through thermogravimetric analysis (TGA). The water absorption analysis revealed a noteworthy absorption percentage of 115.23% and 61.75% of soluble matter. The UV-visible absorption spectrophotometry results demonstrated a light absorbance degree ranging from 0.9 to 720 nm, particularly between 400 and 1000 nm. However, the mechanical strength tests indicated relatively low resistance at 0.8 MPa, attributed to the irregular surface observed in the film’s morphology as evidenced by scanning electron microscopy (SEM). Thermal analysis conducted via TGA offered valuable insights into the degradation behavior of the film. The findings reveal a degradation temperature ranging from 160 to 300 °C, thereby elucidating the thermal stability of the film and its potential applications. While mechanical limitations were evident, the biopolymeric film derived from banana peel waste demonstrated noteworthy water absorption properties, presenting potential in specific applications, particularly those that do not necessitate elevated mechanical strength. Continued efforts in optimizing and refining the film’s structure promise to bolster its mechanical properties, making it suitable for various applications. Full article

This study provides a comprehensive analysis of the physicochemical, structural, and functional properties of starches extracted from five distinct banana varieties. The starches were labeled as follows: ‘Nan Tian Huang’ starch (NS), ‘Da jiao’ starch (DS), ‘Gui jiao’ starch (GS), ‘Gong jiao’ starch (OS), and ‘Hong jiao’ starch (HS). The results show that all starches have A-type crystalline structures and contain high levels of resistant starch, ranging from 88.3% to 93.5%. The amylose content ranges from 21.97% to 55.46%. The starches isolated from the five banana varieties are predominantly flat, rod-shaped, and spherical. Particle sizes vary significantly, ranging from 19.75 to 28.65 µm, which contributes to differences in their functional properties. For example, DS demonstrates exceptional functional properties, including high RS content, a low glycemic index, and excellent thermal stability. In contrast, HS starch, despite its high amylose content, exhibits higher enzymatic digestibility and lower freeze–thaw stability. Principal component analysis and correlation analysis revealed that amylose content, thermal properties, and particle morphology are key determinants of the physicochemical and digestive properties of banana starch, emphasizing their interdependence. Additionally, notable differences were observed in the gelatinization properties, thermal characteristics, crystallization, and textural parameters. These findings offer valuable insights into the potential applications of banana starch in functional foods and industrial products, highlighting the importance of starch type in optimizing its functionality. Full article

Soybean oil is susceptible to thermal deterioration, especially during the deep-frying process due to its high polyunsaturated fatty acids. Soybean oil has been employed to enhance the nutritional profile and thermal stability by simply blending it with other oils, including palm olein, camelia, sesame, and cashew nut oil. In particular, coconut oil is more resistant to oxidation than those oils, so adding it to soybean oil that is prone to oxidation can make the mixture more stable. Therefore, this study aims to investigate the thermal stability of soybean oil by blending it with coconut oil and evaluating the blend’s physicochemical changes during the continuous deep frying of banana chips. Refined soybean oil was blended with refined coconut oil at different ratios (% v/v), including 100:0 (A), 80:20 (B), 70:30 (C), and 60:40 (D). All the mixtures were used for continuous deep frying at a constant temperature of 180 °C. The banana chips were fried for 1 min at 5 min intervals over a total of nine batches. The findings show that changes in the physicochemical properties of the frying oils were significantly affected by the soybean oil to coconut oil ratios and the frying duration, which were analyzed using a two-way analysis of variance (p < 0.05). The alteration in free fatty acids and peroxide values were found to be the lowest in treatment C, followed by D < B < A, by using a two-way analysis of variance (p < 0.05). Conversely, the highest total oxidation value was found in treatment A, followed by B > C > D. The lightness of the oil reached the highest value in the last frying cycle in treatments B and C, followed by D and A, while the color of the fried banana chips achieved the maximum value in treatment D, followed by C < B < A. In addition, the lipid content in the fried banana chips was observed to be the lowest in treatments D and C compared to B and A. This study indicated that blending highly unsaturated soybean oil with coconut oil could enhance its thermal stability. Consequentially, a 70:30 (% v/v) ratio of soybean oil with coconut oil exhibited good thermal stability during continuous deep frying. This study provides insights into an alternative blending technique for soybean and coconut oils to improve the thermal stability of frying oil during continuous deep frying. Full article

In order to obtain a highly nutritious extrudate, a combination of pseudocereals, vegetable protein, and banana flour, a fruit with high sensory acceptability, was used. The objective of the research was to produce a multi-component extrudate (ME) based on cañihua and quinoa with the addition of pea protein isolate and banana flour. The response variables evaluated were composition, expansion, hydration, colour, and hardness properties, as well as the microscopy and sensory characteristics of the flakes produced. These flakes were compared with three commercial extrudates, commercial quinoa-based extrudate (QE), commercial corn-based extrudate (CE), and commercial wheat-based extrudate (WE), which had similar characteristics. The ME showed a higher protein content compared to commercial extrudates (13.60%), and it had significant amounts of lipids, fibre, and ash. The expansion of the ME was like commercial quinoa but significantly lower than the CE and the WE in terms of expansion (p < 0.05). Regarding the absorption and solubility indices of the ME, these indicated that it had lower starch fragmentation compared to the commercial CE and WE. In addition, the instrumental hardness of the ME was higher than the commercial ones due to the complex nature of the product. Through scanning electron microscopy (SEM), it was observed that the ME showed some remaining extrusion-resistant starch granules from quinoa and cañihua with the presence of protein bodies. Finally, the flash profile described the ME as having a pronounced flavour, higher hardness, and lower sweetness, and the free sorting task allowed it to be differentiated from commercial extrudates based on its natural appearance and chocolate flavour. Full article

The banana gene MaWRKY45 gene encodes a WRKY transcription factor (TF) that is closely related to OsWRKY45, which is a master regulator of defense responses in rice. MaWRKY45 is a transcription factor with proven transactivation activity and nuclear localization. Its expression is upregulated by the defense phytohormones salicylic acid (SA) and jasmonic acid (JA). Despite these findings, its transcriptome-wide impact during overexpression remains unexplored. Accordingly, the present study employed the Infiltration-RNAseq method to identify differentially expressed genes (DEGs) resulting from the overexpression of MaWRKY45 in the leaves of the model plant Nicotiana benthamiana. A total of 2473 DEGs were identified in N. benthamiana leaves overexpressing the banana gene MaWRKY45. Of these, 1092 were up-regulated and 1381 were down-regulated. Among the genes that were found to be up-regulated, those encoding proteins that are involved in plant immunity were identified. These included disease resistance receptors, proteins that are involved in cell wall reinforcement, proteins that possess antimicrobial and insecticidal activities, and defense-related TFs. It was thus concluded that the function of the banana gene MaWRKY45 is associated with the plant immune system, and that its overexpression can lead to enhance defense responses. Full article

Banana streak GF virus (BSGFV) is the extremely dangerous monopartite badnavirus (genus, Badnavirus; family, Caulimoviridae) of banana (Musa acuminata AAA Group) that imposes a serious threat to global banana production. The BSGFV causes a devastating pandemic in banana crops, transmitted by deadly insect pest mealybug vectors and replicated through an RNA intermediate. The BSGFV is a reverse-transcribing DNA virus that has a monopartite open circular double-stranded DNA (dsDNA) genome with a length of 7325 bp. RNA interference (RNAi) is a natural mechanism that has revolutionized the target gene regulation of various organisms to combat virus infection. The current study aims to locate the potential target binding sites of banana-encoded microRNAs (mac-miRNAs) on the BSGFV-dsDNA-encoded mRNAs based on three algorithms, RNA22, RNAhybrid and TAPIR. Mature banana (2n = 3x = 33) miRNAs (n = 32) were selected and hybridized to the BSGFV genome (MN296502). Among the 32 targeted mature locus-derived mac-miRNAs investigated, two banana mac-miRNA homologs (mac-miR162a and mac-miR172b) were identified as promising naturally occurring biomolecules to have binding affinity at nucleotide positions 5502 and 9 of the BSGFV genome. The in silico banana-genome-encoded mac-miRNA/mbg-miRNA-regulatory network was developed with the BSGFV—ORFs using Circos software (version 0.69-9) to identify potential therapeutic target proteins. Therefore, the current work provides useful biological material and opens a new range of opportunities for generating BSGFV-resistant banana plants through the genetic manipulation of the selected miRNAs. Full article

Functional foods are currently receiving increasing popularity in diet modification. Green bananas contain far more dietary fiber (DF) and resistant starch (RS) than mature bananas. The potential for integrating these vital components into food, such as bread, has expanded. Thus, this study aimed to examine the physicochemical and rheological behavior of wheat flour dough after the addition of varying amounts of Australian, green banana flour (GBF) substitutions (5, 10, 15, 25, and 30%). Using MixoLab 2, we recorded the rheological parameters of the dough that had GBF substitutions. Additionally, the flour color (‘L*’, ‘a*’, and ‘b*’ value) and crumb cell structure analysis were evaluated. Although increasing the amount of GBF replacement generally improved dough quality with all banana cultivars, GBF from Cavendish and Ladyfinger showed a greater improvement than Ducasse. Improved dough mixing stability and increased viscosity, starch gelatinization, and retrogradation were all predicted to contribute to longer bread shelf life. RS content of the enriched bread increased significantly with both Ladyfinger and Ducasse (2.6%), while Ladyfinger bread had the highest DF (9.1%). With increasing GBF, L*, a*, and b* values were changed considerably with a strong linear correlation. A MATLAB analysis indicated substantial variations across samples regarding the small, medium, and total air space counts based on 10% banana flour as a standard level of addition. In conclusion, the processing properties and nutritional value of wheat flour can be enhanced by replacing specific proportions of wheat flour with green banana flour without major detrimental effects on dough processing attributes and thus highlight the possibility of utilizing GBF from different banana varieties for use in fine-tuning composite flour developments. Full article