Search Results (2,196)

Recently, the role of mucosal intestinal microbiota in human health has received increasing attention. Nevertheless, data on the response of this microbiota to various interventions remain limited. Here, we have employed the Mucosal Simulator of Human Gastrointestinal Microbial Ecosystem (M-SHIME^®) and luminal SHIME^® (L-SHIME^®) to examine mucosal microbiota responses to interventions that are known to impact the intestinal microbial community in humans and study relationships between the responses of mucosal and luminal microbiota. Specifically, we evaluated the effects of varying macronutrient levels over a 28-day standard, balanced dietary intervention and a parallel 14-day administration of Lacticaseibacillus rhamnosus GG. Observed shifts in mucosal microbiota in response to interventions differed significantly from those observed in luminal microbiota (p < 0.05). In particular, we found that the mucosal microbiota compared to luminal microbiota was more stable and that the abundance of several genera (i.e., Subdoligranulum, Parabacteroides and Fusobacterium) in the M-SHIME^® correlated positively with the intake of dietary macronutrients, especially protein, which was in line with results reported in previous human studies. This study demonstrates the reliability of advanced in vitro models in capturing diet-induced dynamics of the human mucosal microbiota, a compartment that remains understudied despite its critical role in intestinal immune regulation. Full article

This study evaluated the optimal dietary lipid level for Atlantic salmon (Salmo salar) reared in freshwater, aiming to provide foundational knowledge for the development of cost-effective and nutritionally balanced aquafeeds. Four experimental diets were formulated to contain comparable crude protein levels (47%) but graded lipid levels of 14% (L14), 16% (L16), 18% (L18), and 20% (L20), and were fed to salmon with an initial mean body weight of 241.5 ± 9.7 g during a 12-week feeding trial. Fish in the L16 group exhibited the highest weight gain (WG) and feed efficiency (FE), whereas those in the L14 group showed significantly reduced growth performance. Antioxidant analysis revealed that glutathione peroxidase (GPx) activity was lowest in the L14 group (p < 0.05), while plasma glucose concentration was minimized in the L16 group (p < 0.05). Transcriptomic profiling of liver tissue from the L14 and L16 groups identified 2117 differentially expressed genes (DEGs). Genes associated with lipid metabolism were more highly expressed in the L16 group, whereas immune- and inflammation-related genes were upregulated in the L14 group. These findings suggest that a dietary lipid level of approximately 16% is most favorable for promoting growth, metabolic stability, and overall health in freshwater-reared Atlantic salmon, thereby providing practical guidance for optimizing feed formulation and improving the economic efficiency of freshwater salmon aquaculture. Full article

The valorization of cull sheep and the incorporation of agro-industrial by-products into animal feeding represent effective approaches to enhancing the sustainability of small ruminant production systems. This study investigated the effects of dietary inclusion of 17% partially destoned olive cake (OC) in the concentrate fed to Valle del Belice ewes on carcass characteristics, as well as on meat and salami quality. A 14-week feeding trial was conducted on 124 animals allocated to a control (CTR) and an experimental (EXP) group, balanced for parity, days in milk, and daily milk yield. At the end of the trial, five animals per group were slaughtered and their meat was processed into three types of salami: 100% sheep meat (SM), 90% sheep meat with 10% beef heifer brisket (HB), and 90% sheep meat with 10% pork backfat (PB). Meat and salami were evaluated for chemical composition, fatty acid profile, polyphenol content, antioxidant capacity, lipid oxidation, microbiological status, textural properties, and sensory characteristics. Dietary OC supplementation resulted in increased carcass weight, separable fat, intramuscular fat content, and monounsaturated fatty acids—particularly oleic acid—along with higher polyphenol levels and antioxidant activity. Salami produced from OC-fed ewes exhibited reduced weight loss during ripening, lower lipid oxidation, an improved MUFA/SFA ratio, and satisfactory sensory attributes. Microbiological analyses indicated a dominance of lactic acid bacteria and coagulase-negative staphylococci, with no pathogenic microorganisms detected. Overall, the inclusion of olive cake in the diet enhanced meat and processed product quality, supporting the valorization of olive oil by-products within circular economy frameworks. Full article

In the face of escalating environmental crises, sustainable development is becoming one of the key global challenges. A growing body of research indicates that transitioning to plant-based diets, including a vegan diet, can significantly improve environmental sustainability. Young people from Generation Z play a crucial role in shaping future consumption patterns, and their beliefs and attitudes toward sustainable food may serve as an important indicator of social changes in the area of nutrition and the potential for implementing pro-ecological practices. The aim of the study is to assess students’ perceptual attitudes toward plant-based products, which constitute an integral part of vegan diets, addressing a significant gap in the literature on sustainable consumption among Generation Z in emerging economies. The study allows for the identification of the main sources of information, psychosocial barriers, and motivational factors underlying the adoption of a vegan diet, situating them within sustainable development paradigms. The research group consisted of 263 university students in Warsaw (Poland). A CAWI survey method was used, with a proprietary questionnaire. The study shows that more than 60% of respondents were not interested in this dietary option, and a similar proportion did not expect to adopt veganism in the future. Students’ attitudes toward the vegan diet are shaped primarily by their self-assessment of nutritional knowledge, previous experiences with plant-based diets, and socio-cultural factors. Although many respondents recognize the health and environmental benefits of a plant-based diet, the lack of reliable knowledge, concerns about deficiencies, higher product costs, and social pressure remain key barriers limiting their willingness to change eating habits. From a practical perspective, the findings highlight the need to develop nutrition education focused on the competencies required to properly balance plant-based diets, to increase the economic and sensory accessibility of vegan food, and to counteract persistent stereotypes and misconceptions about veganism, especially on social media, which is the main source of information for this age group. Full article

Pregnancy represents a period of heightened oxidative demand in which maternal metabolic adaptations are tightly regulated by redox-sensitive molecular pathways. Imbalances in these systems have been associated with gestational complications, impaired placental function, and long-term effects on offspring health. This review examines the molecular mechanisms through which adherence to the Mediterranean diet (MD) influences oxidative balance during pregnancy. We summarize evidence on how MD-derived bioactives regulate oxidative stress pathways and affect oxidative stress biomarkers, as well as the expression of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase. At the same time, certain MD foods containing environmental contaminants may potentially attenuate its protective effects. In addition, the review explores molecular insights into how the MD may counteract oxidative stress induced by environmental pollutants through modulation of redox signaling and detoxification pathways. By integrating biochemical, molecular, and environmental perspectives, this review highlights the MD as a potential nutrigenomic intervention to optimize oxidative balance, support healthy pregnancy outcomes linked to environmental pollution. Full article

Background/Objectives: Youth with type 1 diabetes (T1DM) face unique challenges in balancing dietary choices, physical health outcomes, and social–emotional well-being in school settings. This cross-sectional exploratory pilot study examined the associations of diet with physical health and teacher-reported social–emotional functioning in students with T1DM. Methods: Students with T1DM (mean age = 13.42; 47 female, 50 male; 50% White, Non-Hispanic, 50% minority) self-reported their nutritional habits using the KBlock Dietary Screener for Children when school was in session. Teacher-rated school-related behaviors were assessed through the Behavior Assessment Scale for Children-2nd Edition (BASC-2). Canonical correlation analysis was conducted to determine whether the variable sets (diet with physical health and school-related behavioral health) shared a significant multivariate relationship. Results: Youth with lower glycemic loads and consuming more sugar, dairy, and meat/poultry/fish but fewer legumes, fruit, and less saturated fat exhibited fewer externalizing symptoms and higher BMI. Diet uniquely accounted for modest variance in combined social–emotional and physical health, controlling for demographics and T1DM duration. Findings support increasing the availability of whole, nutrient-rich foods, integrating comprehensive nutrition education into curricula, and ensuring access for all students, regardless of socioeconomic status. Conclusions: Comprehensive dietary assessments and school-based randomized control trials are needed to enact more evidence-based dietary recommendations or interventions for youth, aiming for a balanced approach that addresses both mental and physical health outcomes. Full article

Background: The increasing prevalence of low-birth-weight (LBW) offspring from obese mothers underscores the need for dietary strategies to mitigate the transgenerational propagation of metabolic diseases. Objectives: We determined whether dietary protein restriction under obesogenic conditions altered maternal energy balance and led to LBW offspring and whether branched-chain amino acid (BCAA) supplementation improved maternal energy balance and mitigated weight and craniofacial skeletal deficits in offspring. Methods: High-fat-fed obese pregnant Sprague Dawley rats (~8–10 weeks of age, n = 8–11/group) were randomized in study 1 to control high-fat diet (20% protein; HFD), low-protein diet (LP; 5% protein), and LP + BCAA diet (100% BCAA requirements) and in study 2 to control HFD (20% protein), LP (10% protein), and LP + 2BCAA diet (200% BCAA requirements). Post-weaning offspring were fed HFD until 8 weeks of age. Results: Protein restriction promoted hyperphagia and energy expenditure, whereas BCAA supplementation attenuated such hyperphagic effects in pregnancy but not in lactation. Protein restriction reduced maternal body weight in lactation, and although BCAA supplementation did not reverse the weight loss, it enhanced insulin sensitivity and paradoxically reduced offspring survival. Maternal protein restriction reduced offspring body weight and craniofacial bone growth that persisted into adulthood, but BCAA supplementation did not rescue such deficits. Conclusions: Maternal protein restriction in obese dams enhanced maternal energy expenditure but impaired offspring growth and development. Although BCAA supplementation improved maternal energy balance, it was insufficient to reverse the adverse effects of maternal protein restriction on offspring growth under obesogenic conditions. Full article

Diet plays a pivotal role in shaping the gut microbiota, influencing host physiology, immune function, and nutrient metabolism. In this study, we evaluated the impact of three distinct feeding systems—Forage only, Balanced feed only, and Mixed system—on the cecal microbiota of guinea pigs (Cavia porcellus) using 16S rRNA gene amplicon sequencing in a randomized allocation of 18 males across the three diets (n = 6 per group) over 7 weeks. A total of 2,135,852 high-quality reads were obtained, with rarefaction curves and Good’s coverage confirming sufficient sequencing depth. Alpha diversity indices revealed significantly higher microbial richness and evenness in the mixed group, while beta diversity analyses demonstrated distinct microbial community structures across diets. Taxonomic profiling showed that forage-based diets enriched fiber-degrading genera such as Fibrobacter and Treponema, whereas the Balanced feed group favored mucin- and protein-degrading bacteria like Akkermansia and Bacteroides. LEfSe and t-test analyses identified several biomarkers and diet-specific genera, suggesting functional divergence in microbial metabolism. Forage-fed animals showed microbiota associated with short-chain fatty acid production and enhanced fiber utilization, while the Balanced feed group showed microbial traits linked to mucin degradation and potential gut barrier disruption. These findings highlight the strong influence of dietary composition on gut microbial ecology and suggest that fiber-rich diets promote a more diverse and functionally beneficial cecal microbiome in guinea pigs. Full article

Background: Gastrointestinal discomfort affects up to 70% of individuals with spinal cord injury (SCI), largely due to gut dysbiosis caused by altered transit time and reduced gastrointestinal motility from autonomic disruption. Emerging evidence links prebiotics and probiotics to improved microbiome balance and reduced inflammation, yet data in SCI remain limited. Methods: Individuals aged ≥ 18 years, with a chronic SCI (≥1 year) experiencing significant gastrointestinal symptoms, will be invited to participate in this single-center randomized controlled crossover trial. Persons currently taking antibiotics, who have relevant eating or digestive disorders, or who have undergone a recent diet change will be excluded from the study. Participants will be randomized (1:1) into two groups. The first group will take a probiotic (Biotics-G, Burgerstein AG, Rapperswil-Jona, Switzerland) supplement for eight weeks, then after a four-week washout period, they will take a prebiotic (Oat Bran, Naturaplan, manufactured by Swissmill, Zurich, Switzerland) supplement for another eight weeks. The second group will receive the supplements in reverse order. The primary outcome is the Gastrointestinal Quality of Life Index, a questionnaire to assess quality of life related to gastrointestinal disorders. Secondary outcomes consist of gastrointestinal transit time, inflammatory blood markers, and gut microbiome composition. Ethics: The study will be conducted in accordance with the Declaration of Helsinki. The study was approved by the Ethics Committee for Northwest/Central Switzerland (EKNZ, ID: 2025-00238, 24.02.2025, Version 2.0). The study is registered at ClinicalTrials.gov (ID: NCT06870331, 02.04.2025). Written informed consent will be obtained from all participants involved in the study. Full article

Non-alcoholic fatty liver disease (NAFLD) is a major metabolic disorder characterized by hepatic lipid accumulation, oxidative stress, and disturbance of lysosomal degradation. Central to these processes is glutathione (GSH), a key antioxidant regulating redox balance and cellular homeostasis. This study aimed to evaluate the therapeutic potential of two dietary antioxidants—astaxanthin and Garcinia indica (kokum)—in modulating hepatic redox status, lysosomal function, and metabolic gene expression in a murine model of diet-induced NAFLD. A total of 120 male Swiss Webster mice were allocated into control and steatotic groups, followed by a 90-day supplementation period with astaxanthin, kokum, or their combination. Liver tissue was collected post-supplementation for biochemical, antioxidant, and qRT-PCR analyses. Outcomes included lysosomal enzymes activities, superoxide dismutase (SOD), GSH, vitamin C, total polyphenols, DPPH radical-scavenging activity, and total antioxidant capacity (TAC). NAFLD induced marked oxidative stress, lysosomal overactivation, and alteration of antioxidant-related gene expression. Combined supplementation restored GSH, enhanced TAC, reduced lysosomal stress markers, and significantly upregulated nuclear factor erythroid 2-related factor 2 (Nfe2l2) while downregulating fatty acid synthase (FASN) and partially rescuing lipoprotein lipase (LpL). Correlation analyses revealed strong associations between antioxidant capacity, lysosomal function, and transcriptional regulation, supporting the therapeutic relevance of combined antioxidant therapy for concurrent redox and lysosomal dysregulation in NAFLD. These findings underscore the therapeutic potential of targeting redox and cellular degradation pathways with antioxidant-based interventions to re-establish hepatic metabolic balance in NAFLD and related disorders. Full article

Gut microbiota has emerged as a modulator of host metabolism and energy balance. However, the precise microbial metabolites mediating thermogenic activation in obesity remain largely undefined. We investigated the effect of antibiotic treatment under a high-fat diet on metabolites and its contribution to lipolysis and thermogenesis. Antibiotic treatment in high-fat diet-fed rats reduced adiposity and enhanced adaptive thermogenesis. Metabolomics revealed elevated taurine levels in the cecum content and plasma of antibiotic-treated animals, correlating with increased expressions of UCP1 and TGR5 in brown adipose tissue. Taurine enhanced lipolysis and oxygen consumption in mouse adipose tissue and human adipocytes. Thereby, taurine modulated lipolysis dependent on TGR5 signaling in adipose tissue. Human data confirmed that taurine promotes browning of white adipocytes and that acute cold exposure leads to a marked drop in circulating taurine, suggesting its rapid recruitment into thermogenic tissues. Besides its synthesis in the liver and dietary uptake, taurine can be a microbiota-derived metabolite that activates adipose thermogenesis and lipolysis through TGR5 and possibly taurine transporter-dependent mechanisms. These findings uncover a gut–adipose axis with therapeutic potential for metabolic disease. Full article

The effects of pelleting a total mixed ration (TMR) for dairy sheep were investigated in an experiment involving 24 lactating Assaf ewes, which were assigned to two groups and fed the same TMR ad libitum, offered either in pelleted (PTMR group, n = 12) or in unpelleted form (CTMR group, n = 12). The experiment lasted 28 days, during which feed intake, eating behavior (including meal frequency and size, meal duration, eating rate, between-meal interval), and milk yield were recorded daily. Body weight (BW) was recorded on days 1 and 28 and milk samples were collected on days 1, 8, 15, 22 and 28 for milk composition analysis. Blood acid-base status was determined at the beginning and at the end of the trial. Ewes fed the CTMR diet exhibited (p < 0.05) a higher meal frequency and longer meal duration, along with a smaller meal size and slower eating rate. However, feed intake in this group was less than that in ewes fed PTMR only during the final two weeks of the experimental period. Total eating time was also longer (p < 0.001) in the CTMR group, whereas the average time between meals was shorter (p < 0.002). No differences (p > 0.05) were observed between dietary treatments in blood acid-base status, milk yield or milk composition. However, a diet x day interaction (p < 0.05) was detected for milk yield, as during the last 2 weeks of the experimental period the ewes fed the PTMR yielded more milk than those fed the CTMR. Feed conversion ratio did not differ between groups (p > 0.05), but body weight loss was greater in ewes fed the CTMR diet (−3.00 vs. −0.58 kg; p < 0.05). A trend toward improved feed efficiency was observed in the PTMR group when calculated based on milk yield corrected for that theoretically derived from the mobilization of body reserves (1.98 vs. 1.41 g DMI/kg milk; p = 0.077), with estimated contributions from body reserves of 485 g/day in the CTMR group and 70 g/day in the PTMR group. In conclusion, the use of pelleted total mixed rations in high-yielding dairy ewes enhances feed intake, feed efficiency, milk yield, and energy balance without adversely affecting milk composition or animal health in the short term. Full article

Background: Chronic kidney disease (CKD) represents a state of persistent, sterile low-grade inflammation in which sustained innate immune activation accelerates renal decline and cardiovascular complications. Diet-induced gut dysbiosis and intestinal barrier dysfunction lower mucosal immune tolerance, promote metabolic endotoxemia, and position the gut as an upstream modulator of systemic inflammatory signaling along the gut–kidney axis. Scope: Most studies address microbiota-derived metabolites, food-derived bioactive peptides, or omega-3 fatty acids separately. This review integrates evidence across these domains and examines their convergent actions on epithelial barrier integrity, immune polarization, oxidative-inflammatory stress, and inflammasome-dependent pathways relevant to CKD progression. Key mechanisms: CKD-associated dysbiosis is characterized by reduced short-chain fatty acid (SCFA) production and increased generation and accumulation of uremic toxins and co-metabolites, including indoxyl sulfate, p-cresyl sulfate, trimethylamine N-oxide, and altered bile acids. Reduced SCFA availability weakens tight junction-dependent barrier function and regulatory immune programs, favoring Th17-skewed inflammation and endotoxin translocation. Bioactive peptides modulate inflammatory mediator networks and barrier-related pathways through effects on NF-κB/MAPK signaling and redox balance, while omega-3 fatty acids and specialized pro-resolving mediators support resolution-phase immune responses. Across these modalities, shared control points include barrier integrity, metabolic endotoxemia, oxidative stress, and NLRP3 inflammasome activation. Conclusions: Although evidence remains heterogeneous and largely preclinical, combined nutritional modulation targeting these convergent pathways may offer greater immunomodulatory benefit than isolated interventions. Future multi-omics-guided, factorial trials are required to define responder phenotypes and translate precision immunonutrition strategies into clinical CKD care. Full article

Understanding how primary structural features and secondary material properties adapt to functional loads is essential to determining their effect on changes in joint biomechanics over time. The objective of this study was to map and correlate spatiotemporal changes in primary structural features, secondary material properties, and dentoalveolar joint (DAJ) stiffness with age in rats subjected to prolonged chewing of soft foods versus hard foods. To probe how loading history shapes the balance between the primary and secondary features, four-week-old rats were fed either a hard-food (HF, N = 25) or soft-food (SF, N = 25) diet for 4, 12, 16, and 20 weeks, and functional imaging of intact mandibular DAJs was performed at 8, 12, 16, 20, and 24 weeks. Across this time course, the primary structural determinants of joint function (periodontal ligament (PDL) space, contact area, and alveolar bone socket morphology) and secondary material and microstructural determinants (tissue-level stiffness encoded by bone and cementum volume fractions, pore architecture, and bone microarchitecture) were quantified. As the joints matured, bone and cementum volume fractions increased in both the HF and SF groups but along significantly different trajectories, and these changes correlated with a pronounced decrease in PDL-space from 12 to 16 weeks in both diets. With further aging, older HF rats maintained significantly wider PDL-spaces than SF rats. These evolving physical features were accompanied by an age-dependent significant increase in the contact ratio in the SF group. The DAJ stiffness was significantly greater in SF than HF animals at younger ages, indicating that food hardness-dependent remodeling alters the relative contribution of structural versus material factors to joint function across the life course. At the tissue level, volumetric strains, representing overall volume changes, and von Mises bone strains, representing shape changes, increased with age in HF and SF joints, with volumetric strain rising rapidly from 16 to 20 weeks and von Mises strain increasing sharply from 12 to 16 weeks. Bone in SF animals exhibited higher and more variable strain values than age-matched HF bone, and changes in joint space, degrees of freedom, contact area, and bone strain correlated with joint biomechanics, demonstrating that multiscale functional biomechanics, including bone strain in intact DAJs, are colocalized with anatomy-specific physical effectors. Together, these spatiotemporal shifts in primary (structure/form), and secondary features (material properties and microarchitecture) define divergent mechanobiological pathways for the DAJ and suggest that altered loading histories can bias joints toward early maladaptation and potential degeneration. Full article

Acetaminophen (APAP) overdose is a major cause of acute liver failure and can be fatal, often without early symptoms. Protein deficiency, arising from illness or inadequate diet, impairs growth, immunity, and tissue repair. Both conditions can harm the kidneys, yet the impact of energy imbalance on renal physiology remains unclear. In this study, APAP toxicity and a low-protein diet induced behavioral suppression and tissue damage, as evidenced by reduced whole-body, liver, and kidney weights in rats. In kidney mitochondria of rats exposed to only toxic APAP doses, ATP levels declined sharply while ADP and AMP increased. AMP deaminase and ATPases’ activities rose about twofold and 1.5-fold, respectively, whereas cytosolic 5′-nucleotidase activity fell nearly threefold, suggesting compensatory responses to disrupted energy balance. The strongest reductions in ATP and the greatest increases in AMP and ATPase activity occurred in APAP-intoxicated rats fed a low-protein diet. This combination also intensified lipid peroxidation and oxidative protein damage, evidenced by elevated TBARS, reduced protein SH-groups, and increased protein carbonyls. Overall, APAP intoxication with protein deficiency disrupts renal energy metabolism, leading to mitochondrial dysfunction and structural kidney injury. Nutritional status therefore critically influences drug-induced nephrotoxicity, and antioxidant strategies may help prevent damage under metabolic stress. Full article