Search Results (1,502)

Objectives: This study aimed to develop curcumin nanoparticles (Cur@PCL-PEG-MF/cRGDfc) with retinal-targeting capability and to evaluate their biological effects and pharmacological mechanisms in vitro. Methods: After synthesis of the carrier framework, metformin (MF) and cRGDfc were conjugated to the carrier material using the carbodiimide method and Michael addition reaction, respectively. Subsequently, self-assembled nanoparticles were formed from the carrier and curcumin under specific conditions. The materials were characterized by spectroscopy, chromatography, elemental analysis, energy-dispersive spectroscopy and X-ray diffraction. The efficacy of the formulation was evaluated in two cell lines, ARPE-19 and HUVEC-T1. In addition, the pharmacological mechanism was explored using transcriptome sequencing as a complementary approach. Key Findings: Self-assembled nanoparticles were successfully prepared by combining the two modified carrier materials, PCL-PEG-MF and PCL-PEG-cRGDfc, with curcumin. The nanoparticles exhibited an encapsulation efficiency of 78.09%, a particle size of 162.33 nm, and a zeta potential of −23.28 mV and displayed a spherical morphology. They showed sustained release in simulated physiological conditions and stronger affinity for ARPE-19 cells under oxidative stress. Nearly 100% of the nanoparticles were internalized by the cells, which was accompanied by reduced ROS and LDH release and decreased DNA fragmentation. In addition, the nanoparticles inhibited neovascularization by reducing VEGF-A release, thereby potentially protecting the retina in macular degeneration and reducing choroidal hemorrhage. Further analyses showed that curcumin and its nanoformulations significantly reduced the expression of inflammatory factors such as IL-1β and IL-18, lowered the protein levels of Caspase-1, GSDMD-N, and NLRP3, and increased AMPK levels. Conclusions: Using PCL-PEG as the carrier framework, MF and cRGDfc were conjugated to construct a curcumin-loaded nanoparticle with retinal-targeting capability. This nanoparticle, characterized by a small particle size, sustained release, and targeted delivery to retinal pigment epithelium (RPE) cells under oxidative stress, alleviated oxidative stress-induced damage. Its therapeutic effect may be mediated, at least in part, by interference with the AMPK/mTOR pathway and activation of the NLRP3/Caspase-1/GSDMD pathway. Full article

Flexible polyurethane (PUR) foams and latex rubber foams are widely used in furniture and mattress cushioning, yet conventional standardized mechanical tests only partially capture comfort-relevant behavior, particularly in layered constructions where material interactions and sequencing can alter elastic response. This study aimed to compare the mechanical (elastic) properties of selected three-layer composites of approximately 60 mm thickness (composed of conventional PUR, high-resilience PUR, low-resilience PUR, and latex foam) and to preliminarily assess whether combining foam types improves support of such setup and whether changing layer order modifies elasticity and support. Indentation hardness testing of multilayer cushions was conducted by ISO 2439:2008 Method E. Six three-layer systems (Alpha–Zeta) were assembled in two groups. Group X showed nearly identical support factors (2.6–2.7), high recovery (64.3–66.2%), low hysteresis loss (24.3–24.5%), and overlapping force–indentation (IFD) curves, indicating minimal effect of layer order and dominance of the PUR layers. Group Y exhibited higher but more sequence-dependent support (3.1–3.7), markedly reduced, wider range recovery (30.0–45.9%), increased hysteresis (33.0–34.7%), and more dispersed IFD curves. Placing high-resilience foam at the top partially improve recovery, whereas locating low-resilience foam at the surface increase energy loss. The research contributes in part to the body of knowledge about the behavior of the tested materials according to standardized rules. These preliminary results can be compared with other research findings and used in the preparation of testing models for multilayer foam composites, thereby generating new knowledge to improve the design of future experiments, which will result in increased sitting and lying comfort. Full article

In order to optimize the sealing structure of the electrolytic cell for hydrogen production by electrolysis of water and enhance its sealing performance, a finite element model of the electrolytic cell sealing was established using software. The influence of different parameters of the sealing rib structure on the sealing performance was studied, and the variation law of gasket compressive stress under different sealing rib slot widths, angles, and spacings was explored. The results show that under the material constants of C10 = 7.0 × 10⁻³ and C01 = 6.05 in the Mooney–Rivlin constitutive model of the gasket, the gasket will deform and embed into the sealing rib groove after compression. At the same time, two parts of stress concentration will occur at the contact area between the gasket and the sealing rib groove, namely tensile stress concentration and compressive stress concentration. This stress concentration is the main source of sealing effect in practical work. After adding the sealing rib groove, the contact area between the sealing rib area and the gasket increases. When maximizing the peak sealing compressive stress serves as the optimization criterion, the optimal pitch settles at 0.4 mm; if the optimization objective shifts to attaining the utmost contact area, the preferable spacing amounts to 1 mm, accompanied by a maximum contact area increment of 34.31 percent. After comprehensive deliberation over sealing stress magnitude, functional sealing area, gas tightness efficiency as well as practical engineering applicability, 0.8 mm is pinpointed in this dissertation as the globally optimal spacing dimension. With a sealing rib pitch of 0.8 mm, a breadth of 1 mm, and an inclined angle of 20 degrees, the gasket yields substantial sealing stress alongside optimized post-assembly sealing contact area, wherein 26.44 percent of the overall gasket area contributes to effective sealing performance. Full article

As part of efforts to identify genes associated with Trichomonas vaginalis resistance to 5-nitroimidazole drugs, thirty cryopreserved T. vaginalis isolates were revived and grown using Diamond’s TYM medium. Minimum lethal concentrations (MLCs) for metronidazole (MTZ), tinidazole (TDZ), and secnidazole (SEC) were determined using a drug susceptibility assay. Transcriptome profiling was performed for 15 MTZ-sensitive (MTZ-S, MLC < 50 µg/mL) and 15 MTZ-resistant (MTZ-R, MLC ≥ 50 µg/mL) isolates using next-generation RNA sequencing. Bioinformatics analyses identified differentially expressed genes (DEGs). Among the MTZ-R isolates, six exhibited low MLCs of 50 µg/mL, five had intermediate MLCs between 100 and 200 µg/mL, and four had high MLCs ≥ 400 µg/mL. Differential gene expression analysis identified 28, 140, and 73 significantly altered genes in low-, intermediate-, and high-level MTZ resistance groups, respectively, with predominantly upregulated expression patterns. The SEC-resistant (SEC-R) isolates exhibited 136 differentially expressed genes, whereas the TDZ-resistant (TDZ-R) isolates showed minimal transcriptional changes. Focused analyses of iron transport pathways revealed reduced expression of ZIP-family iron import genes, particularly TvZIP4 (TVAG_273550), the strongest predictor of resistance in elastic-net modeling (AUC = 0.795). Resistant isolates also demonstrated coordinated upregulation of iron–sulfur cluster assembly and hydrogenosomal protein-import pathways. Weighted gene co-expression network analysis (WGCNA) identified multiple resistance-associated transcriptional modules correlated with MTZ and SEC MLCs. A comparative transcriptomic–proteomic analysis revealed concordant upregulation of iron–sulfur cluster machinery but discordant regulation of hydrogenosomal cargo proteins, likely supporting a post-transcriptional restriction model. These findings provide a broader mechanistic framework for understanding 5-nitroimidazole resistance in T. vaginalis and identifying candidate biomarkers and pathways that may support future therapeutic and diagnostic development. Full article

At present, although there are many SCARA manipulator solutions with vertical lifting functionality, they generally suffer from high maintenance costs and complex structures. Moreover, systematic performance evaluations based on international standards are lacking, leading to unclear critical performance boundaries such as accuracy and payload in practical applications. To address these issues, this paper designs and manufactures a low-cost SCARA manipulator for educational and research demonstrations as well as light-duty electronic parts assembly scenarios. A “leadscrew + stepper motor” scheme is adopted for vertical lifting, and an Arduino Mega 2560 development board serves as the core controller, significantly reducing system cost. A three-dimensional model is established using SolidWorks 2022, and kinematic simulations are carried out with MATLAB 2024a to preliminarily verify the feasibility of the mechanism. Subsequently, a physical prototype is built and experimental tests are conducted in accordance with the ISO 9283 standard. The experimental results show that the repeatability of the manipulator is controlled within the range of 0.05–0.3 mm, the path deviation caused by vibration lies between −0.52 mm and 0.3 mm, and the maximum payload capacity is 3.91 N. These experimental data can serve as a benchmark for the design and performance comparison of similar low-cost manipulators. Full article

This paper presents a practical implementation of a 3D-printed spherical Luneburg lens for gain enhancement of a WR-28 open-ended waveguide antenna operating in the Ka-band. The lens is designed based on Luneburg theory and realized using a six-zone discretized gradient-index structure, providing a balance between theoretical performance and fabrication feasibility. The proposed design enables the realization of the required permittivity distribution using a single dielectric material, where the effective permittivity of each zone is controlled through infill variation in a fused deposition modeling (FDM) process. To facilitate fabrication, the lens is divided into two hemispherical parts, enabling reliable manufacturing and assembly while maintaining the intended dielectric profile. The antenna performance is experimentally evaluated through reflection coefficient (S₁₁) measurements and radiation pattern characterization in both the XZ and YZ planes over the frequency range of 26.5–40 GHz, including co-polarized and cross-polarized responses. The proposed antenna achieves a simulated realized gain ranging from 17.6 dBi to 19.83 dBi, while the measured realized gain ranges from 16.42 dBi to 18.43 dBi, with a maximum deviation of 1.47 dB. In comparison, the standalone WR-28 open-ended waveguide exhibits a measured realized gain of 7.22–8.01 dBi. The integration of the six-zone Luneburg lens results in a realized gain enhancement of 9.20–10.97 dB across the operating band. These results confirm that the proposed approach provides a simple, low-cost, and experimentally validated solution for high-gain millimeter-wave antenna applications, while maintaining good agreement between simulation and measurement. Full article

The European 2035 decarbonisation framework rests on a conditional premise—that higher renewable-electricity penetration accelerates battery electric vehicle (BEV) adoption—yet it has not been tested at the panel level. The question is timely: the December 2025 Automotive Package would soften the 2035 target from 100 to 90 percent CO₂ reduction and permit continued production of plug-in hybrids beyond 2035, while the Alternative Fuels Infrastructure Regulation (AFIR) imposes binding charging-coverage targets from 2025 onwards. We assemble an annual panel of 31 European economies over 2015–2024 (310 country-year observations) and combine a two-way fixed-effects baseline on five disaggregated powertrain shares, an interaction model with public charging coverage as a moderator, and a Hansen-style threshold panel. The within-country BEV-share coefficient on renewable-electricity penetration is statistically null (β = +0.18, p = 0.247), rejecting the linear premise. The plug-in hybrid share, by contrast, responds positively and unconditionally (β = +0.36, p = 0.001)—a “PHEV paradox” of compositional response. The BEV channel, by contrast, is conditional on infrastructure: its marginal effect rises with public charging coverage and is positive only in the upper part of the charging distribution (interaction β₃ = +0.13, p = 0.027). A formal Hansen-style threshold test in the renewable share does not reject the linear specification (sup-F = 0.73, bootstrap p = 0.97), so the BEV conditionality is identified through the charging-coverage interaction. The findings characterise a two-speed European transition. The first channel reflects compliance-led PHEV hedging; the second reflects BEV charging network complementarity enabled by AFIR-mandated coverage. Subsidy rebalancing away from PHEV eligibility, strict AFIR enforcement, and PHEV utility-factor reform are necessary policy levers for the 2035 framework to deliver full electrification rather than the partial electrification that current incentives yield. Full article

Micro and nanoplastics (MNPs) are increasingly recognized as contaminants in food systems; however, the specific packaging elements responsible for particle release remain poorly resolved. Most studies treat packaging as a single material category, without covering distinct contributions from the different units of modern food contact materials (FCMs). We propose a packaging structure taxonomy based on functional elements: container (C), closure (CL), and functional layers (F), including operational interfaces (+I), designed to enable components attribution of possible origins of plastic fragments in foods and beverages. Through a structured synthesis of the current literature, we map the primary processes leading to MNP generation across these modules, including tribological abrasion at closure contact interfaces, thermally driven polymer degradation in containers and delamination or shedding from coatings, adhesives and multilayer structures. Available evidence indicates that repeated mechanical actions such as opening and closing cycles can generate measurable particle release from closure assemblies. The proposed C/CL/F + I framework introduces standardized descriptors and reporting units that improve comparability across studies and supports origin attribution. By explicitly separating packaging parts and their operational interaction zones, the taxonomy provides a methodological bridge between analytical microplastic detection and engineering strategies aimed at minimizing particle formation. Its adoption can facilitate harmonized experimental design, strengthen regulatory risk assessment and guide the development of packaging configurations that minimize plastic particle shedding into foods. Full article

This study provides the first updated checklist of Moroccan freshwater molluscs, synthesizing faunistic knowledge accumulated between 1795 and 2026. The dataset was primarily compiled from specialized literature, complemented by the authors’ expertise and recent field investigations. Taxonomic classifications at the family, genus, and species levels were revised in accordance with current systematic interpretations. In total, 106 freshwater molluscan species, belonging to 55 genera and 14 families, were documented. Chorological analysis reveals a clear dominance of Palearctic elements, particularly of Mediterranean affinity, alongside a high proportion of strictly endemic Moroccan taxa. Most of these endemics are associated with springs (crenobionts) and subterranean habitats (stygobionts), emphasizing the role of these environments as key centres of micro-endemism and diversification. A comprehensive database comprising 838 occurrence records was assembled, including GPS coordinates and sampling dates. The analysis identifies biodiversity hotspots mainly concentrated in the Mediterranean part of Morocco, particularly within the Middle Atlas Mountains, followed by the northeastern regions, where the highest species richness and citation rates were recorded. Despite this progress, significant gaps remain in the knowledge of Moroccan freshwater molluscs. Several regions still require further exploration, while ongoing threats—including pollution, habitat degradation driven by anthropogenic activities, global environmental change, and biological invasions—pose significant challenges. Addressing these gaps calls for intensified research efforts, including comprehensive field surveys, integrative taxonomic and molecular approaches, and long-term ecological monitoring. Overall, this study represents a significant step toward advancing the knowledge and conservation of freshwater molluscan diversity in Morocco. Full article

This study evaluates the mechanical behavior of bone-implant assemblies used in treating complex proximal humerus fractures, a clinical challenge due to the anisotropic nature of bone and variability in patient-specific conditions. The aim of this study was to compare the stability and stress distribution of three fixation methods: polyaxial locking plates, monoaxial locking plates, and intramedullary nails. Using 4th-generation composite humerus models, a four-part fracture (Neer IV) was simulated. The assemblies underwent axial compression testing using a universal testing machine, complemented by finite element analysis (FEA) and stereomicroscopy. The results indicate that while both plate types exhibited similar mechanical behavior—with stiffness values around 113–115 N/mm and failure initiated by plastic deformation of the implant—the intramedullary nail configuration demonstrated higher stiffness values under the tested experimental conditions (1084 N/mm), approximately 9.5 times higher than that of the plates. However, the nail assembly failed through brittle fracture of the bone rather than implant deformation. We conclude that while the intramedullary nail configuration demonstrated higher stiffness under the tested experimental conditions, its performance is heavily dependent on bone quality. In contrast, locking plates may provide a more gradual load-transfer behavior by transferring a greater proportion of the mechanical load to the implant, potentially making them more suitable for osteoporotic bone conditions, where reducing excessive stress concentration within the bone tissue may be beneficial. Full article

This paper addresses an integrated production–transportation scheduling problem in assembly workshops, encompassing the processes of part machining, material handling via handling resources, and final synchronous assembly. The finite buffer capacities of production resources can cause blocking, thereby reducing efficiency. Material handling resources are subject to different service area restrictions, and some share safety zones with production resources, preventing simultaneous processing. To address this, a mixed-integer programming model is formulated with makespan and total empty travel time as bi-objective optimization targets. Since the mixed-integer linear programming (MILP) model faces difficulties in solving medium- and large-scale instances, an improved memetic NSGA-II algorithm (IMNSGA-II) is proposed. The algorithm adopts a three-segment chromosome encoding and incorporates a VNS-SA local search mechanism within the global evolutionary framework of NSGA-II. Small-scale computational experiments using Gurobi are first used to verify the correctness of the model. Decoupling experiments further demonstrate the necessity of integrated optimization: compared with phased baseline methods, IMNSGA-II reduces makespan and empty travel time by approximately 10.16% and 12.33%, respectively. In ablation and comparative experiments, results based on hypervolume (HV) and inverted generational distance (IGD) show that the proposed method achieves better convergence, diversity, and overall Pareto front quality than multiple baseline algorithms. These experiments confirm the effectiveness of the proposed model and algorithm. Full article

Part surface topography is characterized by complex multi-scale and multi-feature coupling, and accurate topography modeling is essential for predicting assembly precision in high-performance mechanical systems. Gaussian Process Regression (GPR) offers a principled, probabilistic framework for surface modeling from sparse measurements, but its performance depends critically on kernel function selection. A fixed single kernel lacks the flexibility to represent surfaces that simultaneously exhibit smooth trends, periodic textures, and linear drift. To address this limitation, an adaptive composite kernel method is proposed. Initial GPR residuals are analyzed through statistical hypothesis tests and spectral decomposition to identify which geometric features are present; matching base kernels—Squared Exponential (SE), Periodic (PER), and Linear (LIN)—are then selected and combined additively or multiplicatively. Experiments on three representative synthetic surfaces show that the composite kernels reduce RMSE by up to 95.09% relative to the single SE kernel. Validation on a machined part confirms that the method successfully transfers to real measured data, achieving a 30.65% RMSE reduction and raising R² from 0.9536 to 0.9777. The results demonstrate that residual-analysis-driven kernel selection yields physically interpretable models with substantially improved reconstruction accuracy. Full article

We design and implement a low-cost, modular integrated optical and atomic force microscope (AFM) based on an optical pickup unit (OPU) capable of stable contact-mode operation. By exploiting the inherent fixed conjugate planes within the OPU, we overcome the imaging difficulties caused by unfixed focal planes. This allows for real-time optical observation of the AFM probe position and the relative tip-sample position during operation with an optical resolution of 1.5 μm. Furthermore, by optimizing the circuit design and scanning logic, we suppress the OPU lens drift and system noise on imaging, enabling stable contact-mode operation with a signal noise of less than 2 nm. Built with off-the-shelf low-cost mechanical and electronic components alongside a few custom 3D-printed parts, this system features low cost, easy assembly, and high expandability. Full article

Studying soil microbiomes is challenging because soil contains thousands of microbial species at vastly different abundances. The choice of sequencing method has a strong effect on which of these species are detected and how the community is described. Three approaches now dominate soil microbiome research: short-read 16S rRNA amplicon sequencing on Illumina platforms, full-length 16S sequencing on Oxford Nanopore Technologies (ONT) platforms (particularly the R10.4.1 flow cell), and long-read shotgun metagenomics. Each has distinct biases that shape the recovered community, yet researchers routinely select a method based on cost, understanding, or local expertise rather than on a clear knowledge of what each approach methodically over- or under-represents. Here, we review head-to-head benchmarking studies that have applied two or more of these methods to the same soil or directly comparable samples. We show that while long-read and short-read 16S approaches generally converge on dominant taxa and on between-sample differences, they disagree substantially on alpha diversity estimates, rare taxon detection, and the relative abundances of entire phyla. The R10.4.1 flow cell chemistry has narrowed but not eliminated the accuracy gap with Illumina, and shotgun metagenomics reveals systematic biases in both short and long-read assembly that depend on population diversity within the sample. We synthesise this evidence into an evidence-based decision framework tied to specific research questions and recognise the gaps in soil-specific benchmarking that limit current methods. Rather than asking which platform is “best,” we argue that method choice should be framed as an important part of study design, with the biases of the chosen method acknowledged and, where possible, controlled for. Full article

The orbital space ecosystem is undergoing significant change, with numerous initiatives focused on in-orbit services, assembly, and manufacturing. These initiatives are being developed globally, with ongoing studies in America, Asia, and Europe. As these technologies evolve, questions arise about their compatibility and interoperability, especially for long-term in-orbit operations. The Space USB project addresses these challenges by aiming to connect European partners involved in the emerging interconnection systems market for in-orbit services. Its goal is to improve the compatibility and interoperability of these systems across Europe, with perspectives for broader international application. As part of this project, a prototype has been developed to connect with the interconnection systems of three European partners. A test campaign was conducted to assess the prototype’s functionality, using a robotic system. Full article