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Microorganisms
Special Issues
State-of-the-Art Antimicrobial Agents and Resistance Research in the USA
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State-of-the-Art Antimicrobial Agents and Resistance Research in the USA

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: closed (31 March 2026) | Viewed by 1289

Special Issue Editor

Dr. Maria King

E-Mail Website
Guest Editor
Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX, USA
Interests: aerobiology; collection and analysis of bioaerosols; computational modeling of bioaerosol transmission; antibiotic resistance; environmental factors, molecular dynamics modeling of interactions; biolayer interferometry kinetics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue seeks to offer a comprehensive overview of the latest advancements in the field of antimicrobial agents and resistance within the USA. We invite American researchers from related fields in the USA to submit papers that highlight recent developments in this area. Contributions may include original research articles and comprehensive reviews. We also encourage the invitation of relevant experts and colleagues to contribute, ensuring a diverse and cutting-edge collection of work. This Special Issue aims to showcase the innovative research conducted in the USA and to provide a platform to advance the design and testing of antimicrobial agents and modeling of their interactions to combat antimicrobial resistance.

Dr. Maria King
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacteria
  • microbiome
  • biofilm
  • bioaerosols
  • antimicrobials
  • antimicrobial resistance (AMR)
  • antibiotics
  • antibiotic-resistant bacteria (ARBs)
  • antibiotic-resistant genes (ARGs)
  • membrane channels
  • ligands
  • receptors
  • molecular dynamics (MD) modeling
  • biolayer interferometry (BLI)

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Published Papers (1 paper)

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Research

13 pages, 436 KB  
Article
Frequency of Antimicrobial-Resistant Fecal Escherichia coli Among Small, Medium, and Large Beef Cow–Calf Operations in Florida
by Ahmad Ali, João H. J. Bittar, Lekshmi K. Edison, James Colee, Thomas Denagamage, Jorge A. Hernandez and Subhashinie Kariyawasam
Microorganisms 2026, 14(1), 13; https://doi.org/10.3390/microorganisms14010013 - 20 Dec 2025
Viewed by 998
Abstract
Antimicrobial resistance (AMR) is a growing threat to both animal and human health. This study investigated the occurrence of AMR in Escherichia coli isolated from fecal samples collected from beef cow–calf operations and compared the frequency of antimicrobial-resistant fecal E. coli among small, [...] Read more.
Antimicrobial resistance (AMR) is a growing threat to both animal and human health. This study investigated the occurrence of AMR in Escherichia coli isolated from fecal samples collected from beef cow–calf operations and compared the frequency of antimicrobial-resistant fecal E. coli among small, medium, and large beef cow–calf operations in Florida, United States. The study included nine beef cow–calf operations. Between December 2023 and April 2024, a total of 743 fecal samples were collected from cows (n = 429) and calves (n = 314), either directly from the rectum or from fresh voided feces. From these samples, 3475 E. coli isolates (five isolates/animal) were subjected to antibiotic susceptibility testing using the Kirby–Bauer disk diffusion method. A panel of eight antibiotics was used to assess AMR profiles. Irrespective of farm size, cows and calves showed higher resistance to streptomycin (47% or 330/695), oxytetracycline (46% or 319/695), sulfadimethoxine (42% or 291/695), ampicillin (41% or 283/695), and florfenicol (18% or 126/695). In contrast, lower resistance frequencies were observed for gentamicin (4% or 27/695), ceftiofur (3% or 19/695), and trimethoprim–sulfamethoxazole (3% or 18/695). Animals from medium (OR = 1.67) and large operations (OR = 2.70) had greater odds of harboring E. coli resistant to ≥1 antibiotic than those from small operations. Streptomycin (medium OR = 1.92; large OR = 4.17) and sulfadimethoxine resistance (medium OR = 1.64; large OR = 3.45) were also more frequent in medium and large operations, respectively. Additionally, E. coli resistance to florfenicol was higher in calves (OR = 3.57) than in cows, after controlling for farm size. This study provides new insights into AMR patterns in fecal bacteria from beef cow–calf operations in Florida and can help producers and veterinarians develop informed strategies for monitoring and mitigating AMR. Full article
(This article belongs to the Special Issue State-of-the-Art Antimicrobial Agents and Resistance Research in the USA)
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