Search Results (12,908)

Background/Objectives: Comparative studies examining postoperative pain and cosmetic outcomes following single-port laparoscopic appendectomy (SLA) and three-port laparoscopic appendectomy (TLA) in pediatric patients with appendicitis have produced inconsistent results. We aimed to determine whether SLA offers practical benefits over TLA in terms of recovery-phase pain relief and long-term cosmetic satisfaction in pediatric patients. Methods: This prospective comparative study included children aged 15 years or younger who underwent laparoscopic appendectomy for uncomplicated acute appendicitis. The degree of pain reduction was compared between the SLA and TLA groups on postoperative days (PODs) 1, 2, and 7, both at rest and during coughing and ambulation, using the Visual Analog Scale for Pain (VASP). Global cosmetic satisfaction was assessed at 1 month and 3 years postoperatively using the Visual Analog Scale for Cosmesis (VASC). Scar perception was evaluated with the Patient and Parental Scar Assessment Scale (PSAS). The primary outcome was the degree of pain reduction during ambulation on POD7. The secondary outcome was global cosmetic satisfaction at 3 years. Propensity score matching (PSM) was used as a sensitivity analysis to control for baseline differences. Continuous variables were assessed for normality using the Shapiro–Wilk test. Results: Baseline characteristics were similar among 238 patients (127 SLA and 111 TLA). SLA resulted in significantly greater pain reduction during ambulation on POD7 (deltaVASP7_walk: −6.22 ± 2.60 vs. −5.06 ± 3.23, p < 0.01, mean difference = −1.16, Cohen’s d = 0.39). However, this difference did not reach the minimal clinically important difference (MCID) threshold of 1.3. PSM analysis with 82 matched pairs confirmed the results, with even larger effect sizes. At 3 years, the SLA group reported significantly higher cosmetic satisfaction (VASC: median 10 [9–10] vs. 8 [6–9], p < 0.001, r = 0.44), surpassing the MCID of 1.5. The TLA group scored worse in scar perception regarding color, stiffness, thickness, and irregularity. Mediation analysis indicated that 66% of the overall effect on cosmetic satisfaction was mediated by scar perception. Conclusions: Although SLA offers statistically significant yet clinically marginal benefits in early postoperative pain reduction, it provides substantial benefits in long-term cosmetic satisfaction compared with TLA in pediatric patients. Full article

Brassinosteroid analogs with heterocyclic rings in the side chain are interesting because important biological activity has been shown by these compounds. Thus, herein, five 23-24-dinorcholane BR analogs with a heterocyclic ester function at C-22 were synthesized and fully characterized by different spectroscopic techniques. The acylation reaction at C-22, which is a key synthetic step, was carried out by two different methods, namely acylation with heterocyclic acid chlorides and Steglich esterification reaction. In both cases, the acyl derivatives were obtained with good yields. Additionally, a preliminary molecular docking study of BRI1–BAK1 complexes formed by these analogs and brassinolide was performed to estimate what their biological activity would be. Results indicate that the complex formed by the analog 36, which has an indole group in the side chain, within the active site of BRI1–BAK1 is more stable than that formed by brassinolide. Additionally, molecular docking of a derivative having a benzoate function at C-22 and a F atom in the ortho position, 23, shows a similar pose and interactions at the active site but the highest binding energy. As 23 has shown similar activity to brassinolide in the Rice Lamina Inclination Test, it is expected that 36 will also exhibit similar behavior. Full article

The growing demand for sustainable and nutritionally balanced protein sources has intensified global interest in edible insects as an emerging alternative to conventional animal- and plant-based proteins. This review synthesizes current knowledge on insect proteins with a clear focus on four dimensions: nutritional value, functional properties, bioactivities, and safety considerations. Edible insects such as Bombyx mori, Acheta domesticus (A. domesticus), Tenebrio molitor, and Hermetia illucens provide high-quality proteins rich in essential amino acids, with favorable digestibility and bioavailability. Their unique functional characteristics—including solubility, emulsification, foaming, and gelation—support versatile applications in food formulations ranging from meat analogs to protein-fortified products. Insect-derived peptides further exhibit diverse bioactivities, such as antioxidant, anti-hypertensive, antidiabetic, and antimicrobial effects, highlighting their potential as functional food ingredients. Nevertheless, allergenicity and consumer acceptance remain critical challenges that must be addressed through improved processing technologies and regulatory frameworks. By systematically integrating these perspectives, this review underscores the promise of insect proteins as future food and health resources while outlining key barriers and research priorities for their safe and sustainable utilization. Full article

Background: Lower limb malalignment is a hallmark of knee osteoarthritis, with surgical correction techniques evolving from traditional mechanical alignment (MA) to kinematic alignment (KA) approaches. Restrictive kinematic alignment (rKA) represents a hybrid strategy combining principles from both techniques. This study evaluated short-term functional outcomes following robotic-assisted total knee arthroplasty (RoTKA), comparing MA versus rKA alignment strategies. Methods: This prospective, randomized, single-center study enrolled 96 patients with grade 3–4 idiopathic knee osteoarthritis (Kellgren–Lawrence classification). Patients were randomized to MA (n = 49, mean age 67 ± 9 years) or rKA (n = 47, mean age 66 ± 7 years) groups. Preoperative hip–knee–ankle (HKA) angles were 172.6° ± 1.1° and 172.9° ± 0.9° for MA and rKA groups, respectively. Outcomes were assessed using Visual Analog Scale (VAS) pain scores, range of motion (ROM), Knee Society Score (KSS), Oxford Knee Score (OKS) (primary outcome), SF-36, and Forgotten Joint Score (FJS-12). Results: Postoperative HKA angles were 179.5° ± 1.2° (MA) and 176.0° ± 1.5° (rKA). At 14 days postoperatively, knee ROM increased by 20.5% in the MA group and 25.7% in the rKA group, with a statistically significant 5.2% intergroup difference, indicating faster postoperative recovery (p = 0.008). VAS pain scores decreased by 7% in the rKA group while increasing by 13% in the MA group (p < 0.001). At one-year follow-up, FJS-12 scores were significantly higher in the rKA group (94.8 ± 3.2 vs. 91.9 ± 2.2, p < 0.001). No significant differences were observed in KSS, OKS, or SF-36 score between groups. Conclusions: Restrictive kinematic alignment demonstrated superior early postoperative outcomes compared to mechanical alignment in RoTKA, with significantly reduced pain and improved ROM. While one-year functional outcomes were comparable between techniques, rKA may offer advantages in the immediate postoperative period, supporting its consideration as a viable alignment strategy in robotic-assisted knee arthroplasty. Full article

Building off previous work on π-extended nickel thiophenedithiolenes, a series of thiazole-fused nickel dithiolene complexes have been prepared via similar synthetic methods, thus allowing for the addition of aryl groups to the terminal α-position of the fused thiazoledithiolene unit. In addition to π-extended complexes incorporating thiophene, phenyl, and furan end-groups, the methyl-terminated species has also been prepared as a representative of the simple nickel thiazoledithiolene core. The optical, electronic, and structural properties of these complexes have been characterized, and comparisons to the analogous nickel thiophenedithiolenes show that the replacement of thiophene by thiazole stabilizes the frontier orbitals of the thiazole-based complexes, while preserving the planar geometry, electronic delocalization, and low-energy NIR absorption of the previous nickel thiophenedithiolene species. Full article

Kappa opioid receptor (KOR) antagonists may have therapeutic potential to prevent stress-induced relapse in abstinent individuals with cocaine use disorder (CUD). The macrocyclic peptide [D-Trp]CJ-15,208 (cyclo[Phe-D-Pro-Phe-D-Trp]) is an orally bioavailable, brain–penetrant selective KOR antagonist that prevents stress-induced reinstatement of cocaine-seeking behavior in a mouse model of CUD. We synthesized and evaluated analogs of this lead compound with substitutions for the D-Trp residue to identify analogs that exhibit more potent central KOR antagonism following oral administration. The peptides were synthesized by a combination of solid phase and solution peptide synthetic methodologies, and their pharmacological activity was evaluated both in vitro (for KOR affinity, selectivity and antagonism) and in vivo (for antinociception and KOR antagonism), with promising analogs evaluated for their ability to prevent stress-induced reinstatement of cocaine-seeking behavior in the mouse conditioned place preference (CPP) assay. A variety of substituted D-Phe or modified D-Trp derivatives were tolerated by KOR with retention of significant KOR antagonism in vivo after oral administration. Macrocyclic peptide pretreatment, per os, significantly prevented stress-induced reinstatement of cocaine CPP at doses of 10 and 30 mg/kg of [D-Phe⁴]CJ-15,208, 4, and 30 mg/kg of [D-Trp(formamide)]CJ-15,208, 3, which are 6-fold and 2-fold lower, respectively, than that needed for {D-Trp]CJ-15,208. Full article

This paper presents a systematic approach to the development and validation of a monitoring and protection system based on the IEC 61850 standard, evaluated through hardware-in-the-loop (HIL) testing. The study utilized an already existing model of a modern power grid consisting of the IEEE 9-bus power system integrated with a large-scale wind power plant (LSWPP). The SEL-487B Relay was configured to protect the PCC using a low-impedance busbar differential monitoring and protection system equipped with adaptive setting group logic that automatically transitions between Group 1 and Group 2 based on system loading conditions. Significant steps were followed for selecting and configuring instrument transformers and implementing relay logic in compliance with IEEE and IEC standards. Real-time digital simulation using Real-Time Digital Simulator (RTDS) hardware and its software, Real-time Simulation Computer-Aided Design (RSCAD), was used to assess the performance of the overall monitoring and protection system, focusing on the monitoring and publishing of the selected electrical and mechanical measurements from a selected wind turbine generator unit (WTGU) on the LSWPP side through the IEC 61850 standard network, and on the behavior of the monitoring and protection system under initial and increased load conditions through monitoring of differential and restraint currents. The overall monitoring and protection system was tested under both initial and increased load conditions, confirming its capability to reliably publish analog values from WTGU13 for availability on the IEC 61850 standard network while maintaining secure protection operation. Quantitatively, the measured differential (operate) and restraint currents were 0.32 PU and 4.38 PU under initial loading, and 1.96 PU and 6.20 PU under increased loading, while total fault clearance times were 606.667 ms and 706.667 ms for faults under initial load and increased load demand conditions, respectively. These results confirm that the developed framework provides accurate real-time monitoring and reliable operation for faults, while demonstrating a practical and replicable solution for monitoring and protection at transmission-level PCCs within renewable-integrated networks. Full article

Background: Tyrosinase is a copper-dependent oxyreductase capable of catalyzing the oxidation of mono- and diphenols. Its activity is crucial in the biosynthetic pathway of melanin, the pigment responsible for the pigmentation of mammalian skin and fur, and protecting their skin from harmful UV radiation. Overproduction of this pigment leads to numerous pathological conditions, including the most severe form of skin cancer—malignant melanoma. Furthermore, tyrosinase produced in plant tissues leads to the browning of damaged vegetables and fruits. Therefore, the search for compounds that effectively and efficiently control tyrosinase activity is desirable for both pharmaceutical and food applications. Methods: A group of six boronate derivatives of thiosemicarbazones was synthesized, and their inhibitory properties against tyrosinase were determined. Furthermore, their ability to inhibit melanogenesis and proliferation in SK-MEL-3 and Hs294T cells was investigated. Docking simulations were performed to determine the nature of the inhibitor–protein interactions. Results: The tested inhibitors exhibited half-maximal inhibitory concentrations (IC₅₀) in the micromolar range. The best inhibitor, compound 6, had an IC₅₀ of 1.4 µM. The tested compounds exhibited poor selectivity for cell lines capable of high and low tyrosinase overexpression, with inhibitor 4 proving to be the most selective compound among those tested. Molecular modeling results indicate that the compounds with the highest activity against tyrosinase interact with the active cavity and the copper ions present within it via a boron moiety substituted on the aromatic ring of the thiosemicarbazones. Cell-based experiments indicated limited antiproliferative effects up to 100 µM across the tested lines. The compounds demonstrated weak antiproliferative effects in SK-MEL-3 and Hs-294T up to 100 µM. Conclusions: Our results show that the introduction of a boronic acid moiety is an alternative to carboxylic acid derivatives, improving the inhibitory activity of boron analogs (by fourfold) against fungal tyrosinase. Full article

Radon exposure is the second leading cause of lung cancer worldwide, yet monitoring strategies remain limited, expensive, and unevenly applied. Recent advances in the Internet of Things (IoT) offer the potential to change radon surveillance through low-cost, real-time, distributed sensing networks. This review consolidates emerging research on IoT-based radon monitoring, drawing from both primary radon studies and analogous applications in environmental IoT. A search across six major databases and relevant grey literature yielded only five radon-specific IoT studies, underscoring how new this research field is rather than reflecting a shortcoming of the review. To enhance the analysis, we delve into sensor physics, embedded system design, wireless protocols, and calibration techniques, incorporating lessons from established IoT sectors like indoor air quality, industrial safety, and volcanic gas monitoring. This interdisciplinary approach reveals that many technical and logistical challenges, such as calibration drift, power autonomy, connectivity, and scalability, have been addressed in related fields and can be adapted for radon monitoring. By uniting pioneering efforts within the broader context of IoT-enabled environmental sensing, this review provides a reference point and a future roadmap. It outlines key research priorities, including large-scale validation, standardized calibration methods, AI-driven analytics integration, and equitable deployment strategies. Although radon-focused IoT research is still at an early stage, current progress suggests it could make continuous exposure assessment more reliable, affordable, and widely accessible with clear public health benefits. Full article

Marine Bacteroidota are recognized bacterial producers of bioactive metabolites, yet their biosynthetic potential remains cryptic under standard laboratory conditions. Here, we developed chemically defined media for Fulvivirga kasyanovii 48LL (Cytophagia) and Tenacibaculum mesophilum fLL (Flavobacteriia) to evaluate the effect of environmentally relevant carbon sources on growth and secondary metabolism. F. kasyanovii utilized 31 of 34 tested carbon sources whereas T. mesophilum grew on only five substrates, underscoring a distinct nutritional preferences. Substrate significantly influenced the antibacterial activity of F. kasyanovii extracts. Growth on β-1,3-glucan, glycerol, poly(β-hydroxybutyrate) (PHB), fish gelatin, or pectin resulted in extracts generating the largest inhibition zones (10–13 mm) against Bacillus subtilis or Rossellomorea marisflavi. Genome analysis revealed F. kasyanovii to be enriched in biosynthetic gene clusters (BGCs), notably harboring a ~570 kb genomic island comprising five large NRPS/PKS-type clusters. Quantitative PCR confirmed carbon-source-dependent regulation of these operons: glucose induced BGC1, BGC3, and BGC4, while κ-carrageenan and PHB upregulated BGC2. Conversely, yeast–peptone medium (analogous to standard marine broth) repressed transcription across all active clusters. These findings demonstrate that naturally occurring carbon sources can selectively activate cryptic BGCs and modulate antibacterial activity in F. kasyanovii, suggesting that similar strategy can be used for natural-product discovery in marine Bacteroidota. Full article

The systematic economic development of European Union member states has resulted in a dynamic increase in road transport, accompanied by adverse environmental impacts. Consequently, research efforts have focused on identifying technical solutions to reduce fuel and/or energy consumption. One promising approach involves the formation of homogeneous and heterogeneous vehicle platoons. This study presents the results of numerical simulations and analyses of aerodynamic and aeroacoustic phenomena generated by heterogeneous vehicle platoons composed of passenger cars, delivery vans, and trucks. A total of 54 numerical models were developed in various configurations, considering three vehicle speeds and three inter-vehicle distances. The analysis was conducted using Computational Fluid Dynamics (CFD) methods with the following two turbulence models: the k–ω Shear Stress Transport (SST) model and Large Eddy Simulation (LES), combined with the Ffowcs Williams–Hawkings acoustic analogy to determine sound pressure levels. Verification calculations were performed using methods dedicated to environmental noise analysis, supplemented by acoustic field measurements. The results conclusively demonstrate that vehicle movement in specific platoon configurations can lead to significant fuel and/or energy savings, as well as reductions in harmful emissions. This solution may be implemented in the future as an integral component of Intelligent Transportation Systems (ITSs) and Intelligent Environmental Management Systems (IEMSs). Full article

Dynamic pressure measurement is an important component in the turbo engine testing process. This paper presents a comparative analysis between two types of multichannel electronic pressure measurement systems, commonly known as pressure scanners, used for this purpose: ZOC17/8Px, with analog amplification per channel, and MPS4264, a modern digital system with integrated A/D conversion. The study was conducted in two stages: a metrological verification and validation in static mode, using a high-precision pressure standard, and an experimental stage in dynamic mode, where data was acquired from a turbojet engine test stand, in constant engine speed mode. The signal stability of the pressure scanners was statistically analyzed by determining the coefficient of variation in the signal and the frequency spectrum (FFT) for each channel of the pressure scanners. Furthermore, comprehensive uncertainty budgets were calculated for both systems. The results highlight the superior stability and reduced uncertainty of the MPS4264 pressure scanner, attributing its enhanced performance to digital integration and a higher resilience to external noise. The findings support the adoption of modern digital systems for dynamic applications and provide a robust metrological basis for the optimal selection of measurement systems. Full article

Fertility-sparing treatments (FSTs) have gained importance for young female cancer patients, especially those with early-stage cervical, ovarian, and endometrial cancers. However, concerns about the long-term safety of these procedures, particularly in more advanced cancers, persist. A literature review was conducted using databases such as PubMed, Scopus, and Web of Science. The search terms included “fertility preservation” and “gynaecological cancer”. Articles published between 2014 and 2024 were considered, with 39 articles cited in the paper. The inclusion criteria were female patients undergoing FST. Studies were excluded if prior treatments impacted fertility or if oncological outcomes were inadequately reported. Radical trachelectomy, laparoscopic fertility-sparing surgeries, and cryopreservation techniques, such as ovarian tissue vitrification and oocyte cryopreservation, offer viable options for preserving fertility in early-stage gynecological cancer patients. Radical trachelectomy and cryopreservation showed positive reproductive outcomes, with pregnancy rates of 30–50% in early-stage cases. GnRH analogs during chemotherapy also demonstrated benefits in maintaining fertility. Despite these advances, recurrence in more advanced stages (FIGO IA2 and beyond) remains a concern. Minimally invasive surgeries like robotic-assisted procedures demonstrated comparable fertility outcomes to traditional methods but with fewer complications. FST is a promising option for women with early-stage cancer, offering favorable reproductive and survival outcomes. However, further research is needed to confirm long-term oncological safety in advanced stages. Multidisciplinary approaches and individualized treatment planning are essential for optimizing outcomes. Full article

(1) Background: Low back pain (LBP) is the most prevalent cause of disability worldwide, yet current assessment relies mainly on subjective questionnaires, underscoring the need for objective and interpretable biomarkers. Bioelectrical impedance parameter (BIP), quantified by resistance (R), impedance magnitude (Z), and phase angle (PA), reflects tissue hydration and cellular integrity and may provide physiological correlates of pain; (2) Methods: This cross-sectional study used lumbar BIP and demographic characteristics from 83 participants (38 with lumbar BIP and 45 normal controls). We applied Extreme Gradient Boosting (XGBoost), a regularized tree-based machine learning (ML) algorithm, with stratified five-fold cross-validation. Model interpretability was ensured using SHapley Additive exPlanations (SHAP), which provide global importance rankings and local feature attributions. Outcomes included classification of LBP versus healthy status and regression-based prediction of pain scales: the Visual Analog Scale (VAS), Oswestry Disability Index (ODI), and Roland–Morris Disability Questionnaire (RMDQ); (3) Results: The classifier achieved high discrimination (ROC–AUC = 0.996 ± 0.009, sensitivity = 0.950 ± 0.068, specificity = 0.977 ± 0.049). Pain prediction showed best performance for VAS (R² = 0.70 ± 0.14; mean absolute error = 1.23 ± 0.27), with weaker performance for ODI and RMDQ; (4) Conclusions: These findings suggest that explainable ML models applied to BIP could discriminate between LBP and healthy groups and could estimate pain intensity, providing an objective complement to subjective assessments. Full article

Background and Objectives: Spinal anesthesia is increasingly preferred in pediatric surgeries due to its rapid onset, high success rate, and low risk of systemic complications. However, the influence of age and body mass index (BMI) on block characteristics in adolescents remains insufficiently studied. Materials and Methods: This retrospective study evaluated 190 pediatric patients (aged 9–18 years; 154 male, 36 female) undergoing elective surgery with spinal anesthesia. Patients were stratified by age (Group 1: 9–14 years; Group 2: 15–18 years) and BMI (Group A: 16.00–19.65 kg/m²; Group B: 19.66–23.31 kg/m²). The primary outcome was sensory block duration. Secondary outcomes included sedation requirements, complications, analgesic requirement times, and Visual Analog Scale (VAS) scores. Results: Group 2 had significantly longer sensory block duration and lower postoperative VAS scores at 3 and 12 h compared to Group 1 (p < 0.001). Lower BMI was associated with greater sedation requirements (p < 0.001) and a higher incidence of intraoperative nausea and vomiting (p = 0.013). Complications were infrequent (hypotension 6.3%, bradycardia 2.1%, PONV 7.1%, postoperative headache 3.1%) and managed conservatively. Conclusions: Age and BMI meaningfully influence spinal anesthesia characteristics in pediatric patients. Older adolescents achieved longer sensory block durations and better postoperative analgesia, whereas younger and lower-BMI patients required more sedation and had higher nausea rates. Individualized spinal anesthesia planning, considering age, BMI, and developmental stage, may optimize clinical outcomes. Prospective studies are warranted to validate these findings. Full article