Search Results (194)

Antimicrobial resistance (AMR) has emerged as a planetary health emergency, driven not only by the clinical misuse of antibiotics but also by diverse environmental dissemination pathways. This review critically examines the role of environmental compartments—water, soil, and air—as dynamic reservoirs and transmission routes for antibiotic-resistant bacteria (ARB) and resistance genes (ARGs). Recent metagenomic, epidemiological, and mechanistic evidence demonstrates that anthropogenic pressures—including pharmaceutical effluents, agricultural runoff, untreated sewage, and airborne emissions—amplify resistance evolution and interspecies gene transfer via horizontal gene transfer mechanisms, biofilms, and mobile genetic elements. Importantly, it is not only highly polluted rivers such as the Ganges that contribute to the spread of AMR; even low concentrations of antibiotics and their metabolites, formed during or after treatment, can significantly promote the selection and dissemination of resistance. Environmental hotspots such as European agricultural soils and airborne particulate zones near wastewater treatment plants further illustrate the complexity and global scope of pollution-driven AMR. The synergistic roles of co-selective agents, including heavy metals, disinfectants, and microplastics, are highlighted for their impact in exacerbating resistance gene propagation across ecological and geographical boundaries. The efficacy and limitations of current mitigation strategies, including advanced wastewater treatments, thermophilic composting, biosensor-based surveillance, and emerging regulatory frameworks, are evaluated. By integrating a One Health perspective, this review underscores the imperative of including environmental considerations in global AMR containment policies and proposes a multidisciplinary roadmap to mitigate resistance spread across interconnected human, animal, and environmental domains. Full article

Air purification is a key process aimed at removing harmful impurities and providing a safe and comfortable environment for human life and work. This study presents the results of an investigation into the composition, textural, and sorption properties of a multichannel carbon filtering material developed for air purification from biological (infectious) contaminants. The filtering block has a cylindrical shape and is manufactured by extrusion of a plastic composition based on carbonized rice husk with the addition of binding agents, followed by staged thermal treatment (calcination, activation, and demineralization). The filter’s effectiveness is based on the inactivation of pathogenic microorganisms as the air passes through the porous surface of the sorbent, which is modified with broad-spectrum antiseptic agents (active against bacteria, bacilli, fungi, and protozoa). X-ray diffraction analysis revealed the presence of amorphous carbon in a tubostratic structure, with a predominance of sp- and sp²-hybridized carbon atoms not incorporated into regular graphene lattices. IR spectroscopy demonstrated the presence of reactive functional groups characteristic of the developed porous structure of the material, which is capable of selective sorption of antiseptic molecules. SEM surface analysis revealed an amorphous texture with a loose structure and elements in the form of spherical semi-ring formations formed by overlapping carbon plates. An experimental setup was also developed using cylindrical multichannel carbon blocks with a diameter of 48 mm, a length of 120 mm, and 100–120 longitudinal channels with a cross-section of 1 mm². The obtained results confirm the potential of the proposed material for use in air purification and disinfection systems under conditions of elevated biological risk. Full article

Bioaerosol particles contribute to the reduced indoor air quality and cause various health issues, thus their concentration must be managed. Air cleaning is one of the most viable technological options for reducing quantities of indoor air contaminants. This study assesses the effectiveness of a prototype multi-stage air cleaner in reducing bioaerosol particle viability and concentrations. The single-pass type unit consisted of non-thermal plasma (NTP), ultraviolet-C (UV-C) irradiation, bipolar ionization (BI), and electrostatic precipitation (ESP) stages. The device was tested under controlled laboratory conditions using Escherichia coli (Gram-negative) and Lactobacillus casei (Gram-positive) bacteria aerosol at varying airflow rates (50–600 m³/h). The device achieved over 99% inactivation efficiency for both bacterial strains at the lowest airflow rate (50 m³/h). Efficiency declined with increasing airflow rates but remained above 94% at the highest flow rate (600 m³/h). Among the individual stages, NTP demonstrated the highest standalone inactivation efficiency, followed by UV-C and BI. The ESP stage effectively captured inactivated bioaerosol particles, preventing re-emission, while an integrated ozone decomposition unit maintained ozone concentrations below safety thresholds. These findings show the potential of multi-stage air cleaning technology for reducing bioaerosol contamination in indoor environments, with applications in healthcare, public spaces, and residential settings. Full article

Objective: Current good manufacturing practice (cGMP) guidance for positron emission tomography (PET) drugs has been established in Europe and the United States. In Japan, the Pharmaceuticals and Medical Devices Agency (PMDA) approved the use of radiosynthesizers as medical devices for the in-house manufacturing of PET drugs in hospitals and clinics, regardless of the cGMP environment. Without adequate facilities, equipment, and personnel required by cGMP regulations, the quality assurance (QA) and clinical effectiveness of PET drugs largely depend on the radiosynthesizers themselves. To bridge the gap between radiochemistry standardization and site qualification, the Japanese Society of Nuclear Medicine (JSNM) has issued guidance for the in-house manufacturing of small-scale PET drugs under academic GMP (a-GMP) environments. The goals of cGMP and a-GMP are different: cGMP focuses on process optimization, certification, and commercialization, while a-GMP facilitates the small-scale, in-house production of PET drugs for clinical trials and patient-specific standard of care. Among PET isotopes, N-13 has a short half-life (10 min) and must be synthesized on site. [¹³N]Ammonia ([¹³N]NH₃) is used for myocardial perfusion imaging under the Japan Health Insurance System (JHIS) and was thus selected as a working example for the manufacturing of PET drugs in an a-GMP environment. Methods: A [¹³N]NH₃-radiosynthesizer was installed in a hot cell within an a-GMP-compliant radiopharmacy unit. To comply with a-GMP regulations, the air flow was adjusted through HEPA filters. All cabinets and cells were disinfected to ensure sterility once a month. Standard operating procedures (SOPs) were applied, including analytical methods. Batch records, QA data, and radiation exposure to staff in the synthesis of [¹³N]NH₃ were measured and documented. Results: 2.52 GBq of [¹³N]NH₃ end-of-synthesis (EOS) was obtained in an average of 13.5 min in 15 production runs. The radiochemical purity was more than 99%. Exposure doses were 11 µSv for one production run and 22 µSv for two production runs. The pre-irradiation background dose rate was 0.12 µSv/h. After irradiation, the exposed dosage in the front of the hot cell was 0.15 µSv/h. The leakage dosage measured at the bench was 0.16 µSv/h. The exposure and leakage dosages in the manufacturing of [¹³N]NH₃ were similar to the background level as measured by radiation monitoring systems in an a-GMP environments. All QAs, environmental data, bacteria assays, and particulates met a-GMP compliance standards. Conclusions: In-house a-GMP environments require dedicated radiosynthesizers, documentation for batch records, validation schedules, radiation protection monitoring, air and particulate systems, and accountable personnel. In this study, the in-house manufacturing of [¹³N]NH₃ under a-GMP conditions was successfully demonstrated. These findings support the international harmonization of small-scale PET drug manufacturing in hospitals and clinics for future multi-center clinical trials and the development of a standard of care. Full article

The COVID-19 pandemic highlighted the urgent need for sustainable and scalable air disinfection technologies in HVAC systems, addressing the limitations of energy-intensive and chemically intensive conventional methods. This study developed and evaluated a pilot experimental installation integrating plasma chemistry and photocatalysis for airborne pathogen and pollutant mitigation. The installation, designed with a modular architecture to simulate real-world HVAC dynamics, employed a bipolar plasma ioniser, a TiO₂ photocatalytic module, and an adsorption-catalytic module for ozone abatement. Characterization techniques, including SEM and BET analysis, were used to evaluate the morphology and surface properties of the catalytic materials. Field tests in a production room demonstrated a 60% reduction in airborne microflora in three days, along with effective decomposition of ozone. The research also determined the optimal electrode geometry and interelectrode distance for stable corona discharge, which is essential for efficient plasma generation. Energy-efficient design considerations, which incorporate heat recovery and heat pump integration, achieved a 7–8-fold reduction in air heating energy consumption. These results demonstrate the potential of integrated plasma photocatalysis as a sustainable and scalable approach to enhance indoor air quality in centralised HVAC systems, contributing to both public health and energy efficiency. Full article

(1) Background: Improper disinfection of slurry and municipal wastewater poses a serious threat to public health. These fluids are reservoirs of viruses, bacteria, fungi and parasites. (2) Methods: This study aimed to evaluate, on a laboratory scale, the disinfection effectiveness of fine bubble aeration with air activated by radiant catalytic ionization (RCI) against Enterococcus faecalis, Escherichia coli, Salmonella Senftenberg W775, Listeria monocytogenes, Clostridioides difficile, Aspergillus niger and Ascaris suum eggs in comparison to conventional atmospheric air aeration. The inactivation kinetics was calculated on the basis of Weibull and first-order models. (3) Results: The final number of microorganisms on the last day in the slurry disinfected with RCI ranged from 1.14 × 10² for L. monocytogenes to 1.91 × 10⁷ CFU (colony-forming unit) × mL⁻¹ for C. difficile. After using atmospheric air aeration, the bacteria number ranged from 2.82 × 10³ for L. monocytogenes to 2.24 × 10⁷ CFU × mL⁻¹ for C. difficile. In the case of aeration using RCI technology, the maximum time required to eliminate 99.9% of the microorganisms population was 20.84 days in slurry and 16.40 days in wastewater and was determined for A. niger. In the case of atmospheric air, this time was 47.76 days in slurry and 28.74 days in wastewater and was determined for C. difficile. In turn, the time to inactivate the number of invasive A. suum eggs by 90% was 20.70 and 24.61 weeks for RCI and 21.33 and 27.82 weeks for atmospheric air, respectively. Both in the case of slurry and municipal wastewater, disinfection with RCI was more effective than aeration with atmospheric air. (4) Conclusions: Our study, for the first time, exploits the possibility of using RCI in aeration to improve the efficiency of pathogen elimination from wastewater and slurry. Full article

Airborne transmission has been implicated as a major route for the spread of microorganisms, causing infectious disease outbreaks worldwide. This has been emphasized by the recent COVID-19 pandemic, caused by the SARS-CoV-2 virus. There is thus an unmet need to develop technologies that arrest the spread of airborne infectious diseases by inactivating viruses in the air. In this study, the efficacy of two commercially available air ionizer systems for inactivating the bacteriophage MS2, which has been utilized as a surrogate of SARS-CoV-2 as well as a surrogate of noroviruses, was assessed. An experimental test apparatus similar to an HVAC duct system was utilized for the efficacy testing. Each of the two ionizer devices was challenged with viral aerosols of the bacteriophage MS2. The results indicate that the two ionizers were able to reduce the concentration of bacteriophage MS2 virus in the air by 82.02% and 81.72%, respectively. These results point to the efficacy of these ionizer devices in inactivating airborne microorganisms and thus making them an important tool in arresting the spread of infectious diseases. More studies are needed to assess their efficacy against other important airborne viruses such as influenza and strains of the SARS-CoV-2 virus. Full article

As the epidemic affected everyone across the world, the solution to the epidemic was developed globally. Many applications adopt Internet of Things (IoT) technology to detect epidemics, and effective monitoring systems are developed to monitor air pollution, personal transmission, early detection of serious cases, and remote assessment. However, care facilities in an aging society require effective disinfection and sterilization to prevent viral transmission. We integrated the interactive and real-time features of the Internet of Things (IoT) to design and build an intelligent self-propelled sterilization robot for sterilization. Intelligent sterilization and disinfection planning and task allocation mechanisms were designed for sterilization in clinics. For healthcare facilities, the developed robot can reduce the burden on healthcare professionals, help to manage the disinfection and sterilization process, and ensure patient safety. At the same time, robots promote the development of epidemic prevention industries and prepares for future attacks from harmful air pollutants or new infections. Full article

The global COVID-19 pandemic has highlighted the pivotal role of microbial disinfection technologies, driving the demand for innovative, efficient, and sustainable solutions. Triboelectric technology, known for efficiently converting ambient mechanical energy into electrical energy, has emerged as a promising candidate to address these needs. Self-powered electro-based microbial disinfection using triboelectric nanogenerators (TENGs) has emerged as a promising solution. TENGs have demonstrated effective disinfection capabilities in various settings, including water, air, surfaces, and wounds. This review explores the advancements in TENG-based microbial disinfection, highlighting its mechanisms and applications. By utilizing triboelectric technology, it provides comprehensive insights into the development of sustainable and efficient solutions for microbial control across diverse environments. Full article

Zinc oxide (ZnO) photocatalysts have emerged as a promising material for environmental and energy applications due to their exceptional photocatalytic properties. Initially recognized for their efficiency under ultraviolet (UV) light, recent advancements have focused on enhancing ZnO’s visible light activity (VLA) to address its inherent limitations. This review provides an overview of ZnO’s structure, electronic properties, and photocatalytic mechanisms. Various strategies for modifying ZnO to harness visible light, including metal and non-metal doping, dye sensitization, and semiconductor coupling, are discussed. Special emphasis is placed on the mechanisms behind visible light absorption and reactive oxygen species (ROS) generation, as deduced through physicochemical and photoelectrochemical analyses. The applications of ZnO in environmental remediation are comprehensively explored, particularly for water treatment, disinfection, and air purification. The photocatalytic degradation of pollutants, including persistent organic compounds, pharmaceuticals, dyes, and pesticides, using ZnO is reviewed and compared with conventional UV-activated ZnO materials. This review underscores the potential of ZnO as an efficient and sustainable solution for environmental purification. Full article

In this study, the authors carried out a multiparametric assessment of the influence of swirl patterns during aerosol flow on the shape of the interfacial area that forms the cone based on data obtained from experimental measurements using the PIV and LLS methods. The results were correlated with the disinfection process occurring in the near and far fields of the aerosol (direct surface disinfection and volume fogging). In this study, parameters such as turbulent kinetic energy (TKE), swirl strength (SS), pressure fields, and Sauter mean diameter (d₃₂) are used to investigate the relationship between aerosol spray morphology and flow dynamics under different operating conditions. Three different geometrical settings of the aerosol-generating system and two different pressures corresponding to the air supply to the spray nozzle have been adopted. By evaluating the results obtained, the influence of each parameter on the formation of the aerosol displacement trajectory, the stabilization of the spray cone, and its degradation was identified. The shape of the boundary between the dynamically moving aerosol and the surrounding air was also evaluated. The conditions for swirling and straight-line flows within the aerosol cone, and, thus, the conditions for the volumetric development of swirling phenomena, were further clarified. Full article

Among nanomaterials, silver nanoparticles (AgNPs) are cost-effective and exhibit unique physicochemical properties that enable them to become the most used agents for the manufacture of various products known as nano-enabled, including those for personal care, drugs, fabrics, sprays, disinfectants, vacuum cleaners, and air conditioners, with a continuous expansion to different sectors. Industrial discharges, the disposal of wastewater treatment effluents, and indirect runoff from the soil are some factors that are increasing the accumulation of AgNPs in aquatic and wetland ecosystems. Herewith, we critically analyze the progress in the research of the uptake and translocation of AgNPs in aquatic and wetland plants and their phytotoxic effect that depends on the concentration, size, distribution, morphological shape, surface characteristics and chemical composition of the nanoparticles, as well as the plant genotypes, among other factors. Due to biological plasticity, the toxicity level of AgNPs may vary among plant species, which may be further affected by the mode of application, time of exposure, and plant conditions (e.g., agronomic management, growth rate, phenological stage, etc.). Therefore, it is possible to identify and select competent plants for phytoremediation purposes, including superior capabilities for phytoextraction, phytofiltration, and phytostabilization. The review also identifies the main gaps that require attention in future research in order to elucidate a more integrative map aimed to reduce the potential threats to the environment and living organisms including humans. Full article

Chemical pollution is an increasing worldwide concern, with children being especially vulnerable to the harmful effects of air pollution. This study aimed to characterize the mixture of volatile organic compounds (VOCs) present in indoor air across residential, educational, and recreational settings. It analyzed data on VOC concentrations from previous sampling campaigns conducted in households with children, primary schools, and indoor swimming pools (70 buildings, 151 indoor spaces) in northern Portugal. The findings reveal the co-occurrence of 16 VOCs (1,2,4-trimethylbenzene, benzene, ethylbenzene, m/o/p-xylenes, styrene, toluene, tetrachloroethylene, 2-ethylhexanol, butanol, acetophenone, ethyl acetate, benzaldehyde, decanal, nonanal, 1-methoxy-2-propanol and limonene) across all three settings, primarily associated to emissions from building materials and detergents. However, distinct patterns were also observed in the VOCs detected across the three indoor environments: in homes, the predominant VOCs were primarily released from cleaning and fragranced products; in schools, from ammonia-based cleaners and occupant activities; and in swimming pools, the predominant airborne chemicals were disinfection by-products resulting from the chemical dynamics associated with water disinfection. Overall, the findings highlight the need for additional research to deepen our understanding of the risks posed by combined exposure to multiple indoor air chemicals for children. These results also underscore the importance of developing and enforcing regulations to monitor VOC levels in environments frequented by children and implementing preventive measures to minimize their exposure to harmful chemicals. Full article

Background/Objectives: In healthcare environments with high microbial loads, effective infection control measures are critical for reducing airborne and surface contamination. One of the novel modalities in the achievement of these goals is the use of antimicrobial mists, such as droplets, in the form of dry fog. Although the usage of dry fog in the disinfection of contained healthcare microenvironments is well known, the effect of such a system in terms of a meaningful reduction in the microbial burden in an open inpatient ward is unclear. Our objective was to assess the impact of scheduled dry fogging on microbial reduction in such settings. Methods: We collected air and surface samples from rooms receiving daily, biweekly, or no fogging (controls) over six months, establishing the baseline contamination and evaluating the reduction trends in treated rooms. The “reduction effect” was measured by tracking microbial isolation trends before and after treatment, while the “degree of reduction” assessed differences across rooms with varied disinfection schedules. Results: The results indicate that scheduled dry fogging significantly reduced microbial loads in treated rooms, especially with daily disinfection (SE = 64.484, p = 0.002). The airborne contamination in treated rooms showed a strong downward trend over time (SE = 19.192, p < 0.001). Surface contamination remained challenging due to frequent recontamination; however, treated rooms exhibited a consistent reduction in microbial presence (SE = 2.002, p = 0.010), confirming dry fogging’s role as a valuable adjunct to routine cleaning. Conclusions: In conclusion, this study highlights that dry fogging effectively reduces microbial loads in open, high-traffic healthcare environments, supporting its use as part of a multimodal infection control strategy. Full article

This study investigates a series of surface-active ionic liquids (SAILs), including both imidazolium monocationic and dicationic compounds. These compounds are promising candidates, as they combine unique surface properties with antimicrobial activity, aligning with modern trends in chemistry. The research encompasses synthesis, thermal analysis, and topographical assessment, focusing on the impact of the amphiphilic cationic moiety, alkyl chain length, and the spatial relationship between the imidazolium ring and the phenyl substituent on the compounds’ physicochemical behavior. An added value of this work lies in the integration of theoretical calculations related to their behavior in solution and at the air–water interface, revealing spontaneous adsorption (negative Gibbs free energy of adsorption values, ΔG⁰_ads). The results indicate that dicationic imidazolium SAILs have a greater tendency to form micelles but are less effective at reducing surface tension compared to their monocationic counterparts. Topography analyses of SAILs with 12 carbon atoms further highlight these differences. Notably, the dicationic SAIL with 12 carbon atoms in the spacer exhibited an impressive MIC of 0.007 mmol L⁻¹ against Candida albicans, consistent with findings showing that dicationic SAILs outperformed conventional antifungal agents, such as amphotericin B and fluconazole, at equivalent concentrations. Overall, the synthesized SAILs demonstrate superior surface activity compared to commercial surfactants and show potential as disinfectant agents. Full article