Search Results (3,423)

Soil salinization constitutes a major constraint on global agricultural production, with marked divergence in salt adaptation strategies between salt-tolerant barley (Hordeum vulgare) and salt-sensitive rice (Oryza sativa). This study systematically investigated the evolution and functional specialization of the C3HC4-type RING zinc finger gene family, known to mediate abiotic stress responses through E3 ubiquitin ligase activity, in these contrasting cereal species. Through comparative genomics, we identified 123 HvC3HC4 genes and 90 OsC3HC4 genes, phylogenetically classified into four conserved subgroups. Differences in C3HC4 genes in phylogenetic relationships, chromosomal distribution, gene structure, motif composition, gene duplication events, and cis-elements in the promoter region were observed between barley and rice. Moreover, HvC3HC4s in barley tissues preferentially adopted an energy-conserving strategy, which may be a key mechanism for barley’s higher salt tolerance. Additionally, we found that C3HC4 genes were evolutionarily conserved in salt-tolerant species. The current results reveal striking differences in salt tolerance between barley and rice mediated by the C3HC4 gene family and offer valuable insight for potential genetic engineering applications in improving crop resilience to salinity stress. Full article

Hydrogels, characterized by their high water content, tunable mechanical properties, and excellent biocompatibility, have emerged as a promising material platform for the preservation of cultural heritage. Their unique physicochemical features enable non-invasive and adaptable solutions for environmental regulation, structural stabilization, and antifungal protection. This review provides a comprehensive overview of recent progress in hydrogel-based strategies specifically developed for the conservation of cultural relics, with a particular focus on antifungal performance—an essential factor in preventing biodeterioration. Current hydrogel systems, composed of natural or synthetic polymer networks integrated with antifungal agents, demonstrate the ability to suppress fungal growth, regulate humidity, alleviate mechanical stress, and ensure minimal damage to artifacts during application. This review also highlights future research directions, such as the application prospects of novel materials, including stimuli-responsive hydrogels and self-dissolving hydrogels. As an early exploration of the use of hydrogels in antifungal protection and broader cultural heritage conservation, this work is expected to promote the wider application of this emerging technology, contributing to the effective preservation and long-term transmission of cultural heritage worldwide. Full article

The Santurbán paramo is a sensitive high-mountain ecosystem exposed to pressures from extractive and agricultural activities, as well as increasing tourism. In response, this study presents a context-aware recommendation system designed to support sustainable tourism through the integration of deep neural networks and ontology-based semantic modeling. The proposed system delivers personalized recommendations—such as activities, accommodations, and ecological routes—by processing user preferences, geolocation data, and contextual features, including cost and popularity. The architecture combines a trained TensorFlow Lite model with a domain ontology enriched with GeoSPARQL for geospatial reasoning. All inference operations are conducted locally on Android devices, supported by SQLite for offline data storage, which ensures functionality in connectivity-restricted environments and preserves user privacy. Additionally, the system employs geofencing to trigger real-time environmental notifications when users approach ecologically sensitive zones, promoting responsible behavior and biodiversity awareness. By incorporating structured semantic knowledge with adaptive machine learning, the system enables low-latency, personalized, and conservation-oriented recommendations. This approach contributes to the sustainable management of natural reserves by aligning individual tourism experiences with ecological protection objectives, particularly in remote areas like the Santurbán paramo. Full article

The genus Sonchus (Asteraceae) comprises 98 species, including 17 predominantly herbaceous taxa native to the Mediterranean region. These plants have long been utilized as traditional wild food sources due to their high nutritional value, as they are rich in vitamins A, C, and K, essential minerals, and bioactive compounds with antioxidant and anti-inflammatory properties. This review aims to provide a comprehensive synthesis of the taxonomy, geographic distribution, phytochemical composition, traditional uses, historical significance, and pharmacological properties of Sonchus species. A systematic literature search was conducted using PubMed, Scopus, Web of Science, and Google Scholar, focusing on studies from 1980 to 2024. Inclusion and exclusion criteria were applied, and methodological quality was assessed using standardized tools. A bibliometric analysis of 440 publications (from 1856 to 2025) reveals evolving research trends, with S. oleraceus, S. arvensis, and S. asper being the most extensively studied species. The review provides detailed taxonomic insights into 17 species and 14 subspecies, emphasizing their ecological adaptations and biogeographical patterns. Additionally, it highlights the cultural and medicinal relevance of Sonchus since antiquity while underscoring the threats posed by environmental degradation and changing dietary habits. Sonchus oleraceus and S. tenerrimus dominate the culinary applications of the genus, likely due to favorable taste, wide accessibility, and longstanding cultural importance. The comprehensive nutritional profile of Sonchus species positions these plants as valuable contributors to dietary diversity and food security. Finally, the study identifies current knowledge gaps and proposes future research directions to support the conservation and sustainable utilization of Sonchus species. Full article

The Qinghai-Tibetan Plateau is one of the world’s most prominent biodiversity hotspots. Understanding the spatial patterns of ecological uniqueness in its plant communities is essential for uncovering the mechanisms of community assembly and informing effective conservation strategies. In this study, we analyzed data from 758 plots across 338 sites on the Qinghai-Tibetan Plateau. For each plot, the vegetation type was classified, and all plant species present, along with their respective abundance or coverage, were recorded in the database. To assess overall compositional variation, community β-diversity was quantified, while a plot-level approach was applied to determine the influence of local environmental conditions and community characteristics on ecological uniqueness. We used stepwise multiple regressions, variation partitioning, and structural equation modeling to identify the key drivers of spatial variation in ecological uniqueness. Our results show that (1) local contributions to β-diversity (LCBD) exhibit significant geographic variation—increasing with longitude, decreasing with latitude, and showing a unimodal trend along the elevational gradient; (2) shrubs and trees contribute more to β-diversity than herbaceous species, and LCBD is strongly linked to the proportion of rare species; and (3) community characteristics, including species richness and vegetation coverage, are the main direct drivers of ecological uniqueness, explaining 36.9% of the variance, whereas climate and soil properties exert indirect effects through their interactions. Structural equation modeling further reveals a coordinated influence of soil, climate, and community attributes on LCBD, primarily mediated through soil nutrient availability. These findings provide a theoretical basis for adaptive biodiversity management on the Qinghai-Tibetan Plateau and underscore the conservation value of regions with high ecological uniqueness. Full article

This paper addresses the trajectory tracking control problem of underactuated ships in ocean engineering, which faces the dual challenges of tracking error time–performance regulation and robustness design due to the system’s underactuated characteristics, model uncertainties, and external disturbances. Aiming to address the issues of traditional finite-time control (convergence time dependent on initial states) and fixed-time control (control chattering and parameter conservativeness), this paper proposes a predefined-time adaptive control framework that integrates an event-triggered mechanism and neural networks. By constructing a Lyapunov function with time-varying weights and designing non-periodic dynamically updated dual triggering conditions, the convergence process of tracking errors is strictly constrained within a user-prespecified time window without relying on initial states or introducing non-smooth terms. An adaptive approximator based on radial basis function neural networks (RBF-NNs) is employed to compensate for unknown nonlinear dynamics and external disturbances in real-time. Combined with the event-triggered mechanism, it dynamically adjusts the update instances of control inputs, ensuring prespecified tracking accuracy while significantly reducing computational resource consumption. Theoretical analysis shows that all signals in the closed-loop system are uniformly ultimately bounded, tracking errors converge to a neighborhood of the origin within the predefined-time, and the update frequency of control inputs exhibits a linear relationship with the predefined-time, avoiding Zeno behavior. Simulation results verify the effectiveness of the proposed method in complex marine environments. Compared with traditional control strategies, it achieves more accurate trajectory tracking, faster response, and a substantial reduction in control input update frequency, providing an efficient solution for the engineering implementation of embedded control systems in unmanned ships. Full article

Urochloa brizantha (Brizantha) is cultivated under varying altitudinal and management conditions. Twelve full-sun (monoculture) plots and twelve shaded (silvopastoral) plots were established, proportionally distributed at 170, 503, 661, and 1110 masl. Evaluations were conducted 15, 30, 45, 60, and 75 days after establishment. The conservation and integration of trees in silvopastoral systems reflected a clear anthropogenic influence, evidenced by the preference for species of the Fabaceae family, likely due to their multipurpose nature. Although the altitudinal gradient did not show direct effects on soil properties, intermediate altitudes revealed a significant role of CaCO₃ in enhancing soil fertility. These edaphic conditions at mid-altitudes favored the leaf area development of Brizantha, particularly during the early growth stages, as indicated by significantly larger values (p < 0.05). However, at the harvest stage, no significant differences were observed in physiological or productive traits, nor in foliar chemical components, underscoring the species’ high hardiness and broad adaptation to both soil and altitude conditions. In Brizantha, a significant reduction (p < 0.05) in stomatal size and density was observed under shade in silvopastoral areas, where solar radiation and air temperature decreased, while relative humidity increased. Nonetheless, these microclimatic variations did not lead to significant changes in foliar chemistry, growth variables, or biomass production, suggesting a high degree of adaptive plasticity to microclimatic fluctuations. Foliar ash content exhibited an increasing trend with altitude, indicating greater efficiency of Brizantha in absorbing calcium, phosphorus, and potassium at higher altitudes, possibly linked to more favorable edaphoclimatic conditions for nutrient uptake. Finally, forage quality declined with plant age, as evidenced by reductions in protein, ash, and In Vitro Dry Matter Digestibility (IVDMD), alongside increases in fiber, Neutral Detergent Fiber (NDF), and Acid Detergent Fiber (ADF). These findings support the recommendation of cutting intervals between 30 and 45 days, during which Brizantha displays a more favorable nutritional profile, higher digestibility, and consequently, greater value for animal feeding. Full article

Background: The candidate therapeutic peptide TnP demonstrates broad, system-level regulatory capacity, revealed through integrated network analysis from transcriptomic data in zebrafish. Our study primarily identifies TnP as a multifaceted modulator of drug metabolism, wound healing, proteolytic activity, and pigmentation pathways. Results: Transcriptomic profiling of TnP-treated larvae following tail fin amputation revealed 558 differentially expressed genes (DEGs), categorized into four functional networks: (1) drug-metabolizing enzymes (cyp3a65, cyp1a) and transporters (SLC/ABC families), where TnP alters xenobiotic processing through Phase I/II modulation; (2) cellular trafficking and immune regulation, with upregulated myosin genes (myhb/mylz3) enhancing wound repair and tlr5-cdc42 signaling fine-tuning inflammation; (3) proteolytic cascades (c6ast4, prss1) coupled to autophagy (ulk1a, atg2a) and metabolic rewiring (g6pca.1-tg axis); and (4) melanogenesis-circadian networks (pmela/dct-fbxl3l) linked to ubiquitin-mediated protein turnover. Key findings highlight TnP’s unique coordination of rapid (protease activation) and sustained (metabolic adaptation) responses, enabled by short network path lengths (1.6–2.1 edges). Hub genes, such as nr1i2 (pxr), ppara, and bcl6aa/b, mediate crosstalk between these systems, while potential risks—including muscle hypercontractility (myhb overexpression) or cardiovascular effects (ace2-ppp3ccb)—underscore the need for targeted delivery. The zebrafish model validated TnP-conserved mechanisms with human relevance, particularly in drug metabolism and tissue repair. TnP’s ability to synchronize extracellular matrix remodeling, immune resolution, and metabolic homeostasis supports its development for the treatment of fibrosis, metabolic disorders, and inflammatory conditions. Conclusions: Future work should focus on optimizing tissue-specific delivery and assessing genetic variability to advance clinical translation. This system-level analysis positions TnP as a model example for next-generation multi-pathway therapeutics. Full article

Fungi, from saprophytes to pathogens, face predictable daily fluctuations in light, temperature, humidity, and nutrient availability. To cope, they have evolved an internal circadian clock that confers a major adaptive advantage. This review critically synthesizes current knowledge on the molecular architecture and physiological relevance of fungal circadian systems, moving beyond the canonical Neurospora crassa model to explore the broader phylogenetic diversity of timekeeping mechanisms. We examine the core transcription-translation feedback loop (TTFL) centered on the FREQUENCY/WHITE COLLAR (FRQ/WCC) system and contrast it with divergent and non-canonical oscillators, including the metabolic rhythms of yeasts and the universally conserved peroxiredoxin (PRX) oxidation cycles. A central theme is the clock’s role in gating cellular defenses against oxidative, osmotic, and nutritional stress, enabling fungi to anticipate and withstand environmental insults through proactive regulation. We provide a detailed analysis of chrono-pathogenesis, where the circadian control of virulence factors aligns fungal attacks with windows of host vulnerability, with a focus on experimental evidence from pathogens like Botrytis cinerea, Fusarium oxysporum, and Magnaporthe oryzae. The review explores the downstream pathways—including transcriptional cascades, post-translational modifications, and epigenetic regulation—that translate temporal signals into physiological outputs such as developmental rhythms in conidiation and hyphal branching. Finally, we highlight critical knowledge gaps, particularly in understudied phyla like Basidiomycota, and discuss future research directions. This includes the exploration of novel clock architectures and the emerging, though speculative, hypothesis of “chrono-therapeutics”—interventions designed to disrupt fungal clocks—as a forward-looking concept for managing fungal infections. Full article

Corydalis yanhusuo W. T. Wang, commonly known as Yanhusuo, is an important and rare medicinal plant resource in China. Its habitat integrity is facing severe challenges due to climate change and human activities. Establishing an integrative quality zoning system for this species is of significant practical importance for resource conservation and adaptive management. This study integrates multiple data sources, including 121 valid distribution points, 37 environmental factors, future climate scenarios (SSP126 and SSP585 pathways for the 2050s and 2090s), and measured content of tetrahydropalmatine (THP) from 22 sampling sites. A predictive framework for habitat suitability and spatial distribution of effective components was constructed using a multi-model coupling approach (MaxEnt, ArcGIS spatial analysis, and co-kriging method). The results indicate that the MaxEnt model exhibits high prediction accuracy (AUC > 0.9), with the dominant environmental factors being the precipitation of the wettest quarter (404.8~654.5 mm) and the annual average temperature (11.8~17.4 °C). Under current climatic conditions, areas of high suitability are concentrated in parts of Central and Eastern China, including the Sichuan Basin, the middle–lower Yangtze plains, and coastal areas of Shandong and Liaoning. In future climate scenarios, the center of suitable areas is predicted to shift northwestward. The content of THP is significantly correlated with the mean diurnal temperature range, temperature seasonality, and the mean temperature of the wettest quarter (p < 0.01). A comprehensive assessment identifies the Yangtze River Delta region, Central China, and parts of the Loess Plateau as the optimal integrative quality zones. This research provides a scientific basis and decision-making support for the sustainable utilization of C. yanhusuo and other rare medicinal plants in China. Full article

Plant genetics and chemotaxonomic analysis are considered key parameters in understanding evolution, plant diversity and adaptation. Korean Peninsula has a unique biogeographical landscape that supports various aromatic plant species, each with considerable ecological, ethnobotanical, and pharmacological significance. This review aims to provide a comprehensive overview of the chemotaxonomic traits, biological activities, phylogenetic relationships and potential applications of Korean aromatic plants, highlighting their significance in more accurate identification. Chemotaxonomic investigations employing techniques such as gas chromatography mass spectrometry, high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy have enabled the identification of essential oils and specialized metabolites that serve as valuable taxonomic and diagnostic markers. These chemical traits play essential roles in species delimitation and in clarifying interspecific variation. The biological activities of selected taxa are reviewed, with emphasis on antimicrobial, antioxidant, anti-inflammatory, and cytotoxic effects, supported by bioassay-guided fractionation and compound isolation. In parallel, recent advances in phylogenetic reconstruction employing DNA barcoding, internal transcribed spacer regions, and chloroplast genes such as rbcL and matK are examined for their role in clarifying taxonomic uncertainties and inferring evolutionary lineages. Overall, the search period was from year 2001 to 2025 and total of 268 records were included in the study. By integrating phytochemical profiling, pharmacological evidence, and molecular systematics, this review highlights the multifaceted significance of Korean endemic aromatic plants. The conclusion highlights the importance of multidisciplinary approaches including metabolomics and phylogenomics in advancing our understanding of species diversity, evolutionary adaptation, and potential applications. Future research directions are proposed to support conservation efforts. Full article

Empirical and numerical methodologies for the geomechanical assessment of underground excavations have evolved in recent years to adapt to the geotechnical and structural conditions of natural caves, enabling stability evaluation and ensuring safe conditions for speleological exploration. This study analyzes the evolution of the state of the art of these techniques worldwide, assessing their reliability and application context, and identifying the most suitable methodologies for determining the stability of the Al-Badia lava tube. The research was conducted through bibliographic analysis and rock mass characterization using empirical geomechanical classifications. Subsequently, the numerical boundary element method (BEM) was applied to compare the obtained results and model the stress–strain behavior of the cavity. The results allowed the classification of the Al-Badia lava tube into stable, transition, and unstable zones, using empirical support charts and determining the safety factors of the surrounding rock mass. The study site highlights that empirical methods are rather conservative, and numerical results align better with observed conditions. Full article

African ground squirrels (Xerus spp.), the inhabitants of African arid zones, face extreme heat and water scarcity driving selection for metabolic optimization. We assembled and annotated the first mitogenomes of Xerus inauris and Xerus rutilus (16,525–16,517 bp), revealing conserved vertebrate architecture with genus-specific traits. Key features include Xerus rutilus’s elongated ATP6 (680 vs. 605 bp), truncated ATP8–ATP6 spacers (4 vs. 43 bp), and tRNA-Pro control regions with 78.1–78.3% AT content. Their nucleotide composition diverged from that of related sciurids, marked by reduced T (25.78–26.9%) and extreme GC skew (−0.361 to −0.376). Codon usage showed strong Arg-CGA bias (RSCU = 3.78–3.88) and species-specific elevations in Xerus rutilus’s UGC-Cys (RSCU = 1.83 vs. 1.17). Phylogenetics positioned Xerus as sister to Ratufa bicolor (Bayesian PP = 0.928; ML = 1.0), aligning with African biogeographic isolation. Critically, we identified significant signatures of positive selection in key mitochondrial genes linked to arid adaptation. Positive selection signals in ND4 (ω = 1.8 × background), ND1, and ATP6 (p < 0.0033) correspond to enhanced proton gradient efficiency and ATP synthesis–molecular adaptations likely crucial for optimizing energy metabolism under chronic water scarcity and thermoregulatory stress in desert environments. Distinct evolutionary rates were observed across mitochondrial genes and complexes: Genes encoding Complex I subunits (ND2, ND6) and Complex III (Cytb) exhibited accelerated evolution in arid-adapted lineages, while genes encoding Complex IV subunits (COXI) and Complex V (ATP8) remained highly conserved. These findings resolve the Xerus mitogenomic diversity, demonstrating adaptive plasticity balancing arid-energy optimization and historical diversification while filling critical genomic gaps for this xeric-adapted lineage. Full article

Understanding how habitat conditions drive morphological adaptations in animals is critical in ecology, yet amphibian studies remain limited. This study investigated intraspecific variation in ecomorphological traits of three montane stream-dwelling frogs (Quasipaa boulengeri, Amolops sinensis, and Odorrana margaratae) across elevation gradients in Tianping Mountain, China. Using morphological measurements and environmental variables collected from ten transects, we analyzed functional traits related to feeding and locomotion and assessed their associations with microhabitat variables. Significant trait differences between low- and high-elevation groups were detected only in Q. boulengeri, with high-elevation individuals exhibiting greater body mass and shorter hindlimbs. Redundancy analysis demonstrated that microhabitat variables, particularly air humidity, flow rate, and rock coverage, were linked to trait variations. For example, air humidity and flow rate significantly influenced Q. boulengeri’s body and limb proportions, while flow rate affected A. sinensis’s snout and limb morphology. In addition, sex and seasonal effects were also associated with trait variations. These results underscore amphibians’ phenotypic plasticity in response to the environment and highlight the role of microhabitat complexity in shaping traits. By linking habitat heterogeneity to eco-morphology, this study advocates for conservation strategies that preserve varied stream environments to support amphibian resilience amid environmental changes. Full article

Anthocyanins, crucial water-soluble pigments in plants, determine coloration in floral and fruit tissues, while fulfilling essential physiological roles in terms of plant growth, development, and stress adaptation. The biosynthesis of anthocyanins is transcriptionally regulated by WRKY factors, one of the largest plant-specific transcription factor families. Euscaphis japonica is an East Asian species, prized for its exceptionally persistent butterfly-shaped fruits that undergo pericarp dehiscence, overturning, and a color transition to scarlet red. This species represents an ideal system for studying anthocyanin regulation. However, the mechanisms by which WRKY transcription factors orchestrate anthocyanin accumulation during this process remain unknown. In this study, we identified 87 WRKY genes (EjaWRKYs) from the E. japonica genome. Phylogenetic analysis was used to classify these genes into three primary groups, with five subgroups, revealing conserved gene structures and motif compositions, supported by collinearity and comparative synteny analyses. Crucially, ten EjaWRKYs exhibited peak expression during the mature fruit stages, showing positive correlations with key anthocyanin biosynthesis genes. Functional validation through the use of transient transactivation assays in Nicotiana benthamiana confirmed that the five selected EjaWRKYs bind W-box elements and strongly activate reporter gene expression. Our results reveal EjaWRKYs’ regulation of anthocyanin accumulation in E. japonica fruit, provide the first comprehensive WRKY family characterization of this species, and establish a foundation for manipulating ornamental traits in horticultural breeding. Full article