Search Results (947)

As the demand for sustainable materials continues to grow, calcium caseinate (Cas) biopolymer films have emerged as promising alternatives to fossil-based plastics. However, their mechanical fragility and high-water sensitivity limit their application in packaging. In this study, we reinforced Cas films with zinc hydroxide nitrate (ZHN) using two incorporation methods: wet (ZHN-w) and dry (ZHN-d). We evaluated how each method affected the dispersion of the filler and, consequently, the functional properties of the films. To our knowledge, this is the first report of ZHN being used in biopolymeric films. Structural and morphological analyses showed better dispersion of ZHN in the wet-incorporated films. These samples exhibited a substantial increase in tensile strength, from 0.75 ± 0.00 MPa to 9.62 ± 2.45 MPa, along with a marked improvement in Young’s modulus. The films also became less soluble in water, more resistant to swelling, and structurally more cohesive. In antimicrobial tests, the ZHN-w films showed stronger inhibition against E. coli and S. aureus. Overall, this approach offers a simple and effective way to enhance protein-based films using food-safe materials, making them suitable for active and bio-based packaging applications. Full article

To improve the postharvest preservation of cherry tomatoes and combat pathogenic, both bacterial and fungal contamination (particularly Alternaria alternata), a novel biodegradable coating was developed based on a water-in-water (W/W) Pickering emulsion system. The emulsion was stabilized by L. plantarum (Lactobacillus plantarum), with maltodextrin (MD) as the dispersed phase and hydroxypropyl methylcellulose (HPMC) as the continuous phase. Characterization of emulsions at varying concentrations revealed that the optimized W/W-PL^8 film exhibited superior stability, smooth morphology, and low water vapor permeability (WVP = 220.437 g/(m²·24 h)), making it a promising candidate for fruit and vegetable preservation. Furthermore, the coating demonstrated strong antioxidant activity, with scavenging rates of 58.99% (ABTS) and 94.23% (DPPH), along with potent antimicrobial effects, showing inhibition rates of 12.8% against Escherichia coli and 23.7% against Staphylococcus aureus. Applied to cherry tomatoes, the W/W-PL^8 coating significantly reduced respiration rates, minimized decay incidence, and maintained nutritional quality during storage. Remarkably, the coating successfully controlled Alternaria alternata contamination, enhancing the storage duration of cherry tomatoes. These findings highlight the potential of W/W-PL^8 as an eco-friendly and functional packaging material for fresh produce preservation. Full article

In recent years, active packaging has become a focal point of research and development in the food industry, driven by increasing consumer demand for safe, high-quality, and sustainable food products. In this work, solvent casting processed an active antibacterial multicomponent film based on hydroxypropyl methylcellulose incorporated with ferulic acid and chitin nanofibers. The influences of ferulic acid and different content of chitin nanofibers on the structure, thermal, mechanical, and water vapor stability and antioxidant and antibacterial efficiency of films were studied. It was shown that the inclusion of only ferulic acid did not significantly influence the mechanical, water vapor, and thermal stability of films. In addition, films containing only ferulic acid did not display antibacterial activity. The optimal concentration of chitin nanofibers in hydroxypropyl methylcellulose–ferulic acid films was 5 wt%, providing a tensile strength of 15 MPa, plasticity of 52%, and water vapor permeability of 0.94 × 10⁻⁹ g/m s Pa. With further increase of chitin nanofibers content, films with layered and discontinuous phases are obtained, which negatively influence tensile strength and water vapor permeability. Moreover, only films containing both ferulic acid and chitin nanofibers demonstrated antibacterial activity toward E. coli and S. aureus, suggesting that the presence of fibers allows easier release of ferulic acid from the matrix. These results imply that the investigated three-component systems have potential applicability as sustainable active food packaging materials. Full article

Antimicrobial food packaging with natural antimicrobials and biodegradable polymers presents an innovative solution to mitigate microbial contamination, prolong freshness, reduce food waste, and alleviate environmental burden. This study developed antimicrobial hemicellulose-based films by incorporating carvacrol (1% and 2%) as a natural antimicrobial agent through micro-emulsification produced by high-pressure homogenization (M-films). For comparison, films with the same formula were constructed using coarse emulsions (C-films) without high-pressure homogenization. These films were investigated for their antimicrobial efficacy, mechanical and barrier properties, and physicochemical attributes to explore their potential as sustainable antimicrobial packaging solutions. The M-films demonstrated superior antimicrobial activity, achieving reductions exceeding 4 Log CFU/mL against Listeria monocytogenes, Escherichia coli, and Salmonella enterica, compared to the C-films. High-pressure homogenization significantly reduced the emulsion’s particle size, from 11.59 to 2.55 μm, and considerably enhanced the M-film’s uniformity, hydrophobicity, and structural quality. Most importantly, the M-films exhibited lower oxygen transmission (35.14 cc/m²/day) and water vapor transmission rates (52.12 g/m²/day) than the C-films at 45.1 and 65.5 cc/m²/day, respectively, indicating superior protection against gas and moisture diffusion. Markedly improved mechanical properties, including foldability, toughness, and bubble-free surfaces, were also observed, making the M-films suitable for practical applications. This study highlights the potential of high-pressure homogenization as a method for enhancing the functional properties of hemicellulose-based films (i.e., M-films). The fabricated films offer a viable alternative to conventional plastic packaging, paving the way for safer and greener solutions tailored to modern industry needs. Full article

In order to develop modern polymer films intended for food packaging, materials based on cellulose acetate propionate (CAP) with the addition of Tween 80 as a plasticizer and cinnamic acid (CA), known for its antibacterial properties, were prepared. It should be emphasized that materials based on CAP combined with Tween 80 have not been previously reported in the literature. Therefore, not only is the incorporation of cinnamic acid into these systems an innovative approach, but also the use of the CAP-Tween80 matrix itself represents a novel strategy in the context of the proposed applications. The conducted studies made it possible to assess the properties of the obtained materials with and without the addition of cinnamic acid. The obtained results showed that the addition of cinnamic acid significantly influenced the crucial properties relevant to food storage. The introduction of CA into the polymer matrix notably enhanced the UV barrier properties achieving complete (100%) blockage of UVB radiation and approximately a 20% reduction of UVA transmittance. Furthermore, the modified films exhibited pronounced antibacterial activity, with over 99% reduction in Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa populations observed for samples containing 2 and 3% CA. This antibacterial effect contributed to the extended freshness of stored blueberries. Moreover, the addition of cinnamic acid did not significantly affect the transparency of the films, which remained high (97–99%), thereby allowing the fruit to remain visible. Full article

In this study, a composite film with dual antioxidant and antibacterial properties was prepared by combining 2% chitosan and 7% gelatin (2:1, w:w), with D-carvone (0–4%) as the primary active component. The effect of D-carvone content on the performance of the composite films was systematically investigated. The results showed that adding 1% D-carvone increased the water contact angle by 28%, increased the elongation at break by 35%, and decreased the WVTR by 18%. FTIR and SEM confirmed that ≤2% D-carvone uniformly bonded with the substrate through hydrogen bonds, and the film was dense and non-porous. In addition, the DPPH scavenging rate of the 1–2% D-carvone composite film increased to about 30–40%, and the ABTS⁺ scavenging rate increased to about 35–40%; the antibacterial effect on Escherichia coli and Staphylococcus aureus increased by more than 70%. However, when the addition amount was too high (exceeding 2%), the composite film became agglomerated, microporous, and phase-separated, affecting the film performance, and due to its own taste, it reduced the sensory quality of the noodles. Comprehensively, the composites showed better performance when the content of D-carvone was 1–2% and also the best effect for freshness preservation in Xinjiang ramen. This study provides a broad application prospect for natural terpene compound-based composite films in the field of high-moisture, multi-fat food preservation, and provides a theoretical basis and practical guidance for the development of efficient and safe food packaging materials. In the future, the composite film can be further optimized, and the effect of flavor can be further explored to meet the needs of different food preservation methods. Full article

The need for renewable and eco-friendly materials is driving the increasing demand for biobased polymers for food applications, with cellulose emerging as a promising option due to its degradability and environmental sustainability. Therefore, in the present study, a strategy to obtain cellulose-based materials with antimicrobial properties was explored by using a selected antimicrobial peptide named RKT1, which was stably and efficiently tethered to cellulose films via physical adsorption, harnessing the high number of functional groups on the polymeric surface. Firstly, the peptide, identified among the previous or new projected compounds, was structurally and functionally characterized, evidencing high conformational stability under a wide range of environmental conditions and efficient antibacterial activity against the foodborne pathogens Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes and the spoilage bacteria Enterococcus and Pseudomonas koreensis, all isolated from meat products. Moreover, in an extended application, the RKT1-activated cellulose films were tested in vivo on beef carpaccio. The results supported their effectiveness in increasing the shelf life of carpaccio by least two days without affecting its organoleptic properties. Therefore, RKT1, physically adsorbed on cellulose, still retains its activity, and the newly generated biopolymers show potential for use as a green food packaging material. Full article

We present a neural-network-based approach to solve the image-to-image translation problem in microscale droplets squeeze flow. A residual convolutional neural network is proposed to address the inverse problem: reconstructing a low-resolution (LR) droplet pattern image from a high-resolution (HR) liquid film thickness imprint. This enables the prediction of initial droplet configurations that evolve into target HR imprints after a specified spreading time. The developed neural network architecture aims at learning to tune the refinement level of its residual convolutional blocks by using function approximators that are trained to map a given film thickness to an appropriate refinement level indicator. We use multiple stacks of convolutional layers, the output of which is translated according to the refinement level indicators provided by the directly connected function approximators. Together with a non-linear activation function, the translation mechanism enables the HR imprint image to be refined sequentially in multiple steps until the target LR droplet pattern image is revealed. We believe that this work holds value for the semiconductor manufacturing and packaging industry. Specifically, it enables desired layouts to be imprinted on a surface by squeezing strategically placed droplets with a blank surface, eliminating the need for customized templates and reducing manufacturing costs. Additionally, this approach has potential applications in data compression and encryption. Full article

The present study aimed to develop biodegradable films formulated with pectin/carboxymethyl cellulose (CMC) and cinnamon essential oil, investigating the effects of CP treatment time on the properties of the films. The developed films were used as packaging to evaluate the shelf life of chicken meat. Biodegradable films were produced from a film-forming solution containing pectin/CMC, glycerol (30%), and cinnamon essential oil (2%). All formulations included the essential oil, and the control group corresponded to the film that was not subjected to CP treatment. The CP treatments were applied at 22.5 L/min, 20 kV, and 80 kHz for 10, 20, and 30 min. The results showed that increasing CP treatment time led to a progressive reduction in apparent viscosity, indicating improved homogeneity of the polymer system. Hydrophobicity increased with treatment time, as shown by a higher contact angle (from 51.15° to 62.38°), resulting in lower water solubility. Mechanical properties were also enhanced, with tensile strength rising from 3.29 MPa to 6.74 MPa after 30 min of CP. Biodegradability improved with treatment time, reaching 99.51% mass loss after 15 days for the longest exposure. Films produced from the solution treated for 30 min (FCP30) were most effective in extending the shelf life of chicken breast fillets, reducing lipid oxidation (TBARS: 61.9%), peroxide content (58.7%), and microbial spoilage (TVB-N: 59.2%) compared to the untreated film. Overall, the results highlight the importance of CP treatment time as a key factor in enhancing film performance, supporting its application in sustainable active packaging. Full article

A fungal strain with comparably high chitosan yield was isolated from the Shivaganga hills and identified as Aspergillus versicolor AD07 through molecular characterization. Later, the strain was cultivated on Sabouraud Dextrose Broth (SDB) and wild jackfruit-based media to evaluate its potential for chitosan production. Among the various media formulations, the highest chitosan yield (178.40 ± 1.76 mg/L) was obtained from the jackfruit extract medium with added peptone and dextrose. The extracted chitosan was characterized through FTIR, XRD (reported a crystallinity index of 55%), TGA/DTG, and DSC analysis, confirming the presence of key functional groups and high thermal resistance. The extracted chitosan was fabricated into a sheet incorporated with 1% lemongrass oil; the sheet exhibited strong antibacterial activity against Escherichia coli (30 mm) and Bacillus megaterium (48 mm). The biodegradation studies reported a weight loss of 38.93 ± 0.51% after 50 days of soil burial. Further, the chitosan film was tested as a packaging material for paneer, demonstrating better preservation by maintaining nutritional quality and reducing microbial load over a 14-day storage period. These findings highlight the potential of A. versicolor AD07-derived chitosan, cultivated on a waste substrate medium, as a sustainable biopolymer for food packaging applications. Full article

This study presented a novel antimicrobial packaging PVA/xanthan gum film decorated with green-synthesized silver nanoparticles (AgNPs) derived from Myrica rubra leaf extract (MRLE) for the first time. Montmorillonite (MMT) was used to improve its dispersion (AgNPs@MMT). The synthesis time, temperature, and concentration of AgNO₃ were considered using a central composite design coupled with response surface methodology to obtain the optimum AgNPs (2 h, 75 °C, 2 mM). Analysis of substance concentration changes confirmed that the higher phenolic and flavonoid content in MRLE acted as reducing agents and stabilizers in AgNP synthesis, participating in the reaction rather than adsorbing to nanoparticles. TEM, XRD, and FTIR images revealed a spherical shape of the prepared AgNPs, with an average diameter of 8.23 ± 4.27 nm. The incorporation of AgNPs@MMT significantly enhanced the mechanical properties of the films, with the elongation at break and shear strength increasing by 65.19% and 52.10%, respectively, for the PAM2 sample. The films exhibited strong antimicrobial activity against both Escherichia coli (18.56 mm) and Staphylococcus aureus (20.73 mm). The films demonstrated effective food preservation capabilities, significantly reducing weight loss and extending the shelf life of packaged grapes and bananas. Molecular dynamics simulations reveal the diffusion behavior of AgNPs in different matrices, while the measured silver migration (0.25 ± 0.03 mg/kg) complied with EFSA regulations (10 mg/kg), confirming its food safety. These results demonstrate the film’s potential as an active packaging material for fruit preservation. Full article

Buckwheat, a dicotyledonous pseudocereal from the Polygonaceae family, has emerged as a crop of scientific and industrial interest due to its exceptional phytochemical profile, adaptability to different environments, and minimal agronomic input requirements. This paper aims to highlight the proximate composition (carbohydrates, protein, dietary fiber, lipids, starch, vitamins, and minerals) of the buckwheat principal species, Fagopyrum esculentum Moench (common buckwheat) and Fagopyrum tataricum (L.) Gaertn (Tartary buckwheat). Other bioactive compounds, including flavonoids (e.g., rutin, quercetin), phenolic acids, and anthocyanins, were emphasized, together with their influence on human health. These constituents confer a broad range of biological activities such as anti-inflammatory, antimicrobial, antidiabetic, antihypertensive, and hypoglycemic effects. Moreover, buckwheat is inherently gluten-free, making it a valuable alternative in formulations targeting gluten-sensitive populations. Finally, the review addresses the possibility of using starch buckwheat as a raw material in starch-based films. Further research is needed to elucidate the potential of buckwheat starch as a viable material for the development of biodegradable food packaging films. Full article

This study investigates the combined use of electron beam irradiation (EBI) and nanotechnology to develop improved food packaging films. EBI, commonly applied for sterilization, can alter polymer microstructure, while irradiated cellulose nanocrystals (CNCs) offer enhanced functionality when incorporated into biopolymer matrices. Here, CNCs were irradiated with doses up to 50 kGy, leading to the formation of carboxyl and aldehyde groups, confirmed by FTIR analysis, as a consequence of the initial formation of free radicals and peroxides that may subsist in that original form or be converted into various carbonyl groups. Flexible films were obtained by incorporating pristine and EB-irradiated CNCs in an internal mixer, using minute amounts of poly(ethylene oxide) (PEO) to facilitate the dispersion of the filler within the polymer matrix. The resulting PLA/PEO/CNC films were evaluated for their mechanical, thermal, barrier, and antioxidant properties. The results showed that structural modifications of CNCs led to significant enhancements in the performance of the composite films, including a 30% improvement in water barrier properties and a 50% increase in antioxidant activity. These findings underscore the potential of irradiated CNCs as effective additives in biopolymer-based active packaging, offering a sustainable approach to reduce dependence on synthetic preservatives and potentially extend the shelf life of food products. Full article

Spice by-products, often discarded as waste, represent an untapped resource for sustainable packaging solutions due to their unique, multifunctional, and bioactive profiles. Unlike typical plant residues, these materials retain diverse phytochemicals—including phenolics, polysaccharides, and other compounds, such as essential oils and vitamins—that exhibit controlled release antimicrobial and antioxidant effects with environmental responsiveness to pH, humidity, and temperature changes. Their distinctive advantage is in preserving volatile bioactives, demonstrating enzyme-inhibiting properties, and maintaining thermal stability during processing. This review encompasses a comprehensive characterization of phytochemicals, an assessment of the re-utilization pathway from waste to active materials, and an investigation of processing methods for transforming by-products into films, coatings, and nanoemulsions through green extraction and packaging film development technologies. It also involves the evaluation of their mechanical strength, barrier performance, controlled release mechanism behavior, and effectiveness of food preservation. Key findings demonstrate that ginger and onion residues significantly enhance antioxidant and antimicrobial properties due to high phenolic acid and sulfur-containing compound concentrations, while cinnamon and garlic waste effectively improve mechanical strength and barrier attributes owing to their dense fiber matrix and bioactive aldehyde content. However, re-using these residues faces challenges, including the long-term storage stability of certain bioactive compounds, mechanical durability during scale-up, natural variability that affects standardization, and cost competitiveness with conventional packaging. Innovative solutions, including encapsulation, nano-reinforcement strategies, intelligent polymeric systems, and agro-biorefinery approaches, show promise for overcoming these barriers. By utilizing these spice by-products, the packaging industry can advance toward a circular bio-economy, depending less on traditional plastics and promoting environmental sustainability in light of growing global population and urbanization trends. Full article

The aim of this study was to develop and characterize antioxidant-active films for potential food packaging applications. The films were produced by casting aqueous solutions containing semolina flour (6% w/w), pectin extracted from passion fruit (1% w/w), inverted sugar (1% w/w), and sucrose (1% w/w), incorporating hydroalcoholic extracts from jatoba stem bark (X₁) and pods (X₂) at concentrations ranging from 0 to 1% (w/w). The films were characterized in terms of their functional, physical, chemical, structural, and degradation properties. The formulation that showed the best performance, referred to as the optimized formulation (FO), contained 0.5% X₁ and 0.5% X₂, presenting a high phenolic compound content (8.80 mg GAE/g), strong antioxidant activity as determined by the DPPH method (75.28%) and FRAP assay (6.02 mmol FeSO₄/g), good thermal stability (350 °C), and a high soil degradation rate (83.47% in 15 days). These results indicate that the FO film has potential application as a primary packaging material with antioxidant function for oxidation-sensitive foods, meeting the demand for biodegradable and environmentally sustainable solutions in the food industry. Full article