Search Results (811)

The study of energy needs in rural areas continues to be an active field of research. Although numerous gaps hinder the achievement of a sustainable energy transition in these areas, it is necessary to develop comprehensive strategies that integrate local participation with the implementation of efficient and appropriate energy technologies. This research analyzes local energy needs using a community participatory approach and considers four main stages, including a participatory diagnosis at the community level to identify energy needs, defining priority energy needs from the community’s viewpoint, estimating a baseline of the identified needs, their economic costs, and environmental impacts, constructing a scenario with a 20-year projection, and the benefits of implementing more efficient technologies. The results show that 98.9% of energy is destined for residential needs, 0.6% for community needs, and 0.5% for productive needs, and the economic expenditure follows the same hierarchy, while total emissions are estimated annually at just over 30,000 tCO_2e and 3 tPM_2.5. With the proposed scenario, at the end of year 20, a reduction in consumption of just over 200 TJ is estimated, together with present value savings of USD 490,000, and a decrease in emissions of approximately 27,000 tCO_2e and 2.7 tPM_2.5. This proposal is expected to contribute to encouraging research with broad community participation and to the formulation of strategies that enable a sustainable energy transition in rural contexts. Full article

Brown adipose tissue (BAT) and beige adipocytes play a crucial role in adaptive thermogenesis, primarily via uncoupling protein 1 (UCP1)-driven heat production. Once considered physiologically irrelevant in adults, BAT is now recognized as an active tissue that contributes to energy expenditure and metabolic homeostasis and represents a potential therapeutic target for obesity and metabolic disorders. This review provides an integrated overview of the molecular regulation of thermogenic adipocytes, emphasizing both canonical UCP1-dependent as well as non-canonical UCP1-independent mechanisms of heat generation. Key transcriptional and epigenetic regulators are discussed in the context of mitochondrial biogenesis, substrate utilization, and thermogenic gene programs. Major upstream signaling routes are further summarized, encompassing classical β-adrenergic pathways, as well as alternative regulatory nodes including AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR) together with diverse nutrient- and hormone-responsive cues that converge to activate brown and beige adipocytes. Finally, the cross-talk among neuronal, endocrine, immune, and gut microbiota-derived signals is highlighted as a key determinant of thermogenic adipocyte function. Together, these multilayered regulatory inputs provide a comprehensive framework for understanding how thermogenic adipose tissue integrates environmental, metabolic, and microbial cues to regulate systemic energy balance—knowledge that is essential for developing targeted therapies to combat obesity and metabolic diseases. Full article

Obesity has reached epidemic proportions, driven by energy imbalance and limited capacity for adaptive thermogenesis. Brown (BAT) and beige adipose tissues dissipate energy through non-shivering thermogenesis (NST), primarily via uncoupling protein-1 (UCP1), making them attractive targets for increasing energy expenditure (EE). The canonical β-adrenergic pathway robustly activates NST in rodents through β3 adrenoceptors; however, translational success in humans has been limited by low β3 expression, off-target cardiovascular effects, and the emerging dominance of β2-mediated signaling in human BAT. Consequently, attention has shifted to non-adrenergic and UCP1-independent mechanisms that offer greater tissue distribution and improved safety profiles. This review examines a broad spectrum of alternative receptors and pathways—including GPRs, TRP channels, TGR5, GLP-1R, thyroid hormone receptors, estrogen receptors, growth hormone, BMPs, sirtuins, PPARs, and interleukin signaling—as well as futile substrate cycles (Ca²⁺, creatine, and glycerol-3-phosphate) that sustain thermogenesis in beige adipocytes and skeletal muscle. Pharmacological agents (natural compounds, peptides, and small molecules) and non-pharmacological interventions (cold exposure, exercise, diet, and time shift) targeting these pathways are critically evaluated. We highlight the translational gaps between rodent and human studies, the promise of multimodal therapies combining low-dose adrenergic agents with non-adrenergic activators, and emerging strategies such as sarco/endoplasmic reticulum calcium ATPase protein (SERCA) modulators and tissue-specific delivery. Ultimately, integrating adrenergic and non-adrenergic approaches holds the greatest potential for safe, effective, and sustainable obesity management. Full article

Metabolic reprogramming allows cancer cells to proliferate rapidly, survive nutrient limitation, and resist stress, making tumor metabolism an important therapeutic target. However, pharmacological inhibition of metabolic enzymes often causes systemic toxicity and compensatory pathway activation. To overcome these limitations, recent studies have highlighted an alternative host-centered strategy based on increasing systemic energy expenditure. Recent studies highlight an alternative strategy in which the host increases energy expenditure through uncoupling protein 1 (UCP1) dependent thermogenesis, thereby lowering systemic glucose, fatty acid, and nucleotide availability for tumors. Engineered beige adipocytes overexpressing UCP1, PR domain-containing protein 16 (PRDM16), or peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PPARGC1A/PGC1A) suppress tumor growth through nutrient competition, suggesting that activating endogenous UCP1 may provide a non-genetic and physiologically aligned anticancer approach. Building on this concept, natural products such as polyphenols, terpenoids, alkaloids, and carotenoids have emerged as promising UCP1 activators that stimulate beige and brown adipocyte thermogenesis through pathways involving AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1), PGC1A, PRDM16, and mitochondrial biogenesis. In parallel, computational studies further indicate that several plant-derived compounds bind directly to the central cavity of UCP1 with high affinity, offering structural support for their thermogenic action. Importantly, many of these compounds also inhibit cancer cell intrinsic metabolism by reducing glycolysis, oxidative phosphorylation, lipid synthesis, and amino acid dependent anaplerosis. This review integrates UCP1 biology, natural product mediated thermogenesis, molecular docking evidence, and tumor metabolic suppression, proposing a unified framework in which natural compounds impose coordinated metabolic pressure on cancer through both adipocyte-driven nutrient competition and direct inhibition of tumor metabolism. Full article

Adipose tissue is far more than a passive reservoir for surplus energy: it is an active metabolic and endocrine organ that senses nutrient availability and orchestrates systemic energy balance. When caloric intake chronically exceeds expenditure, adipocytes become engorged with lipids and exposed to metabolic, mechanical, and hypoxic stress. To adapt, they initiate a fibro-inflammatory response that may be protective in the short term. As this response becomes chronic, adipocytes lose their metabolic flexibility, acquire a maladaptive fibro-inflammatory phenotype, and contribute to the cascade of inflammation, insulin resistance, and metabolic disease that characterizes obesity. In this review, we dissect the cellular and molecular cues that trigger fibro-inflammation, from nutrient excess and mitochondrial stress to hypoxia and immunometabolic rewiring, and highlight how these processes reshape adipocyte identity and tissue homeostasis. Full article

Nobiletin, a citrus-derived polymethoxylated flavone, has been reported to exert anti-obesity effects, but its molecular mechanisms remain poorly understood. This study aimed to investigate whether nobiletin suppresses adipogenesis and promotes browning in 3T3-L1 adipocytes by modulating exosomal microRNAs (miRNAs) and AMPK signaling. To this end, we treated 3T3-L1 adipocytes with various concentrations of nobiletin and evaluated gene and protein expression by RT-qPCR and Western blotting. Nobiletin significantly reduced intracellular lipid accumulation at 50 μM (p < 0.001) and downregulated key adipogenic transcription factors, PPARγ, C/EBPα, and SREBP-1c, and suppressed the lipogenic enzyme FAS, while activating the AMPK/ACC signaling pathway. Concomitantly, it enhanced the expression of thermogenic markers UCP-1, PRDM16, and PGC-1α, indicating a metabolic shift toward energy expenditure. Exosomal RNA-seq revealed 10 differentially expressed miRNAs, of which miR-181d-5p (3.1-fold) and miR-221-3p (2.4-fold) were upregulated, whereas miR-205-5p (−2.9-fold), miR-331-3p (−3.2-fold), miR-130b-3p (−2.6-fold), miR-143-5p (−2.9-fold), miR-183-3p (−2.8-fold), miR-196b-5p (−2.4-fold), miR-26b-3p (−2.2-fold), and miR-378d (−2.7-fold) were verified by RT-qPCR after nobiletin treatment (50 μM). These miRNAs are functionally associated with adipogenic and thermogenic pathways, supporting a regulatory role of the exosomal miRNA network in nobiletin’s action. Collectively, our results identify a novel exosome–miRNA–AMPK axis underlying the anti-adipogenic and browning-inducing activities of nobiletin, highlighting its potential as a therapeutic phytochemical for obesity prevention. Full article

This study proposes a scalable cyber–physical system (CPS) framework utilizing a hierarchical five-layer architecture to enhance indoor environmental quality and energy efficiency. The methodology integrates a Random Forest-based predictive model trained on a 22-month longitudinal dataset (2024–2025) to separate climatic effects from occupancy-driven loads. This study prioritized the development of a high-precision and cost-effective monitoring architecture to address the persistent challenge of sustaining thermal comfort in subtropical academic laboratories. The proposed system achieved a validation mean absolute percentage error (MAPE) of 2.50%, indicating strong predictive reliability. Hardware expenditures were below USD 400, substantially reducing barriers to broader adoption. Field deployment confirmed an operational EUI of 188.6 kWh/m²·year, which is 28.5% lower than prevailing regional benchmarks, while consistently meeting stringent indoor air quality (IAQ) requirements. Additionally, simulation modules calibrated with the validated dataset indicated a further 15–20% reduction potential through the application of active control strategies. Collectively, these findings establish a transferable empirical reference for climate-responsive operational practice. Full article

Background: Reserve Officer Training Corps (ROTC) Cadets undergo biannual Field Training Exercises (FTX) that impose substantial physiological demands, necessitating adequate nutritional intake to support performance and recovery. Methods: Energy Expenditure (EE) measured by actigraphy and self-reported nutritional intake (NI) of ROTC Cadets during a Fall FTX were obtained and compared to Military Dietary Reference Intake (MDRI) guidelines. Energy balance and nutrient adequacy were assessed using paired sample t-tests. Results: Cadets demonstrated significant caloric deficits, consuming fewer kilocalories than both their active metabolic rate (t = −12.07, df = 42, p < 0.001) and Low Energy Availability thresholds (t = 6.47, df = 57.54, p < 0.001). Macronutrient analysis revealed widespread deficiencies. Neither male nor female cadets met minimum carbohydrate gram recommendations. Protein intake in grams was significantly below MDRI guidelines for 94% of males (t = −10.03, p < 0.001) and 90% of females (t = −4.62, p = 0.001). Fat intake was generally adequate for all cadets, with 94% of males (t = 6.50, p < 0.001) and 90% of females (t = 4.19, p = 0.002) meeting or exceeding recommended fat intake. Conclusions: These findings underscore the prevalence of under-fueling during FTX and highlight the need for improved nutritional strategies to mitigate energy deficits and support cadet performance and health. Full article

With the rapid growth of residential electric vehicles, synchronized charging during peak periods can induce severe load ramping and exceed distribution network capacity limits. To mitigate these issues, governments have promoted a unified build-and-operate community model that enables centralized coordination of community charging and ensures real-time responsiveness to grid dispatch signals. Targeting this emerging operational paradigm, a dual-dimensional compensation mechanism for real-time electric vehicle (EV) demand response is proposed. The mechanism integrates two types of compensation: power regulation compensation, which rewards users for providing controllable power flexibility, and state-of-charge (SoC) loss compensation, which offsets energy deficits resulting from demand response actions. This dual-layer design enhances user willingness and long-term engagement in community-level coordination. Based on the proposed mechanism, a bi-level optimization framework is developed to realize efficient real-time regulation: the upper level maximizes the active response capacity under budget constraints, while the lower level minimizes the aggregator’s total compensation cost subject to user response behavior. Simulation results demonstrate that, compared with conventional fair-share curtailment and single-compensation approaches, the proposed mechanism effectively increases active user participation and reduces incentive expenditures. The study highlights the mechanism’s potential for practical deployment in unified build-and-operate communities and discusses limitations and future research directions. Full article

This study examines the short- and medium-run linkages within Mexico’s energy–economy system under conditions of limited information. The analysis is motivated by the structural relevance of hydrocarbons for fiscal stability and by the growing need to understand how energy shocks propagate through economic and environmental subsystems. Using a vector autoregression (VAR) framework, nine interdependent macroeconomic and energy variables are jointly evaluated after harmonizing mixed-frequency data, standardizing series, and ensuring stationarity through ADF and KPSS tests. Dynamic responses are assessed through impulse response functions (IRFs), generalized IRFs (GIRFs), and forecast error variance decomposition (FEVD), complemented by Granger causality tests. Results show that oil rents exert a persistent and positive influence on GDP and public expenditure, while shocks to coal-fired generation and oil prices consistently reduce economic activity and increase emissions. Renewable capacity expands pro-cyclically but displays limited autonomous effects. Overall, the evidence reveals a fiscally and environmentally constrained system dominated by hydrocarbons, underscoring the importance of improving PEMEX’s operational efficiency, accelerating fiscal diversification, and strengthening institutional conditions for renewable investment. Full article

Background: This scoping review systematically maps and synthesises contemporary literature on the biomechanics of active below-knee prosthetic devices, focusing on gait kinematics, kinetics, energy expenditure, and muscle activation. It further evaluates design advancements, including powered ankle–foot prostheses and variable impedance systems, that seek to emulate physiological ankle function and enhance mobility outcomes for transtibial amputees. Methods: This review followed the PRISMA-ScR guidelines. A comprehensive literature search was conducted on ScienceDirect, PubMed and IEEE Xplore for studies published between 2013 and 2023. Search terms were structured according to the Population, Intervention, Comparator, and Outcome (PICO) framework. From 971 identified articles, 27 peer-reviewed studies were found to meet the inclusion criteria between January 2013 and December 2023. Data were extracted on biomechanical parameters, prosthetic design characteristics, and participant demographics to identify prevailing trends and research gaps. This scoping review was registered with Research Registry under the following registration number: reviewregistry 2055. Results: The reviewed studies demonstrate that active below-knee prosthetic systems substantially improve gait symmetry and ankle joint range of motion compared with passive devices. However, compensatory trunk and pelvic movements persist, indicating that full restoration of natural gait mechanics remains incomplete. Metabolic efficiency varied considerably across studies, influenced by device design, control strategies, and user adaptation. Notably, the literature exhibits a pronounced gender imbalance, with only 10.7% female participants, and a reliance on controlled laboratory conditions, limiting ecological validity. Conclusions: Active prosthetic technologies represent a significant advancement in lower-limb rehabilitation. Nevertheless, complete biomechanical normalisation has yet to be achieved. Future research should focus on long-term, real-world evaluations using larger, more diverse cohorts and adaptive technologies such as variable impedance actuators and multi-level control systems to reduce asymmetrical loading and optimise gait efficiency. Full article

Background/Objectives: Intermittent hypobaric hypoxia (IHH) induces various physiological and metabolic adaptations. This study aimed to investigate the effects of a seven-day IHH exposure on nutritional status, body composition, gut microbiota, movement intensity, and energy expenditure in 10 workers. Methods: A pre–post comparative design was employed, with measurements taken at the beginning and end of the exposure period. Nutritional status, body composition, and phase angle (PhA) were assessed via bioelectrical impedance analysis (BIA). Gut microbiota composition was analyzed through fecal DNA extraction and qPCR for specific bacterial families. Movement intensity and energy expenditure were monitored using accelerometry. An initial statistical analysis was performed, which included paired t-tests and Wilcoxon signed-rank tests. Results: A significant increase in PhA (mean difference: 0.40; p = 0.0053 for t-test, p = 0.0136 for Wilcoxon) and a significant decrease in BMI (mean difference: −0.38; p = 0.0311 for t-test, p = 0.0546 for Wilcoxon). Conclusions: While the original paper reported no significant changes in nutritional status or body composition, our re-analysis suggests a significant change in BMI. The original paper also reported significant changes in specific gut bacterial families (butyrate-producing bacteria, p = 0.037; Lactobacillus species, p = 0.006). Physical activity levels remained consistently low. Full article

Physical inactivity, which currently dominates the lifestyles of most people, is linked to chronic metabolic diseases such as obesity, type 2 diabetes (T2D), hypertension, metabolic syndrome, and cardiovascular disease, all of which share insulin resistance as a common pathogenic mechanism. Both epidemiological and experimental intervention studies have consistently shown that physical activity and exercise can reduce the incidence of these diseases and significantly improve their clinical outcomes, resulting in enhanced quality of life and well-being. This approach includes various forms of aerobic and anaerobic/resistance training, either individually or in combination, leading to reduced insulin resistance and visceral fat, regardless of the weight loss achieved through diet. It also lowers inflammatory responses and oxidative stress, a harmful mechanism that leads to cellular damage, and positively impacts immunological regulation. Regarding timing, physical activity/exercise appears to produce better outcomes for metabolic control, particularly in individuals with T2D, when performed after dinner compared to other times of the day. In addition to organized physical activity/exercise sessions, practices such as interrupting prolonged sitting with frequent breaks every 30 min that involve muscular contractions and increased energy expenditure may also benefit metabolic health. Minimizing physical inactivity, prolonged sitting at work or during leisure time, can decrease the frequency of metabolic illness, enhance health and quality of life, and avert premature death. However, intense exercise may not always be the most beneficial option for health, and the relationship between adverse events and the intensity of physical activity or exercise resembles a U-shaped or J-shaped curve. Physical activity/exercise should be performed at a suitable intensity that aligns with personal capability. In this primarily clinically focused review, we discuss the effects of insulin on target tissues, the significance of insulin sensitivity in metabolic regulation, how physical inactivity contributes to insulin resistance, the different types of exercise and their impact on insulin effectiveness, and the importance of physical activity and exercise in managing metabolic diseases. Full article

Overweight and obesity are major public health concerns among children and adolescents worldwide. The most prevalent form is exogenous–constitutional obesity, which is driven by a sedentary lifestyle and an unhealthy diet in which caloric intake exceeds energy expenditure. Beyond their association with chronic disease, these factors are closely linked to deficits in cognitive development and executive functions essential for learning (including working memory, sustained attention, planning, behavioral self-regulation, and cognitive flexibility). Oxidative stress (OS), characterized by the accumulation of reactive oxygen species (ROS) in cells and extracellular fluids, is a significant potential mediator in childhood obesity and an important contributor to its comorbidities. The antioxidant defense system (AOD)’s activity largely depends on levels of trace element cofactors, which determine the body’s resistance to adverse environmental factors (the “maladaptation phenomenon”). OS and trace element deficiencies contribute to the development of morphological changes in the brain, thus serving as a critical connecting link between childhood obesity and cognitive impairment. Non-pharmacological interventions are the most accessible and effective approach for prevention and treatment. Bioactive compounds derived from food and natural plants, classified as antioxidants and phytopreparations, may represent a promising complementary approach. These compounds are most effective when used in combination with sustained lifestyle modifications in children. Research in this area can help define future directions for study and develop targeted intervention strategies in the pediatric population. The aim of this review is to examine the relationship between OS, antioxidant cofactor micronutrients, and cognitive outcomes in childhood obesity and to explore mechanisms, evidence, and therapeutic opportunities. Full article

Background/Objectives: Low physical activity (PA) is becoming an increasingly serious health problem among overweight school-age children. This study aimed to evaluate the influence of elevated PA during school hours on the nutritional behavior and fat mass of overweight and obese children. Methods: The study involved 11-year-old children (n = 148) who were overweight and obese. In the control group, children received physical education lessons in the standard dimension (4 h a week) while the intervention group received 10 h. Body mass index (BMI), fat mass (FM), and nutritional behavior were analyzed. Results: Compared to baseline, at the end of the intervention, the proportion of obese children increased in the control group and decreased in the intervention group. Regarding nutritional behavior, low consumption of vegetables, fruits, whole grains, poultry, and fish was observed. After a 12-month period, the intervention group showed a slight decrease in the consumption of fruits, vegetables, cold cuts, fried dishes, sweets, and fast food, and an increase in the consumption of white bread, whole-grain bread, poultry, red meat, and dairy products (milk, yogurt). In the control group, children exhibited an average increase in the consumption of fruits, vegetables, poultry, red meat, sweets, and fast food Conclusions: Although extended physical activity during school hours was correlated with a decrease in the number of obese children, the observational nature of the study precludes the drawing of definitive conclusions. The intervention may have contributed to an increase in energy expenditure, which could account for the improvements in BMI and FM. Nevertheless, the impact on nutritional behavior was limited. Full article