Search Results (40)

This study presents an automatic PID control strategy for Tapping-Mode Atomic Force Microscopy (TM-AFM) that addresses the impacts of energy dissipation on tip–sample interactions. Our methodology integrates energy analysis to quantify the critical relationship between energy loss and phase lag dynamics in tapping mode. Additionally, systematic decomposition of interaction force is performed to enable the reconstruction of system transfer functions. The study in this work examines the fluctuations of PID gains during critical oscillations. A SIMULINK-based virtual TM-AFM is developed to simulate practical measurement conditions, based on which a lookup table for PID gains across various phase lags is generated. The efficacy of the proposed algorithm is experimentally validated through the experiments of a calibration nanogrid and two kinds of coated silicon samples, demonstrating the improved tracking accuracy and the improvement of surface height of 5.4% compared to regular control scheme. Full article

Serum amyloid A (SAA) is a small protein consisting of 104 residues and, under physiological conditions, exists mainly in hexameric form. It belongs to the positive acute-phase proteins, which means that its plasma concentration increases rapidly in response to injury, inflammation, and infection. The accumulation of SAA molecules promotes the formation of amyloid aggregates, which deposit extracellularly in many organs, causing their dysfunction. In our previous work, we successfully designed a peptidomimetic that inhibited the aggregation of amyloidogenic SAA fragments. In the present paper, we show how the same inhibitor, named saa3Dip, affects the oligomerization and aggregation processes of MetSAA1.1 protein. The thioflavin T assay showed that saa3Dip inhibited its fibrillization. The measurement of the internal fluorophore fluorescence (Trp) showed differences that occurred in the tertiary structure of MetSAA1.1 in the presence of the inhibitor, which was also confirmed by CD spectra in the aromatic range. FTIR results suggested that saa3Dip could stabilize some fragments of the native structure of MetSAA1.1, which was confirmed by determining the melting temperature (Tm) of the protein–inhibitor complex. AFM images demonstrated that the presence of saa3Dip prevented the formation of large SAA aggregates. Our results suggest that saa3Dip stabilizes the native conformation of MetSAA1.1. Full article

In this work, the structure of silica thin films synthesized with three different SiO₂ precursors and obtained by the sol–gel method and dip coating technique was studied. Additionally, the influence of Ag addition on the obtained silica sols and then gel structure was investigated. Silica coatings show antireflective properties and high thermal resistance, as well as hydrophobic or hydrophilic properties. Three different silica precursors, TEOS (tetraethylorthosilicate), DDS (dimethyldietoxysilane) and Aerosil^TM, were selected for the synthesis. DDS added to silica sol act as a pore size modifier, while Ag atoms are known for their antibacterial activity. Coatings were deposited on two different substrates: steel and titanium, dried and annealed at 500 °C in air (steel substrate) and in argon (titanium substrate). For all synthesized films, IR (infrared) spectroscopic studies were performed together with GID and XRD (Grazing Incidence Diffraction, X-ray Diffraction) measurements. The topography and morphology of the surface were traced by SEM and AFM microscopic methods, providing information on the samples’ roughness, particle sizes and thickness of the particular layers. The wetting angle values were also measured. GID and XRD measurements pointed to the distinct contribution of an amorphous phase in the samples, allowing us to recognize the crystalline phases and calculate the silver crystallite sizes. The FTIR spectra gave information on the first coordination sphere of the studied samples. Full article

The tapping-mode atomic force microscope (TM-AFM) is widely used today; however, improper matching between the operating medium and the sampling time may lead to inaccurate measurement results. The relationship between the damping coefficient and the steady state of the TM-AFM microcantilever is investigated in this paper using multiple stability theory. Firstly, the effects of changes in dimensionless linear damping coefficients and dimensionless piezoelectric film damping coefficients on the motion stability of the system are examined using bifurcation diagrams, phase trajectories, and domains of attraction. Subsequently, the degrees of effect of the two damping coefficients on the stability of the system are compared. Finally, the bi-parametric bifurcation characteristics of the system under a specific number of iterative cycles are investigated using the bi-parametric bifurcation diagram in conjunction with the actual working conditions, and the boundary conditions for the transition of the system’s motion from an unstable state to a stable state are obtained. The results of the study show that to ensure the accuracy and reliability of the individual measurement data in 500 iteration cycles, the dimensionless linear damping coefficient must be greater than 0.01014. Our results will provide valuable references for TM-AFM measurement media selection, improving TM-AFM imaging quality, measurement accuracy and maneuverability, and TM-AFM troubleshooting. Full article

A versatile and robust procedure is developed that allows the identification of individual target molecules using antibodies bound to a DeepTip^TM functionalized atomic-force microscopy probe. The model system used for the validation of this process consists of a biotinylated anti-lactate dehydrogenase antibody immobilized on a streptavidin-decorated AFM probe. Lactate dehydrogenase (LDH) is employed as target molecule and covalently immobilized on functionalized MicroDeck^TM substrates. The interaction between sensor and target molecules is explored by recording force–displacement (F–z) curves with an atomic-force microscope. F–z curves that correspond to the genuine sensor–target molecule interaction are identified based on the following three criteria: (i) number of peaks, (ii) value of the adhesion force, and (iii) presence or absence of the elastomeric trait. The application of these criteria leads to establishing seven groups, ranging from no interaction to multiple sensor–target molecule interactions, for which force–displacement curves are classified. The possibility of recording consistently single-molecule interaction events between an antibody and its specific antigen, in combination with the high proportion of successful interaction events obtained, increases remarkably the possibilities offered by affinity atomic-force microscopy for the characterization of biological and biomimetic systems from the molecular to the tissue scales. Full article

(1) Background: the current study investigated three nanohybrid composites: two commercial products ClearfilMajesty^TM (CM) and Harmonize^TM (HU), compared with an experimental product PS2. (2) Methods: Two sample types were molded using Teflon dies. The first sample type was represented by standard discs (20 mm diameter and 2 mm thickness) (n = 60, 20/each material), used for surface conditioning investigation, specifically roughness monitoring and color stability analysis using AFM and the CIELab test, respectively. The second sample type was a standard cylindrical specimen (4 mm diameter and 6 mm height) for compression testing (n = 60, 20/each material). After complete polymerization, the samples were ground with sandpaper and further polished. The filler size and distribution in the polymer matrix were investigated with SEM. Data were statistically analyzed using the Anova Test followed by Tukey’s post hoc test on the Origin Lab 2019 software produced by OriginLab Corporation, Northampton, MA, USA. (3) Results: A mono-disperse system was identified in HU samples, while CM and PS2 revealed both nano- and microfiller particles. The samples’ observation after immersion in coffee and tea indicated that a lower roughness combined with optimal filler lamination within the polymer matrix assured the best color preservation. The compression strength was lower for the HU sample, while higher values were obtained for the complex filler systems within CM and PS2. (4) Conclusions: the behavior of the investigated nanohybrid composites strongly depends on the microstructural features. Full article

Commercially available formulations of the popular conductive polymer, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) are aqueous dispersions that require the addition of secondary dopants such as dimethyl sulphoxide (DMSO) or ethylene glycol (EG) for fabricated films to have the desired levels of conductivity. Clevios^TM F HC Solar, a formulation of PEDOT:PSS produced by Heraeus, GmbH, achieves over 500 S/cm without these secondary dopants. This work studies whether secondary dopants such as DMSO have any additional effect on this type of PEDOT:PSS. The temperature dependencies of the conductivity of F HC Solar spin-coated thin films measured using a four-probe method seem to exhibit different charge transport properties compared with secondary doped PH1000. Observations made using atomic force microscopy (AFM) show that different concentrations of DMSO affect the orientation of the PEDOT domains in the thin film. These morphological changes cause room temperature conductivity to reduce from 640 S/cm in pristine films to as low as 555 S/cm after adding 7 wt% of DMSO along the film. Such tuning may prove useful in future applications of PEDOT:PSS, such as nanoprobes, transistors and hybrid solar cells. Full article

Alterations in the dynamical properties of an atomic force microscope microcantilever beam system in tapping mode can appreciably impact its measurement precision. Understanding the influence mechanism of dynamic parameter changes on the system’s motion characteristics is vital to improve the accuracy of the atomic force microscope in tapping mode (AFM-TM). In this study, we categorize the mathematical model of the AFM-TM microcantilever beam system into systems 1 and 2 based on actual working conditions. Then, we analyze the alterations in the dynamic properties of both systems due to external excitation variations using bifurcation diagrams, phase trajectories, Lyapunov indices, and attraction domains. The numerical simulation results show that when the dimensionless external excitation g < 0.183, the motion state of system 2 is period 1. When g < 0.9, the motion state of system 1 is period 1 motion. Finally, we develop the equivalent circuit model of the AFM-TM microcantilever beam and perform related software simulations, along with practical circuit experiments. Our experimental results indicate that the constructed equivalent circuit can effectively analyze the dynamic characteristics of the AFM-TM microcantilever beam system in the presence of complex external environmental factors. It is observed that the practical circuit simulation attenuates high-frequency signals, resulting in a 31.4% reduction in excitation amplitude compared to numerical simulation results. This provides an essential theoretical foundation for selecting external excitation parameters for AFM-TM cantilever beams and offers a novel method for analyzing the dynamics of micro- and nanomechanical systems, as well as other nonlinear systems. Full article

Currently, two-dimensional (2D) materials with intrinsic antiferromagnetism have stimulated research interest due to their insensitivity to external magnetic fields and absence of stray fields. Here, we predict a family of stable transition metal (TM) borides, TMB₁₂ (TM = V, Cr, Mn, Fe) monolayers, by combining TM atoms and B₁₂ icosahedra based on first-principles calculations. Our results show that the four TMB₁₂ monolayers have stable antiferromagnetic (AFM) ground states with large magnetic anisotropic energy. Among them, three TMB₁₂ (TM=V, Cr, Mn) monolayers display an in-plane easy magnetization axis, while the FeB₁₂ monolayer has an out-of-plane easy magnetization axis. Among them, the CrB₁₂ and the FeB₁₂ monolayers are AFM semiconductors with band gaps of 0.13 eV and 0.35 eV, respectively. In particular, the AFM FeB₁₂ monolayer is a spin-polarized AFM material with a Néel temperature of 125 K. Moreover, the electronic and magnetic properties of the CrB₁₂ and the FeB₁₂ monolayers can be modulated by imposing external biaxial strains. Our findings show that the TMB₁₂ monolayers are candidates for designing 2D AFM materials, with potential applications in electronic devices. Full article

The topography and composition of dental implant surfaces directly impact mesenchymal cell adhesion, proliferation, and differentiation, crucial aspects of achieving osseointegration. However, cell adhesion to biomaterials is considered a key step that drives cell proliferation and differentiation. The aim of this study was to characterize characterize the topography and composition of commercial titanium dental implants manufactured with different surface treatments (two sandblasted/acid-etched (SLA) (INNO Implants, Busan, Republic of Korea; BioHorizons^TM, Oceanside, CA, USA) and two calcium phosphate (CaP) treated (Biounite^®, Berazategui, Argentina; Zimmer Biomet, Inc., Warsaw, IN, USA)) and to investigate their influence on the process of cell adhesion in vitro. A smooth surface implant (Zimmer Biomet, Inc.) was used as a control. For that, high-resolution methodologies such as scanning electron microscopy (SEM), X-ray dispersive spectroscopy (EDX), laser scanning confocal microscopy (LSCM), and atomic force microscopy (AFM) were employed. Protein adsorption and retromolar gingival mesenchymal stem cells (GMSCs) adhesion to the implant surfaces were evaluated after 48 h. The adherent cells were examined by SEM and LSCM for morphologic and quantitative analyses. ANOVA and Tukey tests (α = 0.05) were employed to determine statistical significance. SEM revealed that INNO, BioHorizons^TM, and Zimmer implants have an irregular surface, whereas Biounite^® has a regular topography consisting of an ordered pattern. EDX confirmed a calcium and phosphate layer on the Biounite^® and Zimmer surfaces, and AFM exhibited different roughness parameters. Protein adsorption and cell adhesion were detected on all the implant surfaces studied. However, the Biounite^® implant with CaP and regular topography showed the highest protein adsorption capacity and density of adherent GMSCs. Although the Zimmer implant also had a CaP treatment, protein and cell adhesion levels were lower than those observed with Biounite^®. Our findings indicated that the surface regularity of the implants is a more determinant factor in the cell adhesion process than the CaP treatment. A regular, nanostructured, hydrophilic, and moderately rough topography generates a higher protein adsorption capacity and thus promotes more efficient cell adhesion. Full article

This study explores the effects of four independent variables—the nanoclay weight percentage, sliding velocity, load, and sliding distance—on the wear rate and frictional force of nanoclay-filled FormuLITE^TM amine-cured bio-based epoxy composites. An experimental design based on the Taguchi method revealed diverging optimal conditions for minimizing the wear and frictional force. These observations were further validated using a Back-propagation Artificial Neural Network (BPANN) model, demonstrating its proficiency in predicting complex system behavior. Material characterization, conducted through Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray Spectroscopy (EDS), illustrated the homogeneous distribution of the nanoclay within the Formulite^TM matrix, which is crucial for enhancing the load transfer and stress distribution. Atomic Force Microscopy (AFM) analysis indicated that the incorporation of nanoclay increases the surface roughness and peak height, which are important determinants of the material performance. However, an increase in the nanoclay percentage decreased these attributes, suggesting an interaction saturation point. Due to their augmented mechanical properties, the present study underscores the potential of amine-cured bio-based epoxy systems in diverse applications, such as automotive, aerospace, and biomedical engineering. Full article

Brackets are metallic dental devices that are very often associated with acidic soft drinks such as cola and energy drinks. Acid erosion may affect the bonding between brackets and the enamel surface. The purpose of this study was to investigate the characteristics of brackets’ adhesion, in the presence of two different commercially available drinks. Sixty human teeth were divided into six groups and bonded with either resin-modified glass ionomer (RMGIC) or resin composite (CR). A shared bond test (SBS) was evaluated by comparing two control groups with four other categories, in which teeth were immersed in either Coca-Cola^TM or Red Bull^TM energy drink. The debonding between the bracket and enamel was evaluated by SEM. The morphological aspect correlated with SBS results showed the best results for the samples exposed to artificial saliva. The best adhesion resistance to the acid erosion environment was observed in the group of teeth immersed in Red Bull^TM and with brackets bonded with RMGIC. The debonded structures were also exposed to Coca-Cola^TM and Red Bull^TM to assess, by atomic force microscopy investigation (AFM), the erosive effect on the enamel surface after debonding and after polishing restoration. The results showed a significant increase in surface roughness due to acid erosion. Polishing restoration of the enamel surface significantly reduced the surface roughness that resulted after debonding, and inhibited acid erosion. The roughness values obtained from polished samples after exposure to Coca-Cola^TM and Red Bull^TM were significantly lower in that case than for the debonded structures. Statistical results evaluating roughness showed that Red Bull^TM has a more erosive effect than Coca-Cola™. This result is supported by the large contact surface that resulted after debonding. In conclusion, the prolonged exposure of the brackets to acidic drinks affected the bonding strength due to erosion propagation into both the enamel–adhesive interface and the bonding layer. The best resistance to acid erosion was obtained by RMGIC. Full article

Polyphthalocyanines (PPCs) are a new and promising class of two dimensional materials offering versatile avenues for next generation electronic devices. For organic spintronic devices, PPCs can be engineered to tailor the electric and magnetic properties. In this work, we investigate PPC’s monolayers with embedded transition metal atoms (TM = Fe, Ni, Cu), utilizing first principle calculations based on spin-polarized generalized gradient approximation (SGGA). PPC sheets with central TM atoms are simulated for the dispersion curves, electronic density of states (DOS), and projected density of states (PDOS) using quantum atomistic toolkit (Quantum ATK) software. According to simulations, the FePPC supercell with four magnetic moments of Fe, aligned in a parallel ferromagnetic (FM) configuration, show the conductive FM state, while in the case of the anti-parallel antiferromagnetic (AFM) order of the magnetic moments, the material exhibits semiconducting non-magnetic behavior. FM-ordered NiPPC displays a metallic state, which is partly suppressed for AFM-ordered NiPPC. In contrast, non-magnetic CuPPC is found to be the best conductor due to its larger PDOS at the Fermi level among all considered systems. Full article

Thermogelling amphiphilic block copolymers have been widely investigated in the development of pharmaceutical drug carriers. In particular, thermosensitive gels based on poloxamer 407 (P407) have great potential for periodontal disease treatment, thanks to their ability to be liquid at room temperature and become viscous gels at body temperature. However, some problems, related to short in situ residence time, reduce their feasible clinical use. Thus, in order to improve the effective applicability of these materials, we studied how P407 thermogels are affected by the pH and by the inclusion of different hydrophilic polymers, used as excipients for increasing the gel stiffness. For this scope, a complete chemical-physical characterization of the synthesized gels is provided, in terms of determination of sol-gel transition temperature, viscosity and erosion degree. The data are correlated according to a statistical multivariate approach based on Principal Component Analysis and their mucoadhesion properties are also tested by Tapping mode-Atomic Force Microscopy (TM-AFM) imaging. Finally, we studied how the different P407 formulations are able to influence the release pathway of two antibacterial drugs (i.e., chlorhexidine digluconate and doxycycline hyclate) largely used in oral diseases. Full article

Elevated intraocular pressure (IOP) has been considered to be the major risk factor for glaucoma. The trabecular meshwork (TM), serving as the primary outflow pathway of aqueous humor (AH), has an essential role in controlling IOP. However, the mechanism of the interaction between ocular hypertension (OHT) and TM has not been completely understood. This research intended to investigate the effect of OHT on cell stiffness, cytoskeletal structure, and vimentin expression of TM cells in rats. Polystyrene microspheres were injected into the left eye of Sprague–Dawley (SD) rats to establish the OHT animal models. TM cells were extracted from the rat eyes with 2-week duration of OHT using the trypsin digestion method. The effect of OHT on the cytoskeletal structure and expression level of vimentin in TM cells were evaluated by immunofluorescence and western blot analyses, respectively. An atomic force microscope (AFM) was used to measure the cell elastic modulus. On the eighth day after microsphere injection, the IOP of experimental eyes increased to 24.25 ± 2.21 mmHg, and the IOP was maintained above 24 mmHg until the end of the experiment. TM cells extracted from the OHT group showed cytoskeleton rearrangement and the formation of cross-linked actin networks (CLANs). Compared to the control group, the cell stiffness in the OHT group was significantly increased. Moreover, the expression level of vimentin was obviously decreased in TM cells of the OHT group. The results demonstrate that OHT induces the rearrangement of the cytoskeleton, increase of cell stiffness, and downregulation of vimentin expression in TM cells. Full article