Search Results (199)

The growing demand for sustainable plant-based dairy alternatives has spurred interest in valorizing agro-industrial byproducts like hazelnut cake, a protein-rich byproduct of oil extraction. This study developed formulations for vegan ice cream using unfermented (HIC) and Aspergillus oryzae-fermented hazelnut cake (FHIC), comparing their physicochemical, functional, and sensory properties to conventional dairy ice cream (DIC). Solid-state fermentation (72 h, 30 °C) enhanced the cake’s bioactive properties, and ice creams were characterized for composition, texture, rheology, melting behavior, antioxidant activity, and enzyme inhibition pre- and post-in vitro digestion. The results indicate that FHIC had higher protein content (64.64% vs. 58.02% in HIC) and unique volatiles (e.g., benzaldehyde and 3-methyl-1-butanol). While DIC exhibited superior overrun (15.39% vs. 4.01–7.00% in vegan samples) and slower melting, FHIC demonstrated significantly higher post-digestion antioxidant activity (4.73 μmol TE/g DPPH vs. 1.44 in DIC) and angiotensin-converting enzyme (ACE) inhibition (4.85–7.42%). Sensory evaluation ranked DIC highest for overall acceptability, with FHIC perceived as polarizing due to pronounced flavors. Despite textural challenges, HIC and FHIC offered nutritional advantages, including 18–30% lower calories and enhanced bioactive compounds. This study highlights fermentation as a viable strategy to upcycle hazelnut byproducts into functional vegan ice creams, although the optimization of texture and flavor is needed for broader consumer acceptance. Full article

Açaí (Euterpe oleracea) seeds account for up to 95% of the fruit’s weight and are commonly discarded during pulp processing. Roasted açaí seed extract (RASE) has recently emerged as a caffeine-free coffee substitute, although its composition and functionality remain underexplored. This study characterized commercial açaí seed powder and evaluated the effect of temperature on the recovery of total phenolic content (TPC) in the aqueous extract using a Central Composite Rotatable Design (CCRD). An intermediate extraction condition (6.0 ± 0.5 g 100 mL⁻¹ at 100 °C) was selected, resulting in 21.78 mg GAE/g TPC, 36.23 mg QE/g total flavonoids, and notable antioxidant capacity (FRAP: 183.33 µmol TE/g; DPPH: 23.06 mg TE/g; ABTS: 51.63 mg TE/g; ORAC: 31.46 µmol TE/g). Proton Nuclear Magnetic Resonance (¹H NMR) analysis suggested the presence of amino acids, carbohydrates, and organic acids. During in vitro digestion, TPC decreased from 54.31 to 17.48 mg GAE 100 mL⁻¹ when RASE was combined with goat milk. However, higher bioaccessibility was observed with skimmed (33%) and semi-skimmed (35%) cow milk. These findings highlight RASE as a phenolic-rich, antioxidant beverage with functional stability when prepared with boiling water. This is the first study to report the phytochemical profile of RASE and its interactions with different milk types, supporting its potential as a coffee alternative. Full article

In recent years, most of the populations of the world have been using herbal materials for their strong antimicrobial properties and major health benefits. The objective of this study was to evaluate the phenolic profile, as well as the antioxidant and antimicrobial activities, of a dietary supplement composed of extracts from hibiscus (Hibiscus sabdariffa L.) calyces and lemon verbena (Lippia citriodora) leaves (Metabolaid^®, Patent P201731147) mixed at a weight ratio of 35:65 (w/w), respectively. The bioactive components of the methanolic extract were analyzed by UHPLC-ESI-MS/MS. The antioxidant activity was evaluated using spectrophotometric methods, while the antimicrobial activity was assessed through the microdilution method against selected Gram-negative and Gram-positive bacteria. The total phenols content resulted in being 256.10 ± 2.26 mg GAE/g f.w., the flavonoid content was 48.90 ± 2.95 mg CE/g f.w., flavonols were 60.17 ± 7.68 mg QE/g f.w., and anthocyanins were 3.78 ± 0.17 mg C3GE/g f.w. The FRAP value, observed in the natural mix additive, was 1.25 ± 0.03 mg Fe²⁺/g f.w., while the ORAC showed the value of 1893.77 ± 30.39 µmol TE/g f.w. and the DPPH was 23.33 ± 4.12 µg/mL. We found eight phenolic acids, seven flavonols, five anthocyanins, and nine other phenolic compounds. The extract showed a minimum inhibitory concentration (MIC) of 12.5 mg/mL against E. coli, E. aerogenes, and E. faecalis and of 25 mg/mL against S. enterica ser. Typhimurium and S. aureus and a minimum bactericidal concentration (MBC) of 25 mg/mL against E. coli, E. aerogenes, and E. faecalis and of 50 mg/mL against S. enterica ser. Typhimurium and S. aureus. In conclusion, our findings demonstrate that Metabolaid^® is a rich source of bioactive compounds and provides beneficial effects against oxidative stress and pathogenic bacteria, supporting its nutraceutical potential. Full article

Carob pulp powder (CPP), a by-product of Ceratonia siliqua L., is rich in bioactive compounds with potential for functional foods. This study evaluated how genetic variability among cultivars affects the nutritional composition and functional quality of CPP. Nineteen cultivars from Majorca (13), Ibiza (4), and two open-flowering selections were grown under identical conditions in Majorca, Spain. CPP samples showed high dietary fibre (22.6–47.4 g/100 g dry matter), total sugars (22.5–62.5 g/100 g dm), and antioxidant activity (11.1–78.4 µmol TE/g dm, CUPRAC method). Significant differences among cultivars were observed in protein and fructose content, fatty acid profiles, antioxidant activity, colour, acidity, and functional properties like water- and oil-holding capacities. Principal component analysis distinguished the Ibiza cultivars by higher soluble solids, fructose, and stearic acid content but lower antioxidant activity. Open-flowering selections exhibited the highest antioxidant and water retention capacities. These results confirm that genetic origin strongly influences CPP’s nutritional and functional traits, endorsing its use as a natural, health-promoting ingredient for functional food development. Full article

Background/Objectives: Agave amica (Medik.) Thiede & Govaerts (Asparagaceae family) is an ornamental bulbous species, widely used for its fragrance but less studied as a medicinal species. This study is aimed at assessing the phenolic profile and selected biological properties of ethanolic extracts obtained from the aerial parts and bulbs of A. amica cultivated in Romania. Methods: The phenolic composition was characterized by spectrophotometric methods and LC/MS analysis. The antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity) and FRAP (Ferric reducing antioxidant power) tests, while the in vitro antimicrobial capacity was investigated by the agar-well diffusion, the broth microdilution, and the antibiofilm assays. Cytotoxicity was tested on a colorectal adenocarcinoma cell line (DLD-1) by a CCK-8 assay. Results: Both ethanolic extracts showed important polyphenol content and caffeic acid as their main compound. Significantly higher amounts of total polyphenols (44.25 ± 1.08 mg/g), tannins (12.55 ± 0.34 mg/g), flavonoids (9.20 ± 0.19 mg/g), caffeic acid derivatives (19.95 ± 0.05 mg/g), and also antioxidant activity (IC₅₀ = 0.82 ± 0.02 mg/mL, and 79.75 ± 1.80 µM TE/g, respectively) were found for the aerial parts extract compared to the bulbs one (p < 0.001). Notable anti-Candida albicans activity and moderate efficacy against Listeria monocytogenes and Staphylococcus aureus were displayed by both extracts against planktonic cells and biofilm. A dose-dependent cytotoxicity towards the colorectal adenocarcinoma cell line was recorded as well. Conclusions: This study brings novelty to the scientific literature by characterizing the phenolic profile and in vitro antimicrobial, antibiofilm, antioxidant, and antiproliferative activities of the ethanolic extracts obtained from A. amica, thus highlighting this herbal species’s medicinal potential. Full article

In this study, the effects of different heat sink designs on the cold side of the modules in a thermoelectric generator (TEG) system placed between the compressor and the intercooler of a turbocharged tractor on the system performance were numerically analyzed. In the current literature, heat sinks used in TEG modules generally consist of plate fins. In this study, by using perforated and slotted fins, the thermal boundary layer behaviors were changed and there was an attempt to increase the heat transfer from the cold surface compared to plate fins. Thus, the performance of the TEG system was also increased. When looking at the literature, it is seen that there are studies which aim to increase the performance of TEG modules by changing the dimensions of p and n type semiconductors. However, there is no study aiming to increase the performance of TEG modules by making changes on the plate fins of the heat sinks used in these modules and thus increasing the heat transfer amount. In this respect, this study offers important results for the literature. According to the numerical analysis results, the total TEG output power, output voltage, and thermal efficiency obtained for S0.5H15 were 6.2%, about 3%, and about 5% higher than those for PF, respectively. In addition, the pressure drop values obtained for different heat sinks, except for aluminum foam, were approximately close to each other. In cases with TEG systems where different heat sinks were used, the intercooler inlet air temperatures decreased by approximately 3.4–3.5% compared to the case without the TEG system. This indicates that the use of TEG will positively affect the improvement in engine efficiency. Full article

In this study, borophene and nickel phthalocyanine (NiPc): borophene nanocomposites were prepared using the sonication method. The NiPc: borophene nanocomposite was uniformly obtained as a 10–80 nm-sized spherically shaped particle. Electrical conductivities (s) were measured as 3 × 10⁻¹³ Scm⁻¹ and 9.5 × 10⁻⁹ Scm⁻¹ for NiPc and the NiPc: borophene nanocomposite, respectively. The SEM image showed that borophene was homogeneously distributed in the NiPc matrix and increased the charge transport pathways. This is the main reason for a 10⁶-fold increase in electrical conductivity. An indium tin oxide (ITO)/NiPc: borophene nanocomposite-based thermoelectric generator (TEG) was prepared and characterized. The Seebeck coefficients (S) were calculated to be 5 μVK⁻¹ and 30 μVK⁻¹ for NiPc and the NiPc: borophene nanocomposite, respectively. A positive Seebeck coefficient value for the NiPc: borophene showed the p-type nature of the nanocomposite. The power factors (PF = sS2) were calculated as 7.5 × 10⁻¹⁶ μW m⁻¹ K⁻² and 8.6 × 10⁻¹⁰ μW m⁻¹ K⁻² for NiPc and the NiPc: borophene nanocomposite, respectively. Compositing NiPc with borophene increased the power factor by ~10⁶-fold. It has been concluded that the electrical conductivity and Seebeck coefficient of the NiPc: borophene material increases due to energy band convergence because of combining p-type NiPc with p-type borophene. Therefore, the NiPc: borophene nanocomposite is a promising material for TEG. Full article

Microalgae represent promising biotechnological platforms for bioactive compound production with pharmaceutical applications. This study investigated the phytochemical composition and biological activities of lipid extracts from three Chlorella species to evaluate their potential as antioxidant and antidiabetic sources. Lipid extraction using chloroform–methanol (2:1) followed by GC–MS analysis revealed distinct compound distributions: 29 compounds in C. ellipsoidea, 33 in C. sorokiniana, and 19 in C. vulgaris. Major bioactive compounds included 2-hexanol, 1,3,6-heptatriene, 4-(2,3-dimethyl-2-cyclopenten-1-yl)-4-methylpentanal, n-hexadecanoic acid, and octadecanoic acid. Biological activity screening encompassed antioxidant assessment through DPPH• and •NO radical scavenging assays and FRAP analysis, while antidiabetic potential was evaluated using α-glucosidase and α-amylase inhibition assays. C. sorokiniana exhibited superior bioactivity with the highest antioxidant capacity (DPPH• IC₅₀ = 329.03 ± 4.30 µg/mL; •NO IC₅₀ = 435.53 ± 10.20 µg/mL; FRAP = 94.74 ± 5.72 mg TE/g) and strongest enzyme inhibition (α-glucosidase IC₅₀ = 752.75 ± 57.95 µg/mL; α-amylase IC₅₀ = 3458.50 ± 104.01 µg/mL). This is the first report on C. sorokiniana strain KU.B2′s biological properties and phytochemical profile. These findings establish C. sorokiniana as a valuable biotechnological platform for pharmaceutical bioactive compound development. Full article

This study develops a value-added processing technique for grade-out cape gooseberry (Physalis peruviana Linn.) by applying mild hydrostatic osmotic pretreatment combined with rotary tray drying. Fruits classified as grade-out, often discarded due to aesthetic flaws, were subjected to osmotic treatment at 0.5 bar for 12 h using a sucrose solution enhanced with citric acid and glycerin. Pretreatment significantly elevated water loss (52.61%) and solid gain (18.12%), reducing moisture content prior to drying. Rotary tray drying was conducted at temperatures of 50, 60, and 70 °C. Drying at 60 °C achieved the ideal balance between efficiency and product quality. Samples pretreated and dried at 60 °C exhibited a 35% reduction in drying time while preserving superior color (ΔE = 13.54 ± 1.81), vitamin C (71.76 ± 2.57 mg/100 g dry matter, DM), total phenolic content (202.9 ± 10.91 mg GAE/100 g DM), and antioxidant activity (ABTS = 95.87 ± 3.41 µmol TE/g DM; DPPH = 89.97 ± 1.27 µmol TE/g DM). A production trial was conducted using 1500 kg of raw material from the Mae Hae Royal Project Development Center in Chiang Mai, Thailand. This process yielded 220 kg of high-quality dried fruit at an overall cost of USD 6.93 per kg. Local farmers successfully applied this technique, demonstrating its potential to enhance livelihoods, avoid postharvest losses, and valorize low-quality produce in line with Sustainable Development Goal 12. This supports the Royal Project Foundation’s vision for sustainable agriculture. Full article

Hypericum perforatum L. (H.P.), a plant renowned for its wound-healing properties, was investigated for antioxidant/antimicrobial efficacy, toxicological safety, and in vivo wound-healing effects in this research to develop and characterize novel nanoemulsion hydrogel (NG) formulations. NG were prepared via emulsion diffusion–solvent evaporation and polymer hydration using Cremophor RH40 and Ultrez 21/30. A D-optimal design optimized oil/surfactant ratios, considering particle size, PDI, and drug loading. Antioxidant activity was tested via DPPH, ABTS⁺, and FRAP. Toxicological assessment followed HET-CAM (ICH-endorsed) and ICCVAM guidelines. The optimized NG-2 (NE-HPM-10 + U30 0.5%) demonstrated stable and pseudoplastic flow, with a particle size of 174.8 nm, PDI of 0.274, zeta potential of −23.3 mV, and 99.83% drug loading. Release followed the Korsmeyer–Peppas model. H.P. macerates/NEs showed potent antioxidant activity (DPPH IC₅₀: 28.4 µg/mL; FRAP: 1.8 mmol, Fe²⁺/g: 0.3703 ± 0.041 mM TE/g). Antimicrobial effects against methicillin-resistant S. aureus (MIC: 12.5 µg/mL) and E. coli (MIC: 25 µg/mL) were significant. Stability studies showed no degradation. HET-CAM tests confirmed biocompatibility. Histopathology revealed accelerated re-epithelialization/collagen synthesis, with upregulated TGF-β1. The NG-2 formulation demonstrated robust antioxidant, antimicrobial, and wound-healing efficacy. Enhanced antibacterial activity and biocompatibility highlight its therapeutic potential. Clinical/pathological evaluations validated tissue regeneration without adverse effects, positioning H.P.-based nanoemulsions as promising for advanced wound care. Full article

Background: Stachys alpina is a medicinal plant from the Lamiaceae family whose biological potential remains poorly explored. Methods: The aim of this study was to comprehensively assess the pol-yphenol profile, macro- and microelement composition, and the antioxidant, an-ti-diabetic, and anti-obesity activities of various plant organs (leaves, flowers, stems, and roots). Results: The leaves and flowers exhibited the highest concentration of phenolic compounds, while anthocyanins were detected exclusively in the flowers (215.05 mg/100 g dry matter (dm)) and constituted 3% of the total polyphenols. Verbas-coside and chlorogenic acid were the most abundant polyphenols, reaching 4618.88 and 3277.83 mg/ 100 g dm in the leaves. The highest ABTS and FRAP scavenging activity was observed in leaves (19.30 and 7.62 mmol TE/g dm, respectively). Principal component analysis demonstrated a strong correlation between polyphenol content and antioxidant activity (ABTS-r= 0.87 and FRAP-r = 0.90), which was further confirmed by Pearson’s correlation coefficients. The study also highlighted the significant impact of mineral composition on biological activity—calcium and magnesium dominated in stems (10,100 and 3900 mg/kg) and in roots (9200 and 3100 mg/kg), supporting the functioning of an-tioxidant enzymes, while zinc and manganese in leaves (89.43 and 155.33 mg/kg) con-tributed to intense metabolic processes. Conclusions: S. aplina could serve as a valuable source of natural antioxidants and enzyme inhibitors associated with glucose and lipid metabolism, suggesting its promising application in the prevention and management of metabolic disorders.. Full article

Background: Thromboelastography-6s (TEG^®6s), a novel device developed to assess coagulation status, presents advantages such as less frequent calibration, ease of use, and greater stability against movements compared to the previous system (TEG5000). This is the first study in the literature to compare coagulation profiles in the early postoperative period of liver resection (LR) using conventional coagulation tests (CCTs) and TEG^®6s. Methods: Forty-six adult patients admitted to the ICU post-surgery after elective LR for malignancy were included. CCTs were used to classify patients into hypocoagulable (HCG) (platelet count < 80 × 10⁹/L, international normalized ratio ≥ 1.4, or activated partial thromboplastin time > 38 s) and normocoagulable (all other cases) groups. Mann–Whitney tests, Spearman’s correlation, and linear regression were used. Results: On ICU admission, nineteen (41.3%) patients had a hypocoagulable profile based on CCTs, but only two (10.5%) of them were rated as hypocoagulable by TEG (p = 0.165). Intraoperatively, HCG patients experienced higher estimated blood loss (EBL) (p = 0.002); they required more fluids (p = 0.019), and more of them received red blood cell transfusions (p = 0.025). They also had higher postoperative arterial lactate levels (p = 0.036). Postoperative 12 h EBL was similar in the two groups (around 150 mL). The ICU stay was longer for HCG group (p = 0.010). Weak associations were observed between TEG/CCTs measures of coagulation initiation [e.g., between R time citrated rapid TEG, and international normalized ratio (r² = 0.448; p < 0.001)], clot formation [i.e., between conventional fibrinogen value using Clauss method and α-angle citrated rapid TEG (r² = 0.542; p < 0.001)], and clot strength [e.g., between conventional fibrinogen and citrated kaolin maximum amplitude (r² = 0.484; p < 0.001)]. Conclusions: CCTs revealed hypocoagulability that was not confirmed by TEG^®6s. However, the thromboelastography coagulation profile was more consistent with the detected non-relevant postoperative bleeding. Full article

With increasing natural gas processing demands, triethylene glycol (TEG) in dehydration systems becomes contaminated by gas-carried impurities, leading to problematic foaming, degradation, and significant glycol losses that compromise operational economics, pipeline integrity, and product quality. To systematically investigate impurity effects, we conducted comprehensive single-factor TEG regeneration experiments simulating field conditions. Through precise measurements of foaming height, defoaming time, and interfacial tension, we established clear correlations between impurity types and TEG foaming characteristics. Our results demonstrate a distinct hierarchy of foaming influence: chemical additives > solid impurities > water-soluble inorganic salts > MDEA > hydrogen sulfide > hydrocarbons. Chemical additives showed the most pronounced effect on surface tension, reducing it to 31.1 mN/m at 1500 mg/L. Water-soluble inorganic salts affected foaming through combined decomposition and crystalline morphology effects, ranked as MgCl₂ > NaHCO₃ > KCl > NaCl > Na₂SO₄ > CaCl₂ (MgCl₂ achieving 33.8 mN/m at 2000 mg/L). Solid impurity impacts correlated strongly with particle morphology (CaCO₃ > Fe₂O₃ > CaSO₄ > ZnO > CuO > Al₂O₃ > FeS), stabilizing at 1.5 mg/L. Hydrocarbons showed negligible influence, while hydrogen sulfide and MDEA caused only minor surface tension reductions with limited foaming effects. Based on these findings, we propose targeted mitigation strategies for industrial implementation. Full article

Efficient data transmission in low Earth orbit (LEO) satellite networks is critical for supporting real-time global communication, Earth observation, and numerous data-intensive space missions. A fundamental challenge in these networks involves solving the maximum flow problem, which determines the optimal data throughput across highly dynamic topologies with limited onboard energy and data processing capability. Traditional algorithms often fall short in these environments due to their high computational costs and inability to adapt to frequent topological changes or fluctuating link capacities. This paper introduces an accelerated maximum flow algorithm specifically designed for dynamic LEO networks, leveraging a prediction-enhanced approach to improve both speed and adaptability. The proposed algorithm integrates a novel energy-time expanded graph (e-TEG) framework, which jointly models satellite-specific constraints including time-varying inter-satellite visibility, limited onboard processing capacities, and dynamic link capacities. In addition, a learning-augmented warm-start strategy is introduced to enhance the Ford–Fulkerson algorithm. It generates near-optimal initial flows based on historical network states, which reduces the number of augmentation steps required and accelerates computation under dynamic conditions. Theoretical analyses confirm the correctness and time efficiency of the proposed approach. Evaluation results validate that the prediction-enhanced approach achieves up to a 32.2% reduction in computation time compared to conventional methods, particularly under varying storage capacity and network topologies. These results demonstrate the algorithm’s potential to support high-throughput, efficient data transmission in future satellite communication systems. Full article

Energy harvesting in the automotive sector is a rapidly growing field aimed at improving vehicle efficiency and sustainability by recovering wasted energy. Various technologies have been developed to convert mechanical, thermal, and environmental energy into electrical power, reducing dependency on traditional energy sources. This manuscript provides a comprehensive review of energy harvesting applications/methodologies, aiming to trace the research lines and future developments. This work identifies the main categories of harvesting solutions, namely mechanical, thermal, and hybrid/environmental solar–wind systems; each section includes a detailed review of the technical and scientific state of the art and a comparative analysis with detailed tables, allowing the state of the art to be mapped for identification of the strengths of each solution, as well as the challenges and future developments needed to enhance the technological level. These improvements focus on energy conversion efficiency, material innovation, vehicle integration, energy savings, and environmental sustainability. The mechanical harvesting section focuses on energy recovery from vehicle vibrations, with emphasis on regenerative suspensions and piezoelectric-based solutions. Specifically, solutions applied to suspensions with electric generators can achieve power outputs of around 1 kW, while piezoelectric-based suspension systems can generate up to tens of watts. The thermal harvesting section, instead, explores methods for converting waste heat from an internal combustion engine (ICE) into electrical power, including thermoelectric generators (TEGs) and organic Rankine cycle systems (ORC). Notably, ICEs with TEGs can recover above 1 kW of power, while ICE-based ORC systems can generate tens of watts. On the other hand, TEGs integrated into braking systems can harvest a few watts of power. Then, hybrid solutions are discussed, focusing on integrated mechanical and thermal energy recovery systems, as well as solar and wind energy harvesting. Hybrid solutions can achieve power outputs above 1 kW, with the main contribution from TEGs (≈1 kW), compared to piezoelectric systems (hundreds of W). Lastly, a section on commercial solutions highlights how current scientific research meets the automotive sector’s needs, providing significant insights for future development. For these reasons, the research results aim to be guidelines for a better understanding of where future studies should focus to improve the technological level and efficiency of energy harvesting solutions in the automotive sector. Full article