Search Results (19)

Weeds are unwanted and invasive plants characterized by their rapid growth and ability to compete with crops for essential resources such as space, water, nutrients, and sunlight. This competition has a negative impact on crop quality and productivity. To reduce the influence of weeds, precision weeding is used, which uses image sensors and computational algorithms to identify plants and classify weeds using digital images. This study used images of maize (Zea mays L.) to detect four types of weeds (Lolium rigidum, Sonchus oleraceus, Solanum nigrum, and Poa annua). For this purpose, YOLO (You Only Look Once) architectures, YOLOv8s, YOLOv9s, YOLOv10s, and YOLOv11s versions, were trained and compared, along with an architecture based on RT-DETR (Real-Time Detection Transformer), version RT-DETR-1. The YOLO architectures are noted for their real-time detection efficiency, and RT-DETR-l allows evaluation of the impact of an architecture that dispenses with Non-Maximum Suppression (NMS). The YOLOv9s model had the best overall performance, achieving a mAP@0.5 of 0.834 in 60 epochs and an F1-score of 0.78, which demonstrates a optimal balance between accuracy and recall, although with less confidence in its predictions. On the other hand, the RT-DETR-l model stood out for its efficiency in convergence, reaching a competitive performance in only 58 epochs with a mAP@0.5 of 0.828 and an F1-score of 0.80. Full article

The introduction of alternative crops, including wild edible and medicinal plants, in organic cultivation systems presents an attractive approach to producing healthy and high-quality products due to their content in beneficial compounds and increased nutritional value. The current study evaluated the impact of organic and conventional fertilization on the growth, quality, nutrient status and stress response of the two wild edible species, e.g., purslane (Portulaca oleracea L.) and common sowthistle (Sonchus oleraceus L.), under field conditions. The fertilization treatments included the following: a control (NoFert) treatment with no fertilizers added, base dressing with conventional fertilization (CoFert), base dressing with organic fertilization (OrFert), base dressing and side dressing with conventional fertilization (OrFert + S_CoFert) and base dressing and side dressing with organic fertilization (CoFert + S_CoFert). Organic fertilization was carried out using a commercial vinasse-based organic fertilizer. In both purslane and common sowthistle, the application of organic fertilizers provided comparable or even enhanced plant growth traits, macronutrient content (i.e., P and K for purslane, and N for sowthistle) and quality (i.e., total soluble solids) compared to the application of conventional fertilizers. On the other hand, conventional fertilization with supplementary fertilization positively influenced the plant growth of purslane (i.e., plant height and stems biomass), as well as its physiological parameters (i.e., chlorophylls content), total phenolics content and antioxidant capacity (i.e., DPPH and FRAP). Similarly, conventional fertilization led to increased total phenolics and antioxidants in common sowthistle, while variable effects were observed regarding plant physiology, stress response and antioxidant capacity indices. In conclusion, the use of organic fertilization in both purslane and common sowthistle exhibited a performance similar to that of conventional fertilization, although further optimization of fertilization regimes is needed to improve the quality of the edible products. Full article

Wild edible greens are a key ingredient of the so-called Mediterranean diet and they are commonly used in various local dishes in their raw or processed form. Domestic processing of edible greens may affect their nutritional value and chemical profile. In this work, six wild species (e.g., Cichorium spinosum L. (S1); Centaurea raphanina subsp. mixta (DC.) Runemark (S2); Picris echioides (L.) Holub (S3); Urospermum picroides (L.) Scop. ex. F.W. Schmidt (S4); Sonchus oleraceus L. (S5); and S. asper L. (S6)) were assessed for the effect of domestic processing (boiling) on chemical composition and bioactivities. Concerning the chemical composition, glucose, oxalic acid, α-tocopherol, and α-linolenic acid were the most abundant compounds, especially in P. echiodes leaves. After decoction, mainly sugars, tocopherols, and oxalic acid were decreased. The species and processing affected the phenolic compounds content and antioxidant, cytotoxicity, and anti-inflammatory activities. Specific compounds were not previously detected in the studied species, while hydroethanolic extracts contained a higher total phenolic compound content. Hydroethanolic and aqueous extracts were effective towards a range of bacterial and fungi strains. Therefore, the consumption of leaves has health-promoting properties owing to the bioactive compounds and can be integrated into healthy diets. However, domestic cooking may affect the chemical profile and bioactivities of the edible leaves, especially in the case of free sugars and phenolic compound content where a significant reduction was recorded in leaves after decoction. On the other hand, domestic processing could be beneficial since it reduces the oxalic acid content in edible leaves, which is considered an antinutritional factor. Full article

Sonchus oleraceus L. is a leafy vegetable that is usually consumed in the area of the Mediterranean and is a frequently used traditional herb to treat a variety of ailments. Previous studies deduced the potent antioxidant and cytotoxic functions of the different extracts and isolated compounds from S. oleraceus. The current study represents the first instance of chemical profiling and bioactivities of the extracted essential oil (EO) of S. oleraceus. The present investigation set out to identify the chemical components of this EO by means of Gas Chromatography with Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (G004-MS) techniques; assess the oil’s antioxidant potencies through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS) assays; and evaluate the oil’s cytotoxic impact against HepG2 cancer cell lines. The GC-MS chemical profiling revealed the identification of 23 components representing 97.43% of the total oil mass within abundant cyclic ketones (20.15%), nonterpenoidial hydrocarbons (28.77%), and sesquiterpenes (42.19%). The main components were n-nonadecane (28.77%), trans-caryophyllene (23.73%), trans-methyl dihydrojasmonate (19.55%), and cis-cadina-1,4-diene (9.44%). In a dose-dependent manner, this EO demonstrated antioxidant capacities on DPPH and ABTS, with IC₅₀ values of 609.35 and 804.16 µg/mL, respectively, compared to ascorbic acid. Using doxorubicin as a reference therapy, the MTT assay findings revealed that this oil had remarkable inhibitory effects on the proliferation of HepG2 cancer cell lines, with an IC₅₀ of 136.02 µg/mL. More studies were recommended for further investigation of new biological roles for this oil and its main components, along with the construction of action mechanisms based on chemical components. Full article

Introducing non- or under-utilized crops to cultivation generates benefits such as biodiversity enrichment, supporting mitigation actions towards climate change-induced effects. The salinization of soil and water supplies is progressively disrupting natural habitats and food production, especially in regions such as the Mediterranean. Sonchus oleraceus L. is a Mediterranean wild leafy green with nutritional and medicinal properties. This study’s purpose was to determine whether salinity affects the growth, quality, and nutrient composition of Sonchus oleraceus L. In an unheated plastic greenhouse, seedlings were transplanted in pots filled with perlite and irrigated with a nutrient solution with no NaCl added (the control, C) or with the addition of 40, 60, 80, and 100 mM of NaCl (treatments S4, S6, S8, and S10, respectively). The leaf and root growth, leaf quality, and the nutrient composition of leaves and roots were determined. Regarding the results, growth was mainly affected at high salinity levels (S8 and S10), with no observed effects of salinity on the determined quality parameters. The nutrient composition was variably affected by salinity in leaves but not in roots (except in the case of Na and the K/Na ratio). Sonchus oleraceus L. showed a general relative tolerance in moderate salinity levels (40 and 60 mM of NaCl), suggesting potential commercial exploitation of the species in areas where the quality of irrigation water is low. However, the health effects of consuming this species grown under salinity stress need to be studied in future research. Full article

Corn (Zea mays L.) is one of the most important cereals worldwide. To maintain crop productivity, it is important to eliminate weeds that compete for nutrients and other resources. The eradication of these causes environmental problems through the use of agrochemicals. The implementation of technology to mitigate this impact is also a challenge. In this work, an artificial vision system was implemented based on the YOLOv5s (You Only Look Once) model, which uses a single convolutional neural network (CNN) that allows differentiating corn from four types of weeds, for which a mobile support structure was built to capture images. The performance of the trained model had a value of mAP@05 (mean Average Precision) at a threshold of 0.5 of 83.6%. A prediction accuracy of 97% and a mAP@05 of 97.5% were obtained for the maize class. For the weed classes, Lolium perenne, Sonchus oleraceus, Solanum nigrum, and Poa annua obtained an accuracy of 86%, 90%, 78%, and 74%, and a mAP@05 of 81.5%, 90.2%, 76.6% and 72.0%, respectively. The results are encouraging for the construction of a precision weeding system. Full article

Notwithstanding the increased interest in wild edible plants, little is known on how some domestic thermal processes can affect their content. The aim of this study was to investigate the amounts of minerals, B1 and B2 vitamins, tocols, and carotenoids in raw, boiled, and steamed wild edible plants, namely, Sonchus asper (L.) Hill s.l., Sonchus oleraceus L., Cichorium intybus L., and Beta vulgaris L. var cicla. All vegetables were confirmed as high sources of lutein (from 6 to 9 mg/100 g) and β-carotene (from 2 to 5 mg/100 g). Quite high amounts of violaxanthin and neoxanthin were found. Alfa-tocopherol and γ-tocopherol were the main tocols, with same contents in raw and processed vegetables (about 2.5 mg/100 g). The most abundant macro element and trace element were, respectively, potassium and iron. B1 and B2 vitamins were found in low amounts in almost all plants, with the exception of thiamine in Beta vulgaris (about 1.6 mg/100 g). Boiling led to a significant loss of minerals (up to 60%) and B-group vitamins (up to 100%), while, among carotenoids, it only affected violaxanthin levels (up to 90%). Steamed vegetables showed only a slight reduction, about 20%, in β-carotene and lutein, with a marked decrease in violaxanthin and neoxanthin. One hundred grams of all fresh and cooked plants can be claimed as a source of vitamin A and E. Full article

Determining the amount of heat units required to kill weed seeds is a crucial aspect for the success of weed control through soil solarization. Lab experiments were designed to determine the duration of exposure for weed seeds that is required to suppress germination at temperatures (40, 45, 50, 55, and 60 °C) in the range of those typically achieved during soil solarization in California. The species tested were annual sowthistle (Sonchus oleraceus L.), bristly oxtongue (Picris echioides L.), nettleleaf goosefoot (Chenopodium murale L.), redroot pigweed (Amaranthus retroflexus L.), common purslane (Portulaca oleracea L.), little mallow (Malva parviflora L.), and redstem filaree (Erodium cicutarium L.). Germination tests were performed to assess the germinability of the weed seeds. The germination suppression by the lab-simulated solarization temperatures differed among the species based on their seasonality. The cool-season annuals S. oleraceus and P. echioides were more susceptible to the heat treatments than the warm-season annuals P. oleracea, A. retroflexus, and C. murale. The hard-seeded weed species M. parviflora and E. cicutarium were the least susceptible to the heat treatments. The germination rates of S. oleraceus, P. echioides, and C. murale were reduced at all of the temperatures that were tested. The germination rates for A. retroflexus and M. parviflora were not affected by temperatures below 40 °C. The germination rates for P. oleracea were not affected by temperature below 45 °C and the germination of E. cicutarium was not affected by any of the temperatures that were tested. The duration (hours) of exposure and percent of germination suppression of the weed seeds were used to create thermal-time hazard models for weed seeds using logistic regression. Full article

The present work investigates the nutritional and bioactive composition, as well as the organoleptic and sensory properties, of S. oleraceus and S. tenerrimus, two wild plant species traditionally used in the gastronomy of the Mediterranean area. Additionally, the effect of cultivation on leaf composition was assessed to explore their potential for large-scale production and commercialization from the point of view of possible losses or gains in quality. Both species were characterized as a good source of bioactive compounds, such as vitamins, pro-vitamins and carotenoids, with health-promoting and antioxidant properties that are highly appreciated. The sensory profile revealed a good general acceptance of S. oleraceus and S. tenerrimus, indicating that they could be included in the diet. Although the cultivation of S. oleraceus resulted in a decrease in the concentration of phenolic compounds when compared to wild-harvested plants, the opposite occurred for vitamin C. In S. tenerrimus, cultivation also increased the concentration of other compounds with important nutritional and healthy properties, such as sugars, organic acids and β-carotene. The results of the composition, organoleptic and sensory properties of S. oleraceus and S. tenerrimus support the idea of their potential to be used as edible leafy vegetables and as promising assets for functional foods. Full article

A substantial quantity of solid waste that is high in phenolics and biocomponents is produced during the industrial manufacturing of essential oils (EOs); this presents an important management challenge for the EO sector. This currently produces a significant amount of residue, causing issues of disposal and management and the impact that the residues have on both the environment and human health. The present study evaluated the potential use of Origanum dubium Boiss. residues (ODR) and Sideritis cypria Post. residues (SCR) derived via distillation at different levels (0–5–10–20–40% v/v) for use in partial peat substitution in the production of Sonchus oleraceus L. (sowthistle) plants. Both ODR and SCR accelerated the pH, electrical conductivity, organic matter content, and mineral content of the growing media, but also negatively affected several of the physical characteristics of the media, such as the total porosity and aeration. This resulted in decreased plant growth, which was more noticeable at the high residue ratios. Plants responded to this by decreasing the leaf stomatal conductance, decreasing the chlorophyll content at 40% ODR and 20% SCR mixtures, and activating several non-enzymatic (phenols, flavonoids, and antioxidant capacity) and enzymatic (superoxide dismutase) mechanisms to challenge the observed stress conditions, as indicated by lipid peroxidation and the hydrogen peroxide increase. Plants grown in residue media exhibited changes in mineral accumulation, even though both ODR and SCR were rich in minerals. It may be concluded that ODR and SCR, when employed at low levels of 10% and 20%, respectively, have the potential for use in the preparation of growing media as they may increase plant material antioxidants, but further improvement of the growing media’s properties is needed to ensure adequate yield. Full article

Due to the various hazards of using synthetic chemical compounds in pharmaceutics, agriculture, and industry, scientists and researchers do their best to explore and assess new green natural compounds from natural resources with potent activity. The essential oil (EO) from the resin collected from Araucaria heterophylla Salisb. was extracted by the microwave technique and chemically characterized via GC-MS analysis. Furthermore, the extract EO was assessed for its antioxidant and phytotoxic activities. The EO has 33 compounds, mainly terpenes (98.23%), and the major compounds were α-pinene (62.57%), β-pinene (6.60%), germacrene D (5.88%), and β-caryophyllene (3.56%). The extracted EO showed substantial antioxidant activity, where it showed IC₅₀ values of 142.42 and 118.03 mg L⁻¹ for DPPH and ABTS, respectively. On the other hand, the EO revealed considerable phytotoxicity against the weed Chenopodium murale, where the EO showed IC₅₀ values of 304.0, 230.1, and 147.1 mg L⁻¹, for seed germination, seedling shoot growth, and seedling root growth, respectively. Moreover, the EO showed the same pattern of allelopathic inhibition against the weed Sonchus oleraceus, where it showed IC₅₀ values of 295.7, 224.5, and 106.1 mg L⁻¹, for seed germination, seedling shoot growth, and seedling root growth, respectively. The present study showed that the extraction technique affects the constituents of the EO, particularly the quantitative composition. The EO of A. heterophylla resin also revealed considerable antioxidant and phytotoxic activity against weeds. Therefore, it can be considered a promising natural resource that could be integrated into the weed management approach. However, further study is recommended for deep characterization of their authentic compounds and evaluation of their mode of action(s) on a wide spectrum of weeds. Full article

Paraphoma radicina is a new pathogen that causes alfalfa paraphoma root rot (APRR), leading to alfalfa production losses. The resistance levels of 30 alfalfa cultivars to APRR have already been characterized. However, the pathogenic mechanism of P. radicina is still unclear. This study aimed to assess the effects of a crude toxin extracted from P. radicina cell-free culture filtrate (CFCF) on susceptible and resistant cultivars of alfalfa. Meanwhile, the crude toxin components were detected using gas chromatography-mass spectrometry (GC-MS) analysis. CFCF cultured in MEB medium for 14 days and crude toxin extracted by ethyl acetate induced significant phytotoxicity caused the average lesion areas of 5.8 and 3.9 mm², respectively, on alfalfa leaves. The crude toxin exhibited resistance to high temperature, as shown by a lesion area of 3.6 mm² when treated at 120 °C for 30 min. Different concentrations of the crude toxin in water and MS medium had different effects on susceptible and resistant cultivars. Moreover, the crude toxin affected the plasma membrane, mitochondria, and nuclear membranes of alfalfa root cortical cells. Further, it induced significant phytotoxicity on Sonchus oleraceus L., Capsella bursa-pastoris (Linn.) Medic, and Chenopodium album L. Agropyron cristatum L. (average lesion areas; 11.6, 15.8, 21.4, and 6.2 mm², respectively), indicating that the crude toxin of P. radicina is a non-host-selective toxin. GC-MS analysis detected four possible active substances in the toxin (3-hydroxypyridine, 5-methylresorcinol, 3-Hydroxypropionic acid, and 4-Hydroxyphenylethanol). Therefore, this study may provide insight into the pathogenic mechanism of P. radicina to alfalfa. Full article

Wild edible plants of the Mediterranean represent an extraordinary food source and are basic ingredients in the “Mediterranean diet”. However, there is a scarcity of information about their commercial interest or cultivation practices. This study was conducted to evaluate how different amounts of inorganic and organic fertilization affected the plant growth parameters of Sonchus oleraceus and Portulaca oleracea. The experiment was performed in greenhouse conditions in pots containing soil, sand and vermiculite in a volume ratio of 1-1-1 for 12 weeks. The control treatment had no fertilization; the inorganic fertilization (N-P-K) was 100 mL of 100-100-100 mg L⁻¹; 300-100-100 mg L⁻¹; 600-100-100 mg L⁻¹; 300-200-100 mg L⁻¹; 300-300-100 mg L⁻¹; 300-200-200 mg L⁻¹; 300-200-300 mg L⁻¹; and 100 mL of the organic compost extract to reach same equivalence as 300 mg L⁻¹ of N and 100 mL of the organic compost extract + P inorg (equivalent to 300 mg L⁻¹ of N and 200 mg L⁻¹ of P). All treatments were applied weekly. All treatments assayed showed significantly higher leaf and stem weights compared to the control treatment; the highest values were recorded for the treatment of 600-100-100 in both plant species (S. oleraceus and P. oleracea). Treatment (600-100-100) showed a significantly higher N content, but not P and K contents, than the rest of the treatments. The plant nutrient contents of Mg, Fe, S and Ca did not differ among the tested fertilization regimes. The soil nutrient contents (N, P, K, Fe, Ca) showed no differences between treatments. We conclude that nitrogen had a main role in improving the plant growth parameters and yield in both wild plant species, the beneficial effect depending on the doses and origin (inorganic or organic) of the fertilizer applied. Full article

The glasshouse study was conducted with the objectives of (i) investigating the effect of soil moisture variations on the control efficiency of glyphosate on windmill grass (Chloris truncata R.Br.), common sowthistle (Sonchus oleraceus L.), and flaxleaf fleabane [Conyza bonariensis (L.) Cronq.], (ii) evaluating the tolerance of tested weed species under soil moisture variations, and (iii) determining the morphological and physiological characteristics of these species to partially explain herbicide tolerance under periods of reduced soil moisture availability (RSM). The species’ tolerance to glyphosate increased significantly under reduced soil moisture availability (p < 0.001). The lethal dose to cause herbicide injury or biomass reduction by 50% (LD₅₀) and 80% (LD₈₀) in relation to untreated control for water-stressed plants [i.e., moderate soil moisture availability (MSM) and RSM] was significantly higher than that of plants grown under high soil moisture availability (HSM). The tolerance factor (TF) for C. truncata, S. oleraceus, and C. bonariensis, in terms of biomass reduction under RSM, was 2.6, 2.4, and 2.6, respectively, as compared to HSM. The results showed that the glyphosate sensitivity, especially at the sub-lethal rates, of the three weed species under study decreased as soil moisture availability reduced (p< 0.01). Overall glyphosate efficacy, in relation to the recommended rate, was unaffected, except for C. truncata; the weed survived the highest tested glyphosate rate [750 g active ingredient (a.i.) ha⁻¹] under RSM. There was significant interaction between weed species and soil moisture regimes for weed morpho-physiological traits (p < 0.001), with reduced soil moisture having a more influential impact on the growth of C. bonariensis and S. oleraceus compared to C. truncata. Changes in the leaf characteristics, such as increased leaf thickness, higher leaf chlorophyll content, reduced leaf area, and limited stomatal activity for all the tested weed species under MSM and RSM in relation to HSM, partially explain the tolerance of species to glyphosate at sublethal rates. Full article

Concerns about a global decline in pollinators have called for more knowledge about the factors influencing wild pollinator abundance and diversity in agroecosystems. Agricultural intensification has been identified as the main cause of this “global pollinator crisis”, particularly due to reductions in natural areas holding critical floral and nesting resources. Maintaining native wild plants in agricultural landscapes (e.g., in field margins) is often recommended as a cost-effective and efficient method for pollinator conservation. In this study, the role of common wild flowers, often considered weeds, in supporting pollinators in a Mediterranean agroecosystem was investigated. This work involved a two-year field trial to compare five native weed species common in Mediterranean cereal agroecosystems: Convolvulus arvensis L., Daucus carota L., Malva sylvestris L., Papaver rhoeas L., and Sonchus oleraceus L. The goal was to compare the attractiveness of these species, and a mixture of all five, to different flower-visiting insect groups in order to assess their value in supporting wild pollinators. Overall, D. carota had the highest number of insect visits, followed by P. rhoeas. C. arvensis, M. sylvestris, and S. oleraceus, which had lower numbers of visits. On the basis of their overall attractiveness to pollinators and low risk for invasiveness, D. carota, P. rhoeas, and M. sylvestris are the most likely to contribute positively to the conservation of pollinators in agroecosystems. Our results also suggest that it is advantageous for wild flowers sown for the purpose of pollinator conservation to be grown in clumps, rather than highly intermingled, for improved visitation rates. Full article