Chemical Composition, Antioxidant, and Cytotoxic Activity of Essential Oils in the Above-Ground Parts of Sonchus oleraceus L.

Sonchus oleraceus L. is a leafy vegetable that is usually consumed in the area of the Mediterranean and is a frequently used traditional herb to treat a variety of ailments. Previous studies deduced the potent antioxidant and cytotoxic functions of the different extracts and isolated compounds from S. oleraceus. The current study represents the first instance of chemical profiling and bioactivities of the extracted essential oil (EO) of S. oleraceus. The present investigation set out to identify the chemical components of this EO by means of Gas Chromatography with Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (G004-MS) techniques; assess the oil’s antioxidant potencies through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS) assays; and evaluate the oil’s cytotoxic impact against HepG2 cancer cell lines. The GC-MS chemical profiling revealed the identification of 23 components representing 97.43% of the total oil mass within abundant cyclic ketones (20.15%), nonterpenoidial hydrocarbons (28.77%), and sesquiterpenes (42.19%). The main components were n-nonadecane (28.77%), trans-caryophyllene (23.73%), trans-methyl dihydrojasmonate (19.55%), and cis-cadina-1,4-diene (9.44%). In a dose-dependent manner, this EO demonstrated antioxidant capacities on DPPH and ABTS, with IC50 values of 609.35 and 804.16 µg/mL, respectively, compared to ascorbic acid. Using doxorubicin as a reference therapy, the MTT assay findings revealed that this oil had remarkable inhibitory effects on the proliferation of HepG2 cancer cell lines, with an IC50 of 136.02 µg/mL. More studies were recommended for further investigation of new biological roles for this oil and its main components, along with the construction of action mechanisms based on chemical components.


Introduction
Using plants therapeutically to treat a variety of illnesses is an old practice that has recently regained popularity.Medicinal plants provide more than half of the drugs used in the therapeutic treatment of cancer [1].
Essential oils (EOs) possess a volatile, fragrant, and highly complex mixture of mainly terpenoid components.Monoterpenes, sesquiterpenes, and aromatic compounds constitute the vast majority of the mixture.Steam distillation is the primary method used to deliver the EOs [2].EOs show a range of activities, including anticancer effects [3].Numerous EOs have been found to have antioxidant qualities, and their application as a natural antioxidant is growing in popularity due to current worries about possible health risks connected to Plants 2024, 13, 1712 2 of 14 some synthetic antioxidants.For food goods, adding EOs directly through mixing, active storage, or food-safe coatings could be a good substitute to stop autoxidation and increase shelf life [4,5].
The Asteraceae family is one of the largest flowering plant groups in the world, comprising roughly 1600 genera and 2500 species worldwide.Its most well-known taxa are lettuce, artichokes, chicory, daisies, and dandelion.People have used Asteraceae plants for food and medicine for millennia [6,7].Due to their unique phytochemical components, which include terpenes, polyphenolic compounds, lignans, saponins, sterols, and polysaccharides in addition to EOs, some of these taxa exhibit a variety of pharmacological characteristics [7].
Sowthistle, or S. oleraceus L., is a leafy vegetable that is commonly eaten in Mediterranean countries [8].Its leaves and soft, fresh stems are the only edible portions of Sonchus oleraceus (Asteraceae) that can be added to stews or salads [8].The aerial parts of this plant have been utilised by herbalists in Cameroon for the treatment of arthritis, infections, inflammation, headaches, diabetes, stomach pain, liver infection, and general nuisances [9,10].S. oleraceus has a wide range of biological characteristics that have been investigated previously and documented, especially anti-inflammatory, anti-diabetic, and antioxidant activities [9,11,12].There are no published studies on the chemical composition and/or biological characteristics of EO derived from S. oleraceus.
Therefore, the present study deals with (i) the identification of the chemical composition of the EO, derived by the hydro-distillation of the above-ground parts of S. oleraceus via Gas Chromatography with Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) techniques; (ii) the assessment of antioxidant activity against the DPPH and ABTS free radicals; and (iii) the evaluation of the cytotoxic activity of this oil against liver (HepG2) cancer cell lines.

Plant Materials
Above-ground parts of S. oleraceus (Figure 1) were collected in May 2021 throughout the plant's flowering period from the Nile Delta region of Menoufia (30.3502780332799, 30.869978698312085),Egypt.Dr. Ahmed Abd ElGawad, a taxonomy professor from Mansoura University, verified the identity of the plant.A voucher specimen (SO-xq-774z/21-02712) was stored in the Herbarium of the Faculty of Science, Mansoura University.

Isolation of the EO
Sonchus oleraceus's (200 g) fresh above-ground portions were chopped and then hydro-distilled in a 2 L round flask employing Clevenger apparatus.Following three hours of hydro-distillation, the plant's extracted EO was poured out, sorted, and quickly dried with 0.5 g of anhydrous sodium sulphate.Three separate plant samples, each weighing 200 g, underwent the same procedures.The three extracted EO samples were stored in three different vials of glass in the refrigerator until the starting point of the chemical and biological analyses.To find the EO yields (v/w%), the subsequent formula was utilised:

Gas Chromatography with Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis
The isolated EO samples were analysed according to the same protocols described before by Abd-ElGawad et al., 2023 [13] and El Gendy et al., 2024 [14].In brief, The GC-FID analysis was performed using an HP-5890 series II apparatus (Hewlett-Packard, Palo Alto, CA, USA) set with two silica capillary columns (30 m 0.25 mm; film thickness: 0.25 m), HP-Wax, and DB-5 (Agilent, Santa Clara, CA, USA).The temperature of the oven was

Isolation of the EO
Sonchus oleraceus's (200 g) fresh above-ground portions were chopped and then hydrodistilled in a 2 L round flask employing Clevenger apparatus.Following three hours of hydro-distillation, the plant's extracted EO was poured out, sorted, and quickly dried with 0.5 g of anhydrous sodium sulphate.Three separate plant samples, each weighing 200 g, underwent the same procedures.The three extracted EO samples were stored in three different vials of glass in the refrigerator until the starting point of the chemical and biological analyses.To find the EO yields (v/w%), the subsequent formula was utilised: 2.3.Gas Chromatography with Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis The isolated EO samples were analysed according to the same protocols described before by Abd-ElGawad et al., 2023 [13] and El Gendy et al., 2024 [14].In brief, The GC-FID analysis was performed using an HP-5890 series II apparatus (Hewlett-Packard, Palo Alto, CA, USA) set with two silica capillary columns (30 m 0.25 mm; film thickness: 0.25 m), HP-Wax, and DB-5 (Agilent, Santa Clara, CA, USA).The temperature of the oven was planned to rise from 60 • C to 220 • C at a rate of 5 • C per minute (with the carrier gas N 2 at 2 mL/min; spitless injecting), with the injector and detector set at 250 • C.
The apparatus used for the Gas Chromatography-Mass Spectrometry (GC-MS) analysis was an ISQTM Single Quadrupole Mass Spectrometer acting as the detector and a TRACE GC Ultra Gas Chromatograph from ThermoFisher Scientific, located in Waltham, MA, USA.A TR-5 MS column with a length of 30 m, an inner diameter of 0.32 mm, and a film thickness of 0.25 µm was fitted with the GC-MS system.Helium was employed as the carrier gas, flowing at a rate of 1.0 mL min −1 , with a split ratio of 1:10 until 240 • C was attained, following which the temperature increased at a rate of 4.0 • C per minute for a duration of one minute from 60 • C. At 210 • C, the injector and detector were maintained.The injections employed 1 µL of diluted EO samples in the mixes (1:10 hexane, v/v).Electron ionisation (EI) at 70 eV and the spectral range of m/z 40-450 were used to acquire mass spectra.
The identity and verification of the chemical components were performed using the NIST database, Wiley spectrum collection, AMDIS software (Automated Mass Spectrum Deconvolution and Identification; version 2.7) [15], and retention indices of n-alkanes (C 8 -C 22 ).In addition, the constituents were identified by comparing the components' retention times with those of genuine samples using the components' linear retention indices in relation to the n-hydrocarbon sequence and computer matching against commercial and homemade library mass spectra composed of known oil components, authentic mass spectra, and MS literature data.For GC-MS analysis, the following compounds were used as genuine examples: camphene, (α-and β-) pinene, D-limonene, linalool, α-terpineol, geraniol, citral, α-elemene, α-copaene, β-germacrene D, caryophyllene, cubedol, spathulenol, and α-eudesmol.

DPPH Assay
The ability of the isolated S. oleraceus EO to scavenge DPPH radicals was investigated according to the reported method by Miguel et al., 2010 [16].A variety of EO concentrations of 62.5, 125, 250, 500, and 1000 µg mL −1 were produced using ethanol as the solvent.After preparing equal parts of freshly synthesised DPPH (0.3 mM) and EO, they were vigorously mixed and left to stand at room temperature in the dark for 20 min.The absorbance at 517 nm was then found using a spectrophotometer (Analytik Jena, Jena, Germany).

ABTS Assay
The antioxidant capacity of S. oleraceus EO was evaluated following the established method based on the reduction in ABTS [17].The EO concentrations ranged from 65.5 to 1000 µg mL −1 and were produced in ethanol in accordance with the DPPH test protocol.In summary, the mixture was incubated for 6 min in the dark after 2 mL of freshly produced ABTS and 0.2 mL of each oil concentration were added.Utilising the Spectronic 21D spectrophotometer (Milton Roy, CA, USA), the colour absorbance at 734 nm was determined.Moreover, positive control with a dosage ranging from 12.5 to 225 µg mL −1 was made using vitamin C. For the DPPH and ABTS, the percentage that c referred to scavenging performance was established with the following formula: Plants 2024, 13, 1712 5 of 14 where A oil : absorbance of the oil samples, and A cont .: absorbance of control.

Cytotoxic Activity of S. oleraceus EO 2.5.1. Cell Culture
The cytotoxic activities of S. oleraceus EO were evaluated against the growth of the human cancer cells HepG2 (human hepatocellular carcinoma) (ATCC, Manassas, VA, USA).The cell type was cultivated at 37 • C in a 5% CO 2 humidified atmosphere using MEM medium supplemented with 10% foetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin [18].

MTT Assay
Using the MTT (3-[4,5,[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay [19], the cytotoxic activity of the S. oleraceus EO against the human cancer cell, HepG2, was examined.EO concentrations of 0.1, 1.0, 10.0, and 100 µg/mL were created when 5 × 10 4 cells/mL were seeded onto the 96-well microplate with 100 µL of culture media supplemented with 10% foetal bovine serum for the tests and then the mixture was incubated for 18-24 h at 37 • C with 5% CO 2 .Following the incubation period, the culture medium was disposed of, and 100 µL of the culture medium containing the measured concentrations of each EO concentration was incubated for 48 h at 37 • C with 5% CO 2 .Following incubation, the culture medium was disposed of, and 100 µL of the culture medium containing 0.5 mg/mL MTT was added to each well.The wells were then incubated for four hours at 37 • C with 5% CO 2 .Following the incubation period, the culture media was disposed of, and 100 µL was added to each well to lyse the cells.Using a microplate reader Model 680 (BioRad, Hercules, CA, USA), the absorbance of the converted dye was measured at 570 nm with background subtraction at 620 nm.The following formula was used to determine the percentage of cell viability and inhibition: where A treated: absorbance of the treated cells; and A control: the absorbance of control cell.
Inhibition % = 1 − A treated A control IC 50 : an oil concentration that inhibited cell growth by 50%.The studies were carried out in triplicate, and Prism Graphpad (version 5.0) software was utilised to compute the analytical parameter, which was 50% inhibition of cell growth (IC 50 ).

S. oleraceus EO Chemical Profiling
The hydro-distillation of S. oleraceus plant's above-ground parts afforded viscous oil with a pale-yellow colour and an overwhelming scent.The oil yield was 0.49% v/w.The plants belonging to the family Asteraceae were characterised by a high yield of EOs such as Achillea collina Becker ex Heimerl s.l.(0.73%, v/w) [20], Artemisia sieberi Besser (0.56-1.02, v/w) [21], Achillea fragrantissima (Forssk.)Sch.Bip.(0.84-0.96%, v/w) [22], and Pulicaria undulata L. (0.36-0.43%, v/w) [23].The current study's EO yielding was found to be higher than S. arvensis subsp.uliginosus's oil yielding (0.002%) [24].The quantitative and qualitative analysis of the extracted EO was carried out via GC-FID and GC-MS.Figure 2 presents the total ion chromatogram (TIC) of the analysed EO, along with the main components.Based upon these analyses, the chemical volatile constituents were identified and are listed in Table 1 along with their retention times (Rt.) and relative concentrations (Rel.Conc.), in addition to reported and calculated values of Kovats indexes (KIs).Twentythree compounds were identified, representing 97.43% of the overall oil mass.Ten classes of compounds were characterised, including benzenoids, cyclic ketones, fatty acid esters, hydrocarbons, oxygenated hydrocarbons, monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, and carotenoid-derived compounds.Based upon these analyses, the chemical volatile constituents were identified and are listed in Table 1 along with their retention times (Rt.) and relative concentrations (Rel.Conc.), in addition to reported and calculated values of Kovats indexes (KIs).Twentythree compounds were identified, representing 97.43% of the overall oil mass.Ten classes of compounds were characterised, including benzenoids, cyclic ketones, fatty acid esters, hydrocarbons, oxygenated hydrocarbons, monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, and carotenoidderived compounds.The predominant constituents of this oil were identified as sesquiterpenes, with a relative concentration of 42.19%.Sesquiterpene hydrocarbons accounted for 33.17% of the whole, while oxygenated sesquiterpenes (9.02%) were identified as being present in low relative concentrations.The total number of identified sesquiterpene hydrocarbons were trans-caryophyllene and cis-cadina-1,4-diene (cubenene), with corresponding relative concentrations of 23.73% and 9.44%.The documented findings of Kanaani and Mohamadi Sani (2015) [26], who revealed that these compounds are present in the EO obtained from Sonchus arvensis but as minor constituents, were fully supported by the current data.The two sesquiterpene hydrocarbons, trans-caryophyllene and cubenene, were commonly distributed in the EOs of several plants such as Guibourtia ehie (A.Chev.)J Léonard and Oricia suaveolens (Engl.)Verd.[27], Xanthium strumarium [28], and Gnaphalium elegans [29].The oxygenated sesquiterpenes were represented in this study by only two compounds, procerin and curcumenol, with relative concentrations of 6.95% and 2.07%, respectively.Procerin was a rarely reported compound in the EOs derived from plants, including Atractylodes macrocephala Koidz [30] and Salvia rosmarinus Spenn.[31].The EI-MS spectral chromatogram of the rare compound procerin is presented in Figure 3.
relative amounts of 1.83% and 0.74%, respectively.The two isomers of damascene, Z-βdamascone (1.78%) and Z-α-damascone (0.03%) were the carotenoid components that were found overall.Conversely, a total of five compounds were identified as monoterpene components, with α-thujaplicin (0.66%) being the predominant component and four additional compounds being detected as minors (0.01-0.03%).Seselin was detected as a main benzenoid in the EO derived from some plants, including Clausena excavate Burm.f.[40] and Carum roxburghianum [41], and a minor component in the EO of Clausena harmandiana (Pierre) Guillaumin [40].The EI-MS spectral chromatogram of this identified compound, seselin, is presented in Figure 3.The qualitative and quantitative differences between the EO of S. oleraceus's aboveground parts in the present work and the previous data of other Sonchus plants might be directly ascribed to the differences between the plant itself and the plant parts, in addition to the climatic conditions of the collection area for each plant [13].
Other non-terpenoid compounds were categorised as cyclic ketones (20.17%), cyclic benzenoids (2.61%), and fatty acid esters (1.12%).The trans-methyl dihydrojasmonate (19.55%), trans-jasmonol (0.60%), and γ-nonalactone (0.02%) were the only identified cyclic ketones.trans-methyl dihydrojasmonate was described as a significant bioactive compound in several plants, including Kitaibelia balansae [38] and Litsea petiolata Hook.f. [39].Five benzenoid compounds were identified within seselin as major compounds (1.72%) in addition to minor relative concentrations of benzyl benzoate, vanillin, benzyl acetate, and p-methylanisole.Also, the fatty acid esters were found in S. oleraceus's EO with low relative concentrations of only one compound, isopropyl myristate.Carotenoid-derived compounds and monoterpenes were discovered to be minor components, with relative amounts of 1.83% and 0.74%, respectively.The two isomers of damascene, Z-β-damascone (1.78%) and Z-α-damascone (0.03%) were the carotenoid components that were found overall.Conversely, a total of five compounds were identified as monoterpene components, with α-thujaplicin (0.66%) being the predominant component and four additional compounds being detected as minors (0.01-0.03%).Seselin was detected as a main benzenoid in the EO derived from some plants, including Clausena excavate Burm.f.[40] and Carum roxburghianum [41], and a minor component in the EO of Clausena harmandiana (Pierre) Guillaumin [40].The EI-MS spectral chromatogram of this identified compound, seselin, is presented in Figure 3.The qualitative and quantitative differences between the EO of S. oleraceus's aboveground parts in the present work and the previous data of other Sonchus plants might be directly ascribed to the differences between the plant itself and the plant parts, in addition to the climatic conditions of the collection area for each plant [13].
Plants 2024, 13, x FOR PEER REVIEW 9 of 14 According to the ABTS assay results, the EO behaved as a dose-related scavenger in a similar scenario.Using oil concentrations ranging between 62.50, 125.00, 250.00, 500.00,and 1000.00 µg/mL, respectively, the ABTS colours dropped to 26.18%, 34.95%, 44.72%, 50.57%, and 59.74%.The ABTS colours were similarly diminished in the range of 42.60 to 85.03% (Figure 4), with dosages that varied from 12.5 to 125.0 µg/mL for the reference standard.
Consequently, the EO showed DPPH-and ABTS-scavenging functions, with IC50 values of 609.35 and 804.16 µg mL −1 , respectively (Figure 4).Conversely, ascorbic acid displayed IC50 values of 43.39 and 23.55 µg mL −1 , respectively.These findings revealed that this oil has good free-radical-scavenging capacities compared with ascorbic acid, in addition to comparing this with the documented antioxidant results of EOs of other plants [42][43][44][45].The current results demonstrate the antioxidant abilities of S. oleraceus's EO that were directly attributed to the chemical constituents, especially the main components.Through synergistic and/or single routes, the primary components of plant extracts and EOs served as radical scavengers [46,47].The scavenging of DPPH and ABTS radicals depends upon the electron accepting [48,49] and transferring [50] routes, respectively.According to the ABTS assay results, the EO behaved as a dose-related scavenger in a similar scenario.Using oil concentrations ranging between 62.50, 125.00, 250.00, 500.00,and 1000.00 µg/mL, respectively, the ABTS colours dropped to 26.18%, 34.95%, 44.72%, 50.57%, and 59.74%.The ABTS colours were similarly diminished in the range of 42.60 to 85.03% (Figure 4), with dosages that varied from 12.5 to 125.0 µg/mL for the reference standard.
Consequently, the EO showed DPPH-and ABTS-scavenging functions, with IC 50 values of 609.35 and 804.16 µg mL −1 , respectively (Figure 4).Conversely, ascorbic acid displayed IC 50 values of 43.39 and 23.55 µg mL −1 , respectively.These findings revealed that this oil has good free-radical-scavenging capacities compared with ascorbic acid, in addition to comparing this with the documented antioxidant results of EOs of other plants [42][43][44][45].The current results demonstrate the antioxidant abilities of S. oleraceus's EO that were directly attributed to the chemical constituents, especially the main components.Through synergistic and/or single routes, the primary components of plant extracts and EOs served as radical scavengers [46,47].The scavenging of DPPH and ABTS radicals depends upon the electron accepting [48,49] and transferring [50] routes, respectively.
The free-radical-scavenging action of antioxidants is generally explained by three methods, including hydrogen proton transfer (HAT), sequential proton loss electron transfer (SPLET), and single electron transfer (SET-PT) [51].Herein, the oxygenated components within a remarkable concentration of this oil, ≈27%, acted as antioxidant agents via the free electrons on the oxygenated groups that increased the trapping of the free radicals.Additionally, the non-oxygenated constituents made a strong contribution to this activity by a singular and/or synergetic effect.trans-caryophyllene is a frequently encountered sesquiterpene present in several plant extracts and EOs, possessing associated antioxidant characteristics [52,53].Dahham and his co-workers found that trans-caryophyllene is a very active antioxidant agent with potent scavenging capacities of both BPPH and FRAP radicals [53].Furthermore, Eos extracted from Vernonia chalybaea Mart.ex DC. [54], Acquilaria crassna [53], Murraya paniculata [55], Melissa officinalis subsp.officinalis [56], and Tabernaemontana catharinensis A. DC.[57] were instances of potent antioxidant EOs that included trans-caryophyllene as a crucial component that scavenges radicals.Additionally, the main components, including trans-methyl dihydrojasmonate, n-nonadecane, and procerin, represented active radical-scavenging components of several EOs in some plants like Murraya paniculata [55], Melissa officinalis subsp.officinalis [56], Tabernaemontana catharinensis A. DC.[57], and Kitaibelia balansae [38].

Cytotoxic Effect
Utilising the conventional MTT process [58], the cytotoxic impact of EO on S. oleraceus's above-ground parts against the cell growth of HepG2 cell lines was assessed.The procedure consisted of experimenting with the EO at increasing concentrations (0.1, 1.0, 10.0, and 100.0 µg/mL) for 48 h and counting the entire number of living cells.The results (Table 2) revealed that the higher concentration of the EO at 100.0 µg/mL exhibited the highest cell viability of the cell lines within a value of 61.09%.Also, the current data demonstrated that this oil shows a dose-dependent activity against the cell growth of the HepG2 cell lines.The two doses at 1.0 and 0.1 µg/mL exhibited very low effects on the cell growth due to the cell viability of the cell lines within respective percentages of 90.45 and 94.03%.Table 2 displays the EO's concentration sufficient to inhibit 50% of the cell growth (IC 50 ).The findings demonstrated that this oil exhibits moderate cytotoxic potency within the value of IC 50 at 136.02 µg/mL compared with the reference drug, doxorubicin, within IC 50 at 0.23 ± 0.01.The moderate cytotoxic effect of this oil on the growth of HepG2 cancer cells might be ascribed to the chemical constituents, especially the major compounds such as transcaryophyllene, trans-methyl dihydrojasmonate, n-nonadecane, and procerin.The main trait of these compounds was their ability for electron donation and/or electron transfer, which can cause a nucleophilic attack on the DNA of the cancer cells and thus inhibit or limit cell growth [58].Based upon this fact, the inhibition of cell growth by these components was ascribed to their ability to donate electrons and thus cause the nucleophilic attack.The abilities of these compounds to have free electrons might be attributed to the following: (i) the presence of the double bonds in trans-caryophyllene and cis-cadina-1,4diene; (ii) the oxygenation in the trans-methyl dihydrojasmonate (-C=O and -COOCH 3 ); and (iii) the oxygenation and double bonds of procerin.The EOs of these plants, including some or all of these compounds as major components, were described as active against the growth of several cancer cell lines.The EOs of some plants, like Salvia officinalis [59], Zornia brasiliensis [60], and Zanthoxylum nitidum [61], were reported to be strong inhibitors of the cell growth of numerous cancer cells, including HepG2 cancer cells, due to the high concentrations of some or all of these compounds.Additionally, HepG2 cancer cell lines are susceptible to the cytotoxic effects of some of these compounds, such as trans-caryophyllene.trans-caryophyllene affects a number of key pathways in the pathophysiology of tumours, including those involving MAPK, PI3K, Akt, mTOR, S6 kinase 1 (S6K1), and the Signal Transducer and Activator of Transcription (STAT3) [62,63].

Conclusions
Sonchus oleraceus is an important edible plant within several traditional medicines in the Mediterranean area.The current findings represent a key to using the S. oleraceus EO and its major compounds as antioxidant and cytotoxic agents.The results of bioassays revealed that this oil exerts radical scavenger abilities of the radicals DPPH and ABTS.Also, this oil demonstrated moderate anti-proliferation via the inhibition of the cancer cells' growth of HepG2.These bioactivities might be attributed to the chemical components of this oil, especially the main compounds, including n-nonadecane, trans-caryophyllene, trans-methyl dihydrojasmonate, and cis-cadina-1,4-diene.The mechanism of the actions of this oil and/or its main compounds as radical scavengers and cytotoxic agents should be studied.

Figure 1 .
Figure 1.The herb Sonchus oleraceus L. (A) Plant shoot system, (B) close view of leaves, (C) close view of closed inflorescence (head), and (D) open inflorescence.

Figure 1 .
Figure 1.The herb Sonchus oleraceus L. (A) Plant shoot system, (B) close view of leaves, (C) close view of closed inflorescence (head), and (D) open inflorescence.

Figure 4 .
Figure 4.The antioxidant capacity of EO from S. oleraceus's above-ground sections was assessed using DPPH and ABTS tests at several dosages in comparison to the standard (ascorbic acid).*** Different letters inside each line curve indicate values significant at p 0.05 following Tukey's HSD test.

Figure 4 .
Figure 4.The antioxidant capacity of EO from S. oleraceus's above-ground sections was assessed using DPPH and ABTS tests at several dosages in comparison to the standard (ascorbic acid).*** Different letters inside each line curve indicate values significant at p 0.05 following Tukey's HSD test.

Table 1 .
Chemical components of essential oil derived from Sonchus oleraceus.

Table 1 .
Chemical components of essential oil derived from Sonchus oleraceus.
[25]Retention times, KI Lit : reported, and KI Exp .: calculated Kovats indexes.Authentication was accomplished by comparing the retention indices and components' mass spectral data with the NIST Mass Spectral Library, the Wiley Registry of Mass Spectral Data (8th edition, 2), and the literature[25].

Table 2 .
Cytotoxic effect (cell viability % and IC 50 ) of EOs of S. oleraceus's above-ground parts on HepG2 cancer cells.