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25 pages, 705 KB  
Article
Privacy-Preserving Set Intersection Protocol Based on SM2 Oblivious Transfer
by Zhibo Guan, Hai Huang, Haibo Yao, Qiong Jia, Kai Cheng, Mengmeng Ge, Bin Yu and Chao Ma
Computers 2026, 15(1), 44; https://doi.org/10.3390/computers15010044 - 10 Jan 2026
Viewed by 174
Abstract
Private Set Intersection (PSI) is a fundamental cryptographic primitive in privacy-preserving computation and has been widely applied in federated learning, secure data sharing, and privacy-aware data analytics. However, most existing PSI protocols rely on RSA or standard elliptic curve cryptography, which limits their [...] Read more.
Private Set Intersection (PSI) is a fundamental cryptographic primitive in privacy-preserving computation and has been widely applied in federated learning, secure data sharing, and privacy-aware data analytics. However, most existing PSI protocols rely on RSA or standard elliptic curve cryptography, which limits their applicability in scenarios requiring domestic cryptographic standards and often leads to high computational and communication overhead when processing large-scale datasets. In this paper, we propose a novel PSI protocol based on the Chinese commercial cryptographic standard SM2, referred to as SM2-OT-PSI. The proposed scheme constructs an oblivious transfer-based Oblivious Pseudorandom Function (OPRF) using SM2 public-key cryptography and the SM3 hash function, enabling efficient multi-point OPRF evaluation under the semi-honest adversary model. A formal security analysis demonstrates that the protocol satisfies privacy and correctness guarantees assuming the hardness of the Elliptic Curve Discrete Logarithm Problem. To further improve practical performance, we design a software–hardware co-design architecture that offloads SM2 scalar multiplication and SM3 hashing operations to a domestic reconfigurable cryptographic accelerator (RSP S20G). Experimental results show that, for datasets with up to millions of elements, the presented protocol significantly outperforms several representative PSI schemes in terms of execution time and communication efficiency, especially in medium and high-bandwidth network environments. The proposed SM2-OT-PSI protocol provides a practical and efficient solution for large-scale privacy-preserving set intersection under national cryptographic standards, making it suitable for deployment in real-world secure computing systems. Full article
(This article belongs to the Special Issue Mobile Fog and Edge Computing)
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19 pages, 6012 KB  
Article
Donor- and Acceptor-Side Protection Against Photosystem I Photoinhibition in Arabidopsis thaliana
by Marina Kozuleva
Int. J. Mol. Sci. 2026, 27(1), 9; https://doi.org/10.3390/ijms27010009 - 19 Dec 2025
Viewed by 312
Abstract
Photosystem I (PSI) photoinhibition (PI(I)) is gaining traction as a potentially more significant threat to plant performance than photoinhibition of photosystem II (PSII). The increased focus is facilitated by the implementation of specific protocols that induce PI(I), such as artificial fluctuating light (FL) [...] Read more.
Photosystem I (PSI) photoinhibition (PI(I)) is gaining traction as a potentially more significant threat to plant performance than photoinhibition of photosystem II (PSII). The increased focus is facilitated by the implementation of specific protocols that induce PI(I), such as artificial fluctuating light (FL) and repetitive short saturating pulses (rSPs). rSPs were long considered a specific sub-case of FL. However, recent evidence suggests that PI(I) proceeds via at least two distinct, treatment-dependent mechanisms, leading to damage at the donor or acceptor side of PSI. This discovery suggests that rSPs and FL represent distinct photoinhibitory stresses and that different mechanisms protect PSI against FL and rSPs. This study comparatively analyzed the effects of FL and rSPs on PSI activity in Arabidopsis thaliana wild-type plants and a selection of mutants (pgr5, pgrl1, stn7, tap38/pph1, and pgr1), previously noted or hypothesized to have altered PI(I) sensitivity relative to the wild type. The results of this work, particularly the contrasting sensitivity of tap38/pph1 compared to the wild type under FL and rSP conditions, strongly suggest that pulsed illumination and fluctuating light are distinct photoinhibitory treatments, and different mechanisms protect PSI against them. Full article
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19 pages, 2473 KB  
Article
Evaluating Snow Pavement Strength in Remote Cold Environments via California Bearing Ratio (CBR) and Russian Snow Penetrometer (RSP) Combined Testing
by Katie L. Duggan DiDominic, Margarita Ordaz, Terry D. Melendy and Chrestien M. Charlebois
Glacies 2025, 2(4), 15; https://doi.org/10.3390/glacies2040015 - 4 Dec 2025
Cited by 1 | Viewed by 338
Abstract
Accurate assessment of compacted snow strength is critical for ensuring the safety and performance of snow runways in cold environments. The Russian Snow Penetrometer (RSP) is widely used in snow science and engineering due to its simplicity, portability, and capability for rapid field [...] Read more.
Accurate assessment of compacted snow strength is critical for ensuring the safety and performance of snow runways in cold environments. The Russian Snow Penetrometer (RSP) is widely used in snow science and engineering due to its simplicity, portability, and capability for rapid field measurements under extreme conditions. Conversely, the California Bearing Ratio (CBR) test remains the benchmark for evaluating the load-bearing capacity of conventional granular materials but is seldom applied to snow because of logistical constraints and the material’s complex mechanical behavior. The relationship between these two pavement evaluation tools remains poorly defined. This work investigates how RSP strength indices relate to CBR measurements to determine whether the RSP can serve as a practical proxy for snow pavement load-bearing capacity. Side-by-side field measurements of snow pavement strength were collected over a 30 h period at two test section locations. Both methods captured temporal strength increases and spatial variability, with consistently higher values at the second site attributed to extended sintering. A moderate linear correlation (R2 = 0.44) between RSP and CBR results supports a quantifiable relationship between the two methods. These findings begin to bridge the gap between conventional pavement testing and snow-specific strength evaluation, demonstrating the potential of the RSP for rapid assessment of snow runways. Continued data collection and analysis will refine this relationship and strengthen its applicability for operational use. Full article
(This article belongs to the Special Issue Current Snow Science Research 2025–2026)
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11 pages, 538 KB  
Article
Effects of Acute Red Spinach Powder (VitaSpinach®) Ingestion on Muscular Endurance and Resistance Exercise Performance
by Haley M. Nguyen, Sophia L. Porrill, Rebecca R. Rogers, Josselyn Jose-Gomez, Rachel E. Wright, Phoebe N. Spears and Christopher G. Ballmann
Muscles 2025, 4(4), 60; https://doi.org/10.3390/muscles4040060 - 3 Dec 2025
Viewed by 521
Abstract
Introduction: Red spinach powder (RSP) contains high amounts of inorganic nitrate/nitrite (NO3/NO2), which has been suggested to alter vascular activity, cognitive processing, and sprint exercise performance. There have been few investigations as to whether RSP serves as an ergogenic [...] Read more.
Introduction: Red spinach powder (RSP) contains high amounts of inorganic nitrate/nitrite (NO3/NO2), which has been suggested to alter vascular activity, cognitive processing, and sprint exercise performance. There have been few investigations as to whether RSP serves as an ergogenic aid to improve resistance exercise performance, particularly muscular endurance. Purpose: The purpose of this study was to investigate acute RSP (VitaSpinach®) supplementation on muscular endurance and velocity during bench press exercise. Methods: In a double-blind, counterbalanced crossover manner, resistance-trained males (n = 14) were subjected to two supplement conditions as follows: (1) placebo (PL; purple sweet potato) or (2) red spinach powder (RSP; 400 mg NO3). Supplements were consumed 2 h prior to exercise and blood was collected immediately pre-exercise to determine NO3/NO2 levels. To determine barbell velocity, participants completed two sets × two repetitions with maximal effort, while a rotary encoder measured mean barbell velocity. Following this, participants performed three sets × repetitions to exhaustion (RTE) at 60% of 1-Repetition Maximum (1-RM), separated by 2 min of rest, to determine muscular endurance. Local (lRPE) and global (gRPE) ratings of perceived exertion were measured after exercise. Blood NO3/NO2, RTE, mean velocity, lRPE, and gRPE were compared between supplement conditions. Results: RSP resulted in significantly higher blood levels of total NO3/NO2 (p < 0.001) compared to PL. RSP did not result in superior total RTE (p = 0.935) but increased mean velocity (p = 0.035) compared to PL. Both lRPE (p = 0.027) and gRPE (p = 0.028) were significantly reduced with RSP supplementation. Conclusions: Findings suggest acute RSP ingestion increased NO3/NO2 and bench press velocity. While muscular endurance remained unchanged, RSP resulted in lower perceptions of exertion. Full article
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9 pages, 664 KB  
Opinion
Conservation in High-Field Quantum Transport
by Mukunda P. Das and Frederick Green
Condens. Matter 2025, 10(4), 59; https://doi.org/10.3390/condmat10040059 - 27 Nov 2025
Viewed by 325
Abstract
This article provides an overview of the role of microscopic conservation in charge transport at small scales and at driving fields beyond the linear-response limit. As a practical example, we recall the measurement and theory of interband coupling effects in a quantum point [...] Read more.
This article provides an overview of the role of microscopic conservation in charge transport at small scales and at driving fields beyond the linear-response limit. As a practical example, we recall the measurement and theory of interband coupling effects in a quantum point contact driven far from equilibrium. Full article
(This article belongs to the Special Issue New Advances in Condensed Matter Physics, 2nd Edition)
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15 pages, 2274 KB  
Article
Dimensional Stability of Beech Wood: The Influence of Taper, Slope of Annual Ring and Sawing Pattern
by Peter Vilkovský, Tatiana Vilkovská, Ivan Klement and Martin Fúčela
Polymers 2025, 17(23), 3158; https://doi.org/10.3390/polym17233158 - 27 Nov 2025
Viewed by 355
Abstract
The dimensional stability of sawn timber is one of the key factors affecting processing and final application in various fields, such as construction, furniture making, and interior design. One of the most common problems that beech wood producers may confront is the occurrence [...] Read more.
The dimensional stability of sawn timber is one of the key factors affecting processing and final application in various fields, such as construction, furniture making, and interior design. One of the most common problems that beech wood producers may confront is the occurrence of various types of warping (deformation) during drying. These warps significantly affect the processability of sawn timber, which can lead to reduced yield and economic losses. Several factors can affect dimensional stability. These factors include the sawing pattern, the position of the timber in the log, and the slope of the annual rings. Our research investigated these factors and focused on two types of warping: cup and twist. The results showed a notable influence of the original position of the timber in the log on the degree of cup warping after drying (r = 0.5194; p = 0.0189), with timber closer to the perimeter exhibiting less curvature. The sawing pattern (parallel to the surface of the log—RsP; parallel to the axis of the log—RsO) had a less significant effect but showed a tendency towards curvature (r = 0.4242; p = 0.0623). Based on the sawing pattern, after drying, the twist warping was more pronounced in RsP logs, while RsO cuts retained better shape stability and had only minimal cup warping. Full article
(This article belongs to the Special Issue New Challenges in Wood and Wood-Based Materials, 4th Edition)
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17 pages, 2772 KB  
Article
Spatial Distribution Characteristics and Risk Assessment of Soil Heavy Metals from Long-Term Mining Activities: A Case Study of the Fengfeng Mining Area
by Le Ren, Wenyu Qi and Hongling Ye
Toxics 2025, 13(11), 969; https://doi.org/10.3390/toxics13110969 - 10 Nov 2025
Viewed by 610
Abstract
Long-term mining activities have introduced heavy metals (HMs) into the soil, ultimately threatening environmental sustainability. Precisely forecasting the spatial patterns of HMs and performing risk evaluations in mining regions are essential for efficient pollution control. In this study, 213 topsoil samples were collected [...] Read more.
Long-term mining activities have introduced heavy metals (HMs) into the soil, ultimately threatening environmental sustainability. Precisely forecasting the spatial patterns of HMs and performing risk evaluations in mining regions are essential for efficient pollution control. In this study, 213 topsoil samples were collected from the Fengfeng Mining Area, which has a 150-year mining history. To determine the spatial distribution of soil HM speciation, correlation analysis was conducted by integrating landform types, and visualization was carried out through Kriging interpolation. Results indicate that the mean levels of Cd, Cu, Pb, and Zn exceed their respective background values by 6.48, 1.61, 4.79, and 4.35 times. The bioavailability sequence is Cd > Pb > Zn > Cu, with elevated levels of bioavailable Cd and Pb observed in the western hilly region. Based on the secondary phase to primary phase ratio (RSP) and the risk assessment code (RAC), Pb and Cd were identified as posing high ecological risks, whereas Cu and Zn do not cause severe contamination. This study provides a scientific foundation for industrial transformation and sustainable development in resource-exhausted cities. Full article
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28 pages, 2797 KB  
Article
TTBCA+: An Enhanced Besiege and Conquer Algorithm with Three-Three Tactics and Collision Theory for Complex Optimization Problems
by Jianhua Jiang, Xiangyu Xin, Jun Tian and Hao Li
Electronics 2025, 14(20), 4051; https://doi.org/10.3390/electronics14204051 - 15 Oct 2025
Viewed by 488
Abstract
Besiege and Conquer Algorithm (BCA) is a swarm intelligence algorithm proposed in 2025 based on tactical concepts. However, its besiege and conquer strategies have some problems, such as insufficient diversity and local stagnation. To solve the above problems, this paper proposes an Enhanced [...] Read more.
Besiege and Conquer Algorithm (BCA) is a swarm intelligence algorithm proposed in 2025 based on tactical concepts. However, its besiege and conquer strategies have some problems, such as insufficient diversity and local stagnation. To solve the above problems, this paper proposes an Enhanced Besiege and Conquer Algorithm with Three-Three Tactics and Collision Theory, referred to as TTBCA+. TTBCA+ has four innovations. Firstly, a three-three allocation mechanism of battlefield and roles is proposed to enhance its besiege capability; secondly, a three-three deployment mechanism of soldiers is proposed to enhance its conquer capability; thirdly, the balance factor between exploration and exploitation is modified for three-three tactics implementation; finally, the collision mechanism from collision theory is introduced to deal with soldiers beyond the search space. The performance of the proposed TTBCA+ is verified at the IEEE CEC 2017 and IEEE CEC 2022 benchmark functions, compared with 13 swarm intelligence algorithms, including classical algorithms, well-known algorithms such as JADE, lshade_rsp, AGWO, and recent 5 years algorithms, such as BCA, HOA, PLO, CFOA, HLOA, DBO, BOA. Meanwhile, the proposed algorithms are applied to two practical complex optimization problems. The results show that TTBCA+ effectively solved the limitations in BCA, and it is superior to other compared algorithms. Full article
(This article belongs to the Special Issue AI Applications of Multi-Agent Systems)
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25 pages, 4162 KB  
Article
Spaces, Energy and Shared Resources: New Technologies for Promoting More Inclusive and Sustainable Urban Communities
by Fabrizio Cumo, Elisa Pennacchia, Patrick Maurelli, Flavio Rosa and Claudia Zylka
Energies 2025, 18(16), 4410; https://doi.org/10.3390/en18164410 - 19 Aug 2025
Viewed by 913
Abstract
Renewable Energy Communities (RECs) are central to Europe’s strategy for reducing greenhouse gas emissions and advancing a sustainable, decentralized energy system. RECs aim to transform consumers into prosumers—individuals who both produce and consume energy—thereby enhancing energy efficiency, local autonomy, and citizen engagement. This [...] Read more.
Renewable Energy Communities (RECs) are central to Europe’s strategy for reducing greenhouse gas emissions and advancing a sustainable, decentralized energy system. RECs aim to transform consumers into prosumers—individuals who both produce and consume energy—thereby enhancing energy efficiency, local autonomy, and citizen engagement. This study introduces a novel Geographic Information System (GIS)-based methodology that integrates socio-economic and spatial data to support the design of optimal REC configurations. QGIS 3.40.9 “Batislava” tool is used to simulate site-specific energy distribution scenarios, enabling data-driven planning. By combining a Composite Energy Vulnerability Index (CEVI), Rooftop Solar Potential (RSP), and the distribution of urban gardens (UGs), the approach identifies priority urban zones for intervention. Urban gardens offer multifunctional public spaces that can support renewable infrastructures while fostering local resilience and energy equity. Applied to the city of Rome, the methodology provides a replicable framework to guide REC deployment in vulnerable urban contexts. The results demonstrate that 11 of the 18 highest-priority areas already host urban gardens, highlighting their potential as catalysts for collective PV systems and social engagement. The proposed model advances sustainability objectives by integrating environmental, social, and spatial dimensions—positioning RECs and urban agriculture as synergistic tools for inclusive energy transition and climate change mitigation. Full article
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31 pages, 4078 KB  
Article
A Symmetry-Driven Adaptive Dual-Subpopulation Tree–Seed Algorithm for Complex Optimization with Local Optima Avoidance and Convergence Acceleration
by Hao Li, Jianhua Jiang, Zhixing Ma, Lingna Li, Jiayi Liu, Chenxi Li and Zhenhao Yu
Symmetry 2025, 17(8), 1200; https://doi.org/10.3390/sym17081200 - 28 Jul 2025
Cited by 2 | Viewed by 863
Abstract
The Tree–Seed Algorithm (TSA) is a symmetry-driven metaheuristic algorithm that shows potential for complex optimization problems, but it suffers from local optimum entrapment and slow convergence. To address these limitations, we propose the ADTSA algorithm. First, ADTSA adopts a symmetry-driven dual-layer framework for [...] Read more.
The Tree–Seed Algorithm (TSA) is a symmetry-driven metaheuristic algorithm that shows potential for complex optimization problems, but it suffers from local optimum entrapment and slow convergence. To address these limitations, we propose the ADTSA algorithm. First, ADTSA adopts a symmetry-driven dual-layer framework for seed generation, which promotes effective information exchange between subpopulations and accelerates convergence speed. In later iterations, ADTSA enhances the population’s exploitation ability through a population fusion mechanism, further improving the convergence speed. Moreover, we propose a historical optimal solution archiving and replacement mechanism, along with a t-distribution perturbation mechanism, to enhance the algorithm’s ability to escape local optima. ADTSA also strengthens population diversity and avoids local optima through convex lens symmetric reverse generation based on the optimal solution. With these mechanisms, ADTSA converges more effectively to the global optimum during the evolutionary process. Tests on the IEEE CEC 2014 benchmark functions showed that ADTSA outperformed several top-performing algorithms, such as LSHADE, JADE, LSHADE-RSP, and the latest TSA variants, and it also excelled in comparison with other optimization algorithms, including GWO, PSO, BOA, GA, and RSA, underscoring its robust performance across diverse testing scenarios. The proposed ADTSA’s applicability in solving complex constrained problems was also validated, with the results showing that ADTSA achieved the best solutions for these complex problems. Full article
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19 pages, 2614 KB  
Article
Multiparametric Analysis of PET and Quantitative MRI for Identifying Intratumoral Habitats and Characterizing Trastuzumab-Induced Alterations
by Ameer Mansur, Carlos Gallegos, Andrew Burns, Lily Watts, Seth Lee, Patrick Song, Yun Lu and Anna Sorace
Cancers 2025, 17(15), 2422; https://doi.org/10.3390/cancers17152422 - 22 Jul 2025
Cited by 1 | Viewed by 1108
Abstract
Background/Objectives: This study investigates the utility of multiparametric PET/MRI in delineating changes in physiologically distinct intratumoral habitats during trastuzumab-induced alterations in a preclinical HER2+ breast cancer model. Methods: By integrating diffusion-weighted MRI, dynamic contrast-enhanced MRI, [18F]Fluorodeoxyglucose- and [18F]Fluorothymidine-PET, voxel-wise [...] Read more.
Background/Objectives: This study investigates the utility of multiparametric PET/MRI in delineating changes in physiologically distinct intratumoral habitats during trastuzumab-induced alterations in a preclinical HER2+ breast cancer model. Methods: By integrating diffusion-weighted MRI, dynamic contrast-enhanced MRI, [18F]Fluorodeoxyglucose- and [18F]Fluorothymidine-PET, voxel-wise parametric maps were generated capturing cellular density, vascularity, metabolism, and proliferation. BT-474 tumor-bearing mice have high expression of HER2 and, in response to trastuzumab, an anti-HER2 antibody, effectively show changes in proliferation and tumor microenvironment alterations that result in decreases in tumor volume through time. Results: Single imaging metrics and changes in metrics were incapable of identifying treatment-induced alterations early in the course of therapy (day 4) prior to changes in tumor volume. Hierarchical clustering identified five distinct tumor habitats, which enabled longitudinal assessment of early treatment response. Tumor habitats were defined based on imaging metrics related to biology and categorized as highly vascular (HV), hypoxic responding (HRSP), transitional zone (TZ), active tumor (ATMR) and responding (RSP). The HRSP cluster volume significantly decreased in trastuzumab-treated tumors compared to controls by day 4 (p = 0.015). The volume of ATMR cluster was significantly different at baseline between cohorts (p = 0.03). The TZ cluster, indicative of regions transitioning more to necrosis, significantly decreased in treated tumors (p = 0.031), suggesting regions had already transitioned. Multiparametric image clustering showed a significant positive linear correlation with histological multiparametric mapping, with R2 values of 0.56 (HRSP, p = 0.013, 0.64 (ATMR, p = 0.0055), and 0.49 (responding cluster, p = 0.024), confirming the biological relevance of imaging-derived clusters. Conclusions: These findings highlight the potential utility of multiparametric PET/MRI to capture biological alterations prior to any single imaging metric which has potential for better understanding longitudinal changes in biology, stratifying tumors based on those changes, optimizing therapeutic monitoring and advancing precision oncology. Full article
(This article belongs to the Special Issue Application of Advanced Biomedical Imaging in Cancer Treatment)
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23 pages, 7662 KB  
Article
Comparative Evaluation of the Multispectral Platforms Sentinel-2, CBERS-04A, and UAV for Nitrogen Detection in Maize Crops
by Heloisa Gomes, Gustavo Ferreira da Silva, Juliano Carlos Calonego, Jéssica Pigatto de Queiroz Barcelos, Vicente Marcio Cornago Junior and Fernando Ferrari Putti
AgriEngineering 2025, 7(7), 201; https://doi.org/10.3390/agriengineering7070201 - 20 Jun 2025
Viewed by 1407
Abstract
Multispectral images provide valuable indicators of crop nutritional status, playing a key role in strategies to reduce fertilizer use and enable supplementary applications in cases of nitrogen deficiency, thereby ensuring productivity and profitability for farmers. However, the diversity of remote sensing platforms (RSPs) [...] Read more.
Multispectral images provide valuable indicators of crop nutritional status, playing a key role in strategies to reduce fertilizer use and enable supplementary applications in cases of nitrogen deficiency, thereby ensuring productivity and profitability for farmers. However, the diversity of remote sensing platforms (RSPs) makes the choice challenging, as there are few comparative studies. This study compares the remote sensing platforms Sentinel-2, CBERS-04A, and unmanned aerial vehicle (UAV), assessing their accuracy in detecting different nitrogen doses (NDs) throughout the maize crop cycle in Botucatu-SP, using 10 vegetation indices (VIs). Six NDs were tested (0, 36, 84, 132, 180, and 228 kg ha−1 of nitrogen) in nine assessments during the crop cycle. The results showed that, at the V7 stage, the RSPs were effective in detecting the NDs in eight VIs. However, at the VT stage, only the Sentinel-2 and CBERS-04A satellites demonstrated effectiveness in six VIs. Despite the high correlation among the RSPs, the ability to distinguish the NDs varied depending on the vegetation index (VI) and phenological stage. These findings highlight the importance of selecting the appropriate VI and optimal timing, regardless of the chosen platform. Full article
(This article belongs to the Section Remote Sensing in Agriculture)
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16 pages, 339 KB  
Article
Humoral and T-Cell-Mediated Immunity Against Phlebotomus perniciosus Salivary Proteins in Dogs from a Leishmaniosis-Endemic Area
by Núria Balsells-Aguilar, Maria Magdalena Alcover, Marta Baxarias, Alejandra Álvarez-Fernández, Lourdes Alarcón, Petra Sumova, Petr Volf and Laia Solano-Gallego
Pathogens 2025, 14(6), 576; https://doi.org/10.3390/pathogens14060576 - 10 Jun 2025
Viewed by 1025
Abstract
Compounds in sand fly saliva elicit specific immune responses that may play a role in the establishment of canine Leishmania infection. Although canine antibodies to anti-sand fly saliva antigens have been extensively studied, little is known about cellular immune responses against Phlebotomus perniciosus [...] Read more.
Compounds in sand fly saliva elicit specific immune responses that may play a role in the establishment of canine Leishmania infection. Although canine antibodies to anti-sand fly saliva antigens have been extensively studied, little is known about cellular immune responses against Phlebotomus perniciosus salivary proteins. This study aimed to explore humoral and T-cell-mediated immunity against P. perniciosus salivary proteins in dogs (n = 85) from Mallorca (Spain), a leishmaniosis-endemic area, and find correlations with demographic (age, sex, and breed) and parasite-specific immunological parameters. Anti-sand fly saliva IgG was examined using a P. perniciosus whole salivary gland homogenate (SGH) ELISA and recombinant salivary protein rSP03B ELISA. Interferon gamma (IFN-γ) release whole blood assays with L. infantum soluble antigen (LSA), SGH, and rSP03B were also performed. Positive correlations were found between IgG levels in the SGH and rSP03B tests and between concentrations of SGH IFN-γ and rSP03B IFN-γ. While concentrations of SGH IFN-γ and rSP03B IFN-γ were low and produced only by a minority of dogs (less than 20%), high levels and frequencies of LSA IFN-γ as well as anti-saliva IgG for SGH and rSP03B were detected in a majority of dogs (61% and 75%, respectively). LSA IFN-γ levels were positively correlated with age and Leishmania-specific antibodies. In conclusion, dogs from a leishmaniosis-endemic area presented high humoral immunity against P. perniciosus salivary proteins, but their cellular immunity to these proteins was low and less frequent. Full article
(This article belongs to the Special Issue Pets, Wildlife and Parasites—2nd Edition)
12 pages, 1113 KB  
Article
Identification and Adulteration Evaluation of Rubiae Radix Et Rhizoma and Its Common Adulterants Based on LC-MS and Chemometrics
by Lihui Zhang, Ting Han, Xianrui Wang, Yu Zhang, Jiating Zhang, Wenguang Jing, Minghua Li, Xianlong Cheng and Feng Wei
Molecules 2025, 30(11), 2385; https://doi.org/10.3390/molecules30112385 - 29 May 2025
Cited by 1 | Viewed by 833
Abstract
Background: Rubia schumanniana E. Pritz. (RSP) and Rubia magna P. G. Xiao (RMP) are common adulterants of Rubiae radix et rhizoma (RRR). To realize RRR adulteration analysis, this paper conducted an identification evaluation of RRR based on liquid chromatography–mass spectrometry (LC-MS). Methods: After [...] Read more.
Background: Rubia schumanniana E. Pritz. (RSP) and Rubia magna P. G. Xiao (RMP) are common adulterants of Rubiae radix et rhizoma (RRR). To realize RRR adulteration analysis, this paper conducted an identification evaluation of RRR based on liquid chromatography–mass spectrometry (LC-MS). Methods: After LC-MS analysis and data conversion, the ion intersections were taken from different batches of RSP, RMP, and RRR. Then, the proprietary ions of three herbs were obtained by taking the de-intersection. Finally, the top-n ions were treated as the “ion identity” of RSP, RMP, and RRR for matching test samples to obtain the recognition index (RI) and combined chemometrics for analysis. Results: The identification evaluation of RRR can be realized based on the “ion identity”, even if 3% RSP (or RMP) in RRR samples can still be recognized. Combined with chemometric analysis, three market samples were identified as adulterants, proving that identification evaluation based on “ion identity” is reasonable. Conclusions: The “ion identity” based on LC-MS, which helps in realizing RRR identification evaluation. It has important reference significance for RRR analysis. Full article
(This article belongs to the Special Issue The Application of LC-MS in Pharmaceutical Analysis)
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32 pages, 21417 KB  
Article
Retrievals of Biomass Burning Aerosol and Liquid Cloud Properties from Polarimetric Observations Using Deep Learning Techniques
by Michal Segal Rozenhaimer, Kirk Knobelspiesse, Daniel Miller and Dmitry Batenkov
Remote Sens. 2025, 17(10), 1693; https://doi.org/10.3390/rs17101693 - 12 May 2025
Viewed by 1064
Abstract
Biomass burning (BB) aerosols are the largest source of absorbing aerosols on Earth. Coupled with marine stratocumulus clouds (MSC), their radiative effects are enhanced and can cause cloud property changes (first indirect effect) or cloud burn-off and warm up the atmospheric column (semi-direct [...] Read more.
Biomass burning (BB) aerosols are the largest source of absorbing aerosols on Earth. Coupled with marine stratocumulus clouds (MSC), their radiative effects are enhanced and can cause cloud property changes (first indirect effect) or cloud burn-off and warm up the atmospheric column (semi-direct effect). Nevertheless, the derivation of their quantity and optical properties in the presence of MSC clouds is confounded by the uncertainties in the retrieval of the underlying cloud properties. Therefore, a robust methodology is needed for the coupled retrievals of absorbing aerosol above clouds. Here, we present a new retrieval approach implemented for a Spectro radiometric multi-angle polarimetric airborne platform, the research scanning polarimeter (RSP), during the ORACLES campaign over the Southeast Atlantic Ocean. Our approach transforms the 1D measurements over multiple angles and wavelengths into a 3D image-like input, which is then processed using various deep learning (DL) schemes to yield aerosol single scattering albedos (SSAs), aerosol optical depths (AODs), aerosol effective radii, and aerosol complex refractive indices, together with cloud optical depths (CODs), cloud effective radii and variances. We present a comparison between the different DL approaches, as well as their comparison to existing algorithms. We discover that the Vision Transformer (ViT) scheme, traditionally used by natural language models, is superior to the ResNet convolutional Neural-Network (CNN) approach. We show good validation statistics on synthetic and real airborne data and discuss paths forward for making this approach flexible and readily applicable over multiple platforms. Full article
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