Search Results (401)

Background: Rapid urbanization has intensified the challenges of freight transport, particularly in last-mile (LM) delivery, leading to rising costs and environmental externalities. Autonomous vehicles (AVs) have emerged as a promising innovation to address these issues. While much of the existing literature emphasizes business and operational perspectives, this study focuses on the acceptance of AVs from the standpoint of e-consumers—individuals who make purchases via digital platforms—in an emerging market context. Methods: Grounded in an extended Unified Theory of Acceptance and Use of Technology 2 (UTAUT2), which is specifically suited to consumer-focused technology adoption research, this study incorporates five constructs tailored to AV adoption. Structural Equation Modeling (SEM) was applied to survey data collected from 304 e-consumers in Northeast Brazil. Results: The findings reveal that performance expectancy, hedonic motivation, and environmental awareness exert significant positive effects on acceptance and intention to use AVs for LM delivery. Social influence shows a weaker, yet still positive, impact. Importantly, price sensitivity exhibits a minimal effect, suggesting that while consumers are generally cost-conscious, perceived value may outweigh price concerns in early adoption stages. Conclusions: These results offer valuable insights for policymakers and logistics providers aiming to implement consumer-oriented, cost-effective AV solutions in LM delivery, particularly in emerging economies. The findings emphasize the need for strategies that highlight the practical, emotional, and environmental benefits of AVs to foster market acceptance. Full article

Background/Objectives: Dietary patterns have changed over time, characterising a process of nutritional transition that reflects socioeconomic and demographic inequalities among different populations. This study assessed changes in dietary consumption patterns and the associated social determinants, comparing two time periods in a sample of individuals from a state in the Northeast Region of Brazil. Methods: Data from the 2008–2009 and 2017–2018 Household Budget Survey for the state of Paraíba were analysed, totalling 951 and 1456 individuals, respectively. Foods were categorised according to the NOVA classification and compared based on sociodemographic and economic variables. To determine the factors that most strongly explain the contribution of each NOVA food group to the diet, beta regression analysis was conducted. Results: Differences were observed between the two periods regarding the dietary contribution of the NOVA food groups, with a decrease in consumption of unprocessed foods and an increase in ultra-processed foods. Living in urban areas, being an adolescent, and having an income above the minimum wage were associated with reduced intake of unprocessed foods in both periods. Additionally, being an adolescent and having more than eight years of schooling were associated with higher consumption of ultra-processed foods. Conclusions: The population under study showed changes in food consumption, reflecting a transition process that is occurring unevenly across socioeconomic and demographic groups, thereby reinforcing social inequalities. These findings can guide priorities in food and nutrition policies, highlighting the need for intervention studies to evaluate the effectiveness of such actions. Full article

Glanders is a highly contagious and often fatal zoonotic disease of equids caused by Burkholderia mallei, a pathogen of significant concern due to its potential for bioterrorism. In Brazil, glanders remains endemic, particularly among working equids in the Northeast region. Diagnostic confirmation typically involves serology, culture, and polymerase chain reaction (PCR), although false-negative PCR results have been increasingly reported. This study aimed to evaluate the diagnostic performance and analytical sensitivity of four B. mallei-specific PCR primer sets using samples from 30 seropositive equids. Microbiological cultures were obtained from various organs and swabs, followed by PCR targeting four genomic regions: fliP-IS407A(a), fliP-IS407A(b), Burk457, and Bm17. All animals were confirmed positive for B. mallei via culture, but PCR detection rates varied significantly across primer sets. The fliP-IS407A(b) primer set showed the highest sensitivity, detecting 86% of samples, while the WOAH-recommended fliP-IS407A(a) set had the lowest performance (13.4%). Analytical sensitivity assays confirmed that fliP-IS407A(b) and Bm17 primers detected DNA concentrations as low as 0.007 ng, outperforming the others. These findings suggest that certain widely used primer sets may lack sufficient sensitivity for reliable detection of B. mallei, especially in chronically infected animals with low bacterial loads. The study underscores the need for ongoing validation of molecular diagnostics to improve the detection and control of glanders in endemic regions. Full article

The 13th International Foamy Virus (FV) Conference was held from 8 to 10 November 2023 at the BioParque/Zoological Garden in Rio de Janeiro, Brazil. This was the first conference on spumaretroviruses to be held in the Southern Hemisphere and in the unique environment of the rainforest. New developments and current perspectives in FV research were presented. Highlights of the conference included the structural biology of the envelope protein (Env) and insights into its function and evolution, epidemiologic identification of Amazonian indigenous people with a high prevalence of simian FV (SFV) infections, investigations of virus biology and genomics using synthetic FV DNAs, studies of humoral immune response, and development and applications of SFV vectors. The last day of the meeting was a special tour of the Centro de Primatologia do Rio de Janeiro, located northeast of Rio de Janeiro amidst the protected rainforest, where New World primate hosts of spumaretroviruses are rescued and studied. Our report summarizes the meeting highlights and outcomes for future discussions. Full article

Sepsis is a serious public health problem. sTREM-1 is a marker of inflammatory and infectious processes that has the potential to become a useful tool for predicting the evolution of sepsis. A prediction model for sepsis was constructed by combining sTREM-1, CRP, and a leukogram via a Bayesian network. A translational study carried out with 32 children with congenital heart disease who had undergone surgical correction at a public referral hospital in Northeast Brazil. In the postoperative period, the mean value of sTREM-1 was greater among patients diagnosed with sepsis than among those not diagnosed with sepsis (394.58 pg/mL versus 239.93 pg/mL, p < 0.001). Analysis of the ROC curve for sTREM-1 and sepsis revealed that the area under the curve was 0.761, with a 95% CI (0.587–0.935) and p = 0.013. With the Bayesian model, we found that a 100% probability of sepsis was related to postoperative blood concentrations of CRP above 71 mg/dL, a leukogram above 14,000 cells/μL, and sTREM-1 concentrations above the cutoff point (283.53 pg/mL). The proposed model using the Bayesian network approach with the combination of CRP, leukocyte count, and postoperative sTREM-1 showed promise for the diagnosis of sepsis. Full article

Soil degradation and water scarcity pose major challenges to sustainable agriculture in semiarid regions, requiring innovative strategies to enhance water use efficiency (WUE) and soil fertility. This study assessed the effects of sewage sludge biochar (SSB) on soil properties, WUE, and common bean yield through a small-scale controlled field experiment under rainfed conditions in Northeast Brazil. Four SSB application rates (5, 10, 20, and 40 t ha⁻¹) were compared with conventional NPK fertilization, treated sewage sludge (SS), and chicken manure (CM). The application of 20 t ha⁻¹ (B20) significantly improved soil organic carbon, nitrogen content, water retention, and microbial biomass. B20 also increased WUE by 148% and grain yield by 146% relative to NPK, while maintaining safe levels of potentially toxic elements (PTE) in bean grains. Although 40 t ha⁻¹ (B40) enhanced soil fertility further, it posed a risk of PTE accumulation, reinforcing the advantage of B20 as an optimal and safe dose. These results highlight the potential of SSB to replace or complement conventional fertilizers, especially in sandy soils with limited water retention. The study supports SSB application as a sustainable soil management practice that aligns with circular economy principles, offering a viable solution for improving productivity and environmental resilience in semiarid agriculture. Full article

Staphylococcus aureus (S. aureus) is a major cause of opportunistic infections in humans and animals, leading to severe systemic diseases. The rise of MDR strains associated with animal carriage poses significant health challenges, underscoring the need to investigate animal-derived S. aureus. Objectives: This study examined the genotypic relatedness and phenotypic profiles of antimicrobial resistance in S. aureus, previously sampled from nostril swabs of healthy horses from two geographically distant Brazilian states (Northeast and South), separated by over 3700 km. The study also sought to confirm the presence of methicillin-resistant (MRSA) and borderline oxacillin-resistant (BORSA) strains and to characterize the isolates through molecular typing using PCR. Methods: Among 123 screened staphylococci, 21 isolates were confirmed as S. aureus via biochemical tests and PCR targeting species-specific genes (femA, nuc, coa). Results: REP-PCR analysis generated genotypic profiles, revealing four antimicrobial resistance patterns, with MDR observed in ten isolates. Six isolates exhibited cefoxitin resistance, suggesting methicillin resistance, despite the absence of the mecA gene. REP-PCR demonstrated high discriminatory power, grouping the isolates into five major clusters. Conclusions: The genotyping indicated no clustering by geographical origin, highlighting significant genetic diversity among S. aureus strains colonizing horses’ nostrils in Brazil. These findings highlight the widespread and varied nature of S. aureus among horses, contributing to a deeper understanding of its epidemiology and resistance profiles in animals across diverse regions. Ultimately, this genetic diversity can pose a public health risk that the epidemiological surveillance services must investigate. Full article

This study aimed to investigate food insecurity (FI) and its association with sociodemographic characteristics and behavioral and dental alterations in adolescents from a county in the inland of northeastern Brazil. Data on 192 adolescents aged 11–14 years were analyzed in the public school system in Juripiranga, Paraíba, Northeast Brazil. The adolescents and their guardians responded to the Brazilian Food Insecurity Scale, the Strength and Difficulties Questionnaire for the caregiver, the Strength and Difficulties Questionnaire for children and adolescents, and a sociodemographic questionnaire. Academic performance was observed by calculating the median of the final assessments of basic school subjects. The prevalence of FI was 69.19, and FI was associated with a family income of up to one minimum wage per month (prevalence ratio [PR]: 1.90; 95% confidence interval [95% CI]: 1.20–3.01), no practice of religion by the guardian (PR: 1.34; 95% CI: 1.04–1.73), behavior considered inappropriate by the guardian (PR: 1.33; 95% CI: 1.02–1.73), and academic performance (PR: 1.35; 95% CI: 1.05–1.72). FI is considered a complex and multifactorial problem that requires appropriate intervention to deal with multiple social determinants. The results point to the need for integrated public policies between the health, education, social assistance, and food security sectors. Full article

The Brazilian semi-arid Northeast plays a critical role in regional hydrology, where rainfall is marked by pronounced temporal variability and short duration, presenting significant challenges for understanding and managing hydrological and erosive processes. This study aims to evaluate the performance of empirical models for estimating rainfall kinetic energy (KE) and erosivity index (EI₃₀) in this region, for all events and erosive events, using high-resolution rainfall data collected at the Federal University of Cariri (UFCA), Ceará. A total of 283 natural rainfall events were analyzed, with KE and EI₃₀ values calculated using multiple models: Wischmeier and Smith, USDA, Van Dijk, a temporal variation-based model (KE_VT), and a regional model developed for Brazil’s semi-arid zone, which served as the reference. The results show a predominance of small rainfall events (<5.2 mm), though maximum EI₃₀ values exceeded 1300 MJ ha⁻¹ mm h⁻¹, highlighting the potential for extreme erosive events. Comparative analysis revealed that all international models significantly underestimated KE and EI₃₀ values compared to the regional reference, with the KE_VT model showing the closest approximation (13% underestimation), for all events and erosive events. Statistical assessments using the Wilcoxon test, Nash–Sutcliffe efficiency, and Willmott concordance index confirmed the superior performance of the KE_VT, for all events and erosive events. These findings underscore the importance of considering intra-event rainfall variability and regional calibration when modeling erosivity in semi-arid climates, contributing to more effective soil conservation and hydrological planning. Full article

In this study, flotation tests were conducted on a laboratory scale using a sample of microcrystalline graphite ore from the Canindé region, Ceará, Brazil. The objective was to investigate the grinding time, reagent dosage, and purification process for obtaining graphene-based nanomaterials. Natural graphite has a stacked planar structure and exhibits polymorphism with rhombohedral, hexagonal, and turbostratic geometries, characteristics that directly influence its properties and technological applications. The results demonstrated that it was possible to obtain rougher concentrate with a graphite carbon content of 23.4% and a recovery of 86.4%, using a grinding time of 7.5 min and reagent dosages of 150 g/t of kerosene and 100 g/t of Flotanol D-25. This flotation process resulted in a graphite concentrate with 76.6% graphite carbon content. To increase the purity of the concentrate and expand its industrial applications, the graphite was purified in an alkaline autoclave using the hydrothermal method. In the next stage, acid leaching was performed, and this chemical treatment destabilized the regular stacking of the graphite layers, promoting the formation of graphene-like nanoplates, including monolayer graphene. Thus, the nanomaterials obtained through the process developed in this study have potential for various innovative applications, such as lithium-ion batteries, electric vehicles, and two-dimensional graphene-based materials. Full article

This study aimed to identify spatial clustering and maternal and birth-related factors associated with the incidence of orofacial clefts in Brazil from 2001 to 2022. A nationwide ecological study was conducted in Brazil using data from 2001 to 2022 obtained from the Brazilian Live Birth Information System (SINASC). The municipality was used as the spatial unit of analysis. Variables included maternal age and education, newborn sex, gestational age, birth weight, and skin color/ethnicity. Univariate and bivariate global and local Moran’s I indices were used to assess spatial autocorrelation. A total of 234 municipalities (4.2%) formed high–high spatial clusters, primarily in the South and Southeast, while 431 municipalities (7.7%) formed low–low clusters, mostly in the Northeast (Moran’s I = 0.121, 95% CI: 0.107 to 0.135). High–high clusters had a lower median proportion of adolescent mothers (≤19 years: 17.4%) and a higher proportion of mothers aged ≥ 35 years (12.9%) compared to low–low clusters (23.5% and 8.7%, respectively; p < 0.001). High–high clusters also had fewer mothers with less than seven years of education (31.0% vs. 45.9%, p < 0.001) and higher rates of preterm births and low birth weight (p < 0.001). The proportion of White newborns was higher in high–high clusters than in low–low clusters (82.8% vs. 13.6%, p < 0.001). These findings suggest that orofacial cleft incidence in Brazil is spatially associated with maternal sociodemographic characteristics, perinatal outcomes, and newborn race/ethnicity. Full article

The affected family members (AFM) of relatives with substance use problems (RSU) play an important role in supporting their relatives to enter substance use treatment. This study investigated the help-seeking behaviours for their relatives by AFM in Brazil, including the characteristics of those who sought help and the risk factors for delaying it. A secondary analysis from a national cross-sectional study of 3030 AFM was performed. Participants were recruited from a range of services focused on AFM across each of the five Brazilian regions (North, Northeast, Central-West, Southeast, South). While 92.7% sought help, 66.0% delayed for an average of 37.2 (SD 70.71) months. Help seeking was associated with higher socioeconomic status and being from the Southeastern region. Barriers included the relative refusing help (31.5%) and the belief that help was not needed (20.6%). Longer delays were associated with female AFM, residents in the Central-West region, non-parents, older RSU, alcohol use, and withdrawal coping strategies. The findings show disparities in help-seeking behaviour across socioeconomic groups, regions, and substance types, highlighting the need for better healthcare workforce distribution and targeted interventions to educate AFMs on the importance of engagement with healthcare services. Full article

The Lutjanidae family includes multiple species highly important to the global fishing industry. In Brazil, approximately 40% of the fishing landings come from a species of this family, the dog snapper, Lutjanus jocu, among the most abundant in the northeast-region fisheries. This study aimed to analyze the genetic diversity and population structure of this species in the states of Bahia and Espírito Santo through the use of microsatellite markers. The dog snapper presented a high genetic variability in the studied populations, with the presence of a distinct population stock in northern Bahia probably driven by habitat suitability, larvae retention, and fishing pressure. The L. jocu sampling sites exhibited an excess of heterozygosity, a low allelic richness, and M-ratio values close to critical levels, probably indicating a recent population decline. Additionally, the low inbreeding indices and high genetic diversity values suggest a significant connectivity and considerably effective population sizes. Although these characteristics may reflect population stability, anthropogenic factors such as habitat loss, fragmentation, and overfishing may pose threats to the sustainability of the species, particularly along the northeastern coast of Brazil. Full article

In semi-arid regions, seasonally dry tropical forests are essential for regulating the surface energy balance, which can be analyzed by examining air heating processes and water availability control. The objective of this study was to evaluate the ability of the Brazilian Developments on the Regional Atmospheric Modelling System (BRAMS) model in simulating the seasonal variations of the energy balance components of the Caatinga biome. The surface measurements of meteorological variables, including air temperature and relative humidity, were also examined. To validate the model, we used data collected in situ using an eddy covariance system. In this work, we used the BRAMS model version 5.3 associated with the Joint UK Land Environment Simulator (JULES) version 3.0. The model satisfactorily represented the rainfall regime over the northeast region of Brazil (NEB) during the wet period. In the dry period, however, the coastal rainfall pattern over the NEB region was underestimated. In addition, the results showed that the surface fluxes linked to the energy balance in the Caatinga were impacted by the effects of rainfall seasonality in the region. The assessment of the BRAMS model’s performance demonstrated that it is a reliable tool for studying the dynamics of the dry forest in the region, providing valuable support for sustainable management and conservation efforts. Full article

In the eastern Brazilian Amazon, the Viseu municipality has almost 70% of its territory deforested. Monitoring viruses from wildlife hosts enables the prevention and control of epizootic events and outbreaks. Seven samples from three marsupials and two rodents were screened by high-throughput sequencing for virome analysis. The three samples from the two Proechimys roberti rodents, one from the liver, one from the brain, and one from a pooled viscera sample, showed the highest results in terms of viral abundance and richness. From these we obtained two strains of a new rodent hepacivirus (RHV), which belongs to a new putative genotype of an unclassified RHV species previously described in Panama and Northeast Brazil. The findings expand the host range of the cited RHV species, imply virus circulation in the study area, and suggest a viral tropism in the liver and perhaps in the brain. Full article