Search Results (65)

Ruminants significantly contribute to global methane (CH₄) emissions, necessitating the development of dietary mitigation strategies. This study evaluated five Bangladeshi seaweeds (brown, red, and green types) from Saint Martin Island for their anti-methanogenic potential through phenotypic identification, proximate analysis, and in vitro fermentation assessment. Significant interspecies variation was (p < 0.001) observed in dry matter (DM: 82.1–99.9%), acid detergent fiber (ADF: 17.4–24.9%), neutral detergent fiber (NDF: 29.6–43.6%), and dry matter degradability (DMD: 43.9–58.7%), while crude protein (CP) remained consistent (p = 0.574). After 48 h of fermentation, total gas (1.3–22.1 mL/g DM) and CH₄ yield (0.04–1.6 mL/g DM) varied markedly (p < 0.01). DMD was strongly correlated with total gas and CH₄ production. Crucially, both ADF and NDF showed a positive correlation with total gas and CH₄ production. However, NDF displayed a weak positive correlation with DMD. These findings suggest atypical fiber fraction dynamics, contrasting with terrestrial forages. Supplementation effects of two red seaweeds, SW-4 (Gracilaria parvispora) and SW-5 (Asparagopsis taxiformis), on Napier grass were assessed at 5% and 10% inclusion levels. SW-5 reduced CH₄ by 52.7% when co-fermented with Napier grass at a 10% inclusion level for 48 h, whereas SW-4 showed no significant effect. These results highlight SW-5 as a promising dietary supplement to reduce enteric CH₄ in ruminants, suggesting further in vivo validation for optimal use. Full article

Conjugated linoleic acid (CLA) is recognized as a bioactive compound with significant health-promoting properties. This study aimed to evaluate the potential of palm oil derivatives to enhance milk CLA levels and milk fatty acid quality in Holstein Friesian dairy cows. The study was conducted using a randomized block design (RBD) with four treatments and four replications: (1) Basal ration (Napier grass + concentrate); (2) Basal ration + 2.2% palm-calcium soap (PCS); (3) Basal ration + 2% palm-prilled fat (PPF); and (4) Basal ration + 2% hard palm stearin (HPS). The results showed that palm-prilled fat (PPF) supplementation in the dairy cow significantly improved (p < 0.05) milk fatty acid profile (C18:1-cis and Conjugated Linoleic Acid) and decreased (p < 0.05) ΣtFA in milk. Palm-prilled fat supplementation significantly increased (p < 0.05) the C14:1 and C18:1 desaturase index (DI) and enhanced the health-promoting index (HPI) in milk. Based on this study, it can be concluded that supplementation with PPF was able to enhance milk quality. Furthermore, dietary fat supplementation led to an improved milk fatty acid profile, which may increase its value as a functional food and promote human health. Full article

Silage is a core roughage resource for ruminant production, but aerobic deterioration caused by microorganisms severely reduces its nutritional value and increases microbial risk. This study aimed to isolate and identify specific spoilage organisms (SSOs) from Napier grass silages during aerobic deterioration and evaluate their spoilage capability. Based on morphological observation, physiological and biochemical tests, and ITS rDNA sequence analysis, four SSOs were obtained as Trichosporon asahii (TA32), Nakaseomyces glabratus (NG38), Candida tropicalis (CT39), and Pichia kudriavzevii (PK41) with high lactate-assimilating and spoilage capacity. All four strains were facultative anaerobic yeast and exhibited robust growth within the range of 25–40 °C and pH 3.5–6.5. To verify their spoilage capability, these purified strains were inoculated into Napier grass silage and exposed to air. Fermentation and chemical parameters were monitored at 0, 2, 5, and 9 days. Results showed that silages inoculated with PK41 or TA32 exhibited the lowest aerobic stability with most rapid increase in pH (p < 0.05), while the control (CON) remained the highest aerobic stability (p < 0.05). These results provide a theoretical basis for developing targeted preservation technologies to extend the shelf-life of silage. Full article

This study investigated the effects of soybean meal on fermentation characteristics, nutritional composition, bacterial community, and functional metabolic prediction in Napier grass silage. Napier grass was treated with soybean meal at 0% (CK), 10% (SA), 15% (SB), and 20% (SC) and ensiled for 7, 15, 30, 60, and 90 days. After 90 days of ensiling, SA, SB, and SC significantly increased (p < 0.05) lactic acid (LA) concentration, acetic acid (AA), the ratio of LA/AA, dry matter (DM), and crude protein (CP), and pH level while decreasing butyric acid (BA), ethanol, NH₃-N, NDF, ADF, and ADL compared to CK silage. At 7 days of ensiling, the SA, SB, and SC treatments increased the abundance of Leuconostoc, Pediococcus, and Klebsiella compared to the control. after 30 days of fermentation, the dominant genus shifted to Lactococcus in the SA, SB, and SC treatments, which was accompanied by a higher abundance of Klebsiella. In contrast, Lactobacillus became the dominant genus in the CK silage. Spearman’s rank correlation analysis revealed a positive correlation between DM content and Lactococcus and a negative correlation with NH₃-N concentration. pH and DM correlate negatively with Lactobacillus. LA, propionic acid, and AA show a negative correlation with Weissella. Enterobacter positively correlates with PA concentration. These findings demonstrate that SA improves the fermentation quality, and SB and SC could improve the nutritional content and microbial diversity abundance. We recommended ensiling Napier grass silage with SB (15%) doses and the best ensiling duration is 60 days. Full article

Napier grass (Cenchrus purpureus Schumach) is an important forage crop and livestock feed. However, its yield and quality in Kenya are often limited by Napier grass headsmut and stunt disease. Napier grass genetic improvements through mutation breeding and selection could avail cultivars with increased forage. This study investigated the response of embryogenic calli to different levels of colchicine in inducing polyploidy in the two germplasms of Napier grass; South africa and Bana grass. The experiments were carried out as a factorial experiment in a completely randomized design (CRD). The colchicine concentrations used were 0, 0.05, 0.1, and 0.2%, and the exposure durations were 24, 48, and 72 h. During the shoot regeneration stage, culturing explants on an MS medium (Murashige and Skoog) supplemented with 0.2 mg L⁻¹ Benzyl Adenine (BAP), 0.1 mg L⁻¹ dichlorophenoxyacetic acid (2, 4-D), and 0.1 mg L⁻¹ indole-3-butyric acid (IBA) was more suitable for shoot regeneration. Chromosome doubling was confirmed by genomic DNA and the stomata size and number. Culturing explants on an MS medium supplemented with 1 mg L⁻¹ IBA, 1 mg L⁻¹ 2, 4-D, and 0.5 mg L⁻¹ BAP was more suitable in inducing embryogenic calli in both genotypes. Polyploidy results revealed that a 0.1% concentration of colchicine with two days of treatment established the maximum number of octoploid plantlets induced in vitro, while a 0.2% concentration was very toxic. The stomata size and number of derived octoploid plantlets were bigger with a lower density, a shorter plant height, and a smaller stem diameter, and despite being the first to produce tillers, they were significantly higher than their progenitors. Induced mutants also had a significantly higher number of chromosomes and showed different band patterns and distances during gel electrophoresis. However, we recommend the use of flow cytometry to confirm the ploidy level. The superior mutant plantlets can be selected and recommended for characterization across representative agro-ecologies for large-scale production and used in Cenchrus purpureus breeding programs in Kenya and its environments. Full article

In this study, the effects of composting phase and bulking agent on macronutrient extraction and the chemical, physicochemical, and biological properties of 20 compost teas from poultry waste composting mixtures were evaluated. Phosphorus (P) extraction was more efficient during stabilization after the thermophilic phase; however, water-soluble P declined as composting progressed. K was more amenable to extraction, with yields ranging from 30% to 70%, followed by N (2% to 12%) and P (1% to 7%). Compost tea quality was clearly affected by both the bulking agent and the composting stage. Bulking agents that accelerate the process, such as cotton waste (CW) and Napier grass (NG), contributed to nutrient mineralization, increasing availability in the compost tea but also raising salt contents responsible for phytotoxicity. In contrast, tree trimmings (TT), sawdust (S), and sugarcane bagasse (SCB) showed better results, striking a balance between nutrient availability and salt content. The period between the thermophilic phase and cooling was the most suitable for extraction, providing the greatest contribution of water-soluble nutrients. This study highlights the influence of bulking agents and composting phases on nutrient extraction and phytotoxicity of compost teas and provides new insights into the role of electrical conductivity as a threshold indicator for safe agricultural application. Full article

Tropical forage crops vary widely in biochemical composition, resulting in inconsistent silage quality. Understanding how plant traits shape microbial and metabolic networks during ensiling is crucial for optimizing fermentation outcomes. Eight tropical forages—Sorghum bicolor (sweet sorghum), Sorghum × drummondii (sorghum–Sudangrass hybrid), Sorghum sudanense (Sudangrass), Pennisetum giganteum (giant Napier grass), Pennisetum purpureum cv. Purple (purple elephant grass), Pennisetum sinese (king grass), Leymus chinensis (sheep grass), and Zea mexicana (Mexican teosinte)—were ensiled under uniform conditions. Fermentation quality, bacterial and fungal communities (16S rRNA and ITS sequencing), and metabolite profiles (untargeted liquid chromatography–mass spectrometry, LC-MS) were analyzed after 60 days. Sweet sorghum and giant Napier grass showed optimal fermentation, with high lactic acid levels (111.2 g/kg and 99.4 g/kg, respectively), low NH₄⁺-N (2.4 g/kg and 3.1 g/kg), and dominant Lactiplantibacillus plantarum. In contrast, sheep grass and Mexican teosinte exhibited poor fermentation, with high NH₄⁺-N (6.7 and 6.1 g/kg) and Clostridium dominance. Fungal communities were dominated by Kazachstania humilis (>95%), while spoilage-associated genera such as Cladosporium, Fusarium, and Termitomyces proliferated in poorly fermented silages. Metabolomic analysis identified 15,827 features, with >3000 significantly differential metabolites between silages. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed divergence in flavonoid biosynthesis, lipid metabolism, and amino acid pathways. In the sweet sorghum vs. sheep grass comparison, oxidative stress markers ((±) 9-HODE, Agrimonolide) were elevated in sheep grass, while sweet sorghum accumulated antioxidants like Vitamin D3. Giant Napier grass exhibited higher levels of antimicrobial flavonoids (e.g., Apigenin) than king grass, despite both being dominated by lactic acid bacteria. Sorghum–Sudangrass hybrid silage showed enrichment of lignan and flavonoid derivatives, while Mexican teosinte accumulated hormone-like compounds (Gibberellin A53, Pterostilbene), suggesting microbial dysbiosis. These findings indicate that silage fermentation outcomes are primarily driven by forage-intrinsic traits. A “forage–microbiota–metabolite” framework was proposed to explain how plant-specific properties regulate microbial assembly and metabolic output. These insights can guide forage selection and development of precision inoculant for high-quality tropical silage. Full article

Plant secondary metabolites regulate plant growth and serve as valuable pharmaceutical resources. Napier grass (Pennisetum purpureum Schumach.), a Poaceae species, shows potential as a functional food. In this study, we employed high-resolution mass spectrometry combined with a data-independent acquisition (DIA) strategy for the untargeted detection of anthocyanins, a group of secondary metabolites, in napier grass. Clear MS² fragmentation patterns were observed for anthocyanins, characterized by diagnostic aglycone signals and sequential losses of hexosyl (C₆H₁₀O₅), deoxyhexosyl (C₆H₁₀O₄), pentosyl (C₅H₈O₄), and p-coumaroyl groups (C₉H₈O₃). Based on matching with authentic standards and an in-house database, ten anthocyanins were identified, seven of which were newly reported in napier grass. In a single-laboratory validation analysis, both absolute and semi-quantitative results reliably reflected the specific distribution of metabolites across different cultivars and plant organs. The purple cultivar (TS5) exhibited the highest anthocyanin content, with the cyanidin 3-O-glucoside content reaching 5.0 ± 0.5 mg/g, whereas the green cultivar (TS2), despite its less pigmented appearance, contained substantial amounts of malvidin 3-O-arabinoside (0.7 ± <0.1 mg/g). Flavonoid profiling revealed that monoglycosylated anthocyanins were the dominant forms in floral tissues. These findings shed light on napier grass metabolism and support future Poaceae breeding and functional food development. Full article

Agricultural byproducts, including pineapple peel (PP), are valuable feed additives which support the livestock industry. However, conflicting evidence exists regarding the optimal amount of PP required to achieve optimal fermentation in silage. This study examines the impact of ensiling mixtures of equal proportions of fresh Napier grass (NG) and sugarcane top (ST) with varying levels of PP (0% [C], 10% [P1], 20% [P2], and 30% [P3]) on fermentation quality, microbiological profiles, and in vitro ruminal digestion. Compared to the C silage, the dry matter, crude protein, and neutral detergent fiber contents decreased in the silage treated with increasing PP (p < 0.05). P1 exhibited lower (p > 0.05) pH, higher (p > 0.05) lactic acid content, and lower (p < 0.05) NH₃-N content than other silage. The Chao 1, ACE index, and relative abundance of Lacticaseibacillus and Lactobacillales were decreased following the order of C > P1 > P2 > P3 (p < 0.05). Although there were no significant differences observed in most vitro ruminal fermentation parameters among four silages (p > 0.05), P1 exhibited higher total gas production, total volatile fatty acid, acetate acid, acetate-to-propionate ratio, and lower pH than the other silages. These results demonstrated that a NG and ST mixture co-ensiling with appropriate PP enhances the NG and ST mixture silage quality, and the optimum addition ratio for PP was 10% on a fresh matter basis. Full article

Napier grass is widely used across the tropics and subtropics as a feed for dairy and beef cattle, but its quality is poor under current management with resultant poor animal productivity. Current management is focused on achieving high yields and, as such, is harvested at ground level with longer harvest interval when grass reaches 200 cm or higher, which reduces quality. The opportunity to improve the nutritive value of this grass is largely unexplored alongside the opportunity to increase livestock productivity and food security in the tropics and subtropics. Here we determined the impact of leaf number (total) or stage (LS; 6, 9 and 14) at harvest, and harvest severity height (SH; cutting or harvest height from the ground; 5, 10 and 20 cm), on the nutritive value of Napier grass (cv. Pakchong) across one year. Napier grass was sown (from cutting) at a density of 50 cm × 50 cm with three replicates per treatment. Increasing LS from 6 to 14 decreased crude protein (CP) content from 184 g/kg DM to 118 g/kg DM and metabolizable energy (ME) content from 10.4 to 7.3 MJ/kg DM. These results suggest that Napier grass should be harvested at a lower LS to increase plant nutritive value but there was a trade-off between yield and quality as yield decreased by half to improve such quality. The impact of offering Napier grass harvested at 6, 9 or 14 LS on cattle growth was then determined across 113 days in a second experiment. Red Chittagong bulls were enrolled at a weight of a 181.9 ± 2.30 (Mean ± SE) Kg with an age of between 18 and 22 months. The 6 LS treatment cattle had a greater growth rate and higher feed conversion ratio (FCR) than the 14 LS treatment (610 versus 270 g/day and 6.4 versus 16.2, respectively) indicating a trade-off of yield with quality. Our results demonstrate that Napier grass should be harvested at 6 LS with 10 cm SH for feed nutritive value, cattle growth and FCR. Further research is required to ameliorate yield loss associated with this recommended management strategy with a focus on increasing plant density. Full article

Insufficient access to high-quality feed in sufficient amounts is hindering the sustainable growth of the Indian cattle sector. The feed supply is negatively impacted by increased cropping intensity, limited grazing land, and the effects of climate change. Therefore, developing cost-effective methods to improve feed availability year-round is crucial. Improved planted forages, such as Napier grass, are recommended to address feed shortages in semi-arid agroecological regions in India. The study, using the PSM approach, investigates the socioeconomic factors that impact Napier adoption, its influence on enhanced milk output, time saved in livestock farming, farmers’ well-being, and livestock health. This study employed a multistage sampling method to choose 309 participants for the questionnaire survey. Our analysis shows that Napier adoption resulted in a 24.6% rise in daily milk output/cow and a 61.2% overall improvement in total milk production/year/cow when compared with baseline data. Napier’s adoption decreased livestock farming times by 30 min/cow. Additionally, women’s involvement in livestock farming improved with Napier farming, and farmers who have switched to Napier have seen a remarkable increase in their net income, with a monthly boost of Rs. 2044–2555 per cow. Additionally, daily milk consumption has also skyrocketed, with a remarkable enhancement of 143–153 mL per person daily. Our study highlights that the farmer’s age, education level, livestock unit, and land holding play a crucial role. Additionally, the availability of extension services and farmer group participation can further impact the adoption process. Furthermore, our study explores how these factors shape the decision-making process and drive the successful integration of Napier grass into farming practices. However, considering the spatial limitations and reliance on self-reported data in this study, we suggest future research examining the long-term effects of Napier grass adoption on climate-smart agricultural practices, soil moisture, and socioeconomic benefits, involving field experiments, modeling, and farmer participation. Full article

Food grain production has multiplied over the last two decades in India, but natural resources are overexploited in modern farming. Farmers, especially those with small and marginal holdings, are suffering losses more often than not, the cost of production is increasing year after year, and profits are not up to the necessary levels. To address such challenges, there has been a broad recognition of the importance of employing farming system approaches in research. The cultivation of cropping systems with orchard crops and livestock components can play a significant role in the optimal utilization of resources, enhancing energy use efficiency as well as the eco-efficiency index, and reducing carbon footprints. This study was carried out to create a suitable IFS model with high economic and energy efficiency for small-holder farmers in India’s southern plateau and hills with a negligible impact on the environment. The following were the seven models: M₁: Rice − Groundnut; M₂: Rice − Groundnut, Pigeonpea + Sweetcorn (1:3) − Bajra, Bt cotton + Greengram (1:2) − Maize; M₃: Rice − Groundnut, Pigeonpea + Sweetcorn (1:3) − Bajra, Pigeonpea + Maize (1:3) − Sunhemp; Napier grass, Sheep (5 + 1); M₄: Rice − Groundnut, Pigeonpea + Sweetcorn (1:3) − Bajra, Bt cotton + Greengram (1:2) − Maize, Pigeonpea + Maize (1:3) − Sunhemp, Poultry unit; M₅: Guava, Hedge Lucerne, Napier grass, Bt cotton + Greengram (1:2) − Maize, Sheep (5 + 1); M₆: Guava, Bt cotton + Greengram (1:2) − Maize, Rice − Groundnut, Poultry; M₇: Rice − Groundnut, Pigeonpea + Sweetcorn (1:3) − Bajra, Pigeonpea + Maize (1:3) − Sunhemp; Napier grass, Hedge lucerne, Poultry (100), Sheep (5 + 1). Model M₁ was used to represent the local region, and the other models were compared in terms of economics, energetics, greenhouse gas emissions, and employment creation. The M₇ and M₃ models, according to the results, have higher economic efficiency (₹342.3 day⁻¹, ₹263.7 day⁻¹), increase output energy (228,529 and 183,231 MJ) net energy (258,184 and 198,920 MJ), produce net negative emissions (−2842 and −2399 kg CO₂ eq.), and create jobs year-round (112.5 and 110.5 man days year⁻¹), respectively. This is primarily because they have multiple highly efficient components that make them viable for Telangana’s small and marginal farmers. Full article

The fall armyworm (FAW), Spodoptera frugiperda, is a major polyphagous pest that mainly feeds on maize and other cash crops. Understanding S. frugiperda’s behavior on different host plants facilitates the development of effective integrated pest management (IPM) plans. Therefore, this study investigated the oviposition preferences of S. frugiperda females among different host plants using no-choice, two-choice, and multiple-choice bioassays. In no-choice bioassays, para grass, Urochloa mutica (Forssk.) (Poales: Poaceae); maize, Zea mays (L.) (Poales: Poaceae); and napier grass, Pennisetum purpureum (Schumach) (Poales: Poaceae) were identified as highly attractive, while sweet sorghum, Sorghum dochna (Forssk.) (Poales: Poaceae); sunhemp, Crotalaria juncea (L.) (Fabales:Fabacea); Egyptian clover, Trifolium alexandrinum (L.) (Fabales:Fabacea); desmodium, Desmodium uncinatum (Jacq.) (Fabales:Fabacea); natal grass, melinis repens (Zizka) (Poales: Poaceae); molasses grass, Melinis minutiflora (P.Beauv.) (Poales: Poaceae); and mung bean, Vigna radiata (R. wilczek) (Fabales: Fabaceae) exhibited reduced oviposition effects. Two-choice bioassays revealed different levels of attractiveness and repellency among different plant combinations. In multiple-choice bioassays, mimicking an intercropping scenario, differences in the number of eggs and egg mass were observed for M:S:D:W (maize, sunhemp, desmodium, and cage wall), S:D:M:W (sunhemp, desmodium, maize, and cage wall), and D:M:S:W (desmodium, maize, sunhemp, and cage wall). This study provides insights into the egg-laying preferences of S. frugiperda females among different host plants, valuable for the management of S. frugiperda. This encourages further research and further identification of novel repellent and attractant host plants, which will ultimately contribute to the development of sustainable and environmentally friendly crop production practices and techniques. Full article

In this study, our subjects were 18 female crossbred Saanen lactating goats; the goats weighed approximately 43.25 ± 2.35 kg and had been producing milk for 14 ± 2 days. They were assigned to three equally sized experimental groups based on the cycle of the lactation period. In accordance with the randomized complete block design (RCBD), six animals were randomly assigned to the treatments in each block. There were three treatments: (1) control = Napier Pakchong-1 grass silage (NPS); (2) = sweet grass silage (SGS); and (3) = purple Napier silage (PNS). Our findings show that the goats fed with purple Napier grass silage had a higher DM intake (1805.97 g/day) than those that underwent a different treatment (1312.76 and 1443.43 g/day). The goats fed with purple Napier grass silage had higher protein digestibility (79.85) than those fed with Napier Pakchong-1 and sweet grass silage. There were significant differences in the ruminal ammonia nitrogen and blood urea nitrogen. Nevertheless, purple Napier grass silage significantly increased butyric acid 2 and 4 h after feeding and increased the number of microorganisms. In the dairy goats fed purple Napier grass silage, we observed a reduction in protozoa and methanogen populations. The levels of antioxidants shown by the plasma indices differed in the goats fed with purple Napier grass silage and showed enhanced milk composition, higher levels of total antioxidants, superoxide dismutase and glutathione peroxidase in the plasma, reduced protozoa methanogen, and a decreased level of malondialdehyde in the plasma. This study highlights that purple Napier grass silage has the potential to be a beneficial source of roughage for lactating dairy goats. Full article

The co-ensiling technique is widely used to improve silage quality; however, it remains unclear as to what high-quality silages can be made by co-ensiling Napier grass (NG) with Sugarcane top (ST). The aim of this study was to evaluate the fermentation characteristics, chemical composition, and microbiological profile of silage produced from mixtures of NG and ST in varying ratios. Silage was prepared using a small-scale fermentation system, and treatments were designed as control silage (NG ensiled alone) or with 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%ST on a fresh matter basis with six replicates, respectively. Increasing ST in the silage reduced the contents of crude protein, ash, acetic acid, butyric acid, ammonia-N, as well as pH, but increased the contents of dry matter, ether extract, neutral detergent fiber, acid detergent fiber, water-soluble carbohydrate, lactic acid, and lactic acid bacteria. Lactobacillales and Enterobacterales were the dominant orders, with Lactiplantibacillus and Weissella as the dominant genera. Co-ensiling NG with ST enhanced microbial diversity and richness. ST, as a local by-product, is a viable additive to improve NG silage quality and nutrition. This study suggests that good-quality silages can be produced with NG: ST ratios of 40:60 to 20:80 and that these silages offer an opportunity to optimize the nutrient supply for ruminants. Full article