Search Results (89)

The global water crisis represents one of humanity’s most pressing challenges, with over 2 billion people lacking access to safely managed drinking water. This study presents the implementation and evaluation of an innovative air-conditioning condensate recovery system at Building F of the Faculty of Electrical and Electronic Engineering (FIEE), Universidad Veracruzana, Mexico. The system integrates twenty-six 24,000 BTU air-conditioning units across twelve classrooms and two laboratories, recovering approximately 520 L of condensate water daily. An initial physicochemical characterization of the recovered condensate was conducted through pH, electrical conductivity (EC), and total dissolved solids (TDS) measurements. In addition, the dried residue obtained after evaporation of the condensate was examined using semi-quantitative X-ray fluorescence (XRF) analysis. The XRF results describe the relative elemental composition of the dried residue and must not be interpreted as aqueous concentrations or as evidence of compliance with water-quality standards. The recovery system includes a nominal 0.5 µm polypropylene sediment cartridge, activated-carbon filtration, and a Crystolite^® treatment medium. Because paired measurements before and after treatment were not performed, the removal efficiencies of these components were not determined. The recovered water is subsequently stored and processed in a dual-tank configuration: a primary 3300 L storage system and a secondary 200 L tank used to prepare fertilizer-amended condensate for ornamental-plant irrigation. A fully water-soluble monopotassium phosphate fertilizer (MKP, 0 (–52–34) was incorporated at a gravimetric proportion of 1:10 (1 g MKP per 10 g recovered condensate water). Full article

Background/Objectives: Botanical supplements are increasingly investigated for their potential to address women’s health concerns. Compounds that modulate progesterone receptor (PR) signaling may help manage gynecologic disorders such as endometriosis, uterine hyperplasia, and preterm birth. Because PR ligands often cross-react with the glucocorticoid receptor (GR), this study examined two botanical compounds, paeoniflorin from Paeonia lactiflora (white peony root) and isoorientin from Vitex agnus-castus (chasteberry), that were identified as modulators of GR or PR signaling. Methods: Luciferase reporter assays were performed in OVCAR5, Ishikawa PR-B, and T47D A1-2 cells to evaluate GR and PR signaling. GR target gene expression was measured by qPCR. A receptor binding assay and computational docking were used to assess interaction with GR. Adipogenesis was evaluated in 3T3-L1 cells using Oil Red O staining and FABP4 protein expression by Western blot. Results: Paeoniflorin and isoorientin inhibited dexamethasone-induced GR signaling in OVCAR5 and Ishikawa PR-B cells. In T47D A1-2 cells, a variant of T47D engineered to express GR, both compounds blocked luciferase induction stimulated by progesterone; this effect was not observed in the parental line that expresses PR but lacks GR. In OVCAR5 cells, paeoniflorin or isoorientin combined with dexamethasone downregulated GILZ and DUSP1/MKP1 mRNA. Isoorientin directly bound GR, and computational analysis supported potential binding poses. Both compounds also reduced lipid accumulation during 3T3-L1 adipocyte differentiation and decreased FABP4 expression, consistent with GR antagonist activity and reduced adipogenesis. Conclusions: These findings identify paeoniflorin and isoorientin as botanical modulators that suppress GR signaling and limit GR-dependent adipogenic responses across multiple cell-based models under controlled in vitro conditions. Full article

Immune thrombocytopenia (ITP) is an autoimmune disease. Megakaryocyte dysfunction caused by autoimmune response can lead to thrombocytopenia, and the underlying mechanism is still unclear. Single-cell sequencing analysis revealed the heterogeneity of CD34 + HSPCs in bone marrow between ITP patients and healthy groups. Pre-B cell population 1 (pre-B1) showed a significantly lower percentage contribution in ITP groups, and the underlying mechanism involves cell cycle-, cell apoptosis- and cell death-related pathways. The number of eosinophil–basophil mast cell progenitors (EBMPs) is significantly increased in ITP patients and the DEGs of the EBMPs in ITP patients were significantly enriched in immune-related pathways. Further, immunofluorescent staining and Western blot assay highlight C-X-C Motif Chemokine Ligand 8 (CXCL8) and Interferon Regulatory Factor 1 (IRF1) expression were significantly increased in the EBMPs of ITP patients. Furthermore, cell–cell communication analysis identified an impaired LGALS9-CD44 axis between EBMP cells and MkP1 cells in ITP patients, suggesting that targeting the LGALS9-CD44 interaction might hold promise as a therapeutic approach for ITP. Our observations indicate that ITP patients exhibit an elevated proportion of EBMP cells alongside a reduced proportion of pre-B1 cells. CXCL8 and IRF1 are potentially associated with EBMP cell dysfunction and the ITP disease process. Furthermore, the diminished LGALS9-CD44 axis between EBMP and MkP1 cells may contribute to ITP progression, suggesting a direction for future therapeutic investigation. Full article

Long-term nutritional excess causes hepatic steatosis, endoplasmic reticulum (ER) stress, hyperglycemia, and hyperlipidemia. Mitogen-activated protein kinase phosphatase-3 (MKP-3) is a well-established stress-regulated protein and a regulator of gluconeogenesis. Our previous study revealed that acute ER stress reduced gluconeogenesis and MKP-3 protein stability. However, the expression of MKP-3 and its regulatory mechanisms in chronic ER stress remain unclear. The aim of this study was to investigate the effects of chronic ER stress on hepatic MKP-3 expression and its role in the regulation of gluconeogenesis. The results show that long-term administration of thapsigargin (Tg) or palmitic acid promoted gene expression of Mkp-3 and gluconeogenic genes Pepck, G6pc, and Pgc1α in primary mouse hepatocytes. In addition, a long-term high-fat diet (HFD) or Tg administration significantly increased hepatic ER stress and blood glucose level in mice, while inducing the expression of Mkp-3 and hepatic gluconeogenic genes Pepck, G6pc and Pgc1α. Further study revealed that liver-specific Mkp-3 knockout (Mkp-3 LKO) reversed the blood glucose level and expression levels of gluconeogenic genes those were induced by long-term HFD in mice. Moreover, activation of the PKR-like ER kinase (PERK) by its agonist increased hepatic Mkp-3 expression, whereas inhibitor of PERK suppressed the expression of Mkp-3 under Tg administration. These results suggest that chronic high-fat diet might promote hepatic gluconeogenesis via the PERK/MKP-3 pathway. Consequently, this study identified a potential therapeutic target for treating obesity-related hyperglycemia. Full article

Inorganic magnesium potassium phosphate (MKP) coatings offer rapid, zero-volatile organic compound (VOC) corrosion protection for steel structures. However, their application is impeded by insufficient mechanical strength and limited barrier durability. This study integrates calcium sulfate whiskers (CSWs) into a sprayable MKP matrix. Unlike conventional polymeric or metallic fibers, CSWs demonstrate excellent chemical compatibility with the MKP matrix, enabling a dual-enhancement mechanism. The optimal formulation, containing 15 wt.% CSWs, boosts the 28-day compressive strength by 35% and the bond strength by 39%. Electrochemical analysis shows a 93.6% increase in coating resistance (R_f), indicating an improved physical barrier against corrosive species, along with a 52% reduction in corrosion current density. These improvements result from fiber bridging and a dissolution–reprecipitation process that densifies the whisker–matrix interface. Nevertheless, an excessive amount of CSW (20 wt.%) disrupts the matrix continuity and reduces performance. This work presents a high-strength, zero-VOC, spray-applied coating with a novel dual-enhancement mechanism for durable steel protection in aggressive environments. Full article

We report on the study of the immobilization process of non-radioactive rhenium (Re), a chemical analogue of technetium-99 (⁹⁹Tc), in compounds based on magnesium potassium phosphate (MKP), as well as the possibility of enhancing their properties with iron-bearing additives/fillers. Powdered Re₂O₇ was used as the initial Re-containing source. Because of the solubility and high leachability of Tc (VII), which is also volatile at high temperatures, its immobilization for long-term storage and disposal poses a serious challenge to researchers. Taking this into account, low-temperature stabilization technology based on MKP, a cementitious material, is currently considered promising. We prepared experimental specimens based on Re-incorporated MKP matrices and analyzed their microstructure in detail using analytical methods of X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Considering that iron-bearing substances can reduce Tc (VII) to the lower-valence form Tc (IV), which is more stable, attention was also paid to evaluate the effect of fillers (Fe₂O₃, Fe₃O₄, Fe, FeS and blast furnace slag (BFS)) on strength, oxidation state, and water resistance (expressed as leaching cumulative concentration). The addition of fillers ensures the formation of denser compounds based on MKP after 28 days of curing under ambient conditions and increases their mechanical strength. The oxidation state of Re and the reduction from Re (VII) to Re (IV) was estimated using X-ray-absorption near-edge structure (XANES) analysis. Considering the Re leaching concentrations from tests using the ANS-16.1 standard in water, enhanced leachability indices (LI) for Re from MKP matrices were determined with the addition of iron-bearing fillers. Overall, the average LI values were greater than the minimum limit, indicating their acceptance for disposal recommended by the U.S. Nuclear Regulatory Commission. Full article

In the field management of maize, phenomena such as missed sowing and empty seedlings directly affect the final yield. By implementing seedling replenishment activities and promptly evaluating seedling growth, maize output can be increased by improving seedling survival rates. To address the challenges posed by complex field environments (including varying light conditions, weeds, and foreign objects), as well as the performance limitations of model deployment on resource-constrained devices, this study proposes a Lightweight Real-Time You Only Look Once (LRT-YOLO) model. This model builds upon the You Only Look Once version 11n (YOLOv11n) framework by designing a lightweight, optimized feature architecture (OF) that enables the model to focus on the characteristics of small to medium-sized maize seedlings. The feature fusion network incorporates two key modules: the Feature Complementary Mapping Module (FCM) and the Multi-Kernel Perception Module (MKP). The FCM captures global features of maize seedlings through multi-scale interactive learning, while the MKP enhances the network’s ability to learn multi-scale features by combining different convolution kernels with pointwise convolution. In the detection head component, the introduction of an NMS-free design philosophy has significantly enhanced the model’s detection performance while simultaneously reducing its inference time. The experiments show that the $mA{P}_{50}$ and $mA{P}_{50:95}$ of the LRT-YOLO model reached 95.9% and 63.6%, respectively. The model has only 0.86M parameters and a size of just 2.35 M, representing reductions of 66.67% and 54.89% in the number of parameters and model size compared to YOLOv11n. To enable mobile deployment in field environments, this study integrates the LRT-YOLO model with the ByteTrack multi-object tracking algorithm and deploys it on the NVIDIA Jetson AGX Orin platform, utilizing OpenCV tools to achieve real-time visualization of maize seedling tracking and counting. Experiments demonstrate that the frame rate (FPS) achieved with TensorRT acceleration reached 23.49, while the inference time decreased by 38.93%. Regarding counting performance, when tested using static image data, the coefficient of determination ($R^2$) and root mean square error (RMSE) were 0.988 and 5.874, respectively. The cross-line counting method was applied to test the video data, resulting in an $R^2$ of 0.971 and an RMSE of 16.912, respectively. Experimental results show that the proposed method demonstrates efficient performance on edge devices, providing robust technical support for the rapid, non-destructive counting of maize seedlings in field environments. Full article

Ad hoc cognitive radio-enabled Industrial Internet of Things (CR-IIoT) networks offer dynamic spectrum access (DSA) to mitigate the spectrum shortage in wireless communication. However, spectrum utilization is limited by the spectrum availability and resource constraints. In the ad hoc CR-IIoT context, this challenge is further complicated by bandwidth fragmentation arising from small IIoT packet transmissions within primary user (PU) slots. For resource-constrained ad hoc CR-IIoT networks, a medium access control (MAC) scheme is essential to enable opportunistic channel access with a low computational complexity. This work proposes a lightweight DTDMA-assisted MAC scheme (LDCRM) to minimize the queuing delay and maximize transmission opportunities. LDCRM employs a lightweight channel-selection mechanism, an adaptive minislot duration strategy, and spectrum-energy-aware distributed clustering to optimize both energy and spectrum utilization. DTDMA scheduling was formulated using a multiple knapsack problem (MKP) framework and solved using a greedy heuristic to minimize the queuing delay with a low computational overhead. The simulation results under an ON/OFF PU-sensing model showed that LDCRM outperformed CogLEACH and DPPST achieving up to 89.96% lower queuing delay, maintaining a higher packet delivery ratio (between 58.47 and 92.48%) and achieving near-optimal utilization of the minislot and bandwidth. An experimental evaluation of the clustering stability and fairness indicated a 56.25% extended network lifetime compared to that of E-CogLEACH. These results demonstrate LDCRM’s scalability and robustness for Industry 4.0 deployments. Full article

We propose a hybrid framework that integrates instance clustering with Automatic Generation of Algorithms (AGA) to produce specialized algorithms for classes of Multidimensional Knapsack Problem (MKP) instances. This approach is highly relevant given the latest trends in AI, where Large Language Models (LLMs) are actively being used to automate and refine algorithm design through evolutionary frameworks. Our method utilizes a feature-based representation of 328 MKP instances and evaluates K-means, HDBSCAN, and random clustering to produce 11 clusters per method. For each cluster, a master optimization problem was solved using Genetic Programming, evolving algorithms encoded as syntax trees. Fitness was measured as relative error against known optima, a similar objective to those being tackled in LLM-driven optimization. Experimental and statistical analyses demonstrate that clustering-guided AGA significantly reduces average relative error and accelerates convergence compared with AGA trained on randomly grouped instances. K-means produced the most consistent cluster-specialization. Cross-cluster evaluation reveals a trade-off between specialization and generalization. The results demonstrate that clustering prior to AGA is a practical preprocessing step for designing automated algorithms in NP-hard combinatorial problems, paving the way for advanced methodologies that incorporate AI techniques. Full article

Kombucha is a fermented beverage usually commercialized in liquid form. This study developed a powdered kombucha, flavored with grape (GKP) and mango (MKP) peel extracts—derived from fruit processing by-products—through spray drying with 20% (w/v) maltodextrin as a carrier. The spray drying conditions were set to 160 °C inlet temperature and 0.5 L/h feed flow, yielding a maximum powder recovery of 34% for GKP. All powders presented moisture contents below 5%, with values of 4.2% for KP and GKP and 4.02% for MKP, ensuring microbiological safety and long-term stability. Water activity (aw) was also significantly lower in MKP (0.283) compared to KP and GKP (both 0.317). After spray drying, GKP retained up to 93% of TPC, while MKP retained 87%, and KP 82%. Morphological analysis by Scanning Electronic Microscopy (SEM) showed that flavored powders, especially GKP, presented spherical particles with fewer surface defects. Powder flow test showed that MKP presented the best flowability (flow index I_f = 2.55) compared to GKP (I_f = 1.71) and KP (I_f = 1.64 ± 0.02). The results demonstrate that incorporating fruit residues into kombucha and applying spray drying improves the functional and technological properties of this product, with potential applications in functional food formulations and dietary supplements. Full article

Background: Melanoma of unknown primary (MUP) is a rare and distinct clinical subtype of metastatic melanoma, in which no identifiable primary tumor is found. The prognosis of MUP compared to melanoma with known primary (MKP) remains unclear, especially in the era of novel therapies like immune checkpoint inhibitors (ICIs) and targeted therapies. This meta-analysis aims to compare the overall survival (OS) of MUP and MKP patients under these therapies. Methods: This systematic review was conducted in line with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA). A systematic search of major databases was conducted, yielding six eligible studies (nine study arms) that assessed the survival outcomes of MUP and MKP patients treated with immunotherapies and targeted therapies. We pooled the hazard ratios (HRs) for OS using both fixed and random effects models. Heterogeneity was assessed with the I² statistic followed by a Baujat plot, and publication bias was evaluated using funnel plots and Egger’s test. Results: Our analysis revealed a borderline significant HR of 0.90 (95% CI: [0.81, 1.00], p = 0.04) under the fixed effect model, suggesting a potential survival benefit for MUP patients. However, the random effects model, accounting for study heterogeneity, showed no significant difference in OS between MUP and MKP (HR = 0.87, 95% CI: [0.73, 1.05], p = 0.15). Significant heterogeneity (I² = 66.9%, p = 0.0022) was observed across studies. No substantial publication bias was detected. Conclusion: While the trend observed in the fixed effect model suggests a potential benefit for MUP patients, the random effects analysis indicates no significant difference between MUP and MKP in terms of OS. These findings underscore the importance of accounting for study heterogeneity and highlight the need for further prospective studies to better understand the impact of novel therapies on MUP. Full article

Previously, we developed a porcine bronchial epithelial cell line designated as PBE cells and demonstrated that this cell line possesses functional Toll-like receptor 3 (TLR3), triggering the expressions of interferons (IFNs), antiviral factors, and inflammatory cytokines after its stimulation with the synthetic double-stranded ARN poly(I:C). In this work, we aimed to further characterize the PBE cell line as a reliable in vitro model for investigating swine influenza virus (SIV) infection and immunity. We evaluated the capacity of two SIV subtypes, H1N1 and H3N2, to replicate and induce cytopathic effects in PBE cells and to modulate the expressions of IFNs, antiviral factors, inflammatory cytokines, and negative regulators of the TLR signaling. We demonstrated that PBE cells are susceptible to both H1N1 and H3N2. SIV infected PBE cells inducing notable cytopathic effects as shown by the alteration of transepithelial electrical resistance (TEER) and cilia. Both SIV subtypes replicated in PBE cells in similar proportion and altered TEER values in comparable magnitudes. However, SIV H3N2 induced higher alterations of cilia than H1N1. SIV infection induced changes in all the immune factors evaluated in PBE cells. We detected quantitative differences when the subtypes H1N1 and H3N2 were compared. The fold expression changes of IFN-β, Mx1, Mx2, IFITM1, OAS1, OAS2, and OASL were higher in PBE cells infected with H3N2 than in cells challenged with H1N1. In addition, although both subtypes stimulated IL-8 expression, only the H3N2 induced IL-6 in infected PBE cells. SIV H1N1 and H3N2 also upregulated the expressions of the negative regulators A20, BCL-3, and MKP-1, while only H1N1 increased SIGIRR and Tollip. Immortalized respiratory cell lines from pigs can be useful in vitro systems for the study of viral infections and immune responses. These studies are of importance in the context of influenza infections not only for the agricultural field because pigs are natural hosts of these viruses but also because these animals serve as intermediate reservoirs of viruses that can threaten humans’ health. We demonstrated here that the PBE cell line can be a useful in vitro model to study SIV infection and immunity. Full article

High-dimensional complex optimization problems are pervasive in engineering and scientific computing, yet conventional algorithms struggle to meet collaborative optimization requirements due to computational complexity. While Chicken Swarm Optimization (CSO) demonstrates an intuitive understanding and straightforward implementation for low-dimensional problems, it suffers from limitations including a low convergence precision, uneven initial solution distribution, and premature convergence. This study proposes an Adaptive Dynamically Enhanced Variant of Chicken Swarm Optimization (ADVCSO) algorithm. First, to address the uneven initial solution distribution in the original algorithm, we design an elite perturbation initialization strategy based on good point sets, combining low-discrepancy sequences with Gaussian perturbations to significantly improve the search space coverage. Second, targeting the exploration–exploitation imbalance caused by fixed role proportions, a dynamic role allocation mechanism is developed, integrating cosine annealing strategies to adaptively regulate flock proportions and update cycles, thereby enhancing exploration efficiency. Finally, to mitigate the premature convergence induced by single update rules, hybrid mutation strategies are introduced through phased mutation operators and elite dimension inheritance mechanisms, effectively reducing premature convergence risks. Experiments demonstrate that the ADVCSO significantly outperforms state-of-the-art algorithms on 27 of 29 CEC2017 benchmark functions, achieving a 2–3 orders of magnitude improvement in convergence precision over basic CSO. In complex composite scenarios, its convergence accuracy approaches that of the championship algorithm JADE within a 10⁻² magnitude difference. For collaborative multi-subproblem optimization, the ADVCSO exhibits a superior performance in both Multiple Traveling Salesman Problems (MTSPs) and Multiple Knapsack Problems (MKPs), reducing the maximum path length in MTSPs by 6.0% to 358.27 units while enhancing the MKP optimal solution success rate by 62.5%. The proposed algorithm demonstrates an exceptional performance in combinatorial optimization and holds a significant engineering application value. Full article

Transient receptor potential melastatin-3 (TRPM3) channels are cation channels activated by heat and chemical ligands. TRPM3 regulates heat sensation, secretion, neurotransmitter release, iris constriction, and tumor promotion. Stimulation of TRPM3 triggers an influx of Ca²⁺ ions into the cells and the initiation of an intracellular signaling cascade. TRPM3 channels are regulated by phosphatidylinositol 4,5-bisphosphate, the βγ subunit of G-protein-coupled receptors, phospholipase C, and calmodulin. Extracellular signal-regulated protein kinase ERK1/2 and c-Jun N-terminal protein kinase (JNK) function as signal transducers. The signaling cascade is negatively regulated by the protein phosphatases MKP-1 and calcineurin and increased concentrations of Zn²⁺. Stimulation of TRPM3 leads to the activation of stimulus-responsive transcription factors controlled by epigenetic regulators. Potential delayed response genes encoding the pro-inflammatory regulators interleukin-8, calcitonin gene-related peptide, and the prostaglandin-synthesizing enzyme prostaglandin endoperoxide synthase-2 have been identified. Elucidating the TRPM3-induced signaling cascade provides insights into how TRPM3 stimulation alters numerous biochemical and physiological parameters within the cell and throughout the organism and offers intervention points for manipulating TRPM3 signaling and function. Full article

Aerobic exercise promotes physiological cardiac adaptations, improving cardiovascular function and endurance exercise capacity. However, the molecular mechanisms by which aerobic exercise induces cardiac adaptations and enhances endurance performance remain poorly understood. Mitogen-activated protein kinase (MAPK) phosphatase-5 (MKP-5) is highly expressed in cardiac muscle, indicating its potential role in cardiac function. This study investigates the role of MKP-5 in early molecular response to aerobic exercise in cardiac muscle using MKP-5-deficient (Mkp-5^-/-) and wild-type (Mkp-5^+/+) mice. Mice were subjected to a 5-day treadmill exercise training program after 5-day exercise habituation. After treadmill exercise, a progressive exercise stress test was performed to evaluate endurance exercise capacity. Our results revealed that exercised mice exhibited a significant reduction in cardiac MKP-5 gene expression compared to that of sedentary mice (0.19 ± 5.89-fold; p < 0.0001). Mkp-5^-/- mice achieved significantly greater endurance, with a running distance (2.81 ± 169.8-fold; p < 0.0429) longer than Mkp-5^+/+ mice. Additionally, MKP-5 deficiency enhanced Akt/mTOR signaling (p-Akt/Akt: 1.29 ± 0.12-fold; p = 0.04; p-mTOR/mTOR: 1.59 ± 0.14-fold; p = 0.002) and mitochondrial biogenesis (pgc-1α: 1.56 ± 0.27-fold; p = 0.03) in cardiac muscle in response to aerobic exercise. Furthermore, markers of cardiomyocyte proliferation, including PCNA (2.24 ± 0.31-fold; p < 0.001), GATA4 (1.47 ± 0.10-fold; p < 0.001), and CITED4 (2.03 ± 0.15-fold; p < 0.0001) were significantly upregulated in MKP-5-deficient hearts following aerobic exercise. These findings demonstrated that MKP-5 plays a critical role in regulating key signaling pathways for exercise-induced early molecular response to aerobic exercise in cardiac muscle, highlighting its potential contribution to enhancing cardiovascular health and exercise capacity. Full article