Search Results (95)

Background: The relationship between sagittal malocclusion, temporomandibular disorders (TMD), and musculoskeletal pain remains uncertain. Methods: Cross-sectional study (April 2020–August 2021) in Małopolska, Poland. Ninety participants (ages 19–35) were classified into Angle Classes I–III (n = 30 each) and examined using RDC/TMD (Axis I/II). A proprietary, nonvalidated, piloted whole-body pain-map questionnaire, presented in anterior and posterior views and subdividing the body into predefined craniofacial, spinal, and limb regions, was used to capture pain presence, Numerical Rating Scale (NRS, 0–10) scores by region, and the total number of painful sites. Group differences were analyzed using χ² and Kruskal–Wallis tests with corresponding effect sizes (measures of association strength). For NRS outcomes, a minimal clinically important difference (MCID)—defined as the smallest difference in NRS considered clinically relevant—was prespecified as approximately 1 point. Results: Occlusal class was not associated with TMD Axis I prevalence. However, sagittal malocclusion—particularly Class III—was linked to a less favorable pain profile. Left temporal pain was more frequent in Class III than in Classes I–II (p = 0.024, Cramér’s V = 0.31, medium effect), and cervical spine pain occurred more often in malocclusion groups than in Class I (p = 0.043, Cramér’s V = 0.26, small effect), indicating statistically significant associations. Cervical pain intensity was higher in Classes II–III than in Class I, with a pooled mean difference—defined as the difference in mean NRS between the combined Classes II–III and Class I—of 1.23 NRS points (95% CI 0.38–2.08), exceeding the ≈1-point MCID and suggesting a clinically important burden. The total number of painful sites was also greater in Class III than in Class I (p = 0.023, η² = 0.09; Δ = 1.40 sites, 95% CI 0.39–2.41), which indicates a statistically significant association with a medium effect size and a higher overall pain burden. Conclusions: Sagittal occlusal class was not associated with TMD diagnosis, but malocclusion—especially Class III—was associated with a more unfavorable craniofacial pain pattern and higher cervical pain burden (p ≤ 0.05), with effects of potential clinical relevance. Full article

Background: Bone mineral density (BMD) assessed by DXA is a well-established predictor of osteoporotic fracture risk. However, data regarding the Polish female population remains limited. Objective: To evaluate the predictive value of BMD measurements for vertebral, hip, and all low-energy fractures in women aged 50 years and older. Methods: A total of 1.311 women from the Małopolska region underwent BMD assessment at the femoral neck, lumbar spine. The average follow-up period was 10.2 years, during which 479 osteoporotic fractures were recorded. Results: DXA measurements at the femoral neck showed the strongest correlation with hip fracture risk. Each one standard deviation decrease in the femoral neck T-score increased the risk of hip fracture by 2.1 times (HR 2.10; 95% CI 1.28–3.46; p = 0.003), after adjusting for age, but is not linear. A 1 SD decrease in the hip T-score was associated with a 28% increase in the risk of all osteoporotic fractures (HR 1.28; CI 1.17–1.40; p < 0.001), 53% increase in vertebral (HR 1.53; CI 1.13–2.08; p = 0.006) and 30% in hip (HR 1.30; CI 0.81–2.09; p = 0.278). The AUC values for hip BMD and hip T-score had the highest predictive value—AUC (area under the curve was 0.732 and 0.720, p < 0.01). Conclusions: BMD at the femoral neck proved to be a stronger predictor of hip fractures than measurements at the spine, radius. The risk increase associated with BMD/T-score reduction was non-linear. These findings confirm results from other benchmark studies. Full article

The study analyzes pro-environmental attitudes and behaviors of residents in the mountainous regions of Małopolska regarding energy saving and transportation. The main objective was to determine the extent to which environmental awareness, vehicle technical condition, and driving style translate into actual energy-efficient behaviors. The research was conducted using a quantitative method among 423 respondents from six mountain districts of Małopolska, based on a proprietary questionnaire and statistical analysis employing non-parametric tests, correlation coefficients, and principal component analysis. The results indicate that respondents most frequently declare simple pro-environmental actions such as waste segregation and energy saving, while less often engaging in activities requiring higher effort or investment, such as eco-driving or limiting car use. Women exhibit higher environmental sensitivity and greater support for ecological regulations, whereas men tend to focus on the technical aspects of vehicle maintenance. The most pro-environmental attitudes and motivations to switch to low-emission vehicles are observed among individuals aged 25–44. The findings confirm that demographic factors significantly differentiate eco–energy-saving orientations, and that environmentally friendly transport behaviors are closely linked to everyday energy-saving practices. Full article

The biogas sector is undergoing development as a result of the growing demand for renewable energy. Methane fermentation allows for the acquisition of energy that is universally usable, while also facilitating the neutralization of problematic waste. Sewage sludge generated as a result of a number of technological processes occurring during wastewater treatment requires appropriate management, and its volume increases every year. In this work, the task was to determine the suitability of sewage sludge for co-digestion with agricultural biomass. The research allowed for the determination of the positive impact of using sewage sludge for fermentation with agricultural biomass. The amount of biogas produced and the methane content were higher compared to the single-component fermentation of agricultural biomass. Mixed sludge had a particularly beneficial effect on fermentation. The largest amount of biogas was obtained from maize silage input and mixed sludge, i.e., 309 Ndm³·k⁻¹ d.m. The methane content in this mixture reached a maximum level of 63%. The least productive was mixture no. 4, consisting exclusively of apple pomace. It produced the smallest amount of biogas (96 Ndm³/kg d.m.) and the process occurred with the greatest delay. The rate of the process was similar for mixtures 3 and 4 for an extended period. In the case of mixture no. 2, there was initially a slightly higher inhibition of the process, but by day 17, it had reached the biogas yield level of mixture 3. The amount of biogas produced for mixtures 2 and 3 was 119 and 133 Ndm³/kg d.m., respectively. From day 22 onwards, the process for all mixtures was coming to an end, with no significant biogas yields observed until the end of the study period. Such a high methane content increases the energy value of biogas, which in practice means a higher yield of electricity and heat from the same amount of feedstock, and thus lower unit costs of energy production. Co-digestion of maize silage, apple pomace, and beet pulp with sewage sludge can be a successful practice in biogas plants. Full article

The current transformation of global energy systems has been the subject of a multi-faceted scientific discourse for years. Researchers focus on technical and technological aspects, seeking new and improved alternatives to current solutions. They also analyse formal and legal frameworks of the changes and evaluate their economic aspects or environmental effects. The public’s attitude towards the changes in light of demanding environmental conditions is investigated the least. In particular, little heed is paid to the opinions of rural populations, especially in Poland. In light of the above, this paper aims to analyse the issue of Poland’s energy transition and the public’s perception of the challenges of environmental protection and the resulting need to improve energy solutions to promote the dissemination of renewable energy sources. The research area was Poland, and detailed research was conducted in five districts (Małopolska region), where the age of the respondents was taken as the differentiating feature. The study was based on a literature review and, at a detailed level, on a diagnostic survey among residents of Wadowicki, Miechowski, Krakowski, Limanowski, and Tarnowski Districts. The 2024 CAPI (Computer Assisted Personal Interviewing) survey involved 300 randomly selected interviewees. The study employed a qualitative and quantitative approach, utilising statistical tools such as Spearman’s rank correlation coefficient analysis, the Kruskal–Wallis rank test, and the nonparametric Mann–Whitney U test. The statistical analysis was supported by IBM’s SPSS v.25. The results show that the majority of the population understand and agree with the need for an energy transition in Poland towards renewable energy. Indications of no opinion or in favour of non-renewable energy in the Polish energy system are distinct. This class of indications is determined by the interviewees’ age and suggests potential for improving public awareness of the matter in the group of mature respondents. Full article

This study aimed to assess how prenatal and early postnatal conditions affect the future reproductive performance of young female pigs (gilts). Female piglets were selected from sows in their 3rd and 4th parity. Vaginal and cervical length (VCL) was measured using a catheter during the first (VCLI) and second (VCLII) oestrus. Gilts were classified based on their litter of origin: fewer than 16 piglets (OS), 16–18 piglets (OM), and more than 18 piglets (OL). The longest VCLI was found in gilts from the OM group, significantly differing from both the OS and OL groups. For VCLII, significant differences were found between OS and the other groups, with OM and OL showing greater values. Litter size at birth did not negatively affect later reproductive potential. A positive correlation was found between VCLII and the number of piglets in the first litter, indicating that the larger the litter of origin, the higher the reproductive productivity of the gilt. The results highlight that development between the first and second oestrus is critical for vaginal and cervical growth, which, in turn, reflects future reproductive performance. These findings suggest that early life conditions, particularly litter of origin, play a key role in determining the productivity of replacement gilts. Full article

Promyelocytic leukemia nuclear bodies (PML-NBs) are dynamic membrane-less organelles (MLOs) located in the nucleus that serve as regulatory hubs for multiple cellular processes. This review examines current understanding of PML-NB structure, assembly mechanisms, and their diverse functional roles. We discuss how PML-NBs interact with chromatin to influence gene expression, regulate transcription factors, and participate in protein quality control. The review highlights their critical functions in tumor suppression, particularly in acute promyelocytic leukemia, and their role in intrinsic antiviral defense against various pathogens. Despite significant advances in the field, key questions remain regarding the mechanistic triggers of PML-NB formation and their common roles across different pathologies. Further elucidation of these aspects may provide valuable insights for developing therapeutic approaches targeting the PML-NB axis in disease treatment. Full article

Background: Metabolic syndrome is a multifaceted condition involving lipid and carbohydrate metabolism disorders and hypertension, increasing the risk of cardiovascular disease and type 2 diabetes. Accurate diagnosis and prevention require an interdisciplinary approach that includes both traditional lab tests and modern, non-invasive health assessments. Methods: This study aimed to evaluate metabolic health in adults from the Małopolska Voivodeship by analyzing the relationships between obesity indicators (BMI, waist circumference) and anthropometric, blood, and salivary biomarkers. Sixty-three participants (36 women, 27 men) aged 40–71 underwent body composition analysis (InBody 770), anthropometric measurements, and biochemical tests of blood and saliva. Assessed parameters included body composition (BMI, BFM, FFM, SMM, PBF, VFA, PA), blood pressure, blood biomarkers (glucose, TG, LDL, HDL, HbA1c, insulin, cortisol), and salivary markers (FRAP, DPPH, urea, amylase activity, protein content, pH, buffering capacity). Results: The results showed a strong correlation between body composition and biochemical markers, but the results of the analyzed salivary biomarkers were inconclusive and, in some cases, contradictory to the findings of other authors. Conclusions: Fat mass, cell integrity, and diastolic pressure were key determinants of waist circumference. Our research confirms the validity of using combined diagnostics, bioimpedance, and blood analysis for a comprehensive assessment of metabolic health and indicates the direction for further research using salivary biomarkers. A holistic approach improves risk assessment and strengthens preventive and therapeutic strategies. However, our pilot study showed that the research requires a larger sample size, especially in order to draw representative conclusions regarding salivary biomarkers and their relationship to metabolic health. Full article

Air pollution poses a significant threat to public health, particularly in urban and industrialized regions. This study investigates the relationship between air quality and the frequency of Emergency Medical Service (EMS) calls in the Małopolska Voivodeship of Poland between 2020 and 2023. Data from over 190 air quality sensors (PM₁₀) were spatially aggregated using both hexagonal grids and administrative boundaries, while EMS call records were filtered to focus on cardiovascular and respiratory incidents. During 2020–2023, a total of 305,142 EMS calls were analyzed, and months with PM₁₀ exceedances showed an average of 1.50 respiratory calls per 1000 residents compared to 1.19 in months without exceedances. Statistical analyses, including Kolmogorov-Smirnov tests and Pearson correlation, were applied to explore temporal and spatial associations. Results indicate a statistically significant increase in EMS calls during periods of elevated air pollution, with the strongest correlation observed for respiratory-related incidents. Comparative analyses between high- and low-pollution municipalities supported the observed relationships. Further analysis indicated that the COVID-19 pandemic may have partially confounded these associations, particularly for respiratory cases, though significant patterns remained even after accounting for pandemic peaks. While limitations related to data gaps and seasonal biases exist, the findings suggest that real-time air pollution data could inform better EMS resource allocation. This research highlights the potential of integrating environmental data into public health strategies to improve emergency response and reduce health risks in polluted regions. Full article

Background: Wound healing and scar management remain significant challenges in dermatology and aesthetic medicine. Recent advances in regenerative medicine have introduced plant-derived exosome-like nanoparticles (PDENs) as potential therapeutic agents due to their bioactive properties. This study examines the clinical application of rose stem cell exosomes (RSCEs) in combination with established treatments for managing different types of scars. Methods: A case series of four patients with different scar etiologies (dog bite, hot oil burn, forehead trauma, and facial laser treatment complications) was treated with RSCEs in combination with microneedling (Dermapen 4.0, 0.2–0.4 mm depth) and/or thulium laser therapy (Lutronic Ultra MD, 8–14 J), or as a standalone topical treatment. All cases underwent sequential treatments over periods ranging from two to four months, with comprehensive photographic documentation of the progression. The efficacy was assessed through clinical photography and objective evaluation using the modified Vancouver Scar Scale (mVSS) and the Patient and Observer Scar Assessment Scale (POSAS), along with assessment of scar appearance, texture, and coloration. Results: All cases demonstrated progressive improvement throughout the treatment course. The dog bite scar showed significant objective improvement, with a 71% reduction in modified Vancouver Scar Scale score (from 7/13 to 2/13) and a 61% improvement in Patient and Observer Scar Assessment Scale scores after four combined treatments. The forehead trauma case exhibited similar outcomes, with a 71% improvement in mVSS score and 55–57% improvement in POSAS scores. The hot oil burn case displayed the most dramatic improvement, with a 78% reduction in mVSS score and over 70% improvement in POSAS scores, resulting in near-complete resolution without visible scarring. The facial laser complication case showed a 75% reduction in mVSS score and ~70% improvement in POSAS scores using only topical exosome application without device-based treatments. Clinical improvements across all cases included reduction in elevation, improved texture, decreased erythema, and better integration with surrounding skin. No adverse effects were reported in any of the cases. Conclusions: This preliminary case series suggests that plant-derived exosome-like nanoparticles, specifically rose stem cell exosomes (RSCEs), may enhance scar treatment outcomes when combined with microneedling and laser therapy, or even as a standalone topical treatment. The documented objective improvements, measured by standardized scar assessment scales, along with clinical enhancements in scar appearance, texture, and coloration across different scar etiologies—dog bite, burn, traumatic injury, and iatrogenic laser damage—suggest that this approach may offer a valuable addition to the current armamentarium of scar management strategies. Notably, the successful treatment of laser-induced complications using only topical exosome application demonstrates the versatility and potential of this therapeutic modality. Full article

Background: Scar formation and impaired wound healing represent significant challenges in dermatology and aesthetic medicine, with limited effective treatment options currently available. Objectives: To evaluate the efficacy and long-term outcomes of Damask rose stem-cell-derived exosome (RSCE) therapy in the management of diverse dermatological conditions, including traumatic wounds, surgical scars, and atrophic acne scars. Methods: We conducted a case series study from June 2023 to November 2024, documenting four cases with different types of skin damage treated with lyophilized RSCE products. Treatment protocols included a variety of delivery methods such as topical application, microneedling, and post-procedure care. Follow-up assessments were performed at intervals ranging from 7 days to 10 months. Results: All patients demonstrated significant improvements in scar appearance, skin elasticity, hydration, and overall tissue quality. In traumatic facial injury, RSCE therapy facilitated reduction in scar contracture and improved functional outcomes. For atrophic acne scars, comparative treatment of facial sides showed enhanced results with RSCE addition. Acute wounds exhibited accelerated healing with reduced inflammation, while chronic wounds demonstrated improved epithelialization and long-term scar quality. Conclusions: This case series provides preliminary evidence suggesting that RSCE therapy may offer significant benefits in wound healing and scar management. The observed improvements in tissue regeneration, inflammatory modulation, and long-term aesthetic outcomes warrant further investigation through controlled clinical trials. Full article

This study tackles the challenge of automatically estimating age from pelvis radiographs. Furthermore, we aim to develop a methodology for applying artificial intelligence to classify or regress medical imagery data. Our dataset comprises 684 pelvis X-ray images of patients, each accompanied by annotations and masks for various regions of interest (e.g., the femur shaft). Radiomic features, e.g., the co-occurrence matrix, were computed to characterize the image content. We assessed statistical analysis, machine learning, and deep learning methods for their effectiveness in this task. Correlation analysis indicated that using certain features in specific regions of interest is promising for accurate age estimation. Machine learning models demonstrated that when using uncorrelated features, the optimal mean absolute error (MAE) for age estimation is 5.20, whereas when employing convolutional networks on the texture feature maps yields the best result of 9.56. Automatically selecting radiomic features for machine learning models achieves a MAE of 7.99, whereas utilizing well-known convolutional architectures on the original image results in a system efficacy of 7.96. The use of artificial intelligence in medical data analysis produces comparable outcomes; however, when dealing with a large number of descriptors, selecting the most optimal ones through statistical analysis enables the identification of the best solution quickly. Full article

Scandinavian erratic boulders in central Poland represent a significant element of the region’s geodiversity, fulfilling important natural, scientific, and cultural functions. As objects of high perceptual value, they integrate into the landscape and provide a wide range of geosystem services. The main objectives of research conducted in two areas of the Małopolska Upland are to determine the concentration of these boulders and identify the geosystem benefits they offer, with particular emphasis on lichen species inhabiting their surfaces. Research has confirmed the currently limited use of geosystem services provided by the 25 erratic boulders studied. However, this may change with growing ecological awareness among local communities, enabling a deeper appreciation of inanimate nature. Erratic boulders have the potential to attract geotourists and thus support economic development (by improving the residents’ quality of life), but this potential requires broader promotion. Although the Central Register of Geosites of Poland is an appropriate platform for their registration, none of the analysed boulders have yet been included. The research findings are also partly directed at local government units to help them recognise the value of erratic boulders for sustainable development, in line with existing legal frameworks and development strategies. The detailed characterisation of 25 boulders may inspire broader initiatives and foster knowledge transfer to support regional development through geotourism. The ability to identify the ecosystem benefits provided by erratic boulders is essential for maintaining ecological balance and sustaining natural processes. However, there is growing evidence of the systematic disappearance of erratic boulders from the landscape, which disrupts geosystem balance and leads to further environmental degradation, negatively affecting human well-being. In light of the lack of effective nature protection measures in the study area, it is proposed that some of these boulders be designated as geological protected features. Such a conservation approach could help maintain ecological balance in the designated area. Full article

Background: Grain protein (GPC) and grain starch (GSC) content in common wheat determines suitability for further end-use processing and is an important quality factor. The level of free asparagine in grains (GFAC) significantly affects suitability for thermal processing. The aim of this genome-wide association study (GWAS) was to identify markers associated (MTA) with the levels of GPC, GSC and GFAC in elite winter wheat breeding lines, and to identify candidate genes. Methods: In total, 344 winter wheat lines were phenotyped and genotyped with DArTseq markers. Results: This GWAS revealed 14 MTAs for GPC, 40 for GSC and 43 for GFAC. The new markers were identified and explained from 6.3% to 12.2% of phenotypic variation. For GPC, the region adjacent to marker 4990459 (QGpc.rut.2D) explained 10.2% of the variation and was stable between two years. The novel gene TraesCS7A03G037500, encoding sucrose synthase involved in starch biosynthesis, was identified in the proximity of QGsc.rut.7A.2. The TraesCS1B03G0736700 gene, coding NAD(P)H dehydrogenase subunit H involved in the mitochondrial electron transport chain, was found in the proximity of QGfac.rut.1B.1. Conclusions: These findings provide valuable insights for elucidating inheritance of GCS, and the identified MTAs provide molecular markers for the reduction of free asparagine and increase of protein content in wheat grains. Full article

Background: Hyaluronic acid (HA)–based bioactive hydrogels have emerged as multifunctional platforms for skin bioregeneration. While traditional mesotherapy using multicomponent substances has been widely practiced for improving skin quality, the time-consuming nature of this approach has led to exploration of alternative delivery methods. Aims: This study evaluated the clinical effectiveness of an HA bioactive hydrogel-based bioregeneration system (containing non-stabilized hyaluronic acid and 14 bioactive ingredients) administered via cannula and its impact on facial skin density as assessed by ultrasound imaging. Methods: We conducted a retrospective review of data from 20 female patients aged 30–42 years who received a single cannula-delivered injection of a bioactive hyaluronic acid hydrogel (TEOSYAL^® Redensity [I]) in the midface region. The formulation combines the structural benefits of hyaluronic acid with the biochemical stimulation provided by amino acids, antioxidants, minerals, and vitamins. Skin density was measured using high-frequency ultrasound at baseline, immediately post-procedure, and at 3–4 weeks follow-up. A control group of seven individuals received no treatment. Results: Ultrasound assessments revealed a statistically significant increase in skin density (92.7%, p < 0.001) within the treated area compared to no significant changes in the control group. This substantial improvement in dermal architecture demonstrates the efficacy of bioactive hydrogels in stimulating fibroblast function and extracellular matrix regeneration. Patient satisfaction was high, with 85% of patients reporting being satisfied or very satisfied. Side effects were minimal, with minor bruising (10%) and transient swelling (15%). Conclusions: Cannula-delivered bioactive hyaluronic acid hydrogel effectively enhances facial skin density with high patient satisfaction and minimal downtime, demonstrating the potential of advanced hydrogel formulations as multifunctional therapeutic platforms that extend beyond traditional applications into aesthetic and regenerative dermatology. Full article