Search Results (83)

In the field management of maize, phenomena such as missed sowing and empty seedlings directly affect the final yield. By implementing seedling replenishment activities and promptly evaluating seedling growth, maize output can be increased by improving seedling survival rates. To address the challenges posed by complex field environments (including varying light conditions, weeds, and foreign objects), as well as the performance limitations of model deployment on resource-constrained devices, this study proposes a Lightweight Real-Time You Only Look Once (LRT-YOLO) model. This model builds upon the You Only Look Once version 11n (YOLOv11n) framework by designing a lightweight, optimized feature architecture (OF) that enables the model to focus on the characteristics of small to medium-sized maize seedlings. The feature fusion network incorporates two key modules: the Feature Complementary Mapping Module (FCM) and the Multi-Kernel Perception Module (MKP). The FCM captures global features of maize seedlings through multi-scale interactive learning, while the MKP enhances the network’s ability to learn multi-scale features by combining different convolution kernels with pointwise convolution. In the detection head component, the introduction of an NMS-free design philosophy has significantly enhanced the model’s detection performance while simultaneously reducing its inference time. The experiments show that the $mA{P}_{50}$ and $mA{P}_{50:95}$ of the LRT-YOLO model reached 95.9% and 63.6%, respectively. The model has only 0.86M parameters and a size of just 2.35 M, representing reductions of 66.67% and 54.89% in the number of parameters and model size compared to YOLOv11n. To enable mobile deployment in field environments, this study integrates the LRT-YOLO model with the ByteTrack multi-object tracking algorithm and deploys it on the NVIDIA Jetson AGX Orin platform, utilizing OpenCV tools to achieve real-time visualization of maize seedling tracking and counting. Experiments demonstrate that the frame rate (FPS) achieved with TensorRT acceleration reached 23.49, while the inference time decreased by 38.93%. Regarding counting performance, when tested using static image data, the coefficient of determination ($R^2$) and root mean square error (RMSE) were 0.988 and 5.874, respectively. The cross-line counting method was applied to test the video data, resulting in an $R^2$ of 0.971 and an RMSE of 16.912, respectively. Experimental results show that the proposed method demonstrates efficient performance on edge devices, providing robust technical support for the rapid, non-destructive counting of maize seedlings in field environments. Full article

Ad hoc cognitive radio-enabled Industrial Internet of Things (CR-IIoT) networks offer dynamic spectrum access (DSA) to mitigate the spectrum shortage in wireless communication. However, spectrum utilization is limited by the spectrum availability and resource constraints. In the ad hoc CR-IIoT context, this challenge is further complicated by bandwidth fragmentation arising from small IIoT packet transmissions within primary user (PU) slots. For resource-constrained ad hoc CR-IIoT networks, a medium access control (MAC) scheme is essential to enable opportunistic channel access with a low computational complexity. This work proposes a lightweight DTDMA-assisted MAC scheme (LDCRM) to minimize the queuing delay and maximize transmission opportunities. LDCRM employs a lightweight channel-selection mechanism, an adaptive minislot duration strategy, and spectrum-energy-aware distributed clustering to optimize both energy and spectrum utilization. DTDMA scheduling was formulated using a multiple knapsack problem (MKP) framework and solved using a greedy heuristic to minimize the queuing delay with a low computational overhead. The simulation results under an ON/OFF PU-sensing model showed that LDCRM outperformed CogLEACH and DPPST achieving up to 89.96% lower queuing delay, maintaining a higher packet delivery ratio (between 58.47 and 92.48%) and achieving near-optimal utilization of the minislot and bandwidth. An experimental evaluation of the clustering stability and fairness indicated a 56.25% extended network lifetime compared to that of E-CogLEACH. These results demonstrate LDCRM’s scalability and robustness for Industry 4.0 deployments. Full article

We propose a hybrid framework that integrates instance clustering with Automatic Generation of Algorithms (AGA) to produce specialized algorithms for classes of Multidimensional Knapsack Problem (MKP) instances. This approach is highly relevant given the latest trends in AI, where Large Language Models (LLMs) are actively being used to automate and refine algorithm design through evolutionary frameworks. Our method utilizes a feature-based representation of 328 MKP instances and evaluates K-means, HDBSCAN, and random clustering to produce 11 clusters per method. For each cluster, a master optimization problem was solved using Genetic Programming, evolving algorithms encoded as syntax trees. Fitness was measured as relative error against known optima, a similar objective to those being tackled in LLM-driven optimization. Experimental and statistical analyses demonstrate that clustering-guided AGA significantly reduces average relative error and accelerates convergence compared with AGA trained on randomly grouped instances. K-means produced the most consistent cluster-specialization. Cross-cluster evaluation reveals a trade-off between specialization and generalization. The results demonstrate that clustering prior to AGA is a practical preprocessing step for designing automated algorithms in NP-hard combinatorial problems, paving the way for advanced methodologies that incorporate AI techniques. Full article

Kombucha is a fermented beverage usually commercialized in liquid form. This study developed a powdered kombucha, flavored with grape (GKP) and mango (MKP) peel extracts—derived from fruit processing by-products—through spray drying with 20% (w/v) maltodextrin as a carrier. The spray drying conditions were set to 160 °C inlet temperature and 0.5 L/h feed flow, yielding a maximum powder recovery of 34% for GKP. All powders presented moisture contents below 5%, with values of 4.2% for KP and GKP and 4.02% for MKP, ensuring microbiological safety and long-term stability. Water activity (aw) was also significantly lower in MKP (0.283) compared to KP and GKP (both 0.317). After spray drying, GKP retained up to 93% of TPC, while MKP retained 87%, and KP 82%. Morphological analysis by Scanning Electronic Microscopy (SEM) showed that flavored powders, especially GKP, presented spherical particles with fewer surface defects. Powder flow test showed that MKP presented the best flowability (flow index I_f = 2.55) compared to GKP (I_f = 1.71) and KP (I_f = 1.64 ± 0.02). The results demonstrate that incorporating fruit residues into kombucha and applying spray drying improves the functional and technological properties of this product, with potential applications in functional food formulations and dietary supplements. Full article

Background: Melanoma of unknown primary (MUP) is a rare and distinct clinical subtype of metastatic melanoma, in which no identifiable primary tumor is found. The prognosis of MUP compared to melanoma with known primary (MKP) remains unclear, especially in the era of novel therapies like immune checkpoint inhibitors (ICIs) and targeted therapies. This meta-analysis aims to compare the overall survival (OS) of MUP and MKP patients under these therapies. Methods: This systematic review was conducted in line with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA). A systematic search of major databases was conducted, yielding six eligible studies (nine study arms) that assessed the survival outcomes of MUP and MKP patients treated with immunotherapies and targeted therapies. We pooled the hazard ratios (HRs) for OS using both fixed and random effects models. Heterogeneity was assessed with the I² statistic followed by a Baujat plot, and publication bias was evaluated using funnel plots and Egger’s test. Results: Our analysis revealed a borderline significant HR of 0.90 (95% CI: [0.81, 1.00], p = 0.04) under the fixed effect model, suggesting a potential survival benefit for MUP patients. However, the random effects model, accounting for study heterogeneity, showed no significant difference in OS between MUP and MKP (HR = 0.87, 95% CI: [0.73, 1.05], p = 0.15). Significant heterogeneity (I² = 66.9%, p = 0.0022) was observed across studies. No substantial publication bias was detected. Conclusion: While the trend observed in the fixed effect model suggests a potential benefit for MUP patients, the random effects analysis indicates no significant difference between MUP and MKP in terms of OS. These findings underscore the importance of accounting for study heterogeneity and highlight the need for further prospective studies to better understand the impact of novel therapies on MUP. Full article

Previously, we developed a porcine bronchial epithelial cell line designated as PBE cells and demonstrated that this cell line possesses functional Toll-like receptor 3 (TLR3), triggering the expressions of interferons (IFNs), antiviral factors, and inflammatory cytokines after its stimulation with the synthetic double-stranded ARN poly(I:C). In this work, we aimed to further characterize the PBE cell line as a reliable in vitro model for investigating swine influenza virus (SIV) infection and immunity. We evaluated the capacity of two SIV subtypes, H1N1 and H3N2, to replicate and induce cytopathic effects in PBE cells and to modulate the expressions of IFNs, antiviral factors, inflammatory cytokines, and negative regulators of the TLR signaling. We demonstrated that PBE cells are susceptible to both H1N1 and H3N2. SIV infected PBE cells inducing notable cytopathic effects as shown by the alteration of transepithelial electrical resistance (TEER) and cilia. Both SIV subtypes replicated in PBE cells in similar proportion and altered TEER values in comparable magnitudes. However, SIV H3N2 induced higher alterations of cilia than H1N1. SIV infection induced changes in all the immune factors evaluated in PBE cells. We detected quantitative differences when the subtypes H1N1 and H3N2 were compared. The fold expression changes of IFN-β, Mx1, Mx2, IFITM1, OAS1, OAS2, and OASL were higher in PBE cells infected with H3N2 than in cells challenged with H1N1. In addition, although both subtypes stimulated IL-8 expression, only the H3N2 induced IL-6 in infected PBE cells. SIV H1N1 and H3N2 also upregulated the expressions of the negative regulators A20, BCL-3, and MKP-1, while only H1N1 increased SIGIRR and Tollip. Immortalized respiratory cell lines from pigs can be useful in vitro systems for the study of viral infections and immune responses. These studies are of importance in the context of influenza infections not only for the agricultural field because pigs are natural hosts of these viruses but also because these animals serve as intermediate reservoirs of viruses that can threaten humans’ health. We demonstrated here that the PBE cell line can be a useful in vitro model to study SIV infection and immunity. Full article

High-dimensional complex optimization problems are pervasive in engineering and scientific computing, yet conventional algorithms struggle to meet collaborative optimization requirements due to computational complexity. While Chicken Swarm Optimization (CSO) demonstrates an intuitive understanding and straightforward implementation for low-dimensional problems, it suffers from limitations including a low convergence precision, uneven initial solution distribution, and premature convergence. This study proposes an Adaptive Dynamically Enhanced Variant of Chicken Swarm Optimization (ADVCSO) algorithm. First, to address the uneven initial solution distribution in the original algorithm, we design an elite perturbation initialization strategy based on good point sets, combining low-discrepancy sequences with Gaussian perturbations to significantly improve the search space coverage. Second, targeting the exploration–exploitation imbalance caused by fixed role proportions, a dynamic role allocation mechanism is developed, integrating cosine annealing strategies to adaptively regulate flock proportions and update cycles, thereby enhancing exploration efficiency. Finally, to mitigate the premature convergence induced by single update rules, hybrid mutation strategies are introduced through phased mutation operators and elite dimension inheritance mechanisms, effectively reducing premature convergence risks. Experiments demonstrate that the ADVCSO significantly outperforms state-of-the-art algorithms on 27 of 29 CEC2017 benchmark functions, achieving a 2–3 orders of magnitude improvement in convergence precision over basic CSO. In complex composite scenarios, its convergence accuracy approaches that of the championship algorithm JADE within a 10⁻² magnitude difference. For collaborative multi-subproblem optimization, the ADVCSO exhibits a superior performance in both Multiple Traveling Salesman Problems (MTSPs) and Multiple Knapsack Problems (MKPs), reducing the maximum path length in MTSPs by 6.0% to 358.27 units while enhancing the MKP optimal solution success rate by 62.5%. The proposed algorithm demonstrates an exceptional performance in combinatorial optimization and holds a significant engineering application value. Full article

Transient receptor potential melastatin-3 (TRPM3) channels are cation channels activated by heat and chemical ligands. TRPM3 regulates heat sensation, secretion, neurotransmitter release, iris constriction, and tumor promotion. Stimulation of TRPM3 triggers an influx of Ca²⁺ ions into the cells and the initiation of an intracellular signaling cascade. TRPM3 channels are regulated by phosphatidylinositol 4,5-bisphosphate, the βγ subunit of G-protein-coupled receptors, phospholipase C, and calmodulin. Extracellular signal-regulated protein kinase ERK1/2 and c-Jun N-terminal protein kinase (JNK) function as signal transducers. The signaling cascade is negatively regulated by the protein phosphatases MKP-1 and calcineurin and increased concentrations of Zn²⁺. Stimulation of TRPM3 leads to the activation of stimulus-responsive transcription factors controlled by epigenetic regulators. Potential delayed response genes encoding the pro-inflammatory regulators interleukin-8, calcitonin gene-related peptide, and the prostaglandin-synthesizing enzyme prostaglandin endoperoxide synthase-2 have been identified. Elucidating the TRPM3-induced signaling cascade provides insights into how TRPM3 stimulation alters numerous biochemical and physiological parameters within the cell and throughout the organism and offers intervention points for manipulating TRPM3 signaling and function. Full article

Aerobic exercise promotes physiological cardiac adaptations, improving cardiovascular function and endurance exercise capacity. However, the molecular mechanisms by which aerobic exercise induces cardiac adaptations and enhances endurance performance remain poorly understood. Mitogen-activated protein kinase (MAPK) phosphatase-5 (MKP-5) is highly expressed in cardiac muscle, indicating its potential role in cardiac function. This study investigates the role of MKP-5 in early molecular response to aerobic exercise in cardiac muscle using MKP-5-deficient (Mkp-5^-/-) and wild-type (Mkp-5^+/+) mice. Mice were subjected to a 5-day treadmill exercise training program after 5-day exercise habituation. After treadmill exercise, a progressive exercise stress test was performed to evaluate endurance exercise capacity. Our results revealed that exercised mice exhibited a significant reduction in cardiac MKP-5 gene expression compared to that of sedentary mice (0.19 ± 5.89-fold; p < 0.0001). Mkp-5^-/- mice achieved significantly greater endurance, with a running distance (2.81 ± 169.8-fold; p < 0.0429) longer than Mkp-5^+/+ mice. Additionally, MKP-5 deficiency enhanced Akt/mTOR signaling (p-Akt/Akt: 1.29 ± 0.12-fold; p = 0.04; p-mTOR/mTOR: 1.59 ± 0.14-fold; p = 0.002) and mitochondrial biogenesis (pgc-1α: 1.56 ± 0.27-fold; p = 0.03) in cardiac muscle in response to aerobic exercise. Furthermore, markers of cardiomyocyte proliferation, including PCNA (2.24 ± 0.31-fold; p < 0.001), GATA4 (1.47 ± 0.10-fold; p < 0.001), and CITED4 (2.03 ± 0.15-fold; p < 0.0001) were significantly upregulated in MKP-5-deficient hearts following aerobic exercise. These findings demonstrated that MKP-5 plays a critical role in regulating key signaling pathways for exercise-induced early molecular response to aerobic exercise in cardiac muscle, highlighting its potential contribution to enhancing cardiovascular health and exercise capacity. Full article

P2MKP Alang-alang Tumbuh Subur is an MSME that produces various types of food made from catfish. Inventory recording in these MSMEs is still carried out using simple Excel, which causes various problems, such as differences in real stock and recording stock, longer recording time, and vulnerable data loss. Therefore, it is necessary to improve the recording system. This research aims to design a management information system that is informative and easily accessible, so that inventory and financial records in these MSMEs can be performed quickly and flexibly. The method used is the agile software development method, which prioritizes communication with related parties with several sprints. This method ensures that the system can be developed quickly, precisely, and in accordance with the wishes of the user. The result of this research is a system that contains product stock management pages, employee data, equipment, and business financial transactions. With CRUD (create, read, update, delete) and report printing features, users can easily change and print reports. In addition, there is an access code feature so that data security is guaranteed. With this system, it is expected that MSMEs can increase the efficiency and accuracy of inventory and financial records and support the smooth operation of the company as a whole. Full article

The development of type 2 diabetes (T2D) is largely dependent on the maintenance of pancreatic islet function and mass. Sexual dimorphism in T2D is evident in many areas, such as pathophysiology, treatment, and prevention. Mitogen-activated protein kinase phosphatase-2 (MKP-2) has a distinct role in the regulation of cell proliferation and the development of metabolic disorders. However, whether there is a causal relationship between MKP-2 and diabetes onset is unclear. The aim of this study was to determine the role of MKP-2 in the regulation of whole-body glucose homeostasis and the impact on pancreatic islet function using streptozotocin-induced pancreatic injury. Here, we show that female mice with whole-body deletion of MKP-2 exhibit hyperglycemia in mouse models treated with multiple low doses of streptozotocin (STZ). In comparison, both male MKP-2 wild-type and knockout mice were hyperglycemic. Consistent with the hyperglycemia, female MKP-2-deficient mice exhibited reduced islet size. Under T2D conditions, MKP-2-deficient mice display enhanced pancreatic JNK and ERK phosphorylation that is associated with the downregulation of genes important for pancreatic islet development and function, Pdx-1 and MafA. Furthermore, we found impaired metabolic flux in adipose tissue that is consistent with hyperglycemia and dysfunctional pancreas. MKP-2 deletion results in reduced Akt activation that is associated with increased adiposity and insulin resistance in female MKP-2 KO mice. These studies demonstrate the critical role of MKP-2 in the development of T2D diabetes in vivo. This suggests that MKP-2 may have a gender-specific role in diabetes development. This discovery raises the possibility that postmenopausal prevention of T2D may benefit from the activation of MKP-2 activity in islet cells. Full article

Background/Objectives: Previously, we reported that high molecular weight hyaluronic acid (HMW-HA) exerts chondroprotective effects by enhancing dual specificity protein phosphatase 10/mitogen-activated protein kinase (MAPK) phosphatase 5 (DUSP10/MKP5) expression and suppressing inflammatory cytokine-induced matrix metalloproteinase-13 (MMP13) expression in a human immortalized chondrocyte line (C28/I2 cells) via inhibition of MAPKs. The aim of this study was to elucidate the molecular mechanisms underlying the enhancement of DUSP10/MKP5 expression by HMW-HA in C28/I2 cells. Methods: C28/I2 cells were treated with HMW-HA, and the activation of intracellular signaling molecules was determined using Western blot analysis. The expression levels of mRNAs and microRNAs (miRNAs) were evaluated through real-time quantitative reverse transcription PCR analysis. Results: HMW-HA treatment induced Akt phosphorylation via interaction with CD44, and pretreatment with specific inhibitors of phosphatidylinositol-3 kinase/protein kinase B (PI3K/Akt) signaling attenuated the HMW-HA-induced expression of DUSP10/MKP5. HMW-HA suppressed the expression of miR-92a, miR-181a, and miR-181d. Loss-of-function and gain-of-function analyses of these miRNAs indicate that miR-92a, miR-181a, and miR-181d negatively regulate DUSP10/MKP5 expression. Moreover, HMW-HA-induced Akt phosphorylation was partially suppressed by miR-181a and miR-181d mimics. Finally, we found that HMW-HA activates RhoA-associated protein kinase (ROK) signaling, which contributes to Akt phosphorylation. Conclusions: These findings suggest that the induction of DUSP10/MKP5 expression by HMW-HA binding to CD44, leading to MMP13 suppression, involves multiple regulatory mechanisms, including PI3K/Akt and RhoA-activated ROK signaling, in addition to miRNA-mediated regulation. Elucidating these detailed molecular mechanisms may reveal novel biological activities that contribute to the therapeutic efficacy of HMW-HA against osteoarthritis. Full article

Several recent studies have evaluated technologies of spent nuclear fuel processing specifically for solidifying transuranic (TRU) waste as a by-product of fission. Of the TRU group, plutonium and the minor actinides will be responsible for the bulk of the radiotoxicity and heat generation of spent nuclear fuel in the long term (300 to 20,000 years). In this study, we investigated magnesium potassium phosphate (MKP)-based compounds as host waste forms for the encapsulation of inactive trivalent Nd and Sm as analogues of the minor trivalent actinides, Am and Cm. Waste forms were fabricated under ambient atmospheric conditions by adding 5 wt.% of substances containing Nd or Sm via the following two routes: powder oxides and aqueous solutions of nitrate salts. Waste form performance was established using strength and aqueous medium leaching tests of MKP-based specimens. The MKP materials were analyzed by X-ray diffraction (XRD), scanning and transmission electron microscopy (SEM and TEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. The waste forms exhibited a compressive strength of ≥30 MPa and were durable in an aqueous environment. The leachability indices for Nd and Sm, as per the ANS 16.1 procedure, were 19.55–19.78 and 19.74–19.89, respectively, which satisfy the acceptable criteria (>6). The results of the present room temperature leaching study suggest that MKPs can be effectively used as a host material to immobilize actinides (Am and Cm) contained in TRU waste. Full article

The extended noncommutative modified KP (exncmKP) hierarchy is firstly constructed, which gives rise to two types of the ncmKP equation with self-consistent sources (ncmKPESCSs). Then, the noncommutative (NC) Miura transformation between the extended noncommutative KP (exncKP) hierarchy and the exncmKP hierarchy is presented, and the quasideterminant solutions of the exncmKP hierarchy are also given. As its byproduct, the quasideterminant solutions of two types of ncmKPESCSs are obtained. The matrix solutions of two types of ncmKPESCSs are finally investigated, and the impact of the source terms on the NC soliton is analyzed. Full article

Manuka essential oil has long been used in traditional medicine, though the effects of the oil on cancer cells have limited studies. The goal of this project was to treat cancer cell lines with manuka essential oil at different concentrations and to ascertain the effects on the cell proliferation of normal fibroblast (CUA-4) and on fibrosarcoma (HT-1080) cells. Cell lines were grown on 24-well plates, and subconfluent cultures were treated with varying concentrations of manuka oil for 24 h. The effect of the oil on proliferation and viability was measured through direct cell counting using trypan blue dye exclusion and through the use of an MTT assay. As the concentration of oil increased, proliferation of all cell lines tested decreased with increasing dosage, concurrently with a decrease in MTT activity. To determine if the decrease in cell numbers observed from manuka oil treatment is the result of apoptosis, PARP cleavage assays were performed, confirming that the treatment caused apoptosis in both normal fibroblasts and fibrosarcoma cells. The stress-activated MAPK protein, JNK, was activated by manuka essential oil treatment, occurring synergistically with a decrease in MKP-1 expression. Full article