Search Results (15)

In this study, we conducted a comparative analysis of the catalytic behavior of Ni-CaO-Al₂O₃ dual functional material (DFM) and a physical mixture of Ni-Al₂O₃ and CaO-Al₂O₃ in the integrated carbon capture methanation (ICCM) process for promoted methane production. H₂-temperature-programmed surface reaction (H₂-TPSR) analysis revealed that in Ni-CaO-Al₂O₃ DFM, CO₂ adsorbed on the CaO surface can spillover to metallic Ni surface, enabling direct hydrogenation without desorption of CO₂. Ni-CaO-Al₂O₃ DFM exhibited a rapid initial methanation rate due to CO₂ spillover. The Ni-CaO-Al₂O₃ DFM, with Ni and CO₂ adsorption sites in close distance, allows efficient utilization of the heat generated by methanation to desorb strongly adsorbed CO₂, leading to enhanced methane production. Consequently, Ni-CaO-Al₂O₃ DFM produced 1.3 mmol/g_Ni of methane at 300 °C, converting 35% of the adsorbed CO₂ to methane. Full article

The quality of supply chains in public health interventions in low- and middle-income countries can determine how effectively a program is able to treat its intended population group and subsequently achieve its health targets. We aimed to disentangle where challenges exist hierarchically and administratively through the application of process mapping to the supply chain of an integrated community case management (iCCM) intervention in the Democratic Republic of the Congo (DRC). We conducted a document review, semi-structured key informant interviews, and focus group discussions with program agents involved in supply chain processes of the child health intervention. Enterprise architecture was used to map the intervention’s supply chain and its participatory actors, and detailed bottlenecks of the chain through the application of a health systems framework. The results of this study will be used to inform a system dynamics model of the supply chain of iCCM in DRC. The greatest bottlenecks leading to stockouts at the community level occurred upstream (from national to province and from zone to health facility). While the use of local procurement processes was partially attempted to strengthen systems, parallel supply chain activities compromised sustainable system integration and development. Initial delays in stock dispensation were due to international procurement at the supplier, inducing a trickle-down effect. Inadequate quantification of supply needs and subsequent insufficient product procuration were the single most important steps that led to stockouts. This study demonstrated that the community health supply chain would be most impacted by improvements made in processes at the highest administrative strata, while exposing its delicate dependence on activities at the lowest levels. Visibility of inventory at all levels and improved data quality and use through a transparent tracking system have the potential to significantly reduce stockouts. Future interventions should take care to not develop parallel processes or exclude local health system agents to avoid disruption and ensure sustainable health outcome gains. Causal loop studies and system dynamics can further identify the systems interactions and relationships and their underlying causal mechanisms in need of intervention. Full article

Cerebral cavernous malformations (CCMs) are relatively common in the central nervous system. They occur in two forms, sporadic and familial (FCCMs). Three genes are recognized to be associated with FCCM, including CCM1, CCM2, and CCM3, the latter also called PDCD10. In this article, we describe a single-nucleotide variant in the PDCD10 gene in a 23-year-old Polish female with CCM. The NM_007217.4 (PDCD10): c.395+1G>A variant destroys the canonical splice donor site following exon 6. This is the first reported genetically characterized case of CCM (FCCM) in Poland. Full article

Cerebral cavernous malformation (CCM) is a neurovascular disease that can lead to seizures and stroke-like symptoms. The familial form is caused by a heterozygous germline mutation in either the CCM1, CCM2, or CCM3 gene. While the importance of a second-hit mechanism in CCM development is well established, it is still unclear whether it immediately triggers CCM development or whether additional external factors are required. We here used RNA sequencing to study differential gene expression in CCM1 knockout induced pluripotent stem cells (CCM1^−/− iPSCs), early mesoderm progenitor cells (eMPCs), and endothelial-like cells (ECs). Notably, CRISPR/Cas9-mediated inactivation of CCM1 led to hardly any gene expression differences in iPSCs and eMPCs. However, after differentiation into ECs, we found the significant deregulation of signaling pathways well known to be involved in CCM pathogenesis. These data suggest that a microenvironment of proangiogenic cytokines and growth factors can trigger the establishment of a characteristic gene expression signature upon CCM1 inactivation. Consequently, CCM1^−/− precursor cells may exist that remain silent until entering the endothelial lineage. Collectively, not only downstream consequences of CCM1 ablation but also supporting factors must be addressed in CCM therapy development. Full article

Irradiation of the tumour site during treatment for cancer with external-beam ionising radiation results in a complex and dynamic series of effects in both the tumour itself and the normal tissue which surrounds it. The development of a spectral model of the effect of each exposure and interaction mode between these tissues would enable label free assessment of the effect of radiotherapeutic treatment in practice. In this study Fourier transform Infrared microspectroscopic imaging was employed to analyse an in-vitro model of radiotherapeutic treatment for prostate cancer, in which a normal cell line (PNT1A) was exposed to low-dose X-ray radiation from the scattered treatment beam, and also to irradiated cell culture medium (ICCM) from a cancer cell line exposed to a treatment relevant dose (2 Gy). Various exposure modes were studied and reference was made to previously acquired data on cellular survival and DNA double strand break damage. Spectral analysis with manifold methods, linear spectral fitting, non-linear classification and non-linear regression approaches were found to accurately segregate spectra on irradiation type and provide a comprehensive set of spectral markers which differentiate on irradiation mode and cell fate. The study demonstrates that high dose irradiation, low-dose scatter irradiation and radiation-induced bystander exposure (RIBE) signalling each produce differential effects on the cell which are observable through spectroscopic analysis. Full article

Children who show better eating practices are less likely to suffer from severe caries than those who eat a diet rich in sugars. In the present study, we aimed to establish the relationship between the severity of dental caries and adherence to the Mediterranean diet. A cross-sectional study was carried out in which 263 children aged 2 to 14 years old were examined intraorally to analyze the presence and severity of caries. Children’s parents/caregivers completed the KIDMED questionnaire to determine their degree of adherence to the Mediterranean diet. The results showed that the prevalence of caries is greater than 80% in children with medium or low adherence to the Mediterranean diet, and remains significant at 67% in the high adherence group (p = 0.010). A statistically significant negative correlation of weak magnitude (r = −0.29; p < 0.001) was found between adherence and the number of carious teeth. Caries severity in the first molars is also influenced by adherence to the diet in a statistically significant way. In conclusion, there is an association between adherence to the Mediterranean diet and the prevalence, extension, and severity of caries in the pediatric population. Full article

The electrical conductivities of aqueous solutions of sodium salts of trans-4-hydroxycinnamic acid (trans-p-coumaric acid), trans-3,4-dihydroxycinnamic acid (trans-caffeic acid), trans-4-hydroxy-3-methoxycinnamic acid, (trans-ferulic acid) and trans-3-phenylacrylic acid (trans-cinnamic acid) with α-cyclodextrin were measured in the temperature range of 288.15 K–318.15 K. For the first time in the literature, using the limiting molar conductivity $\left({\mathsf{\Lambda }}_m^o\right)$ obtained from conductivity measurements, the values of the complexation constants (K_f) of the salts of phenolic acid derivatives with α-cyclodextrin were determined using a modified low concentration chemical model (IcCM). An attempt was also made to analyze the individual thermodynamic functions $\Delta G^o$, $\Delta H^o$ and $\Delta S^o$ describing the complexation process as a function of temperature changes. The obtained results show that the process of formation of inclusion complexes is exothermic and is spontaneous. Full article

A mobile-phone-based diagnostic tool, which most of the population can easily access, could be a game changer in increasing the number of examinations of people with dental caries. This study aimed to apply a deep learning algorithm in diagnosing the stages of smooth surface caries via smartphone images. Materials and methods: A training dataset consisting of 1902 photos of the smooth surface of teeth taken with an iPhone 7 from 695 people was used. Four deep learning models, consisting of Faster Region-Based Convolutional Neural Networks (Faster R-CNNs), You Only Look Once version 3 (YOLOv3), RetinaNet, and Single-Shot Multi-Box Detector (SSD), were tested to detect initial caries lesions and cavities. The reference standard was the diagnosis of a dentist based on image examination according to the International Caries Classification and Management System (ICCMS) classification. Results: For cavitated caries, YOLOv3 and Faster R-CNN showed the highest sensitivity among the four tested models, at 87.4% and 71.4%, respectively. The sensitivity levels of these two models were only 36.9 % and 26% for visually non-cavitated (VNC). The specificity of the four models reached above 86% for cavitated caries and above 71% for VNC. Conclusion: The clinical application of YOLOv3 and Faster R-CNN models for diagnosing dental caries via smartphone images was promising. The current study provides a preliminary insight into the potential translation of AI from the laboratory to clinical practice. Full article

Background: Caries lesion activity is typically assessed by visual–tactile criteria. Regular monitoring is required to detect the transition of lesions and to ensure that the initial assessment was valid. This clinical study aimed to evaluate the agreement of bioluminescence measurements (Calcivis imaging system, Cis) with visual examination to assess caries lesion activity and to monitor occlusal surfaces. Methods: The occlusal surfaces of ninety-one permanent posterior teeth were assessed for the presence or absence of active caries lesions with ICCMS criteria and Cis measurements at three visit times: baseline (t1) and six months (t2) and 12 months (t3) after baseline. Results: At the baseline visit, 70% of the included occlusal sites were assessed visually as active lesions (ICCMS codes 1 and 2). At t3, 64.8% of the sites showed signs of an active lesion. The percentage agreements between the visual and Cis methods were 87.8% (t1), 89.9% (t2) and 88.6% (t3). The corresponding κ-values were 0.71 (95% CI 0.52;0.87), 0.75 (95% CI 0.59;0.89) and 0.77 (95% CI 0.61;0.90), respectively. No significant difference between the visual and bioluminescence systems was found at any visit (p > 0.05). The results based on cluster randomization (generalized estimation equations) showed no significant differences between the visual and Cis findings for all visits (p = 0.108, Wald Χ² with 1 df = 2.587). Conclusion: The bioluminescence system demonstrated substantial agreement for the activity assessment of occlusal lesions compared to the findings obtained by visual assessment over twelve months. Full article

Geographical and economic access barriers to health facilities (HF) have been identified as some of the most important causes of the low coverage of severe acute malnutrition (SAM) treatment. The objective of this study is to assess the effectiveness and coverage of SAM treatment delivered by community health workers (CHWs) in the Guidimakha region in Mauritania, compared to the HF based approach. This study was a nonrandomized controlled trial, including two rural areas. The control group received outpatient treatment for uncomplicated SAM from HF, whilst the intervention group received outpatient treatment for uncomplicated SAM from HF or CHWs. A total of 869 children aged 6–59 months with SAM without medical complications were included in the study. The proportion of cured children was 82.3% in the control group, and 76.4% in the intervention group, we found no significant difference between the groups. Coverage in the intervention zone increased from 53.6% to 71.7%. In contrast, coverage remained at approximately 44% in the control zone from baseline to end-line. This study is the first to demonstrate in Mauritania that the decentralization model of CHWs treating SAM improves acute malnutrition treatment coverage and complies with the international quality standards for community treatment of acute malnutrition. The non-randomized study design may limit the quality of the evidence, but these results could be used by political decision-makers as a first step in revising the protocol for acute malnutrition management. Full article

The present study aimed to assess the effectiveness and impact on treatment coverage of integrating severe acute malnutrition (SAM) treatment at the health hut level by community health workers (CHWs). This study was a non-randomized controlled trial, including two rural communes in the health district of Mayahi: Maïreyreye (control) and Guidan Amoumoune (intervention). The control group received outpatient treatment for uncomplicated SAM from health facilities (HFs), while the intervention group received outpatient treatment for uncomplicated SAM from HFs or CHWs. A total of 2789 children aged 6–59 months with SAM without medical complications were included in the study. The proportion of cured children was 72.1% in the control group, and 77.2% in the intervention group. Treatment coverage decreased by 8.3% in the control area, while the group of CHWs was able to mitigate that drop and even increase coverage by 3%. This decentralized treatment model of acute malnutrition with CHWs allowed an increase in treatment coverage while maintaining a good quality of care. It also allowed the early inclusion of children in less severe conditions. These results may enhance the Niger Ministry of Health to review the management of SAM protocol and allow CHWs to treat acute malnutrition. Full article

Chickpea (Cicer arietinum L.) is a cheap source of protein and rich in minerals for people living in developing countries. In order to assess the existing molecular genetic diversity and determine population structures in selected Ethiopian chickpea germplasm accessions (118), a set of 46 simple sequence repeat (SSR) markers equally distributed on the chickpea genome were genotyped. A total of 572 alleles were detected from 46 SSR markers, and the number of alleles per locus varied from 2 (ICCM0289) to 28 (TA22). The average number of alleles per locus, polymorphism information content, and expected heterozygosity were 12, 0.684, and 0.699, respectively. Phylogenetic analysis grouped the 118 chickpea genotypes from diverse sources into three evolutionary and/or biological groups (improved desi, improved kabuli, and landraces). The population structure analysis revealed six sub-populations from 118 chickpea genotypes studied. AMOVA revealed that 57%, 29%, and 14% of the total genetic variations were observed among individuals, within populations, and among populations. The insights into the genetic diversity at molecular levels in the Ethiopian germplasm lines can be used for designing conservation strategies as well as the diverse germplasm lines identified in this study can be used for trait dissection and trait improvement. Full article

(1) Background: The Ministry of Health in Mali included the treatment of severe acute malnutrition (SAM) into the package of activities of the integrated community case management (iCCM). This paper evaluates the most effective model of supervision for treating SAM using community health workers (CHWs). Methods (2): This study was a prospective non-randomized community intervention trial with two intervention groups and one control group with different levels of supervision. It was conducted in three districts in rural areas of the Kayes Region. In the high supervision group, CHWs received supportive supervision for the iCCM package and nutrition-specific supervision. In the light supervision group, CHWs received supportive supervision based on the iCCM package. The control group had no specific supervision. (3) Results: A total of 6112 children aged 6–59 months with SAM without medical complications were included in the study. The proportion of cured children was 81.4% in those treated by CHWs in the high supervision group, 86.2% in the light supervision group, and 66.9% in the control group. Children treated by the CHWs who received some supervision had better outcomes than those treated by unsupervised CHWs (p < 0.001). There was no difference between areas with light and high supervision, although those with high supervision performed better in most of the tasks analyzed. (4) Conclusions: Public policies in low-income countries should be adapted, and their model of supervision of CHWs for SAM treatment in the community should be evaluated. Full article

In this paper, the K-user interference channel with secrecy constraints is considered with delayed channel state information at transmitters (CSIT). We propose a novel secure retrospective interference alignment scheme in which the transmitters carefully mix information symbols with artificial noises to ensure confidentiality. Achieving positive secure degrees of freedom (SDoF) is challenging due to the delayed nature of CSIT, and the distributed nature of the transmitters. Our scheme works over two phases: Phase one, in which each transmitter sends information symbols mixed with artificial noises, and repeats such transmission over multiple rounds. In the next phase, each transmitter uses the delayed CSIT of the previous phase and sends a function of the net interference and artificial noises (generated in previous phase), which is simultaneously useful for all receivers. These phases are designed to ensure the decodability of the desired messages while satisfying the secrecy constraints. We present our achievable scheme for three models, namely: (1) K-user interference channel with confidential messages (IC-CM), and we show that $\frac{1}{2}(\sqrt{K}-6)$ SDoF is achievable; (2) K-user interference channel with an external eavesdropper (IC-EE); and (3) K-user IC with confidential messages and an external eavesdropper (IC-CM-EE). We show that for the K-user IC-EE, $\frac{1}{2}(\sqrt{K}-3)$ SDoF is achievable, and for the K-user IC-CM-EE, $\frac{1}{2}(\sqrt{K}-6)$ is achievable. To the best of our knowledge, this is the first result on the K-user interference channel with secrecy constrained models and delayed CSIT that achieves an SDoF which scales with $\sqrt{K}$ , square-root of number of users. Full article

We investigate the secure degrees of freedom (s.d.o.f.) of three new channel models: broadcast channel with combating helpers, interference channel with selfish users, and multiple access wiretap channel with deviating users. The goal of introducing these channel models is to investigate various malicious interactions that arise in networks, including active adversaries. That is in contrast with the common assumption in the literature that the users follow a certain protocol altruistically and transmit both message-carrying and cooperative jamming signals in an optimum manner. In the first model, over a classical broadcast channel with confidential messages (BCCM), there are two helpers, each associated with one of the receivers. In the second model, over a classical interference channel with confidential messages (ICCM), there is a helper and users are selfish. By casting each problem as an extensive-form game and applying recursive real interference alignment, we show that, for the first model, the combating intentions of the helpers are neutralized and the full s.d.o.f. is retained; for the second model, selfishness precludes secure communication and no s.d.o.f. is achieved. In the third model, we consider the multiple access wiretap channel (MAC-WTC), where multiple legitimate users wish to have secure communication with a legitimate receiver in the presence of an eavesdropper. We consider the case when a subset of users deviate from the optimum protocol that attains the exact s.d.o.f. of this channel. We consider two kinds of deviation: when some of the users stop transmitting cooperative jamming signals, and when a user starts sending intentional jamming signals. For the first scenario, we investigate possible responses of the remaining users to counteract such deviation. For the second scenario, we use an extensive-form game formulation for the interactions of the deviating and well-behaving users. We prove that a deviating user can drive the s.d.o.f. to zero; however, the remaining users can exploit its intentional jamming signals as cooperative jamming signals against the eavesdropper and achieve an optimum s.d.o.f. Full article