Search Results (1,664)

Astaxanthin (AST) is a carotenoid with powerful antioxidant and anti-inflammatory properties and increasing interest in dermatological and nutraceutical applications. In this study, AST-rich extracts were obtained from by-products of the blue crab Callinectes sapidus and chemically characterized using HPLC-DAD analysis. The antioxidant activity of the extracts was assessed through complementary spectrophotometric assays (DPPH and FRAP). Comparable AST contents were detected in the two extracts, with values of 1.269 ± 0.006 and 1.219 ± 0.015 mg/100 g dry weight for EtOH and IPrOH, respectively. However, the EtOH extract exhibited higher antioxidant activity, reaching 0.10 ± 0.01 mg Trolox equivalents (TE)/g in the DPPH assay and 0.27 ± 0.02 mg TE/g in the FRAP assay, compared with 0.08 ± 0.01 and 0.11 ± 0.03 mg TE/g for the IPrOH extract. The biological activity of AST extracts was further evaluated against the opportunistic pathogen Staphylococcus aureus and beneficial lactic acid bacteria. AST exhibited moderate antibacterial activity against S. aureus, with an MIC value of 50 μg/mL and inhibition zones up to 14 mm at 200 μg/disc, while promoting the proliferation of Lactobacillus plantarum, Lactobacillus casei, and Lactobacillus reuteri. These findings highlight the prospective valorization of blue crab by-products as a sustainable supply of antioxidant and microbiota-modulating compounds with possible applications in skin health and cosmetic formulations. Full article

The Cytisus striatus species is a biologically important plant recognised for its high flavonoid content. However, the chemical composition of this species has yet to be fully described. The purpose of this study was to identify flavonoids and related secondary metabolites using advanced analytical techniques, including gas chromatography, liquid chromatography, and NIR and Raman spectroscopy. Ethanolic extracts of flowers, fruits, and twigs/leaves contain highly beneficial flavonoids, with chrysin being the main compound detected in all plant parts. It was quantified by HPLC-DAD at levels ranging from 0.90 to 2.27 mg/g extract in samples collected from three different locations. NIR and FT-RAMAN analysis provided complementary information on the overall chemical fingerprint of the plant material. PCA of the spectroscopic data revealed minor site-related differences in global spectral profiles, with PC1 explaining 85% of the variability in flowers and fruits and 72% in twigs/leaves for FT-NIR, while FT-RAMAN analysis of fruit extracts showed a PC1 accounting for 97% of the variance. This study provides a basis for future research on the biological properties of Cytisus striatus and for further assessment of its potential relevance in pharmaceutical and/or food-related applications. Full article

Background: This study investigated the effects of increasing levels of salinity on leaf phytochemical composition and the antioxidant, antidiabetic, and anti-obesity activities. Method: Three quinoa accessions grown under escalating NaCl treatments had their leaves exposed to various chemical analyses. Polyphenols, tannins, and flavonoids were among the phenolic substances whose concentrations were measured. The phenolic chemicals in the water extract were identified using HPLC-DAD-ESI-MS/MS. In vitro and in silico methods were used to measure anti-radical (DPPH), anti-alpha glucosidase, anti-alpha-amylase, and anti-lipase activities. Results: The results showed that water and ethanol, due to their polarity, were the most effective solvents for extracting phenolic compounds. Additionally, salt application led to a dose-dependent increase in total phenols (TPC), flavonoids (TFC), and tannins (CT) across all accessions. The accession DE-1 exhibited the highest contents with average values of 1453.03–4398.36 mg EGA/100 g DW, 322.7–1090.7 mg CAE/100 g DW, and 77.9–335.96 mg CAE/100 g DW of TPC, TFC, and Tannins, respectively. HPLC-ESI-DAD-MS/MS profiling of phenolic compounds led to identifying 18 constituents, including five major compounds (p-coumaric acid, caffeic acid, vanillic acid, p-coumaroyl hexose, and HHDP-galloyl glucose). Except for p-coumaroyl hexose and HHDP-galloyl glucose, which were extensively biosynthesized/accumulated in the salt-tolerant accession DE-1, the remaining phenolic compounds showed irregular evolution depending on accession and salt concentration. Moreover, ethanol and water extracts were evaluated for their anti-radical and enzyme-inhibitory activities. Conclusion: Salt-stressed DE-1 water extract showed strong antioxidant and enzyme inhibitory activities, indicating potential antidiabetic and anti-obesity effects. These activities were confirmed by in silico analysis. Full article

Accurate profiling of amino acids and selenoamino acids is crucial for evaluating the nutritional quality of selenium-enriched crops. To provide a reliable and accessible tool for routine food monitoring, this study employed pre-column derivatization high performance liquid chromatography (HPLC) method for the simultaneous determination and compositional analysis of 17 standard amino acids, selenocystine (SeCys₂), and selenomethionine (SeMet) in various organs of selenium-enriched radish. Chromatographic separation was performed using a C18 column and a mobile phase of sodium acetate buffer (pH 5.25) and acetonitrile under gradient elution, with diode array detection (DAD) at 360 nm. Method validation demonstrated excellent linearity (R²) ≥ 0.995 for all 19 amino acids within their tested ranges. The limits of detection (LODs) and limits of quantitation (LOQs) were 0.06 to 0.21 mg/L and 0.19 to 0.68 mg/L, respectively. The spike recoveries ranged from 88.2% to 101.7%, while the intra-day and inter-day relative standard deviations (RSDs) were ≤3.09% and ≤4.25%, respectively. The levels of total, essential, selenoamino and taste-active amino acids in the leaves exceeded those in the taproot, with the highest total content of 2398.41 mg/kg found in leaves at the primary growth stage of the taproot. The total content of selenoamino acids ranged from 2.65 to 6.78 mg/kg. This method enables the simultaneous quantification of various amino acids, including selenoamino acids, in different organs of selenium-enriched radish throughout its entire growth period, providing a theoretical basis for the development of selenium-fortified products. Full article

The valorization of agro-industrial by-products is of increasing importance within circular economy strategies. Aronia melanocarpa pomace, a by-product of juice production, represents a valuable source of polyphenols with potential applications in food, nutraceutical, and cosmetic formulations. This study aimed to evaluate the effect of different preservation methods on the polyphenolic composition of Aronia pomace and to optimize accelerated solvent extraction (ASE). Pomace samples were subjected to drying, freeze-drying, freezing (−18 °C), and deep freezing (−80 °C). UAE was applied as a rapid screening method for polyphenol extraction, while ASE was used as an advanced technique for efficient recovery of target compounds. ASE parameters, including temperature (40–120 °C), methanol concentration (40–100%), and number of extraction cycles (1–3), were optimized using response surface methodology (RSM) based on a Box–Behnken design. Qualitative and quantitative analyses were performed using UHPLC–MS and HPLC–DAD. The developed models were statistically significant (p < 0.01) with high coefficients of determination (R² = 0.88–0.97). Temperature had a positive effect on phenolic acid extraction but negatively affected anthocyanins due to thermal degradation. Optimal extraction conditions differed between compound groups: phenolic acids were maximized at 120 °C and 75% methanol (two cycles), while anthocyanins required milder conditions (82 °C, 92% methanol, three cycles). Moreover, our study showed that drying significantly reduced the content of several compounds, particularly anthocyanins, whereas low-temperature methods had minimal impact. The results highlight the importance of tailored extraction strategies and support the sustainable utilization of Aronia pomace as a source of bioactive compounds. Full article

This study presents a comparative evaluation of solar cabinet drying and traditional open-air sun drying with respect to their influence on the retention of water-soluble vitamins and flavonoids in Plantago major L. leaves, aiming to identify an effective and sustainable drying strategy for functional food applications. Freshly harvested leaves were subjected to both drying methods under comparable environmental conditions. To account for possible structural heterogeneity, external and internal leaf tissues were analyzed separately. Qualitative and quantitative determination of bioactive compounds was performed using high-performance liquid chromatography with diode-array detection (HPLC-DAD). Flavonoids were analyzed at detection wavelengths of 254 and 276 nm, while water-soluble vitamins (C, B₂, B₃, B₆, and B₉) were determined at 250 nm. Quantification was carried out using external calibration, and results were expressed as concentrations (mg/g dry matter). The results demonstrate that solar cabinet drying provides superior preservation of oxidation- and light-sensitive bioactive compounds compared to open-air sun drying. In particular, vitamin C content in solar cabinet-dried samples reached 91.62 mg/g, which was more than three times higher than that observed after open-air drying (26.90 mg/g). Solar cabinet drying also enhanced the retention of key antioxidant flavonoids, notably dihydroquercetin (14.23 mg/g vs. 11.21 mg/g) and luteolin (0.38 mg/g vs. 0.26 mg/g). Although slightly higher concentrations of certain compounds, such as rutin and vitamins B₆ and B₉, were detected in open-air-dried samples, the overall nutraceutical profile favored solar cabinet drying. In conclusion, the controlled microclimate of the solar cabinet dryer significantly improves the stability and retention of critical water-soluble vitamins and antioxidant flavonoids in Plantago major L. leaves. These findings confirm that solar cabinet drying is a nutritionally advantageous, energy-efficient, and sustainable approach for producing high-quality plant-based ingredients suitable for functional food and nutraceutical applications. Full article

In the sustainability framework, valorization of organic by-products as reservoirs of phytochemicals useful for human health represents a hot topic. Therefore, this study evaluated Norway spruce bark and twigs (NSB, NST), chestnut tree wood (CTW), and pomegranate fruit waste/pomace (PFW) as sources of bioactive compounds by employing green technologies. Microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), applied individually or sequentially, were optimized by modulating solvent composition, temperature, time, microwave power, and ultrasound amplitude. Hydroalcoholic extraction (50% ethanol) combined with MAE yielded the highest phenolic recovery and antioxidant activity across all matrices. PFW exhibited the highest antioxidant activity assessed through FRAP, ORAC, and DPPH assays. Phytochemical profiling by HPLC-DAD identified stilbenes in spruce extracts, ellagic acid in chestnut wood, and ellagic acid and punicalagins in pomegranate waste as major bioactive constituents. Additionally, NSB and PFW exhibited α-amylase inhibitory activity. Antimicrobial testing demonstrated dose-dependent activity against Gram-positive (Staphylococcus epidermidis and Bacillus subtilis) and Gram-negative (Pseudomonas stutzeri) strains, with PFW exhibiting the strongest inhibition and NSB displaying broad-spectrum effects. Total phenolic content changed moderately after 21 days of storage. These results demonstrate that sustainable extraction enables efficient recovery of bioactive compounds from plant by-products, supporting their further functional, dietary, and medicinal applications. Full article

Invasive plant species are increasingly recognized not only as ecological threats but also as potential sources of bioactive compounds with agricultural applications. However, the combined allelopathic and insecticidal potential of Ailanthus altissima’s different plant parts remains insufficiently explored. This study evaluated the bioactivity of different plant part (leaf, bark, and branch) extracts of A. altissima. Secondary metabolites were characterized by HPLC–DAD–MS, while ethanol extracts (0.5–5%) were tested on wheat (Triticum aestivum) seed germination, seedling growth, oxidative status, and on the survival and repellency of the rice weevil (Sitophilus oryzae). Biological responses were strongly plant part and concentration-dependent. Leaf extracts contained the highest phenolic levels, dominated by caffeoylquinic acids and quercetin derivatives, whereas bark and branch extracts showed lower but compositionally distinct profiles. Despite this, bark and branch extracts produced the strongest biological effects, inhibiting germination energy and root growth at higher concentrations, while leaf extracts stimulated seedling performance, including increased vigor index, while in insect bioassays, bark and branch extracts caused higher mortality and stronger suppression of rice weevil populations. This study provides new evidence that biomass extracts of the invasive species A. altissima represent a promising source of biologically active compounds with both allelopathic and insecticidal properties, highlighting its potential valorization as a plant-based biopesticide for sustainable pest management. Full article

Green husks, which are the fleshy pericarp of Juglans regia L. fruit, are an abundant yet under-utilized source of bioactive compounds. They are useful for plant defense and have potential for valorization to multiple commercial products. This study characterized total phenolic content (TPC) and individual phenolics in green husks of four Hungarian-bred cultivars (Milotai 10, Milotai intenzív, Milotai kései, Esterhazy kései) and one U.S. cultivar (Chandler). Phenolic compounds were extracted with aqueous organic solvents, quantified by HPLC-DAD and qualitatively identified by HPLC-MS. Linear mixed-effects models were used to assess the effects of cultivar, year, sampling time, and cumulative growing degree days (GDDs) on TPC and compound profiles. Mean TPC ranged from 34.9 to 57.2 mg GAE g⁻¹ DW, with significantly higher values in the warmest year, 2024, and in cultivar Esterhazy kései compared with Chandler. Across cultivars and years, phenolic levels were generally elevated at early lignification (S1, BBCH 73–75) and at full maturity (S5–S6, BBCH 87–88), with depressed concentrations during mid-fruit development (S2–S4, BBCH 77–86). Several hydroxycinnamic acids, flavonoids, and naphthoquinones showed cultivar-specific and year-dependent patterns. Thermal conditions (cumulative GDDs) explained a substantial proportion of residual variation in TPC. These results highlight the combined roles of genotype, seasonal climate, and developmental stage dependencies in biosynthetic processes of phenolics in walnut green husks despite the diversity in factor effects. Full article

Plum fruits are a valuable raw material to produce fermented beverages and a source of phenolic compounds with antioxidant properties. However, information on changes in phenolic composition during plum wine production is still limited. In this study, the evolution of phenolic compounds and antioxidant capacity during maceration and fermentation of wine from the ‘Stanley’ cultivar was investigated. Total phenolics, flavonoids, anthocyanins, and antioxidant capacity were determined spectrophotometrically, while individual compounds were identified by HPLC–DAD analysis. Eleven phenolic compounds were detected, including anthocyanins, hydroxycinnamic acids, and flavonols. Neochlorogenic acid, cyanidin-3-rutinoside, and rutin were the predominant compounds in fruits and wines. Phenolic content in plum skin was more than twofold higher than in whole fruit (445.20 vs. 198.32 mg GAE/100 g FW), with markedly higher anthocyanins (180.08 vs. 36.73 mg CGE/100 g FW), while juice showed much lower levels (89.32 mg GAE/L and 1.08 mg CGE/L). Maceration increased phenolic content and antioxidant activity, whereas fermentation led to a gradual decrease in most compounds, likely due to polymerization and degradation reactions. The wine produced contained 10.80 ± 0.15% (v/v) ethanol. Principal component analysis differentiated samples according to phenolic profile and fermentation stage. Full article

Background/Objectives: Sexual dysfunction is a prevalent and multifactorial condition affecting a large proportion of the global population, with limited therapeutic options beyond pharmacological approaches primarily targeting erectile dysfunction. This has increased interest in botanical supplements for sexual health, although mechanistic evidence and clear links between phytochemical composition and biological activity remain scarce. The present study provides an integrative evaluation of a commercial Turnera diffusa extract (Liboost^®) formulated to support sexual health by combining detailed phytochemical characterization with targeted in vitro mechanistic assays. Methods: The extract was characterized by HPLC-DAD-HRMS, enabling the identification and semi-quantification of its major constituents. A total of 49 compounds were detected, predominantly flavonoids, including luteolin- and apigenin-derived glycosides, flavonols, methoxyflavones, flavanones, and coumaroyl derivatives, with a total quantified flavonoid content of 15.9 mg·g⁻¹. Biological activity was evaluated in human cell models without cytotoxic effects at the tested concentrations. Results: Liboost^® significantly reduced PDE5 expression, inhibited aromatase activity, and moderately increased nitric oxide production. These complementary effects suggest a multi-target modulation of pathways involved in sexual function, integrating vascular, endocrine, and nitrergic mechanisms. Conclusions: Although limited to in vitro models, the findings provide mechanistic support for the biological activity of T. diffusa extracts and highlight the importance of linking phytochemical composition with functional evidence when evaluating botanical supplements. Full article

Gamma-aminobutyric acid (GABA), a non-protein amino acid, functions as the chief inhibitory neurotransmitter in mammals and is associated with several health benefits, including hypotensive, diuretic, tranquilizing, and antidiabetic effects. Although cocoa has been identified as a potential source of GABA, information regarding its concentration in cocoa-derived materials remains limited. This study evaluated the GABA content of dried germinated cocoa beans and dried cocoa husks (shells) collected from different geographical regions in Thailand. GABA was extracted using solid–liquid extraction with deionized water, 70% ethanol (v/v), and 90% ethanol (v/v), and quantified by high-performance liquid chromatography coupled with diode array detection (HPLC/DAD). The results revealed that both germinated cocoa beans and cocoa husks contain considerable amounts of GABA. The highest GABA content in cocoa beans was detected in samples from Tak province (242 ± 18 mg/100 g), while cocoa husks from Nan province exhibited the highest GABA content (361 ± 11 mg/100 g), both obtained using 70% ethanol extraction. Among the solvents tested, 70% ethanol demonstrated superior extraction efficiency compared with deionized water and 90% ethanol. In conclusion, germinated cocoa beans and cocoa husks represent promising natural sources of GABA, and extraction with 70% ethanol provides an effective approach for maximizing GABA recovery for potential functional foods and bioactive product development. Full article

The increasing occurrence of antibiotic residues in poultry meat represents a serious food safety concern associated with antimicrobial resistance and potential risks to human health. This study investigated the effects of electron beam irradiation on antibiotic residues and nutritional quality parameters of poultry meat. All experiments and data collection were carried out in 2025. Fresh poultry samples were irradiated using an ILU-10 pulsed linear electron accelerator at doses of 2, 4, 6, 8, and 10 kGy. Antibiotic residues were determined by HPLC-DAD, amino acid composition was analyzed using HPLC, and fatty acid profiles were evaluated by gas chromatography. Electron beam irradiation produced significant dose-dependent changes in the chemical composition of poultry meat. Total amino acid content decreased progressively with increasing irradiation dose, with reductions of up to 60–73% at 10 kGy depending on tissue type. Branched-chain and essential amino acids showed similar trends. Fatty acid analysis revealed a shift toward higher proportions of saturated fatty acids and a decline in monounsaturated and polyunsaturated fatty acids. The PUFA/SFA ratio decreased from 0.48 in control samples to 0.25 at 10 kGy. Tetracycline residues were not detected in any samples, whereas chloramphenicol residues were present in control meat but were progressively reduced after irradiation and became undetectable at doses ≥ 8 kGy. These results demonstrate that electron beam irradiation can effectively reduce antibiotic residues in poultry meat; however, higher irradiation doses may significantly alter amino acid and lipid composition. Therefore, optimization of irradiation parameters is necessary to balance improvements in food safety with the preservation of nutritional quality for the production of safe and sustainable food products. Optimization of irradiation parameters is therefore necessary to balance food safety benefits with preservation of nutritional quality. Furthermore, this research contributes to the achievement of Sustainable Development Goal (SDG) 2, while the obtained results also support SDG 3 by promoting safer food systems and protecting public health. Full article

Extra virgin olive oil (EVOO) is a key component of the Mediterranean diet, valued for its bioactive constituents and associated health benefits. This study evaluated the influence of four agronomic and processing factors—harvest month, destoning, fruit washing, and bottling delay—on the chemical composition of Kolovi EVOOs from the PGI Lesvos region. A total of 34 oils were produced under standardized conditions and analyzed for phenolic compounds, tocopherols, pigments, and squalene using UPLC-QTOF-MS and HPLC-DAD. The oils were characterized by consistently high nutritional quality, with most samples fulfilling EFSA health claim thresholds for hydroxytyrosol, tyrosol and its derivatives, and α-tocopherol. Harvest month was the most influential parameter: early harvested oils (October) contained significantly higher levels of phenolics, α-tocopherol, and lutein, whereas later harvests (November) were richer in squalene. Destoning produced modest changes, with slightly higher phenolics in non-destoned oils and reduced lipophilic antioxidants in destoned samples. Fruit washing selectively decreased hydrophilic phenolics, while lipophilic compounds were largely unaffected. Bottling delays of up to 48 h under protective conditions had negligible effects on composition, aside from minor increases in specific phenolic derivatives. These findings suggest that early harvesting and careful consideration of destoning are the most effective strategies for supporting the antioxidant profile of Kolovi EVOOs, while other practices can be adjusted with limited impact on quality. Full article

Dietary phytogenic additives and microalgae are increasingly investigated as natural alternatives to antibiotic growth promoters in rabbit production due to their potential effects on gut health and nutrient utilisation. This study evaluated the nutraceutical potential of Chlorella vulgaris and Laurus nobilis as plant-based additives for growing New Zealand White rabbits. A 45-day feeding trial was conducted using control and experimental diets enriched with 0.1% Chlorella and 0.1% Laurus. Productive performance, nutrient digestibility, blood biochemistry and faecal composition were monitored, and polyphenolic compounds were analysed in feed, blood, faeces and caecal microbiota using HPLC-DAD. Final body weight (3097 vs. 2909 g) and feed intake (142.7 vs. 145.0 g day⁻¹) did not differ significantly between treatments. However, crude protein digestibility was significantly lower in the supplemented group than in the control group (54.39–47.79% vs. 63.73–62.33%; p < 0.05). Faecal chemical composition differed significantly between groups, particularly for dry matter, which was higher in the supplemented group across sampling times. Polyphenols detected across biological matrices confirmed the bioavailability of selected phytochemicals, with ferulic acid showing the highest stability. Correlation analysis indicated shared metabolic or absorptive pathways among several compounds. Overall, low-dose supplementation with C. vulgaris and L. nobilis appears safe and may support improved digestive physiology and nutrient utilisation without compromising rabbit health. Further research with larger sample sizes and detailed microbiome profiling is needed to clarify metabolic interactions and long-term effects of these nutraceutical strategies. Full article