Search Results (142)

Ship propulsion performance is important for navigating. This study aims to enhance the propulsion performance of a 9500 DWT ship by integrating PBCFs into the hull–rudder–propeller–coupled system. A total of 27 PBCF models with different fin installation angles, radius ratio, and tilt angles are designed in the study. The computational fluid dynamics method is employed and a propeller open-water test is also performed to optimize the PBCF design, which is integrated into different coupled systems. The numerical results show that the PBCFs exhibit differential enhancements of propeller performance across system configurations with their efficiency changing from 4.05% to 2.87%. Moreover, the reliability of ship self-propulsion simulation is mutually validated through the combined BF (body force) and MRF (multi-reference frame) methods. Then, simulations were conducted using these two methods for the self-propulsion of a 9500 DWT ship at three different speeds. Finally, the results from using the MRF method show that the incorporation of PBCFs can reduce delivered power to propeller by 1.32% at different F_r. Full article

Manipulating complex light fields through highly anisotropic scattering medium (HASM) remains a fundamental challenge due to the intricate underlying physics and broad application potential. We introduce a unified theoretical and experimental framework for generating and controlling arbitrarily polarized curved caustic beams using an extended polarization transfer matrix (EPTM) for the first time, enabling intuitive polarization transformation through HASM. The EPTM is experimentally measured via a four-step phase-shifting technique, and its submatrices are independently modulated with tailored caustic phase profiles. This strategy facilitates the creation of diverse high-resolution caustic beams, including Gaussian and vortex types with tunable energy distribution, polarization states, and vorticity. The achievement of polarization transformation through HASM by our approach offers versatile manipulation over optical field properties such as multiple high-resolution caustic beams, angular momentum flux, and polarization, paving the way for enhanced functionality in advanced optical systems. Full article

Coastal regions face escalating challenges, including climate change, rapid urbanisation, ocean pollution, habitat degradation, and nutrient enrichment, which threaten coastal ecosystem health, biodiversity, and human livelihoods. A comprehensive understanding of coastal hydro-environmental processes, encompassing hydrodynamics, sediment transport driven by waves and currents, and biogeochemical dynamics influencing water quality, is essential for sustainable coastal management. This study presents a global systematic review of assessment methods for these processes, focusing on field data collection, laboratory experiments, numerical modelling, and artificial intelligence techniques. A bibliometric analysis was conducted on 165 peer-reviewed articles from Scopus and Web of Science, adhering to PRISMA 2020 guidelines. The findings reveal a significant shift from conventional standalone methods to integrated approaches, with 31.5% of studies combining field data with numerical models and 20% incorporating AI with field data, emphasising the need for real-time data integration and interdisciplinary strategies to enhance model reliability. This study also introduces a novel process–method–time classification framework that functionally aligns various assessment methods with specific coastal processes. However, challenges such as limited long-term datasets, high computational costs, and data resolution constraints persist. By synthesising global research trends and methodological advancements, this study offers critical insights to support more resilient, adaptive, and data-driven coastal management strategies. Full article

Xylanases play a crucial role in bio-transforming sustainable agricultural polymers into xylose-based oligosaccharides, which have great potential in various biotechnology applications. Nevertheless, the application of bacterial xylanase is hindered by the high cost of developing recombinant bacteria to overcome the low activity and narrow pH stability. Considerable efforts have been made to discover and explore new wild bacterial strains that produce highly effective and environmentally sustainable extracellular xylanase enzymes for various targeted biotechnological and industrial applications. Lactic acid bacteria (LAB) have recently been proven to be versatile producers of extracellular hydrolytic enzymes. Therefore, this study aimed to isolate and characterise extracellular xylanase-producing LAB (EXLAB) from plant sources. The specific extracellular xylanase activity was determined across a wide pH range, from acidic to alkaline. Subsequently, the expression of xylanase genes of EXLAB grown under acidic and alkaline conditions was determined by quantitative reverse transcription polymerase chain reaction. A total of 45 putative LAB were isolated from radish, gundelia and rhubarb plants. They were identified by phenotypic and genotypic approaches. However, only 15 LAB isolates were confirmed as EXLAB. Weissella confusa and Pediococcus pentosaceus were the most common species among the identified EXLAB. The XylW (~196 bp) and XylP (189 bp) xylanase genes were then amplified from W. confusa and P. pentosaceus, respectively. P. pentosaceus G4 demonstrated the most versatile extracellular xylanase production that was active from pH 5 to pH 8. However, a significant increase in extracellular xylanase gene expression (13.45-fold) at pH 5 was noted as compared to pH 8. Similarly, P. pentosaceus G4 also exhibited the highest extracellular xylanase activity (0.88 U/mg) at pH 5. This study reveals the potential of P. pentosaceus G4 as an eco-friendly and novel extracellular xylanase producer possessing broad pH stability. The robust gene expression and activity of extracellular xylanase imply P. pentosaceus G4 is a promising candidate for sustainable enzymatic processes essential for the environmentally friendly enzymatic reactions and applications. Full article

Background/Objectives: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot and mouth disease (HFMD), often co-circulating and occasionally undergoing genetic recombination. While natural recombinants often involve genomic regions encoding non-structural proteins, their effects on replication and pathogenesis remain unclear. Methods: To address this, four chimera viruses (Chi-CCE, Chi-ECE, Chi-EEC, and Chi-CEC) were constructed with 5′UTR, capsid P1, and non-structural P2 and P3 genes, from CVA16 (denoted as C) or EV-A71 (denoted as E). These chimeras were tested for replication kinetics and cytopathic effects in rhabdomyosarcoma cells while in vivo virulence and protection efficacy were evaluated using a newborn BALB/c mouse model. Results: All chimeric viruses remained viable and exhibited higher replication than CVA16. In vivo, all chimeric viruses were avirulent except Chi-CCE and CVA16, which showed high virulence and viral titres in the brains and limbs of infected newborn mice. This suggests that 5′UTR and capsid P1 genes of CVA16 are critical genetic determinants of virulence. Notably, only the anti-inflammatory cytokine IL-10 was elevated, suggesting potential immune modulation during infection. Inactivated Chi-CCE immunisation conferred 100% protection against lethal CVA16 or mouse-adapted EV-A71 challenge revealing its potential as a bivalent vaccine candidate. Conclusions: Our study demonstrates that recombination between CVA16 and EV-A71 influences viral virulence and protection efficacy with implications for future development of multivalent vaccines. Full article

Xylanase is an essential industrial enzyme for degrading plant biomass, pulp and paper, textiles, bio-scouring, food, animal feed, biorefinery, chemicals, and pharmaceutical industries. Despite its significant industrial importance, the extensive application of xylanase is hampered by high production costs and concerns regarding the safety of xylanase-producing microorganisms. The utilisation of renewable polymers for enzyme production is becoming a cost-effective alternative. Among the prospective candidates, non-pathogenic lactic acid bacteria (LAB) are promising for safe and eco-friendly applications. Our investigation revealed that Pediococcus pentosaceus G4, isolated from plant sources, is a notable producer of extracellular xylanase. Improving the production of extracellular xylanase is crucial for viable industrial applications. Therefore, the current study investigated the impact of various medium components and optimised the selected medium composition for extracellular xylanase production of P. pentosaceus G4 using Plackett–Burman Design (PBD) and Central Composite Design (CCD) statistical approaches. According to BPD analysis, 8 out of the 19 investigated factors (glucose, almond shell, peanut shell, walnut shell, malt extract, xylan, urea, and magnesium sulphate) demonstrated significant positive effects on extracellular xylanase production of P. pentosaceus G4. Among them, glucose, almond shells, peanut shells, urea, and magnesium sulphate were identified as the main medium components that significantly (p < 0.05) influenced the production of extracellular xylanase of P. pentosaceus G4. The optimal concentrations of glucose, almond shells, peanut shells, urea, and magnesium sulphate, as determined via CCD, were 26.87 g/L, 16 g/L, 30 g/L, 2.85 g/L, and 0.10 g/L, respectively. The optimised concentrations resulted in extracellular xylanase activity of 2.765 U/mg, which was similar to the predicted extracellular xylanase activity of 2.737 U/mg. The CCD-optimised medium yielded a 3.13-fold enhancement in specific extracellular xylanase activity and a 7.99-fold decrease in production costs compared to the commercial de Man, Rogosa and Sharpe medium, implying that the CCD-optimised medium is a cost-effective medium for extracellular xylanase production of P. pentosaceus G4. Moreover, this study demonstrated a positive correlation between extracellular xylanase production, growth, lactic acid production and the amount of sugar utilised, implying the multifaceted interactions of the physiological variables affecting extracellular xylanase production in P. pentosaceus G4. In conclusion, statistical methods are effective in rapidly assessing and optimising the medium composition to enhance extracellular xylanase production of P. pentosaceus G4. Furthermore, the findings of this study highlighted the potential of using LAB as a cost-effective producer of extracellular xylanase enzymes using optimised renewable polymers, offering insights into the future use of LAB in producing hemicellulolytic enzymes. Full article

In this study, edge-oxidized graphene oxide (EOGO) was used as an additive in fly ash (FA) geopolymer paste. The effect of EOGO on the properties of the fly ash geopolymer was investigated. EOGO was added to the FA geopolymer at four different percentages (0%, 0.1%, 0.5% and 1%), and the mixture was cured under two different conditions: room curing (~20 °C) and heat curing (~60 °C). To characterize the FA-EOGO geopolymer, multiple laboratory tests were employed, including compressive strength, Free-Free Resonance Column (FFRC), density, water absorption, and setting tests. The FFRC test was used to evaluate the stiffness at small strain (Young’s modulus) via the resonance of the specimen. The mechanical test results showed that the strength and elastic modulus were high during heat curing, and the highest compressive strength and elastic modulus were achieved at 0.1% EOGO. In the physical test, 0.1% EOGO had the highest density and the lowest porosity and water absorption. As a result of the setting time test, as the EOGO content increased, the setting time was shortened. It is concluded that the optimum proportion of EOGO is 0.1% in FA geopolymer paste. Full article

Salient object detection (SOD) plays a critical role in underwater exploration systems. Traditional SOD approaches encounter notable constraints in underwater image analysis, primarily stemming from light scattering and absorption effects induced by suspended particulate matter in complex underwater environments. In this work, we propose a deep learning-based multimodal method guided by multi-polarization parameters that integrates polarization de-scattering mechanisms with the powerful feature learning capability of neural networks to achieve adaptive multi-target SOD in an underwater turbid scattering environment. The proposed polarization-enhanced salient object detection network (PESODNet) employs a multi-polarization-parameter-guided, material-aware attention mechanism and a contrastive feature calibration unit, significantly enhancing its multi-material, multi-target detection capabilities in underwater scattering environments. The experimental results confirm that the proposed method achieves substantial performance improvements in multi-target underwater SOD tasks, outperforming state-of-the-art models of salient object detection in detection accuracy. Full article

Background and Objectives: The role of biventricular pacing (BVP) is less well-established in patients with heart failure with reduced ejection fraction (HFrEF) without left bundle branch block (LBBB). Conduction system pacing (CSP) has gained significant traction and may provide a safe and more physiological alternative to BVP in these patients. A few small studies studying this question have reported conflicting results. This meta-analysis aims to compare procedural and clinical outcomes between CSP and BVP in this group. Materials and Methods: An online literature search was systematically conducted to retrieve studies comparing CSP and BVP in HFrEF patients with non-LBBB. Four studies with 461 patients were included. Results: Implant-derived paced QRS duration was significantly shorter (mean difference [MD] −19.7 ms, 95% confidence interval [CI] −36.2 to −3.3, p = 0.0355) with CSP. Echocardiographic response with significantly greater improvement in left ventricular ejection fraction (MD 5.6%, 95% CI 3.1 to 8.0, p = 0.0106) was also observed with CSP. There were no statistically significant differences in clinical outcomes such as all-cause mortality (relative risk [RR] 0.53, 95% CI 0.18 to 1.60, p = 0.133) and heart failure hospitalization (RR 0.54, 95% CI 0.19 to 1.56, p = 0.129). Conclusions: This meta-analysis suggests that CSP may have better electrical synchrony and echocardiographic response compared to BVP in HFrEF patients with non-LBBB. Further randomized studies with longer follow-up may be required to elucidate potential benefits in clinical outcomes. Full article

Conduction system pacing started with His bundle pacing (HBP) and then rapidly switched gears into left bundle branch pacing (LBBP). We describe our center’s experience with LBBP using either lumenless leads (LLLs) or stylet-driven leads (SDLs). Patients who were admitted to two tertiary centers between 1 April 2021 and 30 June 2024 and met the guidelines for pacing were recruited and prospectively followed up. A total of 124 patients underwent permanent pacemaker (PPM) implantation using the LBBP technique with a mean follow-up of 19.7 ± 13.3 months. In total, 90 patients were implanted with LLLs and 34 with SDLs. There was no significant difference in the procedural time and final paced QRS duration, but fluoroscopy time was significantly longer in the SDLs (26.2 ± 17.7 min vs. 17.5 ± 13.0 min, respectively, p = 0.026). The on-table impedance values were also significantly higher in the LLLs, and this persisted throughout the follow-up. There were no differences in the rates of complications. The success of conduction system pacing implantation with SDLs and LLLs is comparable with reasonable safety and reliable outcomes. Good pre-implant patient selection will contribute to improved outcomes. Full article

Imaging technologies based on vector optical fields hold significant potential in the biomedical field, particularly for non-invasive scattering imaging of anisotropic biological tissues. However, the dynamic and anisotropic nature of biological tissues poses severe challenges to the propagation and reconstruction of vector optical fields due to light scattering. To address this, we propose a deep learning-based polarization-resolved restoration method aimed at achieving the efficient and accurate imaging reconstruction from speckle patterns generated after passing through anisotropic and dynamic time-varying biological scattering media. By innovatively leveraging the two orthogonal polarization components of vector optical fields, our approach significantly enhances the robustness of imaging reconstruction in dynamic and anisotropic biological scattering media, benefiting from the additional information dimension of vectorial optical fields and the powerful learning capacity of a deep neural network. For the first time, a hybrid network model is designed that integrates convolutional neural networks (CNN) with a Transformer architecture for capturing local and global features of a speckle image, enabling adaptive vectorial restoration of dynamically time-varying speckle patterns. The experimental results demonstrate that the model exhibits excellent robustness and generalization capabilities in reconstructing the two orthogonal polarization components from dynamic speckle patterns behind anisotropic biological media. This study not only provides an efficient solution for scattering imaging of dynamic anisotropic biological tissues but also advances the application of vector optical fields in dynamic scattering environments through the integration of deep learning and optical technologies. Full article

Clean agent fire suppression systems are commonly used to protect areas containing valuable or critical equipment, especially in data centers and electrical substations, where traditional fire suppression methods are less effective or pose additional risks. This review evaluates the IG-541 fire suppression system as an alternative to halocarbon-based agents like HFC-227ea and FK-5-1-12, which are being phased out under environmental regulations, focusing on their application in energized electrical fires. IG-541 offers environmental advantages, including zero ozone depletion potential, no global warming potential, and negligible atmospheric lifetime, making it compliant with stringent environmental regulations. This review compares IG-541 with halocarbon agents across parameters such as extinguishing efficacy, safety considerations, environmental impacts, cost impacts, and system design considerations. Key findings underscore IG-541’s effectiveness in reducing fire damage without producing harmful by-products or exacerbating climate change. Furthermore, the study highlights the regulatory frameworks influencing the phase-out of halocarbon agents and the transition toward environmentally sustainable alternatives. While IG-541 emerges as a promising replacement for halocarbon agents, further exploration into its application in varied fire scenarios and energy-intensive environments is recommended to optimize its deployment. Full article

Recurrent fusions drive the pathogenesis of many hematological malignancies. Compared to routine cytogenetic/fluorescence in situ hybridization (FISH) studies, the RNA-based next-generation sequencing (NGS) fusion assay enables the identification of both known and novel fusions. In many cases, these recurrent fusions are crucial for diagnosis and are associated with prognosis, relapse prediction, and therapeutic options. The aim of this study is to investigate the application of the RNA-based NGS fusion assay in hematological malignancies. Our study included 3101 cases with available fusion results, and a fusion event was identified in 17.6% of cases. The discordant rate between the RNA-based NGS fusion assay and cytogenetic/FISH studies was 36.3%. Further analysis of discordant cases indicated that, compared to cytogenetic/FISH studies, the RNA-based NGS fusion assay significantly improved the identification of cryptic fusion genes, such as NUP98::NSD1, P2RY8::CRLF2, and KMT2A fusions involving different partners. Additionally, our study identified 24 novel fusions and 16 cases with the simultaneous presence of two fusions. These additional findings from the RNA-based NGS fusion assay resulted in improved risk stratification, disease targeting and monitoring. In conclusion, our study demonstrates the feasibility and utility of an RNA-based NGS fusion assay for patients with hematological malignancies, suggesting that it may be essential for the routine clinical workup of these patients. Full article

Regional differences in pulsed field ablation (PFA) adoption for pulmonary vein isolation (PVI) with additional posterior wall ablation (PWA) in Asia remains unknown. We hereby report our experience on the safety and efficacy of PFA in AF ablation. Consecutive AF patients who underwent PFA from September 2022 to January 2024 were included. The primary efficacy endpoint was freedom from atrial arrhythmia recurrence after a 90-day blanking period at 12 months. Safety endpoints included 30 days of all-cause death, cardiac tamponade, stroke, myocardial infarction, and heart failure hospitalization. One hundred and one (72.3% males, 79.2% pAF) patients underwent PFA for AF. Thirty-one (30.7%) had structural heart disease with mean LVEF of 57.4 ± 8.1% and CHA2DS2-VASc score of 1.4 ± 1.3. Twenty-nine (28.7%) underwent additional PWA (PVI + PWA) using PFA. PWA was acutely successful in all patients. Patients who underwent PWA were more likely to have persistent AF and require general anesthesia and electroanatomic mapping (all p < 0.05). Total PFA applications for PVI, LA dwell time, procedural time, and fluoroscopy time were similar between the PVI-only and PVI + PWA groups (all p > 0.05). The 1-year atrial arrhythmia recurrence rates were 10% for pAF and 21% for the persistent AF group. The primary efficacy endpoint was not significantly different between the PVI-only and PVI+PWA groups (12-month KM estimates 90.3% [95% CI, 83.3–97.3] and 82.8% [95% CI, 68.1–97.4], respectively). There were no complications related to PFA use. PFA can be safely, effectively, and efficiently adopted for AF ablation. Additional PWA, if pursued, had similar procedural metrics to the PVI-only strategy without increased complications. Full article