Search Results (2,732)

RNA origami enables genetically encoded, single-stranded RNA nanostructures that can self-assemble through co-transcriptional folding and are increasingly deployed as scaffolds for biosensing, synthetic biology, and nanomedicine. A recurring practical bottleneck is scalable, solution-phase readout of whether a designed scaffold has reached its intended base-paired architecture, whether it undergoes slow maturation or kinetic trapping, and how its stability is distributed across motifs. Here, we adapt microfluidic modulation spectroscopy (MMS) as a label-free structural biosensor for RNA folding by exploiting the rich 1760–1600 cm⁻¹ vibrational fingerprints of RNA bases and base pairs. MMS alternates between sample and composition-matched buffer measurements in a microfluidic transmission cell to automatically subtract the solvent background, enabling high-quality spectral measurement from microliter volumes under native solution conditions. Using a six-helix-bundle-with-clasp (6HBC) RNA origami as a model, we established an analysis workflow (baselined second derivative and constrained deconvolution) to quantify paired versus unpaired populations. Thermal ramping resolves multiple unfolding events and yields an unfolding barcode that differs between young and mature ensembles. Importantly, MMS tracks post-transcriptional maturation from a kinetically trapped young conformer toward a more compact, base-paired mature state, consistent with prior cryo-EM/SAXS observations for 6HBC RNA origami. Together, these results position MMS as a rapid, automated, and scalable complement to high-resolution structure determination for engineering dynamic RNA origami biosensors. Full article

Background: Influenza B virus (IBV) undergoes continuous genetic mutations that can affect vaccine effectiveness and immune evasion. Although considerable research on IBV epidemiology exists globally, understanding of its genetic behavior in Saudi Arabia remains limited. This study characterized the molecular epidemiology of IBV in Riyadh, Saudi Arabia, during the 2024–2025 influenza season and evaluated compatibility with the current vaccine strain. Methods: Nasopharyngeal samples (n = 363) were collected from individuals presenting with influenza-like illness at King Khalid University Hospital in Riyadh. Detection and subtyping of IBV were performed using RT-PCR. Complete sequencing of the hemagglutinin (HA) and neuraminidase (NA) genes was conducted on confirmed IBV isolates (n = 7), followed by phylogenetic analysis, amino acid substitution mapping, and glycosylation site prediction. Results: Of the 363 samples analyzed, 68 (18.7%) tested positive for IBV, with the majority occurring in adult females aged 15–64 years. Phylogenetic analysis revealed that all seven IBV isolates belonged to the Victoria lineage under subclade V1A.3a.2, corresponding to the current vaccine strain and strains from the 2022–2023 epidemic season. However, molecular analysis identified two substitutions (D129N and D197E) located in antigenic loop-150 and 190-helix, respectively, in the HA polypeptide that distinguished our strains from vaccine strain B/Austria/1359417/2021. Importantly, the N-glycosylation site at position 169 (NKT), which was present in B/Riyadh/1/2010, has been lost in the IBV strains circulating during 2020–2025. Conclusions: While phylogenetic clade compatibility indicates potential vaccine efficacy, the identified amino acid variations and loss of the glycosylation site underscore the necessity for ongoing molecular surveillance to monitor antigenic changes and evaluate vaccine effectiveness within the Saudi Arabian population. Full article

In the framework of Harmony, the 10th ESA Earth Explorer mission, this paper presents a general methodology to optimize the formation parameters relevant to the single-pass, cross-track interferometry (XTI) configuration. The proposed method considers the requested height sensitivity and the maximum allowable temporal lag and derives the formation parameters for an optimal coverage over different ranges of latitudes by leveraging the relative eccentricity and inclination vector formalism. Our approach addresses the problem of interferometric coherence through the wavenumber support alignment method which is able to take into account the specific geometry of XTI in Harmony, which is a long-baseline multistatic configuration with large squint angles. The analysis is completed by an estimate of the propellant budget, required to maintain the optimized formation, which can be used as a further trade-off parameter within the mission design process. The results indicate that the passively stable helix configuration (with relative eccentricity and inclination phase angles set to 90°) provides a robust solution at equatorial and mid-latitude regions with perpendicular baselines up to the order of 1 km and temporal lag below 10 ms. Conversely, for high-latitude and polar regions, two alternative strategies are identified, revealing a trade-off between enhanced interferometric performance and increased formation maintenance requirements. For polar regions, a first strategy adopts relative eccentric and phase angles of 10°, achieving satisfactory performance across most latitudes, whereas an alternative approach retains the value of 90° and optimizes the formation specifically for high latitudes. These two options result in distinct station-keeping demands since the former strategy requires a $\Delta \mathrm{V}$ budget about two orders of magnitude higher, while the latter remains within a $\Delta \mathrm{V}$ range that is typical for missions of the considered class. Full article

Here we perform a detailed information-theoretic (IT) analysis of atomic electron densities in the periodic table, from hydrogen (Z = 1) to lawrencium (Z = 103). By use of the Shannon entropy, the Fisher information and the disequilibrium functionals in both position and momentum spaces as fundamental descriptors of the atomic densities, the periodic table can be represented in a three-dimensional information space as a continuous, highly ordered manifold. The analysis shows that chemical periodicity naturally emerges as a helicoidal manifold (reminiscent of a helix) at the coordinates of a 3D theoretic-information space (Shannon, Fisher, Disequilibrium), with each period forming one segment within the continuous global trajectory. We find information-theoretic signatures of shell structure, sub-shell filling, and electron-configuration anomalies, such as the familiar irregularities seen in chromium and copper. Therefore, the helicoidal character emerges naturally and is not imposed a priori. Further, through the uncertainty principle of the complementary analysis in momentum space, more insights are gained by exposing maximal information-theoretic differentiation for lighter atoms and compression among heavy elements. Notably, momentum-space analysis reveals that hydrogen occupies a natural intermediate position between helium and lithium based on kinetic energy distribution—contrasting with IT position-space results that emphasize hydrogen’s unique delocalized electron density. Indeed, the 3D IT representation of the elements in position space aligns with the view that H does not belong to either the alkali metals or the halogens, but rather stands as a unique, standalone element. This complementary perspective provides new quantitative support for understanding hydrogen’s dual chemical nature, providing new quantitative insight into ongoing debates about hydrogen’s optimal periodic table position. Furthermore, by considering triadic relationships and complexity properties in relation to the López–Mancini–Ruiz (LMC) and Fisher–Shannon (FS) functionals, we show that atomic complexity increases monotonically along with nuclear charge, and we provide a quantitative measure of how organized atomic electron densities are distributed throughout the periodic system. Based on our IT analyses, the fundamental character of periodicity could be addressed by employing helicoidal representations that highlight the characteristics of hydrogen, while simultaneously preserving the autonomy of the blocks of elements. Full article

To address the poor cutting stability and deterioration of hole quality caused by the inherent trade-off between chip evacuation performance and drill-body stiffness in U-drilling, a multi-objective optimization framework was established. The design variables were the core thicknesses ${\mathrm{L}}_1$ and ${\mathrm{L}}_2$ of the inner and outer chip flutes, the inner and outer offset angles ${\mathrm{\theta }}_1$ and ${\mathrm{\theta }}_2$, and the inner and outer helix angles ${\mathrm{\beta }}_1$ and ${\mathrm{\beta }}_2$. The objectives were to maximize the chip evacuation force and minimize the drill-body strain (which serves as an equivalent indicator of maximizing drill-body stiffness). The chip evacuation force was rapidly evaluated using a mechanistic chip evacuation force model derived from mechanism-based analysis. The drill-body strain was efficiently predicted using a GA–BP neural-network surrogate model. An NSGA-II algorithm combined with the entropy-weighted TOPSIS method was employed to solve the optimization problem, yielding the optimal parameter combination for the U-drill chip-flute geometry. The results show that drilling experiments on 42CrMo under the optimal structural parameter combination reduced the cutting forces in the $x$, $y$, and $z$ directions by approximately 11.2%, 13.1%, and 11.8%, respectively. The root-mean-square acceleration in the $x$ and $y$-directions decreased by about 17.3% and 22.9%, respectively. These improvements effectively enhanced the hole-wall surface roughness and hole diameter accuracy, and further improved chip evacuation smoothness and cutting stability of the U-drill. Full article

To address the complex dynamic mechanisms and lack of static operation data in trench-digging for transverse planting of Salix psammophila sand barriers, a transverse trench-digging device was designed. Based on the discrete element method, the Hertz–Mindlin with JKR Cohesion model was used to simulate sandy soil. The Box–Behnken experiment was adopted to optimize the single auger structure with helix angle and soil-cutting angle as factors and trench depth and working torque as indices, yielding the optimal parameters of 30° soil-cutting angle and 20.37° helix angle (5.52 cm trench depth, 2.6 N·m maximum torque). The optimized auger was integrated into the device, and a further Box–Behnken experiment was conducted under a 20 cm fixed descending depth of the lifting platform. With auger rotation speed, shaft spacing and lifting speed as factors, and trench depth, soil compaction and Salix psammophila insertion depth as indices, the optimal operating parameters were determined as 257.25 r/min, 7 cm and 9 cm/s, corresponding to 6.7 cm trench depth, 33.37 kPa soil compaction and 14.87 cm insertion depth. This study clarifies the effects of auger and operation parameters on trench-digging quality, provides a basis for the design and parameter matching of dynamic continuous operation equipment, and offers a reference for the R&D of mechanized transverse planting equipment for Salix psammophila sand barriers, which is of practical value for reducing sand control costs and improving efficiency. Full article

The replacement of petroleum-based plastics with sustainable and biodegradable materials remains a critical challenge for food packaging and biomedical applications. Gelatin is an attractive natural biopolymer for film fabrication; however, its inherent brittleness, moisture sensitivity, and limited structural stability restrict practical use. In this work, for the first time, low-power direct-probe ultrasonication is introduced as a green and additive-free strategy to regulate molecular organization and enhance the performance of gelatin–glycerol composite films. Systematic variation in ultrasonic power and treatment duration revealed a strong dependence of film structure and properties on processing conditions. Low-power ultrasonication (20 W) promoted gelatin–glycerol interactions, induced a transition from loosely organized molecular arrangements to helix-like molecular packing at the nanometer scale, and produced smooth, compact microscale surface morphologies. As a result, these films exhibited enhanced hydrophilicity, reduced surface defects, and improved thermal stability. In contrast, high-power ultrasonication generated excessive cavitation, leading to large-scale porous structures and diminished thermal and surface performance. Therefore, this work identifies a distinct low-power ultrasonic window that enables controlled molecular reorganization and hierarchical structure formation in gelatin–glycerol systems. Structural and physicochemical analyses using SEM, FTIR, XRD, water contact angle measurements, and thermogravimetric analysis collectively elucidate the ultrasound-driven structure–property relationships within the gelatin–glycerol matrix. Overall, this study demonstrates that controlled ultrasonication enables precise tuning of gelatin-based film architecture and properties, offering a scalable and environmentally friendly route to high-performance biodegradable materials for sustainable packaging and biomedical applications. Full article

Vitamin D signaling via the vitamin D receptor (VDR) regulates calcium–phosphate homeostasis and extensive gene programs controlling cell proliferation, differentiation, immune tone, and metabolism. However, systemic use of the natural agonist 1α,25-dihydroxyvitamin D₃ (calcitriol) for extraskeletal indications is limited by dose-limiting hypercalcemia. This review summarizes VDR biology and the structural basis of ligand action, emphasizing how ligand-induced repositioning of helix 12 and altered coregulator recruitment can be exploited to engineer selective VDR modulators. We highlight medicinal chemistry strategies spanning secosteroidal analogs with side-chain or ring modifications and emerging non-seco scaffolds and discuss clinically established agents (e.g., calcipotriol and paricalcitol) alongside experimental “super-agonists”, partial agonists, and antagonists designed to widen the therapeutic window. Finally, we discuss current evidence for VDR targeting across cancer, metabolic disease, fibrosis, and immune-inflammatory disorders, including mechanisms of resistance such as dysregulated vitamin D metabolism and epigenetic repression. Structural and epigenomic insights are positioning next-generation VDR ligands as tissue- and pathway-biased therapeutics that may enable safer, mechanism-guided translation beyond bone and mineral indications. Full article

This integrative literature review synthesises five strands of recent scholarships on port city regions (PCRs): (1) their multidimensional transitions, (2) skills foresight and future competences, (3) challenges and reforms in vocational education and training (VET) systems, (4) social inclusion and equity in skills development, and (5) innovative VET methodologies in port-adjacent sectors. Drawing on the interdisciplinary academic and policy-oriented literature, this article adopts a qualitative, integrative review approach to examine how the twin green and digital transition is reshaping port city regions and their associated skills ecosystems. The review demonstrates that PCR transitions are not only technical but socio-institutional: while Onshore Power Supplies (OPSs), alternative fuels, and digital platforms are transforming operational landscapes, the success of these innovations depends critically on the adaptive capacity of workers, training systems, and governance arrangements. The article further examines emerging pedagogical approaches in port-adjacent VET, including work-based learning, micro-credentials, and immersive training methods. Taken together, the evidence converges on a central claim: the resilience and sustainability of port city regions depend on integrated skills systems that combine foresight, inclusivity, and pedagogical innovation. Without such systems, decarbonisation and digitalisation risk exacerbating social and spatial inequalities rather than fostering sustainable growth. The article concludes by outlining implications for research, policy, and practice, calling for integrative performance metrics, longitudinal evaluation, and quadruple helix collaboration to support inclusive, competitive, and sustainable port transitions. Full article

In nature, organisms have evolved unique structures that feature low weight, high strength, and damage resistance. The Eurasian eagle-owl serves as a representative example, with specialized feather architectures that enable stable flight in intense and turbulent airflow conditions. Herein, driven by classical design layup guidelines, and inspired by the distinctive fiber architecture of the feather shaft cortex, we propose a regionally tailored layup (RTL) design to enable mass-efficient composite beams with high load-bearing capacity and enhanced damage tolerance. The feather shaft reference lay-up rectangular beam (FSRB) adopts the RTL, and a flange overlap is introduced to preserve the integrity and strength of the flange–web interface; it is then manufactured using inner–outer matched molds in conjunction with vacuum bag molding. Three-point bending shows that the FSRB achieves a flexural strength of 180 MPa and a flexural modulus of 12.1 GPa. Relative to conventional axial (ALRB), Cross-ply (CPRB), single-helix (SLRB), and quasi-isotropic (QLRB) lay-up rectangular beams, the FSRB improves strength by 59.5%, 46.6%, 26.8%, and 21.2%, and increases modulus by 81.7%, 34.7%, 25.1%, and 10.8%, respectively. FEA and SEM observations confirm an RTL architecture in the rectangular beams, characterized by differentiated fiber arrangements in the flange and web. Flanges with an axially dominated layup provide high initial flexural strength and stiffness. The web, formed by a crossed-ply/axial hybrid layup, provides transverse support and redirects crack/delamination growth, thereby promoting progressive failure and enhancing energy dissipation. Overall, this RTL design enables concurrent improvements in load-carrying capacity and damage tolerance. This study offers a design perspective for high-performance load-bearing components. Full article

The transition to circular models in fashion and textiles requires changes that go beyond technical innovation. The literature recognises that systemic change depends on the transformation of shared meanings around consumption and production, and that spaces for co-design and collaborative learning are crucial to generating this transformation. This article documents how Living Labs operate in this capacity, analysing the Madeback Circular Fashion Festival (May–November 2025), a pilot project of the Fashion & Textile Living Lab at the Politecnico di Milano. The study employs the Living Lab Integrative Process (LLIP) as both a design framework and an analytical lens. Adopting a qualitative and participatory method, the study documents how the three spaces of the LLIP—Problem Space, Solution Space and Implementation Space—simultaneously structured both design innovation and empirical analysis. The results point to three main contributions: (i) Living Labs can function as cultural infrastructures in which performative and narrative dimensions may contribute to the gradual normalisation of alternative practices; (ii) the Quadruple Helix operates as a living process characterised by distributed intentionality and emerging trust; and (iii) transformative learning appears through the co-production of knowledge in embodied and relational practices. The article identifies contextual factors that enabled the project—from its location in a design university to its multi-year funding—and the related constraints on transferability, concluding that Living Labs are promising infrastructures for sustainable transitions when they consciously integrate performative, cultural and relational dimensions. Full article

This review summarizes scientific advances about the sonochemical synthesis of starch nanoparticles (St-NPs) for the food industry, as well as nutraceutical and drug delivery applications. High-intensity ultrasonication (HIU) has been explored as a versatile and environmentally friendly alternative to conventional methods for synthesizing St-NPs with high yields (>90%), controlled size (~100 nm), and minimal effluent generation. Thus, HIU has been explored (pre- or post-treatment) to mitigate the inherent disadvantages (high-cost, low yields, and environmental impact) of hydrothermal gelatinization, acid/alkaline hydrolysis, enzymatic hydrolysis, enzyme branching, water-in-oil and oil-in-water emulsions, non-solvent nanoprecipitation, extrusion, high-pressure homogenization, high-energy milling, and cold plasma. Conventional sources of starch (corn [normal, waxy, high-amylose] and potato) and other unconventional sources (tubers [cassava, yam, malanga], seeds and grains [sorghum, barley, quinoa, lotus], breadfruit, pinhao seed, Araucaria angustifolia) have been subjected to single or assisted sonochemical protocols to obtain St-NPS with unique structural, physicochemical, and technological properties. The physical–mechanical effects of ultrasonication (cavitation, heat, and pressure) directly promote surface functionalization (i.e., esterification, pore formation) and impact the St-NPS’s particle size, double-helix structure, enzymatic-resistance properties, crystallinity, and intra- and intermolecular arrangements. Pickering additives in food systems, colloids in beverages, nanocomposites in biofilms for food packaging, and nanocarriers for drug and nutraceutical delivery (oral and transdermal) have been the most reported applications. Full article

Collagen is the primary protein in the extracellular matrix of human cells and the body and is essential for cell structure and function. Here, for the first time, we report a method for producing recombinant triple-helical collagen type III (rhCOL3) in transgenic tobacco as a bioreactor. We constructed a pMDV-COL3A1 vector containing the human type III collagen gene COL3A1, as well as a pMDV-COL3A1:5E vector that coexpressed COL3A1 and the enzymes required for its posttranslational modification. These two vectors were used to transform tobacco genetically. The COL3A1 gene was successfully coexpressed in tobacco plants with four enzymes that promote its posttranslational modification. The transcriptional level of COL3A1 in the transgenic lines coexpressing posttranslational modification genes was greater than that in the transgenic lines expressing only COL3A1. The enzyme-modified recombinant collagen was subsequently purified from a COL3A1:5E transgenic line. Our experimental results demonstrated that the terminal propeptides of plant-derived rhCOL3 can be correctly cleaved through the enzymatic hydrolysis of procollagen by coexpressed procollagen C proteinase (PCP) and procollagen N proteinase (PNP). The plant-derived rhCOL3 was thermally stable because the purified peptide chains can form a triple helix structure. Experiments have shown that plant-derived rhCOL3 has biological activity. In this study, functional recombinant full-length mature type III collagen with a triple-helix structure was successfully expressed in tobacco, providing a foundational plant-made material for future applications of collagen in human skin and bone repair in regenerative medicine. Full article

The effects of storage time on the characteristics of starch in spicy strips were investigated. Techniques including differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) were employed to analyze the gelatinization properties, thermal characteristics, crystal structure, moisture distribution, and quality changes of spicy strips under different storage periods (0, 60, 120, and 180 days). The results demonstrated that prolonged storage led to a significant decrease in peak viscosity and an increase in setback value, indicating enhanced starch retrogradation. DSC analysis revealed a continuous increase in enthalpy change (ΔH), confirming the formation of more ordered double-helix structures over time. TGA revealed a shift in thermal degradation profiles, indicating changes in component interactions and moisture-binding capacity over storage. XRD patterns showed a clear transition from A-type to V-type crystals and finally to an amorphous state after 180 days. Consequently, solubility, swelling power, and amylose leaching were markedly inhibited, while the retrogradation rate of amylopectin became dominant during long-term storage. These findings provide insights into starch retrogradation mechanisms in complex snack matrices and offer guidance on mitigating quality deterioration during the shelf life of spicy strips. Full article

Background: Opioid receptors are a commonly used target for treatment of pain conditions. Most opioids used in therapy are linked to adverse effects such as tolerance, dependence, and respiratory depression. Indole alkaloids acting on opioid receptors may provide a novel molecular mechanism to confer analgesic effects. Results: Indole alkaloids such as ibogaine and mitragynine act on μ-opioid receptors as biased full or partial agonists that do not, or much less strongly, recruit β-arrestin compared to non-biased agonists. The recruitment of β-arrestin has been linked to adverse effects, most notably substantial respiratory depression. The molecular mechanism of biased activation has been proposed to be associated with accommodation of the indole structure that leads to a different spatial orientation of amino acid residues in transmembrane regions 2 and 3 of the μ-opioid receptor as well as extracellular helix 8. Conclusions: Naturally occurring indole alkaloids show biased G-protein coupled activation of opioid receptors with limited recruitment of β-arrestin, thus limiting commonly observed adverse effects. Indole alkaloids may present a feasible structure to develop new biased opioid modulators with an improved risk-to-benefit ratio. Full article