Search Results (405)

The increasing deployment of resource-constrained Internet of Things (IoT) devices requires security mechanisms that preserve confidentiality without compromising energy efficiency or responsiveness. Although Transport Layer Security (TLS) provides standardized protection for MQTT-based communication, its computational overhead may significantly affect embedded architectures. This study presents a controlled experimental evaluation of three communication configurations implemented on ESP32-based nodes: unencrypted Message Queuing Telemetry Transport (MQTT), MQTT over TLS 1.2, and an application-layer hybrid scheme combining Elliptic Curve Diffie–Hellman key exchange with AES-128 encryption. Second-level measurements of instantaneous current, accumulated energy, end-to-end latency, and memory footprint were collected across repeated experimental runs. Time-series diagnostics were performed to assess autocorrelation and stationarity, and block bootstrap resampling was applied to ensure dependence-aware statistical inference. The results indicate that TLS introduces the highest cumulative energy growth and latency dispersion, while the hybrid ECC–AES configuration demonstrates intermediate behavior with reduced overhead relative to TLS. Pareto frontier analysis shows that TLS is dominated in the joint energy–latency space, whereas the hybrid scheme represents a non-dominated compromise between security and efficiency. These findings provide a stability-aware and statistically robust framework for evaluating security–performance trade-offs in embedded IoT systems. Full article

Introduction: Healthcare-associated infections are a significant public health challenge, particularly in resource-limited settings. While hand hygiene is critical for infection prevention, contaminated water from hand hygiene stations (HHSs) in healthcare facilities (HCFs) may undermine infection control efforts. Chlorination can reduce microbial contamination in HHSs, ensuring that water intended for hygiene does not become an infection source. Methods: Water quality was monitored before and after the installation of on-site chlorine dispensers (CDs) in water tanks and HHSs of HCFs in Quetzaltenango, Guatemala, to evaluate their effectiveness in improving water quality. Focus groups were conducted to develop action plan proposals to ensure the intervention’s sustainability. Results: Before the intervention, 75% of HHS water samples tested positive for total coliforms, with 50% testing positive for presumptive extended-spectrum beta-lactamase (ESBL)-producing total coliforms, while 20% were E. coli-positive, with 50% presumptive ESBL-producing E. coli. After installing CD, 1% of samples were coliform-positive over a six-month period. Focus groups identified resource limitations and political barriers and proposed solutions such as developing operational manuals, strengthening inter-institutional relationships, and forming alliances with external organizations. Conclusion: Localized chlorination was successfully implemented using a community participatory approach to improve water quality in resource-limited HCFs. These findings have important implications for infection prevention and control. Full article

Yellow fever remains a major public health threat in endemic and re-emerging regions of Africa and South America, with recent outbreaks highlighting persistent gaps in prevention and surveillance. Pregnant women represent a particularly vulnerable population, yet the epidemiology, clinical impact, and preventive strategies for yellow fever in pregnancy are insufficiently characterized. Physiological and immunological changes during gestation may influence host responses to infection; however, current evidence does not demonstrate increased susceptibility to or severity of yellow fever during pregnancy. Adverse materno-fetal outcomes, including miscarriage, stillbirth, preterm birth, and, in rare cases, perinatal transmission, have been reported but remain poorly characterized. Diagnostic challenges, overlapping clinical presentations with other arboviral and hepatic diseases, and limited access to specialized care further complicate clinical management in many endemic settings. This perspective provides a comprehensive overview of yellow fever in pregnancy during the 2024–2026 outbreak in the Americas, including a risk-stratification framework for prevention. We summarize current evidence on epidemiology, pathophysiology, diagnosis, and supportive care, and examine prevention strategies with particular emphasis on vaccination. Accumulated observational evidence and substantial real-world experience have not demonstrated an increased risk of serious adverse events and generally support the effectiveness of yellow fever vaccination during pregnancy when administered with appropriate clinical judgment. In high-risk settings, the benefits of maternal immunization clearly outweigh theoretical concerns, supporting a flexible, risk-based approach, despite relatively limited evidence. We also discuss national and international policies, post-pregnancy booster recommendations, and the importance of integrating vaccination assessment into antenatal care. Finally, we highlight critical knowledge gaps and research priorities, including the need for prospective registries and strengthened pharmacovigilance. Coordinated clinical and public health strategies are essential to protect maternal and neonatal health and to reduce the burden of yellow fever in endemic and re-emerging settings. Full article

The Mam Mayan Christians of Guatemala’s Western Highlands regularly ascend sacred mountains to pray for the precarious migration journey across Mexico and into the United States. This paper describes and explicates the cultural logic connecting mountains, migration, and prayer through an analysis of linguistic practices across three domains: (1) the tacit and habitual reference to mountains in common Mam grammatical form classes, (2) the discourse patterns that link the precarities of migration to mountaintop prayer, and (3) the context for and structure of mountaintop prayer rituals. Taken together, the analysis of these three domains describes a Mam ontology of mountains that render mountaintop prayer the most important venue for facing the precarities of international migration. The paper closes by considering the habitus of Mam Maya Evangelical Christians as a source of Indigenous theological praxis. Full article

The effects of three metabolizable energy (ME) levels and the use of guanidinoacetic acid (GAA) were evaluated on broiler performance and nutrient digestibility from 1 to 35 d of age. In total, 1944-d-old Ross AP95 male broilers were randomly distributed to six treatments (12 replicates/treatment). Diets were formulated to contain three ME levels (standard energy [SE], −50 kcal/kg reduced energy [−50 RE] and −100 kcal/kg reduced energy [−100 RE]) in all feeding phases with or without GAA inclusion. For the nutrient-metabolizable analysis, 960-day-old male broilers were separately raised in floor pens until 14 d of age and randomly distributed to six treatments (16 replicates/treatment). Data were analyzed with ANOVA and Tukey’s test at p ≤ 0.05. There was a significant interaction for the feed conversion ratio (FCR) at 21 days, in which the PC diet showed better FCR when GAA was included. In the evaluation of the main effects, an effect of metabolizable energy (ME) was observed on body condition score (BCS) at 7 and 21 days, feed intake (FI) at 21 and 35 days, and feed conversion ratio (FCR) at 21 days, with the PC diet showing better FCR and lower FI. An effect of GAA was observed on feed conversion ratio at 21 days, with the inclusion of GAA in the diet showing better FCR. In conclusion, broilers fed SE diets with GAA, beyond better performance, had improved AME and AMEn compared to broilers fed RE diets without GAA in starter diets. Full article

Facial cosmetics are undergoing a significant transformation driven by scientific innovation, consumer awareness, and a growing demand for personalized and sustainable solutions. This article explores the latest developments in active ingredients, such as exosomes, growth factors and cosmeceuticals, alongside the rise of smart skincare technologies, including AI-based diagnostics and connected beauty devices. It also examines evolving consumer preferences, with a focus on transparency, skin health, and environmentally conscious formulations. Together, these trends are shaping a new era in facial cosmetic care, where efficacy, safety, and sustainability converge. Full article

West Nile virus (WNV) is a mosquito-borne flavivirus with one of the widest global distributions. Since its discovery in Uganda in 1937, it has become a major zoonotic pathogen, and after its introduction into the United States in 1999, it spread rapidly across the Americas, becoming the leading cause of neuroinvasive arboviral disease. Its expansion illustrates a remarkable ecological adaptability, further intensified by climate change. In Latin America and the Caribbean, WNV circulation has been consistently documented in birds, horses, and mosquitoes; however, confirmed human cases remain disproportionately scarce compared with North America and Europe. Reports include sporadic human cases in Brazil (>100 since 2014), Mexico (~13), Argentina (2006–2007), Puerto Rico (2007), Nicaragua, and Haiti, while animal and vector evidence extends to Guatemala, El Salvador, Belize, Costa Rica, Bolivia, Paraguay, Colombia, Venezuela, Cuba, and Ecuador. This paradox likely reflects structural limitations within regional health systems, including underdiagnosis, restricted diagnostic capacity, and significant surveillance gaps, particularly in contexts where mild febrile syndromes may be misclassified as dengue, Zika, or Chikungunya. The regional risk of emergence is further amplified by climatic variability, ecological change, and intensifying human–wildlife interactions. Experiences from Europe highlight the importance of early detection, transfusion safety, and integrated surveillance within a One Health framework. Strengthening preparedness in Latin America will require investments in diagnostic infrastructure, implementation of standardized seroepidemiological surveys, development of predictive models tailored to local ecological contexts, and robust intersectoral collaboration. Full article

Cardamom (Elettaria cardamomum (L.) Maton) is a major export crop in Guatemala; however, its genetic basis remains largely unexplored. This study aimed to evaluate the genetic diversity and differentiation among 288 cardamom accessions from the Northern Transversal Strip, the country’s primary production area. Eleven molecular markers (SSR, ISSR, and EST-SSR) were used to generate multilocus profiles analyzed under a dominant model. Genetic diversity revealed average values of Shannon’s index (I = 0.316) and expected diversity (h = 0.207), with SSR markers providing the highest values (I = 0.364, h = 0.233). Bayesian and hierarchical analysis identified three genetic groups (K = 3). The relatively low diversity observed is consistent with the introduction history of this crop in Guatemala, human-driven selection, and historical bottlenecks caused by Cardamom Mosaic Virus and thrips infestations. Despite these constraints, private and high-frequency bands were detected across genetic groups, offering potential for marker-assisted selection. These findings provide the first genetic baseline for Guatemalan cardamom, supporting future breeding strategies aimed at improving resilience, productivity, and adaptation to climate change. Full article

This study systematically investigates the mineralogical, spectral, and geochemical characteristics of Guatemalan lavender jadeite and black omphacite to elucidate their coloration mechanisms and genetic origins. Lavender samples are primarily composed of jadeite, which derives its color from synergistic effects involving Mn³⁺ and Fe²⁺-Ti⁴⁺ charge transfer (554–614 nm). In contrast, black samples are dominated by omphacite, which owes its dark hue to Cr³⁺ (670 nm) and Fe²⁺-Fe³⁺ charge transfer (857 nm). Chemically, lavender jadeite exhibits higher Na₂O and Al₂O₃, approaching the jadeite end-member composition, whereas black omphacite is enriched in CaO, MgO, and FeO. Trace element analyses reveal low overall abundances, with black omphacite showing synchronous LREE and HREE depletion forming a “bulge-shaped” pattern, while lavender jadeite displays N-MORB-like REE distributions. Guatemalan jadeites are distinguished from Myanmar counterparts by Y enrichment. The identification of graphite and CH₄ and CO₂ fluid inclusions indicates formation in an organic-rich reducing environment. Cathodoluminescence zoning and abundant fluid inclusions support a direct crystallization genesis from high-pressure fluids (P-type) in subduction zones. This study establishes key constraints for origin discrimination and genetic classification of Guatemalan lavender jadeite and black omphacite. Full article

Land-use and land-cover (LULC) change is one of the most pervasive drivers of socioenvironmental transformation worldwide. Given its impacts on ecosystems and climate, the systematic analysis of LULC dynamics remains a central objective of land-change science. Despite major advances in Earth observation capabilities, robust, flexible, and scalable algorithms for long-term monitoring remain unevenly adopted, particularly in remote, forested tropical regions. This study introduces the Multivariate Random Forest Land-Use and Land-Cover (MuRaF-LULC) framework, a supervised and generalizable framework that produces annual, multi-class LULC maps from Landsat time series, with interannual change derived through year-to-year comparisons. A key methodological component of the framework is its predictor-selection strategy, in which variable-importance rankings are used to identify an optimized subset of predictors prior to final model training. MuRaF-LULC was implemented in Google Earth Engine (GEE) and evaluated in Guatemala’s Maya Biosphere Reserve (MBR) for the 2018–2024 period using probability-based sampling and uncertainty-aware accuracy assessment and area estimation. Results show that MuRaF-LULC generates robust annual LULC classifications across multiple years (overall accuracy = 0.90–0.92) and reliable estimates of agropecuario expansion (the dominant transition in the study area) when change is assessed over longer temporal windows where transitions signals stabilize and for which the framework is best suited (producer’s accuracy = 0.97 ± 0.03; user’s accuracy = 0.69 ± 0.05). By prioritizing consistent annual, multiclass LULC trajectories, MuRaF-LULC complements breakpoint- and disturbance-oriented approaches commonly used in land-change studies. Implemented in publicly available, well-documented GEE scripts, MuRaF-LULC facilitates policy-relevant LULC assessment by remote sensing practitioners in governmental and private organizations, where reproducibility, clarity, and ease of deployment are as important as methodological sophistication. Full article

Background/Objectives: Rural communities in Guatemala face a growing double-burden of malnutrition. Women of reproductive age are a key population to address, as their health and nutritional status influence not only their own well-being but also that of their children and families. However, they often experience greater exposure to nutritional risks due to gendered inequalities in access to resources, education, and health care. This small-scale observational study aimed to describe the dietary habits and nutritional status of a sub-group of women living in a rural area of Bárcena Villa Nueva, Guatemala. Methods: An observational study was conducted between March and April 2025 among women aged ≥18 years from two rural communities. Dietary data were collected through structured interviews, 24 h dietary recall (24-hR), and a validated food frequency questionnaire (FFQ). Anthropometric and biochemical measurements were also collected. Results: A total of 22 women were included (mean age: 41.3 ± 16.3 years). The prevalence of obesity and central obesity was 45.5% and 86.0%, respectively. Quantitative dietary assessment based on the 24 h recall showed a mean energy intake approximately 35% higher than the recommended values, with a high contribution from fats and carbohydrates and an excessive sodium intake. In contrast, intakes of potassium, zinc, and folic acid were below the recommended levels. The qualitative analysis of the food frequency questionnaire indicated a dietary pattern characterized by high consumption of carbohydrates, animal-based protein sources, traditional energy-dense foods, and ultra-processed products, alongside a limited intake of vegetables and fruits. Datary diversity was low (4.9, SD: 1.1). Conclusions: This small-scale observational scale study provides a preliminary overview of dietary patterns and nutritional status among women living in a rural community in Guatemala. Although the findings are not generalizable, they suggest the coexistence of excessive energy intake, suboptimal micronutrient intake, and low dietary diversity. These results underscore the need for further research using larger and more representative samples and may help inform the development of context-specific nutrition education initiatives in similar underserved settings. Full article

Introduction: Gestational diabetes (GD) screening remains limited in many low- and middle-income countries (LMICs) due to resource constraints, limited training, and low community awareness. Although community-centered approaches may improve access to screening in rural and Indigenous settings, the implementation processes through which such approaches are designed and operationalized are rarely documented. Methods: This study presents a community-based implementation process report describing the development, adaptation, and early implementation of a GD screening program in rural Guatemala, guided by the Exploration, Preparation, Implementation, and Sustainment (EPIS) implementation science framework. Using a participatory approach, international screening guidelines were systematically adapted to the local context through iterative protocol refinement, structured stakeholder engagement, and ongoing feedback from community health educators and partner institutions. Aggregate program data were used descriptively to characterize early screening uptake and feasibility. Results: Key implementation challenges included patient no-shows, community skepticism, and difficulties among health educators in interpreting screening procedures. Iterative adaptations were introduced to simplify protocols, reduce loss to follow-up, and strengthen community engagement. Over time, the program expanded from point-of-care screening to more comprehensive prenatal services and increased collaboration with the Ministry of Health and local community outlets. A total of 103 Indigenous Mayan Tz’utujil women were screened (mean age: 26.9 years; range: 15–46), of whom, 12 were diagnosed with GD. Conclusions: This implementation process report demonstrates the scientific value of systematically documenting real-world adaptation, feasibility, and stakeholder engagement when introducing GD screening in rural Indigenous LMIC settings. The implementation lessons described may inform similar maternal health initiatives in comparable contexts. Full article

Background: Iron plays a crucial role in neurotransmitter synthesis, myelination, and neuronal metabolism. Iron deficiency has been associated with a variety of neurodevelopmental disorders, particularly attention-deficit/hyperactivity disorder (ADHD) and autism spectrum disorder (ASD). However, the prevalence, clinical impact, and treatment implications differ between these conditions. Objective: To synthesize current evidence on the prevalence, neurobehavioral consequences, and therapeutic implications of iron deficiency in ADHD and ASD, highlighting convergences and disorder-specific findings. Results: In ADHD, studies using serum ferritin and related peripheral markers show inconsistent associations with core symptom severity, with reported ferritin thresholds for deficiency ranging widely. While some studies suggest links between low ferritin and hyperactivity, inattention, or stimulant response, others report null findings. In contrast, emerging neuroimaging evidence consistently demonstrates reduced brain iron in dopaminergic regions in children. In ASD, the strongest link is between low ferritin and sleep-related motor disturbances, and iron supplementation may improve sleep and motor symptoms. Conclusions: Screening for iron status and targeted supplementation may improve sleep and behavioral outcomes in ADHD and ASD, meriting integration into clinical practice and further randomized controlled trials. Full article

Guatemala’s intercountry adoptions were suspended in 2007 after widespread illicit procedures and the persistent trafficking of children. This article is a historical and policy analysis of the related social welfare systems. It uses Midgley’s framework to examine the past and the changes that have resulted in Guatemala’s reform era. Specific attention has been paid to non-formal systems, market-based or profit-oriented systems, non-profit and faith-based systems, and importantly, government-based systems. Previous (pre-reform) child welfare systems, particularly during the millennium adoption surge, are then compared to a relatively new and reformed system. An international child rights legal and policy context, to include the Hague Convention on Intercountry Adoption, frames the discussion that also considers the passage of the 2007 Adoption Law. The article has a child rights perspective and considers the role of women, particularly birth parents, during Guatemala’s peak adoption years. Full article

Background: Ultra-processed food (UPF) consumption has increased significantly in Latin America and Spain, impacting both health and environmental sustainability. To our knowledge, this is the first multicenter study to examine the association between UPF consumption and sustainable lifestyle behaviors in Latin America and Spain. Objective: To evaluate the association between UPF consumption and sustainable lifestyle behaviors in Latin America and Spain. Methods: This was an observational, analytical, multicenter, cross-sectional study. A validated, self-administered online questionnaire was distributed in 14 countries between March 2023 and January 2024. The survey collected sociodemographic data, UPF intake (classified using the NOVA system), body mass index and sustainable lifestyle behaviors (food, transport, environment). Multivariate linear regression models were applied to assess associations, adjusting for age, sex, smoking, physical activity and BMI. Results: Among 6009 adults (mean age: 34.98 ± 12.55; 79.5% women), those with the highest consumption of UPF (fast food, beverages and juices, salty snacks and sweet snacks) were significantly more likely to be in the least sustainable quartile compared to those who did not consume these food products ((OR = 2.51; 95% CI: 1.79–3.54), (OR = 1.82; 95% CI: 1.50–2.22), (OR = 1.51; 95% CI: 1.32–1.73) and (OR = 1.42; 95% CI: 1.20–1.67), respectively, with p values < 0.001). Conclusions: High consumption of ultra-processed foods (UPFs) is inversely associated with sustainable lifestyles. These findings position UPF consumption not only as a health problem but also as a key indicator of unsustainable lifestyles. Full article