Search Results (81)

The accurate short-term forecasting of global horizontal irradiance (GHI) is essential to optimizing the operation and integration of solar energy systems into the power grid. This study evaluates the performance of the Weather Research and Forecasting (WRF) model in predicting GHI over a 48 h forecast horizon at an Italian site: the ENEA Casaccia Research Center, near Rome (central Italy). The instantaneous GHI provided by WRF at model output frequency was post-processed to derive the mean GHI over the preceding hour, consistent with typical energy forecasting requirements. Furthermore, a decomposition model was applied to estimate direct normal irradiance (DNI) and diffuse horizontal irradiance (DHI) from the forecasted GHI. These derived components enable the estimation of solar energy yield for both concentrating solar power (CSP) and photovoltaic (PV) technologies (on tilted surfaces) by accounting for direct, diffuse, and reflected components of solar radiation. Model performance was evaluated against ground-based pyranometer and pyrheliometer measurements by using standard statistical indicators, including RMSE, MBE, and correlation coefficient (r). Results demonstrate that WRF-based forecasts, combined with suitable post-processing and decomposition techniques, can provide reliable 48 h predictions of GHI and DNI at the study site, highlighting the potential of the WRF framework for operational solar energy forecasting in the Mediterranean region. Full article

The global prevalence of antibiotic-resistant bacteria, such as Staphylococcus aureus, presents a substantial challenge to public health, necessitating the development of innovative therapeutic strategies to combat these infections. This study examined the synergistic effects of a biosurfactant (BS) derived from Lactiplantibacillus plantarum and a novel extract from Rosmarinus officinalis (RoME) obtained through supercritical CO₂ extraction against S. aureus sourced from the microbiology laboratory at King Salman Hospital in Ha’il, Saudi Arabia. Antibacterial efficacy was determined using minimum inhibitory concentration (MIC) assays, assessments of bacterial membrane damage, and qRT-PCR analysis of genes associated with antibiotic resistance. The findings revealed that the S. aureus strain exhibited resistance to multiple antibiotics with a resistance score of 0.44. RoME and BS demonstrated MICs of 0.125 mg/mL and 0.5 mg/mL, respectively. The assays indicated significant bacterial membrane damage and reduced expression of the norA, mdeA, and sel genes, which are implicated in resistance and virulence, respectively. The combination of BSs with plant extracts may provide innovative approaches for treating infections caused by multidrug-resistant bacteria, highlighting the potential of probiotic-derived BSs in combination with plant extracts. Full article

In the present work, we investigate the post-glacial aggradation of three tributary valleys draining the left hydrographic basin of the Tiber River in central Rome: the Murcia, Caffarella, and Grottaperfetta valleys. We describe the Upper Pleistocene–Holocene stratigraphic record of the alluvial successions occurring in the Caffarella Valley through the core data collected in a dedicatedly performed 35 m deep borehole. We provide seven ¹⁴C age constraints to the sediment aggradation which allow us to make a comparison with the Grottaperfetta and Murcia valleys, for which we present previously unpublished borehole data, and with the Tiber River Valley investigated in the previous literature. In particular, we highlight the effects of a mid-Holocene (5200–3800 yr BP) erosional phase, partially overlapping with the global 4.2 ka cooling/drying event, and we discuss the possible occurrence of a sea level fluctuation linked with this paleoclimatic event which has not been detected so far by other sedimentary records. Finally, we provide evidence for the widespread occurrence of a 6th century BCE (2550–2450 yr BP) overflooding phase that was previously observed only in the eastern portion of the Tiber River Valley in central Rome, which we suggest may be originated by concurrent intensive deforestation activity in central Italy. Full article

Within the ongoing scientific and disciplinary debate on the interplay between climate change and land-use governance, this paper highlights the critical role of urban planning and design in shaping environmental regeneration strategies for coastal urban areas vulnerable to flooding phenomena. These flood events—driven by the combined effects of sea-level rise (SLR) and riverine flood—represent one of the key challenges facing the “global risk society” given their increasing impact on urban areas and the tangible economic, social, and environmental damages they produce. In this context, this paper presents selected outcomes from the findings of the research project “Climate-proof planning and regeneration strategies for adaptation to sea-level rise. Experimentation and innovation in local urban planning in at-risk areas of the Lazio region”, conducted at Sapienza University of Rome. The project focuses on research and experimental planning in coastal areas of Lazio identified as being at risk of SLR-related flooding by 2100. It aims to define theoretical–methodological and operational references for urban regeneration with an ecosystemic approach within the framework of so-called climate-proof planning. This study examines three macro-areas, further subdivided into seven distinct sites, categorized by their prevailing urban functions. For each site, following a preliminary assessment of flood-prone zones, tailored design actions are proposed. These actions are framed within three overarching of urban resilience strategies, developed in previous research by the authors: “defence”, “adaptation”, and “relocation”. Full article

Background: Valvular heart disease remains a major global health issue, with mechanical prosthetic heart valves (MPHVs) widely used in surgical valve replacement. However, these devices carry a risk of thrombosis, particularly in the mitral position. Several mechanisms may be involved in this risk, but the role of oxidative stress (OxS) remains unclear. Our aim was to assess the relationship between OxS impairment and platelet activation. Methods: We analyzed data from a pilot, observational, monocentric study conducted at our anticoagulation clinic at Sapienza University of Rome, involving adult patients with MPHVs (aortic or mitral) on vitamin K antagonist therapy, enrolled between June and September 2024. Clinical data and blood samples were collected to evaluate markers of NOX2-mediated OxS (sNOX2-dp, H₂O₂) and platelet activation (sCD40L) using ELISA-based assays. Results: Our cohort included 30 patients with mitral MPHVs and 30 patients with aortic MPHVs (46.7% males, 53.3% females). Serum sNOX2-dp and H₂O₂ were significantly higher in patients with mitral MPHVs (28.69 [25.08–33.18] vs. 24.27 [17.30–26.41] pg/mL, p = 0.001, and 22.94 [15.79–27.33] vs. 16.73 [12.50–20.87] µM, p = 0.013, respectively) compared with aortic MPHV patients. sCD40L was significantly elevated in mitral versus aortic MPHVs (5.61 [3.69–6.89] vs. 3.65 [2.14–5.54] ng/mL, p = 0.009). Spearman’s correlation analysis showed a significant correlation between sNOX2-dp levels and sCD40L in both groups (mitral MPHVs: r_s = 0.521, p = 0.003; aortic MPHVs: r_s = 0.443, p = 0.014). Conclusions: Mitral MPHVs are associated with heightened OxS and platelet activation compared to aortic MPHVs. These findings may help explain the higher thrombotic risk observed with mitral valves and support differential management strategies. Full article

Addressing the planetary crisis associated with climate change, biodiversity loss, global pollution, and public health requires novel and holistic approaches. Here, we present the methodology and initial results of an experiment conducted in Rome within the framework of the National Biodiversity Future Center (NBFC) project, Spoke 6. The major objective of this study was to outline the planetary health approach as a lens to assess urban health. This transdisciplinary case study explored the relationship between urban traffic-related external exposome and pro-oxidative responses in humans and plants. This methodology is based on the integration of atmospheric dynamics modeling, state-of-the-art aerosol measurements, biomonitoring in human cohorts, in vitro cellular assays, and the assessment of functional trait markers in urban trees. The results indicate that short-term exposure to urban aerosols, even at low concentrations, triggers rapid oxidative and inflammatory responses in bronchial epithelial cells, modulates gene and miRNA expression, alters gut microbiota diversity, and induces functional trait changes in urban trees. This study also highlights the feedback mechanisms between vegetation and atmospheric conditions, emphasizing the role of urban greenery in modulating microclimate and exposure. The methodology and initial results presented here will be further analyzed in future studies to explore proof of a cause–effect relationship between short-term exposure to traffic-related environmental stressors in urban areas and oxidative stress in humans and plants, with implications for chronic responses. In a highly urbanized world, this evidence could be pivotal in motivating the widespread implementation of planetary health approaches for assessing urban health. Full article

Background: The increase in antimicrobial resistance (AMR) is a growing concern for global health. Understanding longitudinal trends in pathogen prevalence and resistance patterns is essential for guiding clinical management and antibiotic stewardship. This retrospective observational study analyzed respiratory microbial isolates collected from 2018 to 2023 in Tor Vergata University Hospital, Rome, Italy. Methods: The data were analyzed through WHOnet 2025 software, and the breakpoint references used are those of EUCAST 2025. The data analyzed included pathogen identification, antibiotic resistance rates, and specimen types. Time-trend analyses were conducted to assess changes in pathogen prevalence and antibiotic resistance rates over time, using the Pearson correlation coefficient and linear regression model. Results: More than 54,000 unique microorganism/drug associations were analyzed, with the majority of them relative to inpatients (over 90%). A. baumannii showed persistently high prevalence and drug resistance to multiple antibiotics. Significant upward resistance trends of K. pneumoniae to multiple antibiotics were observed. Approximately 20% of clinical isolates were fungi, also including some non-albicans Candida (NAC) species, which exhibit intrinsic resistance to azoles. Other microorganisms displayed variable trends in prevalence and resistance profiles. Conclusions: These findings underscore the dynamism of changing patterns of prevalence of microorganisms and their resistance to antimicrobials. They underscore the importance of continuous microbiological surveillance to optimize empirical therapies and guide infection control policies. Full article

Diarrhea-predominant irritable bowel syndrome (IBS-D) is a functional gastrointestinal disorder characterized by altered motility, abdominal pain, and dysbiosis—particularly reduced biodiversity and a lower abundance of butyrate-producing bacteria. Strategies that modulate the gut microbiota may offer therapeutic benefit. Clostridium butyricum (C. butyricum) CBM588 is a butyrate-producing probiotic with immunomodulatory properties and potential efficacy in treating gastrointestinal disorders. This pragmatic, prospective, open-label, single-arm interventional study assessed the clinical, microbial, and safety-related effects of an 8-week CBM588 supplementation, along with a low-fiber and low-residue diet, in 205 patients with IBS-D who attended Quisisana Nursing Home Hospital, Rome, Italy, between November 2024 and February 2025. The primary outcomes included the global symptom response, the Bristol Stool Scale (BSS), stool frequency, diarrhea episodes, abdominal pain (severity and frequency), bloating, bowel dissatisfaction, quality of life (QoL), safety, and treatment tolerability—measured using the IBS Symptom Severity Scale (IBS-SSS) and a standardized tolerability scale. CBM588, in patients treated with a low-fiber and low-residue diet, significantly improved all clinical endpoints, with a >80% reduction in diarrhea episodes; ~60% reductions in stool frequency and abdominal pain; and >50% improvements in bloating, bowel dissatisfaction, and QoL. Treatment was well tolerated (mean tolerability score 8.95 ± 0.88), with >95% adherence, and no serious adverse events were reported. The secondary outcomes included changes in gut microbiota. In a subset of patients, 16S rRNA gene sequencing showed increased α-diversity and enrichment of butyrate-producing genera (Agathobacter, Butyricicoccus, Coprococcus), which correlated with symptom improvement. Bloating increased in some patients, possibly related to fermentation activity. These findings support the C. butyricum CBM588 probiotic strain as a safe, well-tolerated, and microbiota-targeted intervention for IBS-D. Randomized controlled trials are warranted to confirm efficacy. Full article

Rome, historically regarded as a monumental center of Catholic Christendom, now stands as a multi-layered environment shaped by diverse religious communities whose overlapping architectures, rites, and narratives expand the city’s cultural memory. This article employs Warburg’s Mnemosyne methodology to investigate how symbolic motifs, architectural forms, and intangible practices—from Eastern Orthodox iconography to the Great Mosque of Rome’s transnational design—migrate, adapt, and reconfigure within Rome’s urban fabric. Drawing on interdisciplinary approaches from cultural memory studies, religious studies, and urban geography, it reveals how minority communities—Jewish, Muslim, Orthodox Christian, Protestant, Methodist, and Scientology—act as “memory agents”, negotiating visibility and introducing new heritage layers that challenge monolithic perceptions of Rome’s identity. The analysis underscores that intangible heritage, such as chanting, prayer, and interfaith festivals, is equally central to understanding how collective memory is produced and transmitted. Tensions arise when key stakeholders do not validate these emerging cultural forms or question their “authenticity”, reflecting the contested nature of heritage-making. Ultimately, Rome’s religious plurality, shaped by migration and historical transformations, emerges as a dynamic memoryscape. By recognizing the vital role of minority faiths in heritage-making, this study contributes to broader debates on cultural pluralism, super-diversity, and the evolving definitions of religious and cultural heritage in contemporary global cities. Full article

Promoting alternative modes of transportation such as cycling represents a valuable strategy to minimize environmental impacts, as confirmed in the main targets set out by the European Commission. In this regard, in cities throughout the world, there has been a significant increase in the construction of bicycle paths in recent years, requiring effective maintenance strategies to preserve their service levels. The continuous monitoring of road networks is required to ensure the timely scheduling of optimal maintenance activities. This involves regular inspections of the road surface, but there are currently no automated systems for monitoring cycle paths. In this study, an integrated monitoring and assessment system for cycle paths was developed exploiting Raspberry Pi technologies. In more detail, a low-cost Inertial Measurement Unit (IMU), a Global Positioning System (GPS) module, a magnetic Hall Effect sensor, a camera module, and an ultrasonic distance sensor were connected to a Raspberry Pi 4 Model B. The novel system was mounted on a e-bike as a test vehicle to monitor the road conditions of various sections of cycle paths in Rome, characterized by different pavement types and decay levels as detected using the whole-body vibration $a_{wz}$ index (ISO 2631 standard). Repeated testing confirmed the system’s reliability by assigning the same vibration comfort class in 74% of the cases and an adjacent one in 26%, with an average difference of 0.25 m/s², underscoring its stability and reproducibility. Data post-processing was also focused on integrating user comfort perception with image data, and it revealed anomaly detections represented by numerical acceleration spikes. Additionally, data positioning was successfully implemented. Finally, $a_{wz}$ measurements with GPS coordinates and images were incorporated into a Geographic Information System (GIS) to develop a database that supports the efficient and comprehensive management of surface conditions. The proposed system can be considered as a valuable tool to assess the pavement conditions of cycle paths in order to implement preventive maintenance strategies within budget constraints. Full article

Background/Objectives: Irritable bowel syndrome (IBS) is a complex disorder affecting 10% of the global population, but the underlying mechanisms remain poorly understood. By integrating multifluid metabolomics, we aimed to identify metabolite markers of IBS in a large population-based cohort. Methods: We included individuals from TwinsUK with and without IBS, ascertained using the Rome III criteria, and analysed serum (232 cases, 1707 controls), urine (185 cases, 1341 controls), and stool (186 cases, 1284 controls) metabolites (Metabolon Inc.). Results: After adjusting for covariates, and multiple testing, 44 unique metabolites (25 novel) were associated with IBS, including lipids, amino acids, and xenobiotics. Androsterone sulphate, a sulfated steroid hormone precursor, was associated with lower odds of IBS in both urine (0.69 [95% confidence interval = 0.56–0.85], p = 2.34 × 10⁻⁴) and serum (0.75 [0.63–0.90], p = 1.54 × 10⁻³. Moreover, suberate (C8-DC) was associated with higher odds of IBS in serum (1.36 [1.15–1.61]; p = 1.84 × 10⁻⁴) and lower odds of IBS in stool (0.76 [0.63–0.91]; p = 2.30 × 10⁻³). On the contrary, 32 metabolites appeared to be fluid-specific, including indole, 13-HODE + 9-HODE, pterin, bilirubin (E,Z or Z,Z), and urolithin. The remaining 10 metabolites were associated with IBS in one fluid with suggestive evidence (p < 0.05) in another fluid. Finally, we identified androgenic signalling, dicarboxylates, haemoglobin, and porphyrin metabolism to be significantly over-represented in individuals with IBS compared to controls. Conclusions: Our results highlight the utility of a multi-fluid approach in IBS research, revealing distinct metabolic signatures across biofluids. Full article

The Global Frameworks for Enhancing Natural and Cultural Capital, as expressed in the Charter of Rome (2014), have relevant importance in the broader context of sustainable development goals (SDGs). The intricate connection between cultural and natural heritage emerges in various documents from UNESCO, IUCN, and ICOMOS; however, the categories and methods for their assessment are not completely defined. Evolving from the concept of “mixed properties” used in the UNESCO definitions, we introduce the concept of CUNA places: cultural places of high relevance for nature. It provides a methodological tool for identifying and typifying these places based on their values, attributes, and significance. Then, we propose definitions and categories for three different CUNA typologies, comprising 16 categories, considering both tangible and intangible heritage. We also provide a preliminary application to the city of Rome, where a rich historical heritage intersects with outstanding natural values. All the typologies and categories of CUNA places are present in Rome, and further analysis could show the multifaced aspects represented here. On a broader scale, such an application offers the opportunity to enhance the heritages that could be valorized, strengthening the territorial identity, promoting sustainable development, and generating income for conservation efforts. Full article

Background/Objectives: The global spread of carbapenem-resistant Pseudomonas aeruginosa (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant Pseudomonas aeruginosa”) is to develop a globally applicable surveillance strategy. Methods: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. Conclusions: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA. Full article

Background: The recent resurgence of mpox in central Africa has been declared a new public health emergency of international concern (PHEIC) requiring coordinated international responses. Vaccination is a priority to expand protection and enhance control strategies, but the vaccine’s need exceeds the currently available doses. Intradermal (ID) administration of one-fifth of the standard modified vaccinia Ankara (MVA-BN) dose was temporarily authorized during the 2022 PHEIC. Studies conducted before 2022 provided evidence about the humoral response against the vaccinia virus (VACV) after vaccination but not against the mpox virus (MPXV). Moreover, no data are available on the T-cell response elicited by MVA-BN administered subcutaneously or intradermally. Methods: We compare the two vaccine administration routes according to reactogenicity (n = 943) and immunogenicity (n = 225) of vaccine recipients attending INMI Spallanzani hospital during the 2022 vaccination campaign in Rome, Italy. Results: We found that the ID route elicited higher titers of MPXV-specific IgG (mean difference of 0.26 log₂, p = 0.05) and nAbs (0.24 log₂, p = 0.08) than the subcutaneous (SC) route one month after the complete vaccination cycle. At the same time, no evidence for a difference in cellular response was found. Conclusions: MVA-BN was globally well tolerated despite higher reactogenicity for the ID than the SC route, especially for the reactions at the local injection site. The ID dose-sparing strategy was proven safe and immunogenic and would make vaccination available to more people. Our data support the current WHO recommendation of using the ID route in low–medium-income countries (LMIC), although response data in people infected with the new 1b clade are urgently needed. Full article

Global environmental change is rapidly altering the dynamics of terrestrial vegetation, with consequences for the functioning of the Earth system. Recent studies show that climate change is influencing the phenology and distribution of plants. Airborne pollen reflects the flowering period of the plant, which is influenced by meteorological variables such as temperature and rainfall. The analysis of pollen trends is a very useful tool for understanding the effects of climate change on vegetation. In fact, it is accepted that the onset and peak abundance of certain pollen types should be used as possible bioindicators of climate change. The aim of the work is to analyze the presence of various pollen in Rome—from their release from the anthers to their permanence in the atmosphere, the trends of phenological (start, length, and end of the pollen season) and production (pollen abundance and pollen peaks) pollen indicators, the trends of the meteorological variables mainly involved (temperature and precipitation), and any relationships between pollen and meteorological variables, also based on the variation in vegetation. In the period considered, the analysis of the pollen spectra shows an increasing trend in herbaceous taxa, probably attributed to a gradual abandonment of farming practices in the neighboring area, which in recent years has been the subject of intense new construction activity and to a progressive deterioration in the maintenance of green areas. Full article