Search Results (196)

The safe and efficient integration of small unmanned aircraft systems (sUAS) into the National Airspace System (NAS) requires a systems-based understanding of the interrelations among human, technological, and regulatory components. Existing Federal Aviation Administration (FAA) guidelines restrict most operations to visual line of sight (VLOS), which constrains operational scalability and underscores the need for system-level innovations supporting beyond-visual-line-of-sight (BVLOS) operations. This study adopted a socio-technical systems approach to evaluate how first-person view (FPV) technologies influence operator workload and situational awareness (SA), key human performance elements within the broader sUAS safety system. Participants meeting FAA Part 107 eligibility criteria were assigned to one of three visual configurations: (a) traditional VLOS, (b) FPV using a 21-inch monitor, or (c) FPV with immersive goggles. Workload was measured with the NASA Task Load Index (NASA-TLX), and Level 1 SA was assessed via post-task recall. ANOVA results revealed no statistically significant differences across visual conditions, indicating no evidence that FPV integration either increased cognitive load or impaired perceptual awareness compared to traditional methods. Complementary analysis of NASA’s Aviation Safety Reporting System (ASRS) identified SA as the most recurrent human-factor issue, suggesting system-level implications for human–machine interaction and training design. These findings contribute to the systemic understanding of human factors in UAS operations, supporting FPV’s potential as a viable subsystem for achieving safe and effective BVLOS integration within complex socio-technical aviation systems. Full article

Protoparvoviruses are highly contagious pathogens that cause severe, often fatal diseases in both domestic and wild carnivores. Golden jackal (Canis aureus) populations have experienced expansion in recent years, increasingly occupying urban and peri-urban areas. Despite this, they remain largely overlooked in scientific research. This study aimed to detect and characterise Protoparvovirus carnivoran1 circulating in a golden jackal population in Croatia and to assess their role in the epidemiology of parvovirus infections in companion animals. Small intestines from 55 jackals hunted in 2024 and 2025 were tested for Protoparvovirus carnivoran1 using real-time PCR. Positive samples were found across all sampling sites, with an overall positivity rate of 40%. Based on characteristic amino acid residues within the VP2 protein, the viruses detected in jackals were classified as feline panleukopenia virus (FPV). Phylogenetic analysis of the VP2 protein demonstrated considerable genetic diversity among strains circulating in Croatia. Additionally, a distinct group was identified, shared exclusively by Croatian domestic cats and golden jackals. Amino acid analysis revealed the novel A91T mutation, found only in jackals, and the E411Q mutation, unique to Croatian FPV strains. Structural modelling of the VP2 protein indicates that the observed mutations are located on the protein surface, within the antibody-binding site. These findings highlight the potential role of wild carnivores in parvovirus epidemiology and underscore the importance of including them in future surveillance and research efforts. Full article

Background: Feline panleukopenia virus (FPV) causes acute and frequently fatal disease in cats, underscoring the urgent need for safe, rapidly effective, and scalable vaccines. While virus-like particle (VLP) vaccines are inherently safe and immunogenic, their development is constrained by low yields of recombinant protein in insect cell expression systems. Methods: An optimized baculovirus expression vector system (BEVS) incorporating the hr1-p6.9-p10 transcriptional enhancer and the Ac-ie-01 anti-apoptotic gene was employed to enhance recombinant protein production. VP2 expression levels, viral titers, and hemagglutination activity were quantified using qPCR, SDS-PAGE/Western blotting, transmission electron microscopy (TEM), and functional assays. Immunogenicity and protective efficacy were assessed in both mice and cats through serological analysis, neutralizing antibody detection, and post-challenge clinical monitoring. Results: The optimized BEVS enhanced recombinant protein transcription by 1.5-fold, viral titers by 3.7-fold, and hemagglutination activity by 15-fold. The purified protein self-assembled into uniform 25 nm virus-like particles (VLPs). Immunization elicited earlier responses compared to commercial vaccines. Vaccinated cats maintained normal body temperature, stable leukocyte counts, and minimal viral shedding following FPV challenge. Conclusions: This study validates an enhanced BEVS that effectively overcomes VP2 yield constraints and generates highly immunogenic FPV VLPs. The platform enables rapid-onset protection and offers a scalable strategy for next-generation FPV vaccine development. Full article

Offshore floating photovoltaic (FPV) platforms are usually deployed in shallow waters with large tidal variations, where the modules of FPV are connected with each other via the connectors to form an array and mounted to the seabed via the mooring system. Therefore, the mooring system and module connectors have significant influence on the dynamic response characteristics of FPV. In targeting such shallow waters with large tidal ranges, this paper proposes four integrated mooring-connection schemes based on configuration and parameter customization guided by adaptability optimization, including two kinds of mooring systems, named as horizontal mooring system and catenary mooring system with clumps, and two kinds of connection schemes, named as cross-cable connection and hybrid connection, are proposed. The feasibility of the mooring systems to adhere to the tidal range and the influence of the connection schemes on the dynamic response of the FPV are numerically investigated in detail. Results indicate the two mooring systems have comparable positioning performance; horizontal mooring offers slightly better tidal adaptability but much higher mooring tension, compromising system safety. Hybrid connection yields smaller surge amplitudes than cross-cable connection but generates excessively large connection forces, also posing safety risks. Comprehensive comparison indicates that catenary mooring with clumps combined with cross-cable connection imposes lower requirements on platform structural safety factors, while horizontal mooring with cross-cable connection exhibits stronger adaptability to water level and environmental load direction changes in shallow waters. Full article

Droplet microfluidics enables high-throughput, compartmentalized reactions using minimal reagent volumes, but most implementations rely on precision-fabricated chips and external pumping systems that limit portability and accessibility. Here, we present a handheld vibrational droplet generator that repurposes a consumer electric toothbrush and a modified disposable pipette tip to produce nearly monodisperse water-in-oil droplets without microfluidic channels or syringe pumps. The device is powered by the toothbrush’s built-in motor and controlled by a simple 3D-printed adapter and adjustable counterweight that tune the vibration amplitude transmitted to the pipette tip. By varying the aperture of the pipette tip, droplets with diameters from ~100–300 µm were generated at rates of ~100 droplets s⁻¹. Image analysis revealed narrow size distributions with coefficients of variation below 5% in typical operating conditions. We further demonstrate proof-of-concept applications in digital loop-mediated isothermal amplification (LAMP) and microbiological colony-forming unit (CFU) assays. A commercial feline parvovirus (FPV) kit manufactured by Beyotime Biotechnology Co., Ltd. (Shanghai, China), three template concentrations yielded emulsified reaction droplets that remained stable at 65 °C for 45 min and produced distinct fractions of fluorescent-positive droplets, allowing estimation of template concentration via a Poisson model. In a second set of experiments, the device was used as a droplet-based spreader to dispense diluted Escherichia coli suspensions onto LB agar plates, achieving uniform colony distributions across the plate at different dilution factors. The proposed handheld vibrational generator is inexpensive, easy to assemble from off-the-shelf components, and minimizes dead volume and cross-contamination because only the pipette tip contacts the sample. Although the current prototype still exhibits device-to-device variability and moving droplets in open containers complicate real-time imaging, these results indicate that toothbrush-based vibrational actuation can provide a practical and scalable route toward “lab-in-hand” droplet assays in resource-limited or educational settings. Full article

Floating photovoltaic (FPV) systems are increasingly deployed in offshore environments. Among various FPV concepts, membrane-type platforms offer distinct advantages, including reduced weight, lower material consumption, and cost-effectiveness. This study investigates the hydrodynamic response of a membrane-type offshore FPV system through a 1:40 scale physical model test based on the Ocean Sun prototype. Static-water free-decay tests were first conducted to determine the natural periods and damping characteristics in heave, surge, and pitch motions. Subsequently, irregular-wave tests were performed under seven sea states representative of an offshore demonstration site. Free-decay results show model-scale natural periods of approximately 1.0 s for heave, 0.8 s for pitch, and 15 s for surge. The long surge natural period avoids resonance with short-period waves, while the high damping in heave and pitch effectively limit dynamic amplification. Under irregular waves, heave and pitch motions remain small, whereas surge motion exhibits pronounced long-frequency excursions. Spectral analysis reveals a dominant low-frequency surge peak at f ≈ 0.067 Hz (corresponding to the natural period of 15 s), superimposed with higher-frequency components associated with wave-induced motions. A strong correlation is observed between low-frequency surge and mooring tensions. Across Sea States 1–6, the motion responses increase gradually, while a marked rise in the exceedance probability of mooring forces occurs only in the most severe sea state. Weibull extreme-value fits show good linearity, indicating that the measured extremes are statistically consistent. The results provide experimental data and design insights for membrane-type FPV systems, establishing a foundation for future hydroelastic studies. Full article

This study investigated the prevalence of 11 common pathogens in dogs and cats in Shenzhen, China, from January 2022 to March 2024, aiming to enhance the understanding of their epidemiological characteristics for improved disease control strategies. Diagnostic testing for the target pathogens was performed using polymerase chain reaction (PCR), colloidal gold test strips, or fluorescence immunoassay. Statistical analysis revealed that among 13,134 cats, Feline Panleukopenia Virus (FPV) showed the highest prevalence (35.83%), followed by Feline Calicivirus (FCV, 26.20%), Feline Infectious Peritonitis Virus (FIPV, 22.00%), and Feline Herpesvirus (FHV, 15.76%). Among 3626 dogs, Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) were predominant, showing a prevalence of 54.55% and 42.83%, respectively. Risk factor analysis showed that most infections occurred in unvaccinated animals and young individuals (<1 year old), with higher incidences in winter and spring. Logistic regression indicated that sex, age, and season were significantly associated with FPV, FHV, and FIPV infections, while age and season were associated with FCV, CPV, and CDV infections (sex showed no association). This study contributes to the epidemiological knowledge of common infectious diseases in dogs and cats, providing a theoretical basis for disease prevention in dogs and cats. Full article

African horse sickness (AHS) is a lethal vector-borne disease caused by African horse sickness virus (AHSV) and represents a major threat to equine health and the horse industry. In 2020, outbreaks of AHS caused by AHSV serotype 1 (AHSV-1) were reported in Thailand, increasing the risk of AHS introduction into China. Given the safety issues associated with currently available live attenuated AHS vaccines, the development of safer and more effective vaccination strategies is urgently needed. In this study, we constructed a recombinant fowlpox virus (rFPV) expressing the AHSV-1 VP2 protein as a candidate vaccine, designated rFPV-VP2. The recombinant virus was verified by PCR and Western blot analysis, which confirmed the successful expression of VP2. Preliminary immunization trials were conducted in both mice and horses, and immune responses were evaluated via an indirect enzyme-linked immunosorbent assay (iELISA) and immunofluorescence assay (IFA). The results revealed that VP2-specific antibodies were successfully induced in the serum of rFPV-VP2-immunized animals. Notably, serum from immunized horses showed specific reactivity with AHSV-1, confirming the induction of AHSV-1-specific immune responses. Therefore, these results demonstrate that rFPV-VP2 is a promising candidate vaccine for AHSV-1 and provide a scientific basis for the development of safer preventive strategies. Full article

This study investigates the optimization of Pumped Storage Hydropower (PSH) integrated with Floating Photovoltaic (FPV) systems, with a focus on sustainable energy management. A nonlinear programming framework combined with scenario analysis was applied to a real hydropower system in Trentino, Italy. The optimization maximizes revenues through energy arbitrage while accounting for water resource and environmental objectives. Upgrading the traditional hydropower plant to PSH operation increases revenues by 4–8% over two hydrological years. Multi-objective optimization further reveals large gains in water availability, confirming PSH’s dual role as energy storage and water management infrastructure. Different FPV configurations analyzed show a 2–3% increase in photovoltaic energy yield due to the water-cooling effect, while the overall hybrid PSH–FPV integration mainly reduces grid dependency and pumping-related emissions, with near-complete decarbonization achievable under optimized scheduling. Overall, PSH provides the primary economic and operational advantage, while FPV strengthens sustainability, enabling resilient hydro–solar operation and contributing to renewable integration and decarbonization in future energy systems. Full article

Floating photovoltaic systems represent a promising solution for renewable energy generation, offering an alternative to agricultural land consumption. However, these installations have the potential to exert an effect on the aquatic ecosystem, emphasizing the necessity of effective monitoring strategies also related to system management issues. In this paper, the use of an unmanned surface vehicle, which can also operate as an autonomous surface vehicle, is proposed to overcome many difficulties of maintenance and monitoring in aquatic environments. A review of the extant literature reveals the scarcity of a cohesive monitoring framework for these plants, highlighting the urgent need for standardized guidelines for plant management and water quality monitoring. The implementation of automated plants directly addresses this gap by providing a tool for efficient and sustainable monitoring tasks, enabling, at the same time, aquatic ecosystem protection and energy production optimization. To address these challenges, a low-cost prototype of an autonomous surface vehicle is proposed. Preliminary test results on trajectory control and obstacle recognition are reported. Full article

Feline herpesvirus type-1 (FHV-1), a double-stranded DNA virus, which is a highly infectious upper respiratory tract infection of felids, particularly in kittens. Droplet digital PCR (ddPCR) provides an absolute quantification method with high sensitivity and accuracy. This study aimed to develop a highly sensitive and accurate ddPCR assay for the detection of FHV-1. We designed primers and a probe targeting the FHV-1 glycoprotein D (gD) gene and evaluated the assay’s limit of detection (LOD), sensitivity, repeatability, and specificity in comparison to quantitative real-time PCR (qPCR). The developed ddPCR assay demonstrated a strong linear dynamic range (R² ≥ 0.99) and an exceptionally low LOD of 0.18 copies/μL, which was significantly more sensitive than the method qPCR (LOD ~10 copies/μL). Additionally, the assay exhibited high specificity with no cross-reactivity against other common feline pathogens (feline calicivirus, FCV; feline panleukopenia virus, FPV; feline infectious peritonitis virus, FIPV; Bordetella bronchiseptica and Chlamydia felis) and displayed outstanding repeatability (inter-run CV < 1.35). When applied to 118 clinical samples, the ddPCR assay achieved a significantly higher positive detection rate (27.4%) compared to qPCR (14.8%). In conclusion, we have successfully established a reliable ddPCR assay for the absolute quantification of FHV-1, providing a superior tool for laboratory diagnosis and research. Full article

This work presents the structural analysis and validation of a sub-250 g FPV drone chassis, emphasizing both theoretical rigor and practical applicability. The novelty of this contribution lies in four complementary aspects. First, the structural philosophy introduces a screwless frame with interchangeable arms, joined through interlocking mechanisms inspired by traditional Japanese joinery. This approach mitigates stress concentrations, reduces weight by eliminating fasteners, and enables rapid arm replacement in the field. Second, validation relies on nonlinear static and transient FEM simulations, explicitly including crash scenarios at 5 m/s, systematically cross-checked with bench tests and instrumented flight trials. Third, unlike most structural studies, the framework integrates firmware (Betaflight), GPS, telemetry, and real flight performance, linking structural reliability with operational robustness. Finally, a practical materials pathway was implemented through a dual-track strategy: PETG for rapid, low-cost prototyping, and carbon fiber composites as the benchmark for production-level performance. Nonlinear transient FEM analyses were carried out using Inventor Nastran under multiple load cases, including maximum motor acceleration, pitch maneuvers, and lateral impact at 40 km/h, and were validated against simplified analytical models. Experimental validation included bench and in-flight trials with integrated telemetry and autonomous features such as Return-to-Home, demonstrating functional robustness. The results show that the prototype flies correctly and that the chassis withstands the loads experienced during flight, including accelerations up to 4.2 G (41.19 m/${\mathrm{s}}^2$), abrupt changes in direction, and high-speed maneuvers reaching approximately 116 km/h. Quantitatively, safety factors of approximately 5.3 under maximum thrust and 1.35 during impact confirm sufficient structural integrity for operational conditions. In comparison with prior works reviewed in this study, the key contribution of this work lies in unifying advanced, crash-resilient FEM simulations with firmware-linked flight validation and a scalable material strategy, establishing a distinctive and comprehensive workflow for the development of sub-250 g UAVs. Full article

Floating and offshore photovoltaic (FPV) installations present a promising solution for addressing land-use conflicts while enhancing renewable energy production. With an estimated global offshore PV potential of 4000 GW, FPV systems offer unique advantages, such as increased efficiency due to water cooling effects and synergy with other offshore technologies. However, challenges related to installation costs, durability, environmental impacts, and regulatory gaps remain. This review provides a comprehensive and critical analysis of FPV advancements, focusing on inland, nearshore, and offshore applications. A systematic evaluation of recent studies is conducted to assess technological innovations, including material improvements, mooring strategies, and integration with hybrid energy systems. Furthermore, the economic feasibility of FPVs is analysed, highlighting cost–benefit trade-offs, financing strategies, and policy frameworks necessary for large-scale deployment. Environmental concerns, such as biofouling, wave-induced stress, and impacts on aquatic ecosystems, are also examined. The findings indicate that while FPV technology has demonstrated significant potential in enhancing solar energy yield and water conservation, its scalability is hindered by high capital costs and the absence of standardised regulations. Future research should focus on developing robust offshore floating photovoltaic (OFPV) designs, optimising material durability, and establishing regulatory guidelines to facilitate widespread adoption. By addressing these challenges, FPVs can play a critical role in achieving global climate goals and accelerating the transition to sustainable energy systems. Full article

This paper investigates the integration of hydropower and solar energy within the Lower Olt River cascade as a pathway toward sustainable energy generation in Romania. The study focuses on the conceptual design of future hybrid power plants consisting of existing hydropower facilities where floating photovoltaic panels are proposed to be installed on the reservoir’s surfaces. An estimation of electricity production from both sources was performed, followed by the formulation of a trading strategy for the July–September 2025 period. The paper also explores the interaction between tactical and strategic management in hydropower operation and planning, describing how forecasting and decision-making processes are structured within the institutional framework. Finally, results for the selected hydropower plants demonstrate the positive influence of floating photovoltaic deployment on company performance, the national energy mix, and the overall sustainability of energy generation in Romania. Full article

Stray cats (Felis vaga) are key hosts for feline and zoonotic pathogens. From June to August 2024, we conducted a cross-sectional study across six districts in Shenzhen, China, involving 126 cats sampled from three types of sites. Multiple specimens were tested via quantitative real-time PCR (qPCR) for feline coronavirus type I (FCoV-I), feline calicivirus (FCV), feline herpesvirus type I (FHV-I), feline panleukopenia virus (FPV), and rabies virus (RABV); serum was analyzed for RABV-neutralizing antibodies by the fluorescent antibody virus neutralization (FAVN) assay. The overall pathogen positivity was 89.68%. FPV was most prevalent (61.90%), followed by FCV (57.14%), FCoV-I (46.83%), and FHV-I (23.02%). No RABV nucleic acid was detected. The co-infection rate reached 62.70%, primarily dual infections (33.33%). Geographical variation was observed, with significantly higher FCoV-I in Longgang than Futian (p < 0.05). RABV seropositivity was only 6.00%. FCV and FPV co-occurred most frequently (Jaccard = 0.456). All pathogen pairs had relative risk (RR) > 1, suggesting non-random co-infections, though not significant after correction. In summary, major feline pathogens are widespread with frequent co-infections among Shenzhen stray cats, while low rabies immunity indicates potential public health risk. Targeted control measures are warranted. Full article