Search Results (67)

Trematode infections caused by Fasciolidae and Paramphistomidae remain widespread in livestock, resulting in substantial economic losses. The two distinct fluke families are difficult to distinguish morphologically, and molecular identification provides the most reliable means of accurate diagnosis. In Ecuador, however, molecular data on these parasites are scarce. In this study, we collected trematodes from cattle rumen and bile ducts, molecularly identified them, and assessed their phylogenetic relationship to Fasciola hepatica to determine their introduction pathways into South America. Genomic DNA was extracted, and PCR was used to amplify the ITS2 (~500 bp) and COXI (~266 bp) regions; all amplicons were Sanger-sequenced. Phylogenetic trees for both markers were constructed using a Maximum Likelihood approach with 1000 bootstrap replicates in CIPRES v3.3. The rumen fluke exhibited 99% ITS2 and COXI similarity to an Indian Cotylophoron cotylophorum strain, while the bile-duct fluke showed 99% ITS2 and 100% COXI similarity to F. hepatica isolates from Australia and Nigeria, respectively. Distinct single-nucleotide polymorphisms (SNPs) in the ITS2 chromatograms suggest a diploid genome structure in both trematode species. This is the first report of C. cotylophorum in Ecuador, and its presence may be linked to the late 19th-century introduction of Zebu cattle (Bos taurus indicus) from India. Full article

According to research, intrauterine exposure to non-pathogenic maternal microorganisms during pregnancy is influenced by the mother’s nutritional, metabolic, and immunological status. This study investigates the association between maternal gut microbiota composition, fetal development, and neonatal microbiota, with the aim of exploring their interconnected health dynamics. A cohort-based correlational study was conducted involving 114 women (≥18 years old, ≤12 weeks of gestation) attending prenatal consultations at the ISSSTE General Hospital in Pachuca de Soto, Hidalgo, México. Data were collected at four stages: before 11 weeks, at 11–14 weeks, at 20–24 weeks, and at 31 weeks of pregnancy. Assessments included anthropometric measurements, biochemical markers, and intestinal microbiota analysis. The Firmicutes/Bacteroidetes (F/B) ratio positively correlated with venous duct flow and expected weight for gestational week (r = 0.02272, p = 0.0323; r = 0.2344, p = 0.0271). Bacteroidetes showed a positive correlation with birth weight (r = 0.2876, p = 0.0063), birth height (r = 0.5889, p < 0.001), and head circumference (r = 0.2163, p = 0.0418). Correlation analysis revealed significant relationships between maternal and neonatal microbiota, particularly for Bacteroidetes and Firmicutes. The findings suggest that maternal gut microbiota significantly influences fetal growth and neonatal microbiota composition. These insights underscore the importance of maternal health during pregnancy. Full article

The current examination used a multi-criteria decision-making (MCDM) approach to optimize the roughness parameters of S-shaped ribs (SSRs) in a solar thermal collector (STC) duct using air as the working fluid. Different SSRs were tested to identify the combination of parameters resulting in the best performance. Geometrical parameters such as relative roughness pitch ($P_R/e_{RH}$) varied from 4 to 12, relative roughness height ($e_{RH}/D_{hd}$) from 0.022 to 0.054, arc angle (${\alpha }_{Arc}$) from 30° to 75°, and relative roughness width ($W_{Duct}/w_{RS}$) from 1 to 4. The Nusselt number (${Nu}_{RP}$) and friction factor ($f_{RP}$), findings which impact the STC performance, rely on SSRs. The performance measurements show that no combination of SSR parameters lead to the best enhancement heat transfer rate at low enhancement in the friction. So, a hybrid multi-criteria decision-making strategy using the Analytical Hierarchy Process (AHP) for criterion significance and Multi Attributive Border Approximation Area Comparison (MABAC) for alternative ranking was used to determine which combination of geometrical parameters will result in the optimum performance of a roughened STC. This work employs a hybrid MCDM technique to optimise the effectiveness of an STC roughened with SSRs. To optimize the SSR design parameters, this study used the hybrid AHP-MABAC technique for analytical assessment of a roughened STC. The optimization results showed that the STC roughened with SSRs achieved the optimum performance at $P_R/e_{RH}$ = 8, $e_{RH}/D_{hd}$ = 0.043, ${\alpha }_{Arc}$ = 60° and $W_{Duct}/w_{RS}$ = 3. Full article

Extracellular vesicles (EVs) contain bioactive lipids that play a key role in pathophysiology. We hypothesized that EVs released from salt-loaded hypertensive diabetic db/db mice have increased bioactive lipid content that inhibits intracellular calcium mobilization and increases the activity of renal epithelial sodium channels (ENaC). An enrichment of sphingomyelins (SMs) was found in small urinary EVs (uEVs) isolated from salt-loaded hypertensive diabetic db/db mice (n = 4) compared to non-salt loaded db/db mice with diabetes alone (n = 4). Both groups of mice were included in the same cohort to control for variability. Cultured mouse cortical collecting duct (mpkCCD) cells loaded with a calcium reporter dye and challenged with small uEVs from hypertensive diabetic db/db mice showed a decrease in calcium mobilization when compared to cells treated with small uEVs from diabetic db/db mice. The amiloride-sensitive transepithelial current was increased in mpkCCD cells treated with small uEVs with abundant sphingomyelin content from hypertensive diabetic db/db mice in a dose- and time-dependent manner. Similar results were observed in mpkCCD cells and Xenopus 2F3 cells treated with exogenous sphingomyelin in a time-dependent manner. Single-channel patch clamp studies showed a decrease in ENaC activity in cells transiently transfected with sphingomyelin synthase 1/2 specific siRNA compared to non-targeting siRNA. These data suggest EVs with high sphingomyelin content positively regulate renal ENaC activity in a mechanism involving an inhibition of calcium mobilization. Full article

Background/Objectives: Golgi protein 73 (GP73) is a transmembrane protein expressed by epithelial cells of the bile duct in the normal liver. High serum levels of GP73 have been detected in patients with acute or chronic liver diseases, MASLD, and its measurement has been suggested as a potential biomarker for liver fibrosis staging. We evaluated the utility of GP73 in the diagnosis of MASLD, MASH, and for liver fibrosis staging. Methods: We performed a literature scoping review to map the current evidence about the accuracy of GP73 in patients with MASLD. We searched in Medline and EMBASE for English studies reporting an AUC value of GP73 in diagnosing MASLD and MASH and evaluating GP73 for fibrosis staging. A narrative synthesis of the evidence was conducted. Moreover, we performed an observational study including 84 patients with MASLD, of which 60 were biopsy-confirmed MASH, and different liver fibrosis stages, and 15 healthy controls. Serum GP73 levels were determined using a chemiluminescent assay and reported as mean and standard deviation (SD). Sensitivity (SE), specificity (SP), the area under the receiver operating characteristic (AUROC) curve, and the optimal cut-off value were calculated. Data were considered statistically significant when p < 0.05. Results: Available studies evaluating GP73 in MASLD reported the ability to discriminate MASH from simple steatosis and distinguish patients at different fibrotic stages, but the evidence is still scarce. Our experimental study showed that the serum levels of GP73 were 30 ± 12 ng/mL in MASLD and 32 ± 12 ng/mL in MASH patients and were statistically higher than those of the control group (19 ± 30 ng/mL), increasing from liver fibrosis stage F0 to F4. GP73 levels were significantly higher in patients with significant and advanced fibrosis than controls and no significant fibrosis (p > 0.05). ROC analysis demonstrated that serum GP73 had a good diagnostic potential for MASLD (AUROC 0.85; SE 90%; SP 73%), MASH (AUROC 0.75; SE 82%; SP64%), and significant fibrosis (AUROC 0.7; SE 56%; SP 79%) and was better than other biomarkers for chronic liver diseases. Conclusions: Serum GP73 could support clinicians in the evaluation of patients with MASH and significant fibrosis. Full article

The three-dimensional (3D) reconstruction of the hepatic duct tree is significant for the minimally invasive surgery of hepatobiliary stone disease, which can be influenced by the quantity of the CT scans of hepatic ducts. If insufficient CT scans with low inter-slice resolution are directly utilized for 3D reconstruction, discontinuities and gaps will emerge in the reconstructed hepatic duct tree. In this paper, a novel end-to-end deep learning framework is designed for the inter-slice super-resolution segmentation of the CT slices of hepatic ducts, which can improve the 3D reconstruction performance in the inter-slice dimension. Specifically, the framework cascades into an inter-slice super-resolution subnetwork and a segmentation subnetwork. A deep learning network is introduced as the inter-slice super-resolution subnetwork to generate an intermediate slice between two adjacent CT slices in the simulated CT scans with low inter-slice resolution. To capture the spatiotemporal correlation existing in the CT scans of hepatic ducts, the ConvLSTM is introduced into the U-Net-like segmentation subnetwork in the high-dimensional feature space. To further suppress the problems of discontinuities and gaps, a structure-aware loss function is proposed by incorporating the structural similarity index measure (SSIM) as a regulator to dynamically assign the contribution of the generated CT slice to the total loss of the designed framework. Experimental results demonstrate that our proposed framework performs better segmentations for hepatic ducts than several existing deep learning networks with the performance of 0.7690 DICE and a 0.7712 F1-score, which is beneficial for the 3D reconstruction of the hepatic duct tree. Full article

This study presents a numerical evaluation of a Horizontal Rectilinear Earth–air Heat Exchanger (EAHE), considering the climatic and soil conditions of Viamão, Brazil, a subtropical region. The Constructal Design method, combined with the Exhaustive Search, was utilized to define the system constraints, degree of freedom, and performance indicators. The degree of freedom was characterized by the aspect ratio between the vertical and horizontal lengths of the elliptical cross-section duct (H/L). The performance indicators for the EAHE configurations were assessed based on thermal potential (TP) and pressure drop (PD). The Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) was applied for multi-objective evaluation, and a methodology for EAHE is proposed. The problem was solved using FLUENT software (version 2024 R2), which employs the Finite Volume Method to solve the conservation equations for mass, momentum, and energy. The (H/L)_T,o = 6.0 configuration showed a 16.4% increase in thermal performance for heating and 15.9% for cooling compared to the conventional circular duct. Conversely, the (H/L)_F,o = 1.0 configuration reduced pressure loss by 65.33%. The integration of Constructal Design with TOPSIS facilitated the identification of optimized geometries that achieve a balance between performance indicators and those that specifically prioritize thermal or fluid dynamic aspects, being this approach an original scientific contribution of the present work. Full article

Fasciola hepatica is a liver fluke that resides in the bile ducts of various mammals. The parasitosis leads to economic losses in animal production estimated at USD 3.2 billion annually. It is also considered a zoonosis of great significance and a problem for public health affecting 2.4 million people worldwide. Nevertheless, besides the negative aspects of infestation, the antigens released by the fluke, F. hepatica Excretory-Secretory Products (Fh-ES) contain several immunomodulatory molecules that may be beneficial during the course of type I diabetes, multiple sclerosis, ulcerative colitis, or septic shock. This phenomenon is based on the natural abilities of adult F. hepatica to suppress proinflammatory responses. To underline the molecular basis of these mechanisms and determine the role of microRNA (miRNA) in the process, lipopolysaccharide (LPS)-activated THP-1 macrophages were stimulated with Fh-ES, followed by miRNA microarray analyses. Surprisingly, no results indicating changes in the miRNA expression profile were noted (p < 0.05). We discuss potential reasons for these results, which may be due to insufficient sensitivity to detect slight changes in miRNA expression or the possibility that these changes are not regulated by miRNA. Despite the negative data, this work may contribute to the future planning of experiments by other researchers. Full article

The primary hepatic leiomyosarcoma is a rare malignant tumor arising from the smooth muscle cells in the hepatic vessels, bile ducts, and ligamentum teres. It is considered a subtype of hepatic sarcomas. We report awkward ¹⁸F-FDG PET-CT findings of a primary hepatic leiomyosarcoma masquerading as a benign hepatic tumor, which were confirmed by histopathological and immunohistochemical examinations in a 78-year-old woman. Full article

Fasciolosis, a globally re-emerging zoonotic disease, is mostly caused by the parasitic infection with Fasciola hepatica, often known as the liver fluke. This disease has a considerable impact on livestock productivity. This study aimed to evaluate the fluke burdens and faecal egg counts in goats that were administered phage clones of cathepsin L mimotopes and then infected with F. hepatica metacercariae. Additionally, the impact of vaccination on the histology of the reproductive system, specifically related to egg generation in adult parasites, was examined. A total of twenty-four goats, which were raised in sheds, were divided into four groups consisting of six animals each. These groups were randomly assigned. The goats were then subjected to two rounds of vaccination. Each vaccination involved the administration of 1 × 10¹³ phage particles containing specific mimotopes for cathepsin L2 (group 1: PPIRNGK), cathepsin L1 (group 2: DPWWLKQ), and cathepsin L1 (group 3: SGTFLFS). The immunisations were carried out on weeks 0 and 4, and the Quil A adjuvant was used in combination with the mimotopes. The control group was administered phosphate-buffered saline (PBS) (group 4). At week 6, all groups were orally infected with 200 metacercariae of F. hepatica. At week 22 following the initial immunisation, the subjects were euthanised, and adult F. hepatica specimens were retrieved from the bile ducts and liver tissue, and subsequently quantified. The specimens underwent whole-mount histology for the examination of the reproductive system, including the testis, ovary, vitellaria, Mehlis’ gland, and uterus. The mean fluke burdens following the challenge were seen to decrease by 50.4%, 62.2%, and 75.3% (p < 0.05) in goats that received vaccinations containing cathepsin L2 PPIRNGK, cathepsin L1 DPWWLKQ, and cathepsin L1 SGTFLFS, respectively. Animals that received vaccination exhibited a significant reduction in the production of parasite eggs. The levels of IgG1 and IgG2 isotypes in vaccinated goats were significantly higher than in the control group, indicating that protection is associated with the induction of a mixed Th1/Th2 immune response. The administration of cathepsin L to goats exhibits a modest level of efficacy in inducing histological impairment in the reproductive organs of liver flukes, resulting in a reduction in egg output. Full article

Background and Objectives: Thyroid ectopy represents a rare disease with an incidence of 0.3–1/100,000. It occurs due to the defective embryological process of the thyroid gland development. The thyroid ectopic tissue may suffer malignant transformation. This review aims to shed light on the roles that I-131 SPECT/CT (radioiodine 131 single-photon emission tomography fused with computed tomography) and F-18 PET/CT (fluorodeoxyglucose F18 positron emission tomography fused with computer tomography) may play in managing patients with ectopic thyroid carcinoma. Materials and Methods: A total number of 47 articles were identified on the PubMed and Google Scholar databases, and 3 other articles were selected from articles identified in the references cited in the retrieved articles. After refining the selection, the inclusion and exclusion criteria were applied, resulting in 10 articles that were included in the review. Results: The cases of ectopy included in this review were localised as follows: four cases in the thyroglossal duct, two cases in the mediastinum, one case in the oesophagus, one case in the thorax, one case with a pre-tracheal location, and one case with a latero-cervical location. In all the cases, F-18 FDG PET/CT was used as a diagnostic tool. In one case, F-18 FDG PET/CT was combined with I-131 SPECT/CT and MRI (magnetic resonance imaging). In one case, it was combined with 68 Ga-FAPI PET/CT (Ga-68 radiolabelled FAP inhibitor positron emission tomography fused with computer tomography). The maximum SUVs (standardised uptake values) ranged from 5.5 to 25 g/mL. Conclusions: F-18 PET/CT and I-131 SPECT/CT hybrid nuclear imaging is of great value in assessing ectopic thyroid carcinoma. F-18 FDG PET/CT plays an important role in the primary tumour evaluation and distant disease detection. Ga-68 FAPIs are a promising alternative. I-131 SPECT/CT adds important information related to the anatomical characterization of primary and distant iodine-avid lesions. Full article

Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since the current anthelmintic therapy is no longer sustainable. A better knowledge of the host–parasite interaction is needed to design effective vaccines. To date, few studies have evaluated host–parasite interaction by comparing infected and reinfected animals. The present study evaluates the microscopical hepatic lesions in sheep infected and reinfected with Fasciola hepatica during the acute and chronic stages of infection. The histopathological study revealed the presence of necrotizing foci (NF1) associated with larvae migration during the early stages of infection in the primoinfected (PI) and reinfected (RI) groups. In the late stages of infection of the PI group and at the early and late stages of infection in the RI groups, extensive necrotizing/hemorrhagic foci (NF2) were found in the vicinity of enlarged bile ducts, some containing adult flukes, suggesting parasites may have caused NF2 while feeding. The immunohistochemical study revealed an increase in Foxp3+ T cells in both PI and RI groups with respect to the UC group and in the infiltrates adjacent to NF1 in the RI groups with respect to the PI group, suggesting the F. hepatica induce Foxp3 T cell expansion to facilitate parasite survival. In addition, in both the PI and RI groups, and during acute and chronic stages of the infection, a poor expression of iNOS was found accompanied by a strong expression of CD163, suggesting a marked M2 activation of macrophages in the hepatic lesions, which may be related with healing processes, and it also may facilitate parasite survival. The main differences between PI and RI animals were the more severe infiltration of eosinophils and Foxp3+ T cells, whereas RI did not modify M2 activation of macrophages which occurs since the early stages of primoinfection. Full article

Plate heat exchangers are widely used in the Heating, Ventilation, and Air Conditioning (HVAC) field. Cross-corrugated triangular ducts are commonly employed in plate heat exchangers. Inserting baffles into the cross-corrugated triangular ducts can improve the heat transfer performance of the plate heat exchangers. This study focuses on intricate interdependencies among the flow channel apex angle, the trapezoidal baffle inclination angle, baffle position, and Reynolds number (Re) on heat transfer and pressure drop using response surface methodology (RSM) and computational fluid dynamic (CFD). To identify the factors that maximize the Nusselt number (Nu) and minimize friction factor (f), the RSM is used to design factors, conduct numerical studies, and establish regression equations. The results show that the apex angle, baffle angle, X-direction position, and Re have significantly affected Nu and f. Compared to a non-baffled channel with the same apex angle and Re conditions, the optimized channel enhances heat transfer by 1.54 times and has an almost identical pressure drop. The inclined baffle significantly enhances comprehensive performance at low Re. The synergistic effect of the heat transfer and pressure drop is most optimal when the apex angle of the flow channel is 90°, the trapezoidal baffle inclination angle is 52.5°, and the Re is 1000, with the baffle position at 0.625H in the X-direction. Full article

Biliary atresia (BA) is a poorly understood and devastating obstructive bile duct disease of newborns. Biliatresone, a plant toxin, causes BA-like syndrome in some animals, but its relevance in humans is unknown. To validate the hypothesis that biliatresone exposure is a plausible BA disease mechanism in humans, we treated normal human liver organoids with biliatresone and addressed its adverse effects on organoid development, functions and cellular organization. The control organoids (without biliatresone) were well expanded and much bigger than biliatresone-treated organoids. Expression of the cholangiocyte marker CK19 was reduced, while the hepatocyte marker HFN4A was significantly elevated in biliatresone-treated organoids. ZO-1 (a tight junction marker) immunoreactivity was localized at the apical intercellular junctions in control organoids, while it was markedly reduced in biliatresone-treated organoids. Cytoskeleton F-actin was localized at the apical surface of the control organoids, but it was ectopically expressed at the apical and basal sides in biliatresone-treated organoids. Cholangiocytes of control organoids possess primary cilia and elicit cilia mechanosensory function. The number of ciliated cholangiocytes was reduced, and cilia mechanosensory function was hampered in biliatresone-treated organoids. In conclusion, biliatresone induces morphological and developmental changes in human liver organoids resembling those of our previously reported BA organoids, suggesting that environmental toxins could contribute to BA pathogenesis. Full article

This paper presents a method for predicting atmospheric duct conditions from a clutter power spectrum using deep learning. To accurately predict the duct conditions, deep learning with a binary classification is applied to the proposed refractivity from the clutter (RFC) method. The input data set is the artificial clutter data that are generated via the Advanced Refractive Prediction System (AREPS) simulation software Ver. 3.6 in conjunction with random atmospheric refractive indices. The output of the RFC method is then predicted via binary classification, indicating whether the atmospheric conditions are duct or non-duct. For the cross-validation, the clutter power spectrum data are generated based on real atmospheric refractivity data. The results show that the DNN trained with 5600 pieces of data (validation accuracy of 95.99%) exhibits a binary classification accuracy of 98.36%. The deep neural network (DNN) trained with 28,000 pieces of data (validation accuracy of 98.20%) achieves a binary classification accuracy of 99.06% with an F1-score of 0.9921. Full article