Search Results (134)

Phytochemicals are a potential alternative to antibiotic growth promoters. This study evaluated the effects of phytochemicals (curcuminoids, trans-cinnamaldehyde, and piperine) and monensin on performance and ruminal fermentation during the transition period in grazing dairy cows. In a complete randomized design, 60 Holstein cows (36 multiparous, 24 primiparous; 9 fistulated) were assigned to (1) control (CTL), (2) monensin (MON, 0.30 g/cow/day), or (3) phytochemicals (PHY, 50 g/cow/day) treatment from 30 days prepartum to 60 days postpartum. Prepartum, cows received a total mixed ration (TMR); postpartum, they grazed between a.m. and p.m. milking and were supplemented with TMR. Ruminal fermentation was evaluated at −7, 30, and 60 days postpartum. Prepartum dry matter intake was lower in MON primiparous cows than in CTL and PHY. Additives increased milk yield and lactose percentage in primiparous cows. PHY cows had lower acetate, higher propionate, and reduced acetate-to-propionate and ketogenic-to-glucogenic ratios at 60 days postpartum. MON reduced prepartum protozoa, while PHY increased prepartum branched-chain volatile fatty acids (BCVFAs). Both additives decreased BCVFA and protozoa postpartum. Additives reduced ammonia at 30 days, but only PHY persisted at 60 days. MON and PHY improved primiparous performance, enhanced ruminal fermentation, and promoted glucogenic fermentation while reducing ammonia and protozoa. Full article

Norway spruce (Picea abies (L.) Karst.) has been widely planted beyond its natural range due to its fast growth rate and valuable wood. In Québec, over 200 million seedlings have been planted since 1964. Several of these plantations are now facing a new potential threat, i.e., spruce budworm (Choristoneura fumiferana (Clem.)) infestations. Despite contrasting results, Norway and white spruce (P. glauca [Moench] Voss) apparently sustain a similar degree of budworm defoliation. The main study objective is to quantify defoliation in Norway spruce caused by spruce budworm. We also evaluate the efficacy of Bacillus thuringiensis Berliner spp. kurstaki (Btk) in protecting this exotic host tree. Annual defoliation was assessed in plantations of Norway, white, and black spruce (P. mariana [Mill.] BSP) between 2018 and 2022 in the Bas-Saint-Laurent region. Additional surveys were conducted in Norway and white spruce plantations in the Gaspésie and Côte-Nord to evaluate Btk efficacy. We show that both species exhibit similar defoliation levels, though Norway spruce sometimes sustains greater damage (e.g., 35% vs. 10% in 2019). Btk formulations showed low efficacy in protecting Norway spruce foliage (≥49.32% defoliation in treated plantations). Further studies are needed to understand factors influencing Btk efficacy on this host. Full article

A Capsicum spp.-based phytogenic solution (PHY) improved the performance and thermal tolerance of heat-stressed (HS) growing pigs. Two trials were conducted to further evaluate the HS pig response to supplemental PHY. Trial 1: The effects on the serum concentrations of amino acids (AAs) and the gene expression of tight junction proteins in the jejunum and ileum were assessed with 42 pigs (Landrace-Hampshire-Duroc; 27.0 ± 4.5 kg BW). There were three treatments (14 replicates): pigs under thermoneutral (TN) conditions fed control diet (TN-C); and HS pigs fed control diet without (HS-C) or with PHY (HS-PHY). Trial 2: Two-period digestion trial with eight ileal-cannulated pigs to analyze apparent ileal digestibility (AID) of AAs. Period 1: All TN pigs, fed the control (TN-C) or PHY-supplemented (TN-PHY) diet. Period 2: All HS pigs, fed the same diet as in period 1 (HS-C and HS-PHY). The control diet was based on wheat–soybean meal. In the jejunum, HS-C pigs had reduced occludin gene expression (p < 0.01) compared to TN-C pigs. HS-PHY pigs increased claudin-2 and tight-junction-protein-1 gene expression compared to HS-C (p < 0.05). In the ileum, HS-C and HS-PHY pigs had reduced occludin gene expression (p < 0.01). The serum concentrations of AAs decreased in HS-C compared to TN-C pigs (p < 0.05); except for arginine and isoleucine, HS-PHY pigs partially recovered serum AA levels. HS tended to reduce (p < 0.10; lysine and methionine) and reduced (p < 0.05) the AID of essential AAs. PHY did not mitigate the HS-associated reduced AA digestibility. In conclusion, these results support the concept that a phytogenic solution improves intestinal integrity and partially restores the post-absorption metabolism of amino acids, independent of the amino acid digestibility of heat-stressed pigs. Full article

The wastewater treatment involves various techniques at different technological levels. Treatment takes place in several stages, of which coagulation and flocculation are the most important. Most suspended solids are indeed eliminated during this stage by the addition of a coagulant. In this research, bio-coagulant was extracted from pumpkin seed (PS) waste after extraction of the essential oils, and used with ferric chloride to treat wastewater from the plant of Chalghoum El Aid-Oued El Athmania Mila. In this study, the Box–Behnken design (BBD) with three factors was used to investigate the effect of pH, organic coagulant dosage Pumpkin seed extract (PSE), and chemical coagulant dosage (FeCl₃) on coagulation–flocculation performance in relation to turbidity, chemical oxygen demand (COD), aromatic organic matter (UV 254), and phosphate. The main characteristics of the raw water were turbidity (250 NTU), COD (640 mg/L), UV 254 (0.893 cm⁻¹), and phosphate (0.115 mg/L). The results obtained were very significant. All the statistical estimators (R² ≥ 97% and p ≤ 0.05) reveal that the models developed are statistically validated for simulating the coagulation–flocculation process. It should be noted that the residual values of turbidity, COD, UV 254, and phosphate after treatment by this process were 0.754 NTU; 190.88 mg/L; 0.0028 cm⁻¹; and 0.0149 mg/L, respectively. In this case, the pH, bio-coagulant dosage, and chemical coagulant dosage values were 4; 17.81 mL/L; and 10 mL/L, respectively. In this study, Fourier-transform infrared spectrometer (FTIR) and scanning electron microscope (SEM) characterization of the bio-coagulant proved the presence of the active functional groups responsible for coagulation, namely carboxyl group. Full article

The budding yeast Saccharomyces cerevisiae is a widely utilized model system with myriad applications in terms of industrial, biotechnology, and synthetic biology purposes. One such application is the biosynthesis of commercially and medically important bioactive compounds and their precursors, which oftentimes require culturing conditions at low temperatures to optimize production yield rather than cellular fitness. To lend insight into genetic modifications that may assist this goal, this work focuses on a systematic analysis of the genes that result in an increase in survival following freezing. At present, these genes have been identified in a wide variety of S. cerevisiae wild-type backgrounds—that vary significantly in their properties and behaviors—and in the conditions that led to the annotation of the freeze–thaw survival phenotype. In this work, we report a complete characterization of the thermal tolerance and viability for the freeze–thaw gene family following a standardized protocol within a unified genetic background, the extensively used BY4741 laboratory strain. Our results reveal that five out of these six genes are linked to increased viability in response to both freeze–thaw stress as well as enhanced survival during a heat shock stressor. Follow-up analysis characterized the local spatial effects that gene modification at each locus causes when utilizing the common kanamycin resistance cassette (KanMX6) for the creation of mutant strains and engineering purposes. Full article

Globally, ovarian cancer (OC) is the eighth most common malignant tumour in women. Unfortunately, its symptoms—especially at the early stages—are vague and non-specific, and, thus, most patients are diagnosed at the advanced stages of the disease (stage III and IV) when treatment is not curative. The currently available approved biomarkers are not sufficient for effective screening, prognosis, or monitoring of OC. Liquid biopsy tests such as circulating tumour DNA (ctDNA) analysis has the advantage of monitoring response to treatment in real time and providing a comprehensive genotypic profile of primary, metastatic, and recurrent tumours. Thus, ctDNA analysis can be used as a complementary test for effective diagnosis and monitoring of OC. We comprehensively review current studies (2019–2024) on OC, critically highlighting recent developments and applications of ctDNA for the diagnosis and management of the disease. Full article

This study investigated biomarkers of fumonisin exposure in pigs fed diets contaminated with fumonisins at the European Union’s maximum recommended level. Pigs were assigned to either a fumonisin (FB) diet or a fumonisin plus AlgoClay (FB + AC) diet for durations of 4, 9, and 14 days. At 14 days, the plasma Sa1P:So1P ratio increased in pigs fed the FB diet, while the Sa:So ratio remained unchanged. In the liver, FB1 was detected at four days of exposure, with the concentration tending to increase through day 14. The Sa:So and C22-24:C16 ratios of 18:1-, 18:2-, and m18:1-ceramides were elevated at 9 and 14 days, respectively. In the kidneys, FB1 was only detectable at 14 days, and the Sa:So and C22-24:C16 ratios of 18:1-ceramides were increased. In both the liver and kidneys, the increase in the C22-24:C16 ratio was attributed to a reduction of C16 ceramides. In the lungs, no FB1 was detected; however, the Sa:So and Sa1P:So1P ratios increased, and C16 ceramide concentrations decreased at 14 days. Feeding the pigs the FB + AC diet resulted in a reduction of the FB1 tissue-to-feed ratio in the liver and kidneys but did not affect the Sa:So or Sa1P:So1P ratios. Interestingly, the decreases in C16 ceramides observed in the FB diet group were no longer detectable in the FB + AC group. Overall, these findings highlight the complexity of the relationship between FB1 tissue concentrations and sphingolipid changes, suggesting that a comprehensive analysis of multiple biomarkers is required to fully understand fumonisin’s effects. Full article

Curve squeal is a highly disturbing tonal noise produced by railway vehicles on tight curves, primarily attributed to lateral sliding at the wheel–rail interface. An essential step to estimate curve squeal noise levels is to determine the nonlinear self-sustained vibrations, for which time integration is a commonly used method. However, although it is known that the initial conditions affect the solutions obtained with time integration, their impact on the limit cycles is often overlooked. This study investigates this aspect for a curve squeal model based on falling friction and a modal reduction of the wheel and provides some insights on the mode competition phenomena and the nature of the final limit cycles obtained. The paper first details the curve squeal model, stability analysis, as well as the initial condition derivation, and then discusses the time integration and limit cycle results in both time and frequency domains. The results reveal two primary families of limit cycles that can be obtained for both types of initial conditions. The cases where stationary vibrations result in a quasi-periodic regime converge to a unique limit cycle which displays three fundamental frequencies corresponding to specific wheel modes, plus harmonic interactions among them. Full article

Low transitive constructions are ubiquitous in English conversation and as such can be considered “typical” clauses. This article furthers this claim by showing that these constructions are also most frequent in a different genre: arguments between participants at organized protests. It has been argued that one reason these constructions are so frequent is that they function to display participants’ stances. Arguments are a type of interaction where stance displays abound. In fact, they are defined as sequences of utterances that display opposing stances. Thus, the study goes on to examine how the most frequent of the low transitive constructions in the data—clauses with the nonverbal predicate be—function to display opposition across utterances. Du Bois has analyzed stance as resonance across utterances, created from structural parallelism, and he argues that slight changes between linguistic forms can create differing focal points that index contrasts. This framework is used to analyze how participants use the multiple semantic functions of be clauses across interactional sequences to display and modify their stances in response to their opponent. It is suggested that the versatility and ambiguity of be clauses are especially useful in arguments where participants do not share a set of common beliefs. Full article

This study evaluated the potential of using raw Maclura pomifera and wild carob for the treatment of methylene blue (MB) and crystal violet (CV) as part of the search for new, abundant, and cost-effective natural materials applicable for wastewater treatment. Additionally, it explored the impact of washing water temperature on the adsorption performance of these raw organic materials. The physicochemical properties of the materials were characterized using BET, SEM/EDS, and FTIR analyses. The effects of various experimental parameters were investigated through batch adsorption experiments. The results demonstrated that the influence of washing water temperature was material-dependent. For Maclura pomifera, the maximum adsorption capacity of methylene blue and crystal violet decreased from 134.4 and 136.6 mg g⁻¹ for MPC to 67.1 and 90.5 mg g⁻¹ for MPH. In contrast, the adsorption capacities of wild carob biosorbents remained consistent, with the maximum amounts adsorbed for methylene blue and crystal violet by CC, CW, and CH being close, around 78.8 and 98.9 mg g⁻¹, respectively, indicating a minimal effect of washing temperature on this material. The adsorption of both dyes onto the adsorbents was positively affected by increasing the pH, contact time, and initial dye concentration and was negatively affected by increasing adsorbent dose or ionic strength. Adsorption isotherms and kinetics were modeled using various mathematical approaches. The kinetic data were accurately described by a pseudo-second-order model, with a significant contribution from intraparticle diffusion. The Sips and Redlich–Peterson models provided the best fit for the adsorption isotherms of both dyes on the biosorbents. These findings confirm that the selected biomaterials are excellent adsorbents for the removal of cationic dyes. Full article

The advancement of Arrival MANager (AMAN) is crucial for addressing the increasing complexity and demand of modern airspace. This study evaluates the operational feasibility and effectiveness of an innovative AMAN designed for en route airspace, the so-called En Route AMAN. The En Route AMAN functions as a controller support system, facilitating the sharing of information between en route air traffic controllers (ATCos), approach controllers (current AMAN), and airport controllers (Departure Managers) in airports with multiple runways. The En Route AMAN aims to support upstream ATCos by sequencing and spacing of incoming streams via speed control and runway assignment, thereby enhancing overall air traffic efficiency. Human-In-The-Loop simulations involving rated ATCos are performed under scenarios that replicate real-world traffic and weather conditions. These simulations focus on upstream airspace to assess the impact of En Route AMAN on delay mitigation and ATCos’ performance. Unlike previous studies that solely relied on theoretical models and fast-time simulation for operational feasibility evaluation, this approach incorporates ATCos’ real-time decision-making, situational awareness, and task management, addressing critical operationalization challenges. The results demonstrated that the En Route AMAN could reduce the average flight duration by up to 25.6 s and decrease the total number of ATCo instructions by up to 20% during peak traffic volume. These findings support that the En Route AMAN is both operationally viable and effective in mitigating arrival delays, highlighting the importance of Human-In-The-Loop for practical validation. Full article

Today’s growing demand for lithium-ion batteries across various industrial sectors has introduced a new concern: battery aging. This issue necessitates the development of tools and models that can accurately predict battery aging. This study proposes a general framework for constructing battery aging models using machine learning techniques and compares these models with two existing empirical models, including a commercial one. To build the models, the databases produced by EVERLASTING and Bills et al. were utilized. The aim is to create universally applicable models that can address any battery-aging scenario. In this study, three types of models were developed: a vanilla neural network, a neural network inspired by extreme learning machines, and an encoder coupled with a neural network. The inputs for these models are derived from established knowledge in battery science, allowing the models to capture aging effects across different use cases. The models were trained on cells subjected to specific aging conditions and they were tested on other cells from the same database that experienced different aging conditions. The results obtained during the test for the vanilla neural network showed an RMSE of 1.3% on the Bills et al. test data and an RMSE of 2.7% on the EVERLASTING data, demonstrating similar or superior performance compared to the empirical models and proving the ability of the models to capture battery aging. Full article

This study evaluates the use of remote sensing data to improve the understanding of groundwater resources in climate-sensitive regions with limited data availability and increasing agricultural water demands. The research focuses on estimating groundwater reserve dynamics in two major river basins in Morocco, characterized by significant local variability. The study employs data from Gravity Recovery and Climate Experiment satellite (GRACE) and ERA5-Land reanalysis. Two GRACE terrestrial water storage (TWS) products, CSR Mascon and JPL Mascon (RL06), were analyzed, along with auxiliary datasets generated from ERA5-Land, including precipitation, evapotranspiration, and surface runoff. The results show that both GRACE TWS products exhibit strong correlations with groundwater reserves, with correlation coefficients reaching up to 0.96 in the Oum Er-rbia River Basin and 0.95 in the Tensift River Basin (TRB). The root mean square errors (RMSE) were 0.99 cm and 0.88 cm, respectively. GRACE-derived groundwater storage (GWS) demonstrated a moderate correlation with observed groundwater levels in OERRB (R = 0.59, RMSE = 0.82), but a weaker correlation in TRB (R = 0.30, RMSE = 1.01). On the other hand, ERA5-Land-derived GWS showed a stronger correlation with groundwater levels in OERRB (R = 0.72, RMSE = 0.51) and a moderate correlation in TRB (R = 0.63, RMSE = 0.59). The findings suggest that ERA5-Land may provide more accurate assessments of groundwater storage anomalies, particularly in regions with significant local-scale variability in land and water use. High-resolution datasets like ERA5-land are, therefore, more recommended for addressing local-scale heterogeneity in regions with contrasted complexities in groundwater storage characteristics. Full article

Salivary gland pleomorphic adenoma (SGPA) is the most common type of benign epithelial tumor; it is observed more commonly in females (with a female-to-male ratio of 1.43:1), and the age at diagnosis ranges between 40 and 59 years, with only 2% of cases diagnosed before age 18. Cri du Chat (CdC) is a rare syndrome caused by deletions of various sizes in the short arm of chromosome 5. Tumors in CdC patients are extremely rare: in Danish, Spanish, Australian, and Japanese groups of cases, no tumors have been reported, while a few cases have been described among 321 CdC patients collected in Italy and Germany. These cases all involve tumors that appear at a young age. We here report the case of a parotid pleomorphic adenoma in an 8-year-old boy with CdC. Exome analysis did not identify variants certainly significant for the development of SGPA. A CGH array, analyzed both in peripheral blood and tumor samples, failed to recognize anomalies previously associated with SGPA but identified a de novo duplication in 7p15.2, which contains part of a gene, SKAP2, in which the increased copy number is associated with the development of a different type of tumor such as pancreatic duct adenocarcinoma. The assumption that the duplication in 7p15.2 is relevant for the development of SGPA in our patient implies that CGH array studies must be included early in life in routine work-ups of CdC to identify CNVs with possible pathogenic roles for tumor development. This is particularly also relevant in relation to the severely impaired possibility for patients with CdC to report discomfort or pain related to tumor development. Constitutional CNVs in addition to the deletion in 5p should also be extensively studied to verify if their presence in some patients could explain why, in these cases, tumors develop at an age younger than expected. Full article

Against the backdrop of global decolonization, nationalist movements, and civil upheavals in the early and mid-20th century, a renewed form of cosmopolitanism emerged through the intercultural encounters between African American and Chinese intellectuals. This cosmopolitan ideal was cultivated and embodied by these two historically, culturally, and geographically distinct communities and ultimately exerted lasting influences on a global scale. Despite initially perceiving China as a distant Other, African American authors such as Langston Hughes and W.E.B. Du Bois made their journeys to China in search of cultural inspiration for literary creations and social endeavors. While actively promoting the works of African American authors in China, the Chinese intellectual community in turn viewed the African American people as the Other Self and potential allies in international affairs. Mutual understanding and appreciation were pursued from both sides, leading to a co-reinvention of cosmopolitan ethos. By delving into the interconnected narratives, this article seeks to elucidate the nuanced dynamics and reciprocal influences that characterized the Sino–African American intellectual relationships in the context of international solidarity, decolonization, and the quest for social justice in the early and mid-20th century. Full article