Search Results (144)

Understanding the genetic basis of agronomic traits in quinoa adapted to the Qinghai–Tibet Plateau is essential for developing high-yield cultivars, as conventional breeding is constrained by limited molecular tools. In this study, 300 cultivated accessions were evaluated for five quantitative traits, and whole-genome resequencing generated 3.69 million high-quality SNPs. Population structure analysis and genome-wide association study (GWAS) were conducted, with integration of seed developmental transcriptomes to refine trait-associated loci. A highly admixed genetic background (K = 7) was revealed, and 11 significant QTLs across seven chromosomes were identified, involving genes related to metabolism, transport, and cell-wall formation. Among these, CesA4 (CQ042210) showed a strong association with thousand grain weight (TGW) and a distinct expression maximum at the early seed-filling stage. These results provide a genomic framework for understanding trait variation in plateau-adapted quinoa and highlight promising targets for marker-assisted breeding. Full article

The aim of this study was to examine the relationship between Cultural Intelligence (CI) and Intercultural Sensitivity (IS) and to test the mediating role of Internationalisation in Higher Education (IHE). A correlational survey design was employed with full-time academics in the Turkish Republic of Northern Cyprus (N = 391). Standardised instruments were administered: the Cultural Intelligence Scale (CQS), the Intercultural Sensitivity Scale (ISS), and the Internationalisation Perception Scale for Academics (IPSA). Construct validity and reliability were verified via confirmatory factor analysis, and the structural model was estimated using structural equation modelling (SEM) in SPSS–AMOS. The analysis revealed that CI exerts a positive and statistically significant effect on IS. CI was also found to be positively associated with IHE, and IHE demonstrated a positive and significant effect on IS. Mediation testing indicated that IHE functions as a significant partial mediator of the CI–IS relationship. Robustness checks with control variables showed that academic rank and faculty type have small but significant positive associations with IS. Overall, the findings suggest that the development of CI among academic staff directly enhances intercultural responsiveness and, additionally, strengthens IS through engagement with internationalisation processes. The results provide practical guidance for universities seeking socially sustainable internationalisation, indicating that institution-level strategies that embed intercultural learning and support academics’ international engagement may amplify the translation of CI into demonstrable intercultural sensitivity. Full article

This study investigated the effects of alternative sweeteners, allulose (AL), stevia (ST), and xylose (XY), on the physicochemical, antioxidant, textural, and sensory properties of Yanggaeng fortified with Cissus quadrangularis (CQ) powder. Replacing sucrose (SU) with alternative sweeteners significantly affected the proximate composition and overall quality of Yanggaeng. Formulations containing AL, ST, or XY exhibited higher moisture retention and lower carbohydrate content than those containing SU. Colorimetric analysis revealed that Yanggaeng prepared with alternative sweeteners developed a darker coloration and greater browning intensity, likely due to enhanced Maillard reactions during heating. Among the sweeteners tested, AL showed the highest total phenolic content and relatively high antioxidant activity, suggesting potential functional advantages beyond sweetness. Textural analysis indicated that ST enhanced gel strength and elasticity even under high-moisture conditions, whereas AL produced a softer texture, which may be desirable for products requiring reduced firmness. Although consumer preference scores did not differ significantly across most sensory attributes, both ST and AL achieved acceptable overall profiles, with sweetness ratings comparable to those of SU. These findings suggest that CQ-enriched Yanggaeng sweetened with alternative sweeteners can be developed as a promising low-sugar dessert option without compromising quality or consumer acceptability. Full article

The high use and improper disposal of chloroquine (CQ) during the COVID-19 pandemic have significantly increased its presence in water bodies, representing an environmental risk. Adsorption is one of the most-used treatments to remove recalcitrant compounds, although there is still a lack of efficient biosorbents. This work aimed to develop an efficient biosorbent using additive manufacturing (AM) to synthesize bionanocomposite hydrogels based on cellulose fibers, sodium alginate (SA), and graphene oxide (GO) for CQ adsorption. The hydrogels were characterized by mechanical, morphological, and physicochemical techniques. Results show that increasing GO content and reducing water contributed to higher yield stress, which is important for maintaining shape fidelity during the printing. SEM images evidenced thin GO layers interacting with the polymer matrix and cellulose fibers, resulting in 3D disordered porous microstructures. The adsorption capacity of the 3D-printed hydrogel samples for aqueous CQ was analyzed by evaluating the pH effect, contact time, and the adsorption equilibrium isotherms, showing notorious potential for CQ removal, with maximum adsorption capacity of ~25 mg∙g⁻¹ at 25 °C. Results show that the tested formulations were stable for producing hydrogels and efficient on chloroquine adsorption, revealing their potential as novel adsorbents for removing emerging organic pollutants from water. Full article

Thioester-containing proteins (TEPs), which are distinguished by the thioester motif (GCGEQ), are essential to arthropods’ defense against infections. Although TEPs have been extensively investigated in Anopheles, Aedes, and Drosophila, their functions in Culex mosquitoes remain inadequately explored. Interestingly, we discovered that Culex TEPs exhibit functional antagonism to their orthologs in other species, actively facilitating viral infection in this vector. In this study, we identified nine TEP genes in Culex quinquefasciatus, three of which were found to critically facilitate Japanese encephalitis virus (JEV) infection, with CqTEP27 exhibiting the most pronounced proviral effect. Mechanistically, CqTEP27 may have suppressed the production of several antimicrobial peptides (AMPs), which increased JEV replication. Our work also highlights the potential of targeting susceptibility factors such as CqTEP27 to block pathogen acquisition. Notably, the rate of mosquito infection was significantly decreased by membrane blood feeding antisera against CqTEP27. Therefore, vaccination against CqTEP27 offers a workable method of avoiding JEV infection. According to our research, CqTEP27 is a promising target for the development of vaccines that prevent JEV transmission. By preventing viral infection in mosquitoes that feed on immunized hosts, this approach can directly disrupt the natural transmission cycle, offering a novel strategy to reduce the disease burden. Full article

Quantum machine learning (QML) integrates quantum computing with classical machine learning. Within this domain, QML-CQ classification tasks, where classical data is processed by quantum circuits, have attracted particular interest for their potential to exploit high-dimensional feature maps, entanglement-enabled correlations, and non-classical priors. Yet, practical realizations remain constrained by the Noisy Intermediate-Scale Quantum (NISQ) era, where limited qubit counts, gate errors, and coherence losses necessitate frugal, noise-aware strategies. The Data Re-Uploading (DRU) algorithm has emerged as a strong NISQ-compatible candidate, offering universal classification capabilities with minimal qubit requirements. While DRU has been experimentally demonstrated on ion-trap, photonic, and superconducting platforms, no implementations exist for spin-based quantum processing units (QPU-SBs), despite their scalability potential via CMOS-compatible fabrication and recent demonstrations of multi-qubit processors. Here, we present a pulse-level, noise-aware DRU framework for spin-based QPUs, designed to bridge the gap between gate-level models and realistic spin-qubit execution. Our approach includes (i) compiling DRU circuits into hardware-proximate, time-domain controls derived from the Loss–DiVincenzo Hamiltonian, (ii) explicitly incorporating coherent and incoherent noise sources through pulse perturbations and Lindblad channels, (iii) enabling systematic noise-sensitivity studies across one-, two-, and four-spin configurations via continuous-time simulation, and (iv) developing a noise-aware training pipeline that benchmarks gate-level baselines against spin-level dynamics using information-theoretic loss functions. Numerical experiments show that our simulations reproduce gate-level dynamics with fidelities near unity while providing a richer error characterization under realistic noise. Moreover, divergence-based losses significantly enhance classification accuracy and robustness compared to fidelity-based metrics. Together, these results establish the proposed framework as a practical route for advancing DRU on spin-based platforms and motivate future work on error-attentive training and spin–quantum-dot noise modeling. Full article

Quinoa (Chenopodium quinoa Willd.) is a highly nutritious crop known for its tolerance to salt stress; however, the molecular mechanisms underlying this trait remain poorly understood. This study aims to perform the in silico characterisation of calcium-dependent protein kinase (CPK) gene family sequences and to evaluate their expression profiles under salt stress conditions. Using bioinformatics tools, CPK family gene sequences were identified and in silico-characterised, including conserved domains, cis-regulatory motifs, and physicochemical properties. Experimentally, two contrasting accessions were compared: a salt-tolerant one (UNSA_VP033) and a salt-sensitive one (UNSA_VP021). Salt tolerance indices were determined during germination, gene expression levels were quantified by RT-qPCR, and antioxidant enzyme activities, along with malondialdehyde (MDA) content, were evaluated under different NaCl concentrations. Sixteen sequences with characteristic CPK family domains were identified. Promoter analysis revealed cis-elements associated with hormonal and stress responses. Physicochemical parameters predicted proteins of 50–60 kDa with variable isoelectric points. Experimentally, UNSA_VP033 showed the significant overexpression of CqCPK12, CqCPK17, CqCPK20, and CqCPK32, correlated with the higher antioxidant activity of superoxide dismutase (SOD) and peroxidase (POD), and lower MDA levels at 200 mM NaCl. In contrast, the sensitive accession exhibited significant reductions in gene expression and antioxidant activity. In conclusion CPK genes play a key role in the salt stress response in quinoa, particularly CqCPK12, CqCPK17, CqCPK20, and CqCPK32 in the tolerant accession. These findings may contribute to the development of more salt-tolerant varieties, thereby enhancing agricultural sustainability in saline soils. Full article

Sex-specific markers are important basic tools for the sex-controlled breeding of farmed fish. Here, we aimed to develop a rapid yet accurate, cost-effective method for determining the genetic sex of the Chinese hooksnout carp (Opsariichthys bidens), a freshwater fish. Using whole-genome resequencing technology, along with bulked segregant analysis (BSA) and chromosome quotient (CQ) methods, sex-specific regions were screened, and corresponding primers were designed to validate the screening results. A total of 45 sex-specific regions were successfully screened through BSA sequencing and CQ analysis, and 50 pairs of primers were designed for use in the screening verification. The Mar28 primer pair showed stable sex specificity in multiple populations of O. bidens, accurately distinguishing male from female individuals. This primer pair amplified two bands (509 and 814 bp) in males, but only one band (509 bp) in females. The genetic sex identification method established here provides a theoretical basis for studying the mechanism of sex determination in O. bidens, has implications for the monosex culture and molecular breeding of O. bidens, and has significant scientific and practical value. Full article

Background/Objectives: Conventional therapeutic strategies exhibit limited efficacy against pancreatic cancer, primarily due to its profoundly hypoxic tumor microenvironment and dense fibrotic stroma. Chemodynamic therapy (CDT) holds promise; however, its application in pancreatic cancer is restricted by insufficient endogenous hydrogen peroxide (H₂O₂) levels and the activation of protective autophagy in response to oxidative stress. Methods: To overcome these obstacles, we developed a tumor microenvironment-responsive, pancreatic cancer-targeted CDT nanoamplifier—H-MnO₂/GOX&CQ-iRGD—comprising a hollow mesoporous MnO₂ shell co-loaded with glucose oxidase (GOX) and chloroquine (CQ), and surface-functionalized with the tumor-penetrating peptide iRGD. GOX catalyzes glucose oxidation to generate H₂O₂, enhancing Fenton-like reactions. CQ suppresses autophagy induced by oxidative stress, thereby alleviating therapy resistance. The iRGD peptide targets integrin α_vβ₃, which is overexpressed on pancreatic cancer cells and tumor vasculature, promoting deep tumor penetration and enhanced delivery efficiency. Results: We comprehensively characterized the nanoplatform’s physicochemical properties, tumor microenvironment triggered degradation, controlled drug release, glucose-driven H₂O₂ generation, and hydroxyl radical production in vitro. Cellular studies assessed nanoparticle uptake, intracellular H₂O₂ production, autophagy inhibition, and cytotoxicity. In vivo experiments further demonstrated effective tumor targeting and significant therapeutic outcomes in pancreatic cancer models. Conclusions: This nanoplatform addresses major barriers of CDT—namely, insufficient H₂O₂ levels, autophagy-mediated resistance, and limited intratumoral penetration—offering a promising strategy for pancreatic cancer treatment. Full article

Background/Objectives: The global spread of Mycoplasma pneumoniae (MP) poses a significant threat to public health; however, no licensed vaccine for human use is currently available. The development of a safe and effective vaccine is a critical priority. This study systematically evaluated the protective efficacy and safety of an inactivated MP vaccine using different adjuvants and immunization routes. Methods: Mice were immunized with inactivated vaccines via either intramuscular (IM) injection with aluminum hydroxide (alum) or a combination of CpG+QS21 (CQ) or via intranasal (IN) administration of Flagellin from Salmonella Typhimurium (FLA-ST), a potent Toll-like receptor 5 (TLR5) agonist, as a mucosal adjuvant. Vaccine-induced immunogenicity, protective efficacy against MP challenge, and associated lung pathology were assessed. Results: Both IM-vaccinated groups (alum and CQ) exhibited robust systemic immune responses. However, upon subsequent MP challenge, these groups exhibited significant inflammatory pathology in the lung tissues. Notably, the CQ-adjuvanted group displayed severe pulmonary inflammatory infiltration. In stark contrast, compared with the IM-vaccinated group, the IN-immunized group with the FLA-ST mucosal adjuvant achieved significant clearance of MP from the lungs and showed markedly milder histopathological lung damage. Conclusions: Our findings suggest that IM immunization with CQ-adjuvanted inactivated vaccines may represent a suboptimal strategy for MP, given the risk of exacerbating lung immunopathology. Conversely, a mucosal immunization approach using the FLA-ST adjuvant demonstrates considerable promise, offering an effective balance between bacterial clearance and an improved safety profile, highlighting its potential for future MP vaccine development. Full article

Heat shock protein 90 (HSP90) is an evolutionarily conserved molecular chaperone. Numerous studies have shown that it is widely involved in protein folding, assembly, stabilization, activation, and degradation in response to various biotic and abiotic stresses in many normal cellular processes and under stress conditions. We identified 11 members of the CqHSP90 gene family in the quinoa (Chenopodium quinoa) genome by bioinformatics analysis. Phylogenetic tree analysis showed that quinoa was more closely related to dicotyledonous plants than to monocotyledonous plants. Quinoa is susceptible to high-temperature stress during its growth and development. We analyzed the cis-acting elements of its promoter, and found that nearly 1/3 of the cis-acting elements were stress-responsive, and 2/3 of them had heat-responsive elements. The results of qRT-PCR showed that heat shock at 40 °C could induce a high expression of CqHSP90.1c and CqHSP90.6a. Subcellular localization indicates that they are all membrane proteins. At the same time, both CqHSP90.1c and CqHSP90.6a overexpression lines improved the tolerance of Arabidopsis thaliana under high temperature, indicating that both of them had a positive regulatory effect under heat stress. Results of this study could provide useful information for further study on the biological role of CqHSP90.1c and CqHSP90.6a, and provide theoretical basis for quinoa resistance breeding. Full article

Background: Zebrafish-based drug discovery systems provide significant advantages over mammalian models for high-throughput in vivo screening. Among these, the NF-κB eGFP reporter system significantly enhances drug discovery in zebrafish by enabling real-time, high-resolution monitoring of pathway activity in live organisms, thereby streamlining mechanistic studies and high-throughput screening. Methods: We developed a novel AI (Quantifish and Orange software)-based zebrafish precision individualized 96-well ZF plates (0–7 dpf) and individualized MT tanks (8 dpf–4 mpf) protocol for the transposon-mediated transgenesis of the NFκB eGFP reporter system. Results: One-cell stage embryos were administered NFκB reporter construct and Tol2 transposase mRNA via microinjection and transferred to separate wells of a 96-well ZF plate. Bright-field and fluorescence images of each well were captured at 5 dpf in the F0, F1, and F2 generations using the automated confocal high-content imager CQ1. The Quantifish software was used for the automated detection and segmentation of zebrafish larval fluorescence intensity in specific regions of interest. Quantitative data on the fluorescence intensity and distribution patterns were measured in Quantifish, and advanced statistical and machine learning methods were applied using Orange. Imaging data with eGFP expression results were assessed to evaluate the efficiency of the transgenic protocol. Discussion: This AI-enhanced precision protocol allows for high-throughput screening and quantitative analysis of NFκB reporter transgenesis in zebrafish, enabling the efficient identification and characterization of stable transgenic lines that exhibit tissue-specific expression of the NF-κB reporter, such as lines with induced expression restricted to the retina following LPS stimulation. This approach streamlines the evaluation of regulatory elements, enhances data consistency, and reduces animal use, making it a valuable tool for zebrafish drug discovery. Full article

General large language models (LLMs) often suffer from semantic misinterpretation, information redundancy, and hallucinated content when applied to educational question-answering tasks. These issues hinder their effectiveness in supporting students’ specialized course learning and self-directed study. To address these challenges, this study proposes an intelligent tutoring model that integrates a knowledge graph with a large language model (KG-CQ). Focusing on the Data Structures (C Language) course, the model constructs a course-specific knowledge graph stored in a Neo4j graph database. It incorporates modules for knowledge retrieval, domain-specific question answering, and knowledge extraction, forming a closed-loop system designed to enhance semantic comprehension and domain adaptability. A total of 30 students majoring in Educational Technology at H University were randomly assigned to either an experimental group or a control group, with 15 students in each. The experimental group utilized the KG-CQ model during the answering process, while the control group relied on traditional learning methods. A total of 1515 data points were collected. Experimental results show that the KG-CQ model performs well in both answer accuracy and domain relevance, accompanied by high levels of student satisfaction. The model effectively promotes self-directed learning and provides a valuable reference for the development of knowledge-enhanced question-answering systems in educational settings. Full article

The Cultural STEM Night (CSN) initiative was developed to address the persistent lack of culturally relevant STEM teaching materials, which often contributes to student disengagement—particularly among underrepresented populations. This study examined the impact of the CSN program on enhancing STEM affinity and cultural intelligence (CQ) among American and Korean teacher candidates. Over six weeks, participants engaged in synchronous workshops, virtual cultural exchanges, and collaborative STEM lesson design integrating Korean cultural contexts. Quantitative analysis of pre- and post-program surveys using the STEM Affinity Test and Cultural Intelligence Scale revealed statistically significant improvements across all subdomains of STEM affinity (identity, interest, self-concept, value, and attitudes) and in most dimensions of CQ (metacognitive, cognitive, and behavioral). However, motivational CQ did not show significant gains, likely due to limited student interaction time during the event. Qualitative data from written reflections and focus group discussions supported these findings, indicating increased instructional adaptability, cultural awareness, and confidence in designing inclusive STEM lessons. These results demonstrate the transformative potential of interdisciplinary, culturally immersive programs in teacher education. The CSN model, supported by digital collaboration tools, offers a scalable and effective approach to preparing educators for diverse classrooms. Findings underscore the importance of integrating culturally responsive teaching into STEM education to promote equity, engagement, and global competence. Full article

This study establishes a covalently anchored wettability alteration strategy for enhanced oil recovery (EOR) using perfluorinated siloxane (CQ), addressing limitations of conventional modifiers reliant on unstable physical adsorption. Instead, CQ forms irreversible chemical bonds with rock surfaces via Si-O-Si linkages (verified by FT-IR/EDS), imparting durable amphiphobicity with water and oil contact angles of 135° and 116°, respectively. This modification exhibits exceptional stability: increasing salinity from 2536 to 10,659 mg/L reduced angles by only 6° (water) and 4° (oil), while 70 °C aging in aqueous/oleic phases preserved amphiphobicity without reversion—supported by >300 °C thermal decomposition in TGA; confirming chemical bonding durability. Mechanistic analysis identifies dual EOR pathways: amphiphobic surfaces lower rolling angles, surface free energy (SFE), and fluid adhesion to facilitate pore migration, while CQ intrinsically reduces oil-water interfacial tension (IFT). Core displacement experiments showed that injecting 0.05 wt% CQ followed by secondary waterflooding yielded an additional 10–18% increase in oil recovery. This improvement is attributed to enhanced mobilization of residual oil, with greater EOR efficacy observed in smaller pore throats. Field trials at the Huabei Oilfield validated practical applicability: Production rates of test wells C-9 and C-17 increased several-fold, accompanied by reduced water cuts. Integrating fundamental research, laboratory experiments, and field validation, this work systematically demonstrates a wettability-alteration-based EOR method and offers important technical insights for analogous reservoir development. Full article