Search Results (27)

The aim of this study was to conduct a comparative analysis of the effects of native quinoa grain with a high saponin content and quinoa grain subjected to preliminary saponin removal with low saponin content on growth, meat quality, biochemical blood composition, and the expression of genes related to muscle growth, gut health, and nutrient transport in broiler chickens. The control group of chickens received a standard diet. The SAP group feed contained quinoa grain without saponin removal (saponin level—5.20%) at 3% of the “Starter” feed mass and 5% of the “Grower” and “Finisher” feeds, maintaining the same nutritional values as the control group. The SAP-FREE group feed contained quinoa grain that was pre-treated to remove saponins by washing with water for 60 min at a temperature of 50 °C (saponin level—0.24%) in the same amount as the SAP group. The research results indicated certain advantages of unprocessed quinoa grain in relation to saponin content. Specifically, in the SAP group, the broiler performance index was at the same level as the control, while the SAP-FREE group had a high mortality rate (10%), resulting in a performance index that was 23.82 units lower than the control. The use of quinoa grain with high saponin content promoted better development of thigh muscles by 9.6% compared to the control (p = 0.008) and increased yields of wing, neck, and back muscles by 2.9 abs.% (p = 0.007) compared to the use of purified quinoa grain. The fat yield decreased by 1.7 abs.% (p = 0.015) with saponin-free quinoa compared to the control and by 2% (p = 0.008) compared to the high saponin group, making this feeding system viable for producing dietary meat. Upon stopping the feeding of purified quinoa, chickens showed a 34.0% increase in AST activity (p = 0.019) and a 15.7% increase in creatinine levels (p = 0.008), likely indicating intensified protein metabolism upon cessation of the inhibiting factor of purified quinoa. Molecular genetic studies revealed a 1.6-fold increase in IGF1 gene expression (p = 0.014) in breast muscle and a 69.12-fold increase (p = 0.010) in AvBD9 in the cecum due to high-saponin quinoa grain, while purified quinoa increased GHR gene expression by 3.29 times (p = 0.039) in breast muscle and decreased IRF7 activity to 2^−ΔΔCT = 0.54 (p = 0.017). The expression of transporter protein genes decreased to low or undetectable levels, indicating the presence of anti-nutritional factors and the need for further research on feeding quinoa with the addition of proteases. Thus, high-saponin quinoa grain, unlike purified quinoa, positively influences gut health and bird survival, maintaining performance levels similar to the control, suggesting the feasibility of using unprocessed quinoa in poultry nutrition, thus avoiding additional costs in feed preparation. Full article

This paper presents MS-MTSA, a multi-scale multi-type self-attention network designed to enhance AV1-compressed video through targeted post-filtering. The objective is to address two persistent artifact issues observed in our previous MTSA model: visible seams at patch boundaries and grid-like distortions from upsampling. To this end, MS-MTSA introduces two key architectural enhancements. First, multi-scale block-wise self-attention applies sequential attention over 16 × 16 and 12 × 12 blocks to better capture local context and improve spatial continuity. Second, refined patch-wise self-attention includes a lightweight convolutional refinement layer after upsampling to suppress structured artifacts in flat regions. These targeted modifications significantly improve both perceptual and quantitative quality. The proposed network achieves BD-rate reductions of 12.44% for Y, 21.70% for Cb, and 19.90% for Cr compared to the AV1 anchor. Visual evaluations confirm improved texture fidelity and reduced seam artifacts, demonstrating the effectiveness of combining multi-scale attention and structural refinement for artifact suppression in compressed video. Full article

This study aimed to evaluate how natural dietary supplements, including essential oils (EOs) and probiotics, influence the growth performance, carcass traits, serum components, gut function, gene expression, and jejunal histomorphology of growing quails. A total of 240 unsexed 7-day-old growing Japanese quails were randomly assigned to four experimental groups (n = 60 per group), with each group further divided into six replicates (10 quails per replicate). The control group (S₀) received a basal diet without incorporating any additives, while the experimental groups were supplemented with (i) essential oils (S₁); (ii) probiotics (S₂); or (iii) a mixture of EOs and probiotics (S₃) at a level of 1.5 kg/ton and 0.55 g per kg diet, respectively, and the ratio of the mixture of EOs and probiotics was approximately 2.73:1. The results showed that, from 7 to 35 days of age, S₃ quails showed increased growth performance, carcass weight, and serum total protein with a decreased lipid profile, outperforming the individual supplementation of either additive (p < 0.05). Importantly, EOs or probiotics enhanced immune-antioxidant status in growing quails, particularly those who were fed both EOs and probiotics, showing significantly increased levels of the serum immune parameters IgY and IgM as well as boosting T-AOC, SOD, and GPx levels when MDA content was lowered compared to S₀ quails (p < 0.05). Additionally, in quails fed a mixture of EOs and probiotics, the primary pro-inflammatory factors TNF-α, IL-1β, and IL-6 were downregulated, and the anti-inflammatory factors TGF-β and IL-10 were elevated compared to the S₀ group (p < 0.05). In this context, there was a notable increase in growth (IGF-I, myogenin, and AvUCP), immunological (IL-2, IL-4, IL-6, and AVBD), antioxidant (SOD, CAT, GPx, and ATOX1), and intestinal absorption (VEGF, MUC2, GLUT2, calbindin, and FABP6) markers in quails supplemented with EOs and/or probiotics when compared to the control group (p < 0.05). The combination of EOs and probiotics had the most noticeable impact on the markers’ expression patterns compared to either additive alone (p < 0.05). Consistent with our results, quails given both EOs and probiotics showed significantly greater villi in terms of height and width when compared to the control group in intestinal histomorphology, the primary measure of intestinal wellness. In conclusion, quail diets could benefit from the use of EOs or probiotics as natural growth promoters since they improve growth performance, blood parameters associated with protein and lipid profiles, immune-antioxidant status and inflammation, and marker gene expression profiles of growth, immune, antioxidant, and intestinal absorption. Full article

(1) The aim of this study was to elucidate the role of the Gallus gallus avian β-defensin 11 (AvBD11) in the immune response induced by the avian influenza virus H9N2. (2) AvBD11 was expressed using E. coli, and the effects of different concentrations of AvBD11 on cytokine expression in the ex vivo and in vivo erythrocytes of chickens infected with the avian influenza subtype H9N2 were detected by using fluorescence quantification. (3) The results showed that cytokine expression varied among the test groups compared to the control group in the in vitro assay at 2, 6, and 10 h. Lipopolysaccharide induced TNF factor (LITAF) and Interferon-γ (IFN-γ) were significantly increased in the AvBD11 group with the addition of the final concentration of 15 μg/mL at 6 h. At 10 h, Interleukin-1β (IL-1β) and IFN-γ were both more significantly increased in the 15 and 10 μg/mL groups than in the H9N2 group alone. In the in vivo test, IFN-γ and Interleukin-10 (IL-10) were significantly increased in the high-dose group than in the H9N2 group at 3 d and 7 d. (4) In conclusion, the ability of AvBD11 to induce the expression of more cytokines by chicken erythrocytes in a short period of time suggests that it is not only an antimicrobial peptide but also a possible immunomodulator. Full article

The aim of this study was to investigate the expression of immunity-related genes and morpho-histological features of the intestines, and the growth and meat production of broiler chickens when fed plant extracts with different functional components. Chickens in the control group received a basic diet. The feed in the experimental groups contained plant extracts standardized to various biologically active components such as the extract of common chicory with inulin (INUL), St. John’s wort with flavonoids (FLAV), maral root with ecdysterone (ECDS), and extracts of creeping thyme with flavonoids and tannins (FLAV-TANN). The results of this study showed that the application of the studied phytobiotics increased the expression of the pro-inflammatory gene IL8, with the 2^−ΔΔCT value ranging from 2.66 to 4.63. In the case of the antimicrobial peptide gene AvBD9, the 2^−ΔΔCT value ranged from 1.66 to 8.16, depending on the group. AvBD10 gene expression increased (2^−ΔΔCT = 2.19) when the chickens were fed the chicory extract and decreased when thyme extract was used (2^−ΔΔCT = 0.33). The study also found that using biologically active components in the diets of broiler chickens was accompanied by a significant decrease in the height of epithelial cells in the cecum mucous membrane in the INUL group (61.3%, p ≤ 0.001), FLAV group (60.0%, p ≤ 0.001), ECDS group (48.2%, p ≤ 0.001), and FLAV + TANN group (67.6%, p ≤ 0.001). It also caused a reduction in the depth of crypts in the INUL (38.4%, p ≤ 0.001), FLAV (32.3%, p ≤ 0.001), ECDS (50.9%, p ≤ 0.001), and FLAV + TANN (53.4%, p ≤ 0.001) groups. The use of all extracts, except creeping thyme, caused changes in the size of muscular elements in the intestinal walls; thus, the thickness of the muscular layer increased 1.5–2.0 times under the influence of flavonoids in St. John’s wort and of inulin in chicory, to 430.99 and 579.87 μm, respectively (p ≤ 0.001), and decreased 1.9 times under the influence of ecdysterone in maral root to 151.59 μm (p ≤ 0.001). The use of phytobiotics increased feed consumption and stock safety, leading to an increase of 4.1–7.5% in the live weights of broiler chickens at the end of the rearing period, thus contributing to the higher slaughter quality of poultry; in particular, the weights of breast muscles in cockerels in the INUL group increased by 16.9% (p ≤ 0.05), while the weights in the ECDS and FLAV + TANN groups increased by 18.1% (p ≤ 0.05) and 23.1% (p ≤ 0.01), respectively. Thus, the use of the studied phytobiotics in the broiler chicken diet increases meat production and activates immunity, which indicates the possibility of replacing antibacterial drugs with natural nutraceuticals. Full article

Research on the immunological development of lymphoid organs in chicks has been extensive, yet a significant gap exists in our understanding of innate immunity during embryonic life within the intestinal tract. This study investigated the developmental trajectory of intestinal immunity in chick embryos by evaluating basal gene expression levels of key immune markers at embryonic days (ED) 14, 17, and 20. The results indicated variable expression levels of cytokines, antimicrobial peptides (AMPs), and Toll-like receptor (TLRs) genes throughout the intestinal tract. Most cytokines and chemokines exhibited elevated expression in the cecum, while AMPs, including avian-β-defensins (AvBDs) and cathelicidins (CATHs) genes, showed increased levels in the jejunum at ED20. The findings from the developmental trajectory analysis of these genes revealed elevated expression levels of cytokines, including interferon (IFN)-γ, interleukin (IL)-6, IL-13, and transforming-growth factor (TGF)-β in the cecum at ED20. However, no consistent patterns were observed for AvBDs, CATHs, and TLRs, as their expression varied across different developmental stages of the chick embryo. These findings significantly contribute to our understanding of intestinal immune system development in chick embryos and provide a foundation for further research aimed at enhancing immune capabilities, especially in segments with lower expression levels of immunomodulatory genes. Full article

To optimize the outdoor thermal comfort and sunlight hours of elderly residential areas in cold regions of China, we collected data on streets and building forms from 121 elderly residential sites in Beijing. Utilizing parametric modeling tools to generate ideal residential models, a multi-objective optimization algorithm was applied to identify 144 Pareto solutions. The optimal solutions were analyzed using K-means clustering and Pearson correlation analysis to examine how block form affects outdoor environmental performance. The universal thermal climate index (UTCI) in summer showed significant positive correlations (r > 0.72) with the distance between buildings (DB), building density (BD), shape coefficient (SC), and coefficient of variation for building height (CVH), and significant negative correlations (r < −0.82) with average building height (AH), floor area ratio (FAR), volume area ratio (VAR), mean building area (MA), average building volume (AV), and open space ratio (OSR). Winter UTCI was significantly positively correlated with AH, FAR, VAR, MA, and AV (r > 0.83) and significantly negatively correlated with DB, porosity (PO), SC, and CVH (r < −0.88). Sunlight hours were significantly positively correlated with DB, PO, OSR, and CVH (r > 0.84) and significantly negatively correlated with AH, BD, FAR, SC, VAR, MA, and AV (r > 0.88). Courtyard and point-building configurations performed the best across all optimization objectives. (The value of r, Pearson’s correlation coefficient, ranges from −1 to +1. r = +1: Perfect positive correlation, r = −1: Perfect negative correlation, r = 0: No linear correlation). Full article

Over the past few years, there has been substantial interest and research activity surrounding the application of Convolutional Neural Networks (CNNs) for post-filtering in video coding. Most current research efforts have focused on using CNNs with various kernel sizes for post-filtering, primarily concentrating on High-Efficiency Video Coding/H.265 (HEVC) and Versatile Video Coding/H.266 (VVC). This narrow focus has limited the exploration and application of these techniques to other video coding standards such as AV1, developed by the Alliance for Open Media, which offers excellent compression efficiency, reducing bandwidth usage and improving video quality, making it highly attractive for modern streaming and media applications. This paper introduces a novel approach that extends beyond traditional CNN methods by integrating three different self-attention layers into the CNN framework. Applied to the AV1 codec, the proposed method significantly improves video quality by incorporating these distinct self-attention layers. This enhancement demonstrates the potential of self-attention mechanisms to revolutionize post-filtering techniques in video coding beyond the limitations of convolution-based methods. The experimental results show that the proposed network achieves an average BD-rate reduction of 10.40% for the Luma component and 19.22% and 16.52% for the Chroma components compared to the AV1 anchor. Visual quality assessments further validated the effectiveness of our approach, showcasing substantial artifact reduction and detail enhancement in videos. Full article

Over the years, there has been growing interest in multimedia services, especially in the video domain, where firms and subscribers require higher resolutions, framerates, and sampling precision. This results in a huge amount of data that needs to be processed, stored, and transmitted. As a result, researchers face the challenge of developing new compression standards that can reduce the amount of data while maintaining the same quality. In this paper, the compression performance of the latest and most commonly used video codecs, namely H.266/VVC, AV1, H265/HEVC, and H.264/AVC was examined. The test set included seven sequences of various content at 8K, Ultra HD (UHD), and Full HD (FHD) resolutions, encoded to bitrates ranging from 1 to 15 Mbps for FHD and UHD resolutions and from 5 to 50 Mbps for 8K resolution. Objective quality metrics, such as peak signal-to-noise ratio (PSNR), the structural similarity index (SSIM), and video multi-method assessment fusion (VMAF) were used to measure codec performance. The results showed that H.266/VVC outperformed all other codecs, namely H.264/AVC, H.265/HEVC, and AV1, in terms of the Bjøntegaard delta (BD) model. The average bitrate savings were approximately 78% for H.266/VVC, 63% for AV1, and 53% for H.265/HEVC relative to H.264/AVC, 59% for H.266/VVC and 22% for AV1 compared to H.264/AVC, and 46% for H.266/VVC relative to AV1 (all for 8K resolution). The results also showed that codec performance varied depending on resolution, with higher resolutions showing greater efficiency for newly developed codecs, such as H.266/VVC and AV1. This confirms the fact that the H.266/VVC and AV1 codecs were primarily developed for videos at high resolutions, such as 8K and/or UHD. Full article

Fenugreek seeds (FSs) are a natural source of bioactive compounds that may modulate the immune system and gut microbiota in broilers. This study examined the effects of dietary fenugreek seed powder on immune-related gene expression and cecal microbiota composition in broilers. A total of 144 broiler chickens were randomly allocated to three dietary groups, CON (0 g/kg FS, FS5 (5 g/kg FS) and FS10 (10 g/kg FS), each with 6 replicates of 8 birds. Ileum tissues and cecal contents were collected on day 42 for the mRNA expression of inflammation and antimicrobial defense-related genes and cecal microbiome diversity, respectively. The results indicated that fenugreek seeds downregulated mRNA-level inflammation and antimicrobial defense-related genes: IL6, IL8L2, CASP6, PTGS2, IRF7, AvBD9, AvBD10, and AvBD11. Moreover, fenugreek seeds altered the cecal microbial community by increasing the population of Firmicutes and decreasing the population of Actinobacteriota, Gemmatimonadota and Verrucomicrobiota at the phylum level and increasing Alistipes, Bacteriodes and Prevotellaceae at the genera level. These findings suggest that fenugreek seeds have a positive impact on the immunological profile and microbiome of broiler chickens, possibly through the interplay of the immune system and the gut microbiome. Full article

Recent research has been conducted on various types of pre-processing methods for insects, including freeze-drying, microwave drying, hot air heat drying, and non-heat drying. This study aimed to identify the factors that have the greatest impact on heat drying conditions and establish the optimal heat drying conditions for drone pupae (Apis melifera L.) using response surface methodology (RSM) to minimize quality changes. Drone pupae were treated under various conditions, including blanching time (53–187 s) (X₁), drying temperatures (41.6–58.4 °C) (X₂), and drying time (266–434 min) (X₃). The effect of these treatments on response variables, including the color parameter (WI, YI, BI, △E, and BD), AV, and TB of the dried drone pupae, was evaluated using a central composite design. The whole design consisted of 20 experimental points carried out in random order, which included eight factorial points, six center points, and six axial points. The optimal drying conditions for drone pupae were determined to be a blanching time of 58 s, a drying temperature of 56.7 °C, and a drying time of 298 min. The response variables were most affected by drying temperature and drying time and to a lesser extent by blanching time. The processed drone pupae using the optimized drying conditions resulted in the color parameters (WI, BI, YI, ΔE, and BD) being found to be 66.67, 21.33, 26.27, 31.27 and 0.13, respectively. And TB (log CFU/g) and AV (mg/g) values were found to be 3.12 and 4.33, respectively. The estimated and actual values for dried drone pupae showed no significant difference (p < 0.05). Comparing the physicochemical and microbiological properties of freeze-dried and optimal heat-dried drone pupae, the L and b value as well as PV were significantly lower in the heat-dried samples, while no significant difference was observed in the a value and AV (p < 0.05). Our study suggests that the model we developed can be applied to the large-scale production of drying conditions for use in the pharmaceutical and food industries. Full article

In the modern poultry industry, the application of novel phytogenic bioactive compounds with antioxidant potential aims to enhance productivity and quality and to minimize the stress of associated diseases. Herein, myricetin, a natural flavonoid, was evaluated for the first time on broiler chickens’ performance, antioxidants and immune modulating functions, and tackling avian coccidiosis. A total of 500 one-day-old chicks were divided into five groups. The negative (NC) and infected control (IC) groups were fed a control diet without additives, and the latter was infected with Eimeria spp. Groups supplemented with myricetin (Myc) were fed a control diet of Myc (200, 400 and 600 mg/kg diet each). On d 14, all chicks except those in NC were challenged with oocysts of mixed Eimeria spp. Significant improvements in the overall growth rate and feed conversion ratio were detected in the group that was fed 600 mg/kg, unlike the IC group. Notably, groups that were fed 400 and 600 mg/kg showed higher total meat antioxidant capacity with an inverse reduction in oxidative and lipid peroxidation biomarkers (hydrogen peroxide: H₂O₂; reactive oxygen species: ROS; Malondialdehyde: MDA). Of note, the upregulation of glutathione peroxidase; GSH-Px, catalase; CAT, superoxide dismutase; SOD, heme oxygenase-1; HO-1 and NAD(P)H dehydrogenase quinone 1 NQO1 genes in jejunum and muscle were prominently observed with increasing levels of supplemental Myc. At 21 dpi, the severity of coccoidal lesions (p < 0.05) induced by mixed Eimeria spp. and oocyst excretion were greatly reduced in the group that was fed 600 mg/kg of Myc. In the IC group, higher serum levels of C-reactive protein; CRP and nitric oxide; and NO and the upregulated expression of inflammatory biomarkers (interleukin-1β; IL-1β, interleukin-6; IL-6, tumor necrosis factor-α; TNF-α, chemotactic cytokines; CCL20, stromal cell-derived factor-1; CXCL13, and avian defensins; AvBD612) were subsided in higher levels in the Myc-fed groups. Taken together, these findings indicate the promising antioxidant role of Myc in modulating immune responses and reducing growth depression associated with coccidia challenges. Full article

The purpose of the study was to investigate the effects of Eimeria tenella infection on the cecal microbiome, the protein concentration of cecal content, cecal mucosal immunity, and serum endotoxin levels in broilers. Three hundred sixty 14-day-old broilers were allocated to five infection doses with six replicates. The five infection doses were: ID0: 0, ID1: 6250, ID2: 12,500, ID3: 25,000, and ID4: 50,000 Eimeria tenella oocysts. Eimeria tenella infection significantly increased the relative abundance of the phylum Proteobacteria, which includes diverse pathogenic bacteria, and significantly decreased the relative abundance of the phylum Firmicutes. Protein concentration of the cecal content was linearly increased (p < 0.05), and the concentration of secretory immunoglobulin A (sIgA) in the cecal content was linearly decreased by Eimeria tenella infection (p < 0.05). Goblet cell density was linearly reduced in the ceca by Eimeria tenella infection (p < 0.05). Eimeria tenella infection tended to linearly decrease the relative mRNA expression of antimicrobial peptide genes such as avian beta-defensin 9 (AvBD9; p = 0.10) and liver-expressed antimicrobial peptide 2 (LEAP2; p = 0.08) in the cecal tissue. Therefore, Eimeria tenella infection negatively modulated cecal microbiota via impairing cecal mucosal immunity and increasing protein concentration in the cecal content in broilers. Full article

The poor egg-laying performance and short peak egg-laying period restrict the economic benefits of enterprises relating to the Taihe black-bone silky fowl. Ovaries are the main organ for egg production in poultry. Unlike that of mammals, the spawning mechanism of poultry has rarely been reported. As a prominent local breed in China, the reproductive performance of Taihe black-bone silky fowls is in urgent need of development and exploitation. To further explore the egg-laying regulation mechanism in the different periods of Taihe black-bone silky fowls, the ovarian tissues from 12 chickens were randomly selected for transcriptome analysis, and 4 chickens in each of the three periods (i.e., the pre-laying period (102 days old, Pre), peak laying period (203 days old, Peak), and late laying period (394 days old, Late)). A total of 12 gene libraries were constructed, and a total of 9897 differential expression genes (DEGs) were identified from three comparisons; the late vs. peak stage had 509 DEGs, the pre vs. late stage had 5467 DEGs, and the pre vs. peak stage had 3921 DEGs (pre-stage: pre-egg-laying period (102 days old), peak-stage: peak egg-laying period (203 days old), and late-stage: late egg-laying period (394 days old)). In each of the two comparisons, 174, 84, and 2752 differentially co-expressed genes were obtained, respectively, and 43 differentially co-expressed genes were obtained in the three comparisons. Through the analysis of the differential genes, we identified some important genes and pathways that would affect reproductive performance and ovarian development. The differential genes were LPAR3, AvBD1, SMOC1, IGFBP1, ADCY8, GDF9, PTK2B, PGR, and CD44, and the important signaling pathways included proteolysis, extracellular matrices, vascular smooth muscle contraction, the NOD-like receptor signaling pathway and the phagosome. Through the analysis of the FPKM (Fragments Per Kilobase of exon model per Million mapped fragments) values of the genes, we screened three peak egg-laying period-specific expressed genes: IHH, INHA, and CYP19A1. The twelve genes and five signaling pathways mentioned above have rarely been reported in poultry ovary studies, and our study provides a scientific basis for the improvement of the reproductive performance in Taihe black-bone silky fowls. Full article

The effects of glutamine (Gln) on immunity and intestinal barrier gene expression levels in broilers challenged with Salmonella Enteritidis were evaluated. A total of 400 1-day-old broilers were randomly assigned to four groups, 10 repetition treatments per group with 10 broiler chickens for a 21-day feeding trial. The groups were the normal control group (CON, no infected group, fed with a basal diet); the S. Enteritidis-infected control group (SCC, infected with 2.0 × 10⁴ CFU/mL of S. Enteritidis, fed a basal diet); and the Gln 1 and 2 groups, who were challenged with S. Enteritidis and fed a basal diet plus Gln at 0.5% and 1.0%, respectively. The results show that S. Enteritidis had adverse effects on the average daily feed intake, average daily gain, and the feed conversion ratio of infected broilers compared with those of CON broilers on d 7 (p < 0.05); decreased serum immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) concentrations, and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); increased the Lysozyme (LZM, only serum), NO, inducible NO synthase (iNOS) (except at 4 d), and total nitric oxide synthase (TNOS) (except at 4 d) activities in serum and the intestinal mucosa; and increased intestinal mucosa polymeric immunoglobulin receptor (pIgR) (except at 21 d), Avian beta-defensin 5 (AvBD5), AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). Supplementation with Gln improved growth performance; increased serum IgA, IgG, and IgM concentrations and intestinal mucosa Bcl-2 mRNA expression levels (p < 0.05); decreased the LZM (only serum), NO, iNOS (except at 4 d), and TNOS (except at 4 d) activities in serum and the intestinal mucosa; and decreased intestinal mucosa pIgR (except at 21 d), AvBD5, AvBD14, Bax, and Bak mRNA expression levels during the experimental period (p < 0.05). These results suggest that Gln might lessen the inflammatory reaction of the small intestine and enlarge the small bowel mucosa immune and barrier function in broiler chickens challenged with S. Enteritidis. Full article