Search Results (280)

In this paper, we are concerned with the Cauchy problem of the compressible Oldroyd-B model without stress diffusion in ${\mathbb{R}}^n(n=2,3)$. The absence of stress diffusion introduces significant challenges in the analysis of this system. By employing tools from harmonic analysis, particularly the Littlewood–Paley decomposition theory, we establish the global well-posedness of solutions with initial data in $L^p$ critical spaces, which accommodates the case of large, highly oscillating initial velocity. Furthermore, we derive the optimal time decay rates of the solutions by a suitable energy argument. Full article

The cornea, the transparent anterior window of the eye, critically refracts light and protects intraocular structures. Corneal pathologies, including trauma, infection, chemical injury, metabolic diseases, genetic conditions, and age-related degeneration, can lead to significant visual impairment. While penetrating keratoplasty or full-thickness corneal transplantation remains a standard and effective intervention for severe corneal dysfunction, limitations in donor tissue availability and the risk of immunogenic graft rejection necessitate alternative therapeutic strategies. Furthermore, for cases of isolated epithelial disfunction, a full-thickness cornea graft may not be required or effective. This review examines the potential of corneal epithelial constructs derived from autologous stem cells with functional barrier properties for corneal reconstruction and in vitro pharmacotoxicity testing. In this review, we delineate the current limitations of corneal transplantation, the advantages of stem cell-based approaches, and recent advances in generating engineered corneal epithelium. Finally, we address remaining technical challenges and propose future research directions aimed at clinical translation. Full article

The collaborative construction of large-scale, diverse datasets is crucial for developing high-performance machine learning models. However, this collaboration faces significant challenges, including ensuring data security, protecting participant privacy, maintaining high dataset quality, and aligning economic incentives among multiple stakeholders. Effective risk management strategies are essential to systematically identify, assess, and mitigate potential risks associated with data collaboration. This study proposes a federated blockchain-based framework designed to manage multiparty dataset collaborations securely and transparently, explicitly incorporating comprehensive risk management practices. The proposed framework involves six core entities—key distribution center (KDC), researcher (RA), data owner (DO), consortium blockchain, dataset evaluation platform, and the orchestrating model itself—to ensure secure, privacy-preserving and high-quality dataset collaboration. In addition, the framework uses blockchain technology to guarantee the traceability and immutability of data transactions, integrating token-based incentives to encourage data contributors to provide high-quality datasets. To systematically mitigate dataset quality risks, we introduced an innovative categorical dataset quality assessment method leveraging label reordering to robustly evaluate datasets. We validated this quality assessment approach using both publicly available (UCI) and privately constructed datasets. Furthermore, our research implemented the proposed blockchain-based management system within a consortium blockchain infrastructure, benchmarking its performance against existing methods to demonstrate enhanced security, reliability, risk mitigation effectiveness, and incentive alignment in dataset collaboration. Full article

Stipagrostis pennata is an important plant in desert ecosystems. Its seed-endophytic bacteria may play a critical role in plant growth and environmental adaptation processes. This study systematically analyzed the community composition and potential plant growth-promoting (PGP) functions of seed-endophytic bacteria associated with S. pennata. The results showed that while the overall diversity of bacterial communities from different sampling sites was similar, significant differences were observed in specific functional genes and species abundances. Nine endophytic bacterial strains were isolated from the seeds, among which Bacillus altitudinis strain L7 exhibited phosphorus solubilizing capabilities, nitrogen fixing, IAA production, siderophore generation, and multi-hydrolytic enzyme activities. Additionally, the genomic sequencing of L7 revealed the key genes involved in plant growth promotion and environmental adaptation, including Na⁺ efflux systems, K⁺ transport systems, compatible solute synthesis genes, and the gene clusters associated with nitrogen metabolism, IAA synthesis, phosphate solubilization, and siderophore synthesis. Strain L7 exhibits salt and osmotic stress tolerance while promoting plant growth, providing a promising candidate for desert microbial resource utilization and plant biostimulant development. Full article

During a prolonged domestication and environmental selection, Brassica rapa has formed diverse morphological types during a cultivation process of up to 8000 years, such as root-type turnips (Brassica rapa var. rapa), leaf-type Chinese cabbage (Brassica rapa var. pekinensis), oil-type rapeseed (Brassica rapa L.), and other rich types. China is one of the origins of Brassica rapa L., which is spread all over the east, west, south, and north of China. Studying its origin and evolution holds significant importance for unraveling the cultivation history of Chinese oilseed crops, intraspecific evolutionary relationships, and the utilization value of genetic resources. This article summarizes the cultivation history, evolution, classification research progress, and germplasm resource diversity of Brassica rapa var. oleifera in China. Combining karyotype analysis, genomic information, and wild relatives of Brassica rapa var. oleifera discovered on the Qinghai–Tibet Plateau, it is proposed that Brassica rapa var. oleifera has the characteristic of polycentric origin, and Gansu Province in China is one of the earliest regions for its cultivation. Brassica rapa var. oleifera, originating from the Mediterranean region, was diffused to the East Asian continent through two independent transmission paths (one via the Turkish Plateau and the other via Central Asia and Siberia). Analyzing the genetic diversity characteristics and evolutionary trajectories of these two transmission paths lays a foundation for clarifying the origin and evolutionary process of Brassica rapa var. oleifera and accelerating the breeding of Brassica rapa var. oleifera in China. Despite existing research on the origin of Brassica rapa L., the domestication process of this species remains unresolved. Future studies will employ whole-genome resequencing to address this fundamental question. Full article

To explore the dyeing technique and karyotype analysis of winter turnip rape (Brassica rape L.), the root tip of winter turnip rape Longyou 7 was used as the experimental material. Chromosome preparation technology was optimized, and karyotype analysis was carried out by changing the conditions of material collection time, pretreatment, fixation, and dissociation. The results showed that the optimal conditions for the preparation of dyeing winter turnip rape were as follows: the sampling time was 8:00–10:00, the ice–water mixture was pretreated at 4 °C for 20 h, the Carnot’s fixative solution I and 4 °C were fixed for 12 h, and the 1 mol/L HCl solution was bathed in a water bath at 60 °C for 10~15 min. Karyotype analysis showed that the number of chromosomes in winter turnip rape cells was 2n = 20, and the karyotype analysis formula was 2n = 2x = 20 = 16m + 4sm. The karyotype asymmetry coefficient was 58.85%, and the karyotype type belonged to type 2A, which may belong to the primitive type in terms of evolution. The results of this study provide a theoretical basis for further in-depth study of the phylogenetic evolution and genetic trend of Brassica rapa. Full article

The increasing demand for sustainable protein sources has intensified interest in improving the processing efficiency of traditional proteins and developing novel alternatives, particularly those derived from plants and algae. Among various processing technologies, pH shifting has attracted attention due to its simplicity, low cost, and capacity to effectively alter protein structure and functionality. However, employing pH shifting alone requires extremely acidic or alkaline conditions, which can lead to protein denaturation and the generation of undesirable by-products. To address these limitations, this review explores the integration of pH shifting with physical processing techniques such as ultrasound, high-pressure processing, pulsed electric fields, and thermal treatments. Moreover, this review highlights the effects of these combined treatments on protein conformational transitions and the resulting improvements in functional properties such as solubility, emulsification, foaming capacity, and thermal stability. Importantly, they reduce reliance on extreme chemical conditions, providing greater sustainability in industrial applications, particularly in food product development where milder processing conditions help preserve nutritional quality and functional properties. In that sense, this combined treatment approach provides a promising and eco-efficient protein modification strategy, and bridges technological innovation with sustainable resource utilization. Full article

Esophageal squamous cell carcinoma (ESCC) is a prevalent malignancy characterized by poor prognosis and a deficiency of effective therapies. Brusatol (Bru), a bioactive component derived from Brucea javanica, exhibits potent anti-tumor activity. However, the pro-apoptotic and anti-metastatic effects of Bru in ESCC remain unclear. ESCC cells were incubated with Bru. The apoptotic status and metastatic capacities of the cells was measured by the Annexin V-FITC/PI, and wound-healing and transwell assays. Potential targets of Bru in ESCC were identified. The mechanisms by which Bru exerts its effects in ESCC cells were explored. Additionally, the typical 4-NQO-induced ESCC mouse model was employed to examine the anti-tumor effect of Bru in vivo. In this study, Bru was found to trigger mitochondria-mediated cell apoptosis (approximately 5.9- and 3.3-fold increases in the level of apoptosis at high concentrations (80 nM) in the KYSE30 and KYSE450 cells) and inhibit metastasis (49% wound closure decreases at high concentrations (80 nM) in both cells, compared to that in the DMSO group) in ESCC cells. In vivo, Bru significantly suppressed ESCC tumorigenesis. Notably, Bru interacts with Akt1, leading to a reduction in the phosphorylation level of Akt1 at Ser473. Consequently, this not only induced dephosphorylation of Bad at the Ser136 residue to promote mitochondrial apoptosis but also inhibited metastasis in ESCC cells. Bru promoted Bad-mediated mitochondrial apoptosis and inhibited the ESCC cell metastasis by targeting Akt1. Our results suggest Bru is a novel Akt1 inhibitor for inhibiting the progression of ESCC. Full article

We investigated HIV-1 immune evasion mechanisms in a slow progressor (CBJC515) by constructing pseudoviruses expressing autologous Env proteins. Intriguingly, all pseudoviruses exhibited resistance to the broadly neutralizing antibody (bNAb) PGT135. Using site-directed mutagenesis and chimeric Env construction, we identified distinct escape mechanisms: early 2005 strains lost the N332 glycan site, while 2006/2008 strains retained key epitopes but developed resistance through structural modifications in the V1/V4/C2 regions or acquired novel N-glycosylation sites (N398/N611). These findings provide insights into how HIV-1 can escape from N332-directed bNAb responses without altering the epitope itself. Furthermore, chimeric experiments also elucidated regional co-evolution and functional maintenance: the V1V2 region broadly interfered with envelope protein function, while the V3 region may exhibit compensatory activity, restoring functionality and mitigating deleterious polymorphisms in other regions to keep Env antigenic diversity. These results offer valuable mechanistic clues that may inform the development of next-generation HIV-1 vaccines. Full article

This study reveals the damage mechanisms and fracture evolution characteristics of deeply buried granite with prefabricated joints (inclinations of 0°, 30°, 45°, 60°, and 90°) using uniaxial compression tests monitored by Acoustic Emission (AE) technology. Three-dimensional X-CT technology was used to analyze post-damage fracture evolution in specimens with varying joint inclinations. The results show that the stress–strain curve of deeply buried jointed granite under uniaxial compression includes three stages: initial compaction, crack extension, and failure. AE characteristics align with these stages, showing clear stress responses and timing features. In the initial compaction stage, micro-crack closure dominates, with smaller joint inclinations showing stronger closure effects. In the crack extension stage, joint inclination determines the crack propagation mode. In the failure stage, joint inclination significantly affects the spatial distribution of the rupture network by altering stress concentration areas and crack types. The proportion of shear micro-cracks increases with joint inclination, and peak strength rises with increasing joint angle, potentially accelerating micro-crack evolution. These findings provide valuable insights for designing excavation and instability monitoring in deeply buried multi-jointed granite underground projects. Full article

Conventional metalenses struggle with chromatic aberration and narrow field of view (FOV), making it challenging to meet the dispersion requirements for large apertures and compensate off-axis aberrations for wide FOV. Here, we demonstrate a hybrid metalens module consisting of five refractive plastic lenses and a polarization-insensitive metalens to achieve broadband achromatic imaging within 400–700 nm and a wide FOV up to 100°. The system exhibits negligible variation in focal length (~1.2%) across the visible range (460–656 nm) and consistently achieves modulation transfer function (MTF) values > 0.2 at 167 lp/mm across all wavelengths and incident angles. We also demonstrate integrated lens modules that capture high-quality images from distances ranging between 0.5 and 4 m without post-processing, showcasing its potential for compact, wide-angle optical systems. Full article

Sclerostin, encoded by the SOST gene, is a novel bone anabolic target for bone diseases. Humanized anti-sclerostin antibody, romosozumab, was approved for treatment of postmenopausal osteoporosis by the US Food and Drug Administration (FDA), but with a black-box warning on cardiovascular risk. The clinical data regarding cardiovascular events from various pre-marketing and post-marketing studies of romosozumab were inconsistent. Overall, the cardiovascular risk of sclerostin inhibition could not be excluded. The restriction of romosozumab in patients with cardiovascular disease history would be necessary. Moreover, genome-wide association study (GWAS) analyses of SOST variants revealed inconsistent results of the association between SOST variations and cardiovascular diseases. Further research incorporating larger sample sizes and functional analyses are necessary. In analyses of serum/tissue sclerostin levels in patients with cardiovascular diseases, the results were controversial but indicated an association between sclerostin and the presence/severity/outcomes of cardiovascular diseases. Nonclinical studies in rodents indicated the inhibitory effect of sclerostin on inflammation, aortic aneurysm, atherosclerosis, and vascular calcification. Sclerostin loop3 participated in the inhibitory effect of sclerostin on bone formation, while the cardiovascular protective effect of sclerostin was independent of sclerostin loop3. Macrophagic sclerostin loop2–apolipoprotein E receptor 2 (ApoER2) interaction participated in the inhibitory effect of sclerostin on inflammation in vitro. Sclerostin in human aortic smooth muscle cells participated in the reduction in calcium deposition. The role of sclerostin in cardiovascular system deserves further investigation. Full article

Eutrophication challenges aquatic ecosystems, with wetland plants serving as sustainable sources of in situ remediation for nitrogen (N) and phosphorus (P) removal. The limited understanding of seasonal nutrient dynamics hinders sustainable management development. This review classifies wetland plants by growth patterns: spring–summer growth plants (SSPs), spring–summer–autumn growth plants (SSAPs), and all-year-round growth plants (APs). SSPs exhibit peak N/P in spring–summer, SSAPs sustain high levels in autumn, while APs maintain stable contents through cold-tolerant sustainability. Perennials achieve sustainable cycling by seasonally storing N/P in tissues and redistributing them in spring. We propose the following sustainable harvesting protocols: harvest SSPs pre-September, SSAPs by November, and retain APs over winter. Height-controlled harvesting during growth peaks enhances removal while preserving regeneration. Strategic combinations of SSPs, SSAPs, and APs ensure sustainable, efficient nutrient removal across hydrological/climatic conditions, supporting wetland conservation. Full article

Ensuring the profitability of retailers utilizing in-store inventory for online fulfillment is a pivotal issue in omnichannel retailing. This study examines the inventory allocation challenges faced by retailers when managing interactions between online and offline channels to identify strategies that maximize revenue. The findings enable retailers to address key operational conflicts while implementing omnichannel strategies. We develop an omnichannel newsvendor model, deriving an optimal strategy for retailer inventory level and online acceptance thresholds, demonstrating the economic superiority of this approach over traditional policy. Furthermore, this paper further explores how carry-over inventory influences strategic decisions, particularly in quantifying the trade-off between the cancellation cost and the inventory holding cost. The results reveal that cancellation costs incentivize retailers to increase safety stock and reduce online acceptance thresholds, with strategy sensitivity intensifying as offline demand dispersion grows. Compared to the traditional policy, our policy demonstrates superior performance when the cancellation cost remains below a critical value, though its effectiveness decreases under high offline demand dispersion. Moreover, dynamic strategy adjustments must balance the cancellation cost against the holding cost in the carry-over scenario. The proposed framework systematically integrates inventory allocation with demand admission control, addressing a critical gap in existing literature that has failed to comprehensively link these two operational levers. This dual-focused perspective significantly advances omnichannel inventory management theory. Full article

The CPA gene family regulates ionic balance and pH homeostasis in cells, significantly contributing to plant stress tolerance. In this study, a total of 63 BrCPA gene family members were identified in the whole genome of Brassica rapa L. (B. rapa), and the three subfamily members were BrNHX (9), BrKEA (15), and BrCHX (39), respectively. The members of the BrCPA gene family encoded 303-1259 amino acids, with molecular weights in the range of 32,860.39~139,884.73 kDa, distributed on 10 chromosomes, and contained 17 conserved motifs, BrNHX and BraKEA, and the BrCPA gene family members had the same molecular weights on 10 chromosomes and contain 17 conserved motifs. The BrNHX and BraKEA subfamilies have more exons than the BrCHX subfamily. An analysis of promoter cis-acting elements in the BrCPA gene showed that members of this gene family contain TC-rich, LTR, MBS, and ARE stress response elements. In addition, transcriptome analysis revealed the expression of CPA genes in B. rapa under salt stress. The selected genes were verified by RT-qPCR. By detecting the Na⁺ and K⁺ flow rates in the root and chloroplast cells of salt-tolerant and salt-sensitive varieties after salt treatment, it was found that the rate of Na⁺ and K⁺ efflux from the root and chloroplast cells of salt-sensitive varieties was significantly higher than that of salt-tolerant varieties. This investigation marks the first systematic identification of the CPA gene family in B. rapa. This study further explores its expression patterns and the efflux rates of Na⁺ and K⁺ across salt-tolerant varieties, providing a theoretical basis for understanding the role of the CPA gene family in the salt stress response of B. rapa. Full article