Search Results (421)

Background and Objectives: Depression is a leading cause of disability globally, with treatment challenges including limited access, stigma, and poor adherence. Gamification, which applies game elements such as points, levels, and storytelling into non-game contexts, offers a promising strategy to enhance engagement and augment traditional treatments. Our research is the first study designed to explore the implementation of gamification within the Malaysian context. The objective was to explore the feasibility of implementation of gamification as an adjunctive treatment for adults with depression. Materials and Methods: Focus group discussions were held with five mental health professionals and ten patients diagnosed with moderate depression. The qualitative component assessed perceptions of gamified interventions, while quantitative measures evaluated participants’ depressive and anxiety symptomatology. Results: Three key themes were identified: (1) understanding of gamification as a treatment option, (2) factors influencing its acceptance, and (3) characteristics of a practical and feasible intervention. Clinicians saw potential in gamification to boost motivation, support psychoeducation, and encourage self-paced learning, but they expressed concerns about possible addiction, stigma, and the complexity of gameplay for some patients. Patients spoke of gaming as a source of comfort, escapism, and social connection. Acceptance was shaped by engaging storylines, intuitive design, balanced difficulty, therapist guidance, and clear safety measures. Both groups agreed that gamification should be used in conjunction with standard treatments, be culturally sensitive, and be presented as a meaningful therapeutic approach rather than merely as entertainment. Conclusions: Gamification emerges as an acceptable and feasible supplementary approach for managing depression in Malaysia. Its success depends on culturally sensitive design, robust clinical oversight, and seamless integration with existing care pathways. Future studies should investigate long-term outcomes and establish guidelines for the safe and effective implementation of this approach. We recommend targeted investment into culturally adapted gamified tools, including training, policy development, and collaboration with key stakeholders to realistically implement gamification as a mental health intervention in Malaysia. Full article

Mycoplasma bovis is an important pathogen that is associated with respiratory diseases, mastitis, and arthritis in cattle, leading to significant economic losses in the global cattle industry. Most notably in this study, we pioneer the discovery that its secreted effector ENO1 (α-enolase) directly targets host cytoskeletal proteins for metabolic–immune regulation. Using an innovative GST pull-down/mass spectrometry approach, we made the seminal discovery of β-actin (ACTB) as the primary host target of ENO1—the first reported bacterial effector–cytoskeleton interaction mediating metabolic reprogramming. ENO1–ACTB binding depends on a hydrogen bond network involving ACTB’s 117Glu and 372Arg residues. This interaction triggers (1) glycolytic activation via Glut1 upregulation, establishing Warburg effect characteristics (lactic acid accumulation/ATP inhibition), and (2) ROS-mediated activation of dual inflammatory axes (HIF-1α/IL-1β and IL-6/TNF-α). This work establishes three groundbreaking concepts: (1) the first evidence of a pathogen effector hijacking host ACTB for metabolic manipulation, (2) a novel ‘glycolysis–ACTB–ROS-inflammation’ axis, and (3) the first demonstration of bacterial proteins coordinating a Warburg effect with cytokine storms. These findings provide new targets for anti-infection therapies against Mycoplasma bovis. Full article

Background/Objectives: Advancements in DNA profiling have made it possible to retrieve intact DNA profiles from increasingly minute biological samples. This increased sensitivity in DNA detection has highlighted crucial considerations to be made when handling and packing items from the crime scene to minimize potential contamination from either direct or indirect transfer of DNA. To investigate potential DNA transfer between items stored within the same evidence package, we conducted a simulation study with items commonly encountered during forensic casework. Methods: Participants were grouped in pairs, each of them handling the same type of item to simulate the activity conducted at the crime scene. The items were then collected from each pair of participants and stored in the same evidence package for 4 to 5 days. To evaluate the basal DNA transfer between items within the same package, the packed items were not subjected to friction, force, or long-distance movement in this study. Results: We have observed the occurrence of DNA transfer on 39% of the studied items inside the package, which changed the source attribution of the DNA profiles for 10% of the recovered samples. Our results showed that the types of items were associated with the number of transferred alleles and the amount of DNA recovered, while no association was found between the number of transferred alleles and the amount of DNA on the studied items. Conclusions: Taken together, the results from this study reiterate the importance of packing each item from the crime scene separately, especially when packing items together may impact the interpretation of source attribution. Full article

As semiconductor devices demand higher input–output density and faster signal transmission, fan-out panel-level packaging has emerged as a promising solution for next-generation electronic systems. However, the hygroscopic nature of epoxy molding compounds raises critical reliability concerns under high-temperature and high-humidity conditions. This study investigates the hygrothermal stress of a single fan-out panel-level package unit through experimental characterization and numerical simulation. Thermal–mechanical analysis was conducted at 100 °C and 260 °C to evaluate the strain behavior of two commercial epoxy molding compounds in granule form after moisture saturation. The coefficient of moisture expansion was calculated by correlating strain variation with moisture uptake obtained under 85 °C and 85% relative humidity, corresponding to moisture sensitivity level 1 conditions. These values were directly considered into a moisture -thermal coupled finite element analysis. The simulation results under reflow conditions demonstrate accurate principal stress and failure location predictions, with stress concentrations primarily observed at the die corners. The results confirm that thermal effects influence stress development more than moisture effects. Finally, a structural sensitivity analysis of the single-package configuration showed that optimizing the thickness of the dies and epoxy molding compound can reduce maximum principal stress by up to 12.4%, providing design insights for improving package-level reliability. Full article

The blue-eared pheasant (Crossoptilon auritum), a Near Threatened (NT) species endemic to China, is primarily distributed across the northeastern region of the Qinghai–Tibetan Plateau. To bridge the fine-scale spatiotemporal gap in blue-eared pheasant behavioral ecology, this study combines satellite telemetry, movement modeling, and field-based habitat assessments (vegetation, topography, human disturbance). This multidisciplinary approach reveals detailed patterns of their behavior throughout the breeding season. Using satellite-tracking data from six individuals (five males tracked at 4 h intervals; one female tracked hourly) in Wanglang National Nature Reserve (WLNNR), Sichuan Province during breeding seasons 2018–2019, we quantified their home ranges via Kernel Density Estimation (KDE) and examined the female movement patterns using a Hidden Markov Model (HMM). The results indicated male core (50% KDE: 21.93 ± 16.54 ha) and total (95% KDE: 158.30 ± 109.30 ha) home ranges, with spatial overlap among individuals but no significant temporal variation in home range size. Habitat selection analysis indicated that the blue-eared pheasants favored shrub-dominated areas at higher elevations (steep southeast-facing slopes), regions distant from human disturbance, and with abundant animal trails. We found that their movement patterns differed between sexes: the males exhibited higher daytime activity yet slower movement speeds, while the female remained predominantly near nests, making brief excursions before returning promptly. These results enhance our understanding of the movement ecology of blue-eared pheasants by revealing fine-scale breeding-season behaviors and habitat preferences through satellite-tracking. Such detailed insights provide an essential foundation for developing targeted conservation strategies, particularly regarding effective habitat management and zoning of human activities within the species’ range. Full article

The internal growth patterns and surface micromorphology of diamonds provide a record of their multi-stage evolution, from initial formation within the mantle to their eventual ascent to the Earth’s surface via deeply derived kimberlite magmas. In this study, gemological microscopic examination, Diamond View^TM, Raman spectroscopy, and electron probe analysis were employed to analyze the surface features, internal patterns, and inclusions of the Tancheng alluvial diamonds in Shandong Province, China. The results show that surface features of octahedra with triangular and sharp edges, thick steps with irregular contours or rounded edges, and thin triangular or serrated layers are developed on diamonds during deep-mantle storage, as well as during the growth process of diamonds, when they are not subjected to intense dissolution. The rounding of octahedral and cubic diamond edges and their transformation into tetrahedral (THH) shapes are attributed to resorption in kimberlitic magma. These characteristics indicate that the Tancheng diamonds were commonly resorbed by carbonate–silicate melts during mantle storage. Abnormal birefringence phenomena, including irregular extinction patterns, petaloid and radial extinction patterns, and banded birefringence, were formed during the diamond growth stage. In contrast, fine grid extinction patterns and composite superimposed extinction patterns are related to later plastic deformation. The studied diamonds mainly contain P-type inclusions of olivine and graphite, with a minority of E-type inclusions, including coesite and omphacite. The pressure of entrapment of olivine inclusions within the Tancheng diamonds ranges from 4.3 to 5.9 GPa, which is consistent with that of coesite inclusions, which yield pressure ranging from 5.2 to 5.5 GPa, and a temperature range of 1083–1264 °C. Overall, the evidence suggests that Tancheng diamonds probably originated from hybrid mantle sources metasomatized by the subduction of ancient oceanic lithosphere. Full article

A series of fire experiments were conducted in a 0.5 m × 0.5 m × 0.5 m room, and a single door-like opening was adopted. The height of the openings was 20 cm, and the width of the openings varied from 10 cm to 30 cm, with ventilation factors ranging from 0.0089 m^5/2 to 0.0268 m^5/2. The ventilation constant and combustion efficiency were studied and compared with those of other researchers. It was found that the so-called ventilation constant can hardly be a constant, and it varied greatly, around 0.357–0.436, at different ventilation conditions. The overall combustion efficiency varied greatly at different opening sizes and flow rates, and it was as low as 0.5, even when the flame was ejected. Full article

Natural and planted forests differentially regulate soil quality through vegetation–soil interactions. The effects of four types of planting covers on soil nutrients, enzyme activities, and microbial communities in the Guangxi Camellia nitidissima National Nature Reserve were studied, revealing the multi-dimensional influences of natural (broadleaf, shrubland) and planted forests (bamboo, pine) on soil quality. Surface soils (0–10 cm depth) were characterized for physicochemical properties (pH, TC, TN, NO₃⁻-N, AP), enzyme activities (α-amylase, urease, phosphatase, β-glucosidase), and microbial composition (using 16S rRNA and ITS region sequencing). Mantel tests and PLS–PM modeling were employed to investigate interactions among vegetation, soil variables, and microbes. Natural forests exhibited higher pH, nitrate nitrogen, and enzymatic activities (urease, phosphatase, β-glucosidase) alongside enhanced carbon–nitrogen accumulation and reduced acidification. Planted forests showed elevated available phosphorus and nutrient supply but lower organic matter retention. Microbial communities displayed higher similarity within natural forests, with fungal composition strongly linked to total carbon/nitrogen (p < 0.05). Vegetation type positively influenced bacterial diversity but negatively affected fungal communities. Natural forests maintained critical soil–microbe–plant interactions supporting ecosystem resilience through carbon–nitrogen cycling, while planted forests fostered divergent microbial functionality despite short-term nutrient benefits. These findings underscore natural forests’ unique role in preserving ecological stability and reveal fundamental limitations of artificial systems in mimicking microbially-mediated biogeochemical processes. Conservation policy should prioritize the protection of natural forests while simultaneously integrating microbial community management with vegetation restoration efforts to enhance long-term ecosystem functionality and nutrient cycling efficiency. Full article

Copper complexes have attracted significant interest for catalyzing oxidative dehydrogenative carboxylation of alkanes to form esters. Here, we report a heterogeneous catalyst, in which copper complexes are immobilized on coal-bearing kaolin for the synthesis of allylic esters via C(sp³)-H bond activation through cross-dehydrogenation coupling reactions between cyclic alkanes and aromatic carboxylic acids. Systematic optimization of reaction conditions—including catalyst loading, copper content, oxidant, temperature, and reaction time—resulted in a high yield of 71% of allylic ester, comparable to homogeneous transition metal catalysts. The catalyst is easily recoverable via centrifugation and retains its activity over five consecutive reuse cycles. This system demonstrates broad substrate compatibility with various aromatic carboxylic acids and cyclic alkanes. Beyond offering an efficient and reusable catalytic route for allylic ester synthesis, this work highlights the potential of coal-bearing kaolin as a sustainable support material for transition metal catalysis and provides an environmentally benign method for activating inert C(sp³)–H bonds. Full article

With the increasing scarcity of traditional hardwood sawdust resources, developing sustainable substrates for edible fungi cultivation has become an urgent industrial priority. This study systematically evaluated the effects of bamboo sawdust substitutions (20%, 30%, 40%, and 50%) on mycelial growth, fruiting body development, and nutritional quality of Auricularia heimuer, while elucidating the underlying molecular mechanisms through transcriptome sequencing. The results demonstrated that bamboo substitution of ≤30% maintained normal mycelial growth and fruiting body differentiation, with 20% and 30% substitutions increasing yields by 5.30% and 3.70%, respectively, compared to the control. However, 50% substitution significantly reduced yield by 9.49%. Nutritional analysis revealed that 20–40% bamboo substitution significantly enhanced the contents of crude protein, polysaccharides, and essential minerals (calcium, iron, and selenium) in fruiting bodies. Transcriptome analysis identified upregulation of glycosyl hydrolase family genes and downregulation of redox-related genes with increasing bamboo proportions. Biochemical assays confirmed these findings, showing decreased oxidative substances and increased reductive compounds in mycelia grown with high bamboo content, which indicate disrupted cellular redox homeostasis. This study provides both a practical solution to alleviate the “edible mushrooms derived from lignicolous fungi–forest conflict” and fundamental insights into fungal adaptation mechanisms to non-wood substrates, thus establishing a theoretical foundation for the valorization of agricultural and forestry wastes. Full article

Sugarcane (Saccharum officinarum L.) faces significant challenges in China, including labor-intensive cultivation, low yields, and environmental stresses. Enhancing root development and stress tolerance through phytohormones and molecular breeding is a promising approach to boosting productivity. Indole-3-butyric acid is a phytohormone known for promoting root development and stress resistance. However, its effects on sugarcane root development under low temperature remain poorly understood. This study demonstrated that IBA markedly promoted root initiation, elongation, and biomass under low temperature, and significantly increased the levels of phytohormones, including GA₃, ABA, JA, IAA, and ZT, suggesting the activation of multiple signaling pathways. Transcriptome analysis revealed numerous differentially expressed genes related to metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and glutathione metabolism. Weighted gene co-expression network analysis identified core gene modules correlated with phytohormone activities, highlighting their role in the IBA-mediated stress response. Eleven core genes, including GSTU6, FAR1, and BCAT3, and nine hub genes, such as Ub-CEP52-1 and ACS1, were identified as critical components for IBA-induced root development and stress mitigation. These findings provide insights into the molecular mechanisms underlying IBA-induced root development and stress tolerance in sugarcane, offering candidate genes for breeding high-yield, stress-tolerant varieties and demonstrating IBA’s potential as a strategy to enhance productivity under challenging conditions. Full article

In this study, a novel lignin-based magnetic composite with a shell-and-core structure and high saturated magnetic strength has been developed for the efficient removal of Pb(II) from wastewater. The adsorbent was fabricated through the introduction of silica–amino groups and a cross-linking complex with lignin, utilizing Fe-Fe₂O₃ as a magnetic source. The paramagnetic characteristics enabled its rapid separation from the aqueous solution within merely 15 s. Batch adsorption experiments demonstrated that the adsorbents could reach equilibrium for Pb(II) adsorption within 30 min. When the concentration of Pb(II) is in the low range of 0 to 200 mg/L, the removal rate of Pb(II) approaches 100%, and the theoretical maximum adsorption capacity is as high as 384.2 mg/g. The mechanism analysis indicated that the adsorption process was primarily characterized as monolayer chemisorption. Notably, the resultant bio-composites demonstrated a high level of stability even after eight consecutive adsorption and desorption cycles, with the removal rate of Pb(II) still reaching 82.3%. This work outlines a novel approach for designing highly efficient lignin-derived adsorbents toward wastewater treatment. Full article

Acute erythroid leukemia (AEL) is a rare and aggressive hematological malignancy managed with chemotherapy, targeted therapies, and stem cell transplantation. However, these treatments often suffer from limitations such as refractoriness, high toxicity, recurrence, and drug resistance, underscoring the urgent need for novel therapeutic approaches. Dibromo-edaravone (D-EDA) is a synthetic derivative of edaravone (EDA) with unreported anti-leukemic properties. In this study, D-EDA demonstrated potent cytotoxicity against HEL cells with an IC₅₀ value of 8.17 ± 0.43 μM using an MTT assay. Morphological analysis via inverted microscopy revealed reductions in cell number and signs of cellular crumpling and fragmentation. Flow cytometry analysis, Hoechst 33258 staining, Giemsa staining, a JC-1 assay, and a reactive oxygen species (ROS) assay showed that D-EDA induced apoptosis in HEL cells. Furthermore, D-EDA induced S-phase cell cycle arrest. Western blot analysis showed significant upregulation of key apoptosis-related proteins, including cleaved caspase-9, cleaved caspase-3, and cleaved poly ADP-ribose polymerase (PARP), alongside a reduction in Bcl-2 expression. Additionally, oncogenic markers such as c-Myc, CyclinA2, and CDK2 were downregulated, while the cell cycle inhibitor p21 was upregulated. Mechanistic studies involving molecular docking, a cellular thermal shift assay (CETSA), the caspase inhibitor Z-VAD-FMK, JAK2 inhibitor Ruxolitinib, and STAT3 inhibitor Stattic revealed that D-EDA activates the caspase cascade and inhibits the JAK2-STAT3 signaling pathway in HEL cells. In vivo, D-EDA improved spleen structure, increased the hemolysis ratio, and extended survival in a mouse model of acute erythroleukemia. In conclusion, D-EDA induces apoptosis via the caspase cascade and JAK2-STAT3 signaling pathway, demonstrating significant anti-leukemia effects in vitro and in vivo. Thus, D-EDA may be developed as a potential therapeutic agent for acute erythroleukemia. Full article

The Trusted Execution Environment (TEE) is crucial for safeguarding the ecosystem of embedded systems. It uses isolation to minimize the TCB (Trusted Computing Base) and protect sensitive software. It is vital because devices handle vast, potentially sensitive data. Leveraging ARM TrustZone, widely used in mobile and IoT for TEEs, it ensures hardware protection via security extensions, though needing firmware and software stack support. Despite the reputation of TEEs for high security, TrustZone-aided ones have vulnerabilities. Fuzzing, as a practical bug-finding technique, has seen limited research in the context of TEE. The unique software architecture of TrustZone-assisted TEE complicates the direct application of traditional fuzzing methods. Moreover, simplistic approaches, such as feeding random input values into TEE through the API functions of the rich operating system, fail to uncover deeper, latent bugs within the TEE code. In this paper, we present a fuzzing strategy for TrustZone-assisted TEE that utilizes inferred dependencies between Trusted Kernel system calls to uncover deep-seated TEE bugs. We implemented our approach on OP-TEE, where it successfully identified 17 crashes, including one previously undetected kernel bug. Full article

To address the challenge of recognizing sheep faces of the same type, which exhibit significant similarities and varying performance of RGB images under different lighting conditions and angles, this paper proposes a dual-branch multi-modal sheep face recognition model based on the ResNet18 architecture. This model effectively learns geometric features from depth data and texture features from RGB data, thereby enhancing recognition accuracy. Initially, the model employs two InceptionV2 layers, one for the RGB channel and another for the depth channel, to extract specific features from both modalities. Subsequently, the losses from the two modalities are computed. In the mid-stage, the two modalities are fused using the Convolutional Block Attention Module (CBAM), and in the final stage, a residual network is utilized to learn the complementary features between the modalities. Experimental results demonstrate that this model benefits from effective multi-modal fusion, achieving high accuracy in sheep face recognition under complex lighting conditions and various angles. Full article