Search Results (27)

In recent years, outbreaks of Seneca Valley virus (SVV) in pig farms have raised concerns about disease resistance in different pig breeds. Min pigs are an excellent local pig breed in China, but the breed’s strong disease resistance mechanism has not been clearly investigated. In this study, Min pigs and Landrace pigs were challenged with SVV, and the differences in pathogenicity between SVV-infected Min and Landrace pigs were evaluated in terms of production performance, survival rate, immune cell activity, pathological changes, viral titer, and cytokine expression. The results show that the mortality rate in Min pigs was significantly lower than that in Landrace pigs without substantial weight loss. The copy number of SVV RNA in the intestinal mucosa of Min pigs was lower than that in Landrace pigs. Additionally, the IgA and IgG titers in Min pigs were higher than those in Landrace pigs. Both the absolute number and percentage of M1 macrophages were elevated in Min pigs relative to Landrace pigs. This study is the first to identify differences in macrophage activity between SVV-infected Min pigs and Landrace pigs. The presented results indicate the potential research value of studying innate immunity differences in disease resistance between pig breeds. Full article

Composite overwrapped pressure vessels (COPVs) have become the core unit for high-pressure hydrogen storage and transportation. However, excessive autofrettage pressure could induce unilateral buckling damage of the metal liner because of large rebound compressive stress induced by large plastic deformation in the depressurization stage. When the liner contains initial defects, its critical unilateral buckling pressure would be further reduced. In this paper, a critical buckling pressure calculation formula was established by finite element analysis and theoretical derivation. Firstly, the classical theoretical calculation models and research methods were analyzed and discussed. Then, by discussing the key influencing parameters, a semi-empirical calculation formula of nonlinear confined buckling pressure of a metal liner with ovality defects was established. Finally, the proposed semi-empirical formula was used to predict the critical internal pressure of a Type-III COPV, and the predicted value was compared with the experimental result. The predicted result was higher than the experimental result and the error range was −2.8%~−23%. The proposed semi-empirical formula of nonlinear confined buckling could provide theoretical support for designing the autofrettage pressure of Type-III COPVs and help to reduce the uncertainty and repeated test cost in the design process. Full article

Climate change is recognized to directly and indirectly affect groundwater systems. However, the mechanisms through which climate change influences groundwater temperature (GWT), particularly how seasonal variations mediate these effects, remain incompletely understood. This study utilized high-temporal-resolution (hourly) data by parameterizing groundwater levels (GWLs) and instantaneous temperature gradients to model GWT, establishing the Seasonally Adaptive Thermal Diffusivity Numerical Model (SATDNM). Through scenario analyses, the potential impacts of climate change on GWT were simulated. The results indicate that our model captures seasonal and interannual variations more precisely compared to classical models, revealing the seasonal influence of GWLs and instantaneous temperature gradients on subsurface thermal properties such as advents and wet-season rainfall, as well as long-term surface warming and GWL decline. The key findings include (1) a greater sensitivity to extreme heat during winter, (2) wet-season rainfall potentially stabilizing groundwater temperature, and (3) declining GWLs amplifying GWT fluctuations. By 2100, the projected mean GWT increases under four Shared Socioeconomic Pathway (SSP) scenarios are approximately 0.51 °C (SSP1-2.6), 1.25 °C (SSP2-4.5), 2.19 °C (SSP3-7.0), and 2.87 °C (SSP5-8.5). Under four scenarios of annual GWL decline rates, GWT fluctuations increased by approximately 0.094 °C (0.01 m/year), 0.27 °C (0.02 m/year), 0.44 °C (0.03 m/year), and 0.67 °C (0.04 m/year), respectively. These findings enhance the mechanistic understanding of climate–groundwater thermal interactions and provide new insights for adaptive groundwater management under climate change. Full article

This paper introduces a new self-locking-unlockable modular building with an inter-module connection, and its seismic performance is investigated. The new connection can realize fast connection and unlocking during construction through exceptional design. In this paper, taking the Tianjin Binhai Apartment project as the background, for the actual force situation of the new connection, considering the influence of corrugated steel plate stiffness, a simplified model of the connection is constructed by using multi-fold elastic connection, and the corrugated steel plate stiffness is simulated with equivalent support. In the MIDAS Gen 2021 software, the five-story and six-story structural models using traditional rigid connections and new connections were established, respectively, and reaction spectrum analysis was carried out. Meanwhile, seismic waves that comply with codes were selected for dynamic time course analysis. The results show that the stress ratios of all components of the new connection model and the traditional rigid model are less than 1. Among them, the maximum stress ratios of both floor beams are 0.745 and 0.725, respectively; the maximum stress ratios of the modular columns are 0.655 and 0.494, respectively; the stress ratios of the ceiling beams are all less than 0.5; and the two models show good strength and stiffness reserves, following the design principle of strong columns and weak beams and verifying the reliability of the new connection model. Meanwhile, it is found that the inter-story displacement angle of the six-story structure with the new connections is less than the normative value under the action of rare earthquakes, and the difference in top displacement is about 18% compared with that of the rigid structure, so it is suggested that the new connections can be applied within the height of six stories. Full article

Three pairs of enantiomers (1–3)—the new 12R-aloesol (1a) and two new fatty acids (2 and 3)—and one new natural product (4) together three known compounds (5–7) were isolated from a coral-reef-derived Streptomyces sp. SCSIO 66814. Their structures were determined through extensive spectroscopic analysis, chiral analysis, and single-crystal X-ray diffraction data. Compounds 2 and 3 were presumed to be intermediates for further generating homononactic acid (5) and nonactic acid, and the latter two molecules were able to act as precursors to form macrotetrolides with remarkable biological activity. The isolation of related precursors, compounds 2–5, provided more evidence to support the proposal of a plausible biosynthetic pathway for nonactic acid and its homologs. Additionally, (+)-1 exhibited a weak activity against DPPH radicals. Full article

An ethyl acetate extract of a marine actinomycete strain, Nocardiopsis mentallicus SCSIO 53858, isolated from a deep-sea sediment sample in the South China Sea, exhibited anti-quorum-sensing (QS) activity against Chromobacterium violaceum CV026. Guided by the anti-QS activity, a novel active compound was isolated and purified from the extract and was identified as 2,3-dimethoxycinnamic acid (2,3-DCA) through spectral data analysis. At a concentration of 150 μg/mL, 2,3-DCA exhibited robust inhibitory effects on three QS-regulated traits of C. violaceum CV026: violacein production, swarming motility, and biofilm formation, with inhibition rates of 73.9%, 65.9%, and 37.8%, respectively. The quantitative reverse transcription polymerase chain reaction results indicated that 2,3-DCA can disrupt the QS system in C. violaceum CV026 by effectively suppressing the expression of QS-related genes, including cviR, vioA, vioB, and vioE. Molecular docking analysis revealed that 2,3-DCA hinders the QS system by competitively binding to the same binding pocket on the CviR receptor as the natural signal molecule N-hexanoyl-L-homoserine lactone. Collectively, these findings suggest that 2,3-DCA exhibits promising potential as an inhibitor of QS systems, providing a potential solution to the emerging problem of bacterial resistance. Full article

Patching is a common technology used in repairing asphalt-pavement potholes. Due to the differences in material properties between patched- and unpatched-asphalt mixtures, significant strain and stress concentrations could be induced; thus, further cracks and interfacial debonding distress could be caused. As a remedy, the strain and stress concentrations can be alleviated by utilizing optimum patching shapes. Therefore, this paper employed finite element methods (FEM) to deeply analyze the mechanical performance of patched-asphalt pavements embedded with different patching shapes. Three patching shapes, these being rectangular, stair, and trapezoid, were considered for use in pavement pothole repairs based on two- and three-dimensional finite element models. In the two-dimensional models, Top-Down and Bottom-Up crack propagations were simulated to assess the anti-damage performance of the patched pavements with different patching shapes. In addition, the thermal stress behaviors within patched-asphalt pavements were simulated using the two-dimensional model to analyze the performance of the patched pavements during the cooling process in construction. In addition, interface-debonding performance was simulated for the patched-asphalt pavements using three-dimensional models. In light of the simulation results, engineers are expected to better understand the mechanism within patched pavements and to improve the quality of the pavement patching. Full article

Bioconversion of lignocellulosic biomass is a highly promising alternative to rapidly reduce reliance on fossil fuels and greenhouse gas emissions. However, the use of lignocellulosic biomass is limited by the challenges of efficient degradation strategies. Given this need, Bacillus tropicus (B. tropicus) with cellulose degradation ability was isolated and screened from rotten dahlia. The strain efficiently utilized coconut oil cake (COC) to secrete 167.3 U/mL of cellulase activity. Electron microscopy results showed significant changes in the structure and properties of cellulose after treatment with B. tropicus, which increased the surface accessibility and the efficiency of the hydrolysis process. The functional group modification observed by Fourier transform infrared spectroscopy indicated the successful depolymerization of COC. The X-ray diffraction pattern showed that the crystallinity index increased from 44.8% to 48.2% due to the hydrolysis of the amorphous region in COC. The results of colorimetry also reveal an efficient hydrolysis process. A co-culture of B. tropicus and Saccharomyces cerevisiae was used to produce ethanol from COC waste, and the maximum ethanol yield was 4.2 g/L. The results of this work show that B. tropicus can be used to prepare biotechnology value-added products such as biofuels from lignocellulosic biomass, suggesting promising utility in biotechnology applications. Full article

This study addresses the complex challenges associated with road traffic flow prediction and congestion management through the enhancement of the attention-based spatiotemporal graph convolutional network (ASTGCN) algorithm. Leveraging toll data and real-time traffic flow information from Orange County, California, the algorithm undergoes refinement to adeptly capture abrupt changes in road traffic dynamics and identify instances of acute congestion. The optimization of the graph structure is approached from both macro and micro perspectives, incorporating key factors such as road toll information, node connectivity, and spatial distances. A novel graph self-learning module is introduced to facilitate real-time adjustments, while an attention mechanism is seamlessly integrated into the spatiotemporal graph convolution module. The resultant model, termed AASTGNet, exhibits superior predictive accuracy compared to existing methodologies, with MAE, RMSE, and MAPE values of 8.6204, 14.0779, and 0.2402, respectively. This study emphasizes the importance of incorporating tolling schemes in road traffic flow prediction, addresses static graph structure limitations, and adapts dynamically to temporal variations and unexpected road events. The findings contribute to advancing the field of traffic prediction and congestion management, providing valuable insights for future research and practical applications. Full article

Lactobacillus casei (L. casei) has four possible mechanisms: antimicrobial antagonism, competitional adhesion, immunoregulation, and the inhibition of bacterial toxins. To delineate the metabolic reactions of nucleotides from L. casei that are associated with mechanisms of inhibiting pathogens and immunoregulation, we report that a PyrR-deficient L. casei strain was constructed using the CRISPR-Cas9^D10A tool. Furthermore, there were some changes in its basic biological characterization, such as its growth curve, auxotroph, and morphological damage. The metabolic profiles of the supernatant between the PyrR-deficient and wild strains revealed the regulation of the synthesis of genetic material and of certain targeting pathways and metabolites. In addition, the characteristics of the PyrR-deficient strain were significantly altered as it lost the ability to inhibit the growth of pathogens. Moreover, we identified PyrR-regulating pyrimidine biosynthesis, which further improved its internalization and colocalization with macrophages. Evidence shows that the PyrR gene is a key active component in L. casei supernatants for the regulation of pyrimidine biosynthesis against a wide range of pathogens. Full article

In this study, virgin coconut oil (VCO) nanoemulsions were prepared by ultrasonication using a sodium caseinate (SC) and xanthan gum (XG) complex as an emulsifier. The stability and digestion characteristics of SC/XG–VCO emulsions formed by co-adsorption and SC–VCO–XG emulsions formed by layer adsorption were compared. The stability of the two emulsions was studied under different pH, ionic strength, heat treatment, freeze–thaw cycles, and storage conditions, and the droplet size and zeta potential were used as indicators to assess the stability. In addition, the stability of oxidation and the digestive properties of both emulsions were studied. It was found that the SC–VCO–XG emulsions had better environmental stability, oxidative stability, storage stability, and digestibility compared to SC/XG–VCO emulsions. This study has shown that the formation method of protein–polysaccharide stabilized emulsions has an impact on the stability and digestibility properties of the emulsions, and that the emulsion carriers constructed by layer adsorption are more suitable for subsequent industrial production and development. Full article

Heterotrophic nitrification–aerobic denitrification (HN-AD) is an efficient nitrogen removal process and the genus Paracoccus is one important group of the HN-AD bacteria. During an investigation of the microbial diversity in marine ranching of the Pearl River Estuary (PR China), three bacterial strains, designated SCSIO 75817^T, SCSIO 76264^T and SCSIO 80058^T, were isolated from sediments. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the three strains belonged to the genus Paracoccus and their closest neighbors were P. isoporae DSM 22220^T (97.6–98.0%), P. aurantiacus CGMCC 1.13898^T (97.3–97.6%) and P. xiamenensis MCCC 1A16381^T (97.1–97.4%), respectively. The analysis results of 16S rRNA gene similarity, ANI, AAI and dDDH showed that the pairwise similarities between these three strains and their closest neighbors were 97.4–98.5%, 76.9–81.0%, 75.5–79.6% and 20.3–23.3%, respectively. Polyphasic taxonomic data of the phylogenetic, phenotypic and chemotaxonomic analyses indicate that these strains represent three novel species in the genus Paracoccus, for which the names Paracoccus aerodenitrificans sp. nov., Paracoccus sediminicola sp. nov. and Paracoccus albus sp. nov. are proposed, respectively. The study also demonstrated the heterotrophic nitrification–aerobic denitrification (HN-AD) ability of the novel species P. aerodenitrificans SCSIO 75817^T. When it was aerobically cultivated at 28 °C using NH₄⁺-N, NO₃⁻-N and NO₂⁻-N as the sole nitrogen sources, the nitrogen removal efficiencies were 73.4, 55.27 and 49.2%, respectively, and the maximum removal rates were 3.05, 1.82 and 1.63 mg/L/h, respectively. The results suggest that it has promising potential for wastewater treatment. Full article

In China, the national-level protected pig, the Min pig, is characterized by the development of secondary hairs and hair follicles in winter. Factors that dominate the genotype in the growth of secondary hairs are not clear through the concrete cell signaling pathways. This study compared hair phenotypes based on morphological structure, transcriptomics, and potential targeting molecules in the breeds of Min, Berkshire, and Yorkshire pigs. The results indicated that Min pigs have specific characteristics for the growth of secondary hairs compared with the Berkshire and Yorkshire pigs. The transcriptome analyses and quantitative reverse transcription-polymerase chain reaction results revealed that secondary hair growth was activated by follicle stem cells. The specific inhibitors of Wnt and BMP were studied using respective signals. The density of follicles, activity of follicle stem cells, and relative gene expression results have shown that Wnt and BMP stimulate the activity of follicle stem cells, and the Wnt signaling molecule has a significantly better effect than the BMP signaling molecule on stem cells. Wnt and BMP can promote the growth of local secondary hair and gene expression. Therefore, this study was conducted to verify the development mechanisms of secondary hairs, which have potential applications in laboratory animals and comparative medicine. Full article

For exploring structurally diverse metabolites and uniquely metabolic mechanisms, we systematically investigated the chemical constituents and putative biosynthesis of Janibacter sp. SCSIO 52865 derived from the deep-sea sediment based on the OSMAC strategy, molecular networking tool, in combination with bioinformatic analysis. As a result, one new diketopiperazine (1), along with seven known cyclodipeptides (2–8), trans-cinnamic acid (9), N-phenethylacetamide (10) and five fatty acids (11–15), was isolated from the ethyl acetate extract of SCSIO 52865. Their structures were elucidated by a combination of comprehensive spectroscopic analyses, Marfey’s method and GC-MS analysis. Furthermore, the analysis of molecular networking revealed the presence of cyclodipeptides, and compound 1 was produced only under mBHI fermentation condition. Moreover, bioinformatic analysis suggested that compound 1 was closely related to four genes, namely jatA–D, encoding core non-ribosomal peptide synthetase and acetyltransferase. Full article

Four actinomycete strains isolated from the coral Acropora austera and coral sand samples from the South China Sea, were found to produce a series of halogenated compounds baring similar ultraviolet absorption based on the analysis of HPLC and LC-MS. The production titers of halogenated compounds from Streptomyces diacarni SCSIO 64983 exceeded those of other similar strains leading us to focus on SCSIO 64983. Four new thiocarbazomycins A–B (1–2), chlocarbazomycin E (3), and brocarbazomycin A (4), together with three known chlocarbazomycins A–C (5–7) containing a carbazole core were identified, and their structures were determined using a combination of spectroscopic analysis including HRESIMS, 1D and 2D NMR. Structurally speaking, compounds 1 and 2 have the rare sulfur-containing carbazole nuclei, and 3 and 4 contain Cl and Br atoms, respectively. Although these compounds have not yet been found to have obvious biological activity, their discovery highlights the role of molecular libraries in subsequent drug discovery campaigns. Full article