Search Results (130)

The cytokinin response regulator (ARR) gene is essential for cytokinin signal transduction, which plays a crucial role in plant growth and development. However, the functional mechanism of ARR genes in cotton leaf abscission remains incompletely understood. In this study, a total of 86 ARR genes were identified within the genome of Gossypium hirsutum. These genes were categorized into four distinct groups based on their phylogenetic characteristics, supported by analyses of gene structures and conserved protein motifs. The GhARR genes exhibited an uneven distribution across 25 chromosomes, with three pairs of tandem duplication events observed. Both segmental and tandem duplication events significantly contributed to the expansion of the ARR gene family. Furthermore, numerous putative cis-elements were identified in the promoter regions, with hormone and stress-related elements being common among all 86 GhARRs. Transcriptome expression profiling screening results demonstrated that GhARRs may play a mediating role in cotton’s response to TDZ (thidiazuron). The functional validation of GhARR16, GhARR43, and GhARR85 using virus-induced gene silencing (VIGS) technology demonstrated that the silencing of these genes led to pronounced leaf wilting and chlorosis in plants, accompanied by a substantial decrease in petiole fracture force. Overall, our study represents a comprehensive analysis of the G. hirsutum ARR gene family, revealing their potential roles in leaf abscission regulation. Full article

The purpose of this research is to clarify the genetic groups associated with high-quality germplasms with different genotypes and investigate the responses of their yield and quality traits to varied fertilization regimes, thereby providing references for premium breeding and cultivation practices. A total of 29 samples, including 15 newly developed lines and 14 elite cultivars, were analyzed as to their genetic structure. A split-plot field experiment was conducted to evaluate the responses of yield, quality, and agronomic traits to different fertilization treatments. The 29 samples were classified into three genetic groups. Line 1, developed by our team, formed a unique group, while Lines 3 and 8 constituted another distinct group. In the 2022 trial, the protein content ranged from 6.8% to 9.0%, amylose content from 17.9% to 20.1%, and taste value from 73.8 to 85.7. The top five samples in yield were Line 4, Line 2, Line 5, Line 6, and Line 1, with significant differences among them (p < 0.05) and markedly higher yields compared to other samples. For taste value, Longdao 17029, Line 2, Line 4, Line 5, and Line 6 ranked highest, with Longdao 17029 exhibiting significantly superior taste scores (p < 0.05). Fertilizer treatments ranked by yield were as follows: Treatment 1 (N₁₂₀P₁₀₀K₁₀₀) > Treatment 4 (Longfutianxia slow-release fertilizer) > Treatment 2 (N₉₆P₁₀₀K₁₀₀ + bio-organic fertilizer) > Treatment 3 (N₀P₀K₀), with significant differences (p < 0.05). Treatment 2 achieved a significantly higher taste value than the others (p < 0.05). In the 2023 trial, Longdao 17029 demonstrated higher leaf color index and net photosynthetic rate during the grain-filling stage, compared to Longdao 18 and Longdao 21. Lines 1, 3, and 8 are valuable for broadening the genetic diversity of rice in cold regions. Line 4 and Longdao 17029 show potential as novel germplasms for improving yield and taste quality. Moderate nitrogen reduction, combined with bio-organic fertilizer application, enhances rice taste value. Full article

High-precision clock offset products directly affect the performance and reliability of precise point positioning (PPP) applications. Currently, real-time clock offset products offered by institutions such as the Centre national d’études spatiales (CNES) rely on ultra-rapid predicted orbits. However, these orbits have limited accuracy and exhibit jumps during updates, constraining the accuracy of real-time clock estimation. To address this issue, we propose an undifferenced ambiguity resolution (UD AR) technique for clock offset estimation based on epoch-wise updated orbits. Clock estimation experiments were performed using both predicted and epoch-wise updated orbits, with square root information filtering (SRIF) applied in three schemes: double-differenced (DD), UD, and float solutions. Compared with predicted orbits, epoch-wise updated orbits provided smoother sequences with higher accuracy, significantly improving clock offset estimation accuracy in all schemes. Moreover, the UD AR solution significantly enhanced clock offset estimation accuracy, and the high-precision epoch-wise updated orbit products increased the narrow-lane fixing rate of the UD solutions. The clock accuracies of BDS-3, Galileo, and GPS reached 0.032 ns, 0.023 ns, and 0.026 ns, respectively, representing improvements of 36%, 34%, and 41% compared with the float solutions and 41%, 30%, 26% compared with the UD solution based on 1 h predicted orbits. Finally, the positioning performance of the proposed method was validated via PPP using 25 stations, showing improvements of 50%, 48%, and 41% in the north, east, and up directions compared with CNES products. Therefore, by combining epoch-wise updated orbit products with the UD AR to improve clock accuracy, this method provides a new approach to generating high-precision clock products, significantly contributing to enhancing PPP services. Full article

This study examined the genetic and evolutionary features of influenza A/H3N2 viruses in Hangzhou (2010–2022) by analyzing 28,651 influenza-like illness samples from two sentinel hospitals. Influenza A/H3N2 coexisted with other subtypes, dominating seasonal peaks (notably summer). Whole-genome sequencing of 367 strains was performed on GridION platforms. Phylogenetic analysis showed they fell into 16 genetic groups, with multiple clades circulating simultaneously. Shannon entropy indicated HA, NA, and NS gene segments exhibited significantly higher variability than other genomic segments, with HA glycoprotein mutations concentrated in antigenic epitopes A–E. Antiviral resistance showed no inhibitor resistance mutations in PA, PB1, or PB2, but NA mutations were detected in some strains, and most strains harbored M2 mutations. A Bayesian molecular clock showed the HA segment exhibited the highest nucleotide substitution rate (3.96 × 10⁻³ substitutions/site/year), followed by NA (3.77 × 10⁻³) and NS (3.65 × 10⁻³). Selective pressure showed A/H3N2 strains were predominantly under purifying selection, with only sporadic positive selection at specific sites. The Pepitope model demonstrated that antigenic epitope mismatches between circulating H3N2 variants and vaccine strains led to a significant decline in influenza vaccine effectiveness (VE), particularly in 2022. Overall, the study underscores the complex circulation patterns of influenza in Hangzhou and the global importance of timely vaccine strain updates. Full article

Despite free access to complete information regarding hotel quality and reference prices, consumers perceive significant price differences across different online platforms. We explore how perceived price dispersion on online travel agency platforms influences consumer purchase intention through mental account theory and propose a psychological mechanism explaining why consumers may tolerate and even embrace price discrepancies. Study 1 employs a scenario-based experiment that manipulates differing levels of price dispersion for the same hotel booking, demonstrating that higher PPD significantly amplifies perceived transaction utility and, in turn, acquisition utility. Study 2 corroborates these findings through an online survey with judgment sampling, highlighting that consumers—despite access to comprehensive OTA information—are often motivated, rather than deterred, by price discrepancies; multiple variable combinations were tested to ensure robust findings. This study challenges traditional marketing theories suggesting that price dispersion signals market unfairness and reduces consumers’ purchasing intention; instead, it mentally stimulates consumers. This perception enhances transaction and acquisition utility, positively impacting purchase intention. We also offer a robust model for mechanism study and provide insights for leveraging price dispersion as a cost-less promotional strategy, potentially increasing consumer engagement without additional marketing expenditure. We contribute to the literature by integrating the mental account theory into the context of online marketplaces and developing a price dispersion model with psychological utility in the consumer decision-making process. Full article

New quality productivity in agriculture and rural areas serves as a critical foundation for addressing the development needs of the times, advancing the comprehensive revitalization of rural regions, and overcoming the urban–rural dual structure. This paper studies the spatial changes and evolutionary trends of new quality productivity in agriculture and rural areas in 31 provinces of mainland China. The main conclusions are as follows: (1) At the provincial level, the development of China’s new quality productivity exhibits a spatial gradient, with a decreasing trend from east to west. (2) At the national scale, while significant spatial autocorrelation exists in the new quality productivity of agriculture and rural areas, internal disparities are gradually narrowing. (3) The eastern region demonstrates significant advantages, the central region is making steady progress, the western region is rapidly catching up, and the northeastern region faces increasingly significant development pressures. This paper extends the research boundary of new quality productivity to the field of agriculture and rural areas, and we introduce a variety of spatial analysis methods to depict its distribution characteristics. Full article

The water exchange capacity (WEC) in semi-enclosed bays is influenced by various dynamical processes. Among them, the wave effects are important and yet not well-understood. In this study, the Dalian Bay, a typical coastal semi-enclosed bay located in northeastern China, was chosen as an example, and the finite volume community ocean model (FVCOM) coupled with a wave module has been employed to investigate the wave effects on WEC in the Dalian Bay in the summer. The Dalian Bay is composed by three small-sized inner bays, Tianshuitao (TST), Hongtuduizi (HTDZ) and Choushuitao (CST), as well as the central part of the Dalian Bay (CPDB). The model performance has been evaluated comprehensively by comparing a suite of quantitative metrics, procedures and spatiotemporal patterns between the simulated results and time series current and wave measurements. The simulated results well-reproduced the observations, justifying the model’s ability in reproducing the hydrodynamics of the research region. The model results and observation all indicated that the averaged current velocities in the Dalian Bay were increased by about 0.1–0.2 m/s under wave effects during one strong wave event. Especially in the TST, the current velocities were increased most significantly. Moreover, dyed tracer experiments have been conducted to investigate the wave effects on WEC, and half-life time of different subregions of the Dalian Bay were also calculated. The results showed regional differences in wave effects. Overall, the impacts of waves were more significant in the northern part of the Dalian Bay. In the summer, southeast winds prevail, which generate waves in the southeast directions. Facilitated by regional geographical settings, waves could reach the northern part directly, which reduced the dyed tracer concentrations substantially, signifying a stronger WEC. Therefore, waves exerted the greatest impacts on the TST and reduced the local half-life time by about 10–20 days through increasing the efficiency of material transports. And the half-life time of the HTDZ, when considering the wave effects, was reduced by 15 days. However, confined by the twisting coastline, the wave effects on WEC in the CPDB and the CST were not that distinguished compared to the other parts of the bay. Full article

Surface albedo measurements of snow and ice on Lake Ulansu in the Central Asian arid climate zone were conducted during the winter of 2016–2017. Observations were categorized into three stages based on the ice growth and surface condition: bare ice, snow cover, and melting. During the bare ice stage, the mean surface albedo was 0.35 with a decreasing trend due to the accumulation of wind-blown sediment on the ice surface (range: 0.99–1.87 g m⁻²). Two snowfall events occurred during the snow cover stage, significantly increasing the surface albedo to 0.91. During the melting stage, the albedo decreased at a decay rate of 0.20–0.30/day. Four existing albedo schemes were evaluated but found unsuitable for Lake Ulansu. A new surface albedo scheme was proposed by incorporating the existing albedo schemes with the measured data. This scheme incorporated the effect of sediment content on bare ice albedo for the first time. It demonstrated a modelling efficiency of 0.933 over the entire 3-month period, which was used to evaluate the fit between the predicted and observed values. When validated with albedo observations from other winters, it achieved a modelling efficiency of 0.940. The closer the value is to 1, the better the model’s predictive accuracy, indicating a higher level of reliability in the model’s performance. This scheme has potential applicability to other lakes in the Central Asian arid climate zone, which is characterized by low precipitation, frequent sandstorms, and intense solar radiation. Full article

As a kind of bioactive component in the rhizome of natural plant Curcuma longa L. (turmeric), curcumin is almost insoluble in water at neutral and acidic pH, which limits its further utilization and development. At the same time, traditional extraction and separation processes typically require the use of a large number of organic solvents. Ionic liquids (ILs) are organic molten salts with melting points below 100 °C. When an ionic liquid exists in a liquid state at or near room temperature, it is referred to as a room-temperature ionic liquid (RTIL). They have a temperature range, good physical and chemical stability, and good structural designability. They have a strong solubilization enhancement effect for many organic compounds. This study first explored the molecular forms of curcumin in ionic liquid aqueous solutions and the intermolecular interactions between curcumin and ionic liquids using spectral analysis and computational chemistry methods; furthermore, using an ionic liquid aqueous solution as an extraction agent, curcumin-like substances (curcuminoids) were extracted from turmeric powders under ultrasound assisted conditions, revealing the relationship between the structure of the ionic liquid and the extraction efficiency. After that, a kinetic study was conducted for the extraction of curcuminoids from turmeric powders, using second-order kinetics fitting to obtain the rate constant and initial extraction rate during the extraction process. Finally, the comparison with a ComplexGAPI tool and antioxidant experiment was performed on the extraction by using ionic liquids and traditional solvent. The full results can provide reference for the design of IL extractants and their application for natural products. Full article

Antimonide laser diodes, with their high performance above room temperature, exhibit significant potential for widespread applications in the mid-infrared spectral region. However, the laser’s performance significantly degrades as the emission wavelength increases, primarily due to severe quantum-well hole leakage and significant non-radiative recombination. In this paper, we put up an active region with a high valence band offset and excellent crystalline quality with high luminescence to improve the laser’s performance. The miscibility gap of the InGaAsSb alloy was systematically investigated by calculating the critical temperatures based on the delta lattice parameter model. As the calculation results show, In_0.54Ga_0.46As_0.23Sb_0.77, with a compressive strain of 1.74%, used as the quantum well, is out of the miscibility gap with no spinodal decomposition. The quantum wells exhibit high crystalline quality, as evidenced by distinct satellite peaks in XRD curves with a full width at half maximum (FWHM) of 56 arcseconds for the zeroth-order peak, a smooth surface with a root mean square (RMS) roughness of 0.19 nm, room-temperature photoluminescence with high luminous efficiency and narrow FHWM of 35 meV, and well-defined interfaces. These attributes effectively suppress non-radiative recombination, thereby enhancing internal quantum efficiency in the antimonide laser. Furthermore, a novel epitaxial laser structure was designed to acquire low optical absorption loss by decreasing the optical confinement factor in the cladding layer and implementing gradient doping in the p-type cladding layer. The continuous-wave output power of 310 mW was obtained at an injection current of 4.6 A and a heatsink temperature of 15 °C from a 1500 × 100 μm² single emitter. The external quantum efficiency of 53% was calculated with a slope efficiency of 0.226 W/A considering both of the uncoated facets. More importantly, the lasing wavelength of our laser exhibited a significant blue shift from 3.4 μm to 2.9 μm, which agrees with our calculated results when modeling the interdiffusion process in a quantum well. Therefore, the interdiffusion process must be considered for proper design and epitaxy to achieve mid-infrared high-power and high-efficiency antimonide laser diodes. Full article

Gas channeling treatment is a huge challenge for oil displacement and CO₂ sequestration in the practical CO₂ flooding process. The foaming agents can be used in the gas flooding process, which presents good application potential for gas channeling blockage. However, high temperature can affect surfactant foaming properties. This work takes a high-temperature heterogenous sandstone oil reservoir as an example; the foaming performance of different surfactants was evaluated via foamability, thermal stability, crude oil tolerance ability, and dynamic blocking capacity. The profile control performance of the optimized foaming agent was investigated via dual-core gas flooding experiments. (1) The results show that QPJ-c featured good foaming stability, which made it present the largest foam comprehensive index, although its foaming volume was slightly lower than that of QPJ-b. Its foaming volume retention rate was 83.2%, and its half-life retention rate remained 88.9% after 30 days aging at a temperature of 110 °C. (2) The foam resistance factor increased from 7 to 17 when the core permeability increased from 2 mD to 20 mD. This indicated that the high-permeability zone could be preferentially blocked by foam during the foam injection. (3) The dual-core flooding experiments verified that the fractional flow of the high-permeability core severely decreased due to the blockage of foam. The incremental oil recovery of the low-permeability core was 27.1% when the permeability ratio was 5. It increased to 40% when the permeability ratio was increased to 10. (4) Our work indicates that temperature-resistant CO₂ foam could be a good candidate for profile control during CO₂ flooding in the target reservoir. Full article

We reported on a single-longitudinal-mode operated distributed Bragg reflector laser diode emitting at 1950 nm with an on-chip integrated power amplifier. Second-order Chromium–Bragg gratings are carefully designed and fabricated at the end of the ridge waveguide. Achieving a stable single-mode operation with a large injecting current range of 800 mA from 15 °C to 40 °C. The maximum side-mode suppression ratio (SMSR) is up to 42 dB. To increase the output power, an on-chip integrated master oscillator power amplifier (MOPA) is also introduced. MOPA-DBR lasers with different matching configurations between the gain peak and Bragg wavelength are fabricated, resulting in various amplification consequences. The best device is realized with 40 nm red-shifted between Bragg wavelength and photoluminescence (PL) peak. A power amplification of 5.6 times is achieved with the maximum output power of 45 mW. Thus, we put up the feasibility and key design parameters of on-chip integrated power amplification DBR lasers towards mid-infrared. Full article

As rice yield rises, the issue of broken axis windings in mature rice threshing drums is becoming increasingly severe. To disclose the winding characteristics and signal traits of the drums during the threshing process of mature rice, this paper undertakes an analysis of the winding characteristics and signals of the drums in rice with the assistance of a vibration test and analysis system. Since rice can lead to drum winding and shaft breakage, this paper alters the driving mode of the drums to exhibit the influence of rice on them. Firstly, the transfer characteristics of the frame need to be studied and analyzed, followed by subsequent research. The test results indicate that the horizontal displacement of the cylinder axial trajectory rises with the growth of the transmission chain, while the vertical displacement drops with the growth of the transmission chain. Additionally, to investigate the effect of mature rice on the threshing performance of horizontal axis II and horizontal axis III, a control variable method was employed. This approach allowed for the observation of how mature rice influences the threshing roller by systematically adjusting both the threshing gap and the rotational speed of the roller. It can be discerned from the test results that with the reduction of the threshing gap of affected mature rice, the unstripped rate gradually declines, while the entrainment loss rate gradually increases. As the rotational speed of horizontal shaft roller III increases, the rate of uncleared material gradually decreases, while the rate of entrainment loss progressively rises. The research findings can furnish a reference for the winding characteristics of rice on rollers and the enhancement of vehicle driving comfort. Full article

While adult fireflies are terrestrial, their larvae inhabit various habitat types, and a lack of comprehensive research on the feeding habits of these larvae across different habitats has greatly impeded the development of artificial diets. Here, we tested 14 types of foods, primarily covering gastropods, vertebrates, and fruit, to survey feed for aquatic (Aquatica leii and Sclerotia substriata), semi-aquatic (Pygoluciola qingyu and Pygoluciola sp.), and terrestrial (Pyrocoelia analis) fireflies. The results show that A. leii, S. substriata, P. qingyu, Pygoluciola sp., and P. analis fed on 12, 6, 10, 10, and 7 different foods, respectively, showing an obvious difference in feeding range among various inhabit types of fireflies. Aquatic lineages preferred to consume freshwater snails, followed by pork meat and land slugs, while semi-aquatic fireflies favored freshwater snails, followed by fish and shrimp. Favorite foods were thus freshwater snails such as Cipangopaludina chinensis for both aquatic and semi-aquatic fireflies, but feeding preference differed for second favorite foods (e.g., pork vs. fish meat). Terrestrial Pyrocoelia analis showed different feeding preferences compared withthe other two habitat lineages, with terrestrial snails and slugs as their favorite foods, followed by freshwater snails, such as C. chinensis. These findings not only uncovered larval feeding habits of fireflies across various inhabit types but also indicated that readily available and affordable C. chinensis products can serve as wild snail alternatives in the artificial feeding of fireflies. This study is the first to explore the feeding habits of firefly species inhabiting water and land habitat types, adding to the understanding of the feeding characteristics of fireflies. Full article

The monitoring of acetone in exhaled breath is expected to provide a noninvasive and painless method for dynamic monitoring of summarized physiological metabolic status during obesity treatment. Although the commonly used Mass Spectrometry (MS) technology has high accuracy, the long detection time and large equipment size limit the application of daily bedside detection. As for the real-time and accurate detection of acetone, the gas sensor has become the best choice of gas detection technology, but it is easy to be disturbed by water vapor in breath gas. An integrated breath gas detection system based on cavity ring-down spectroscopy (CRDS) is reported in this paper, which is a laser absorption spectroscopy technique with high-sensitivity detection and absolute quantitative analysis. The system uses a 266 nm single-wavelength ultraviolet laser combined with a breath gas pretreatment unit to effectively remove the influence of water vapor. The ring-down time of this system was 1.068 μs, the detection sensitivity was 1 ppb, and the stability of the system was 0.13%. The detection principle of the integrated breath gas detection system follows Lambert–Beer’s law, which is an absolute measurement with very high detection accuracy, and was further validated by Gas Chromatography–Mass Spectrometer (GC-MS) testing. Significant differences in the response of the integrated breath gas detection system to simulated gases containing different concentrations of acetone indicate the potential of the system for the detection of trace amounts of acetone. Meanwhile, the monitoring of acetone during obesity treatment also signifies the feasibility of this system in the dynamic monitoring of physiological indicators, which is not only important for the optimization of the obesity treatment process but also promises to shed further light on the interaction between obesity treatment and physiological metabolism in medicine. Full article