Search Results (256)

Trichogramma wasps are highly effective biological control agents, offering an environmentally sustainable solution for pest management through their parasitism of insect eggs. This study evaluates the parasitism performance of six Trichogramma species—T. dendrolimi, T. chilonis, T. leucaniae, T. ostriniae, T. japonicum, and T. pretiosum—on five treatments of Eri silkworm (ES) eggs, a potential alternative to the large eggs of Antheraea pernyi for mass rearing. The ES egg treatments included the following: manually extracted, unfertilized, and washed eggs (MUW); naturally laid, unfertilized, and washed eggs (NUW); naturally laid, unfertilized, and unwashed eggs (NUUW); naturally laid, fertilized, and washed eggs (NFW); and naturally laid, fertilized, and unwashed eggs (NFUW). The results demonstrate that all Trichogramma species, except T. japonicum, successfully parasitized ES eggs across all treatments. Notably, washed eggs consistently supported higher parasitism and emergence rates compared to unwashed eggs, while unfertilized eggs outperformed fertilized eggs in these metrics. Among the treatments, unfertilized and washed eggs (MUW and NUW) exhibited the shortest pre-emergence time and the highest number of emerged adults, with no significant differences in female progeny ratios across most species. A striking exception was T. dendrolimi, which showed a significantly higher female offspring ratio in the MUW treatment. These findings highlight that MUW eggs of ES are a highly suitable alternative host for the mass production of Trichogramma wasps. This study provides critical insights for optimizing host egg treatments to enhance the efficiency of Trichogramma-based biological control programs. Full article

Avian infectious bronchitis virus (IBV) infection has caused significant economic losses to the poultry industry. Unfortunately, there is currently no effective cure for this disease. Understanding the pathogenic mechanism is crucial for the treatment of the disease. Studying the pathogenic mechanism of IBV based on metabolomics analysis is helpful for identifying antiviral drugs. However, studies on metabolomics analysis of IBV infection have been relatively limited, particularly without metabolomics analysis in sera after IBV infection. In this study, 17-day-old SPF chicks were infected with the IBV GX-YL5 strain, and serum samples were collected 7 days post-infection (DPI) for metabolomics analysis using ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). A total of 143 differential metabolites were identified across 20 metabolic pathways, with the phenylalanine pathway showing the most significant changes. The level of cinnamic acid (CA), an upstream metabolite in the phenylalanine pathway, was notably increased following IBV infection. To investigate the antiviral effects of CA, chicken embryo kidney (CEK) cells and SPF chicks infected with IBV were treated with different concentrations of CA to assess its effect on viral replication. The results demonstrated that CA at 25 μg/mL effectively inhibited IBV replication in vitro; meanwhile, CA at 50 μg/mL and 25 μg/mL effectively inhibited IBV replication in vivo. Molecular docking and molecular dynamics simulation studies showed that CA interacts with the N domains of the IBV nucleocapsid (N) protein. In conclusion, the serum metabolite CA is significantly elevated following IBV infection and demonstrates remarkable antiviral effects both in vitro and in vivo, providing a promising avenue for the development of antiviral therapies to combat IBV infection. Full article

In this study, we integrated HSQC-based DeepSAT with UPLC-MS/MS to guide the isolation of omega-3 polyunsaturated fatty acid derivatives (PUFAs) from marine resources. Through this approach, four new (1–4) and nine known (5–13) PUFA analogues were obtained from large-scale cultures of the marine dinoflagellate Prorocentrum lima, with lipidomic profiling identifying FA18:5 (5), FA18:4 (7), FA22:6 (8), and FA22:6 methyl ester (11) as major constituents of the algal oil extract. Structural elucidation was achieved through integrated spectroscopic analyses of IR, 1D and 2D NMR, and HR-ESI-MS data. Given the pivotal role of microglia in Alzheimer’s disease (AD) pathogenesis, we further evaluated the neuroprotective potential of these PUFAs by assessing their regulatory effects on critical microglial functions in human microglia clone 3 (HMC3) cells, including chemotactic migration and amyloid-β42 (Aβ42) phagocytic clearance. Pharmacological evaluation demonstrated that FA20:5 butanediol ester (1), FA18:5 (5), FA18:4 (7), FA22:6 (8), and (Z)-10-nonadecenoic acid (13) significantly enhanced HMC3 migration in a wound-healing assay. Notably, FA18:4 (7) also significantly promoted Aβ42 phagocytosis by HMC3 microglia while maintaining cellular viability and avoiding pro-inflammatory activation at 20 μM. Collectively, our study suggests that FA18:4 (7) modulates microglial function in vitro, indicating its potential to exert neuroprotective effects. Full article

The hard-seed coat of Ormosia henryi significantly impedes germination efficiency in massive propagation, while conventional physical dormancy-breaking methods often result in compromised seed vigor, asynchronous seedling emergence, and diminished stress tolerance. Seed biopriming, an innovative technique involving the inoculation of beneficial microorganisms onto seed surfaces or into germination substrates, enhances germination kinetics and emergence uniformity through microbial metabolic functions and synergistic interactions with seed exudates. Notably, spermosphere-derived functional bacteria isolated from native spermosphere soil demonstrate superior colonization capacity and sustained bioactivity. This investigation employed selective inoculation of these indigenous functional strains to systematically analyze dynamic changes in endogenous phytohormones, enzymatic activities, and storage substances during critical germination phases, thereby elucidating the physiological mechanisms underlying biopriming-enhanced germination. The experimental results demonstrated significant improvements in germination parameters through biopriming. Inoculation with the Bacillus sp. strain achieved a peak germination rate (76.19%), representing a 16.19% increase over the control (p < 0.05). The biopriming treatment effectively improved the seed vigor, broke the impermeability of the seed coat, accelerated the germination speed, and positively regulated physiological indicators, especially amylase activity and the ratio of gibberellic acid to abscisic acid. This study establishes a theoretical framework for microbial chemotaxis and rhizocompetence in seed priming applications while providing an eco-technological solution for overcoming germination constraints in O. henryi cultivation. The optimized biopriming protocol addresses both low germination rates and post-germination growth limitations, providing technical support for the seedling cultivation of O. henryi. Full article

This study investigates the interaction between airflow and low-density bulk particles within vertical screw conveyors and examines its impact on conveying performance. A combined simulation approach integrating the Discrete Element Method and Computational Fluid Dynamics was employed to model both single-phase particle flow and gas–solid two-phase flow. A periodic model was developed based on the structural characteristics of the conveyor. Particle motion dynamics under both single-phase and coupled two-phase conditions were analyzed using EDEM and coupled Fluent-EDEM simulations. The effects of key operational parameters, including screw speed, filling rate, and helix angle, on mass flow rate were systematically evaluated. A comprehensive performance index was established to quantify conveying efficiency, and its validity was confirmed through analysis of variance on the regression model. Finally, the response surface methodology was applied to optimize parameters and determine the optimal combination of screw speed and filling rate to enhance mass flow efficiency. The results indicate that the gas–solid two-phase flow model provides a more accurate representation of real-world conveying dynamics. Future research may extend the model to accommodate more complex material conditions. Full article

Gait is considered a valuable biometric feature, and it is essential for uncovering the latent information embedded within gait patterns. Gait recognition methods are expected to serve as significant components in numerous applications. However, existing gait recognition methods exhibit limitations in complex scenarios. To address these, we construct a dual-Kinect V2 system that focuses more on gait skeleton joint data and related acoustic signals. This setup lays a solid foundation for subsequent methods and updating strategies. The core framework consists of enhanced ensemble learning methods and Dempster–Shafer Evidence Theory (D-SET). Our recognition methods serve as the foundation, and the decision support mechanism is used to evaluate the compatibility of various modules within our system. On this basis, our main contributions are as follows: (1) an improved gait skeleton joint AdaBoost recognition method based on Circle Chaotic Mapping and Gramian Angular Field (GAF) representations; (2) a data-adaptive gait-related acoustic signal AdaBoost recognition method based on GAF and a Parallel Convolutional Neural Network (PCNN); and (3) an amalgamation of the Triangulation Topology Aggregation Optimizer (TTAO) and D-SET, providing a robust and innovative decision support mechanism. These collaborations improve the overall recognition accuracy and demonstrate their considerable application values. Full article

The ecological impacts of dam and reservoir construction necessitate systematic environmental quality evaluation (EEQ) to reconcile ecological protection with sustainable development. To address this need, we integrated the Remote Sensing Ecological Index (RSEI)—a comprehensive metric synthesizing greenness, humidity, heat, and dryness—with a Land Use Change Ecological Response (LUCER) model to quantify the long-term EEQ dynamics in reservoir-affected regions. This study utilized Landsat and Sentinel-2 remote sensing imagery with a 10 m resolution from the years 2000, 2005, 2010, 2015, and 2020 to compute the RSEI for the Qianping Reservoir area in Henan Province, investigating the spatiotemporal variations in EEQ. Key findings reveal: (1) Temporal trend: EEQ showed fluctuating improvement, with RSEI projected to rise gradually until 2030. (2) Spatial pattern: A lower ecological quality in central reservoir zones contrasts with higher quality in surrounding mountainous areas. (3) Mechanism: The Land Use Change Ecological Response (LUCER) model reveals that the conversion of cultivated land to forestland and grassland drives significant EEQ improvements, counterbalancing the negative impacts of hydrological fragmentation caused by reservoir construction and urbanization. This study advances RSEI applications in reservoir ecology by establishing a coupled monitoring–prediction framework, providing actionable insights for dam-related ecological restoration and governance. Full article

This study investigated the preventive effects and mechanisms of Paeonia lactiflora pall stem and leaf extract (PLE) on oxidative stress-induced diarrhea in broilers, using a Diquat (DQ)-induced model. Results indicated that PLE significantly improved growth performance, increased average daily gain (ADG), reduced feed-to-gain ratio (F/G), and enhanced liver and kidney indices. PLE alleviated DQ-induced oxidative stress diarrhea by reducing the diarrhea rate by 63.84%, upregulating mRNA expression of MUC2, Claudin-1, ZO-1, and Occludin, and decreasing AST and ALT activities in serum. Additionally, PLE increased levels of CAT, SOD, GSH-Px, and GSH while reducing PCO and MDA levels in serum, intestine, and liver tissues. Furthermore, PLE increased acetic acid content and decreased propionic acid, butyric acid, and isobutyric acid contents. PLE also altered gut microbiota by up-regulated Bacteroidetes and Barnesiella and down-regulated Firmicutes and unclassified_o__Eubacteriales. Network pharmacology suggested that PLE acts via the PI3K-Akt-Nrf2 pathway, confirmed by up-regulated mRNA expression of PI3K, AKT, Nrf2, NQO1, and HO-1, and down-regulated Keap1 in intestinal and liver tissues. Correlation analysis revealed significant associations between Barnesiella and unclassified_o__Eubacteriales with short-chain fatty acids and PI3K-Akt-Nrf2 pathway-related genes. Thus, PLE prevents and alleviates oxidative stress-induced diarrhea in broilers by modulating the PI3K-Akt-Nrf2 pathway, regulating gut microbiota, and influencing short-chain fatty acids. Full article

Climate change, represented by global warming, significantly affects the structure and function of alpine wetland ecosystems. Investigating the response strategies of alpine wetland plants to temperature changes is fundamental to understanding how alpine wetlands cope with global warming. This study, conducted at the typical alpine wetland Napahai, uses the latest predictions from the Intergovernmental Panel on Climate Change (IPCC) and employs open–top chamber warming experiments (OTCs) to study the responses of typical alpine wetland plants, Zizania caduciflor and Sparganium stoloniferum, to simulated warming. The results indicate that simulated warming significantly reduced the photosynthetic capacity of Z. caduciflor, and obviously decreased the biomass accumulation of both Z. caduciflor and S. stoloniferum (p < 0.05). The mean annual temperature (MAT) and annual maximum temperature (max) are the primary temperature factors affecting the photosynthetic and biomass parameters. Specifically, the net photosynthetic rate, stomatal conductance, transpiration rate, the aboveground, underground, and total biomasses, and the nitrogen contents of aboveground and underground buds of Z. caduciflor all showed significant negative correlations with MAT and max (p < 0.05). The parameters of S. stoloniferum mainly showed significant correlations with max, with its underground biomass, total biomass, and root nitrogen content all showing significant negative correlations with max, while its fibrous root carbon content and underground bud phosphorus content showed significant positive correlations with max (p < 0.05). The results are consistent with previous studies in high–altitude regions, indicating that warming reduces the photosynthetic capacity and biomass accumulation of alpine wetland plants, a trend that is widespread and will lead to a decline in the productivity of alpine wetlands and changes in vegetation composition. The study can provide a case for understanding the response strategies of alpine wetlands in the context of climate change. Full article

Fresh wet vermicelli is highly susceptible to microbial contamination during storage as a result of its high moisture content and rich nutrients, which leads to spoilage and deterioration. In addition to exerting a great impact on the quality of the product, this results in significant economic losses and potential food safety risks. This work aimed to identify spoilage microorganisms via traditional culturing methods and molecular biology techniques. The effects of environmental factors such as temperature and pH on the growth and development of the dominant spoilage fungi were investigated, and the inhibitory effects of both chemical (potassium sorbate) and natural antimicrobial agents (chitooligosaccharides, chitosan, tea polyphenols, citric acid, and ε-polylysine hydrochloride) were evaluated. The results indicated that Penicillium crustosum was the major spoilage microorganism in fresh wet vermicelli, whose optimal growth temperature and pH was 28 °C and 7, respectively. While conidial germination began at 7 h, hyphal formation was only observed after 12 h. Moreover, the findings suggest that both natural and chemical antimicrobial agents can effectively inhibit the growth of P. crustosum, with ε-polylysine hydrochloride being the strongest antimicrobial agent. Overall, the findings of this study provide a scientific foundation for improving the preservation of fresh wet vermicelli, which is of great significance for extending its shelf life and enhancing food safety. Full article

Coastal historic and cultural districts are distinctive urban public spaces which reflect the urban cultural and historical narratives. As an important symbol, coastal historic and cultural districts’ building colors play a crucial role in enhancing the historic and cultural districts’ visual quality and shaping the urban coastal landscape. Japan was one of the earliest countries to research urban color and building color regulation. This study selected Mojiko in Japan as the study site using street-view images, semantic segmentation technology, and ColorImpact4 software to collect information about the street building color. Based on the Moon–Spencer (M–S) color coordination theory and applied psychology methods, this study evaluated the building color quality and street color harmony from objective and subjective perspectives. It explored the impact of the building color on the coastal historic and cultural districts’ landscape quality. The results indicated that the building color of Mojiko generally presented a harmonious and unified characteristic. Most street buildings were soft, warm color tones, with higher color harmony and continuity. However, there were some problems, such as colors disrupting the overall street color environment, or color combinations were overly uniform. This study proposed a comprehensive method for building color analysis and evaluation, which is a reference value for guiding the color planning and construction for coastal historic and cultural districts’ landscape control. Full article

Cancer constitutes a significant global health challenge, ranking among the leading contributors to worldwide mortality. The inherent limitations of conventional oncologic interventions, particularly their frequent inability to induce durable remissions in advanced malignancies, continue to drive transformative explorations into novel therapeutic paradigms. In recent years, bacteria-based therapies have gained recognition in the management of solid tumors. Compared to traditional therapeutic modalities, extensive research has demonstrated that bacteria possess remarkable anticancer properties. Gut bacteria, which naturally coexist within the human body, represent a unique category of living cells with inherent advantages for solid tumor treatment. These microorganisms are characterized by their relative safety, ease of cultivation, and potential for use in precision medicine through genetic modifications. Furthermore, gut bacteria exhibit diverse mechanisms of action against tumor cells, with different bacterial species potentially exerting synergistic effects. However, the precise anticancer mechanisms of these bacteria, particularly those of gut microbiota, require further detailed investigation. This review categorizes anticancer gut bacteria according to their effects on cancer cells and elucidates their anticancer mechanisms across five domains: modification of the tumor microenvironment, competitive inhibition, activation of immune cells, vectors for gene therapy, and production of bacterial anticancer biomolecules. Additionally, we discuss the potential challenges of utilizing different gut bacteria for cancer treatment, highlight their anticancer advantages, and suggest promising directions for future research. Ultimately, this review serves as a comprehensive guide for utilizing both natural and engineered gut bacteria as therapeutic agents against solid tumors in cancer treatment. Full article

The northwestern slope of the Dongdao Platform in the Xisha Sea exhibits a complex geomorphological structure. Utilizing high-resolution multibeam bathymetric data and 2D seismic profiles, this study systematically reconstructs the slope morphology and its evolutionary processes. The study area displays a distinct threefold zonation: the upper slope (160–700 m water depth) has a steep gradient of 15°–25°, characterized by deeply incised V-shaped channels and slump deposits, primarily shaped by gravity-driven erosion; the middle slope (700–1200 m water depth) features a gentler gradient of 10°–15°, where channels stabilize, adopting U-shaped cross-sections with the development of lateral accretion deposits; the lower slope (1200–1500 m water depth) exhibits a milder gradient of 5°–10°, dominated by a mixture of fine-grained carbonate sediments and hemipelagic mud–marine sediments originating partly from the open ocean and partly from the nearby continental margin. The slope extends from 160 m to 1500 m water depth, hosting the C1–C4 channel system. Seismic facies analysis reveals mass-transport deposits, channel-fill facies, and facies modified by bottom currents—currents near the seafloor that redistribute sediments laterally—highlighting the interplay between fluid activity and gravity-driven processes. The slope evolution follows a four-stage model: (1) the pockmark formation stage, where overpressured gas migrates vertically through chimneys, inducing localized sediment instability and forming discrete pockmarks; (2) the initial channel development stage, during which gravity flows exploit the pockmark chains as preferential erosional pathways, establishing nascent incised channels; (3) the channel expansion and maturation stage, marked by intensified erosion from high-density debris flows, resulting in a stepped longitudinal profile, while bottom-current reworking enhances lateral sediment differentiation; (4) the stable transport stage, wherein the channels fully integrate with the Sansha Canyon, forming a well-connected “platform-to-canyon” sediment transport system. Full article

Long-term exposure to arsenic, a prevalent environmental contaminant, has been implicated in the pathogenesis of various hepatic conditions. Hepatic stellate cells (HSCs) are central to the development of liver fibrosis. Recently, the involvement of interleukin-17 (IL-17) and the NOD-like receptor protein 3 (NLRP3) inflammasome in hepatic pathologies has attracted significant research interest. Hepatocyte pyroptosis, a form of programmed cell death, is a critical factor in the occurrence of inflammation. The objective of this study was to investigate the specific roles of IL-17 and NLRP3 in the arsenic-induced activation of HSCs through hepatocyte pyroptosis. We pretreated MIHA cells with MCC950 (1 and 5 μM) and secukinumab (10 and 100 nM) for 4 h, then with NaAsO₂ (25 μM) for 24 h at 37 °C under 5% CO₂. After incubation, the cell-culture supernatant was collected and mixed with serum-free high-glucose DMEM medium in a 1:1 ratio to prepare the conditioned medium, which was subsequently used for the culture of LX-2 cells. The results showed that exposure to NaAsO₂ induced hepatocellular pyroptosis, which led to the release of the inflammatory cytokines IL-18 and IL-1β and subsequent activation of HSCs. Treatment with the inhibitors MCC950 and secukinumab significantly reduced the secretion of Extracellular matrix (ECM) components and attenuated HSC activation. These results demonstrate that blocking the IL-17 and NLRP3 signaling pathways significantly reduces HSC activation and attenuates hepatic fibrogenesis. These results provide novel molecular targets for the prevention and treatment of arsenic-related liver fibrosis. Full article

In the field of sustainable energy conversion and storage technologies, copper-based complexes have become a research hotspot due to their efficient and stable catalytic performance. The development of bifunctional catalysts that can simplify catalytic steps, enhance efficiency, and reduce catalyst usage has become an important research area. In this study, we successfully synthesized two copper complexes with different geometries utilizing di(2-pyridyl) ketone as the ligand, [Cu^II₂L₂Cl₂]·0.5H₂O (1) and [Cu₄^IIL₄(OCH₃)₂](NO₃)₂ (2) (L = deprotonated methoxy-di-pyridin-2-yl-methanol), which can serve as homogeneous electrocatalysts for water oxidation and CO₂ reduction simultaneously. The turnover frequency (TOF) of complexes 1 and 2 for electrocatalytic water oxidation are 7.23 s⁻¹ and 0.31 s⁻¹ under almost neutral condition (pH = 8.22), respectively. Meanwhile, the TOF of complexes 1 and 2 for the catalytic reduction of CO₂ to CO are 4.27 s⁻¹ and 8.9 s⁻¹, respectively. In addition, both complexes remain essentially unchanged during the electrocatalytic water oxidation and electrocatalytic CO₂ reduction processes, demonstrating good stability. Structural analysis reveals that the distinct catalytic efficiencies originate from their geometric configurations: the binuclear structure of complex 1 facilitates proton-coupled electron transfer during water oxidation, whereas the tetranuclear architecture of complex 2 enhances CO₂ activation. Complexes 1 and 2 represent the first two copper molecular electrocatalysts capable of catalyzing both water oxidation and CO₂ reduction. The findings in this work can open up new avenues for the advancement of artificial photosynthesis simulation and the development of bifunctional catalysts for water oxidation and CO₂ reduction. Full article