Search Results (42)

Being an agricultural country, Pakistan requires lots of water for irrigation. A major portion of its water resources is located in the upper indus basin (UIB). The snowmelt runoff generated from high-altitude areas of the UIB provides inflow into the Indus river system that boosts the water supply. Snow accumulation during the winter period in the highlands in the watershed(s) becomes a source of water inflow during the snow-melting period, which is described according to characteristics like snow depth, snow density, and snow water equivalent. Snowmelt water release (SWE) and snowmelt water depth (SD) maps are generated by tracing snow occurrence from MODIS-based images of the snow-cover area, evaluating the heating degree days (HDDs) from MODIS-derived images of the land surface temperature, computing the solar radiation, and then assimilating all the previous data in the form of the snowmelt model and ground measurements of the snowmelt water release (SWE). The results show that the average snow-cover area in the Astore river basin, in the upper indus basin, ranges from 94% in winter to 20% in summer. The maps reveal that the annual average values of the SWE range from 150 mm to 535 mm, and the SD values range from 600 mm to 2135 mm, for the snowmelt period (April–September) over the years 2010–2020. The areas linked with vegetation experience low SWE accumulation because of the low slopes in the elevated regions. The meteorological parameters and basin characteristics affect the SWE and can determine the SD values. Full article

Predictive maintenance of induction motors continues to be a significant challenge in ensuring industrial reliability and minimizing downtime. In this study, machine learning techniques are utilized to enhance fault diagnosis through the use of the Machinery Fault Database (MAFAULDA). A detailed extraction of statistical features was performed on multivariate time-series data to capture essential patterns that could indicate potential faults. Three machine learning algorithms—deep neural networks (DNNs), support vector machines (SVMs), and K-nearest neighbors (KNNs)—were applied to the dataset. Optimization strategies were carefully implemented along with oversampling techniques to improve model performance and handle imbalanced data. The results achieved through these models are highly promising. The SVM model demonstrated an accuracy of 95.4%, while KNN achieved an accuracy of 92.8%. Notably, the combination of deep neural networks with fast Fourier transform (FFT)-based autocorrelation features produced the highest performance, reaching an impressive accuracy of 99.7%. These results provide a novel approach to machine learning techniques in enhancing operational health and predictive maintenance of induction motor systems. Full article

The emergence of hypervirulent and carbapenem-resistant hypermucoviscous Klebsiella pneumoniae strains presents a significant public health challenge due to their increased virulence and resistance to multiple antibiotics. This study evaluates the antibiotic susceptibility patterns and virulence profiles of classical and hypervirulent K. pneumoniae strains isolated from various clinical samples. A total of 500 clinical samples were collected from patients at the Mardan Medical Complex and Ayub Medical Complex in KPK between July 2022 and June 2024. Among these, 64 K. pneumoniae strains were isolated and subsequently subjected to antimicrobial susceptibility testing (AST) and phenotypic virulence detection. Among the 64 isolates, 21 (32.8%) exhibited hypermucoviscosity, a characteristic associated with increased pathogenicity. Hemagglutination was observed in 35 (54.1%) of the isolates, indicating the presence of surface adhesins that facilitate bacterial adherence to host tissues. A high prevalence of biofilm formation was noted, with 54 (84%) isolates capable of forming biofilms, which are known to protect bacteria from antibiotics and the host immune response. Most isolates (59/64, 92.1%) were resistant against ampicillin, highlighting its limited efficacy against these strains. Conversely, the lowest resistance was observed for tigecycline, with only 15% (10/64) of the isolates showing resistance, indicating its potential utility as a treatment option. The study also found that 38 (59.3%) of the isolates were extended-spectrum beta-lactamase (ESBL) producers, 42 (65.6%) were multidrug-resistant (MDR), 32 (50%) were extensively drug-resistant (XDR), and 13 (20.3%) were resistant to carbapenems. The genetic study revealed biofilm producer and enhancer genes (mrkD, pgaABCD, fimH, treC, wzc, pilQ, and luxS) mainly in the hypervirulent strains. These hypervirulent strains also show a high number of resistance genes. The findings of this study underscore the critical need for the active surveillance of antimicrobial resistance and virulence determinants in K. pneumoniae. The coexistence of high levels of antibiotic resistance and virulence factors in these isolates poses a severe threat to public health, as it can lead to difficult-to-treat infections and increased morbidity and mortality. Full article

The evolution of Jurassic carbonates is globally significant for understanding the depositional framework, diagenetic phases and sedimentary characteristics of shallow marine shelf deposits. For this purpose, two outcrop sections of the Jurassic carbonates with a road distance of 121 km in the Trans Indus Ranges, NW Himalayas, were included in this study. Geological fieldwork was conducted for sedimentological data, and representative samples were collected for microfacies analysis and diagenetic evolution complemented by carbon and oxygen isotope analysis. Results show that eight microfacies were identified in both sections where mudstone microfacies was only present in the Chichali section, whereas wackestone and packstone facies widely existed in both sections. The diagenetic evolution interpreted that dolomitization and stylolization were pronounced in the Paniala section, while micritization and calcite cementation were prevalent in the Chichali section. The interpreted depositional setting implies the wide range from supratidal to outer ramp shallow marine for the Chichali section, suggesting a wide range and relatively deeper environment, alongside merely intertidal to middle ramp settings for Paniala section. Diagenetic evolution suggests marine to meteoric influence in the Chichali section, while burial and uplift phases were dominant in the Paniala section. The diagenetic events were also validated by the isotopic analysis, where most of the samples with values up to −4‰ VPDB δ¹⁸O, corresponding to a carbon isotope range of up to +4‰, were interpreted as the burial phase of diagenesis; meanwhile, a few samples with −2 δ¹³C and −7‰ VPDB δ¹⁸O isotope signatures were marked as meteoric influx in the Paniala section. This study indicates the diversity of the depositional environment and diagenetic heterogeneity by integration of thin sections using isotope data, which are quite applicable to shallow marine carbonates. Full article

Background: Typhoid fever caused by Salmonella enterica serovar Typhi (S. typhi) continues to pose a significant risk to public health in developing countries, including Pakistan. This study investigated the epidemiological factors linked to suspected and confirmed S. typhi infections in Peshawar’s hospital population. Methodology: A total of 5735 blood samples of patients with suspected enteric fever were collected from September 2022 to November 2023. S. typhi infection was confirmed using microbiological culture of blood samples, biochemical-based tests, and DNA-sequencing methods. Drug sensitivity testing on cultures was conducted as per the CLSI guidelines. Chi-square tests were used to analyze the clinical and epidemiologic characteristics of 5735 samples stratified by S. typhi infection status, and risk factors were assessed by applying logistic regression models to estimate odds ratios (ORs). Results: The number of confirmed typhoid fever cases in this hospital-based study population was 691 (/5735, 12.0%), more prevalent in males (447/3235 13.8%) and children (0–11 years) (429/2747, 15.6%). Compared to children, the risk of S. typhi infection was lower in adolescence (adjusted OR = 0.52; 95% CI: 0.42–0.66), adulthood (19–59 years; aOR = 0.30; 95% CI: 0.25–0.38), and older adulthood (aOR = 0.08; 95% CI: 0.04–0.18) (p < 0.001). Compared to males, the risk of S. typhi infection was lower in females (aOR = 0.67; 95% CI = 0.56–0.80; p = 0.002). Living in a rural residence (compared to urban) was associated with a higher risk of infection (aOR = 1.38; 95% CI: 1.16–1.63; p = 0.001), while access to a groundwater source (compared to municipal water supply) led to a lower risk (aOR = 0.56; 95% CI: 0.43–0.73; p = 0.002). Vaccination demonstrated a robust protective effect (aOR = 0.069; 95% CI = 0.04–0.11, p = 0.002). For those with typhoid infections, clinical biomarker analysis revealed the presence of leucopenia (65/691, 9.4%), thrombocytopenia (130/691, 18.8%), and elevated alanine aminotransferase (ALT) (402/691, 58.2%) and C-reactive protein (CRP) (690/691, 99.9%) levels. Worryingly, among the positive S. typhi isolates, there was a high prevalence of drug resistance (653/691), including multidrug-resistant (MDR 82/691, 11.9%) and extensively drug-resistant types (XDR, 571/691, 82.6%). Conclusions: This study highlights the importance of age, sex, locality, water source, and vaccination status in shaping the epidemiological landscape of S. typhi in the Peshawar district. It implies that expanding vaccination coverage to the broader population of Khyber Pakhtunkhwa province, particularly in the district of Peshawar, would be beneficial. Full article

Thiamethoxam, a second-generation neonicotinoid insecticide is widely used for controlling sap-sucking insect pests including Rhopalosiphum padi. The current study aimed to investigate the life-history parameters and feeding behavior of R. padi following treatments with sublethal concentrations of thiamethoxam. The lethal concentration 50 (LC₅₀) value of thiamethoxam against adult R. padi was 11.458 mg L⁻¹ after 48 h exposure. The sublethal concentrations of thiamethoxam (LC₅ and LC₁₀) significantly decreased the adult longevity, fecundity, and reproductive days in the directly exposed aphids (F₀ generation). In the progeny R. padi (F₁), the developmental durations and total prereproductive period (TPRP) were decreased while the adult longevity, fecundity, and reproductive days (RP_d) were increased at both thiamethoxam concentrations. The demographic parameters including the net reproductive rate (R₀), intrinsic rate of increase (r), and finite rate of increase (λ) were prolonged only at the LC₅ of thiamethoxam. The EPG results indicated that the sublethal concentrations of thiamethoxam increases the total duration of non-probing (Np) while reducing the total duration of E2 in directly exposed aphids (F₀). Interestingly, the E2 were significantly increased in the progeny generation (F₁) descending from previously exposed parental aphids (F₀). Overall, this study showed that thiamethoxam exhibited high toxicity against directly exposed individuals (F₀), while inducing intergenerational hormetic effects on the progeny generation (F₁) of R. padi. These findings provided crucial details about thiamethoxam-induced hormetic effects that might be useful in managing resurgences of this key pest. Full article

Potentilla nepalensis belongs to the Rosaceae family and has numerous therapeutic applications as potent plant-based medicine. Forty phytoconstituents (PCs) from the root and stem through n-hexane (NR and NS) and methanolic (MR and MS) extracts were identified in earlier studies. However, the PCs affecting human genes and their roles in the body have not previously been disclosed. In this study, we employed network pharmacology, molecular docking, molecular dynamics simulations (MDSs), and MMGBSA methodologies. The SMILES format of PCs from the PubChem was used as input to DIGEP-Pred, with 764 identified as the inducing genes. Their enrichment studies have shown inducing genes’ gene ontology descriptions, involved pathways, associated diseases, and drugs. PPI networks constructed in String DB and network topological analyzing parameters performed in Cytoscape v3.10 revealed three therapeutic targets: TP53 from MS-, NR-, and NS-induced genes; HSPCB and Nf-kB1 from MR-induced genes. From 40 PCs, two PCs, 1b (MR) and 2a (MS), showed better binding scores (kcal/mol) with p53 protein of −8.6 and −8.0, and three PCs, 3a, (NR) 4a, and 4c (NS), with HSP protein of −9.6, −8.7, and −8.2. MDS and MMGBSA revealed these complexes are stable without higher deviations with better free energy values. Therapeutic targets identified in this study have a prominent role in numerous cancers. Thus, further investigations such as in vivo and in vitro studies should be carried out to find the molecular functions and interlaying mechanism of the identified therapeutic targets on numerous cancer cell lines in considering the PCs of P. nepalensis. Full article

Fossil fuel and electricity-powered irrigation techniques boost the water availability expense and increase greenhouse gas emissions. Especially in developing countries, solar-powered irrigation is becoming more popular as a response to the growing energy and environmental issues associated with agriculture systems. The existing study used data from 1080 wheat farmers in Balochistan, Pakistan, to investigate the impact of solar-powered irrigation system (SPIS) usage on the technical efficiency (TE) of wheat production. The TE of wheat production is estimated using a stochastic frontier production function, and the potential self-selectivity bias is addressed using an endogenous switching regression model. The findings indicated that 13.7% of the wheat cultivators assessed used SPISs to produce their crops. Using an endogenous switching regression model shows that SPIS usage increases the TE of wheat growers by 6.657%, after controlling for self-selection bias. While using SPISs, wheat farmers with large farms and farming familiarity had stronger positive effects on TE. The results highlight the need for more investigation and research into evidence-based good practice for SPIS solutions at the site level to ensure that the rollout of modern equipment not only drives the energy sector forward but also contributes significantly to our level playing field and sustainable environment. Full article

Background: A substantial portion of the world’s population owns and utilizes computers and mobile devices, contributing to the rapid expansion of digital advertising. Marketers swiftly recognized the communicative benefits of social media platforms like Facebook, YouTube, Twitter, Instagram, Snapchat, Pinterest, and LinkedIn. Considering the importance of social media platforms and digital modes of marketing, it is considered especially significant for small firms to integrate these platforms into their business strategies in order to improve performance. Methods: Based on this aim, this study collected data from 363 owners/managers of SMEs in Pakistan. Structural equation modeling is used to check the hypothesized model of the study. Results: The results show that compatibility, owner/manager support, employee IT skills, financial cost, government policies, and social influence significantly affect adoption of digital marketing by SMEs in Pakistan. Conclusions: Furthermore, digital marketing also positively affects SME performance. This paper discusses the study’s findings as well as managerial and academic implications, including its limitations and future research avenues. Full article

Photocatalysis is a promising technology for the degradation of recalcitrant organic pollutants in water. In this study, superparamagnetic zinc ferrite nanoparticles (ZnFe₂O₄) were synthesized and characterized using scanning electron microscopy, X-ray diffraction, energy dispersive X-ray and Fourier transform infrared spectroscopy. The synthesized nanoparticles (NPs) of ZnFe₂O₄ were observed to have a photosensitive nature and showed characteristic visible-light-induced activation that was used for the photocatalytic degradation of a textile dye, Remazol brilliant violet 5R (RBV-5R). The effect of different operational parameters such as pH, H₂O₂, catalyst dosage, concentration of RBV-5R and the reusability of ZnFe₂O₄ as well as scavengers were investigated under visible irradiation. The almost complete degradation (99.9%) of RBV-5R was observed at pH 10, 0.1 g of ZnFe₂O₄ and 6 mM of H₂O₂ in 30 min. The photocatalytic degradation of RBV-5R followed pseudo-first-order kinetics. The mineralization was calculated from total organic carbon (TOC) that was represented by 82% TOC removal in 30 min. The results revealed that visible-light-induced ZnFe₂O₄ photocatalysis may be a promising technology for the elimination of toxic organic dyes, such as RBV-5R, from water resources. Full article

The growth and productivity of wheat crops depend on the availability of essential nutrients such as zinc (Zn) and potassium (K₂O), which play critical roles in the plant’s physiological and biochemical processes. This study aimed to investigate the synergizing effect of zinc and potassium fertilizers on uptake of both the nutrients, growth, yield, and quality of the Hashim-08 cultivar and local landrace, during the 2019–2020 growing season in Dera Ismail Khan, Pakistan. The experiment was designed using a split plot pattern in a randomized complete pattern, with main plots for the wheat cultivars and subplots for the fertilizer treatments. Results indicated that both cultivars responded positively to the fertilizer treatments, with the local landrace exhibiting maximum plant height and biological yield, and improved Hashim-08, showing increased agronomic parameters, including the number of tillers and grains and spike length. Application of Zn and K₂O fertilizers significantly enhanced agronomic parameters, such as the number of grains per plant, spike length, thousand-grain weight, grain yield, harvest index, Zn uptake of grain, dry gluten content, and grain moisture content, while crude protein and grain potassium remained relatively unchanged. The soil’s Zn and K content dynamics were found to vary among treatments. In conclusion, the combined application of Zn and K₂O fertilizers proved beneficial in improving the growth, yield, and quality of wheat crops, with the local landrace exhibiting lower grain yield but greater Zn uptake through fertilizer application. The study’s findings highlight that the local landrace showed good response to the growth and qualitative parameter when compared with the Hashim-08 cultivar. Additionally, the combined application of Zn and K showed a positive relation in terms of nutrient uptake and soil Zn and K content. Full article

A nonlinear mathematical model of COVID-19 containing asymptomatic as well as symptomatic classes of infected individuals is considered and examined in the current paper. The largest eigenvalue of the next-generation matrix known as the reproductive number is obtained for the model, and serves as an epidemic indicator. To better understand the dynamic behavior of the continuous model, the unconditionally stable nonstandard finite difference (NSFD) scheme is constructed. The aim of developing the NSFD scheme for differential equations is its dynamic reliability, which means discretizing the continuous model that retains important dynamic properties such as positivity of solutions and its convergence to equilibria of the continuous model for all finite step sizes. The Schur–Cohn criterion is used to address the local stability of disease-free and endemic equilibria for the NSFD scheme; however, global stability is determined by using Lyapunov function theory. We perform numerical simulations using various values of some key parameters to see more characteristics of the state variables and to support our theoretical findings. The numerical simulations confirm that the discrete NSFD scheme maintains all the dynamic features of the continuous model. Full article

Biochar is an important soil amendment that can enhance the biological properties of soil, as well as nitrogen (N) uptake and utilization in N-fertilized crops. However, few studies have characterized the effects of urea and biochar application on soil biochemical traits and its effect on paddy rice. Therefore, a field trial was conducted in the early and late seasons of 2020 in a randomized complete block design with two N levels (135 and 180 kg ha⁻¹) and four levels of biochar (0, 10, 20, and 30 t ha⁻¹). The treatment combinations were as follows: 135 kg N ha⁻¹ + 0 t B ha⁻¹ (T1), 135 kg N ha⁻¹ + 10 t B ha⁻¹ (T2), 135 kg N ha⁻¹ + 20 t B ha⁻¹ (T3), 135 kg N ha⁻¹ + 30 t B ha⁻¹ (T4), 180 kg N ha⁻¹ + 0 t B ha⁻¹ (T5), 180 kg N ha⁻¹ + 10 t B ha⁻¹ (T6), 180 kg N ha⁻¹ + 20 t B ha⁻¹ (T7) and 180 kg N ha⁻¹ + 30 t B ha⁻¹ (T8). The results showed that soil amended with biochar had higher soil pH, soil organic carbon content, total nitrogen content, and mineral nitrogen (NH₄⁺-N and NO₃⁻-N) than soil that had not been amended with biochar. In both seasons, the 20 t ha⁻¹ and 30 t ha⁻¹ biochar treatments had the highest an average concentrations of NO₃^–-N (10.54 mg kg⁻¹ and 10.25 mg kg⁻¹, respectively). In comparison to soil that had not been treated with biochar, the average activity of the enzymes urease, polyphenol oxidase, dehydrogenase, and chitinase was, respectively, 25.28%, 14.13%, 67.76%, and 22.26% greater; however, the activity of the enzyme catalase was 15.06% lower in both seasons. Application of biochar considerably increased the abundance of ammonia-oxidizing bacteria (AOB), which was 48% greater on average in biochar-amended soil than in unamended soil. However, there were no significant variations in the abundances of ammonia-oxidizing archaea (AOA) or nitrite-oxidizing bacteria (NOB) across treatments. In comparison to soil that had not been treated with biochar, the average N content was 24.46%, 20.47%, and 19.08% higher in the stem, leaves, and panicles, respectively. In general, adding biochar at a rate of 20 to 30 t ha⁻¹ with low-dose urea (135 kg N ha⁻¹) is a beneficial technique for improving the nutrient balance and biological processes of soil, as well as the N uptake and grain yield of rice plants. Full article

Silybum marianum has been used for a variety of purposes all over the world. This plant is used to treat cancer, liver disease, and inflammation. Eleven endophytic bacteria were isolated from S. marianum collected from heavy metal polluted sites and identified using 16s rRNA sequencing in the current investigation. Three isolates stood out for having many features, including heavy metal resistance, plant growth stimulation, plant hormones, heavy metal toxicity remediation, and antibacterial action. SJLC (6.06 µg/L) and SJRB (5.98 µg/L) were the greatest producers of IAA among the isolates and were identified as Bacillus sp. and Lysinibacillus sp., respectively. Root and shoot length have improved as a result of IAA production. The SJLC was found to be effective against four of the pathogens tested. The strain SJLC showed the most activity against Bacillus cereus with a 20 mm zone of inhibition, followed by the isolate SJRB, which showed a 16 mm zone of inhibition against B. cereus. The same isolates also show inhibition against X. campestris. Almost majority of the Brassica napus plants inoculated with bacterial isolates were able to translocate and degrade heavy metals like Cr. Therefore, it was concluded that these isolates are capable to grow in highly polluted environments. Full article

Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the association of MCM3 expression, its clinicopathologic parameters and to identify snuff (also called naswar) as a potential risk factor for changes in MCM3 expression in PMOL and OSCC. Methodology: Immunohistochemistry (IHC) of MCM3 was performed on 32 PMOL, 32 OSCC and 16 normal controls after optimization of IHC methodology. Histoscore (0–300) was used as a scoring system and seven different cut-offs were identified for analyses. Data were analyzed using various statistical tests. Results: Among the seven cutoffs, 40% strong positive cells were found to be a better cut-off as they were associated with many pathological variables (Broder’s grade, Aneroth’s grade, and mitotic activity). The differential MCM3 expression in oral lesions (PMOL and OSCC) was statistically significant (p = 0.03). Moreover, MCM3 expression is raised with increased duration and frequency of snuff use. Conclusion: High MCM3 expression is associated with disease progression and is a potential indicator of malignant transformations from PMOL to OSCC. Moreover, the use of snuff is associated with MCM3 over-expression. Full article