Search Results (56)

Ceramic dental prosthetics with internal metal structures are made from a cobalt–chromium alloy that is coated with ceramic. This study aims to validate surface treatments for the metal that enhance the adhesion of the ceramic coating under masticatory forces. Surface conditioning is performed using mechanical methods, like sandblasting (SB), and thermal methods, such as oxidation (O). The ceramic coating is applied to the metal component following the conditioning process, which can be conducted using either a single method or a combination of methods. Each conditioned sample undergoes characterization through various techniques, including drop shape analysis (DSA), scanning electron microscopy (SEM), X-ray diffraction (EDX), and atomic force microscopy (AFM). After the ceramic coating is applied and subjected to thermal sintering, the metal–ceramic samples are mechanically tested to assess the adhesion of the ceramic layer. The research findings, illustrated by scanning electron microscopy (SEM) images of the metal structures’ surfaces, indicate that alloy powder particles ranging from 10 to 50 µm were either adhered to the surfaces or present as discrete dots. Particles that exceed the initial design specifications of the structure can be smoothed out using sandblasting or mechanical finishing techniques. The energy-dispersive spectroscopy (EDS) results show that, after sandblasting, fragments of aluminum oxide remain trapped on the surface of the metal structures. These remnants are considered impurities, which can negatively impact the adhesion of the ceramic to the metal substrate. The analysis focuses on the exfoliation of the ceramic material from the deformed metal surfaces. The results emphasize the significant role of the sandblasting method and the micro-topography it creates, as well as the importance of the oxidation temperature in the treatment process. Drawing on 25 years of experience in dental prosthetics and the findings from this study, this publication aims to serve as a guide for applying the ceramic bonding layer to metal surfaces and for conditioning methods. These practices are essential for enhancing the adhesion of ceramic materials to metal substrates. Full article

Hydroxyapatite (HAP) is the most widely accepted biomaterial for repairing bone tissue defects, demonstrating excellent biocompatibility and bioactivity that promote new bone formation. Zirconia (ZrO₂), known for its strength and fracture toughness, is commonly used to reinforce ceramics. In this study, magnesium oxide (MgO) served as a stabilizer for zirconia, resulting in magnesia partially stabilized zirconia (Mg-PSZ). Both Mg-PSZ and HAP were synthesized via coprecipitation and mixed in specific ratios to create composites through a ceramic method involving mixing, compaction, and sintering at 1100 °C. The samples were characterized using techniques such as X-ray powder diffraction (XRPD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDS). Structural analyses confirmed the presence of both monoclinic and tetragonal zirconia phases. Besides, the increased wt.% HAP in the composites produced distinct peaks for hexagonal HAP. Crystallite sizes ranged from 27.45 nm to 31.5 nm, and surface morphology was homogeneous with small pores. Elements such as calcium, phosphorus, magnesium, zirconium, and oxygen were detected in all samples. This research also examined microhardness changes in the materials. The findings revealed enhancement in microhardness for the biocomposite with higher zirconia content, 90Mg-PSZ/10HAP sample, with the smallest average pore size, highlighting its potential for biomedical applications. Full article

Background: The autogenous bone core block (BCB) is a viable, biologically advantageous, and minimally invasive alternative to other augmentation procedures for small bone defects around dental implants. This study focused specifically on horizontal vestibular defects in the mandible, a frequently encountered yet underrepresented clinical situation, to evaluate the effectiveness and predictability of bone core grafting. Methods: Cylindrical autogenous bone cores, harvested from the implant-site osteotomy using trephine drills with a 2.5 internal diameter, were stabilized with osteosynthesis screws, and implants were placed simultaneously. Initial preoperative measurements of the edentulous ridge width were performed based on cone beam computer tomography (CBCT). At 4 months postoperatively, a subsequent CBCT measurement was performed for each implant site. Results: A total of 38 augmentation procedures were analyzed with a mean horizontal bone gain of 1.8 mm (p = 0.000). Improved outcomes were observed in V-shaped defects with remaining vertical bony walls, which contributed to better graft stability and volume preservation. While Khoury et al. previously validated the general applicability of this technique across various defect types, our study refines its indication by offering a clear protocol tailored to a common clinical niche. Conclusions: The proposed BCB method proved to be a safe, efficient, and with reduced morbidity procedure, providing clinicians with a practical and evidence-based tool for predictable horizontal bone augmentation. Full article

Nosocomial fungal infections caused by Candida auris pose a threat to public health due to their increased resistance to common antifungal drugs. Four thiourea derivatives of 2-thiophenecarboxylic acid were evaluated for their antifungal and antioxidant activity. The antifungal activity of the compounds was tested against strains of C. auris isolated from a hospital in Romania. With a notable inhibitory effect on C. auris biofilm growth and microbial adherence, the ortho-methylated derivative (SB2) showed the highest antifungal activity. Furthermore, emphasizing the impact of structural factors on the electron-donating capacity of these compounds, antioxidant activity assays (DPPH, FRAP, TEAC and CUPRAC) identified the SB2 compound as having the highest antihemolytic and antioxidant effects. The low cytotoxicity validated by hemocompatibility assays makes these compounds options for antifungal treatment. The results show that antifungal and antioxidant action is greatly influenced by structural modifications, especially the position of the methyl group on the aromatic ring. The possible clinical uses of these molecules as drugs for the treatment of multidrug-resistant C. auris infections needs further investigation. Full article

(1) Background: Exploring real-world data (RWD) regarding immune-related adverse events (irAEs) is crucial to better understand the efficacy and safety of immunotherapy in cancer patient populations excluded from clinical trials. An analysis was conducted to evaluate the presumptive predictive causality between endocrine irAEs and the efficacy of immune check-point inhibitors (ICIs) in metastatic non-small-cell lung cancer (mNSCLC) patients treated in daily practice in Romania. (2) Methods: This was a retrospective cohort study of mNSCLC patients treated with ICIs in a tertiary level hospital in Romania for a period of almost seven years, from November 2017 till July 2024. Endocrine irAEs were well defined as any occurring autoimmune endocrinopathy during ICIs and related to immunotherapy. The hospital endocrinologist (M.C.C.O) diagnosed, treated, and followed these endocrine irAEs in a multidisciplinary approach. We investigated multiple medical variables to assess their impact on ICI effectiveness. Descriptive and statistical analyses were performed. (3) Results: Of 487 cancer patients treated with ICIs, we identified 215 mNSCLC patients who were evaluated for endocrine irAEs and co-medications during ICI therapy. Forty-seven (21.8%) patients experienced endocrine irAEs, thyroiditis being the most frequent and prevalent autoimmune endocrinopathy in 60% of cases. Endocrine irAEs were statistically significant, correlated with ICI efficacy (p = 0.002) for survival analysis. Steroids and proton-pump inhibitors used as co-medication during ICIs had a negative impact on response to therapy. (4) Conclusions: Endocrine irAEs might be considered predictive biomarkers for successful immunotherapy in mNSCLC patients. Co-medication during ICIs had a major influence on the effectiveness of these cutting-edge therapies. RWD plays an important role for oncology daily practice whenever clinical trial evidence is not available to guide decision. Full article

This paper describes the synthesis and evaluation of samarium-doped 45S5 bioactive glass in various ratios. The bioactive glass samples were prepared using the sol–gel method and subjected to a heat treatment at 700 °C in normal atmosphere. The obtained samples were analyzed by thermogravimetric analysis (TGA) before and after the heat treatment to assess their thermal stability and compositional changes. The bioactivity of the samples was tested in vitro by immersion in simulated body fluid (SBF) at 36.5 ± 0.5 °C (normal human body temperature) and pH 7.4 (the pH of the human blood plasma), for several time periods. During the test, the pH and conductivity of the SBF solutions were monitored to track ion migration. After the in vitro test, the mass loss was evaluated and the formation of hydroxycarbonate apatite (HCA) was analyzed by FTIR spectroscopy. The microstructure of the bioactive glasses was examined using scanning electron microscopy (SEM) and the density of bioactive glass was also determined using Archimedes’ principle. This study also investigated the antimicrobial and anti-biofilm properties of both undoped and samarium-doped 45S5 bioactive glass through qualitative and quantitative assays against a range of microorganisms, including Gram-negative, Gram-positive, and yeast reference strains. The results were compared with literature data on melt-derived bioactive glass to evaluate the effects of Sm doping and the sol–gel synthesis method on bioactive glass performance. Full article

Background and Clinical Significance: Lung cancer, a leading cause of global cancer diagnoses, maintains the highest mortality risk despite advances in treatment. Immunotherapy agents, such as anti-programmed death-1/programmed death ligand-1 (PD-1/PD-L1), have revolutionized care for non-small cell lung cancer (NSCLC). However, the success is tempered by the emergence of immune-mediated adverse reactions, including the rare onset of type I diabetes. The incidence of diabetes mellitus increased during the SARS-CoV-2 pandemic. While there are several cases of new-onset diabetes after COVID-19 and COVID-19 vaccination, no case of new-onset type 1 diabetes after COVID-19 was described in an immune checkpoint inhibitor (ICI)-treated patient. Case Presentation: A 57-year-old male with stage IV NSCLC (brain and liver metastases) who had been treated with nivolumab for 4 years appeared positive for SARS-CoV-2 infection at a routine check. After two weeks, he was admitted to our clinic with severe fatigue, hyperglycemia, hyponatremia, and hyperkalemia. HbA1c level was normal and serum peptide C was undetectable. Nivolumab treatment was ceased, and the patient became fully dependent on basal–bolus insulin. After 3 months, the patient showed a complete imagistic remission. Conclusions: The case presented significant challenges due to the unclear etiology of newly onset diabetes and the uncommon age at which type 1 diabetes is developed. The outcome suggests that anti-PD-1 and SARS-CoV-2 infection can act synergistically. Full article

Maintaining effluent quality in wastewater treatment plants (WWTPs) comes with significant challenges under variable weather conditions, where sudden changes in flow rate and increased pollutant loads can affect treatment performance. Traditional physical sensors became both expensive and susceptible to failure under extreme conditions. In this study, we evaluate the performance of soft sensors based on artificial intelligence (AI) to predict the components underlying the calculation of the effluent quality index (EQI). We thus focus our study on three ML models: Long Short-Term Memory (LSTM), Gated Recurrent Unit (GRU) and Transformer. Using the Benchmark Simulation Model no. 2 (BSM2) as the WWTP, we were able to obtain datasets for training the ML models and to evaluate their performance in dry weather scenarios, rainy episodes, and storm events. To improve the classification of networks according to the type of weather, we developed a Random Forest (RF)-based meta-classifier. The results indicate that for dry weather conditions the Transformer network achieved the best performance, while for rain episodes and storm scenarios the GRU was able to capture sudden variations with the highest accuracy. LSTM performed normally in stable conditions but struggled with rapid fluctuations. These results support the decision to integrate AI-based predictive models in WWTPs, highlighting the top performances of both a recurrent network (GRU) and a feed-forward network (Transformer) in obtaining effluent quality predictions under different weather conditions. Full article

In the context of economic crises and inflationary pressures, consumers often rely on discounted food products to manage their monthly budgets. This study aims to explore how socio-demographic factors are associated with Romanian consumers’ perception of food promotions. Data were collected from 1060 respondents, and the analysis was conducted using R version 4.4.2, applying both descriptive and inferential statistical methods, including Pearson’s chi-square test and multinomial logistic regression. The Pearson’s chi-square test revealed significant differences in consumer responses based on all socio-demographic factors examined, except for residence. Males, married individuals, those with higher education, and higher net incomes were generally more cautious about promotions, while younger consumers (aged 18–24) showed greater receptiveness. The multinomial logistic regression further identified significant predictors and estimated their impact on consumers’ perception of food promotions. We found that gender, marital status, education level, and age were strong predictors, while income had only a quadratic impact, and residence showed no statistically significant outcome. These findings offer valuable insights for shaping marketing strategies and highlight the role of socio-demographic factors in shaping consumer perceptions toward food promotions. Full article

Background/Objectives: This study aims to characterize antibiotic resistance (AR) and virulence markers in Salmonella spp. isolated from Romanian outpatients’ stool samples. Methods: In 2019, community-acquired Salmonella strains were collected and identified using MALDI-TOF mass spectrometry, antibiotic susceptibility profiles have been determined with the MicroScan system, and soluble virulence factors were evaluated using specific culture media, while biofilm formation was quantified in 96-well plates. Molecular analysis targeted resistance genes for β-lactams (e.g., bla_TEM and bla_SHV); tetracyclines (e.g., tet(A)); sulphonamides; and quinolones, as well as virulence genes (e.g., invA, spvC, pldA, and held). Whole-genome sequencing (WGS) was performed on 19 selected isolates. A silver nanoparticles (AgNPsol) alternative to conventional antibiotics was tested for effectiveness against multidrug-resistant (MDR) isolates. Results: From the total of 309 Salmonella isolates (65.05% from children under 4 years of age) belonging to four subtypes and four serovars, 27.86% showed resistance to at least one antibiotic, most frequently to tetracycline, ampicillin, and piperacillin. The strains frequently expressed haemolysin (67%), aesculinase (65%), and gelatinase (62%). Resistance to trimethoprim-sulfamethoxazole was encoded by the sul1 gene in 44.83% of the strains and to tetracyclines by the tet(A) gene (59.52%). The ESBL genes bla_TEM, bla_SHV, and bla_CTX-M were detected by PCR in 16.18%, 2.91%, and 0.65% of the strains, respectively. Additionally, 98.63% of the strains carried the invA marker, with notable positive associations between bla_SHV, qnrB, and sul1 with spvC. Conclusions: The present findings revealed significant patterns in Salmonella isolates, subtypes, serovars, AR, and virulence, emphasising the need for continuous surveillance of Salmonella infections. Additionally, the potential of AgNPs as an alternative treatment option was demonstrated, particularly for paediatric S. enterica infections. Full article

In a dynamic context, where market requirements and needs change often, it is important for companies to adapt to new demands as quickly as possible to continue to be successful. To be able to face numerous challenges, however, organizations need to focus on the needs of their employees, with their satisfaction being an intermediate objective in achieving performance. Since technology is a pillar of each business field, the aim of this study is to explore employees’ perspectives on the use of technology at work, analyzing its main roles within the company, the benefits it provides from the employees’ viewpoint and its implications for satisfaction and performance. To achieve this objective, the authors used existing results in the literature that indicated several methods for the analysis of this subject, and continued with qualitative focus group research that involved six employees working both in hybrid and remote setups, within companies providing services in the engineering area. Thus, in this qualitative study, the needs and preferences of engineering employees are analyzed towards the efficiency of work activities, the improvement of team collaboration, as well as the role of digital technologies in facilitating communication and collecting feedback. Also, the research results address the support of personal and professional development and emphasize the importance of balance between personal and professional life through workplace flexibility—an aspect relatively unexplored in the specialized literature. Apart from the theoretical contributions that the study offers for the development of the literature, the results of this research offer a practical perspective for companies in the field of engineering, suggesting future research directions and recommendations for optimizing performance and increasing the level of employee satisfaction through the implementation of appropriate and personalized digital solutions. Full article

Background: Conditions associated with BRCA1/2 pathogenic (PVs) or likely pathogenic variants (LPVs) are often severe. The early detection of carrier status is ideal, as it provides options for effective case management. Materials and Methods: The study involved 58 patients with a personal and familial history of breast cancer (BC) who underwent genetic testing at the Regional Centre for Medical Genetics Dolj over a three-year period. An immunohistochemical panel (HER2, ER, PR, and Ki-67) was used to define the molecular subtypes of breast tumors. The AmpliSeq for Illumina BRCA Panel was used to evaluate germline variants in the BRCA1 and BRCA2 genes in patients with BC. The χ² test and Fisher’s exact test were used to compare the different parameters studied. Results: Our findings revealed that 15.5% of the patients carried either BRCA1 or BRCA2 PVs or LPVs. BRCA1 carriers had aggressive tumors whereas BRCA2 carriers had rather low-grade tumors. Conclusions: The study revealed that PVs in both BRCA genes have a significant frequency among BC patients in our region, and BRCA1 carriers tend to develop more aggressive tumors than carriers of BRCA2 PVs and patients with no germline PVs in either of the two genes. These observations could provide new epidemiologic data for this disease in our region and contribute further to the development of national screening strategies. Full article

Background/Objectives: We aimed to characterize the fibrosis following COVID-19 pneumonia, using quantitative analysis, after three months and subsequently, after two years of patients’ release from the hospital, and to identify the risk factors for pulmonary fibrosis. Methods: We performed a retrospective, observational cohort study on 420 patients with severe forms of COVID-19. For all patients, we registered demographic, inflammatory and biochemical parameters, complete blood count and D-dimers; all patients underwent three computed tomography scans (at admittance, at 3 months and at 2 years). Results: We found fibrosis in 67.9% of patients at the 3-month evaluation and in 42.4% of patients at the 2-year evaluation, registering a significant decrease in the severe and moderate fibrosis cases, with a slight increase in the mild fibrosis cases. The risk of fibrosis was found to be proportional to the values of age, duration of hospital stay, inflammatory markers (ESR, fibrinogen), cytolytic markers (LDH, AST) and D-dimers. The highest correlations with lung fibrosis were registered for interstitial pulmonary involvement (for the 3-month evaluation) and total pulmonary involvement (for the 2-year evaluation). Conclusions: Lung fibrosis represents a significant post-COVID-19 complication found in 42% of patients with severe forms of pneumonia at the 2-year evaluation. A significant overall decrease in the severity of lung fibrosis was registered at the 2-year evaluation compared to the 3-month evaluation. We consider that the amount of interstitial pulmonary involvement represents the optimal parameter to estimate the risk of lung fibrosis following SARS-CoV-2 pneumonia. Full article

Background/Objectives: This review aims to provide a comprehensive understanding of how HIV alters normal aging trajectories in the brain, presenting the HIV-related molecular mechanisms and pathophysiological pathways involved in brain aging. The review explores the roles of inflammation, oxidative stress, and viral persistence in the brain, highlighting how these factors contribute to neuronal damage and cognitive impairment and accelerate normal brain aging. Additionally, it also addresses the impact of antiretroviral therapy on brain aging and the biological markers associated with its occurrence. Methods: We extensively searched PubMed for English-language articles published from 2000 to 2024. The following keywords were used in the search: “HIV”, “brain”, “brain aging”, “neuroinflammation”, “HAART”, and “HAND”. This strategy yielded 250 articles for inclusion in our review. Results: A combination of blood-brain barrier dysfunction, with the direct effects of HIV on the central nervous system, chronic neuroinflammation, telomere shortening, neurogenesis impairments, and neurotoxicity associated with antiretroviral treatment (ART), alters and amplifies the mechanisms of normal brain aging. Conclusions: Current evidence suggests that HIV infection accelerates neurodegenerative processes of normal brain aging, leading to cognitive decline and structural brain changes at an earlier age than typically observed in the general population. Full article

Acute limb ischemia is a limb-threatening condition that is associated with a high degree of mortality and morbidity, with the latter related to acute kidney injury and rhabdomyolysis that can rapidly lead to multiple organ failure. The aim of this study was to assess the efficacy and safety of catheter-directed arterial thrombolysis in acute lower limb ischemia in the Department of Vascular Surgery, Timișoara, Romania. A total of 158 patients (114 males—72.15% and 44 females—27.85%) with symptoms of acute lower limb ischemia were admitted and treated with catheter-directed arterial thrombolysis following our protocol. The amputation-free survival rate at 1 month after the thrombolysis was 82.3%, and at 6 months it was 77.85%. The performance of additional procedures to obtain distal perfusion was predictive of an improved outcome at 30 days. The estimated survival rate at 6 months was 84.81% (SE 0.02). The mean survival time was 158.74 days. We recommend the usage of a thrombolytic regimen in patients with a life expectancy of more than 6 months, even in Rutherford stage IIb patients, if there is no major impairment in the sensorial and mobility function of the ischemic leg. Full article