Search Results (162)

Potentially toxic elements, such as chromium (Cr) and zinc (Zn), play essential roles in humans and animals. However, the harmful effects of excessive exposure to these elements through food remain unknown. In this sense, this study aimed to evaluate the anthropogenic contamination of chromium and zinc in aquatic biota and seafood consumers. Based on the PRISMA protocol, 67 articles were selected for this systematic review. The main results point to a wide distribution of these elements, which have familiar emission sources in the aquatic environment, especially in highly industrialized regions. Significant concentrations of both have been reported in different fish species, which sometimes represent a non-carcinogenic risk to consumer health and a carcinogenic risk related to Cr exposure. New studies should be encouraged to fill gaps, such as the characterization of the toxicity of these essential elements through fish consumption, determination of limit concentrations updated by international regulatory institutions, especially for zinc, studies on the influence of abiotic factors on the toxicity and bioavailability of elements in the environment, and those that evaluate the bioaccessibility of these elements in a simulated digestion system when in high concentrations. Full article

Despite the current level of public immunity to SARS-CoV-2, the early inflammatory events associated with respiratory distress in COVID-19 patients are not fully elucidated. Syrian golden hamsters, facultative hibernators, recapitulate the phenotype of SARS-CoV-2-induced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—induced severe acute lung injury seen in patients. In this study, we describe the predominance of the innate immune response in hamsters inoculated with four different SARS-CoV-2 variants, underscoring phenotypic differences among them. Severe inflammatory lung injury was chronologically associated with acute and significant weight loss, mainly in animals inoculated with A.2 and Delta variants. Omicron-infected animals had lower overall histopathology scores compared to other variants. We highlight the central role of endothelial injury and activation in the pathogenesis of experimental SARS-CoV-2 infection in hamsters, characterised by the presence of proliferative type I and type II pneumocytes with abundant surfactant expression, thereby maintaining hyperinflated alveolar fields. Additionally, there was evidence of intrapulmonary lymphatic vessel proliferation, which was accompanied by a lack of detectable microthrombosis in the lung parenchyma. However, white microthrombi were observed in lymphatic vessels. Our findings suggest that the physiological compensatory mechanisms that maintain respiratory homeostasis in Golden Syrian hamsters prevent severe respiratory distress and death after SARS-CoV-2 infection. Full article

Leadership is essential for promoting the circular economy (CE) by fostering systemic thinking, promoting resource efficiency, and driving innovative practices that close material loops and minimize environmental impact. In addition, the principles of CE recommend changing production chains to balance economic development, sustainability, and social well-being. This study examines a tertiary packaging company’s circular economy implementation, by using a framework and a circular business model canvas (CBMC) and by identifying the challenges and opportunities for improving environmental management. The method was a participatory research case study using interviews and frameworks. In the context of this company, servitization in logistics was highlighted as an alternative to overcome growth challenges and to add value; however, the company faces significant challenges in implementing it. In addition, it is necessary to increase consciousness among those involved about the concept of the CE and its benefits to improve operational efficiency and review product design to expand environmental management. This research concludes that servitization (as a circular economy strategy), environmental education, and innovation practices can help companies achieve greater financial performance in the packaging industry and contribute to sustainable development goals—SDG achievements. Full article

This paper presents the application of four machine learning algorithms to segment suppliers in a real case. The algorithms used were K-Means, Hierarchical K-Means, Agglomerative Nesting (AGNES), and Fuzzy Clustering. The analyzed company has suppliers that have been clustered using responses such as the number of non-conformities, location, and quantity supplied, among others. The CRISP-DM methodology was used for the work development. The proposed methodology is important for both industry and academia, as it helps managers make decisions about the quality of their suppliers and compares the use of four different algorithms for this purpose, which is an important insight for new studies. The K-Means algorithm obtained the best performance both for the metrics obtained and the simplicity of use. It is important to highlight that no studies to date have been conducted using the four algorithms proposed here applied in an industrial case, and this work shows this application. The use of artificial intelligence in industry is essential in this Industry 4.0 era for companies to make decisions, i.e., to have ways to make better decisions using data-driven concepts. Full article

This paper presents a multi-criteria decision-making (MCDM) approach for optimizing a microgrid system to achieve Plus-Energy Building (PEB) performance at the University of Coimbra’s Electrical Engineering Department. Using Python 3.12.8, Rhino 7, and PVsyst 8.0.1, simulations considered architectural and visual constraints, with economic feasibility assessed through a TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) analysis. The system is projected to generate approximately 1 GWh annually, with a 98% probability of exceeding 1076 MWh based on Gaussian estimation. Consumption is estimated at 460 MWh, while a 3.8 MWh battery ensures up to 72 h of autonomy. Rooftop panels and green parking arrays, fixed at 13.5° and 59°, minimize visual impact while contributing a surplus of +160% energy injection (or a net surplus of +60% energy after self-consumption). Assuming a battery cost of EUR 200/kWh, each hour of energy storage for the building requires 61 kWh of extra capacity with a cost of 12,200 (EUR/hr.storage). Recognizing environmental variability, these figures represent cross-validated probabilistic estimates derived from both PVsyst and Monte Carlo simulation using Python, reinforcing confidence in system feasibility. A holistic photovoltaic optimization strategy balances technical, economic, and architectural factors, demonstrating the potential of PEBs as a sustainable energy solution for academic institutions. Full article

The Mixed Ombrophyllous Forest (MOF), inserted in the Atlantic Forest biome, is of great ecological value, with deficient management strategies. In this context, sustainable management helps to promote the regeneration and growth of individual trees and control others, while maintaining the natural forest structure. This study therefore aimed to discuss opportunities and limitations of biochar, produced from two species from the MOF, which are currently only utilized to a limited extent in the study area in southern Brazil. A slow pyrolysis process at a lab scale was designed, biochar was produced, and key properties were analyzed from Hovenia dulcis Thunb. (chosen as an invasive species) and Mimosa scabrella Benth. (chosen as a native, fast-growing species), including branches and stems. The results showed that branches of Mimosa scabrella (BMS) had the highest biochar yield (30.32 ± 0.3%) and the highest electrical conductivity (415.08 ± 24.75 mS cm⁻¹). Stems of Mimosa scabrella (SMS) showed the highest higher heating value (HHV—31.76 ± 0.01 MJ kg⁻¹), lower heating value (LHV—31.03 ± 0.01 MJ kg⁻¹), and energy yield (49.1%), while the branches of Hovenia dulcis (BHD) showed the lowest values. For the elemental analysis, SMS showed the best results, with the highest amount of fixed carbon (78.62 ± 0.22%) and carbon content (85.87 ± 0.083%), and consequently the lowest amount of ash (3.52 ± 0.08%). BHD showed a better water-holding capacity (303.26 ± 15.21%) and higher pH value (7.65 ± 0.14). The investigations conducted on the biochar from both species indicate a strong suitability of these woods for producing high-quality biochar. Full article

Mortar is widely used in civil construction. The inclusion of expanded clay as a lightweight aggregate reduces the density of mortar, enabling lighter structural elements and potentially lowering material and energy requirements during construction. This research aims to produce lightweight mortars by partially replacing fine aggregate with proportions of expanded clay. Six mortar formulations were prepared with varying proportions of expanded clay. The constituent materials of the mixtures and the mortars were characterized according to regulatory prescriptions. The results indicated that the increase in the replacement of fine aggregate with expanded clay reduced the consistency and density of the mass in the fresh state. No significant differences were observed in water absorption by immersion among the mortars in the hardened state. Regarding mechanical tests, most mortars’ tensile strength in bending remained stable. On the other hand, compressive strength decreased. The tensile adhesion was also reduced with the incorporation of expanded clay. After exposure to sodium sulfate solution, all tensile strength results in bending improved. The coefficient of the constructive quality indicated that the ideal replacement formulation is 20% expanded clay. These mortars represent a viable technical alternative, complying with current standards and contributing more efficiently and sustainably to civil construction. Full article

This study evaluated the effects of natural pigments, such as paprika extract and marigold flower extract, on performance, egg quality, carotenoid content in the yolk, and shelf life of Japanese quail eggs. The experiment included 160 birds, divided into 4 treatment groups, as follows: 0.8% paprika extract, 0.8% marigold flower extract, 0.8% paprika/marigold (P/M) mixture, and a control group. The experiment lasted 84 days. The inclusion of paprika, marigold, or their combination did not affect performance parameters, except for feed conversion, which was significantly poorer in the supplemented groups compared to the control. An improvement in yolk color was observed, particularly in the marigold group, which showed higher concentrations of β-carotene (40 g/kg). Yolk color intensity, measured using the L*, a*, and b* scales, was enhanced in all supplemented groups. During storage, yolk weight increased after 5.72 days, while albumen weight showed a significant reduction starting at 4.30 days, with a more pronounced decrease after 6.6 days. Albumen diameter expanded after 15.7 days of storage, and the Haugh unit was significantly affected from the 17th day onward. Yolk percentage decreased after 4.06 days, while albumen and eggshell percentages decreased after 4.10 and 3.41 days, respectively. These results suggest that paprika and marigold extracts are effective in improving yolk color and maintaining egg quality without compromising performance. However, a poorer feed conversion by mass was observed in the groups fed with the paprika, marigold, and the (P/M) mixture, compared to the control group. These natural pigments also positively impacted egg quality, particularly in terms of color intensity and internal parameters, and helped maintain egg quality during storage, meeting consumer expectations for high-quality products. Future studies should focus on evaluating the antioxidant effects of these pigments in eggs, as this could provide a deeper understanding of their potential benefits, both in terms of nutritional quality and shelf-life enhancement. Full article

Foam stabilization plays a critical role in the production of foamed mortar, a material widely applied in civil construction due to its thermal insulation and lightweight structural benefits. This study investigates the use of magnetite derived from acid mine drainage (AMD) as a sustainable foam-stabilizing agent. Magnetite’s magnetic properties enhance foam stability by improving air bubble distribution within the mortar. A total of 30 different mixtures were produced, varying the sand-to-cement ratio, type of cement and magnetite content. The compressive strength and tensile flexural strength of the foamed mortars ranged from 0.62 ± 0.04 MPa to 7.33 ± 0.30 MPa and from 0.44 ± 0.12 MPa to 2.82 ± 0.16 MPa, respectively; porosity ranged from 31.8% ± 1.86 to 75.6% ± 2.2; dry and wet bulk density ranged from 423 ± 23 kg.m⁻³ to 1576 ± 96 kg.m⁻³ and from 615 ± 9 kg.m⁻³ to 1828 ± 122 kg.m⁻³, respectively; water absorption ranged from 8.9% ± 0.9 to 45.8% ± 10.6; and thermal conductivity ranged from 0.54 ± 0.03 W·m⁻¹·K⁻¹ to 0.17 ± 0.03 W·m⁻¹·K⁻¹. Results demonstrated that increasing magnetite content led to greater foam stability and porosity but decreased mechanical strength and density. The sand-to-cement ratio significantly affected all measured properties, while the type of cement had minimal influence. These findings suggest that AMD-derived magnetite is a promising additive for optimizing the performance of lightweight, sustainable foamed mortars. Full article

Background/Objectives: The main biologically active molecules of Cannabis sativa L. are cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC). Both exert anticonvulsant effects when evaluated as single drugs, but their possible interaction as components of C. sativa extracts has been scarcely studied. For this reason, we evaluated CBD and THC, combined or not, in two seizure models in mice, using an improved vehicle formula. Methods: Firstly, acute seizures were induced by intraperitoneal (i.p.) pentylenetetrazole (PTZ, 80 mg/kg), and mice received CBD or THC at 1, 3, 6, and 10 mg/kg, or a CBD/THC 1:1 combination at 1.5, 3, and 6 mg/kg, per os (p.o.), one hour before PTZ administration. Secondly, mice received p.o. CBD (10 mg/kg), CBD/THC (1.5, 3, and 6 mg/kg), valproic acid (50 mg/kg), or vehicle (nanoemulsions without CBD or THC), one hour before PTZ (30 mg/kg, i.p.) every other day for 21 days. Behavioral, biochemical, and immunohistochemical analyses were performed to assess the response to PTZ, oxidative stress, and astroglial activation. Results: In the acute model, CBD and THC at 3–10 mg/kg, and their combinations, significantly increased latency to generalized seizures and death, and improved survival rates. In the chronic model, similarly to valproic acid, CBD 10 mg/kg and CBD/THC at 1.5 and 3 mg/kg delayed kindling acquisition, while CBD/THC 6 mg/kg had no effect. CBD and CBD/THC treatments reduced oxidative and nitrosative stress and attenuated astrogliosis, as indicated by decreased glial fibrillary acidic protein and GABA transporter 1 expression and increased inwardly rectifying potassium channel 4.1 expression in hippocampal regions. However, no cannabinoid treatment prevented the impairment in novel object recognition and Y maze tests. Conclusions: These findings support the potential role of cannabinoids in counteracting seizures, possibly by reducing oxidative stress and astrogliosis. The study also highlights the importance of nanoemulsions as a delivery vehicle to enhance cannabinoid effectiveness while considering the risks associated with direct cannabinoid receptor activation. Full article

The biodegradation of glyphosate by bacteria is an emerging bioremediation strategy necessitated by the intensive use of this herbicide in global agriculture. This study systematically reviews the literature to identify bacteria with the potential to degrade glyphosate. The PRISMA protocol was utilized, considering relevant articles identified in electronic databases such as PubMed, Scopus, and Science Direct. The research identified 34 eligible studies, highlighting the genera Bacillus, Pseudomonas, and Ochrobactrum as having the greatest potential for glyphosate degradation. These findings were based on analytical techniques such as High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR), which identified and quantified intermediate metabolites, primarily AMPA (aminomethylphosphonic acid), sarcosine, and glyoxylate. This investigation also addressed enzymatic efficiency in biodegradation, emphasizing enzymes like glyphosate oxidoreductase and C-P lyases. The results indicated that South and North America lead in publications on this topic, with Argentina and the United States being the main contributors, reflecting the intense use of glyphosate in these countries. Additionally, studies in Europe and Asia focused on microbial diversity, exploring various bacterial genera. This investigation revealed that despite the promising microbial potential, there are challenges related to environmental condition variations and the cost of large-scale implementation, indicating that continuous research and process optimization are essential for the effective and sustainable application of this biotechnology. Full article

This study analysed perceived exertion (RPE) and blood lactate ([La⁻]) responses to two resistance training protocols planned with high- (HLI) and low-load intensities combined with blood flow restriction (LLI+BFR). Fourteen trained adults (26.2 ± 2.6 years) performed the HLI and LLI+BFR protocols 48 h apart. The HLI was planned with 70% 1RM (one repetition to maximum), three sets, 12 repetitions, 60 seconds (s) of rest between sets and 120 s between exercises; LLI+BFR was performed at 30% 1RM, for three sets, 15 repetitions, and with 30 s of rest between sets and 180 s between exercises. Blood samples (for [La⁻] analysis) and RPE (Borg 0–10 scale) were collected in the first minute after each exercise. A two-way ANOVA compared RPE and [La⁻] responses between exercises in the same protocol, and between protocols comparing the same exercise. RPE increased from the first to the last half (involving upper-limbs and lower-limbs) of exercises in both protocols (p < 0.001). All exercises in HLI elicited higher RPE values than LLI+BFR (p < 0.001). Average RPE scores were higher in HLI than for LLI+BFR (8.1 ± 0.6 > 6.2 ± 1.1, p < 0.001). The [La⁻] also increased throughout the exercises, with a higher peak response in LLI+BFR than for HLI (9.8 ± 1.6 > 7.2 ± 1.3 mmol × L⁻¹, p < 0.01). Perceptual and metabolic responses during HLI and LLI+BFR training were distinguishable, despite both protocols characterising a high-intensity stimulus. Full article

Controlled-release fertilizers contain polymeric coatings that modify the dynamics of phosphorus (P) release in soil. This study aimed to characterize P release from physical mixtures between conventional and controlled-release fertilizers (CRFs), quantify soil P availability, and assess agronomic responses of coffee plants during the establishment phase. Two main types of P fertilizer were evaluated: conventional monoammonium phosphate (MAP) and a blend (physical mixture of conventional MAP and controlled-release P fertilizers). Both fertilizers were applied at 0, 134, 268, and 403 kg ha⁻¹ of P₂O₅. Our findings revealed a blend longevity of 3 and 6 months. P fertilization contributed to an increase in leaf area (1134.7 cm² plant⁻¹) and shoot biomass (602.8 kg ha⁻¹) and raised P in the soil (0.061 mg dm⁻³ per kg of P₂O₅ applied). P accumulation in the coffee plants ranged between 3 and 4 kg ha⁻¹. Other macronutrient accumulations in aerial parts were of the following ranges (in kg ha⁻¹): 47–60 for N, 36–46 for K, 18–22 for Ca, 5–7 for Mg, and 3–4 for S. Micronutrients accumulated (in g ha⁻¹): 454–657 for Fe; 117–160 for B; 117–149 for Mn; 58–71 for Cu; and 34–43 for Zn. Up to 74% of the nutrients were distributed in the leaves. We concluded that the use of blends did not impose any limitation on P nutrition for coffee plants and led to biomass gains (18.9%) in plagiotropic branches. P fertilization proved essential for supporting the initial growth of coffee plants and increasing coffee leaf area and P levels in the soil and promotes adequate levels of P accumulation in plants, leading to improvements in coffee crop nutrition in the establishment phase. Full article

Background/Objectives: Andiroba oil, extracted from Carapa guianensis seeds, possesses therapeutic properties including anti-inflammatory and wound healing effects. This study aimed to develop and characterize a nanoemulsion formulation containing andiroba oil (NeAnd) and to evaluate its cytotoxicity and wound healing potential in vitro. Methods: The oil was evaluated for acidity, antioxidant activity, and fatty acid composition. NeAnd was produced by ultrasonication and characterized using FTIR (Fourier transform infrared spectroscopy), Raman spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: NeAnd exhibited a spherical shape and stable physicochemical properties, with an average hydrodynamic diameter (HD) of 205.7 ± 3.9 nm, a polydispersity index (PdI) of 0.295 ± 0.05, a negative zeta potential of −4.16 ± 0.414 mV, and pH of approximately 6.5. These nanodroplets remained stable for 120 days when stored at 4 °C and maintained their parameters even under pH variations. FTIR and Raman analyses confirmed the presence of functional groups and the organization of fatty acid chains in NeAnd. Cell viability assays revealed no statistically significant differences in cytotoxicity at various concentrations (90–360 µg/mL) after 24 and 48 h. In scratch wound healing assays, NeAnd significantly enhanced wound closure (88.9%) compared to the PBS control (38%) and free andiroba oil (68.6%) in keratinocytes (p < 0.05). Conclusions: These promising findings indicate NeAnd as a potential nanophytomedicine for wound healing and tissue regeneration treatments. Full article

This paper focuses on the application of fractional calculus techniques in the identification and control of multivariable (multiple input—multiple output) systems (MIMO). By considering a previously reported experimental set-up similar to a greenhouse, this study proposes the open-loop identification of fractional order transfer functions relating to the controlled and manipulated variables, which were validated by experimental data. Afterward, the theoretical analysis of Fractional-order Proportional and Integral (FOPI) closed-loop control for this MIMO system was carried out. An important aspect concerns the use of Particle Swarm Optimization (PSO) metaheuristic algorithm for optimization tasks, both in parameter estimation and controller tuning. Moreover, comparisons with integer order models and controllers (IOPID-IMC) were performed. The results demonstrate the superior performance and robustness of the FOPI-PSO fractional control, which achieves up to 79.6% reduction in ITAE and 72.1% reduction in ITSE criteria. Without the need for explicit decouplers, the decentralized FOPI-PSO control structure demonstrated effective handling of interactions between the temperature and humidity control loops, simplifying the control design while maintaining performance. The fractional-order controllers exhibited robustness to measurement noise, as evidenced by stable and precise control responses in the presence of experimental uncertainties. Additionally, the optimized tuning of FOPI controllers implicitly compensated for disturbances and setpoint changes without requiring additional feedforward mechanisms. This study contributes to a better understanding of fractional calculus applications in designing FO–MIMO systems and provides a practical framework for addressing the identified gaps in the field. Full article